scholarly journals OCCURRENCE OF AFLATOXIN M1 IN RAW ANIMAL MILK AND DAIRY PRODUCTS IN NORTHERN NIGERIA

2021 ◽  
Vol 4 (2) ◽  
pp. 12-15
Author(s):  
Akinyemi Oluwatosin ◽  
Daniella Ekpakpale ◽  
Oluwawapelumi Oyedele ◽  
Kolawole Ayeni ◽  
Stephen O. Fapohunda ◽  
...  
Keyword(s):  
Goat Milk ◽  
Camel Milk ◽  
Cow Milk ◽  
Assay Method ◽  
Aflatoxin M1 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Northern Nigeria ◽  
The European Union ◽  
Mean Values ◽  
Animal Milk

A total of 144 samples of milk and milk products comprising 23 raw camel milk, 77 cow milk, 24 goat milk and 20 kindirmo (fermented milk) were randomly purchased across four states in northern Nigeria during July 2020 and screened for aflatoxin M1 (AFM1) using an Enzyme-linked immunosorbent assay method. The incidence (and mean values) of AFM1 in the camel milk, cow milk, goat milk and kindirmo samples were 74 % (38 ng/L), 99 % (92 ng/L), 100 % (112 ng/L) and 100 % (145 ng/L), respectively. The mean AFM1 levels in 22 %, 42 %, 83 % and 50 % of the camel milk, cow milk, goat milk and kindirmo samples, respectively, exceeded the European Union threshold of 50 ng/L. Results from this study suggest that consumption of raw animal milk and its products could be a contributing factor to aflatoxin exposure among households in northern Nigeria.

scholarly journals Role of Cardiac Biomarkers in the Assessment of Acute Cerebrovascular Accident

2021 ◽  
Vol 12 (01) ◽  
pp. 106-111
Author(s):  
Srinivasan Radhakrishnan ◽  
Swathy Moorthy ◽  
Sudish Gadde ◽  
Krishnaswamy Madhavan
Keyword(s):  
Acute Stroke ◽  
Cerebrovascular Accident ◽  
Troponin I ◽  
Serum Levels ◽  
Assay Method ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cardiac Enzymes ◽  
Mean Values ◽  
Creatine Kinase Mb ◽  
The Mean

Abstract Background Stroke (cerebrovascular accident) has for long been a global burden in terms of its morbidity and mortality. Serum levels of cardiac enzymes such as creatine kinase-MB (CK-MB) component, troponin T, and brain natriuretic peptide have been found to be elevated among the patients with stroke and also serve to prognosticate these patients. The serum levels of these enzymes correlate directly to the severity of stroke in these patients. Objective Elevated cardiac enzymes among patients with acute cerebrovascular accidents are not uncommon despite the patients not having any cardiac problems. We aimed to identify the occurrence of elevated cardiac enzymes among patients with acute stroke and their correlation with the severity of stroke. Materials and Methods Our study included 100 patients of acute stroke with no previous history of cardiac ailments. Serum levels of troponin I and CK-MB were analyzed among these patients using enzyme-linked immunosorbent assay method within the first 2 hours of admission. Patients outcome during the hospital stay were analyzed. Stroke severity was assessed using the National Institute of Health Stroke score (NIHS score) and the modified Rankin Score (mRS). The cardiac enzyme levels were correlated with these scores. Results Twenty-eight percent of patients had elevated troponin I, while 72% patients had normal levels with the mean values of 10.36 to 106.54 ng/mL and 0.00 to 0.02 ng/mL, respectively. CK-MB levels were found elevated among 14% patients and normal among 86% patients with mean values of 5.8 to 124.36 and 0.0 to 4.3 ng/mL, respectively. Among the six patients who succumbed to death, three patients had increased troponin I and four had elevated CK-MB. NIHS scores of 21.0357±6.79 and 105.277±5.564 were seen in patients with elevated and normal troponin I, whereas NIHS scores of 20.4285±8.658 and 11.8721±9.273 were seen among patients with increased and normal CK-MB, respectively. The mRS scores were 4.3214±0.367, 2.4305±1.374, 4.2143±1.412, and 2.756±1.749 ng/mL among the patients with elevated and normal troponin I and CK-MB, respectively. Conclusion The mean values of cardiac enzymes troponin I and CK-MB were higher among patients with higher scores of NIHS and mRS. Among them, troponin I was very significant and it may serve as an early biomarker for the severity of stroke and hint on early cardiac evaluation among these patients.

scholarly journals Growth and enterotoxin production of Bacillus cereus in cow, goat, and sheep milk

2014 ◽  
Vol 83 (10) ◽  
pp. S3-S8 ◽  
Author(s):  
Lenka Necidová ◽  
Šárka Bursová ◽  
Alena Skočková ◽  
Bohdana Janštová ◽  
Pavla Prachařová ◽  
...  
Keyword(s):  
Bacillus Cereus ◽  
Storage Temperature ◽  
Goat Milk ◽  
Storage Conditions ◽  
Cow Milk ◽  
Enzyme Linked Immunosorbent Assay ◽  
Plate Method ◽  
Milk Samples ◽  
Sheep Milk ◽  
Enterotoxin Production

The aim of this study was to compare Bacillus cereus growth rates and diarrhoeal enterotoxin production in raw and pasteurized goat, sheep, and cow milk in terms of storage conditions. Milk samples were inoculated with B. cereus (CCM 2010), which produces diarrhoeal enterotoxins. Enterotoxin production was tested by ELISA (Enzyme-Linked Immunosorbent Assay), and the count of B. cereus was determined by the plate method. With raw cow milk, B. cereus growth and enterotoxin production can be completely suppressed; in raw goat and sheep milk, enterotoxin was produced at 22 °C. In pasteurized cow, goat, and sheep milk, the B. cereus count increased under all storage conditions, with more rapid growth being observed at 15 °C (sheep milk) and 22 °C (cow and goat milk). Enterotoxin presence was detected at 15 °C and 22 °C, and with pasteurized cow milk also at 8 °C. Our model experiments have determined that B. cereus multiplication and subsequent enterotoxin production depend on storage temperature and milk type.

scholarly journals Camel Milk Is a Safer Choice than Goat Milk for Feeding Children with Cow Milk Allergy

ISRN Allergy ◽  
2011 ◽  
Vol 2011 ◽  
pp. 1-5 ◽  
Author(s):  
Mohammad Ehlayel ◽  
Abdulbari Bener ◽  
Khalid Abu Hazeima ◽  
Fatima Al-Mesaifri
Keyword(s):  
Atopic Dermatitis ◽  
Prospective Study ◽  
Goat Milk ◽  
Specific Ige ◽  
Cross Reactivity ◽  
Medical Examination ◽  
Camel Milk ◽  
Cow Milk ◽  
Milk Allergy ◽  
Cow Milk Allergy

Background. Various sources of mammalian milk have been tried in CMA. Objectives. To determine whether camel milk is safer than goat milk in CMA. Methods. Prospective study conducted at Hamad Medical Corporation between April 2007 and April 2010, on children with CMA. Each child had medical examination, CBC, total IgE, cow milk-specific IgE and SPT. CMA children were tested against fresh camel and goat milks. Results. Of 38 children (median age 21.5 months), 21 (55.3%) presented with urticaria, 17 (39.5%) atopic dermatitis, 10 (26.3%) anaphylaxis. WBC was 10,039±4,735 cells/μL, eosinophil 1,143±2,213 cells/μL, IgE 694±921 IU/mL, cow's milk-specific-IgE 23.5±35.6 KU/L. Only 7 children (18.4%) tested positive to camel milk and 24 (63.2%) to goat milk. 6 (15.8%) were positive to camel, goat, and cow milks. Patients with negative SPT tolerated well camel and goat milks. Conclusions. In CMA, SPT indicates low cross-reactivity between camel milk and cow milk, and camel milk is a safer alternative than goat milk.

Aflatoxin M1 in Milk Products in China and Dietary Risk Assessment

2013 ◽  
Vol 76 (5) ◽  
pp. 849-853 ◽  
Author(s):  
YAODONG GUO ◽  
YAHONG YUAN ◽  
TIANLI YUE
Keyword(s):  
Body Weight ◽  
Cancer Risk ◽  
Probabilistic Approach ◽  
Food Additives ◽  
World Health ◽  
Assay Method ◽  
Aflatoxin M1 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Expert Committee ◽  
Milk Products

This study was conducted to investigate the occurrence of aflatoxin M1 (AFM1) in milk products in China using the competitive enzyme-linked immunosorbent assay method and to estimate the dietary exposure to this toxin through a probabilistic approach. Based on the exposure assessment results, a quantitative cancer potency formula developed by the Joint Food and Agriculture Organization and World Health Organization Expert Committee on Food Additives was applied to assess the cancer risk. AFM1 was detected in 48.07% of the milk samples and 4.49% of the yoghurt samples. No samples contained AFM1 above the current regulatory limit in China. The simulated AFM1 intake (90% confidence interval) in various sex-age groups ranged from 0.023 (0.021 to 0.023) ng/kg of body weight per day for 30- to 45-year-old men to 0.382 (0.354 to 0.386) ng/kg of body weight per day for 2- to 4-year-old girls at the 99th percentile. The cancer risk of AFM1 to the general population of China was assessed to be 0.129 cancer cases per year per 108 persons at the 99th percentile. These results indicate that the health risk associated with AFM1 in milk in China is relatively low.

scholarly journals Occurrence and seasonal variation of aflatoxin M1 in raw cow milk collected from different regions of Algeria

2020 ◽  
Vol 13 (3) ◽  
pp. 433-439 ◽  
Author(s):  
Sarah Mohammedi-Ameur ◽  
Mohammedi Dahmane ◽  
Carlo Brera ◽  
Moustafa Kardjadj ◽  
Meriem Hind Ben-Mahdi
Keyword(s):  
Animal Feed ◽  
Control Program ◽  
Raw Milk ◽  
Aflatoxin Contamination ◽  
Cow Milk ◽  
Aflatoxin M1 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Survey Period ◽  
Lactating Cows ◽  
The Usa

Background and Aim: Aflatoxins are metabolites of molds that exert potentially toxic effect on animals and humans. This study aimed to investigate the occurrence of aflatoxin M1 (AFM1) in raw cow milk collected during 1 year (2016-2017) from different regions of Algeria and risk factors associated with the contamination. Materials and Methods: During the survey period, 84 samples of raw milk were collected in three regions of Algeria (northeast, north center, and northwest) during four seasons. AFM1 levels were analyzed by competitive enzyme-linked immunosorbent assay. Results: AFM1 was detected in 39 (46.43%) samples (total mean concentration, 71.92 ng/L; range, 95.59-557.22 ng/L). However, the AFM1 levels exceeded the maximum tolerance limit set by the Food and Drug Administration in the USA (500 ng/L) in only 1 sample (1.19%). Statistical analysis revealed significant differences (p<0.005) between AFM1 levels in milk samples collected in the spring and autumn. The mean AFM1 levels in samples collected in the spring were significantly higher than those in samples collected in autumn. Conclusion: The survey indicates that farmers involved in milk production should be made aware of the adverse effects of aflatoxin contamination in animal feed. A systematic control program of supplementary feedstuff for lactating cows should be introduced by the public health authorities.

A Study on the Presence of Aflatoxin M1 in Cow’s Milk in Jiroft

2021 ◽  
Author(s):  
Mohadeseh Kamali ◽  
Seyyedeh Sedigheh Seyyedi ◽  
Mehdi Taheri Sarvtin
Keyword(s):  
Immune Deficiency ◽  
Chemical Form ◽  
Aflatoxin M1 ◽  
Cow’S Milk ◽  
Enzyme Linked Immunosorbent Assay ◽  
The European Union ◽  
Cow's Milk ◽  
Tolerance Level ◽  
Milk Samples ◽  
Maximum Tolerance

Background and Aims: Cow's milk is a daily staple food for many individuals that can be contaminated with many toxins such as aflatoxin M1 (AFM1). AFM1 is a chemical form of the aflatoxin B1 produced by some species of Aspergillus genus like A. ochraceus, A. flavus, A. nomius, and A. parasiticus that can contaminate feed and forage cattle. This toxin enters into the milk after eating contaminated feed by cows. AFM1 can cause various dangerous diseases such as cancer and immune deficiency in humans. The present study is aimed to investigate the level of AFM1 in cow's milk in Jiroft, Kerman Province, Iran. Materials and Methods: A total of 90 cow’s milk samples were collected in spring and summer 2019 from available stores in Jiroft city. Enzyme-linked immunosorbent assay was used to measure AFM1 in all cow’s milk samples.Results: In the present study, AFM1 was found in 88 (97.8%) milk samples with a range of 0.2-90.62 ppt (mean, 20.07±24.46 ppt). AFM1 concentrations exceeded 50 ppt (maximum tolerance level of AFM1 in the European Union) was seen in 12 (13.3%) samples. Conclusions: The results of this study showed the presence of AFM1 in cow's milk in Jiroft city. So, in this region, many people are exposed to dangerous diseases such as cancer due to the consumption of milk contaminated with AFM1.

CONTROL PROBLEMS AND METHODS FOR DETECTING RESIDUAL AMOUNTS OF ANTIBACTERIAL SUBSTANCES IN MILK AND DAIRY PRODUCTS

2020 ◽  
Vol 1 (2) ◽  
pp. 165-169
Author(s):  
G.M. Goryainova ◽  
◽  
E.A. Denisova ◽  
L.V. Arsen`eva ◽  
V.S. Babunova ◽  
...  
Keyword(s):  
Dairy Products ◽  
High Performance ◽  
Raw Milk ◽  
Detection Methods ◽  
Assay Method ◽  
Enzyme Linked Immunosorbent Assay ◽  
Official Method ◽  
The European Union ◽  
Residual Amount ◽  
Milk And Dairy Products

The article is devoted to the study of control issues and methods for detecting residual amounts of antibacterial substances in milk and dairy products. The presence of a large number of antibacterial drugs on the Russian market and their use in dairy farming makes it an urgent problem to detect their residual amount, both in raw milk and in dairy products. The lack of standardized detection methods, sufficiently equipped and accredited laboratories, and the high cost and duration of research are obstacles to monitoringthe content of the residual amount of antibiotics., including state control. Currently, the state monitoring system for screening antibiotics uses the enzyme-linked immunosorbent assay method (ELISA), which is the official method for monitoring animal products adopted in the European Union. To confirm the quantitative content of antibiotics, a high-performance liquid chromatography (HPLC) method with various types of detectors is used, which is reliable but very time-consuming for screening a large number of samples. Using the patented Randox Biochip technology as a method for detecting the residual amount of antibiotics in milk and dairy products, it would be possible to determine up to 25 antibacterial substances in one sample, including those that are not often used in veterinary practice.

IDEXX SNAP® Beta-Lactam ST Validation for Penicillin G Detection

2013 ◽  
Vol 96 (6) ◽  
pp. 1343-1349 ◽  
Author(s):  
Travis T Waldron
Keyword(s):  
Goat Milk ◽  
Rapid Test ◽  
Cow Milk ◽  
Penicillin G ◽  
The European Union ◽  
Beta Lactam ◽  
Powdered Milk ◽  
Sheep Milk ◽  
Test Kit ◽  
Assay Time

Abstract IDEXX has produced a robust and improved rapid test kit optimized to detect penicillin G in a variety of milk matrixes. The SNAP® Beta-Lactam ST Test Kit is designed to be run without the use of a heat block. The new test is optimized to ensure a detection capability for penicillin G that is at or below the European Union maximum residue limit of 4 parts per billion. The test can be used with commingled cow milk, commingled goat milk, commingled sheep milk, and reconstituted whole fat powdered milk. The SNAP Beta-Lactam ST Test Kit contains all the items necessary to run and interpret the test in a single package. No heat block or reader is required. The results can be read visually or with an IDEXX SNAPshot or SNAPshot DSR Reader. The total assay time is approximately 7 minutes.

scholarly journals Assessment of Aflatoxin M1 in human breast milk in Rafsanjan, Iran

2021 ◽  
Author(s):  
Somayeh Pourtalebi ◽  
Seyyed Amin Ayatollahi Mousavi ◽  
Zahra Assadollahi ◽  
Seyyed Mahdi Mousavi
Keyword(s):  
Breast Milk ◽  
Cross Sectional Study ◽  
Aflatoxin M1 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Human Breast Milk ◽  
The European Union ◽  
Postnatal Exposure ◽  
Cross Sectional ◽  
Milk Samples ◽  
Lactating Mothers

Background and Purpose: Aflatoxins (AFs) are a group of highly toxic mycotoxins present both in the environment and in foodstuffs. The food of infants should be safe and free of various pollutants, including breast milk mycotoxins. This study aimed to measure the mycotoxin of Aflatoxin M1 (AFM1) in human milk samples obtained from lactating mothers living in Rafsanjan city, Iran.  Materials and Methods: In the current cross-sectional study, breast milk samples were collected from 150 lactating mothers in Rafsanjan city from September 2015 to April 2016 using the structured food-frequency questionnaire. The AFM1 was measured by employing enzyme-linked immunosorbent assay specific kits. The statistical analysis was performed in SPSS software (version 16). Results: The AFM1 was detected in 98 mothers (65%) with a mean concentration of 14.69±8.15 ng/kg, ranging from 5.02 to 41.25 ng/kg. The AFM1 concentration exceeded the tolerable and accepted limit promulgated by the European Union and the USA (25 ng/kg) in only 10 milk samples. Moreover, in 59 milk samples, the AFM1 concentration exceeded the limit recommended by Australia and Switzerland (10 ng/kg). Conclusion: According to the results of the present study, lactating mothers and their infants are at risk of AFM1 exposure in southern Iran. Accordingly, the examination of AFM1 concentrations in lactating mothers, as a critical postnatal exposure marker of infants to this carcinogenic compound, requires further studies in various seasonal periods and different parts of Iran.

Rapid detection of cow milk adulteration/contamination in goat milk by a lateral flow colloidal gold immunoassay strip

2019 ◽  
Vol 86 (1) ◽  
pp. 94-97 ◽  
Author(s):  
Bochao Liu ◽  
Jinhong Si ◽  
Fang Zhao ◽  
Qi Wang ◽  
Yu Wang ◽  
...  
Keyword(s):  
Colloidal Gold ◽  
Rapid Detection ◽  
Goat Milk ◽  
Lateral Flow ◽  
Cow Milk ◽  
Quantitative Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Milk Adulteration ◽  
Milk Casein ◽  
Higher Sensitivity

AbstractCurrent available methods to detect cow milk adulteration or accidental contamination of goat milk are both laborious and time consuming. The aim of this technical research communication was to develop a simple, rapid, specific and sensitive method for quantitative detection of cow milk in goat milk. A competitive lateral flow immunoassay (LFIA) strip was developed using a specific monoclonal antibody (mAb) labeled with colloidal gold nanoparticles (GNPs) for specifically binding to cow milk casein. The detection limit of this rapid detection was 0.07% of cow milk in goat milk, providing equal specificity and higher sensitivity when compared with a commercial enzyme-linked immunosorbent assay (ELISA). These result suggest that the established rapid GNPs-LFIA strip could be used for monitoring cow milk adulteration/contamination of goat milk.

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