scholarly journals A procedure for dual simple t-cannulation in the small intestine of pigs using a single right flank laparotomy

2020 ◽  
Vol 8 (3) ◽  
pp. 135-141
Author(s):  
A.L. Petry ◽  
S.A. Gould ◽  
J.F. Patience
Keyword(s):  
Body Weight ◽  
Small Intestine ◽  
Ileocecal Valve ◽  
Nutrient Digestibility ◽  
Lateral Side ◽  
Initial Body Weight ◽  
Novel Approach ◽  
Experimental Trials ◽  
Gross Examination ◽  
Cannula Placement

Singular cannulation in the small intestine of pigs is a common methodology for studying nutrient digestibility, delivering compounds into the gastrointestinal tract, or repeated tissue and digesta sampling. In that respect, it is an important tool for nutritionists and researchers. However, there is a dearth of detailed methodologies describing multiple intestinal cannulations using modern techniques. The objective of this experiment was to develop a single right flank laparotomy technique that allowed for imsertion of multiple cannulas in the small intestine, with minimal variation, and allowed for externalisation of both cannulas on the same lateral side as the laparotomy. Thirty gilts (L337 × Camborough) with an initial body weight of 30.2±0.78 kg underwent the procedure. Each gilt was equipped with a simple t-cannula in the terminal ileum, approximately 10 cm cranial to the ileocecal valve, and a second t-cannula in the jejunum 240 cm distal from where the duodenum is visually posterior to the transverse colon. The procedure used hallmarks of commonly implemented terminal ileal cannulation techniques, but modified the laparotomy location and cannula externalisation sites and used a novel approach for determining the more proximal cannula location to mitigate the need of a second laparotomy. Gilts were allowed to recover for a minimum of 7 d and were used for an average of 67 days in subsequent experimental trials. Cannula longevity was from 30 to 73 kg of body weight. Pigs were necropsied for surgical site gross examination and small intestine measurements at the end of the experiment. The jejunal cannula had a mean placement of 298.90±9.96 cm distal to the pyloric sphincter with a coefficient of variation of 3.33%. Hence, this procedure provided a single laparotomy technique for obtaining digesta from multiple locations of the small intestine, with minimal variation in proximal cannula placement.

Factorial and discriminant analysis on non-carcass components of Berganês lambs from different sexual classes and crossbreeding

2021 ◽  
pp. 1-11
Author(s):  
José Renaldo Vilar da Silva Filho ◽  
João Bandeira de Moura Neto ◽  
Lays Thayse Alves dos Santos ◽  
Clebson Oliveira Ferreira ◽  
Rafael Torres de Souza Rodrigues ◽  
...  
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Discriminant Analysis ◽  
Multivariate Statistics ◽  
Total Weight ◽  
Initial Body Weight ◽  
Total Weight Gain ◽  
Santa Inês ◽  
Experimental Trials ◽  
By Products

Abstract The aim of this study was to evaluate the non-carcass components (NCC) of Berganês ecotype lambs of different sexual classes and genotypes using univariate and multivariate statistics, carrying out two experimental trials. In order to evaluate the effects of the sexual class, non-castrated males (BNC), castrated males (BC) and females (BF) of Berganês ecotype lambs were used, with mean initial body weight of 27 ± 3.1 kg. To evaluate crossbreeding, non-castrated male lambs of the genotypes Berganês (BG), Berganês × Santa Inês (BSI) and Berganês × Dorper (BD) were used, as well as the control Dorper × Santa Inês (DSI), all with mean initial body weight of 28 ± 3.8 kg. The weight and yield of the total by-products was higher for BNC. Regarding the genotype, BSI showed higher weight and yield of internal fat, but the weight and yield of the total by-products was higher for BG and BD. In factorial analysis (FA), the NCC, more correlated with empty body weight (EBW) and total weight gain (TWG), showed higher eigenvectors for factor 1. For factor 2, the weights and yields of internal fat and total viscera obtained higher eigenvectors. The discriminant analysis (DA) classified 100% of individuals in their respective sexes and genotypes. Therefore, the FA indicated that, among the NCC evaluated, the weights of liver, kidneys, GIT, skin and feets are determinant for obtaining EBW and TWG. The classification achieved by the DA indicates that the sexual classes and genotypes are heterogeneous.

scholarly journals Nutritional Status Evaluation (Estimated Using Lignin Internal Indicator) of Grazed Yearling Kacang Bucks

2015 ◽  
Vol 17 (3) ◽  
pp. 138
Author(s):  
Retno Adiwiniarti ◽  
I Gede Suparta Budisatria ◽  
Kustantinah Kustantinah ◽  
Rusman Rusman ◽  
Agung Purnomoadi
Keyword(s):  
Body Weight ◽  
Nutritional Status ◽  
Dry Matter ◽  
Nutrient Digestibility ◽  
Initial Body Weight ◽  
Central Java ◽  
Internal Indicator ◽  
Set Up ◽  
Internal Marker ◽  
Soccer Fields

In some areas in Indonesia, farmers usually graze their goats during the day and house them at night.  The goats eat grass without additional concentrate.  However, how much nutrient intakes of grazing goats in Indonesia are seldom calculated. Therefore, this research was set up to investigate the productivity and to estimate the dry matter, protein, and TDN intake of grazed Kacang buck using lignin internal indicator.  About 12 heads of Kacang buck, aged 1-1.5 year (the incisors have erupted 1), having the initial body weight of 18.42+1.78 kg (CV= 9.67%) and reared by farmers in Grobogan, Central Java, Indonesia were used in this study.  Intake and nutrient digestibility of the buck were calculated using lignin as internal marker.  After being reared 86 days, the average daily gains (ADG) of the buck were 3.20+21.14 gram ( -26.16 gram to 53.60 gram). Low ADG of the buck was caused by low DMI, protein and TDN intake.  The Kacang bucks were usually grazed in the soccer fields, back yards, and roadsides, therefore their intake were lower than their maintenance needed.  The averages of DMI, protein and TDN intake were about 1.17+0.17 % their body weight, 30.16+4.18 g and 91.45+11.64 g, respectively. It can be concluded that the productivity of grazing Kacang bucks is relatively very low.

scholarly journals Extrusion of soybean hulls does not increase digestibility of amino acids or concentrations of digestible and metabolizable energy when fed to growing pigs

2020 ◽  
Vol 4 (3) ◽  
Author(s):  
Diego A Rodriguez ◽  
Su A Lee ◽  
María R C de Godoy ◽  
Hans H Stein
Keyword(s):  
Body Weight ◽  
Soybean Meal ◽  
Basal Diet ◽  
Nutrient Digestibility ◽  
Growing Pigs ◽  
Metabolizable Energy ◽  
Adaptation Period ◽  
Initial Body Weight ◽  
Soybean Hulls ◽  
Total Dietary Fiber

Abstract Two experiments were conducted to determine effects of extrusion on energy and nutrient digestibility in soybean hulls. One source of soybean hulls was ground and divided into two batches. One batch was used without further processing, whereas the other batch was extruded. In Exp. 1, four diets were formulated to determine crude protein (CP) and amino acid (AA) digestibility in soybean hulls. A soybean meal-based diet in which soybean meal provided all the CP and AA was formulated. Two diets were formulated to contain 30% nonextruded or extruded soybean hulls and 18% soybean meal. An N-free diet that was used to determine the endogenous losses of CP and AA was also used. Eight growing barrows (initial body weight = 37.0 ± 3.9 kg) had a T-cannula installed in the distal ileum and were allotted to a replicated 4 × 4 Latin square design. Each experimental period lasted 7 d with the initial 5 d being the adaptation period and ileal digesta were collected for 8 h on day 6 and 7. Results indicated that extrusion of soybean hulls did not change the standardized ileal digestibility (SID) of CP and most AA with the exception that the SID of Ile and Leu tended (P < 0.10) to be greater in extruded than nonextruded soybean hulls. In Exp. 2, three diets were formulated to determine energy digestibility in soybean hulls. One corn-soybean meal based basal diet, and two diets that contained corn, soybean meal, and 32% extruded or nonextruded soybean hulls were formulated. Twenty-four growing barrows (initial body weight = 59.9 ± 3.4 kg) were allotted to a randomized complete block design. Pigs were housed individually in metabolism crates and feces and urine were collected separately for 4 d after 5 d of adaptation. The apparent total tract digestibility (ATTD) of gross energy (GE) and the digestible energy (DE) and metabolizable energy (ME) were reduced (P < 0.05) in diets containing nonextruded or extruded soybean hulls compared with the basal diet. However, the ATTD of GE and values for DE and ME in soybean hulls were not improved by extrusion. Likewise, extrusion did not change the concentration of total dietary fiber in soybean hulls. In conclusion, there were no effects of extrusion of soybean hulls on SID of AA, energy digestibility, or ME concentration in soybean hulls.

scholarly journals Dietary Supplementation with Flammulina velutipes Stem Waste on Growth Performance, Fecal Short Chain Fatty Acids and Serum Profile in Weaned Piglets

Animals ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 82 ◽  
Author(s):  
Xuzhou Liu ◽  
Jinbiao Zhao ◽  
Gang Zhang ◽  
Jiangxu Hu ◽  
Ling Liu ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Body Weight ◽  
Growth Performance ◽  
Basal Diet ◽  
Short Chain Fatty Acids ◽  
Nutrient Digestibility ◽  
Weaned Piglets ◽  
Initial Body Weight ◽  
Animal Trial ◽  
Dietary Treatments

This study was conducted to evaluate the effects of dietary FVS supplementation on growth performance, nutrient digestibility, biochemical profile of serum and fecal short chain fatty acids (SCFAs) production in weaned piglets. In Exp.1, 150 weaned pigs (initial body weight: 6.89 ± 1.17 kg) were allotted to five dietary treatments. The treatment diets included a basal diet and four experimental diets supplemented with 2.5%, 5.0%, 7.5% and 10.0% FVS respectively. The animal trial lasted for 28 days. In Exp.2, 72 piglets (initial body weight: 8.20 ± 1.67 kg) were allotted to three dietary treatments. The treatment diets included a basal diet and two experimental diets supplemented with 1.5% and 3.0% FVS, respectively. The animal trial lasted for 56 days. The results showed that pigs fed dietary FVS with 3% or lower inclusion levels had no significant difference (p > 0.10) on growth performance compared with pigs fed the control diet during day 1–28 and day 1–56. Dietary FVS supplementation decreased the apparent total tract digestibility (ATTD) of nutrients on day 28, day 35 and day 56, but no significant changes (p > 0.05) of nutrient digestibility were observed on day 14. Although piglets fed diets with higher levels of FVS showed impaired growth performance and ATTD of nutrients, dietary FVS supplementation improved the fecal SCFA production, antioxidant capacity, interleukin-2 and growth hormone levels in serum, and reduced the harmful low-density lipoprotein levels in serum on day 56. In conclusion, as a promising alternative fibrous ingredient, FVS could be supplemented in diets of weaned piglets with a proportion under 3%.

Effect of lecithin with or without chitooligosaccharide on the growth performance, nutrient digestibility, blood metabolites and pork quality of finishing pigs

2008 ◽  
Vol 88 (2) ◽  
pp. 283-292 ◽  
Author(s):  
Won-Tae Kim ◽  
Prashant Shinde ◽  
Byung Jo Chae
Keyword(s):  
Body Weight ◽  
Growth Performance ◽  
Average Daily Gain ◽  
Serum Triglyceride ◽  
Nutrient Digestibility ◽  
Blood Metabolites ◽  
Pork Quality ◽  
Finishing Pigs ◽  
Initial Body Weight

Two experiments were conducted to evaluate the effect of dietary lecithin with or without chitooligosaccharide (COS) on the performance, blood metabolites, pork cholesterol, fatty acid composition and quality of finishing pigs. In exp. 1, 36 pigs (Landrace × Yorkshire × Duroc, 84.5 ± 0.60 kg initial body weight) were fed lecithin at 0, 2.5 or 5.0% of the diet. Lecithin improved average daily gain (16%) and feed conversion ratio, and did not affect apparent nutrient digestibility. On day 28, lecithin decreased serum total and low density lipoprotein (LDL) cholesterol (34 and 77%, P = 0.016), and increased serum triglyceride (P = 0.048). Lecithin did not affect carcass characteristics and pork quality, but increased myristic and α-linolenic acid and reduced palmitoleic acid in pork. Experiment 2 involved 108 pigs (85.0 ± 0.76 kg initial body weight) in a 2 × 2 factorial arrangement of treatments, wherein two levels of lecithin (low, 2.5 and high, 5.0%) and COS (0.0 and 0.1%) were used. Addition of COS in diets containing lecithin reduced pork cholesterol (16.4%) and oleic acid (28.3%), and did not affect performance, nutrient digestibility, blood metabolites and pork quality. In conclusion, these results suggest that lecithin improved the growth performance of finishing pigs and inclusion of COS reduced the amount of cholesterol in pork. Key words: Lecithin, COS, performance, nutrient digestibility, pork quality

scholarly journals Determination of Maintenance Energy Requirements for Fattening Castrated Korean Black Goats (Capra hircus coreanae)

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1543
Author(s):  
Sang-Ho Moon ◽  
Yeong Sik Yun ◽  
Na Yeon Kim ◽  
Sanguk Chung ◽  
Qi Man Zhang ◽  
...  
Keyword(s):  
Body Weight ◽  
Energy Intake ◽  
Energy Levels ◽  
Average Daily Gain ◽  
Nutrient Digestibility ◽  
Metabolic Energy ◽  
Energy Requirements ◽  
Capra Hircus ◽  
Maintenance Energy ◽  
Daily Gain

Twelve adult (10 months old) castrated Korean black goats, with an average initial body weight of 24.98 ± 3.7 kg, were used in this experiment to determine their maintenance energy requirements. Dry matter intakes (g/d, p = 0.945) were not affected by energy levels, but metabolic energy intake (kcal/d, p < 0.002) and average daily gain (g/d, p < 0.001) were significantly increased at higher energy levels. Nutrient digestibility was similar in the treatments, but crude fat digestibility increased with the addition of protective fat powder (p = 0.001). The energy required for fattening the castrated Korean black goats was estimated using the correlation between metabolic energy intake per dietary body weight and average daily gain per dietary body weight. The Y-axis intercept value was calculated to be 108.76 kcal/kg BW0.75 (p < 0.05, r2 = 0.6036), which was the metabolic energy requirement for maintaining the lives of the fattening Korean black goats. The estimated energy requirements of the black goat can improve specification techniques, such as the energy level and the amount of feed supply required for domestic black goats.

PSV-19 Effect of Low Nutrient Density Diet with Probiotic Mixture (B. Subtilis ms1, B. Licheniformis SF5-1, and S. Cerevisiae) Supplementation on Performance of Weaner Pigs

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 193-195
Author(s):  
Vetriselvi Sampath ◽  
Hyun Ju Park ◽  
Yong min Kim ◽  
Je Min Ahn ◽  
Inho Kim
Keyword(s):  
Body Weight ◽  
Growth Performance ◽  
Average Daily Gain ◽  
Nutrient Digestibility ◽  
The Body ◽  
Interactive Effects ◽  
Low Density ◽  
Gas Emission ◽  
Nutrient Density ◽  
Weaner Pigs

Abstract BACKGROUND: A total of one hundred and forty, 28 d-old weaner pigs [Duroc x (Yorkshire x Landrace)] with initial body weight (BW) of 6.56±1.25kg were used in a six-week treatment (7 replicate pens per treatment; barrows, and 2 gilts/pen) to evaluate the effect of low nutrient density diet supplement with probiotic mixture supplementation on the growth performance, nutrient digestibility, fecal microbial, and gas emission of weaner pigs. RESULTS: Pigs fed low-density diet with probiotic mixture supplementation had linearly increased (P = 0.028, 0.014) the body weight (BW) at weeks 3, and 6. Moreover, average daily gain (ADG) was linearly improved (P=0.018, 0.014, 0.014) at week 3, 6, and overall experiment. However, there were no interactive effects found on the nutrient digestibility of dry matter (DM), nitrogen (N) and energy (E) throughout the experiment. Dietary inclusion of low-density diet with probiotic mixture supplementation has improved the fecal lactobacillus counts linearly, but E. coli was unaffected during the trail. On day 42, Ammonium gas emission was significantly decrease in pigs fed a low-density diet with probiotic mixture supplementation. However, H2S, acetic acid, and CO2 were not significantly affected by the probiotic mixture supplementation diet. CONCLUSION: Low-density diet with probiotic mixture supplementation had positively affected the growth performance, fecal microbial, and fecal gas emission on weaner pigs.

scholarly journals Effects of Defatted Rice Bran Inclusion Level on Nutrient Digestibility and Growth Performance of Different Body Weight Pigs

Animals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1374
Author(s):  
Bingbing Huang ◽  
Huangwei Shi ◽  
Li Wang ◽  
Lu Wang ◽  
Zhiqian Lyu ◽  
...  
Keyword(s):  
Body Weight ◽  
Growth Performance ◽  
Rice Bran ◽  
Nutrient Digestibility ◽  
Growing Pigs ◽  
Neutral Detergent Fiber ◽  
Control Group ◽  
Defatted Rice Bran ◽  
Gross Energy ◽  
Stage 1

This study was conducted to determine the effects of low-protein diet prepared with different levels of defatted rice bran (DFRB) and weight stages on growth performance and nutrient digestibility of growing–finishing pigs. The animal experiment included three stages. A total of 240 growing pigs with an initial body weight of 28.06 ± 8.56 kg for stage 1 were allocated to five diets including one control group and four DFRB diets supplemented with 2.5%, 5%, 7.5% and 10% DFRB, respectively. The 192 crossbred pigs with initial body weights of 55.03 ± 7.31 kg and 74.55 ± 9.10 kg were selected for stage 2 and stage 3, respectively. Pigs were allocated to four diets including one control group and three DFRB diets supplemented with 10%, 15% and 20% DFRB, respectively. The results showed that with the increase in DFEB intake, the gain: feed was linearly increased (p < 0.05), and the average daily feed intake tended to linearly decrease (p = 0.06) in stage 1. Except for the apparent total tract digestibility (ATTD) of acid detergent fiber (ADF) in stage 3, levels of DFRB had significant effects on the ATTD of gross energy (GE), dry matter (DM), ash, neutral detergent fiber (NDF) and ADF in three weight stages. In stage 1, with the increase in levels of DFRB, the ATTD of NDF and hemicellulose were firstly increased and then decreased (p < 0.01). In stage 2, with the increasing levels of DFRB, the ATTD of DM, ash and cellulose were firstly increased and then decreased (p < 0.01). In stage 3, the ATTD of GE, DM, ash, NDF and hemicellulose decreased linearly with the increase in levels of DFRB (p < 0.01). Collectively, DFRB could be used as a replacement for corns and soybean meal, and weight stage is important to consider when adjusting the additive proportion.

Studies on Eimeria grenieri in the guinea fowl (Numida meleagris)

Parasitology ◽  
1978 ◽  
Vol 76 (1) ◽  
pp. 1-9 ◽  
Author(s):  
P. L. Long ◽  
B. J. Millard
Keyword(s):  
Drinking Water ◽  
Body Weight ◽  
Small Intestine ◽  
Body Weight Gain ◽  
Chorioallantoic Membrane ◽  
Severe Depression ◽  
Guinea Fowl ◽  
Third Generation ◽  
Numida Meleagris ◽  
Pathogenic Effects

SummaryEimeria grenieri was isolated from intensively reared guinea fowl in Britain. The oocysts had average dimensions of 21·09 × 15·48 μm and a small micropyle. Three generations of schizonts were found in the small intestine. The first mature schizonts were found in the upper intestine 30 h after inoculation. Second-generation schizonts were seen 48–80 h and third-generation schizonts were present 80–96 h after inoculation. These schizonts were found in the middle and lower small intestine. It was confirmed that gametogony of this species occurs only in the caeca. The pre-patent time was 112 h and the reproductive index from a dose of 6 × 102 oocysts was 8 × 104. Doses of between 1 × 104 and 1 × 106 caused severe depression of body weight gain. Immunity to re-infection developed rapidly. Sulphaquinoxaline at a dose of 0·04% in the drinking water given after inoculation was effective in reducing the pathogenic effects of the disease. Robenidine given in the food at a concentration of 16·5 ppm (50% of the recommended concentration for chickens) was effective in preventing disease caused by E. grenieri.The endogenous life-cycle of E. grenieri was completed in the chorioallantoic membrane of developing chicken embryos.

scholarly journals The Influence of Broilers’ Body Weight on the Efficiency of Electrical Stunning and Meat Quality under Field Conditions

Animals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1362
Author(s):  
Giorgio Smaldone ◽  
Stefano Capezzuto ◽  
Rosa Luisa Ambrosio ◽  
Maria Francesca Peruzy ◽  
Raffaele Marrone ◽  
...  
Keyword(s):  
European Union ◽  
Body Weight ◽  
Meat Quality ◽  
Well Being ◽  
Electrical Equipment ◽  
Post Mortem ◽  
The European Union ◽  
Body Weights ◽  
Corneal Reflex ◽  
Experimental Trials

Water-bath stunning represents the most-applied stunning system in poultry slaughtering, but within the European Union, specific indications on electric parameters that should be used, such as voltage, are missing. The objective of this study was to evaluate the efficiency of two commercially available types of electrical equipment (A and B) on broilers with different live body weights and the influence of the tested parameters on meat quality. Experimental trials in a European Union-approved slaughterhouse were carried out using two different stunners. 6600 broilers, divided into three weight groups, were stunned applying different protocols based on the same current frequencies and intensity but different voltages. The state of unconsciousness (presence of corneal reflex and wings flapping) and post-mortem defects (pectoral hemorrhages and dark meat) were evaluated by blinded trained operators. The presence of corneal reflex and petechiae were the most reported consciousness signs and post-mortem injuries, respectively. Different weights played an important role within stunner A, registering statistical differences (p < 0.01) among groups. Considering injuries, an inverse relationship between body weight and lesions was found. The results highlighted the effectiveness of both stunning systems applying the best combination of electrical parameters considering the weight of the animal and ensuring its well-being.

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