Exposure of laying hens to mycotoxins through naturally contaminated feed
The objective of the present study was to assess the degree of exposure of laying hens to mycotoxins through naturally contaminated feed and the hygienic quality of feeds. For this purpose, the natural mycoflora and the occurrence of aflatoxins and fumonisins were evaluated in 95 feed samples intended for laying hens collected from January to December 2010 at the Experimental Farm of the State University of Londrina, Northern Paraná State, Brazil. Fusarium was the prevalent genus (99%), followed by Aspergillus (89%). Fusarium spp. were detected at a low contamination level (<103 to <104 colony forming units (cfu)/g) in 54% of the samples. Aspergillus spp. were detected in 73% of the samples at the same contamination range. The total mould and yeast count ranged from 5.0×102 to 8.7×105 cfu/g; in 60% of the samples the values exceeded 1.0×104 cfu/g, the maximum limit established to ensure good hygienic quality of the product. Aflatoxins and fumonisins were detected in 69.7 and 89.5% of the samples, with mean values of 9.61 ng/g and 1.28 µg/g, respectively. The estimated daily intake of fumonisin B1 for laying hens (0.038 mg/kg body weight/day) was below the lowest observed adverse effect level (2 mg/kg body weight/ day). Regarding aflatoxins, most of the positive samples (85.1%) showed aflatoxin levels below the maximum level laid down by the European Commission. Nevertheless, continuous monitoring of mycotoxin levels in laying hen feed is essential to minimize threats to human and animal health. To our knowledge, this is the first report on the degree of exposure of laying hens to mycotoxins through naturally contaminated feed in Brazil.