The Inductive Role of Vitamin C and its Mode of Application on Growth, Water Status, Antioxidant Enzyme Activities and Protein Patterns of Vicia faba L. cv. Hassawi Grown under Seawater Irrigation

2008 ◽  
Vol 4 (1) ◽  
pp. 38-51 ◽  
Author(s):  
Mohamed M. Azooz ◽  
Mohamed A Al-Fredan
Keyword(s):  
Vitamin C ◽  
Antioxidant Enzyme ◽  
Enzyme Activities ◽  
Water Status ◽  
Antioxidant Enzyme Activities ◽  
Protein Patterns ◽  
Mode Of Application ◽  
Vicia Faba L ◽  
Seawater Irrigation

scholarly journals Physiological and Antioxidant Parameters in Two Lycoris Species as Influenced by Water Deficit Stress

HortScience ◽  
2015 ◽  
Vol 50 (11) ◽  
pp. 1702-1708 ◽  
Author(s):  
Sheng Xu ◽  
Mingmin Jiang ◽  
Jiangyan Fu ◽  
Lijian Liang ◽  
Bing Xia ◽  
...  
Keyword(s):  
Antioxidant Enzyme ◽  
Water Deficit ◽  
Enzyme Activities ◽  
Water Status ◽  
Sugar Content ◽  
Soluble Sugar ◽  
Net Photosynthesis ◽  
Water Deficit Stress ◽  
Antioxidant Enzyme Activities ◽  
Guaiacol Peroxidase

From a field experiment, the changes in morphophysiological characters and antioxidant enzyme activities were studied in two Lycoris species (Lycoris radiata and Lycoris aurea) subjected to 16 days of water deficit stress. With the increase of water deficit stress processing time, leaf relative water content (RWC), membrane stability index (MSI), net photosynthesis (Pn), stomatal conductance (gS), transpiration rate (E), and chlorophyll (Chl) content decreased in both studied species. The water use efficiency (WUE) showed an opposite tendency between the two species under water deficit stress, where WUE of L. aurea decreased moderately and WUE of L. aurea increased somehow. Intercellular CO2 concentration (Ci) in L. aurea and L. radiata decreased in respond to water deficit stress at early stages of stress treatment, then increased throughout the rest of the stress period, and reached levels higher than those in well-watered plants at the end of the treatment. In addition, there was a significant increment in soluble sugar content and proline accumulation under water deficit stress in both species, and L. radiata showed a much more accumulation. The activity of superoxide dismutase (SOD), guaiacol peroxidase (POD), and ascorbate peroxidase (APX) increased in both plants subjected to water deficit stress while declined as the stress time increased. In L. aurea, catalase (CAT) showed a sustained increment, but it responded later and after a transient increase declined again in L. radiata under water deficit stress. In conclusion, L. radiata was more tolerant to water deficit stress than L. aurea as evidenced by its relatively higher water status, higher levels of proline, soluble sugar and pigments, and stronger photoprotection. Moreover, relatively higher antioxidant enzyme activities and lower levels of thiobarbituric acid reactive substances (TBARS) in L. radiata were also associated with its better protection against water deficit stress-induced oxidative damage.

scholarly journals Anti-Oxidant Effects of Silybum marianum Extracts on Diabetic Wistar Rats

2019 ◽  
Vol 5 (4) ◽  
pp. 145-150
Author(s):  
Uyovwiesevwa Ataihire Johnson ◽  
◽  
Eze Kingsley Nwangwa ◽  
John Chukwuka Igweh ◽  
◽  
...  
Keyword(s):  
Vitamin C ◽  
Antioxidant Enzyme ◽  
Enzyme Activities ◽  
Combined Treatment ◽  
Treated Group ◽  
Control Group ◽  
Antioxidant Enzyme Activities ◽  
Silybum Marianum ◽  
Group I ◽  
Anti Oxidant

Antioxidants are specialized macro-molecules that neutralize harmful substances; free radicals. These radicals supposedly harm tissues, destroy food items, and damage materials. In living organisms, antioxidants can take the form of enzymes, and may be regularly added to oils, metals, foodstuffs, as well as numerous other materials to mitigate the damaging effect of free radical. Current study was designed to investigate the biochemical changes in antioxidant enzyme activities, following administration of Silybum marianum (an ancient medicinal plant of the Carduus marianum family) on Alloxan-Induced, diabetic rats. One hundred and twenty-five (125) rats were procured, made to acclimatize for two weeks, and then randomly grouped into five (5) groups of (n=25). Group 1: Non-Diabetic (Control) rats, Group 2 diabetic untreated rats, while groups 3, 4 and 5 comprised of vitamin-C treated rats (diabetic), Silymarin (extract), and Vitamin C + Silymarin (extract) combined treatment respectively. After four weeks of treatment with test extract, animals were then sacrificed, and blood samples collected and assayed for biochemical [anti-oxidant] enzyme activity. Upon statistical analysis, one way Analysis of variance (ANOVA) showed Catalase (CAT), superoxide dismutase (SOD) and malonaldehyde (MDA) activities to have significantly decreased for extract + vitamin C treated group (Group V) when compared with control (Group I). It was also noted that the use of the combined antioxidants vitamin C and silymarin resulted in a significant reduction in ROS production with decreased SOD and CAT enzyme activities. It is therefore likely that, improvements in antioxidant enzyme activities are a function of extract and/or Vitamin C administration to animals. Thus, Silymarin has antioxidant and regenerative potentials to damaged tissues.

scholarly journals Effects of Propolis on Selected Blood Indicators and Antioxidant Enzyme Activities in Broilers under Heat Stress

2009 ◽  
Vol 78 (1) ◽  
pp. 75-83 ◽  
Author(s):  
Pınar Tatlı Seven ◽  
Seval Yılmaz ◽  
Ismail Seven ◽  
Ibrahim H. Cercı ◽  
Mehmet A. Azman ◽  
...  
Keyword(s):  
Heat Stress ◽  
Vitamin C ◽  
Antioxidant Enzyme ◽  
Enzyme Activities ◽  
Basal Diet ◽  
Positive Control ◽  
Diet Group ◽  
Control Group ◽  
Antioxidant Enzyme Activities ◽  
Group I

In this study, we investigated the antioxidant activity of ethanol extracts of propolis (EEP) and vitamin C on biochemical indicators and antioxidant enzyme activities of broilers exposed to heat stress (at 34 °C). The experimental groups were as follows: group I (positive control) and group II (control) were fed a basal diet, group III (vitamin C) was fed a basal diet supplemented with 250 mg vitamin C as ascorbic acid/kg, group IV (EEP-0.5) was fed a basal diet supplemented with 0.5 g EEP/kg, group V (EEP-1) was fed a basal diet supplemented with 1 g EEP/kg, group VI (EEP-3) was fed a basal diet supplemented with 3 g EEP/kg. Plasma superoxide dismutase levels of positive control, control, vitamin C, EEP-0.5, EEP-1 and EEP-3 groups were found as 0.34, 1.23, 0.50, 0.90, 0.30 and 0.41 μkat/ml, respectively (p < 0.01). Aspartate transaminase (except for EEP-0.5 and EEP-1 groups) and alkaline phosphatase in the control group were significantly higher than those of positive control, vitamin-C and EEP-3 groups. Malondialdehyde level in plasma, liver and muscle tissues of control group were found significantly (p < 0.05) higher than those of positive control and EEP-3 groups. Catalase activities of blood, liver, kidney and heart were the highest in the control group. Reduced glutathione activities of plasma and liver of all groups were not significantly different from each other, whereas those of muscle, kidney and heart were significantly higher in the control group. Significantly lower levels of glutathione peroxidase were found in blood, liver and kidney tissues of the control group (p < 0.05), whereas those of muscle and heart were similar in all groups. The results of the present study suggest that EEP and specially EEP at the supplemented dose of 3 mg/kg diet might be considered to prevent oxidative stress in the broilers exposed to heat stress.

Salt stress differently affects growth, water status and antioxidant enzyme activities in Solanum lycopersicum and its wild relative Solanum chilense

2014 ◽  
Vol 62 (5) ◽  
pp. 359 ◽  
Author(s):  
Juan Pablo Martínez ◽  
Alejandro Antúnez ◽  
Héctor Araya ◽  
Ricardo Pertuzé ◽  
Lida Fuentes ◽  
...  
Keyword(s):  
Salt Stress ◽  
Antioxidant Enzyme ◽  
Solanum Lycopersicum ◽  
Enzyme Activities ◽  
Water Status ◽  
Dry Weight ◽  
Saline Stress ◽  
Antioxidant Enzyme Activities ◽  
Solanum Chilense ◽  
Leaf Succulence

The effect of saline stress (NaCl, 40, 80 and 160 mmol L−1 of NaCl) on growth, plant water status and leaf antioxidant enzyme activities was investigated in a commercial cultivar of cherry tomato (Solanum lycopersicum var. cerasiforme L.) and in a wild-related species collected in a salt-affected area of North Chile (Solanum chilense Dun.). Salt stress was applied in a nutrient solution at the vegetative stage during 40 days. The highest NaCl concentration reduced shoot relative growth, fresh and dry weight and leaf area in the cultivated S. lycopersicum but had less impact on S. chilense. Both species were able to efficiently perform osmotic adjustment but S. chilense also exhibited an increase in leaf succulence. The oxidative stress estimated through malondialdehyde quantification was always higher in the cultivated S. lycopersicum, both in the absence and in the presence of salt. Total superoxide dismutase activity (EC 1.15.1.1) increased in response to the highest dose of NaCl in S. chilense but remained constant in S. lycopersicum. Salinity induced an increase in ascorbate peroxidase (EC 1.11.1.11) in S. chilense but reduced it in S. lycopersicum. It is concluded that S. chilense displays efficient strategies to cope with high NaCl doses and that management of the oxidative status is a key mechanism allowing this species to tolerate salinity.

Alterations in antioxidant enzyme activities and oxidative damage in alcoholic rat tissues: Protective role of Thespesia populnea

2012 ◽  
Vol 132 (1) ◽  
pp. 150-159 ◽  
Author(s):  
Sahitya Chetan Pandanaboina ◽  
Shanmugam Ramudu Kondeti ◽  
Sangeetha Lakshmi Rajbanshi ◽  
Pavan Nagavenkata Kunala ◽  
Saritha Pandanaboina ◽  
...  
Keyword(s):  
Oxidative Damage ◽  
Antioxidant Enzyme ◽  
Enzyme Activities ◽  
Protective Role ◽  
Antioxidant Enzyme Activities ◽  
Rat Tissues ◽  
Thespesia Populnea
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