scholarly journals Biochemical and Safety Examination of Ethanol Extract of Justicia Carnae on PHZ -Produced Anaemia in Wistar Rats

2020 ◽  
Vol 13 (1) ◽  
pp. 74-82
Author(s):  
Igwe, K ◽  
Ikpeazu V ◽  
Otuokere I
Keyword(s):  
Body Weight ◽  
Enzyme Activities ◽  
Leukocyte Count ◽  
Hematological Parameters ◽  
Negative Control ◽  
Total Leukocyte Count ◽  
Control Group ◽  
Serum Enzyme ◽  
Differential Leukocyte Count ◽  
Treatment Groups

The leaves of Justicia carnae were screened for a hematological and biochemical response using serum enzyme activities in rats. Twenty-five rats were used in this research and were grouped into 5 of 5 rats each. Group 1 was a negative control group and was treated with distilled water. Groups 2 entreated anaemic, 3, 4 and 5 were treatment groups and received 100, 200 and 400 mg/kg body weight of the J. carnae extract, respectively. Anaemic was induced with Phenylhydrazine (PHZ). The rats were treated for 14 days, thereafter was sacrificed and blood collected from the heart for analysis. The effect of J. carnae extract was checked on hematological parameters and serum enzyme activities. All results in treatment groups were compared with the untreated anaemic group 2 at statistical confidence p<0.05. The normal control group saved as a reference point. There was a progressive elevation of haematological parameters as the dose of the extract increased from 100, 200, to 400 mg/kg body weight compared to the untreated anaemic group. Hematological parameters, PCV, RBC, Haemoglobin increased significantly at (p<0.05). Total leukocyte count elevated but not significant. Differential leukocyte count indicated very mild lymphopenia neutrophilia, monocytopenia, and eosinopenia but were not significant. Clinical biochemical parameters, AST, ALT and ALP showed slight decrease but not significant. Creatinine, total protein, urea, and bilirubin mildly increased as the dose of the extract increased but within normal range and so was not statistically significant. The MCV, MCH and MCHC values suggested normocytic normochromic anaemic condition. It was concluded that the extract of J. carnae is safe to blood cells, liver, and kidney marker enzymes at doses < 400 mg/kg body weight.

scholarly journals Screening for Toxic and Haematological Effect of Ethanol Extract of Azadirachta Indica in Wistar Rats Using Some Serum Enzymes as Markers

2020 ◽  
Vol 13 (1) ◽  
pp. 113-119
Author(s):  
K.K. Igwe ◽  
I.E. Otuokere ◽  
O. V. Ikpeazu
Keyword(s):  
Body Weight ◽  
Azadirachta Indica ◽  
Leukocyte Count ◽  
Negative Control ◽  
Serum Enzymes ◽  
Haematological Parameters ◽  
Total Leukocyte Count ◽  
Control Group ◽  
Differential Leukocyte Count ◽  
Treatment Groups

The leaves of Azadirachta indica plant was screened for haematological, toxic and serum enzyme activities in rats. Twenty rats were used and were grouped into 4 of 5 rats each. Group 1 was the negative control group administered distilled water. Groups 2, 3, and 4 were the treatment groups received 200, 400 and 800 mg/kg body weight of the A. indica extract respectively. The rats were dosed for 14 days, thereafter were sacrificed and blood collected by cardiac puncture for analysis. The effect of A. indica extract was checked on haematological parameters and serum enzymes activities. All results in treatment groups were compared with the normal control at statistical confidence of 95% (p<0.05). There was progressive reduction of haematological parameters as the dose of the extract increased from 200, 400, to 800 mg/kg body weight. Haematological parameters, PCV, RBC, Haemoglobin showed decrease value which was not statistically significant at (p<0.05). Total leukocyte count, showed progressively elevation by the extract though not statistically significant. Differential leukocyte count indicated very mild lymphocytosis neutropeania, monocytopeania and eosinopeania which were not statistically significant. Clinical biochemical parameters, A. indica extract demonstrated normal levels of the serum enzymes (AST, ALT and ALP) though there was slight decrease in a dose dependent fashion.Total protein was within normal range. The normal MCV, MCH and MCHC values suggests normocytic normochromic anaemic condition. The extract of A. indica is safe to blood cells, liver and kidney marker enzymes at dose < 800 mg/kg body weight.

scholarly journals Evaluation of Anthelmintic Efficacy of Ivermectin, Levamisole HCl and Albendazole Administered through Different Routes against Naturally Occuring Gastrointestinal Nematodiasis in Black Bengal Goat Inducing Hematological Parameters and Live Weight Indices

2016 ◽  
Vol 5 (1) ◽  
pp. 26-34 ◽  
Author(s):  
M Aktaruzzaman ◽  
MS Islam ◽  
MMI Hasan ◽  
MJU Bhuiyan ◽  
MM Hossain ◽  
...  
Keyword(s):  
Body Weight ◽  
Hematological Parameters ◽  
Live Weight ◽  
Gastrointestinal Nematodes ◽  
Total Leukocyte Count ◽  
Control Group ◽  
Counting Method ◽  
Blood Samples ◽  
Group A ◽  
Group D

This study aimed to evaluate the efficacy of ivermectin, levamisole HCl and albendazole against gastrointestinal nematodes in naturally infected goats of government goat development farm, Sylhet, Bangladesh. The study included 52 black Bengal breed of which 30 were naturally infected and randomly selected 20 on the basis of their weight and egg count. Twenty black Bengal goats of 12-13 month old irrespective of sex infested with gastrointestinal nematodes were selected for this experiment and randomly divided into four equal groups (group A, B, C and D) where each group consisted of 5 goats and goats of group D were kept as control group. One injectable ivermectin (200?gkg-1 body weight, S/C) preparations (Techno Drugs Ltd. Bangladesh) and two solid levamisole HCl, albendazole (7.5 mgkg-1 body weight, orally) preparations (The ACME Laboratories Ltd. and Square Pharmaceuticals Ltd. Bangladesh) were used for positive control of gastrointestinal nematodes as group A, B and C. Goats of group D was kept as control without giving any treatment. Before trials (day 0), total egg count, blood samples and initial body weight were recorded. During the study period the fecal and blood samples were collected directly from rectum and examined on 7th, 14th, 21st and 28th day using McMaster fecal egg counting method. Body weight was recorded on day 28 following the treatments. The results of the comparative efficacies of different anthelmintic of ivermectin was 86.75%, followed by levamisole HCl 85.07% and albendazole 92.81%. McMaster fecal egg counting method discloses the percentage of Haemonchus spp. (15.38%), with Trichostrongylus spp, Strongyloides spp., and Cooperia spp. also present. After treatment with ivermectin, levamisole HCl and albendazole, Total Erythrocyte Count (TEC), Hemoglobin (Hb) content and Packed Cell Volume (PCV) were increased significantly (p<0.01 and p>0.05) in goats but Erythrocyte Sedimentation Rate (ESR) and Total Leukocyte Count (TLC) were decreased significantly (p<0.05 and p>0.01) in all treated goat and body weight was increased significantly (p<0.01) on day 28.International Journal of Natural Sciences (2015), 5(1) 26-34

scholarly journals Immunomodulator Effect of Lemongrass Extract (Cymbopogon nardus L.) to Increase Immune Cells as a Precaution Against SARS-CoV-2

2021 ◽  
Vol 4 (2) ◽  
pp. 73
Author(s):  
Putri Ayu Ika Setiyowati ◽  
Rofiatun Solekha ◽  
Sri Bintang Sahara Mahaputra Kusuma Negara ◽  
Reny Rosalina
Keyword(s):  
Body Weight ◽  
Optimal Dose ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Antioxidant Compounds ◽  
Optimal Dosage ◽  
Skin Injury ◽  
Treatment Groups

Introduction: In humans, Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV-2) can damage some tissue when the immune systems was decrease. Natural product from the plant often used to improve immune response against microorganism including virus. This study aimed to determine the potential antioxidant of lemongrass extract (C . nardus) with various dosage that can provide immunomodulatory effects and find an optimal dosage to be used.  Methods: The method used observasional analytic, using animal model of 30 male mice strain BALB/C, weight 25-30 gram, divided into 5 groups; the positive control group was given 0.05 mL of  0.05% CMC within 14 days, negative control group was given IMBOOST® tablet 200 mg/kg body weight (bw) within 14 days, treatment groups  were given C. nardus extract with various doses 50 mg/kg bw, 150 mg/kg bw, and 300 mg/kg bw.  In day 21 all group were injected with 0,2 ml of  pathogen bacterial (S. aureus). Blood samples were taken three times: 7th day, 14th day, and 21th day. Results: The results showed that lemongrass extract (C. nardus) was able to influence the leukocyte and lymphocytes count with significant different (p<0.05). The optimal dose is 150 mg/kg body weight. Conclusion: The antioxidant compounds that contain in the C. nardus extract have an ability to increasing the immune system in the dose 150 mg/kg bw , but in the dose 300 mg/kg bw became toxic that can make a skin injury or death in animal test.

Exploring the Effect of Terminalia Catappa Fruit Extract in Rheumatoid Arthritis: an Evaluation of Behavioural, Hematologiocal and Histopathological Parameters

2020 ◽  
Vol 20 ◽  
Author(s):  
Tapan Behl ◽  
Rashita Makkar ◽  
Sandeep Arora
Keyword(s):  
Rheumatoid Arthritis ◽  
Body Weight ◽  
Disease Control ◽  
Wistar Rats ◽  
Hematological Parameters ◽  
Control Group ◽  
Fruit Extract ◽  
Joint Tissue ◽  
Treatment Groups ◽  
Terminalia Catappa

Background and Objective: Rheumatoid arthritis (RA) is an autoimmune disease characterized by failure of spontaneous resolution of inflammation with lifetime perseverance, becoming principal cause of inactivity. Many conventional therapies are in use but the side effects associated with them are equally problematic. Therefore an urge to explore advanced therapeutic approaches with least adverse events has seeded the idea of discovering newer herbal drugs and their respective constituents in management of RA. The current research explores anti-arthritic activity of Terminalia catappa fruits hydroalcoholic extract in CFA induced Wistar rats. Methods: The fruits were obtained, washed, dried and processed to obtain hydroalcoholic powdered extract which was dissolved in water and given orally to the rats. Wistar rats of either sex were used in the study. The animals were injected with 0.1 ml of complete freund’s adjuvant (CFA) in footpad of left hind paw. The treatment was initiated from day 0 by oral dosing of Terminalia catappa fruit extract in doses of 40 mg/kg and 80 mg/kg followed by CFA administration to day 21 and behavioral, hematological parameters besides histopathological screening were assessed simultaneously. Results: The arthritic symptoms were observed to gradually decrease in treatment groups when compared against disease control groups. The parameters like body weight and pain latency were increased whereas paw volume and arthritic score were found to significantly decrease in treatment groups as compared to disease control group. The hematological parameters were also modified and were studied in treatment groups. The histopathological estimation depicted extent of damage in joint tissue of diseased rat comparable to normal, standard and combination therapy group. Conclusion: The fruits possess anti-arthritic properties and significantly reduced paw volume, arthritic score and increased pain threshold and body weight gain besides theory hematological effects like increase in Hb and RBC and decrease in WBC, platelets and ESR levels simultaneously. The histopathological estimation confirmed decreased deterioration of joint tissue in treatment groups as compared to disease control group hence proving to be an emerging therapeutic option in management of RA.

scholarly journals Effects of oxyclozanide (Tremacid®) preparation against fascioliasis on clinical and haematological parameters in cattle of Bangladesh

2015 ◽  
Vol 3 (1) ◽  
pp. 46 ◽  
Author(s):  
Naim Alam ◽  
Md Islam ◽  
Matiar Howlader ◽  
Nasrin Lucky
Keyword(s):  
Body Weight ◽  
Untreated Control ◽  
Hematological Parameters ◽  
Experimental Period ◽  
The Body ◽  
Haematological Parameters ◽  
Total Leukocyte Count ◽  
Control Group ◽  
Untreated Control Group ◽  
Group B

<p>The effects of Oxyclozanide (Tremacid®) on some clinical (body weight) and haematological parameters (TEC, Hb, PCV, ESR and TLC) were determined in this study. Among 55 cattle, 10 were selected, which were suffering from fascioliasis and divided randomly into two groups. Each group was consisting of five cattle. One was treated with Tremacid® @ (15 mg/kg body weight). Other was kept as an infected control group. Before trials with Tremacid® initial body weight, EPG of liver fluke and hematological parameters were examined. During the experimental period the faecal samples, clinical and hematological parameters were examined on 7th, 14th, 21st and 28th days for the determination of effects of Tremacid®. A significant reduction of EPG count was found on 7th, 14th, 21st and 28th day of Tremacid® (61.87%, 71.22%, 76.98% and 84.53%) in a treated cattle group. The EPG of an untreated control group was significantly (p&lt;0.01) increased about 3.97%, 7.64%, 11.04% and 64.89% respectively. Total TEC was increased after treatment with Tremacid® and decreased in an untreated control group significantly (p&lt;0.01), Likewise, after treatment with Tremacid® Hb content and PCV were increased, and ESR was decreased. Total leukocyte count (TLC) was decreased and the body weight was increased after Tremacid® treatment in the group A. On the other hand, Hb content PCV and body weight was decreased significantly (p&lt;0.01) in the group B.</p>

scholarly journals Evaluation of Effect of Melatonin on Hematological Parameters in Patients of Periodontitis

2019 ◽  
pp. 46-49
Author(s):  
Anagha Marawar ◽  
Pramod Marawar ◽  
Nandal D H ◽  
Rahul Kunkulol ◽  
Sandeep Narwane
Keyword(s):  
Leukocyte Count ◽  
Hematological Parameters ◽  
Total Leukocyte Count ◽  
Differential Leukocyte Count ◽  
Erythrocyte Sedimentation ◽  
Significant Difference ◽  
Group A ◽  
Group B ◽  
Root Planning ◽  
Scaling And Root Planning

Introduction: Considering the immunity enhancing property of melatonin, a study on the evaluation of the effect of melatonin on the hematological parameters in patients suffering from Periodontitis.  Methods: This study was conducted in the Department of Periodontics, Rural Dental College, Loni. Patients of chronic Periodontitis, of age between 18 to 65 years of either gender ready to give informed consent to participate in the study were included. Postoperative patients, patients having night duties, drivers and those using heavy machinery, pregnant women, lactating mothers, patients with any clinically significant systemic disease and patients on any other drugs were excluded from the study. Patients were divided into two Groups. Group B received scaling and root planning (SRP) and melatonin 3 mg per day were compared to Group A which received SRP only. The Total Leukocyte count, Differential Leukocyte count and Erythrocyte sedimentation rate of each patient was studied at a baseline, 1st (30 days) visit, 2nd (60 days) visit and 3rd (90 days) visit. Results: Both the groups consisted of 80 subjects each. Group A consisted of 71.25% male and 28.75% female patients. There were 56.25% male and 43.75% female patients in Group B. With respect to visit 3, the TLC was statistically lower in Group B. The neutrophil count of Group B was significantly lower during visit 3 as compared to Group A. The lymphocyte count of Group B was significantly lower than that of Group A during the visit 1, and also significantly higher during visit 3 on comparison with Group A. There was no statistically significant difference in the groups with respect to eosinophil and basophil count. The Monocyte count of Group B was statistically lower when compared to that of Group A. There was no statistically significant difference between ESR of Group A & Group B.  Conclusions: It can be concluded that melatonin has a positive effect on TLC and differential count of patients of periodontitis, conferring a new facet to the management of periodontitis and an attempt to impede the disease progression. Keywords: Melatonin; Hematological parameters; Periodontitis; Total Leukocyte count; Differential Leukocyte count; Erythrocyte sedimentation rate; Scaling and Root Planning.

scholarly journals Responses of Fluvalinate Induced Stress in Hematological and Biochemical Parameters of Lake van Fish (Alburnus tarichi guldenstadt 1814)

2001 ◽  
Vol 71 (3) ◽  
pp. 528-533
Author(s):  
Necati Ozok
Keyword(s):  
Leukocyte Count ◽  
Serum Glucose ◽  
Total Leukocyte Count ◽  
Control Group ◽  
Lake Van ◽  
Serum Enzyme ◽  
Lactate Dehydrogenase Activity ◽  
Glucose Levels ◽  
Induced Stress ◽  
Hematological And Biochemical Parameters

This research was conducted to determine the toxicity of fluvalinate to Lake Van fish (Alburnus tarichi) biometric, hematologic, and serum biochemicals. The fish were exposed to sub lethal fluvalinate concentration for 24, 48, 72 and 96 h for hematological and biochemical analyzes. Red blood cell, hemoglobin and hematocrit values decreased significantly in fish exposed to fluvalinate compared to control group. Significant increases in total leukocyte count were found. Compared with the control group, the serum enzyme aspartate aminotransferase, alanine aminotransferase, lactate dehydrogenase activity and serum glucose levels have increased significantly. This synthetic pyrethroid can be said to have a toxic effect on the Lake Van fish and can be used for controlling toxicity.

UJI AKTIVITAS ANALGETIK EKSTRAK ETANOL HERBA SEMBUKAN (Paederia foetida L.) PADA MENCIT PUTIH JANTAN (Mus musculus) YANG DIINDUKSI DENGAN ASAM ASETAT

2020 ◽  
Vol 5 (2) ◽  
pp. 358-363
Author(s):  
Triswanto Sentat ◽  
◽  
Fitri Handayani ◽  
Ellen Indraswari
Keyword(s):  
Body Weight ◽  
Analgesic Activity ◽  
Mus Musculus ◽  
Ethanol Extract ◽  
Optimal Dose ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Treatment Groups ◽  
Paederia Foetida

Sembukan plants (Paederia foetida L.) are wild vines that are usually used by people as potential medicinal plants for pain. The purpose of this study was to determine the ethanol extract of sembukan’s herbal analgesic activity in male white mice (Mus musculus) and determine the optimal dose of the ethanol extract from sembukan’s herbal (Paederia foetida L.) which has the potential as an analgesic. Sembukan’s herbal is extracted with 70% ethanol solvent and an analgesic activity test is divided into 5 treatment groups namely positive control group (potassium diclofenac), negative control, dose I (80 mg / kg body weight), dose II (160 mg / kg body weight) ) and dose III (320 mg / kg body weight) by oral administration. Thirty minutes after administration, the mice were given an indicator of pain, 0.5% acetic acid. Analgesic power is calculated by counting the amount of stretching of mice for 1 hour. From the results of the study, the ethanol extract of sembukan’s herbal has analgesic activity in male white mice with percent dose analgesic power 35.18%, dose II 53.58% and dose III 68.98%. The optimal dose that has potential as an analgesic in male white mice is dose III with 68.98% analgesic power.

scholarly journals Effects of garlic, turmeric and betel leaf against gastrointestinal nematodes in cattle

1970 ◽  
Vol 6 (1) ◽  
pp. 115-119 ◽  
Author(s):  
MR Amin ◽  
M Mostofa ◽  
MA Awal ◽  
MA Sultana
Keyword(s):  
Body Weight ◽  
Biochemical Parameters ◽  
Leukocyte Count ◽  
Water Extract ◽  
Gastrointestinal Nematodes ◽  
The Other ◽  
Total Leukocyte Count ◽  
Control Group ◽  
Betel Leaf ◽  
Group D

The experiment was conducted in the Department of Pharmacology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, for a period of 28 days to study the effects of garlic (Allium sativum, Linn., @100mg/kg bwt.), turmeric (Curcuma longa, Linn., @100mg/kg bwt.)  and betel leaf (Piper betle, Linn., @100mg/kg bwt.) against natural gastrointestinal nematodes in cattle. The effects of garlic, turmeric and betel leaf on some hematological parameters (TEC, Hb, PCV and TLC), biochemical parameters (ALT and AST) and clinical parameter (body weight) were also observed. Twenty four (24) naturally parasitized cattle of BAU Dairy Farm, Mymensingh were randomly divided into four groups, each consisting of six (6) cattle. Water extract of bulbs of garlic were administered orally to the cattle of group A. Cattle of group B received orally water extract of rhizome of turmeric. Cattle of group C were treated orally with leaves of betel leaf. Cattle of group D was kept as infected control group. Fecal samples, body weight, hematological and biochemical parameters were examined before treatment and on 3rd, 10th, 17th and 28th day. A significant (p < 0.01) reduction of EPG count was found following administration of garlic (20.41-40.81%), turmeric (6.09-19.27%) and betel leaf (2.91-9.8%) in cattle. The EPG count of the control group (D) were significantly (p < 0.01) increased up to the last day of experimental period. After treatment with garlic and turmeric total erythrocyte count (TEC), hemoglobin (Hb) content and packed cell volume (PCV) were gradually increased significantly (p < 0.01 and p < 0.05) in cattle. Conversely, the total leukocyte count (TLC) were decreased significantly (p < 0.01 and p < 0.05) in treated cattle. On the other hand, TEC, Hb content and PCV were gradually increased significantly (p < 0.01 and p < 0.05) on day 3 and day 10 in betel leaf treated cattle but decreased on 17th and 28th day. Conversely, the total leukocyte count (TLC) were decreased significantly (p < 0.01 and p < 0.05) on day 3 and day 10 in betel leaf treated cattle but increased significantly (p < 0.01) on 17th and 28th day. The alanine aminotransferase (ALT) and aspartate aminotransferase (AST) level were not significantly changed in the cattle. The body weight was increased significantly (p < 0.01 and p < 0.05) in garlic, turmeric and betel leaf treated cattle. On the other hand, body weight was decreased in untreated control group. The present study reveals that water extracts of garlic were moderately effective and turmeric and betel leaf were relatively less effective against gastrointestinal nematodes in cattle. Key words: Nematodes, garlic, turmeric, betel, leaf, cattle DOI = 10.3329/bjvm.v6i1.1349 Bangl. J. Vet. Med. (2008). 6 (1): 115-119

scholarly journals The effects of iron and vitamin B12 on body weight and hematobiochemical parameters in mice

2019 ◽  
Author(s):  
M. N. Hasan ◽  
M. S. Jahan ◽  
M. S. Kamal ◽  
M. F. Islam ◽  
S. Ahmed ◽  
...  
Keyword(s):  
Body Weight ◽  
Vitamin B12 ◽  
Biochemical Parameters ◽  
Hematological Parameters ◽  
Animal Health ◽  
Research Work ◽  
Total Leukocyte Count ◽  
Control Group ◽  
Hematobiochemical Parameters ◽  
Group B

Background: Vitamin and mineral supplementation is common practice in both human and animal health, But indiscriminate, overdose and long term used may cause toxicity. The research work was designed to evaluate the effect of iron (Fe) &vitamin B12 (Vit-B12) on body weight, hematological parameters: Total Erythrocyte Count (TEC), Hemoglobin (Hb) and Total Leukocyte Count (TLC), and biochemical parameters: serum creatinine (SC), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels in mice. Methods: One-month old 20 female Swiss Albino mice (Rattus norvegicus) having 19.52±0.54 g body weight were divided into four equal groups (n=5). After acclimatization, the mice of group A was treated with Fe@ 0.2 mg/kg body weight (bwt) in pellet, group B was treated with Vit-B12 @ 5 mg/kg bwt, group C was treated with combination of Fe and Vit-B12 and group D was fed with standard pellet and was considered as control. Results: The result showed significant (P<0.05) increase in body weight of treatment groups compared to the control group. The values of TEC, Hb and TLC in the treated groups were increased significantly (P<0.05). ALT, AST and SC values were decreased significantly (P<0.05) in the treated groups compared to the control group. Conclusions: It is concluded that administration of Fe and Vit-B12 significantly (P<0.05) increased body weight and hematological (TEC, Hb, and TLC) parameters however biochemical parameters (ALT and AST) were decreased significantly (P<0.05). In addition, combination of Fe&Vit-B12 showed better effects compared to their single uses.

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