PHYTOCHEMICAL INVESTIGATION AND ANTIMICROBIAL PROPERTIES OF DIOSCOREA BULBIFERA TUBER

2017 ◽  
Vol 10 (12) ◽  
pp. 317
Author(s):  
Dahiya P
Keyword(s):  
Antimicrobial Properties ◽  
Inhibition Zone ◽  
Multidrug Resistant ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Phytochemical Investigation ◽  
Dioscorea Bulbifera ◽  
Acinetobacter Sp ◽  
Almost All

  Objective: The inhibitory properties of successive extracts from Dioscorea bulbifera (Dioscoreaceae) tubers have been evaluated for the presence of phytochemical constituents and antimicrobial efficacy against multidrug-resistant (MDR) clinical isolates was evaluated.Methods: The tuber of D. bulbifera was oven dried and extracted successively with n-hexane, chloroform, methanol, ethanol, and water. The antimicrobial potential of successive extracts against MDR isolates was studied by agar well-diffusion method. Qualitative phytochemical analysis was performed.Results: Qualitative phytochemical analysis demonstrated the presence of steroids, flavonoids, cardiac glycosides, saponins, and reducing sugars in almost all the extracts tested. Anthraquinones, phlobatanins, and tannins were not reported in any extracts tested. The in vitro antimicrobial activity of various solvents and water extracts of D. bulbifera was further investigated against ten MDR bacteria and three fungi, respectively. Aqueous and chloroform extracts were found to be more potent being capable of exerting significant inhibitory activities against the majority of the isolates such as Escherichia coli, Acinetobacter sp., Salmonella paratyphi, Klebsiella pneumoniae, and Candida albicans. The highest inhibitory activity was observed for K. pneumoniae with wide inhibition zone diameters (17 ± 0.15 mm), followed by E. coli 1(13 ± 0.11) mm, and Acinetobacter sp. (11 ± 0.12).Conclusion: Based on the present study, the extracts of D. bulbifera tubers have shown excellent activity against MDR microbial cultures tested. Further study is recommended for clinical evaluation, of the efficacy of crude extract in herbal medicine that can serve as a base for the development of novel potent drugs and phytomedicines.

scholarly journals The PHYTOCHEMICALS, ANTIOXIDANT AND ANTIMICROBIAL PROPERTIES OF Senna alata AND Senna tora LEAF EXTRACTS AGAINST BACTERIAL STRAINS CAUSING SKIN INFECTIONS

2019 ◽  
Vol 2 (1) ◽  
pp. 19
Author(s):  
Murni Halim
Keyword(s):  
Leaf Extract ◽  
Antimicrobial Properties ◽  
Inhibition Zone ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Leaf Extracts ◽  
Natural Drugs ◽  
Senna Alata

A study was carried out to screen for phytochemical constituents and assess the antioxidant and antimicrobial activities of Senna alata and Senna tora leaf extracts. The leaves were first dried at room temperature and 50°C in an oven prior to solvent extraction using ethanol and methanol. The in-vitro qualitative assays showed that both S. alata and S. tora leaf extracts contained bioactive and secondary metabolites components such as tannins, steroids, saponin, terpenoids, glycosides, flavonoids and phenols. The antioxidant activity and capacity test were carried out by conducting free radical of 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity and Ferric reduction antioxidant plasma (FRAP) assays. Both assays showed S. tora leaf extract has higher antioxidant capacity than S. alata leaf extract. The efficacy of these leaf extracts were tested against skin pathogens through agar well diffusion method. S. alata extract showed an inhibition zone (1.15 – 1.59 mm) against Pseudomonas aeruginosa while S. tora extracts exhibited a strong antimicrobial activity against S. epidermidis (inhibition zone of 12 – 16.94 mm) followed by P. aeruginosa (inhibition zone of 1 – 1.59 mm). Nonetheless, no inhibition zone was observed for S. aureus by both leaf extracts. The phytochemicals and antioxidant constituents as well as inhibitory potential on skin pathogens possessed by S. alata and S. tora leave highlighted their potential utilization in the development of natural drugs or cosmetics to treat skin related diseases or infections.

scholarly journals Antimicrobial Effect Of Honey Distributed Around Pasar Minggu Jakarta Selatan

2017 ◽  
Vol 8 (2) ◽  
pp. 101-106
Author(s):  
Ruth Elenora Kristanty ◽  
Junie Suriawati ◽  
Priyanto Dwi Nugroho
Keyword(s):  
Infectious Diseases ◽  
Food Product ◽  
Antimicrobial Effect ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Bacteria Growth ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Almost All

Honey is a highly nutritious food product and consumed by almost all the population in the world. It has a function as an antimicrobial. Staphylococcus aureus (S. aureus) is a common Gram-positive bacteria in food and Escherichia coli (E. coli) is a Gram-negative bacteria that often appears in environmental sanitation issues that both can cause infectious diseases.  Some infectious diseases can be treated with antimicrobials such as honey. The purpose of this study was to test the antimicrobial effects on honey products distributed in Pasar Minggu area. The antimicrobial effect test was performed in vitro using agar diffusion method by measuring the inhibition zone formed where the bacteria growth was inhibited by the presence of sample. The concentration of samples were 25%, 50%, 75%, and 100% (not diluted) and as aquades control. The results showed that honey tested with various dilution concentrations resulted inhibition zone and. The higher concentration of the inhibited zone zone showed antimicrobial activity against S. aureus and E. coli.

scholarly journals Comparative Phytochemical, Antioxidant and Antimicrobial Properties of Ficus capensis, Aristolochia ringens, Albizia zygia and Lannea welwitschii

2018 ◽  
Vol 16 (2) ◽  
pp. 147-157 ◽  
Author(s):  
Wilfred O Obonga ◽  
Philip F Uzor ◽  
Emmanuel O Ekwealor ◽  
Sampson C Nwabuko
Keyword(s):  
Antimicrobial Properties ◽  
Stem Bark ◽  
Salmonella Typhi ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Traditional Medicines ◽  
E Coli ◽  
Dpph Scavenging

The present study was aimed at a comparative study of the phytoconstituents, antioxidant and antimicrobial properties of four medicinal plants, Ficus capensis, Aristolochia ringens, Albizia zygia and Lannea welwitschii. The leaf of F. capensis and stem bark of A. ringens, A. zygia and L. welwitschii were extracted using methanol. Phytochemical analysis was done spectrophotometrically. Three in vitro antioxidant tests-hydrogen peroxide (H2O2), nitric oxide (NO) and 2, 2-diphenyl-1-picrylhydroxyl (DPPH) scavenging models were employed. Antimicrobial test was done by agar diffusion method against E. coli, Staphylococcus aureus, Klebsiela pneumonia, Bacillus subtilis, Pseudomonas aeruginosa, Salmonella typhi, Candida albicans and Aspergittus spp. Phytochemical analysis revealed that the most abundant phytoconstituents were flavonoids (F. capensis and A. zygia), reducing sugar, terpenoids, alkaloids and tannins. In the antioxidant models, A. zygia was found to produce the least IC50 in two of the models (NO and DPPH). Results of the antimicrobial tests showed that A. zygia showed a broader spectrum of activity than other plants. This study has shown that these plants possess antioxidant and antimicrobial activities which further justify their usage in traditional medicines. A. zygia featured prominently in these activities. Also flavonoids could be responsible for the bioactivities of these plants.Dhaka Univ. J. Pharm. Sci. 16(2): 147-157, 2017 (December)

In-vitro antimicrobial efficacy of certian herbal seeds essential oils against important poultry microbes

2015 ◽  
Author(s):  
Darshana B. Bhaisare ◽  
D. Thyagarajan ◽  
R. Richard Churchil ◽  
N. Punniamurthy
Keyword(s):  
Staphylococcus Aureus ◽  
Antiviral Activity ◽  
Essential Oils ◽  
Pasteurella Multocida ◽  
Antimicrobial Properties ◽  
Inhibition Zone ◽  
Salmonella Typhi ◽  
Fungal Species ◽  
Diffusion Method

Two in-vitro experiments were conducted to evaluate the antibacterial, antifungal and antiviral properties of essential oil of herbal seeds. In-vitro antimicrobial properties of essential oils of phytobiotics was determined by disc diffusion method against Escherichia coli, Staphylococcus aureus, Pasteurella multocida and Salmonella typhi and two fungi namely Aspergillus fumigatus and Candida albicans. Thyme oil had statistically similar or significantly (P<0.05) higher inhibition zone against all the bacterial and fungal species compared to standard antibiotic, chloramphenicol or antifungal drug, nystatin. Fenugreek oil was not found to be active against microbes compared to control. Fennel and cumin oils had statistically similar or significantly (P<0.05) higher inhibition zone against all microbes except Staphylococcus aureus and Salmonella typhi compared to control. Antiviral activity of essential oils was determined by anti-NDV assay against New Castle disease virus (LaSota). The results indicated that essential oils of herbal seeds do not possess antiviral activity against NDV LaSota virus at 1mg/ml concentration.

In vitro antimicrobial activity of the leaf extract of Harungana madagascariensis Lam. ex Poir. (Hypericaceae) against strains causing otitis externa in dogs and cats

2007 ◽  
Vol 55 (1) ◽  
pp. 97-105 ◽  
Author(s):  
B. Moulari ◽  
Y. Pellequer ◽  
J. Chaumont ◽  
Y. Guillaume ◽  
J. Millet
Keyword(s):  
Otitis Externa ◽  
Leaf Extract ◽  
Antimicrobial Agents ◽  
Antimicrobial Properties ◽  
Antimicrobial Activities ◽  
Phytochemical Analysis ◽  
Malassezia Pachydermatis ◽  
Staphylococcus Intermedius ◽  
Phytochemical Investigation

Otitis externa in dogs and cats is always caused by a combination of yeasts and bacteria, among which the most important are Malassezia pachydermatis, Staphylococcus intermedius and Pseudomonas species. These organisms often develop resistance to classical antimicrobial agents. Therefore, the aim of this study was to investigate the antimicrobial activities of an ethyl acetate leaf extract of Harungana madagascariensis against the organisms cited, to carry out the phytochemical investigation of this extract and to determine its bioactive chemical class using dilution techniques, the bioautography method and the standard phytochemical method described by Harborne (1973). Phytochemical analysis revealed the presence of saponins, tannins, flavonoids, alkaloids and anthracenic derivatives. The bioassay showed that the antimicrobial properties may be attributed to astilbin, a flavanone derivative identified on the basis of its spectroscopic data. The results suggest that the extract could be used in an antimicrobial preparation effective against the whole range of organisms incriminated in otitis externa in dogs and cats, with a minimal inhibitory concentration (MIC) of 250 μg/ml.

scholarly journals Phytochemical screening and antimicrobial assay of some Nepalese medicinal plants

1970 ◽  
Vol 9 (9) ◽  
pp. 90-92 ◽  
Author(s):  
Bijaya Laxmi Maharjan ◽  
Smritri Mainali ◽  
Bikash Baral
Keyword(s):  
Medicinal Plants ◽  
Plant Extracts ◽  
Pathogenic Bacteria ◽  
Antimicrobial Properties ◽  
Phytopathogenic Fungi ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Acinetobacter Species ◽  
Antimicrobial Assay

Medicinal plants constitute several bioactive compounds that are antimicrobial. Due to the extensive need of the new antimicrobial compounds of plant origin the study was conducted. The present paper deals with the qualitative phytochemical analysis and antimicrobial assay of methanolic extracts of four medicinal plants namely Butea monosperma (seed), Cissampelos pareira (root), Myrica esculenta (bark) and Selinum tenuifolium (root). The standard phytochemical test of the extracts revealed the presence of polyphenols, glycosides and coumarins in all the plant extracts. For the in-vitro antimicrobial assay, agar well diffusion method was employed against human pathogenic bacteria (11) and phytopathogenic fungi (5). The extracts of C. pareira, M. esculenta were effective against all the tested bacteria and fungi which indicated their broad spectrum antimicrobial potential. The extract of B. monosperma was effective against B. subtilis, S. typhi, Acinetobacter species, F. oxysporum, F. moniliformie, E. turticum and S. roulfsii. But the extract of S. tenuifolium was ineffective against all the tested bacteria but was effective against all the fungal strains revealing it to have more antifungal potential. The antimicrobial properties of these plant extracts may be due to the presence of various secondary metabolites. The results provided evidence that the studied plants might indeed be employed in the management of microbial infections. Key words: Plant extract; Phytochemicals; Antibacterial; Antifungal DOI: http://dx.doi.org/10.3126/sw.v9i9.5526 SW 2011; 9(9): 90-92

scholarly journals AKTIVITAS ANTIBAKTERI FILTRAT Streptomyces sp. KCM2 TERHADAP MULTIDRUG RESISTANT Acinetobacter baumannii SECARA IN VITRO

2017 ◽  
Vol 21 (1) ◽  
pp. 21
Author(s):  
NI KADEK LOSIANI ◽  
RETNO KAWURI ◽  
A. A. KETUT DARMADI
Keyword(s):  
Acinetobacter Baumannii ◽  
Inhibition Zone ◽  
Multidrug Resistant ◽  
Diffusion Method ◽  
Clinical Microbiology Laboratory ◽  
Significance Level ◽  
Streptomyces Sp ◽  
Randomized Design ◽  
Range Test

Acinetobacter baumannii is one of the pathogen which leads to multidrug resistant (MDR-A baumannii). This pathogen is often causing outbreaks of infections to the patients which are receiving treatment in the intensive care unit in hospital. This study aims to find out the antibacterial activity and the Minimum Inhibitory Concentration (MIC) of Streptomyces sp. KCM2 filtrate in inhibiting the growth of MDR-A baumannii. Streptomyces sp.KCM2 isolates was isolated in rhizosphere of white turmeric (Curcuma mango Val.) (Losiani et al., 2016). MDRA. baumannii isolates were obtained from stock culture in Clinical Microbiology Laboratory Sanglah Hospital in Denpasar. The antibacterial and the MIC test of Streptomyces sp. KCM2 filtrate was using the wells diffusion method. The research design was used Completely Randomized Design (CRD) and the inhibitory zone diameterdata of MIC test were analyzed by Analysis of Varian (ANOVA), and then continued by Duncan Multiple Range Test in significance level 5%. The results of this study showed that the Streptomyces sp. KCM2 filtrate was able inhi biting with diameter zone of 23,44 mm and MIC of filtrate was 4% (v/v) with inhibition zone diameter of 8.77 mm.

scholarly journals Phytochemical Analysis and in vitro Antibacterial Activities of Seagrass Enhalus acoroides against Staphylococcus aureus

2020 ◽  
Vol 7 (2) ◽  
pp. 85-91
Author(s):  
Desy Setyoningrum ◽  
Ade Yamindago ◽  
Syarifah Hikmah Julinda Sari ◽  
Maftuch Maftuch
Keyword(s):  
Staphylococcus Aureus ◽  
Bioactive Compounds ◽  
Crude Extract ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Enhalus Acoroides

Several studies of marine bioactive compounds have been carried out using seagrass. Enhalus acoroides is a type of seagrass that has bioactive compounds including alkaloids, flavonoids, tannins, steroids, and saponins that have potential as an antibacteria. The aim of study is to investigate the phytochemical compound contained in the crude extract of Enhalus acoroides and its antibacterial activities of Enhalus acoroides against Staphylococcus aureus. The seagrass was collected, washed, dried, grind and exposed to extraction by vacum rotary evaporator at temperature of 40°C and was analyzed for their phytoconstituents. Further, the crude extract was tested against pathogenic bacterial at different concentrations using disc diffusion method to determine the effect of bioactive compounds in E. acoroides to bacteria. In the present study, qualitative test of phytochemical from ethanol extract of Enhalus acoroides leaves contained phytochemical compound of alkaloids, flavonoids, tannins, steroids and saponins, while the extract of roots contained all of compounds except saponins. Based on research can be concluded, that Enhalus acoroides also has antibacterial activity against Staphylococcus aureus that showed by the presence of inhibition zone. The present finding suggests that the extract of seagrass Enhalus acoroides can be used an antibacterial agent from marine.

scholarly journals An In Vitro Study on the Antimicrobial Properties of Essential Oil Modified Resin Composite against Oral Pathogens

Materials ◽  
2020 ◽  
Vol 13 (19) ◽  
pp. 4383
Author(s):  
Barbara Lapinska ◽  
Aleksandra Szram ◽  
Beata Zarzycka ◽  
Janina Grzegorczyk ◽  
Louis Hardan ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Antifungal Activity ◽  
Antimicrobial Agents ◽  
Resin Composite ◽  
Antimicrobial Properties ◽  
In Vitro Study ◽  
Diffusion Method ◽  
Oral Pathogens

Modifying the composition of dental restorative materials with antimicrobial agents might induce their antibacterial potential against cariogenic bacteria, e.g., S.mutans and L.acidophilus, as well as antifungal effect on C.albicans that are major oral pathogens. Essential oils (EOs) are widely known for antimicrobial activity and are successfully used in dental industry. The study aimed at evaluating antibacterial and antifungal activity of EOs and composite resin material (CR) modified with EO against oral pathogens. Ten EOs (i.e., anise, cinnamon, citronella, clove, geranium, lavender, limette, mint, rosemary thyme) were tested using agar diffusion method. Cinnamon and thyme EOs showed significantly highest antibacterial activity against S.mutans and L.acidophilus among all tested EOs. Anise and limette EOs showed no antibacterial activity against S.mutans. All tested EOs exhibited antifungal activity against C.albicans, whereas cinnamon EO showed significantly highest and limette EO significantly lowest activity. Next, 1, 2 or 5 µL of cinnamon EO was introduced into 2 g of CR and microbiologically tested. The modified CR showed higher antimicrobial activity in comparison to unmodified one. CR containing 2 µL of EO showed the best antimicrobial properties against S.mutans and C.albicans, while CR modified with 1 µL of EO showed the best antimicrobial properties against L.acidophilus.

Phytochemical analysis, Antimicrobial and Antioxidant assay of Bhut Jolokia pepper

2021 ◽  
pp. 3775-3778
Author(s):  
Dharani Dharan ◽  
K. Venkatesh ◽  
S.S. Meenambiga ◽  
Dhivya Dhanasekar ◽  
P. Arumugam
Keyword(s):  
Staphylococcus Aureus ◽  
Ethanolic Extract ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Zone Of Inhibition ◽  
Antioxidant Assay ◽  
Dpph Scavenging ◽  
Bhut Jolokia ◽  
Antimicrobial Assay

The presented work represents phytochemical analysis, Antioxidant assay and Antimicrobial activity of Bhut jolokia pepper (Capsicum chinense Jacq) extracted by ethanol as solvent. The ethanolic extract of Bhut jolokia pepper showed the presence of terpenoids, steroids, saponins and flavonoids. Antimicrobial assay was done with varying concentration (250-1000µg/ml) of pepper extract using tetracycline as control by well diffusion method, the extract at 750µg/ml shown best inhibition zone and Staphylococcus aureus showed the highest zone of inhibition at all concentration compared to other bacteria species with maximum zone of inhibition of 27mm. The DPPH scavenging assay for antioxidant activity at 517nm showed positive activity for scavenging, scavenging increased with the concentration of extract. Thus, Bhut jolokia could be effective in treating diseases caused by Staphylococcus aureus from antimicrobial assay result.

Sign in / Sign up

Export Citation Format

Share Document