scholarly journals Association between Malondialdehyde and Glutathione (L-gamma-Glutamyl-Cysteinyl-Glycine/GSH) Levels on Workers Exposed to Benzene in Indonesia

2019 ◽  
Vol 7 (7) ◽  
pp. 1198-1202 ◽  
Author(s):  
Abdul Rohim Tualeka ◽  
Tri Martiana ◽  
Ahsan Ahsan ◽  
Syamsiar S. Russeng ◽  
Wulan Meidikayanti
Keyword(s):  
Free Radicals ◽  
Free Radical Oxidation ◽  
Thiobarbituric Acid ◽  
The Body ◽  
Radical Oxidation ◽  
Benzene Concentration ◽  
Flame Ionisation Detector ◽  
Glutathione Enzymes ◽  
The Impact ◽  
Detoxification Process

BACKGROUND: Chemicals that enter the body, especially benzene, will undergo a detoxification process. Unfortunately, at the detoxification process, sometimes benzene can produce free radicals. Free radical oxidation of lipids produces MDA compounds (malondialdehyde). To overcome these free radicals, the body will adapt to produce Glutathione (GSH) enzymes. AIM: The purpose of this study was to analyse the relationship between benzene concentration, MDA levels and glutathione enzymes in Shoe-Maker Home Industry workers exposed to benzene for more than 10 years. METHODS: Measurement of benzene concentration using a gas chromatography-flame ionisation detector (GC-FID). MDA levels used a modified spectrophotometric and GSH method of thiobarbituric acid (TBA) test. RESULT: The results showed that the majority of respondents had benzene concentrations still below the TLV value, mean of MDA levels were 6.94 mg/ml, while GSH was 4.54 mg/ml. Benzene concentration did not have a significant correlation with MDA and glutathione levels, whereas MDA levels had a strong correlation with glutathione levels (p = 0.000; r = -0.947). CONCLUSION: Workers should always use PPE and always eat foods that contain lots of glutathione enzymes such as spinach or broccoli to reduce the impact of free radicals from benzene inhalation.

scholarly journals Evaluation of the adaptogenic propertries of the Quark product enriched with probiotics, polyphenols and vitamins

10.5219/1156 ◽  
2019 ◽  
Vol 13 (1) ◽  
pp. 713-719 ◽  
Author(s):  
Zinaida Zobkova ◽  
Liliy Fedulova ◽  
Tatyana Fursova ◽  
Daria Zenina ◽  
Elena Kotenkova
Keyword(s):  
Magnetic Field ◽  
Blood Serum ◽  
Dairy Product ◽  
Low Frequency ◽  
Free Radical Oxidation ◽  
Variable Magnetic Field ◽  
The Body ◽  
Radical Oxidation ◽  
And Control ◽  
The Impact

The aim of the study is to evaluate protective properties of the quark product manufactured with transglutaminase and enriched with probiotics, oligomerous proanthocyanidines and vitamins; the biological experiment on the growing laboratory Wistar stock rats has been carried out. The rats of two from three groups subjected within 21 days to the effect of low-frequency weak variable magnetic field received in semi-synthetic diet composition extra experimental and control samples of the quark product. The index of feed intake and the rats’ body mass growth was registered within 32 days. At the end of the experiment blood serum biochemical index was evaluated. It was revealed that the animals consuming the experimental product substantially gained the mass before the effect (gain from the 1st up to 10th days made up 12%) as well as after effect (gain from 11th up to 32nd days – 10.3%); upon completion of the experiment the gains of these animals exceeded the gains of the rats consuming the control product by more than 28%. The experiment revealed the lipolipedemic and hypoglycemic effect of the experimental quark product that has been evidenced by the significant reduction of cholesterol (by more than 20%), glucose (up to 40%) in the rats’ blood serum. On administration of the experimental dairy product in the animals’ diet subjected to the impact of low-frequency weak magnetic field the effect of the broken balance recovery in antioxidant/pro-oxidant system was observed due to reduction of pro-oxidant load at the enzymatic as well as low molecular links of the antioxidant system. The identified antioxidant and adaptogenic effect of the developed dairy product promoting to reduce the intensity of free-radical oxidation at the impact of low-frequency electromagnetic field on the body make it possible recommend it in dietotherapy for correction of antioxidant/pro-oxidant status.

scholarly journals UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL DAUN BUAS-BUAS (Premna serratifolia L.) ASAL KABUPATEN MELAWI PROVINSI KALIMANTAN BARAT DENGAN METODE DPPH

2020 ◽  
Vol 3 (2) ◽  
pp. 405-412
Author(s):  
Weni Puspita ◽  
◽  
Dina Yuspita Sari ◽  
Ika Ristia Rahman ◽  
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Free Radical ◽  
Cell Damage ◽  
Antioxidant Properties ◽  
Free Radical Oxidation ◽  
The Body ◽  
Radical Oxidation ◽  
Ic50 Values ◽  
Ethanol Extracts

Buas (Premna serratifolia L.) contains secondary metabolites of flavonoids, saponins, tannins and triterpenoids/steroids, where flavonoids are known to have antioxidant properties and counteract free radicals. Antioxidants are compounds that can fight toxic hazards and reduce cell damage to the body caused by free radical oxidation. The purpose of this study was to determine the antioxidant activity of ethanol extracts of buas-buas leaves was carried out by DPPH (2,2-diphenyl-1-picrylhydrazyl), using UV-Vis spectrophotometry at wavelength 518.40 nm with various concentrations of 10 ppm, 20 ppm, 30 ppm, 40 ppm and 50 ppm. The results showed that Buas Buas leaves (Premna serratifolia L.) had an antioxidant activity with IC50 values ??of 20.66 ug / mL.

Gender differences in biochemical measurement reflecting the state of free-radical oxidation and antioxidant protection among workers in metallurgical production

2019 ◽  
Vol 1 (10) ◽  
pp. 877-881
Author(s):  
I. A. Umnyagina ◽  
L. A. Strakhova ◽  
T. V. Blinova
Keyword(s):  
Oxidative Stress ◽  
Gender Differences ◽  
Free Radical ◽  
Biochemical Parameters ◽  
Free Radical Oxidation ◽  
The Body ◽  
Antioxidant Protection ◽  
Radical Oxidation ◽  
Metallurgical Production ◽  
Production Factors

Introduction. To date, age and sex differences have been established for many biochemical parameters. Gender differences in indicators for systems such as antioxidant, thiol-disulfide, oxidative stress and inflammation systems are absent or under study.The aim of the study was to identify gender differences in biochemical parameters reflecting the functioning of antioxidant systems of the body and free radical oxidation in workers of metallurgical production, in contact with harmful production factors.Materials and methods. The blood of men and women working at the metallurgical enterprise of the Nizhny Novgorod region (n=80) under the influence of a complex of physical and chemical production factors was studied. Total oxidative stress, total antioxidant capacity of serum, glutathione levels were studied by photometric biochemical methods. Levels of C-reactive protein and 8-hydroxy–2-deoxyguanosine were studied by ELISA.Results. The average amount of peroxides in the serum of women exceeded 1.6 times this figure in men. In the group of men, the content of 8-Ondg was higher by 26% (p=0.012), the level of GS-by 12% (p=0.019), the activity of SOD — by 1.5–2 times (p=0.0001), the level of CRP — by 2 times (p=0.008) compared to similar indicators in women.Conclusions. Studies of gender differences in workers under the influence of harmful production factors will allow more effective approach to the etiology, treatment and prognosis of production-related diseases. Indicators of oxidative stress and antioxidant protection can be indicators of the health of workers under the influence of harmful industrial factors and be important in the prevention of diseases associated with oxidative stress.

Assessment of the Biochemical Status of Workers Manufacturing Ethylbenzene and Styrene

2020 ◽  
pp. 40-43
Author(s):  
RR Galimova ◽  
ET Valeeva ◽  
GV Timasheva ◽  
AB Bakirov
Keyword(s):  
Catalase Activity ◽  
Free Radical Oxidation ◽  
The Body ◽  
Atherogenic Index ◽  
Radical Oxidation ◽  
Professional Groups ◽  
Biochemical Status ◽  
The Central Nervous System ◽  
Hepatic Protein Synthesis ◽  
Increased Activity

Introduction: Production of ethylbenzene and styrene (EBS) is one of the most important stages in organic synthesis. The products have general toxic, hepatotoxic, irritating and narcotic effects on the human body. Severe exposures to EВS can induce pronounced disorders of the central nervous system such as styrene sickness and encephalopathy and of peripheral blood such as leukopenia and lymphocytosis. Materials and methods: We studied homeostasis indices in 376 workers of the main professional groups engaged in the production of EBS including equipment operators, repairmen, and instrumentation and automation fitters. Results: We established an increase in lipid peroxidation by the level of malondialdehyde amid an increase in catalase activity and a decrease in blood retinol and α-tocopherol levels. We also noted an increased activity of indicator enzymes including ALT, AST, GGT, and alkaline phosphatase. Significant changes in lipid metabolism in the form of cholesterolemia, triglyceridemia, a higher atherogenic index, and lower cholesterol of non-atherogenic blood serum lipids demonstrating atherogenic changes in the body were revealed. Conclusions: The earliest prenosological disorders in the body of the examined workers included an impaired hepatic protein synthesis, the development of cytolysis processes and a change in the integrity and functional activity of the liver cell in individuals, an imbalance in the oxidant-antioxidant system, one of the reasons of which was the adverse occupational exposure to hazardous chemicals. An increase in catalase activity is a protective compensatory reaction during the activation of free radical oxidation processes.

scholarly journals Features of free oxidation in liver homogenates of rats in combined action of copper and subtoxic level of stearate potassium and sodium

2016 ◽  
Author(s):  
O. V. Lototska
Keyword(s):  
Free Radical ◽  
Liver Homogenate ◽  
Sodium Stearate ◽  
Free Radical Oxidation ◽  
The Body ◽  
Antioxidant Systems ◽  
Radical Oxidation ◽  
Combined Action ◽  
Liver Homogenates ◽  
Free Oxidation

Toxic effects of heavy metals on animals and humans is aggravating the presence of surfactants. The aim of our research was to identify the peculiarities of free radical oxidation and antioxidant protection in the use of drinking water with subtoxic doses of potassium and sodium stearates in combination with copper on the body of warmblooded animals, such as the liver of experimental rats. Analysis of indicators allows asserting that in experimental rats liver homogenate observed activation of free radical oxidation, the intensity of which depended on the concentration of stearates in water. More pronounced changes were in animals that consumed water with potassium stearate.The action of potassium stearate observed inhibition of antioxidant systems while under the influence of sodium stearate – its activation. Changes in performance were more pronounced in combination stearates with copper.

scholarly journals The Effect of Hair Spray Exposure to the Changes in Electrical Properties of Mice Organ

2020 ◽  
Vol 9 (3S) ◽  
pp. 354-358
Keyword(s):  
Dielectric Constant ◽  
Free Radicals ◽  
Electrical Properties ◽  
Organ Damage ◽  
The Body ◽  
Oxidation Reactions ◽  
Cell Components ◽  
Organ Tissue ◽  
Complex Polymers ◽  
The Impact

Human activities at this time tend to use substances that are pollutants and have an impact on health. Pollutants can enter the body through the process of respiration and will disrupt the organ function. Substances that are often used in daily life and potentially as pollutants are hairspray. They contain vinyl acetate and methacrylate complex polymers. Cocamide DEA surfactants in hairspray can cause cancer and produce toxins. The use of hairspray continuously will cause organ damage due to the emergence of secondary metabolites and free radicals. The diagnosis of organ damage in the latest research from various scientists is to utilize the electrical characteristics of the organ. The bioelectric characteristic that can be observed is dielectric constant. Based on the impact of organ damage and suspected changes in organ electrical properties due to exposure to pollutants, a study needs to be conducted to observe changes in the resistivity and dielectric constant in organ tissue in mice exposed to hairspray with cocamide DEA surfactant content. The results showed that the more concentration of exposure given causes increasing the level of damage to the organs. The increasing level of damage is very significant in the changing of the resistivity and dielectric constant value of mice organs. The changing of electrical properties of mice organs is possible due to damage to organ cells caused by increasing reactive oxygen species and free radicals due to oxidation reactions resulting from the interaction of cocamide DEA with cell components.

scholarly journals INFLUENCE OF LASER TREATMENT ON INDICATORS OF FREE-RADICAL OXIDATION AND ANTIOXIDANT PROTECTION IN SECONDARY PYELONEPHRITIS

2019 ◽  
Vol 19 (1-2) ◽  
pp. 149-156
Author(s):  
I. I Antipkin ◽  
E. V Ryazantsev ◽  
V. E Ryazantsev ◽  
A. M Khoshnaw
Keyword(s):  
Catalase Activity ◽  
Clinical Medicine ◽  
Malonic Dialdehyde ◽  
Free Radical Oxidation ◽  
The Body ◽  
Complex Treatment ◽  
Metabolic Homeostasis ◽  
Radical Oxidation ◽  
Non Destructive ◽  
Single Kidney

Relevance. Optimization of the conservative tactics of acute inflammation of the kidney in nephrolithiasis makes it advisable to use non-traditional methods of detoxification low-intensity laser radiation (LILR) in particular. Aim. To evaluate the effectiveness of laser therapy (LT) in the complex correction of acute secondary pyelonephritis (ASP) in patients with single kidney stones according to the basic adaptation criteria of oxidative-antioxidant clinical medicine КЛИНИЧЕСКАЯ МЕДИЦИНА homeostasis of the organism. Materials and methods. Indices of endogenous intoxication (EI), characterizing the oxidative-antioxidant homeostasis of the body in ASP, were studied in 96 patients with nephrolithiasis aged 19 to 40 years. Patients of the comparison group (48 people) underwent basic (according to the standards) treatment aimed at controlling of calculous pyelonephritis. Patients of the main group (48 people) in the traditional conservative therapy from the second day of hospitalization daily received additional LT sessions with apparatus LTA “Uzor-3KS” with a frequency of 0.6 kHz, a power of 1.5 watts within 4 minutes (total for treatment course is eight procedures). The effectiveness of therapy was assessed on the 5th and 9th day of the inpatient stay. Results. It was found that endotoxicosis in non-destructive forms of ASP is caused by the increase in the concentration of malonic dialdehyde of plasma (by 20.3%) and erythrocytes (by 35.5%), decrease in catalase activity (by 14.2%) and the amount of total (by 11.8%) and free (21.1%) thiol groups with a decrease in the detoxification index (DI) by 24,1%. Compared with the baseline therapy (BT), the effectiveness of LT in the complex treatment of ASP in the analyzed parameters of metabolic homeostasis against the background of the increase in catalase capacity from 6.9 to 10.2%, the detoxification index from 5 to 12.5% was 7.5% (on the 5th day) and 17.5% (on the 9th day of treatment). Conclusions. ASP leads to activation of ROS, suppression of antioxidant and catalase activity of blood, reduction of DI, indicating a subcompensated syndrome of EI. Standard therapy contributes to the normalization of ROS and SLA values of body protection and reduces the level of endotoxicosis caused by ASP. Additional inclusion in the complex treatment of LT sessions more significantly normalizes redox metabolic homeostasis and EI parameters, improving the effectiveness of basic therapy.

scholarly journals Effects of His-Phe-Arg-Trp-Pro-Gly-Pro peptide on free-radical oxidation processes in conditions of chronic restraint stress

2021 ◽  
Author(s):  
AO Vorvul ◽  
II Bobyntsev ◽  
OA Medvedeva ◽  
IuE Azarova ◽  
AE Belykh ◽  
...  
Keyword(s):  
Free Radical ◽  
Restraint Stress ◽  
Free Radical Oxidation ◽  
Regulatory Peptides ◽  
Thiobarbituric Acid ◽  
Synthetic Analog ◽  
Chronic Restraint Stress ◽  
Radical Oxidation ◽  
Oxidation Processes ◽  
Wide Range

Studying the effects of regulatory peptides on the stress-induced shifts in the bodily processes is of great fundamental and applied significance. Currently, a wide range of peptide neurotropic drugs, affecting the stress response development, are used in medicine, and new promising molecules are being studied. The study was aimed to assess the effects of the adrenocorticotropic hormone (ACTH) synthetic analog, ACTH(6-9)-Pro-Gly-Pro, administered at a dose of 5, 50 and 500 μg/kg, on the free-radical oxidation processes in Wistar rats, subjected to chronic restraint stress (CRS) during two weeks. Serum levels of 8-oxo-2'-deoxyguanosine (8-OHdG) and superoxide dismutase 3 (SOD3) were assessed by enzyme immunoassay, and the levels of thiobarbituric acid reactive substances (TBARS) were assessed by fluorimetric method. CRS lead to the significant increase in the 8-OHdG levels by 18.4% (p = 0.01) and the decrease in the SOD3 levels by 14.3% (p = 0.01), however, it had no effect on the levels of TBARS. ACTH(6-9)-Pro-Gly-Pro, administered at a dose of 5 and 50 μg/kg, significantly decreased the levels of 8-OHdG by 19.8% (p = 0.03) and 30% (p = 0.001), respectively. Thus, it was found that CRS resulted in oxidative stress in animals. ACTH(6-9)-Pro-Gly-Pro administration at a dose of 5 and 50 μg/kg inhibits the stress-induced free-radical oxidation processes.

scholarly journals ANALYSIS OF THE INTERRELATION OF CYTOGENETIC HOMEOSTASIS AND OXIDATIVE STRESS IN THE ORGANISM OF GOBY FISH (GOBIIDAE) OF THE NORTHERN CASPIAN

2018 ◽  
Vol 13 (2) ◽  
pp. 64-72
Author(s):  
Tatiana V. Kuzina ◽  
Maya L. Galaktionova
Keyword(s):  
Lipid Peroxidation ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Micronucleus Test ◽  
Functional Dependence ◽  
Thiobarbituric Acid ◽  
The Body ◽  
Muscle Tissues ◽  
Fish Blood ◽  
The Impact

Aim. The aim is to investigate the relationship between the content of micronuclei, destructive disorders in the nuclei of erythrocytes of peripheral blood of the goby fish and intensity of lipid peroxidation in fish tissues. Methods. Was used the method of quantitative accounting of morphologically altered red blood cells of peripheral blood of goby fish. Evaluation of cytogenetic disorders in fish blood cells was carried out based on a micronucleus test. Samples of muscle tissues and liver were sampled for biochemical analyzes. The rates of lipid peroxidation (LPO level) were determined by a method based on the reaction of malondialdehyde and thiobarbituric acid, the end product of lipid peroxidation. Results. A correlation was found between the rates of spontaneous LPO, ascorbate-dependant LPO and the accumulation of malondialdehyde in the liver of fish and changes in the nucleus of erythrocytes (R2 =0,8; R2 =0,6; R2 =0,7, respectively). Conclusions. We established the functional dependence which justifies the need to use cytogenetic markers to assess the impact of adverse environmental factors on the body of hydrobionts.

scholarly journals Тютюнокуріння: розповсюдженість та вплив на органи і тканини порожнини рота (огляд літератури)

2016 ◽  
Author(s):  
V. V. Shcherba ◽  
O. Ya. Lavrin
Keyword(s):  
Oral Cavity ◽  
Oral Cavity Cancer ◽  
Age Of Onset ◽  
Free Radical Oxidation ◽  
Extensive Experience ◽  
Major Health ◽  
Radical Oxidation ◽  
Increased Risk ◽  
Smoking Duration ◽  
The Impact

<p>Smoking is a global problem of humanity, and its impact on the organs and tissues of the oral cavity is a major health and social problem. Smoking is associated with significantly increased risk of the oral cavity diseases, especially with paradont pathology and cancer. Persons who have extensive experience of smoking, use tobacco products for oral use, except of smoking alcohol abuse belong to risk group of the oral cavity cancer. The severity of periodontal disease depends on the age of onset of smoking, duration of smoking and number of cigaretteswhich are smoked daily. In the literature many papers are devoted to research the impact of smoking on periodontal status. However, a number of aspects, including free radical oxidation state is left without proper attention, which is the aim of our further investigation.</p>

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