STRUCTURAL AND FUNCTIONAL STATE OF THE SEMINAL GLANDSIN THE DYNAMICS OF ACUTE INFECTIOUS INFLAMMATION

2019 ◽  
Vol 72 (8) ◽  
pp. 1486-1490
Author(s):  
Olga I. Zalyubovska ◽  
Tetiana I. Tiupka ◽  
Victor V. Zlenko ◽  
Yulia N. Avidzba ◽  
Mycola I. Lytvynenko ◽  
...  
Keyword(s):  
Sertoli Cells ◽  
Morphological Changes ◽  
Experimental Studies ◽  
Sharp Decrease ◽  
Type A ◽  
Male Rats ◽  
Reserve Capacity ◽  
Type B ◽  
Adaptive Processes ◽  
Spermatogenic Epithelium

Introduction: Negative demographic trends are often associated with high levels of infertility, including male. In the modern literature there are data on morphofunctional changes in various organs and tissues during inflammation of various origins, obtained in experiments on animals. At the same time, there are practically no studies on changes in the seminal glands in inflammation of different etiologies. The aim of this study is to identify the morphofunctional features of the seminal glands in the dynamics of acute infectious (staphylococcal) inflammation in rats. Materials and methods: Experimental studies were performed on 40 nonlinear male rats weighing 180-200 g. Microscopic and histochemical studies were performed on the 7th, 14th, and 28th day. Results: On the 7th day of staphylococcal inflammation, morphofunctional changes in the seminal glands were detected in rats in the form of a moderate rearrangement of the spermatogenic epithelium, which was manifested by a decrease in Sertoli cells and the number of type A light spermatogonia along with an increase in the number of type A dark spermatogonia and type B spermatogonia. The described changes were accompanied by a decrease in the metabolism of nucleoproteins in epithelial cells. On the 14th day, the morphological changes were characterized by a sharp decrease in Sertoli cells, the absence of type A light spermatogonia and an increase in the number of type A dark spermatogonia and type B spermatogonia. After 28 days, there is an increase in the number of tubules with the presence of type A light and dark spermatogonia, as well as single Sertoli cells, which indicates the restoration of the morphofunctional state of the seminal glands. Conclusions: More pronounced compensatory-adaptive processes in the seminal glands occur within a period of 28 days from the start of modeling of staphylococcal inflammation. The latter is confirmed by the appearance of various shapes and sizes of tubules with restored spermatogenic epithelium of various stages of development. The presence of type A light and dark spermatogonia indicates the reserve capacity of the seminal glands.

scholarly journals Cloning, pharmacological characterization, and expression analysis of Atlantic salmon (Salmo salar L.) nuclear progesterone receptor

Reproduction ◽  
2011 ◽  
Vol 141 (4) ◽  
pp. 491-500 ◽  
Author(s):  
Shi X Chen ◽  
Jan Bogerd ◽  
Eva Andersson ◽  
Fernanda F L Almeida ◽  
Geir Lasse Taranger ◽  
...  
Keyword(s):  
Progesterone Receptor ◽  
Atlantic Salmon ◽  
Sertoli Cells ◽  
Plasma Levels ◽  
Type A ◽  
Mrna Levels ◽  
Early Type ◽  
Type B ◽  
Late Type ◽  
Nuclear Progesterone Receptor

To better understand the role(s) of progestogens during early stages of spermatogenesis, we carried out studies on the nuclear progesterone receptor (Pgr) of the Atlantic salmon. Its open-reading frame shows the highest similarity with other piscine Pgr proteins. When expressed in mammalian cells, salmon Pgr exhibited progestogen-specific, dose-dependent induction of reporter gene expression, with 17α,20β-dihydroxy-4-pregnen-3-one (DHP) showing the highest potency. We then analyzed testicular pgr mRNA and DHP plasma levels in animals during the onset of spermatogenesis, which were exposed to natural light or to constant light, to induce significant differences in testis growth. Grouping of the animals according to their progress through spermatogenesis showed that testicular pgr mRNA levels as well as DHP plasma levels first increased when germ cells had reached the stage of late type B spermatogonia and further increased when entered meiosis, i.e. when spermatocytes were present. However, in situ hybridization studies revealed that pgr mRNA expression was restricted to Sertoli cells, with a strong signal in Sertoli cells contacting type A/early type B spermatogonia, while Sertoli cells contacting larger germ cell clones with further differentiated stages (e.g. late type B spermatogonia) were less intensely/not stained. We conclude that the increase in pgr mRNA levels per pair of testis reflects, at least in part, the increased number of Sertoli cells enveloping type A and early type B spermatogonia. We propose that Sertoli cell-expressed Pgr may mediate DHP-stimulated early steps in spermatogenesis in Atlantic salmon, such as an increase in the number of new spermatogonial cysts.

scholarly journals 315 AN ATTEMPT AT INDUCING DIFFERENTIATION INTO ROUND SPERMATIDS OF RAT SPERMATOGONIA BY CO-CULTURING WITH SERTOLI CELLS

2005 ◽  
Vol 17 (2) ◽  
pp. 308 ◽  
Author(s):  
Y. Iwanami ◽  
T. Kobayashi ◽  
M. Kato ◽  
M. Hirabayashi ◽  
S. Hochi
Keyword(s):  
Cell Population ◽  
Sertoli Cells ◽  
Cultured Cells ◽  
Uv Light ◽  
Close Association ◽  
Type A ◽  
Round Spermatid ◽  
Full Term ◽  
Male Rats ◽  
Seminiferous Tubules

Mammalian spermatogenesis is a complex process of germ cell development at the seminiferous tubules whereby diploid spermatogonia proliferate and differentiate into haploid spermatozoa via round and elongating spermatids in close association with somatic Sertoli cells. In the present study, the potential of rat spermatogonia to undergo meiosis during co-culture with Sertoli cells was assessed. The type-A spermatogonia and Sertoli cells were prepared from Day 7 heterozygous transgenic male rats carrying EGFP DNA, and co-cultured on the dishes (coated; BD Falcon™ 35-3801, or non-coated: BD Falcon™ 35-1008, 4 × 106 cells/4-mL dish) at 37°C for 3 days and at 34°C for a subsequent 7 days in 5% CO2 in air. The culture medium was DMEM medium supplemented with 10% fetal bovine serum, growth factors (10 ng/mL EGF and 10 ng/mL IGF) and various hormones (500 ng/mL FSH, 133 μIU/mL hGH, 5 μg/mL insulin, 0.1 μM testosterone and 0.1 μM dihydrotestosterone). During culture, appearance of round spermatid-like cells (ca. 15 μm in cellular diameter and 7–8 μm in nuclear diameter) was traced. The ploidy of the cells was also analyzed by flow cytometry (FCM). At the end of culture, the proportion of EGFP DNA-bearing cells in the total cultured cell population was examined under UV light at 365 nm. Thereafter, continuation of the spermatid-like cells to full-term development was examined by ooplasmic microinjection (Kato et al. 2004 Contemp. Top. Lab. Anim. Sci. 43/2, 13). Briefly, oocytes from the Sprague-Dawley rats were denuded, activated with two direct-current pulses at 100 V/mm for 99 μs and held in 2 mM 6-dimethylaminopurine for 20 min. The nuclei of spermatid-like cells were microinjected into the oocytes by using a piezo impact driving unit, and the injected oocytes after 24 h culture were transferred into recipients. Round spermatid-like cells were first observed at the 5th day of culture on both dishes, but the proportion of spermatid-like cells on the coated dish was higher than that on the non-coated dish. The FCM analysis showed that a single peak of haploid cells was detected in the cell population cultured on the coated dish at the 5th day of culture, while no haploid peak was detected on the non-coated dish. The cultured cells exhibited two distinct patterns of EGFP fluorescence, with a proportion of EGFP-positive cells at 53.5% (total 1,000 counts). The microinsemination into 263 oocytes resulted in the production of 27 oocytes with two pronuclei (10.3%) and 15 cleaved oocytes (5.7%). However, the oviductal transfer of 143 microinseminated oocytes resulted in only 8 implantation sites without viable offspring (5.6%). These results indicated that rat type-A spermatogonial cells seemed to undergo meiosis, but the potential of the cultured spermatid-like cells to participate into full-term development was questionable.

The population of germ cells in immature male rats following irradiation at birth

1966 ◽  
Vol 165 (998) ◽  
pp. 103-135 ◽  
Keyword(s):  
Germ Cells ◽  
Total Population ◽  
Primordial Germ Cells ◽  
Type A ◽  
Male Rats ◽  
Type B ◽  
The Absolute ◽  
The Difference ◽  
Transitional Cells ◽  
Type A Spermatogonia

Male rats were irradiated with 19 r on the day of birth, and killed at intervals ranging from 5 to 18 days. Estimates were made of the absolute and relative numbers of germ cells at different stages of spermatogenesis in 64 irradiated and 61 untreated specimens. In the normal rat, the calculated population of germ cells increased from about 160000 at 5 days to 30 million at 18 days. Only negligible numbers of primordial germ cells (gonocytes and transitional cells) persisted beyond the age of 10 days. Small numbers of spermatogonia type A appeared at 5 days (15000) and their population rose to about 1 million at 12 days, and 2 million at 18 days (7 % of all germ cells). Intermediate spermatogonia first occurred in appreciable numbers (23000 to 55000) at 8 or 9 days, when the population of type-A spermatogonia was 360000. The subsequent rise in the population of intermediate spermatogonia was more rapid than that of type A (4 million at 18 days). Spermatogonia type B and primary spermatocytes appeared at 9 to 10 days, and their numbers rose more steeply still (6.5 and 16 million at 18 days, respectively). Irradiation at birth exerted no rapid effect on the cytological appearance of primordial germ cells. Transformation from gonocytes to transitional cells appeared to proceed normally and the estimated total population of germ cells at 5 days was no smaller than in the controls. Subsequently, however, many of the transitional cells failed to divide: they enlarged to form giant cells, acquired bizarre nuclear outlines, and persisted for unusually long periods. Some degenerated at mitotic prophase or metaphase, while a few seemed to die at interphase, without entering division. The calculated total population of germ cells in irradiated rats rose from 160000 at 5 days to 9.4 million at 18 days. Small numbers of spermatogonia type A, presumably derived from such primordial germ cells as were able to complete mitosis, appeared some 2 to 3 days later than in controls. The number of type-A spermatogonia in 7-day-old irradiated rats was 44000, cf. 215000 in controls; the difference became less pronounced with time, and by the age of 18 days, the population of 1.9 million was comparable to that estimated for the controls. Small numbers of intermediate spermatogonia appeared on the 9th (8000) and 10th day (35000), when the population of type-A spermatogonia was about 110000 and 260000 respectively. By the 18th day, intermediate spermatogonia numbered 2 million. The populations of type-B spermatogonia and primary spermatocytes rose from 11000 to 13000 at 10 days to 1.6 and 3.4 million, respectively, at 18 days. The difference in the absolute and relative numbers of germ cells between normal and irradiated testes widened progressively with advance in the developmental stage of the germ cells. Analysis of the results indicates that in the reduced population of spermatogonia type A after irradiation, the pattern of spermatogonial mitoses is modified so as to favour the formation of more type-A, in preference to intermediate, spermatogonia.

scholarly journals ALTERATION OF BRAIN TISSUE, LIVER, AND KIDNEY IN THE POST-CONTACT PERIOD IN WHITE RATS EXPOSED TO VIBRATION

2019 ◽  
Vol 98 (10) ◽  
pp. 1108-1112
Author(s):  
Eugeny A. Titov ◽  
V. A. Pankov ◽  
A. V. Lizarev ◽  
M. V. Kuleshova
Keyword(s):  
Brain Tissue ◽  
Liver Tissue ◽  
Morphological Changes ◽  
White Male ◽  
Experimental Studies ◽  
Kidney Tissue ◽  
Male Rats ◽  
Ultrastructural Changes ◽  
Contact Period ◽  
White Rats

Introduction. Experimental studies in animals have shown ultrastructural changes in hepatic sinusoidal endothelial cells, tissue hypoxia of the kidneys, changes in the activity of oxidative processes and antioxidant enzymes, the formation of bioenergetic hypoxia, cell response in the form of infiltration of both lymphoid and macrophage cells to develop due to exposure to vibration. However, there is almost no data about the status of animals’ organs in the post-contact period. Material and methods. The study was carried out in white male rats weighing 220-240 g, were exposed to 40 Hz vibration for 60 days 5 times a week for 4 hours a day. Histological and morphometric analysis was used to assess the sensorimotor cortex tissue and hepatorenal system. Results. A decrease in the total number of brain neurons, astraglial cells in rats in 30, 60 and 120 days of the post-exposure period was found. There was hyperemia in the portal and Central veins, an increase in the number of Kupffer cells in the liver tissue at the 30th, 60th, 120th day after the exposure. Decrease in the area of the Shumlyansky-Bowman capsule was recorded in the experimental rats’ kidney tissue 30 days after the end of vibration exposure; though there were no differences in the number of renal bodies in the tissue of white rats of the experimental and control groups at 60th and 120th day after the end of exposure to vibration. Conclusion. Morphological changes in the tissues of white rats exposed to prolonged vibration presented in the form of a decrease in the total number of neurons and astroglia cells in the brain tissue, a pronounced macrophage response in the liver tissue have been preserved in the post-contact period following the exposure to vibration.

TWO TYPES OF SERTOLI CELLS IN MAN

1969 ◽  
Vol 61 (1) ◽  
pp. 111-116 ◽  
Author(s):  
Svend G. Johnsen
Keyword(s):  
Nucleic Acids ◽  
Sertoli Cell ◽  
Sertoli Cells ◽  
Picric Acid ◽  
Type A ◽  
The Other ◽  
Type B ◽  
Iron Alum ◽  
Haematoxylin Staining

ABSTRACT By means of iron haematoxylin staining carefully differentiated by either picric acid or iron alum, Sertoli cells may be differentiated into two types, one (type A) containing unstained nuclei and the other (type B) intensely stained nuclei. No intermediate forms appear to be present. The two types are most easily found in the Sertoli-cell-only syndrome but they are present in the testes with spermatogenesis although type B is rare. The immature (pro-) Sertoli cells in infantile testes also display the two types. The two types of Sertoli cells differ in their degree of binding between nucleic acids and nucleic protein. The significance of the existence of these two types is discussed.

scholarly journals Spermatogenic cells of the prepuberal mouse: isolation and morphological characterization

1977 ◽  
Vol 74 (1) ◽  
pp. 68-85 ◽  
Author(s):  
AR Bellve ◽  
JC Cavicchia ◽  
CF Millette ◽  
DA O'Brien ◽  
YM Bhatnagar ◽  
...  
Keyword(s):  
Sertoli Cells ◽  
Morphological Characteristics ◽  
Type A ◽  
Cell Types ◽  
Specific Cell ◽  
Spermatogenic Cells ◽  
Type B ◽  
Primitive Type ◽  
Pachytene Spermatocytes ◽  
Type A Spermatogonia

A procedure is described which permits the isolation from the prepuberal mouse testis of highly purified populations of primitive type A spermatogonia, type A spermatogonia, type B spermatogonia, preleptotene primary spermatocytes, leptotene and zygotene primary spermatocytes, pachytene primary spermatocytes and Sertoli cells. The successful isolation of these prepuberal cell types was accomplished by: (a) defining distinctive morphological characteristics of the cells, (b) determining the temporal appearance of spermatogenic cells during prepuberal development, (c) isolating purified seminiferous cords, after dissociation of the testis with collagenase, (d) separating the trypsin-dispersed seminiferous cells by sedimentation velocity at unit gravity, and (e) assessing the identity and purity of the isolated cell types by microscopy. The seminiferous epithelium from day 6 animals contains only primitive type A spermatogonia and Sertoli cells. Type A and type B spermatogonia are present by day 8. At day 10, meiotic prophase is initiated, with the germ cells reaching the early and late pachytene stages by 14 and 18, respectively. Secondary spermatocytes and haploid spermatids appear throughout this developmental period. The purity and optimum day for the recovery of specific cell types are as follows: day 6, Sertoli cells (purity>99 percent) and primitive type A spermatogonia (90 percent); day 8, type A spermatogonia (91 percent) and type B spermatogonia (76 percent); day 18, preleptotene spermatocytes (93 percent), leptotene/zygotene spermatocytes (52 percent), and pachytene spermatocytes (89 percent), leptotene/zygotene spermatocytes (52 percent), and pachytene spermatocytes (89 percent).

Biological and Morphological Studies on Low-Leukemia-Strain Mice with Hodgkin's-Like Neoplasia Induced by Serial Cell-Free Transmission of Leukemic Organs of SJL/J Strain Mice

1968 ◽  
Vol 26 ◽  
pp. 210-211
Author(s):  
S. Fujinaga ◽  
K. Maruyama ◽  
C.W. Williams ◽  
K. Sekhri ◽  
L. Dmochowski
Keyword(s):  
Tissue Culture ◽  
Type A ◽  
Reticulum Cell ◽  
Type B ◽  
Viral Origin ◽  
Serial Transfer ◽  
Strain Mouse ◽  
Morphological Studies ◽  
Cell Neoplasm ◽  
Free Material

Yumoto and Dmochowski (Cancer Res.27, 2098 (1967)) reported the presence of mature and immature type C leukemia virus particles in leukemic organs and tissues such as lymph nodes, spleen, thymus, liver, and kidneys of SJL/J strain mice with Hodgki's-like disease or reticulum cell neoplasm (type B). In an attempt to ascertain the possibility that this neoplasia may be of viral origin, experiments with induction and transmission of this neoplasm were carried out using cell-free extracts of leukemic organs from an SJL/J strain mouse with spontaneous disease.It has been possible to induce the disease in low-leukemia BALB/c and C3HZB strain mice and serially transfer the neoplasia by cell-free extracts of leukemic organs of these mice. Histological examination revealed the neoplasia to be of either reticulum cell-type A or type B. Serial transfer is now in its fifth passage. In addition leukemic spleen from another SJL/J strain mouse with spontaneous reticulum cell neoplasm (type A) was set up in tissue culture and is now in its 141st serial passage in vitro. Preliminary results indicate that cell-free material of 39th tissue culture passage can reproduce neoplasia in BALB/c mice.

Anticoagulant Activity of β2-Glycoprotein I Is Potentiated by a Distinct Subgroup of Anticardiolipin Antibodies

1992 ◽  
Vol 68 (03) ◽  
pp. 297-300 ◽  
Author(s):  
Monica Galli ◽  
Paul Comfurius ◽  
Tiziano Barbui ◽  
Robert F A Zwaal ◽  
Edouard M Bevers
Keyword(s):  
Human Plasma ◽  
Anticoagulant Activity ◽  
Factor Xa ◽  
Type A ◽  
Lipid Vesicles ◽  
Dependent Manner ◽  
Type B ◽  
Distinct Subgroup ◽  
Glycoprotein I ◽  
Thrombin Formation

SummaryPlasmas of 16 patients positive for both IgG anticardiolipin (aCL) antibodies and lupus anticoagulant (LA) antibodies were subjected to adsorption with liposomes containing cardiolipin. In 5 of these plasmas both the anticardiolipin and the anticoagulant activities were co-sedimented with the liposomes in a dose-dependent manner, whereas in the remaining cases only the anticardiolipin activity could be removed by the liposomes, leaving the anticoagulant activity (LA) in the supernatant plasma. aCL antibodies purified from the first 5 plasmas were defined as aCL-type A, while the term aCL-type B was used for antibodies in the other 11 plasmas, from which 2 were selected for this study.Prolongation of the dRVVT was produced by affinity-purified aCL-type A antibodies in plasma of human as well as animal (bovine, rat and goat) origin. aCL-type B antibodies were found to be devoid of anticoagulant activity, while the corresponding supernatants containing LA IgG produced prolongation of the dRVVT only in human plasma.These anticoagulant activities of aCL-type A and of LA IgG's were subsequently evaluated in human plasma depleted of β2-glycoprotein I (β2-GPI), a protein which was previously shown to be essential in the binding of aCL antibodies to anionic phospholipids. Prolongation of the dRVVT by aCL-type A antibodies was abolished using β2-GPI deficient plasma, but could be restored upon addition of β2-GPI. In contrast, LA IgG caused prolongation of the dRVVT irrespective of the presence or absence of β2-GPI.Since β2-GPI binds to negatively-charged phospholipids and impedes the conversion of prothrombin by the factor Xa/Va enzyme complex (Nimpf et al., Biochim Biophys Acta 1986; 884: 142–9), comparison was made of the effect of aCL-type A and aCL-type B antibodies on the rate of thrombin formation in the presence and absence of β2-GPI. This was measured in a system containing highly purified coagulation factors Xa, Va and prothrombin and lipid vesicles composed of 20 mole% phosphatidylserine and 80 mole% phosphatidylcholine. No inhibition on the rate of thrombin formation was observed with both types of aCL antibodies when either β2-GPI or the lipid vesicles were omitted. Addition of β2-GPI to the prothrombinase assay in the presence of lipid vesicles causes a time-dependent inhibition which was not affected by the presence of aCL-type B or non-specific IgG. In contrast, the presence of aCL-type A antibodies dramatically increased the anticoagulant effect of β2-GPI. These data indicate that the anticoagulant activity of aCL-type A antibodies in plasma is mediated by β2-GPI.

PROTECTIVE EFFECT OF SOME SALTS 2-AMINOETHANESULFONIC ACID IN AN EXPERIMENTAL MODEL OF DEGENERATIVE SPINAL CORD INJURY

2020 ◽  
pp. 41-47
Author(s):  
Semeleva E.V. ◽  
Blinova E.V. ◽  
Zaborovsky A.V. ◽  
Vasilkina O.V. ◽  
Shukurov A.S.
Keyword(s):  
Spinal Cord ◽  
Morphological Changes ◽  
Male Rats ◽  
Zinc Salt ◽  
Control Group ◽  
Morphological Studies ◽  
Cord Injury ◽  
The Central Nervous System ◽  
Acid Compound ◽  
Lateral Sclerosis

In this work, we studied the pharmacological activity of zinc and magnesium salts of 2-aminoethanesulfonic acid in white non-linear male rats with amyotrophic lateral sclerosis, which was modeled by neurotoxicantsimplication into the pelvic part of spinal cord. After the reproduction of the pathology in animals, the indices of motor activity were recorded in the Rotarod test, and morphological studies of spinal cord sections stained according to Nisl in the Belshovsky modification were carried out. It was shown that the magnesium salt of 2-aminoethanesulfonic acid (compound LHT-317) to a greater extent reduces the development of motor disorders in experimental animals compared with the control group on the 4th day of observation. The course of intravenous administration of the studied compounds of 2-aminoethanesulfonic acid did not inhibit morphological changes in the spinal cord that develop in degenerative-dystrophic pathology of the central nervous system: connections. Moreover, if, against the background of treatment with zinc salt, the total area of motor zones in animals of the experimental group exceeded that of control rats, then the number of motoneurons did not differ from the control.

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