scholarly journals EVALUATION OF THE INFLUENCE OF ARBUSCULAR MYCORRHIZAL FUNGI ON ECONOMICALLY VALUABLE INDICATORS OF ONION

Ekosistemy ◽  
2020 ◽  
Vol - (21) ◽  
pp. 101-108
Author(s):  
S. F. Abdurashytov ◽  
V. I. Nemtinov ◽  
E. V. Puzanova ◽  
K. S. Gritsevich ◽  
I. V. Belova ◽  
...  
Keyword(s):  
Mycorrhizal Fungi ◽  
Field Experiments ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Field Studies ◽  
Dry Mass ◽  
Initial Assessment ◽  
Vegetable Crops ◽  
Bulb Formation ◽  
Southern Chernozem

Allium cepa L. is one of the main vegetable crops. It is actively used in food industry and medicine. The biochemical composition of plants, both bulbs and green leaves, in different periods of growth changes depending on the variety, environmental and agrotechnical conditions of plant cultivation. Representatives of the genus Allium are highly sensitive to the presence of arbuscular mycorrhiza (AM) in soil due to root development features. The aim of our work was to identify effective associations of AM fungi to increase the productivity and quality of onions in the conditions of southern chernozem. Pot and field experiments were carried out with associations of AM fungi and onion varieties from the collection of the Research Institute of Agriculture of Crimea. The initial assessment of the AM fungi collection was conducted in the pot with a sterile substrate under artificial lighting conditions according to the intensity of mycorrhizal colonization and the accumulation of phytomass of the storage plant. Field studies were carried out on southern carbonate chernozem in 2019. It is established that the AM fungi association M9 significantly exceeds the referent in the arbuscules abundance by 13.3 %. Association 1–16 showed the most considerable abundance of vesicles, which was 11.1–13.3 % higher than other variants of this experiment. The dry mass of onion shoots had a significant increase of 11.719.7 mg per plant (38.865.8 %) from inoculation by associations AM S1-4, 1-16 and M9. The positive effect of the AM fungi association 1-16 on the accumulation of phytomass in the onset phase of bulb formation and onion productivity of the onion variety Yaltinskiy plus and line 11A with an increase by 0.690.8 g / plant (31.351.7 %) and 1.11.3 t / ha (14.114.9 %), respectively, and an increase in the content of ascorbic acid by 18.524.4 % to the variant without treatment are shown.

Field inoculation with arbuscular mycorrhizal fungi in rehabilitation of mine sites with native vegetation, including Acacia spp.

2003 ◽  
Vol 16 (1) ◽  
pp. 131 ◽  
Author(s):  
J. Bell ◽  
S. Wells ◽  
D. A. Jasper ◽  
L. K. Abbott
Keyword(s):  
Mycorrhizal Fungi ◽  
Field Experiments ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Native Plant ◽  
Field Inoculation ◽  
Native Plant Species ◽  
Mycorrhizal Colonisation ◽  
Mine Sites ◽  
Fertiliser Application

Field experiments were conducted at rehabilitation sites at two contrasting mines in Western Australia. At both mines, Acacia spp. are important components of the rehabilitation ecosystem. At a mineral sands mine near Eneabba, dry-root inoculum of the arbuscular mycorrhizal (AM) fungus Glomus invermaium (WUM 10) was introduced into riplines with three rates of phosphate fertiliser application. Plants were assessed for mycorrhizal colonisation and phosphorus status. There was no plant growth benefit from inoculation. A considerable number of infective propagules of indigenous AM fungi was already present in the topsoil. The inoculant fungus as well as the indigenous AM fungi formed mycorrhizas, but only in a small number of Acacia and other native plant species. In a study of AM fungal inoculation at a gold mine rehabilitation site at Boddington, dry-root inoculum of G.�invermaium was applied to riplines prior to seeding. Despite apparently ideal environmental conditions, colonisation of native seedlings was limited. Possible reasons for this were investigated in further experiments that addressed environmental factors such as soil temperature and moisture and factors such as the age of the plant and presence of a colonised cover crop. Inoculum remained infective even under moist conditions in field soil for at least 4 months. Its infectivity decreased in parallel with falling temperatures. However, the level of infectivity present did not ensure extensive colonisation of native plants such as Acacia seedlings in the field. Susceptibility of Acacia seedlings to colonisation by AM fungi appeared to be seasonal, as colonisation increased with increasing daytime temperatures and daylight hours.

scholarly journals Use of the Signature Fatty Acid 16:1ω5 as a Tool to Determine the Distribution of Arbuscular Mycorrhizal Fungi in Soil

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Christopher Ngosong ◽  
Elke Gabriel ◽  
Liliane Ruess
Keyword(s):  
Fatty Acid ◽  
Mycorrhizal Fungi ◽  
Plant Root ◽  
Background Level ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Biomass Estimation ◽  
Lipid Fatty Acid ◽  
Neutral Lipid Fatty Acid ◽  
Root Symbionts

Biomass estimation of arbuscular mycorrhiza (AM) fungi, widespread plant root symbionts, commonly employs lipid biomarkers, predominantly the fatty acid 16:1ω5. We briefly reviewed the application of this signature fatty acid, followed by a case study comparing biochemical markers with microscopic techniques in an arable soil following a change to AM non-host plants after 27 years of continuous host crops, that is, two successive cropping seasons with wheat followed by amaranth. After switching to the non-host amaranth, spore biomass estimated by the neutral lipid fatty acid (NLFA) 16:1ω5 decreased to almost nil, whereas microscopic spore counts decreased by about 50% only. In contrast, AM hyphal biomass assessed by the phospholipid (PLFA) 16:1ω5 was greater under amaranth than wheat. The application of PLFA 16:1ω5 as biomarker was hampered by background level derived from bacteria, and further enhanced by its incorporation from degrading spores used as microbial resource. Meanwhile, biochemical and morphological assessments showed negative correlation for spores and none for hyphal biomass. In conclusion, the NLFA 16:1ω5 appears to be a feasible indicator for AM fungi of the Glomales group in the complex field soils, whereas the use of PLFA 16:1ω5 for hyphae is unsuitable and should be restricted to controlled laboratory studies.

scholarly journals Response of Arbuscular Mycorrhizal Fungi to Simulated Climate Changes by Reciprocal Translocation in Tibetan Plateau

2015 ◽  
Vol 43 (2) ◽  
pp. 488-493
Author(s):  
Zhaoyong SHI ◽  
Xubin YIN ◽  
Bede MICKAN ◽  
Fayuan WANG ◽  
Ying ZHANG ◽  
...  
Keyword(s):  
Tibetan Plateau ◽  
Arbuscular Mycorrhizal Fungi ◽  
Reciprocal Translocation ◽  
Mycorrhizal Fungi ◽  
Climate Changes ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Spore Density ◽  
The Tibetan Plateau ◽  
Colonization Frequency

Arbuscular mycorrhiza (AM) fungi are considered as an important factor in predicting plants and ecosystem responses to climate changes on a global scale. The Tibetan Plateau is the highest region on Earth with abundant natural resources and one of the most sensitive region to climate changes. To evaluate the complex response of arbuscular mycorrhizal fungi colonization and spore density to climate changes, a reciprocal translocation experiment was employed in Tibetan Plateau. The reciprocal translocation of quadrats to AM colonization and spore density were dynamic. Mycorrhizal colonization frequency presented contrary changed trend with elevations of quadrat translocation. Colonization frequency reduced or increased in majority quadrats translocated from low to high or from high to low elevation. Responses of colonization intensity to translocation of quadrats were more sensitive than colonization frequency. Arbuscular colonization showed inconsistent trend in increased or decreased quadrat. Vesicle colonization decreased with changed of quadrat from low to high elevations. However, no significant trend was observed. Although spore density was dynamic with signs of decreasing or increasing in translocated quadrats, the majority enhanced and declined respectively in descent and ascent quadrat treatments. It is crucial to understand the interactions between AM fungi and prairie grasses to accurately predict effects of climate change on these diverse and sensitive ecosystems. This study provided an opportunity for understanding the effect of climate changes on AM fungi.

scholarly journals FUNGOS MICORRÍZICOS ARBUSCULARES E DOSES DE FÓSFORO NO DESENVOLVIMENTO DE MUDAS DE GUANANDI

Nativa ◽  
2018 ◽  
Vol 6 (3) ◽  
pp. 246
Author(s):  
Edilson Nonato Da Silva ◽  
Aline Torquato Tavares ◽  
Cândida Pereira Da Silva ◽  
Tiago Alves Ferreira ◽  
João Victor Gonçalves Carline ◽  
...  
Keyword(s):  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Dry Mass ◽  
Mycorrhizal Colonization ◽  
University Campus ◽  
Experimental Station ◽  
Calophyllum Brasiliense ◽  
Shoot Mass ◽  
Different Levels ◽  
Forest Engineering

O experimento foi realizado na Estação Experimental de Engenharia Florestal do Campus Universitário de Gurupi, Universidade Federal do Tocantins (UFT), com objetivo de determinar a melhor dose de fósforo na presença de fungos arbusculares em mudas de guanandi (Calophyllum brasiliense Camb.) O delineamento experimental utilizado foi inteiramente casualizado com três repetições em esquema fatorial 6 x 2. Os tratamentos foram seis doses de fósforo (0; 400; 800; 1200; 1600 e 2000 g de P2O5 por m³) no substrato na presença e ausência de Fungos Micorrízos Arbusculares (FMAs). As características avaliadas foram altura das plantas, diâmetro do caule, massa seca da parte aérea (MSPA), massa seca da raiz (MSR), massa seca total (MST) e colonização micorrízica. Os diferentes níveis de adubação influenciaram a colonização micorrízica arbuscular. Nas doses 400 e 1600 g de fósforo por m3 de substrato observou-se maior porcentagem de colonização. A altura e o diâmetro das plantas foram significativamente afetados pela presença de P2O5. A massa seca da parte aérea e massa seca total não é afetada por doses de P2O5. O melhor desempenho das mudas de C. brasiliense ocorreu aos 90 dias com dose de 1.421 g de P2O5 por m3 de substrato.Palavras-chave: Calophyllum brasiliense Camb., adubação, micorrização. MYCORRHIZAL FUNGI AND PHOSPHORUS DOSES IN THE DEVELOPMENT OF GUANANDI SEEDLINGS ABSTRACT:The experiment was carried out at the Forest Engineering Experimental Station of the Federal University of Tocantins (UFT), University Campus of Gurupi, with the objective of determining the best dose of phosphorus in the presence of arbuscular fungi in guanandi (Calophyllum brasiliense Camb. The experimental design was completely randomized with three replicates in a 6 x 2 factorial scheme. The treatments were six doses of phosphorus (0, 400, 800, 1200, 1600 and 2000 g of P2O5 per m³) on the substrate in the presence and absence of Arbuscular mycorrhizal fungi (FMAs). The evaluated characteristics were plant height, stem diameter, shoot dry mass (MSPA), root dry mass (MSR), total dry mass (MST) and mycorrhizal colonization. The different levels of fertilization influenced arbuscular mycorrhizal colonization. At 400 and 1600 g of phosphorus per m3 of substrate, a higher percentage of colonization was observed. The height and diameter of the plants were significantly affected by the presence of P2O5. Dry shoot mass and total dry mass are not affected by P2O5 doses. The best performance of C. brasiliense seedlings occurred at 90 days with a dose of 1421 g of P2O5 per m3 of substrate.Keywords: Calophyllum brasiliense Camb., fertilization, mycorrhization. DOI:

scholarly journals Synergistic community responses of plants and arbuscular mycorrhizal fungi to extreme droughts in a cold-temperate grassland

2020 ◽  
Author(s):  
Wei Fu ◽  
Baodong Chen ◽  
Matthias Rillig ◽  
Wang Ma ◽  
Chong Xu ◽  
...  
Keyword(s):  
Fungal Community ◽  
Mycorrhizal Fungi ◽  
Community Dynamics ◽  
Environmental Changes ◽  
Environmental Filtering ◽  
Climate Extremes ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Temperate Grassland ◽  
Community Responses

Mutualistic associations between plants and arbuscular mycorrhizal (AM) fungi may have profound influences on their response to climate changes. Existing theories evaluate the effects of interdependency and environmental filtering on plant-AM fungal community dynamics separately; however, abrupt environmental changes such as climate extremes can provoke duo-impacts on the metacommunity simultaneously. Here, we experimentally tested the relevance of plant and AM fungal community responses to extreme drought (chronic or intense) in a cold temperate grassland. Irrespective of drought intensities, plant species richness and productivity responses were significantly and positively correlated with AM fungal richness and also served as best predictors of AM fungal community shifts. Notably, the robustness of this community synergism increased with drought intensity, likely reflecting increased community interdependence. Network analysis showed a key role of Glomerales in AM fungal interaction with plants, suggesting specific plant-AM fungal pairing. Thus, community interdependence may underpin climate change impact on plant-AM fungal diversity patterns in grasslands.

scholarly journals The Synergy of Arbuscular Mycorrhizal Fungi and Exogenous Abscisic Acid Benefits Robinia pseudoacacia L. Growth through Altering the Distribution of Zn and Endogenous Abscisic Acid

2021 ◽  
Vol 7 (8) ◽  
pp. 671
Author(s):  
Xiao Lou ◽  
Xiangyu Zhang ◽  
Yu Zhang ◽  
Ming Tang
Keyword(s):  
Abscisic Acid ◽  
Mycorrhizal Fungi ◽  
Black Locust ◽  
Robinia Pseudoacacia ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Am Symbiosis ◽  
Antioxidant Defense Systems ◽  
Robinia Pseudoacacia L ◽  
Zn Stress

The simultaneous effects of arbuscular mycorrhizal (AM) fungi and abscisic acid (ABA) on the tolerance of plants to heavy metal (HM) remain unclear. A pot experiment was carried out to clarify the effects of simultaneous applications of AM fungi and ABA on plant growth, Zn accumulation, endogenous ABA contents, proline metabolism, and the oxidative injury of black locust (Robinia pseudoacacia L.) exposed to excess Zn stress. The results suggested that exogenously applied ABA positively enhanced AM colonization, and that the growth of plants only with AM fungi was improved by ABA application. Under Zn stress, AM inoculation and ABA application increased the ABA content in the root/leaf (increased by 48–172% and 92%, respectively) and Zn content in the root/shoot (increased by 63–152% and 61%, respectively) in AM plants, but no similar trends were observed in NM plants. Additionally, exogenous ABA addition increased the proline contents of NM roots concomitantly with the activities of the related synthases, whereas it reduced the proline contents and the activity of Δ1-pyrroline-5-carboxylate synthetase in AM roots. Under Zn stress, AM inoculation and ABA application decreased H2O2 contents and the production rate of O2, to varying degrees. Furthermore, in the roots exposed to Zn stress, AM inoculation augmented the activities of SOD, CAT, POD and APX, and exogenously applied ABA increased the activities of SOD and POD. Overall, AM inoculation combined with ABA application might be beneficial to the survival of black locust under Zn stress by improving AM symbiosis, inhibiting the transport of Zn from the roots to the shoots, increasing the distribution of ABA in roots, and stimulating antioxidant defense systems.

scholarly journals Biocontrol of Sclerotium rolfsii in Groundnut by Using Microbial Inoculants

2017 ◽  
Vol 9 (1) ◽  
pp. 124-130 ◽  
Author(s):  
Khirood DOLEY ◽  
Mayura DUDHANE ◽  
Mahesh BORDE
Keyword(s):  
Mycorrhizal Fungi ◽  
Antioxidant Activities ◽  
Sclerotium Rolfsii ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Stem Rot ◽  
Mycorrhizal Dependency ◽  
Trichoderma Species ◽  
Arachis Hypogaea L ◽  
Microbial Strains

Sclerotium rolfsii (Sacc.) is the causal agent of stem-rot in groundnut (Arachis hypogaea L.)crop. With the increase in demand for the groundnut, control of stem-rot efficiently by microbial strains is fast becoming inevitable as the conventional system of chemicals is degrading our ecosystem. This investigation here emphasizes on inoculation of arbuscular mycorrhizal fungi (AMF) and Trichoderma species for growth achievement and disease control. The present investigation showed that these microbial strains were found to be worth applying as they stimulated growth and decreased harmful effects of S. rolfsii (cv. ‘Western-51’). The increased biochemical parameters and antioxidant activities also indicated their defence related activities in groundnut plants. In spite of positive attributes meted out by these microbial strains towards groundnut crop, the interaction among AM fungi and Trichoderma species seemed to be less co-operative between each other which were noted when mycorrhizal dependency and percent root colonization were observed. However, in summary more practical application of low-input AM fungi along with Trichoderma species may be needed for the advancement of modern agricultural systems.

Influence of arbuscular mycorrhizae on soil P dynamics, corn P-nutrition and growth in a ridge-tilled commercial field

2008 ◽  
Vol 88 (3) ◽  
pp. 283-294 ◽  
Author(s):  
Christine P Landry ◽  
Chantal Hamel ◽  
Anne Vanasse
Keyword(s):  
Arbuscular Mycorrhizae ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Dry Mass ◽  
Soil Disturbance ◽  
P Uptake ◽  
P Nutrition ◽  
Soil P ◽  
High Yields ◽  
Ridge Tillage

Ridge-tilled corn (Zea mays L.) could benefit from arbuscular mycorrhizal (AM) fungi. Under low soil disturbance, AM hyphal networks are preserved and can contribute to corn nutrition. A 2-yr study was conducted in the St. Lawrence Lowlands (Quebec, Canada) to test the effects of indigenous AM fungi on corn P nutrition, growth, and soil P in field cropped for 8 yr under ridge-tillage. Phosphorus treatments (0, 17, 35 kg P ha-1) were applied to AM-inhibited (AMI) (fungicide treated) and AM non-inhibited (AMNI) plots. Plant tissue and soil were sampled 22, 48 and 72 days after seeding (DAS). P dynamics was monitored in situ with anionic exchange membranes (PAEM) from seeding to the end of July. AMNI plants showed extensive AM colonization at all P rates. At 22 DAS, AMI plants had decreased growth in the absence of P inputs, while AMNI plants had higher dry mass (DM) and P uptake in unfertilized plots. The PAEM was lower in the AMNI unfertilized soils in 1998 and at all P rates in 1999, indicating an inverse relationship between P uptake and PAEM. At harvest, grain P content of AMNI plants was greater than that of AMI plants. In 1998, only AMI plants had decreased yield in the absence of P fertilization. In 1999, AMNI plants produced greater grain yield than AMI plants at all P rates. AM fungi improve the exploitation of soil P by corn thereby maintaining high yields while reducing crop reliance on P inputs in RT. Key words: Arbuscular mycorrhizae, ridge-tillage, soil P dynamics, corn, P nutrition

scholarly journals Haustorium Formation in Medicago truncatula Roots Infected by Phytophthora palmivora Does Not Involve the Common Endosymbiotic Program Shared by Arbuscular Mycorrhizal Fungi and Rhizobia

2015 ◽  
Vol 28 (12) ◽  
pp. 1271-1280 ◽  
Author(s):  
Rik Huisman ◽  
Klaas Bouwmeester ◽  
Marijke Brattinga ◽  
Francine Govers ◽  
Ton Bisseling ◽  
...  
Keyword(s):  
Medicago Truncatula ◽  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Phytophthora Palmivora ◽  
Living Plant ◽  
Mutualistic Symbiosis ◽  
Membrane Compartment ◽  
Microbe Interactions ◽  
Host Genes

In biotrophic plant-microbe interactions, microbes infect living plant cells, in which they are hosted in a novel membrane compartment, the host-microbe interface. To create a host-microbe interface, arbuscular mycorrhizal (AM) fungi and rhizobia make use of the same endosymbiotic program. It is a long-standing hypothesis that pathogens make use of plant proteins that are dedicated to mutualistic symbiosis to infect plants and form haustoria. In this report, we developed a Phytophthora palmivora pathosystem to study haustorium formation in Medicago truncatula roots. We show that P. palmivora does not require host genes that are essential for symbiotic infection and host-microbe interface formation to infect Medicago roots and form haustoria. Based on these findings, we conclude that P. palmivora does not hijack the ancient intracellular accommodation program used by symbiotic microbes to form a biotrophic host-microbe interface.

scholarly journals Stable C and N isotope natural abundances of intraradical hyphae of arbuscular mycorrhizal fungi

Mycorrhiza ◽  
2020 ◽  
Vol 30 (6) ◽  
pp. 773-780
Author(s):  
Saskia Klink ◽  
Philipp Giesemann ◽  
Timo Hubmann ◽  
Johanna Pausch
Keyword(s):  
Mycorrhizal Fungi ◽  
Isotope Composition ◽  
Fungal Spores ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Mineral Nutrient ◽  
Carbohydrate Source ◽  
Lipid Source ◽  
Am Fungus ◽  
C And N

Abstract Data for stable C and N isotope natural abundances of arbuscular mycorrhizal (AM) fungi are currently sparse, as fungal material is difficult to access for analysis. So far, isotope analyses have been limited to lipid compounds associated with fungal membranes or storage structures (biomarkers), fungal spores and soil hyphae. However, it remains unclear whether any of these components are an ideal substitute for intraradical AM hyphae as the functional nutrient trading organ. Thus, we isolated intraradical hyphae of the AM fungus Rhizophagus irregularis from roots of the grass Festuca ovina and the legume Medicago sativa via an enzymatic and a mechanical approach. In addition, extraradical hyphae were isolated from a sand-soil mix associated with each plant. All three approaches revealed comparable isotope signatures of R. irregularis hyphae. The hyphae were 13C- and 15N-enriched relative to leaves and roots irrespective of the plant partner, while they were enriched only in 15N compared with soil. The 13C enrichment of AM hyphae implies a plant carbohydrate source, whereby the enrichment was likely reduced by an additional plant lipid source. The 15N enrichment indicates the potential of AM fungi to gain nitrogen from an organic source. Our isotope signatures of the investigated AM fungus support recent findings for mycoheterotrophic plants which are suggested to mirror the associated AM fungi isotope composition. Stable isotope natural abundances of intraradical AM hyphae as the functional trading organ for bi-directional carbon-for-mineral nutrient exchanges complement data on spores and membrane biomarkers.

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