scholarly journals Effect of Cercospora piaropi Tharp and Myrothecium roridum Tode Fries Formulated as Corn Oil Emulsion on Water Hyacinth Shoot Growth under Greenhouse Conditions

2021 ◽  
Vol 3 (1) ◽  
pp. 9-12
Author(s):  
Chrispo Mutebi ◽  
David M. Musyimi ◽  
George T. Opande

A study was done to find out the comparative effect of Cercospora piaropi Tharp and Myrothecium roridum Tode Fries formulated as corn oil emulsion on water hyacinth shoot growth and biomass under greenhouse conditions. The study site was located in Kibos at latitude 0037’S and longitude 37020’E with average temperature of 25 to 300C and 22 to 270C during the day and night respectively, and 60 to 69% relative humidity. Healthy water hyacinth plants were inoculated with the pathogens formulated in corn oil at 1x109, 1x108, 1x107, 1x106 and 1x105spores/ml.  The control plants were not inoculated. The experiment was set up in completely randomized design (CRD) with each treatment replicated three times. At weeks 2, 4, and 6 after inoculation, the average shoot length and biomass for the treated basins were separately compared to the average shoot length and biomass of the control plants. Increase in spore density for both pathogens significantly increased relative shoot length and relative biomass.  Relative shoot length was 55.07 and 51.93 for C. piaropi and M. roridum respectively at 1x109 spores/ml while relative biomass was 73.53 for C. piaropi and 37.60 at 1x109 spores/ml. Inoculation suppressed shoot elongation and biomass with 1x109 spores/ml being most effective. Cercospora piaropi formulated in corn oil lowered shoot length and biomass of water hyacinth more than M. roridum did.

HortScience ◽  
2016 ◽  
Vol 51 (7) ◽  
pp. 899-904 ◽  
Author(s):  
Fang Geng ◽  
Renae Moran ◽  
Michael Day ◽  
William Halteman ◽  
Donglin Zhang

These studies were conducted to determine the most effective methods for increasing shoot elongation during the initial proliferation stage of micropropagation in two dwarfing apple, Malus ×domestica (Borkh.), rootstock cultivars. Several experiments were conducted to compare explant collection date, exposure to chilling (5 ± 1 °C) temperatures, and varying concentrations of plant growth regulators in Murashige and Skoog (MS) media. Microshoot growth of ‘Geneva 41’ (‘G.41’) was very low and unaffected by chilling duration from 0 to 8 weeks or by gibberellic acid (GA3) concentration from 0 to 1.0 mg·L−1, but was improved by an additional subculture which increased shoot length from 1 to 15 mm. In ‘Geneva 30’ (‘G.30’), shoot elongation was most affected by date, chilling explants, and by optimizing cytokinin concentration and type. Explant collection date in April increased shoot growth compared with August or November. Microshoot growth of ‘G.30’ was increased by chilling nodal explants for 4 and 6 weeks when explants were collected in August and November, but not in April. Eight weeks chilling was detrimental for explants collected in April, and generally had little or no effect with August and November. The cytokinin 6-benzylaminopurine (BA) increased shoot number to a greater extent than thidiazuron (TDZ) or zeatin (ZT), and was also more effective for increasing shoot elongation with concentrations of 0 to 2.0 mg·L−1. In ‘G.30’, GA3 increased shoot growth at the optimum concentration of BA, but not with lower concentrations. ‘G.30’ microshoots were fewer and shorter with 24-epi-brassinolide (EBR) at concentrations of 0.1 and 1.0 mg·L−1. Chemical names: N-phenyl-N’-(1,2,3-thiadiazol-5-yl)urea (TDZ), 6-(4-hydroxy-3-methylbut-2-enylamino)purine (ZT).


AGRICA ◽  
2019 ◽  
Vol 12 (1) ◽  
pp. 43-58
Author(s):  
Wilhelmus Haman ◽  
Kristono Yohanes Fowo

This study aims to determine the response of the immersion time of vanilla stem cuttings in the most root growth regulating agent and to know the length of soaking time of the vanilla stem cuttings in the most optimum root growth regulator. The design used in this study was a Completely Randomized Design (CRD) with six treatments soaking in minutes. Repeated four times, namely P1 (10 minutes), P2 (20 minutes), P3 (30 minutes), P4 (40 minutes), P5 (50 minutes), P6 (60 minutes). Variables observed including the number of days of first shoot growth, the number of roots, root length, shoot diameter, shoot length, wet weight of cuttings, and dry weight of cuttings.The results showed that the treatment of P6 gave a very significant effect. On the variable growth in the number of roots (55.59%), root length (58.86%), number of days of first shoot growth (28 HST), shoot diameter (70.15%) , shoot length (71.43%), wet weight of cuttings (37.14%) and dry weight of cuttings (65.04%) at each age of observation.


2021 ◽  
Vol 3 (3) ◽  
pp. 81-84
Author(s):  
Chrispo Mutebi ◽  
George Opande

The effect of formulating Acremonium zonatum (Saw.) Gams in corn oil, mineral oil and glycerol was determined in a glasshouse at Maseno University Botanic Garden and laboratory. The study was situated at latitude -100’ 00’’ S and longitude 340 36’ 00’’E. A. zonatum was aseptically isolated from symptomatic plants and inoculated upon healthy water hyacinth plants in a CRD experiment.  Data was analyzed using SPSS version 20 computer software. Oil and spore density effects were statistically significant at p < 0.05. The main effect of oil yielded an effect size of 0.281 indicating that 28.1 % of the variance in disease severity was explained by the oil formulation (F(2, 28) = 5.459, p = 0.01). Spore density yielded an effect size of 0.326 indicating that 32.6% of the variance in the study was explained by spore density (F(3, 28) = 4.515, p = 0.011). The interaction effect was not significant (F(6, 28) = 0.199, p = 0.974) indicating that there was no combined effect for the kind of oil used in the formulation of the A. zonatum and the spore density. The results suggested that A. zonatum pathogenicity varies with different spore concentrations, 1x 108 spores/ml being the most potent. A. zonatum has favorable characteristics for consideration as a mycoherbicide. Corn oil as formulation material was concluded to be the best formulation material for A. zonatum.


Author(s):  
Abed Saad ◽  
Nour Abdurahman ◽  
Rosli Mohd Yunus

: In this study, the Sany-glass test was used to evaluate the performance of a new surfactant prepared from corn oil as a demulsifier for crude oil emulsions. Central composite design (CCD), based on the response surface methodology (RSM), was used to investigate the effect of four variables, including demulsifier dosage, water content, temperature, and pH, on the efficiency of water removal from the emulsion. As well, analysis of variance was applied to examine the precision of the CCD mathematical model. The results indicate that demulsifier dose and emulsion pH are two significant parameters determining demulsification. The maximum separation efficiency of 96% was attained at an alkaline pH and with 3500 ppm demulsifier. According to the RSM analysis, the optimal values for the input variables are 40% water content, 3500 ppm demulsifier, 60 °C, and pH 8.


2017 ◽  
Vol 17 (1) ◽  
pp. 22
Author(s):  
Arini Pradita Roselyn ◽  
Endang Linirin Widiastuti ◽  
G. Nugroho Susanto ◽  
Sutyarso '

Lung cancer is a disease that causes high mortality. Drugs used to prevent and cure cancer mostly causes intoxicity to the normal tissues due to its less effectiveness. Therefore, it is necessary to find out any agent or substance which works much more effective and safe for cancer treatment. The aim of the study was to elucidate the role of taurine on the lung tissue of mice (Mus musculus) induced by carcinogenic benzo(α)pyrene. The experiment was conducted in a completely randomized design with 5 replications. Six treatment groups were perfomed. Group I was given 0.2 mL of corn oil and given aquadest until the end of the study period, group II was induced by benzo(α)pyrene without administration of taurine, group III before induced with benzo(α)pyrene, was given taurine dosage 7.8 mg/BW/day for two weeks, group IV after induced benzo(α)pyrene, was given taurine with dosage 3.9 mg/BW/day, group V after induced benzo(α)pyrene, was given taurine with dosage7. 8 mg/BW/day, group VI after induced with benzo(α)pyrene, was given taurine with dosage 15.6 mg/BW/day. The results of the Kruskal-Wallis analysis and one way ANOVA with LSD (p>0,05) showed that taurine reduced lung tissue damage 72.73% due to the administration of benzo(α)pyrene of 0.3 mg/BW/day. In addition, the effective dose of taurine reduce lung tissue damage was 15.6 mg/BW/day.


2018 ◽  
Vol 12 (4) ◽  
pp. 379
Author(s):  
Adang Saputra ◽  
Lies Setijaningsih ◽  
Yosmaniar Yosmaniar ◽  
Tri Heru Prihadi

Permasalahan yang dihadapi pembudidaya ikan dengan sistem intensif adalah meningkatnya limbah yang terakumulasi pada air dan sedimen. Limbah budidaya ikan pada umumnya berupa padatan dan nutrien terlarut pada air terutama nitrogen dan fosfor. Tujuan penelitian ini adalah mengkaji distribusi nitrogen total dan fosfor total pada budidaya ikan gabus secara intensif yang diberi eceng gondok Eichhornia crassipes dan probiotik (Pseudomonas aeruginosa dan Achromobacter insuavis). Penelitian dirancang menggunakan rancangan acak lengkap dengan perlakuan pemberian kombinasi eceng gondok dan probiotik (A), pemberian eceng gondok (B), dan pemberian probiotik (C), masing-masing perlakuan diulang tiga kali. Benih ikan gabus yang digunakan berukuran panjang 14,74 ± 0,01 cm dan bobot 25,53 ± 0,09 g dengan padat tebar 175 ekor/kolam (50 ekor/m3). Selama 90 hari masa pemeliharaan, ikan gabus diberi pakan berupa pelet dengan kandungan protein sekitar 30%. Jumlah pemberian pakan 5% dari biomassa dengan frekuensi pemberian empat kali dalam sehari (pagi, siang, sore, dan malam). Hasil penelitian menunjukkan nitrogen dan fosfor pada budidaya ikan gabus terdistribusi pada eceng gondok, sedimen, air, dan ikan. Eceng gondok menyerap nitrogen dan fosfor paling tinggi (P<0,05) dibandingkan air, ikan, dan sedimen. Laju pertumbuhan spesifik bobot (4,37 ± 0,01%/hari) dan biomassa (1,88 ± 0,01 g) ikan gabus tertinggi dicapai pada pemberian kombinasi eceng gondok dan probiotik. Hasil ini dapat dijadikan landasan untuk pengelolaan limbah nitrogen dan fosfor pada budidaya ikan gabus secara intensif.One of the problems in intensive aquaculture system is the the accumulation of waste in the water and sediment. Aquaculture wastes are discharged into the water in form of solids and dissolved nutrients which mostly consisted of nitrogen and phosphorus. The purpose of this study was to study the dynamics of total nitrogen and phosphorus in an intensive aquaculture media supplied with water hyacinth and probiotics (Pseudomonas aeruginosa and Achromobacter insuavis). The study was designed using a completely randomized design with treatment combinations of water hyacinth with probiotic (A), water hyacinth (B), and probiotic (C). Each treatment consisted of three replications. The seeds of snakehead used had body length of 14.74 ± 0.01 cm and weight 25.53 ± 0.09 g, stocked in ponds with stocking density of 175 individuals/pond (50 individuals/m3). During 90 days of rearing, the fish were fed with pellet with protein content of 30%. The amount of feeding was 5% of the biomass with feeding frequency of four times a day (morning, afternoon, evening, and night). The results showed that the produced nitrogen and phosphorus in the snakehead cultivation were distributed to water hyacinth, sediment, water, and fish. Water hyacinth absorbed most of the nitrogen and phosphorus compared to water, fish, and sediment. Higher specific growth rate (4.37 ± 0.01%/day) and biomass (1.88 ± 0.01 g) of snakehead were achieved in combination of water hyacinth and probiotic treatment. These results can be used as a basis for the management of nitrogen and phosphorus wastes in an intensive fish farming.


1981 ◽  
Vol 11 (4) ◽  
pp. 789-795 ◽  
Author(s):  
S. Thompson

When seedlings of a single seed source of Scots pine (Pinussylvestris L.) were raised for 26 weeks in a naturally lit, heated greenhouse, two types of shoot morphology were observed. Type 1 was that normally found in 1-year-old seedlings. Type 2 had a shoot morphology similar to that of seedlings raised outdoors for two growing seasons. When compared with type 1 plants, type 2 plants had an earlier start to shoot elongation, set their buds earlier, and stopped shoot elongation sooner. After one growing season, type 2 plants were shorter, had fewer stem units for shoot elongation in the second season, but carried a greater foliage biomass than 1-year-old type plants. After two seasons they remained shorter. Thus, plant rearing practices which result in the production of seedlings with this type of shoot morphology arc undesirable.The relationship between early "budsct," shoot morphology, and plant height suggests that the proportion of seedlings with a 2-year-old shoot morphology after one growing season in a heated greenhouse may be used as an early test for height growth potential in seed origins and possibly in progenies of north temperate pine species.


Author(s):  
FLORENDA C. BALLESTEROS-TEMANEL

Brassinosteroids (BRs) are new class of hormones noted to perform multiplephysiological functions in plant growth and development and have the potentialof influencing cell and tissue growth in vitro. Many naturally occurring BRs,including brassinolide, have been discovered, their mode of action and their growthpromoting activities on plants. The use of brassinolide in in vitro propagation isnew. The Murashige and Skoog (1962) medium was used as basal medium. Plantgrowth regulators - IAA, BA and BR - were added to the medium. The study usedthe Completely Randomized Design (CRD) in factorial with three replications.The cultivar of banana and plant growth substances affected the number of budsproduced, shoot length, root length, and stem girth. The interaction of thesetwo factors (cultivar x PGR) influenced the number of buds produced in vitroand the shoot length of the meriplants. The study shows that brassinolide has aninfluence on shoot induction, proliferation, and elongation of bananas in in vitro propagation.Keywords: Agriculture, in vitro propagation, induction, proliferation, elongation, apical meristem, plant growth regulators, cultivars, Isabela, Philippines


2004 ◽  
Vol 44 (3) ◽  
pp. 353 ◽  
Author(s):  
R. S. Tegg ◽  
P. A. Lane

The increased use of semi and fully enclosed sports stadiums necessitates the ongoing selection, development and assessment of shade-tolerance in turfgrass species. Vertical shoot growth rate is a simple biological measure that may supplement visual turfgrass assessment and provide a useful measure of shade adaptation. Cool-season temperate turfgrasses; Kentucky bluegrass–perennial ryegrass (Poa pratensis L.–Lolium perenne L.), creeping bentgrass (Agrostis palustris Huds.), supina bluegrass (Poa supina Schrad.) and tall fescue (Festuca arundinacea Schreb.), and a warm season species, Bermudagrass (Cynodon dactylon L.), were established in pot and field experiments and subjected to 4 shade treatments (0, 26, 56 or 65% shade) under ambient conditions. Average light readings taken near the winter and summer solstice in full sunlight at midday, were 790 and 1980�μmol/m2.s, respectively. Field and pot trials confirmed supina bluegrass and tall fescue to have the greatest shade tolerance, producing high turf quality under 56 and 65% shade. However, all turfgrass species declined in quality under high shade levels as indicated by an increase in thin, succulent vertical growth, and a less-dense turf sward. Vertical shoot growth rates of all species increased linearly with increasing shade levels. Kentucky bluegrass–perennial ryegrass had the highest rate of increase in vertical shoot elongation under shade, approximately 3.5 times greater than supina bluegrass, which had the lowest. Low rates of increase in vertical shoot elongation under shade indicated shade tolerance whereas high rates inferred shade intolerance.


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