BIOTRANSFORMATION OF COMPONENTS OF PEA GRAIN IN FOOD AND FODDER PROTEIN CONCENTRATES

2020 ◽  
pp. 409-411
Author(s):  
R. Ulanova ◽  
V. Gulakova ◽  
V. Kolpakova
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Geotrichum Candidum ◽  
Food Protein ◽  
Protein Concentrate ◽  
Protein Concentrates

The data were obtained on the development of biotechnology of food protein concentrate from pea grains and bioconversion of cereal serum with Saccharomyces cerevisiae 121 yeast and a new strain of the fungus Geotrichum candidum 977 with the formation of a feed microbial-plant preparation.

Biological Processing of Pea Grain and Secondary Starch Raw Materials to Produce Food and Feed Protein Concentrates

2020 ◽  
Vol 36 (4) ◽  
pp. 49-58
Author(s):  
V.V. Kolpakova ◽  
R.V. Ulanova ◽  
L.V. Chumikina ◽  
V.V. Bessonov
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Amino Acid ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Mass Fraction ◽  
Geotrichum Candidum ◽  
Protein Concentrate ◽  
Protein Concentrates ◽  
Pea Flour ◽  
Nitrogenous Substances

The goal of the study was to develop a biotechnological process for the production of protein concentrates via bioconversion of pea flour and whey, a secondary product of starch manufacture. Standard and special methods were used to analyze the chemical and biochemical composition of protein concentrates (amino acid, carbohydrate, and fractional) of flour, whey and protein concentrates. It was established that pea flour contains 52.28-57.05% water-soluble nitrogenous substances, 23.04-25.50% salt-soluble, 2.94-4.69% alcohol-soluble compounds, 0-0.61% of soluble glutenine, 6.67-10.40% alkali-soluble glutenine and 5.96-10.86% insoluble sclerotic substances. A mathematical model and optimal parameters of the enzymatic extraction of pea protein with a yield of 65-70% were developed. Ultrasonic exposure increased the yield of nitrogenous substances by 23.16 ± 0.69%, compared with the control without ultrasound. The protein concentrate had a mass fraction of nitrogenous substances of 72.48 ± 0.41% (Nx6.25) and a complete amino acid composition. The microbial conversion by the Saccharomyces cerevisiae 121 and Geotrichum candidum 977 cultures of starch whey which remained after protein precipitation allowed us to obtain feed concentrates from biomass and culture liquid with a protein mass fraction of 61.68-70.48% (Nx6.25). Protein concentrates positively affected the vital signs of rats and their excretory products. A technological scheme was developed to test the complex pea grain and starch whey processing under pilot conditions. pea, protein concentrate, extracts, whey, bioconversion, Geotrichum candidum, Saccharomyces cerevisiae, chemical composition, amino acid composition

scholarly journals Type and Amount of Legume Protein Concentrate Influencing the Technological, Nutritional, and Sensorial Properties of Wheat Bread

2021 ◽  
Vol 11 (1) ◽  
pp. 436
Author(s):  
Nastasia Belc ◽  
Denisa Eglantina Duta ◽  
Alina Culetu ◽  
Gabriela Daniela Stamatie
Keyword(s):  
Wheat Bread ◽  
Soy Protein Concentrate ◽  
Protein Concentrate ◽  
Total Change ◽  
Protein Concentrates ◽  
Overall Acceptability ◽  
Protein Levels ◽  
Legume Protein ◽  
Bread Properties

Plant protein concentrates are used to enhance the nutritional quality of bread and to respond to the demand of consumers with respect to increased protein intake. In the present study, bread samples were produced using pea protein concentrate (PP) and soy protein concentrate (SP) substituting wheat flour by 5%, 10%, and 15%. The protein levels were between 1.2- and 1.7-fold (PP) and 1.1- and 1.3-fold (SP) higher than the control bread. The incorporation of 10% and 15% PP allowed for the achievement of a “high protein” claim. Water absorption was correlated with the protein contents of the breads (r = 0.9441). The decrease in bread volume was higher for the PP than SP incorporations, and it was highly negatively correlated with the protein content (r = −0.9356). Soy breads had a softer crumb than pea breads. The total change in crumb colour was higher in the PP than SP breads. The soy breads had an overall acceptability between 6.3 and 6.8, which did not differ (p > 0.05) from the control. PP breads were statistically less liked (p < 0.05). The results underlined that the choice of the type and amount of protein concentrates influenced the bread properties differently.

scholarly journals Grain of narrow-leaved lupine with inclusion of organic micronutrient complex OMC as an alternative to soybeans in protein concentrates

2021 ◽  
Vol 901 (1) ◽  
pp. 012022
Author(s):  
Z N Fedorova ◽  
Yu G Tkachenko ◽  
V G Bliadze
Keyword(s):  
Protein Content ◽  
Research Data ◽  
Fiber Content ◽  
High Protein ◽  
Protein Concentrate ◽  
Cattle Farm ◽  
Protein Concentrates ◽  
Kaliningrad Region ◽  
High Degree ◽  
Competitive Import

Abstract The article presents the research data on the use of high-protein extruded concentrates based on narrow-leaved lupine in the compound feed in combination with organic microelement complex OMEK-7 M (complex, microelement additive produced by CJSC “Bioamid”, Saratov) in order to replace soybean. The studies were carried out on a cattle farm in the settlement of Novgorodskoe, Guryevskii district, Kaliningrad region (Temp LLC). The object of the research were calves of black-and-wheat breed. It was found that due to the extrusion of lupine grain in combination with OMEC premix, a competitive, import-substituting soybean-based protein concentrate with a high degree of bioavailability of feed was obtained. It contains a sufficient protein content of 26% and a low fiber content of 4.05%, which is very important for calves in the dairy period.

Effect of Varying Concentrations of Phytate, Calcium, and Zinc on the Solubility of Protein, Calcium, Zinc, and Phytate in Soy Protein Concentrate

1993 ◽  
Vol 56 (1) ◽  
pp. 42-46 ◽  
Author(s):  
SANDRA R. GIFFORD-STEFFEN ◽  
FERGUS M. CLYDESDALE
Keyword(s):  
Soy Protein ◽  
Zinc Complex ◽  
Soy Protein Concentrate ◽  
Protein Concentrate ◽  
Protein Concentrates ◽  
Gastrointestinal Conditions ◽  
Soluble Calcium ◽  
Soluble Zinc

The phytate x Ca:Zn millimolar ratio was varied in a number of soy protein concentrates and subjected to a sequential pH treatment to simulate gastrointestinal conditions. In two independent studies, calcium was either decreased incrementally from 4.94 to 0.25 mmol or zinc was increased from 0.0036 to 0.55 mmol. At the lowest level of calcium, a significant increase (P &lt; 0.01) in soluble zinc and soluble phytate was Observed at pH 5.5, due to the possible formation of a soluble calcium-phytate-zinc complex. Soluble zinc significantly increased (P &lt; 0.01) only when the level of zinc was raised to 0.55 mmol. Neither soluble calcium nor soluble phytate was altered at this level.

scholarly journals Sour milk product with high protein content

2019 ◽  
Vol 21 (91) ◽  
pp. 79-83
Author(s):  
V. Rudiuk ◽  
V. Pasichnyi ◽  
T. Khorunzha ◽  
O. Krasulya
Keyword(s):  
Protein Content ◽  
Milk Protein ◽  
Control Sample ◽  
The Body ◽  
Milk Product ◽  
Protein Concentrate ◽  
Intellectual Activity ◽  
Protein Concentrates ◽  
Sour Milk ◽  
Short Time

The formulations of sour-milk products made by the thermostat method with the use of protein concentrates are developed. The classic technology of thermosetting is used as a basis, with preliminary preparation of mixtures based on normalized milk, milk protein concentrate (KMBS-65) and serum protein ultra filtration concentrate (KSB-UV-65), followed by pasteurization and suction. Concentrates used in dry form. Milk protein promotes nutrition of tissues not short-lived, but in the long-term, which is very important for intense physical activity, and high intellectual activity. Whey protein is rapidly absorbed and the nutrients that it carries with them, in short time, enter the tissues of the body, including muscle. This allows you to compensate for energy costs in a short time and improve the processes of exchange designed to normalize the work of organs and systems. Protein concentrates are used to improve the density of the bunch and reduce the degree of syneresis. The product is enriched with a concentrate and will act as an additional source of natural milk protein supplementation to the human body. The optimum component composition of the mixture – the sour milk product, using model milk samples and protein concentrates containing 3; 5; 7; 10% of total volume. Control sample, without protein concentrate. In the preparation of mixtures, the optimal temperature for the administration of protein concentrates was determined by a practical method. Experimental studies were carried out at the research laboratories of the National University of Food Technologies. The comparative characteristics of the physical and chemical characteristics (pH, titrated acidity, protein content) and organoleptic (appearance, color, consistency) of the indices in samples with different percentages of the introduced protein concentrates, with each other and with the control sample were performed. The changes of the indicators of the product during storage within 7 days.

scholarly journals Research of changes in individual physico-chemical parameters of yoghurts using whey protein concentrates

2019 ◽  
Vol 21 (91) ◽  
pp. 162-166
Author(s):  
N. B. Slyvka ◽  
O. Ya. Bilyk ◽  
O. R. Mikhailytska ◽  
Yu. R. Hachak
Keyword(s):  
Whey Protein ◽  
Whey Proteins ◽  
Lactobacillus Delbrueckii ◽  
Whey Protein Concentrate ◽  
Protein Concentrate ◽  
Chemical Parameters ◽  
Protein Concentrates ◽  
Organoleptic Characteristics ◽  
Physico Chemical ◽  
Rate Of Decline

The purpose of the work was to investigate the effect of whey proteins and dry whey concentrates on the change of titrated and active acidity during digestion. In order to stabilize the consistency in the production of low-fat yogurts, dry whey was selected that met the requirements of State Standard 4552:2006. It is used to improve the taste of finished products, to add flavor, to improve the texture, as well as to improve overall quality. In addition, dry whey protein concentrate WPC 80 Milkiland was used. The addition of whey protein concentrate does not detract from the organoleptic characteristics of a normalized mixture, which allows it to be used in yogurt technology. The addition of whey proteins has a significant effect on the duration of gel formation. Whey protein concentrate and dry whey reduce the duration of latent fermentation and flocculation stages. The data obtained allows us to predict that they accelerate the coagulation process. This effect is enhanced by increasing the dose of protein concentrates. Conducted coagulation of milk with a different dose and observed changes in titrated and active acidity during the fermentation. Yogurt culture YF-L903, which includes Streptococcus salivarius subsp., Thermophilus, Lactobacillus delbrűeckii subsp. Bulgaricus were used for fermentation. The highest growth rate of titrated acidity is recorded for option 1 (0.5% dry sucrose) and controls that for 4 hours. the fermentation reached 80 °T. The highest rate of decline in active acidity is recorded in option 1 (0.5% dry sucrose serum). All samples for 4 hours of fermentation reached 4.65–4.72 units. pH. Thus, the acidity slightly increases with increasing the dose of serum protein concentrate and does not increase with the use of dry whey.

scholarly journals Autolyzed Saccharomyces cerevisiae as a single cell protein for broiler diet

2019 ◽  
Vol 48 (1) ◽  
pp. 1-8
Author(s):  
M Moniruzzaman ◽  
MBR Mollah
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Body Weight ◽  
Weight Gain ◽  
Single Cell ◽  
Body Weight Gain ◽  
Daily Weight Gain ◽  
Cell Protein ◽  
Control Group ◽  
Protein Concentrate ◽  
Broiler Diet

Microbial protein often called as single cell proteins (SCP) are becoming a potential alternative to conventional protein rich ingredients in poultry diet. An experiment was conducted to know the possibility of using Saccharomyces cerevisiae derived SCP in broiler diet. A total of 96 male Ross-308 day old chicks were assigned to four diet comprising control (0% replacement), 25% replacement, 50% replacement and 100% replacement of protein concentrate with autolyzed S. cerevisiae. Each group had 6 replicates of 4 chicks in each. Body weight, body weight gain, daily weight gain, feed intake and feed conversion ratio were not affected (P>0.05) in case of 25% and 50% replacements. However, 100% replacement had resulted numerically negative effect as compared to control group. Dressing percentage and other carcass characteristics were not affected (P>0.05) in the replacement groups. The results of this study indicated that autolyzed S. cerevisiae might be used to replace protein concentrate at 25% to 50% level in broiler diet without affecting growth and carcass quality. Bang. J. Anim. Sci. 2019. 48 (1): 1-8

Determination of Carotene and Xanthophyll in Alfalfa Protein Concentrates

1971 ◽  
Vol 54 (4) ◽  
pp. 769-772
Author(s):  
B E Knuckles ◽  
S C Witt ◽  
R E Miller ◽  
E M Bickoff
Keyword(s):  
Protein Concentrate ◽  
Protein Concentrates ◽  
Aoac Method

Abstract Methods for the determination of carotene and xanthophyll in dried protein concentrates prepared during the wet fractionation of alfalfa have been studied. The AOAC method for carotene and xanthophyll in dried forages, 39.018–39.023 (11th Ed.), proved most satisfactory for analysis of the dried whole juice. The modification of a method developed earlier in this laboratory for dried forages proved most effective for analysis of a protein concentrate, high in xanthophyll and carotene, prepared from the whole juice. The modified procedure gave significantly higher values for carotene and xanthophyll in this protein-xanthophyll concentrate than the AOAC method.

Ocena oddziaływania na grzyby drożdżopodobne olejku szałwiowego (Oleum Salviae)

2019 ◽  
Vol 20 (3) ◽  
Author(s):  
Anna Kędzia ◽  
Elżbieta Hołderna-Kędzia
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Alimentary Tract ◽  
Folk Medicine ◽  
Growth Control ◽  
Antimicrobial Properties ◽  
Chemical Compounds ◽  
Geotrichum Candidum ◽  
Rhodotorula Rubra ◽  
Oral Candidosis ◽  
Ancient Times

Introduction. The essential oils were known and used in ancient Times. The oil and extracts of sage are utilized in folk medicine and for food condiment, in cosmetics and perfumes. Sage (Salvia officinalis) belonging to the Labiatae family. Plant grow all over the world. Sage has a number of properties in it antiflogistic and antimicrobial. The oil is used for the treatment of different kind of diseases, including bronchitis, cough, arthritis, rheumatism, ulcers, inflammation of skin, alimentary tract and in Alzheimer’s disease. Etheric oil contain following compounds: α- and β-thujone, 1,8-cineole, camphor, borneol, α-pinene, β-pinene, β-caryophyllene, β-sabinene, limonene, α-humulene, myrcene, α-terpineol, viridiflorol and camphene. The chemical compounds of the oil have antioxidant and antimicrobial properties. Aim. The aim of the dates was to evaluate the susceptibility of yeastlike fungi to sage. Material and methods. A total 30 strains of yeastlike fungi isolated from patients with oral candidosis was tested. The strains were identified with system API 20 C AUX (BioMèrieux), production chlamydospore and pseudohyphe. The susceptibility (MIC) yeastlike fungi to sage oil was determined by means plate dilution technique in Sabouraud’s agar. The suspension contained 105 CFU per spot were spread Steers replicator over the surface of agar containing oil or without sage agar plates (strains growth control). Inoculated agar plates were incubated in aerobic conditions at 37°C for 24-48 hrs. The MIC was defined as a lowest concentration of the oil inhibited growth of fungal strains. Results. The results indicated that the sage oil was active against yeastlike fungi in concentrations 0.5-≥ 2.0 mg/ml. The MIC for 66% strains from genus Candida albicans ≥ 2.0 mg/ml. Similarly C. krusei strains were susceptible in range 0.5-≥ 2.0 mg/ml. The oil was less active towards C. glabrata and C. tropicalis strains (MIC 1.0-≥ 2.0 mg/ml). The most susceptible were the strains from genus of C. parapsilosis. The growth was inhibited within the range from 0.5 to 1.0 mg/ml. The strain from genus of Rhodotorula rubra was susceptible on 0.12-0.5 mg/ml and Saccharomyces cerevisiae on 0.25 mg/ml. Conclusions. Sage oil showed antifungal activity. The more susceptible to oil were the strains of Candida parapsilosis, Rhodotorula rubra and Saccharomyces cerevisiae. Oil was less active toward strains Candida glabrata, Candida tropicalis and Geotrichum candidum.

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