scholarly journals COMPREHENSIVE EVALUATION OF EXPRESSION PLATFORM: CHERRY PICKING THE ‘RIGHT’ TO ACCOMPLISH THE ‘BEST

2020 ◽  
pp. 143-165
Author(s):  
Shailendra Gaur ◽  
Kinjal Bhadresha ◽  
Baldev Patel ◽  
Meenu Saraf ◽  
Rakesh Rawal
Keyword(s):  
Cho Cells ◽  
Comprehensive Evaluation ◽  
Regulatory Elements ◽  
The Other ◽  
Transcription System ◽  
Mammalian Expression ◽  
Expression Platform ◽  
Active Transcription ◽  
The Right ◽  
Almost All

Mounting demands for biosimilars and continuous emphasis on cost reduction of drugs have motivated researchers across the globe to widen the horizon of screening in CHO cells based bio-production. Various groups have exploited multiple phenomena in CHO cells for better expression and improved quality of therapeutic protein, for example use of transposons, regulatory elements, engineering of cell lines for site specific integrations or knock-in/knockout models to achieve the desired results. The current study presents the data for evaluation of three mammalian expression platforms available in market having three different approaches for improving productivity. One, the MTX based platforms for gene amplification, GS knock-down based selectionsystem and UCOE based active transcription system. To evaluate the platforms stringently, stable pools of both mAb and non-mAb molecules were accessed. Our results suggested that UCOE based platform have performed exceedingly well in monoclonal antibody (mAb) expression in comparison to the other two platforms. Whereas, for non mAb molecules, UCOE based platform was comparable to other two platforms. On evaluating all the molecules, CHOGS (-) system was found least productive as compared to the other two platforms in almost all cases.

Predict

2013 ◽  
Author(s):  
Gautam Shroff
Keyword(s):  
Video Game ◽  
Eye Gaze ◽  
Laws Of Nature ◽  
The Other ◽  
The Future ◽  
Scientific Mind ◽  
Multiple Regions ◽  
The Right ◽  
Almost All ◽  
Hand Image

‘Predicting the future’—the stuff of dreams one might imagine; the province of astrologers and soothsayers, surely. Perhaps not, the scientific mind might retort: after all, is it not the job of science to discover laws of nature, and thereby make precise, verifiable predictions about the future? But what if we were to claim that prediction is neither fanciful nor difficult, and not even rare. Rather, it is commonplace; something that we all accomplish each and every moment of our lives. Some readers may recall the popular video game, pong, where the goal is to ‘keep the puck in play’ using an electronic paddle. Figure 2 shows images of two different pong games in progress. In addition to the paddle and puck, the players’ eye gaze is also being tracked. The image on the left shows the player’s eyes tracking the puck itself. On the other hand, in the right-hand image, the player is already looking at a point where she expects the puck to travel to. The player on the left is reactive; she simply tracks the puck, and as the game gets faster, she eventually misses. The right player, in contrast, is able to predict where the puck will be, and most of the time she gets it right. Further, we often see her eyes dart faster than the puck to multiple regions of the field as she appears to recalculate her prediction continuously. What kind of player do you think you are? As it happens, almost all of us are predictive players. Even if we have never played pong before, we rapidly begin predicting the puck’s trajectory after even a few minutes of playing. The ‘reactive player’ in this experiment was in fact autistic, which apparently affected the person’s ability to make predictions about the puck’s trajectory. (The neurological causes of autism are still not well known or agreed upon; the recent research from which the images in Figure 2 are taken represent new results that might shed some more lightonthisdebilitatingcondition.) So it appears that prediction, as exhibited by most pong players, is far from being a rare and unusual ability. It is in fact a part and parcel of our everyday lives, and is present, to varying degrees, in all conscious life.

Exceptions to Mutual Trust: Children's Use of Second-Order Beliefs in Responsibility Attribution

1987 ◽  
Vol 10 (2) ◽  
pp. 207-223 ◽  
Author(s):  
Nicola Yuill ◽  
Josef Perner
Keyword(s):  
Second Order ◽  
The Other ◽  
Cooperative Interaction ◽  
Road User ◽  
Mutual Trust ◽  
Responsibility Attribution ◽  
Right Of Way ◽  
The Difference ◽  
The Right ◽  
Almost All

Children of 6 to 9 years and adults judged a story protagonist's degree of blame for a traffic accident. All stories depicted a collision between a protagonist, who had the right of way, and another road user. Stories differed, however, in protagonist's second-order belief about the other road-user's knowledge. For instance, in one story, the protagonist mistakenly thought that the other had noticed her coming and that she could therefore rely on him abiding by the priority rule (principle of mutual trust) and grant her the right of way. This story contrasted with one where the protagonist knew that the other had not seen her and so was not justified in claiming priority. Most 7 and 8-year-old children understood the difference in second-order belief and about half of them were also able to make the correct responsibility attribution that the mistaken protagonist, thinking the other character knew, was less to blame for the accident than the one who knew about the other's ignorance. By 9 years, almost all children understood second-order beliefs and three-quarters were also able to make the correct responsibility attribution. The application of second-order beliefs to the principle of mutual trust is discussed in relation to communication failures and cooperative interaction.

scholarly journals International Methodological Basics of Electoral Law (From Antiquity to Modern Times: Philosophy-Legal Dimension)

wisdom ◽  
2021 ◽  
Vol 18 (2) ◽  
pp. 103-113
Author(s):  
Armen HARUTYUNYAN
Keyword(s):  
The Other ◽  
The Political ◽  
Political Rights ◽  
The Social ◽  
State Governance ◽  
The Republic ◽  
The One ◽  
The Right ◽  
Almost All ◽  
Electoral Law

The contemporary democratic states consider the concept of political rights, especially the right to vote as a fundamental pillar above all other rights. The political rights are dominant only due to their implementation: people have an opportunity to exercise their power on the one hand, and transfer their power without any political upheavals on the other. In this regard, it is worth highlighting that the political rights are one of the corner-stone rights for modern democratic rule of law. According to this thesis, we can persist that the problems of the realization of political rights are decisive and highly important even for the declared and transitional democratic states. In this respect, the Republic of Armenia is no exception as the problems of the implementation of political rights are definitely the electoral rights. These rights are among the most acute social problems that young Armenian democracy has faced after the independence. The issues in implementation of the political rights are steadily coupled with the problems of imposing punishments for crimes directed against political rights. As the experience of the Republic of Armenia has shown, the number of crimes directed against political rights has increased over the years. The tendency of the growth of the above-mentioned crimes has objective and subjective reasons. Among the objective reasons, we can note the transitional character of Armenian democracy. As for justice, it should be noted that such problems are inherent in almost all transitional states and especially, for modern countries. It is easier to understand, when we observe the experience of communities, which try to pass from the totalitarian rails of state governance to democratic ones. From the other side, the social and economic reasons of the state, poverty of the population can be considered as an objective reason. In terms of subjective reasons, firstly, the disproportionate punishment for crimes directed against political rights should be pointed out, which are the central obstacles for the implementation of political rights.

scholarly journals Rereading Comprehension Pedagogies: Toward a Dialogic Teaching Ethic that Honors Student Sensemaking

2013 ◽  
Vol 1 ◽  
Author(s):  
Maren Aukerman
Keyword(s):  
Reading Comprehension ◽  
Reading Instruction ◽  
The Other ◽  
Teaching Ethic ◽  
Dialogic Teaching ◽  
Textual Interpretation ◽  
The Right ◽  
Almost All ◽  
Textual Meaning ◽  
Take All

This conceptual essay critiques reading comprehension pedagogies that are part of the current educational landscape.  I argue that comprehension pedagogy generally reflects one of three differing orientations, each with its own assumptions about what comprehension is:  comprehension-as-outcome pedagogies, which emphasize getting textual meaning “right”; comprehension-as-procedure pedagogies, which emphasize knowing the “right” ways doing reading; and comprehension-as-sensemaking pedagogies, which take all textual interpretation seriously, regardless of “rightness.”  Comprehension-as-sensemaking pedagogies, in turn, can be distinguished as either primarily responsive – aimed at surfacing student understandings – or primarily dialogic – aimed at getting student understandings to refract.  Arguably, comprehension-as-outcome pedagogy dominates current reading instruction.  A focus on measuring and teaching toward “right” interpretations permeates almost all aspects of comprehension pedagogy even when one of the other orientations toward comprehension pedagogy is also at play.  While seemingly intuitive, this overarching outcome emphasis reifies textual meaning in ways that are both theoretically and ethically problematic.  I make the case that comprehension-as-sensemaking pedagogy should become primary instead.  I propose that comprehension-as-outcome and comprehension-as-procedure pedagogies should not be abandoned, but should be subordinated to dialogic comprehension-as-sensemaking pedagogy so that students’ textual sensemaking is more fully heard, respected, and examined in reading classrooms.

Assessment of knowledge of oral health hygiene among coastal guards in the port trust of south Tamil Nadu - A public health awareness survey

2020 ◽  
Vol 11 (SPL3) ◽  
pp. 1861-1868
Author(s):  
Bianca Princeton ◽  
Abilasha R ◽  
Preetha S
Keyword(s):  
Lung Cancer ◽  
Oral Health ◽  
Oral Hygiene ◽  
Tamil Nadu ◽  
Asbestos Exposure ◽  
The Other ◽  
Oral Manifestations ◽  
Illegal Activities ◽  
The Right ◽  
Oral Hygiene Habits

Oral hygiene is defined as the practice of keeping the mouth clean and healthy, by brushing and flossing to prevent the occurrence of any gum diseases like periodontitis or gingivitis. The main aim of oral health hygiene is to prevent the buildup of plaque, which is defined as a sticky film of bacteria and food formed on the teeth. The coastal guard is an official who is employed to watch the sea near a coast for ships that are in danger or involved with illegal activities. Coastal guards have high possibilities of being affected by mesothelioma or lung cancer due to asbestos exposure. So, a questionnaire consisting of 20 questions was created and circulated among a hundred participants who were coastal guards, through Google forms. The responses were recorded and tabulated in the form of bar graphs. Out of a hundred participants, 52.4% were not aware of the fact that coastal guards have high chances of developing lung cancer and Mesothelioma. 53.7% were aware of the other oral manifestations of lung cancer other than bleeding gums. Majority of the coastal guards feel that they are given enough information about dental hygiene protocols. Hence, to conclude, oral hygiene habits have to be elaborated using various tools in the right manner to ensure better health of teeth and gums.

JUAL BELI TANAH YANG DILAKUKAN TANPA AKTA PPAT

2018 ◽  
Vol 4 (1) ◽  
pp. 89-107
Author(s):  
Cheri Bayuni Budjang
Keyword(s):  
Everyday Life ◽  
Government Regulation ◽  
Land Rights ◽  
The Other ◽  
Legal Certainty ◽  
Land Registration ◽  
The Future ◽  
The Right ◽  
Laws And Regulations

Buying and selling is a way to transfer land rights according to the provisions in Article 37 paragraph (1) of Government Regulation Number 24 of 1997 concerning Land Registration which must include the deed of the Land Deed Making Official to register the right of land rights (behind the name) to the Land Office to create legal certainty and minimize the risks that occur in the future. However, in everyday life there is still a lot of buying and selling land that is not based on the laws and regulations that apply, namely only by using receipts and trust in each other. This is certainly very detrimental to both parties in the transfer of rights (behind the name), especially if the other party is not known to exist like the Case in Decision Number 42 / Pdt.G / 2010 / PN.Mtp

Visual asymmetries during face encoding Increased dwell time and fixations in the upper and left hemifields

2018 ◽  
Author(s):  
Fatima Maria Felisberti
Keyword(s):  
Face Recognition ◽  
Eye Movements ◽  
Visual Field ◽  
Face Processing ◽  
Dwell Time ◽  
The Other ◽  
Environmental Cues ◽  
Reading Habits ◽  
The Right

Visual field asymmetries (VFA) in the encoding of groups rather than individual faces has been rarely investigated. Here, eye movements (dwell time (DT) and fixations (Fix)) were recorded during the encoding of three groups of four faces tagged with cheating, cooperative, or neutral behaviours. Faces in each of the three groups were placed in the upper left (UL), upper right (UR), lower left (LL), or lower right (LR) quadrants. Face recognition was equally high in the three groups. In contrast, the proportion of DT and Fix were higher for faces in the left than the right hemifield and in the upper rather than the lower hemifield. The overall time spent looking at the UL was higher than in the other quadrants. The findings are relevant to the understanding of VFA in face processing, especially groups of faces, and might be linked to environmental cues and/or reading habits.

Challenges of Media Education Standards in Georgia

2017 ◽  
Vol 2 (2) ◽  
Author(s):  
Marine Vekua
Keyword(s):  
Working Conditions ◽  
Teaching Methods ◽  
International Standards ◽  
The Other ◽  
Historical Background ◽  
Journalism Education ◽  
Education Standards ◽  
Modern Media ◽  
The One ◽  
The Right

The main goal of this research is to determine whether the journalism education of the leading media schools inGeorgia is adequate to modern media market’s demands and challenges. The right answer to this main questionwas found after analyzing Georgian media market’s demands, on the one hand, and, on the other hand, differentaspects of journalism education in Georgia: the historical background, development trends, evaluation ofeducational programs and curricula designs, reflection of international standards in teaching methods, studyingand working conditions.

Starość w nauczaniu św. Bazylego Wielkiego

Vox Patrum ◽  
2011 ◽  
Vol 56 ◽  
pp. 339-348
Author(s):  
Bogdan Czyżewski
Keyword(s):  
Old Age ◽  
Spiritual Maturity ◽  
The Other ◽  
Interesting Aspect ◽  
Other Hand ◽  
Physical Suffering ◽  
Way Of Thinking ◽  
Almost All

Although St. Basil did not live 50 years, the topic of the old age appears in his works quite often. On the other hand, it is clear that Basil does not discuss this issue in one par­ticular work or in the longer argumentation. The fragmentary statements about old age can be found in almost all his works, but most of them can be found in the correspondence of Basil. In this paper we present the most important ad the most interesting aspect of teach­ing of Basil the Great. As these certificates show that the bishop of Caesarea looked at the old age maturely, rationally estimated passage of time, which very often makes a man different. He experienced it, for example as a spiritual and physical suffering, which often were connected with his person. He saw a lot of aspect of the old age, especially its advan­tages – spiritual maturity and wisdom. What is more, he pointed also to passage of time, which leads a man to eternity, which should be prepared to, regardless how old he is. In his opinion fear is not seen opinions of St. Basil present really Christian way of thinking, well-balanced and calm.

Sign in / Sign up

Export Citation Format

Share Document