scholarly journals The Effect of Follicular-fluid Exposure of Endometriosis Cyst Patients toward the Levels of Bcl-2 and Cytochrome-C in Mice Oocyte

2020 ◽  
Vol 8 (A) ◽  
pp. 567-570
Author(s):  
Ida Bagus Putra Adnyana ◽  
I Made Jawi ◽  
Ketut Suwiyoga ◽  
I Made Bakta ◽  
I Nyoman Mantik Astawa ◽  
...  

BACKGROUND: Endometriosis cysts adversely affect a woman’s quality of life, which causes pain and reduces fertility. The World Health Organization found that the incidence of endometriosis with infertility clinical manifestations is around 10%. AIM: The purpose of this study is to determine the presence of mice oocytes mitochondrial apoptosis exposed with endometriosis cysts follicular fluid through Bcl-2 and cytochrome C analysis. METHODS: This study was a randomized post-test only control group design conducted at Sanglah Hospital, Bali Royal Hospital in Denpasar, and the Medical Faculty Udayana University from June 2018 to April 2019. A total of 120 mice oocytes were distributed randomly into three groups, i.e., treatment Groups 1 and 2 that added by 15% endometriosis cysts follicular fluid and control group. Bcl-2 and Cytochrome C analyzed with ELISA Technique. Comparability was tested with one-way ANOVA with a significance level of p < 0.05. RESULTS: Eighteen mice oocyte replicates were normally distributed and homogeneous. The Bcl-2 levels in the treatment Groups 1, and 2 and control were 627.83 ± 146.42, 634.50 ± 140.62, and 678.83 ± 152.71, respectively; p = 0.838 (not significantly different). Cytochrome C levels in the treatment Groups 1, and 2, and control, respectively, were 3147.75 ± 228.50, 3104.45 ± 211.29, and 2738.28 ± 227.45; p = 0.021 (significantly different). CONCLUSION: The effect of endometriosis cysts follicular fluid exposure on mitochondrial apoptosis is proven through Cytochrome C, whereas Bcl-2 is not proven.

2019 ◽  
Vol 1 (2) ◽  
pp. 226-235
Author(s):  
Afnijar Wahyu ◽  
Liza Wati ◽  
Murad Fajri

The purpose of this study was to determine the effect of AIUEO therapy on the speech ability of stroke patients who have motor aphasia in Raja Ahmad Thabib Hospital Tanjungpinang. The research design used was quasi experiment with the Nonequivalent Control Group Design approach to 9 respondents who were divided into 9 treatment groups and 9 control groups. The results showed that there were significant differences in the functional ability of communication between the control and treatment groups with a value of p <0.05 (p = 0.007 at a = 0.05) using the Wilcoxon Test statistical test. Conclusion, the influence of AIUEO therapy on the speech ability of stroke patients with motor aphasia in the treatment and control groups at Ahmad Thabib Hospital Tanjungpinang.   Keywords: Speech Ability, Motor Aphasia Stroke, AIUEO Therapy


2021 ◽  
Vol 56 (3) ◽  
pp. 186
Author(s):  
Thomas Eduardus Sudrajat Wahyu Nugroho ◽  
Sitti Rizaliyana ◽  
David S Perdanakusuma

Background:Closure of the defect with a random skin flap is constrained by the extent of the defect area. Several studies have shown the benefits of administration of PRP (Platelet Rich Plasma) in random skin flap. The purpose of this study is to compare the effect of PRP injection given before flap elevation, after flap elevation and control on the extended random skin flap procedures in rats.Methods: This research is experimental with randomized post test only control group design (n = 27, divided into 3 treatment groups) that compare the effectiveness of PRP injection 24 hours prior to the elevation of the flap, after the elevation of the flap, and control in the extended random skin flap in rats. Random skin flap is made in the ratio 1: 5 on the rat skin. Measurement of viable area were observed on days 1, 7 and 14.Results: There were significantly increased viability of random skin flap on the group with PRP injection 24 hours prior flap elevation compared to other group of treatment. The average of viability on day first 39%±13% ;42%±34%; 62%±14%. On day 7th24%±13%; 36%±26%; 62%±12%.On day 14th16%±15%; 28%±22%; 60%±11%.Conclusion: Injection of PRP on extended random skin flap on rat 24 hours before flap elevationincrease viability of the flap compared to control group and group which receive PRP injection on extended random flap on rat skin after elevation of the flap. Viability increased due to angiogenesis stimulation after PRP injection.


Author(s):  
Nora Mawinda ◽  
Zulkifli Naansah ◽  
Hanesman Hanesman

The problem in this study is the low learning outcomes achieved by students on the subjects of Basic Electrical and Electronics which is below the minimum completeness criteria (KKM) set a school that is 75 This is presumably because they are learning is often used during the learning model is applied directly proven yet effective. The purpose of this research is to reveal how much influence the Jigsaw cooperative learning model to study the results of class X students on subjects TAV Basic Electricity and Electronics. This research is an experimental study with a draft Post-test only control group design. The sample were students of class X TAV at SMK 1 Padang Academic Year 2014 / 2015. Class experiment is treated using Jigsaw Cooperative Learning Model and the control group is a class that uses the direct teaching model. The data is taken from the test results in the form of learning about the objective as many as 31 items. Data were analyzed manually to test for normality, homogeneity testing, and hypothesis testing. The result of the calculation hypothesis at significance level α = 0.05 was found that t count> t table is 3.35> 1.670. The results of these tests give an interpretation that H0 is rejected and H1 is accepted, this means that on average significantly experimental class learning outcomes greater than the average control class learning outcomes.  Key words :   Models of Learning, Jigsaw, Learning Direct, Post-test only control group design, Learning outcomes, Experimental, and Control.


2021 ◽  
Vol 2126 (1) ◽  
pp. 012013
Author(s):  
P D Lestari ◽  
J Mansyur

Abstract This study aimed to investigate the effect of the online PhET simulation-assisted using the direct instruction on students’ conceptual understanding of the parabolic motion. It was quasi-experimental research with pretest-posttest control group design. The research subjects were 10th grade students, consisting of 20 students for each experimental and control groups. The data were collected using a concept understanding test. The research data were analyzed quantitatively by using descriptive and inferential statistics. The results show that the average score of the experimental class was higher than the control class. The hypothesis was tested using Mann-Whitney (U-Test) with a significance level of = 0.05. Based on data analysis, it can be concluded that there was a significant effect of online PhET simulation assisted learning model on students’ concept understanding on parabolic motion.


2017 ◽  
Vol 2 (3) ◽  
pp. 106
Author(s):  
Ria Rizki Ekasari ◽  
Gunawan Gunawan ◽  
Hairunnisyah Sahidu

This research aims to investigate the influence of direct instruction model with virtual laboratory media toward student creativity in physics using "pretest-posttest control group design". The creativity data collected by essay type test shows that pre-test average grade of experimental class is 41 and control class is 36, while post-test average grade of experimental class is 74 and control class is 69. Based on data analysis, creativity of male student in physics is higher than female student. The average grade of male student is 72 while female student is 70. The result of hypothesis testing using polled variance t-test with significance level of 5 % is tcalculation=> ttable = 1,99601 or, in other words, the calculated t value is greater than the value given in the table. It can be concluded that direct instruction model with virtual laboratory media influences high school student creativity in physics.


2019 ◽  
Vol 9 (2) ◽  
pp. 109
Author(s):  
Husnul Fadila ◽  
Jismulatif Jismulatif

ABSTRACT: This quasi-experimental research aimed to find out whether there is an effect of heritage narrative on reading comprehension of second year students of SMP Telekomunikasi Pekanbaru. This research was a nonequivalent (pretest and posttest) control-group design. In this design, there two groups as the participant (experimental and control group). Heritage narrative was applied in teaching the experimental group while the control group was taught by using narrative from other culture. The population of this research was all students of second year students in academic year of 2017/2018. The total number of population was 71. The sample is chosen by using cluster random sampling. In analyzing data, the writer used the quantitative approach. The results of the data analysis showed that the mean scores of pre-test were 44.86 (experimental group) and 43.47 (control group). The mean score of students after the treatment was higher than pre-test. Moreover, from the statistical analysis, it was found out the t-test (4.75) was higher than t-table (2.01) at the significance level 5%. It can be concluded that there was effect of heritage narrative on students ’reading comprehension.


2021 ◽  
Vol 13 (3) ◽  
pp. 2631-2638
Author(s):  
Ellychristina D. Hutubessy ◽  
Santiah Santiah ◽  
Nastasha Hera Pramesya

This study aims to determine the effect of Lerntabeguch on learning outcomes of German writing skills using the freizeit theme. The method used in this study is a quantitative method with a research design of Pretest Posttest Control Group Design. The research was conducted at State Senior High School 31 Jakarta. The research sample was taken by a simple random sampling technique. Data were collected through documents and writing tests. Validity tests were using Pearson Product Moment and reliability using Cronbach Alpha. While the data analysis technique used a t-test. The results showed that the t-count Pretest in the experimental class and control class was 1.108 with t-table at a significance level of = 0.05 and degrees of freedom (DK) = 34. While the results of the t-count Posttest in the experimental class and control class were 3,485. So, there is an effect of using Lerntagebuch on learning outcomes of German writing skills for class XII students related to the topic of Free Time Activities (Freizeit).


2020 ◽  
Vol 9 (3) ◽  
pp. 314-320
Author(s):  
Indang Retno Fourniyati ◽  
Murbangun Nuswowati ◽  
Edy Cahyono

This study aims to examine the effect of project-based chemistry learning models on cearning completeness and student creativity through the Chemsong video making project. The research method uses the pretest-posttest control group design while the data analysis uses the parametric statistical test that is N-gain, the independent sample t test and the sample t-test paired test. The sample in this study was obtained by cluster random sampling technique from the population of all students of SMK N 8. The gain normality test showed that the average results of the pretest-posttest clasical learning completeness increased by 0.53 with the criteria being in the experimental class and the control class at 0,31 with moderate criteria. The results of the independent sample t-test showed that there was a significant difference in the classical learning completeness of the experimental and control group students, which was 4.56 greater than t critic 1.98 with a significance level of 5%. In the paired sample t test, the value of sig. (2-tailed) of 0,000 <0.05. Whereas the results of student creativity t-test obtained sig values. (2-tailed) of 0,000 <0.05. So it can be concluded that the application of the chemistry learning model based on the Chemsong video project has an effect on improving student mastery learning and creativity.


2020 ◽  
Vol 56 (3) ◽  
pp. 186
Author(s):  
Thomas Eduardus Sudrajat Wahyu Nugroho ◽  
Sitti Rizaliyana ◽  
David S Perdanakusuma

Closure of the defect with a random skin flap is constrained by the extent of the defect area. Several studies have shown the benefits of administration of PRP (Platelet Rich Plasma) in random skin flap. The purpose of this study is to compare the effect of PRP injection given before flap elevation, after flap elevation and control on the extended random skin flap procedures in rats. This was an experimental study with randomized posttest-only control group design (n=27, divided into 3 treatment groups) that compare the effectiveness of PRP injection 24 hours prior to the elevation of the flap, after the elevation of the flap, and control in the extended random skin flap in rats. Random skin flap is made in the ratio 1: 5 on the rat skin. Measurement of viable area were observed on days 1, 7 and 14. There were significantly increased viability of random skin flap on the group with PRP injection 24 hours prior flap elevation compared to other group of treatment. The average of viability on day first 39%±13% ;42%±34%; 62%±14%. On day 7th 24%±13%; 36%±26%; 62%±12%. On day 14th 16%±15%; 28%±22%; 60%±11%. Injection of PRP on extended random skin flap on rat 24 hours before flap elevation increase viability of the flap compared to control group and group which receive PRP injection on extended random flap on rat skin after elevation of the flap. Viability increased due to angiogenesis stimulation after PRP injection.


2016 ◽  
Vol 3 (2) ◽  
pp. 101-106
Author(s):  
Anita Rahmawati

Father’s role strongly proved in increasing the success of breastfeeding process, but in realitymany fathers are still not implementing its role in the process of breastfeeding (breastfeeding father’srole) because of father’s education and the assumption of breastfeeding is mother’s responsibilities. Thepurpose of this study was to analyze the effect of the father prenatal education toward breastfeedingfather’s role. This study used post test only control group design. 30 samples were taken by purposivesampling which further divided into control and treatment groups by simple random. Breastfeedingfather’s role was measured by a questionnaire and the data were analyzed by independent t test. Theresults showed breastfeeding father’s role rate and standard deviation in the treatment group was 15.40± 2.558, while in the control group 9.80 ± 4.212. Different test treatment and control group obtained thevalue p = 0,000 which means there was a significant effect of education toward breastfeeding father’srole between the treatment group and the control group. The nurse recommended to educate pregnantwomen prenatal classes which was devoted to the father (husband) as an alternative solution to improvethe success of exclusive breastfeeding program.


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