PREPARATION, CHARACTERIZATION AND DRUG DELIVERY BEHAVIOR OF DEXAMETHASONE-LOADED OLIBANUM MICROSPHERE

2018 ◽  
Vol 30 (05) ◽  
pp. 1850031
Author(s):  
Parastoo Namdarian ◽  
Ali Zamanian ◽  
Azadeh Asefnejad ◽  
Maryam Saeidifar
Keyword(s):  
Tissue Engineering ◽  
Drug Carrier ◽  
Good Choice ◽  
Polymer Materials ◽  
Bioactive Molecules ◽  
Release Mechanism ◽  
Natural Polymers ◽  
Tissue Engineering Scaffolds ◽  
Active Research ◽  
Drug Carrier System

In recent years, the strategy of using microspheres as drug carrier system has been very much considered. Also the use of natural polymers for pharmaceutical applications is very attractive. Given that various polymer materials are available for making microspheres, there is an active research to develop new, safe and effective release for microspheres. Olibanum is used as a drug carrier in this study. Also dexamethasone (DEX) is one of the commonly bioactive molecules used in bone tissue engineering. In this research, the purification and characterization of olibanum were first studied by FTIR, XRD, pH determination and MTT test. Due to its non-toxicity, biocompatibility, availability and biodegradability, this material can be a good choice as a natural polymer. Then the microspheres were synthesized with single emulsion method and studied by SEM and FTIR. The morphology and structure of the microspheres revealed that they are spherical and separate, which have rough and porous surface. Also the release mechanism of drug from olibanum microspheres was determined in accordance with the different kinetic drug release models. Investigating the drugs release behavior and degradation rate of microspheres revealed that they were suitable for dexamethasone-controlled release and can be used well in tissue engineering scaffolds.

Engineered Biomimetic Nanofibers for Regenerative Medicine

2010 ◽  
Vol 76 ◽  
pp. 114-124
Author(s):  
Seeram Ramakrishna ◽  
Jayarama Reddy Venugopal ◽  
Susan Liao
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Regenerative Medicine ◽  
Cardiac Tissue ◽  
Three Dimensional ◽  
Electrospun Nanofibers ◽  
Cost Effective ◽  
Bioactive Molecules ◽  
Natural Polymers ◽  
Hematopoietic Stem

Attempts have been made to fabricate nanofibrous scaffolds to mimic the chemical composition and structural properties of extracellular matrix (ECM) for tissue/organ regeneration. Nanofibers with various patterns have been successfully produced from synthetic and natural polymers through a relatively simple technique of electrospinning. The resulting patterns can mimic some of the diverse tissue-specific orientation and three-dimensional (3D) fibrous structure. Studies on cell-nanofiber interactions have revealed the importance of nanotopography on cell adhesion, proliferation and differentiation. Our recent data showed that hematopoietic stem cells (HSCs) as well as mesenchymal stem cells (MSCs) can rapidly and effectively attached to the functionalized nanofibers. Mineralized 3D nanofibrous scaffold with bone marrow derived MSCs has been applied for bone tissue engineering. The use of injectable nanofibers for cardiac tissue engineering applications is attractive as they allow for the encapsulation of cardiomyocytes/MSCs as well as bioactive molecules for the repair of myocardial infarction. Duplicate 3D heart helix microstructure by the nanofibrous cardiac patch might provide functional support for infarcted myocardium. Furthermore, clinical applications of electrospun nanofibers for regenerative medicine are highly feasible due to the ease and flexibility of fabrication with the cost-effective method of making nanofibers.

scholarly journals Biomimetic Hybrid Systems for Tissue Engineering

Biomimetics ◽  
2020 ◽  
Vol 5 (4) ◽  
pp. 49
Author(s):  
Omid Yousefzade ◽  
Ramaz Katsarava ◽  
Jordi Puiggalí
Keyword(s):  
Tissue Engineering ◽  
Hybrid Systems ◽  
Bioactive Molecules ◽  
Natural Polymers ◽  
Biological Scaffolds ◽  
Structural Support ◽  
Biomimetic Scaffolds ◽  
Complex Preparation ◽  
Cell Growth And Proliferation ◽  
Preparation Techniques

Tissue engineering approaches appear nowadays highly promising for the regeneration of injured/diseased tissues. Biomimetic scaffolds are continuously been developed to act as structural support for cell growth and proliferation as well as for the delivery of cells able to be differentiated, and also of bioactive molecules like growth factors and even signaling cues. The current research concerns materials employed to develop biological scaffolds with improved features as well as complex preparation techniques. In this work, hybrid systems based on natural polymers are discussed and the efforts focused to provide new polymers able to mimic proteins and DNA are extensively explained. Progress on the scaffold fabrication technique is mentioned, those processes based on solution and melt electrospinning or even on their combination being mainly discussed. Selection of the appropriate hybrid technology becomes vital to get optimal architecture to reasonably accomplish the final applications. Representative examples of the recent possibilities on tissue regeneration are finally given.

Formulation, Characterization and In vitro Drug Delivery of Vitexin Loaded Liposomes

2021 ◽  
Vol 14 (2) ◽  
pp. 5364-5371
Author(s):  
Dr.S.Bhagavathy Sivathanu ◽  
Shivapriya G ◽  
Shivapriya G
Keyword(s):  
Drug Delivery ◽  
Drug Carrier ◽  
Egg Yolk ◽  
Drug Carriers ◽  
Good Choice ◽  
Target Site ◽  
Carrier System ◽  
Liposome Preparation ◽  
Drug Carrier System ◽  
Novel Drug

Liposome is a spherical vesicle which contains atleast one lipid bilayer. Liposomes are used as a novel drug carriers because of its hydrophobic and hydrophilic nature, it has many advantages in the field of medical sciences. There are some other drug carriers like dendrimers, micelles, niosomes. Out of all, liposomes are considered to be the most promising agent for drug delivery. The uniqueness of liposome is when it is used as a pharmaceutical drug, it acts as a natural receptor. Thus it acts as an antigen and binds with the antibody (cancer cell) without causing any damage to the adjacent cells. For the synthesis of liposomes, a phospholipid is required. The liposomes can be synthesized using egg yolk and chloroform. So the basic phospholipid is obtained from egg yolk. For more stability, the liposomes are prepared using popc. The present work  discuss about the effective preparation of drug loaded liposomes using popc (1- palmitoyl-2-oleoyl-sn-glycero-3- phosphocholine). POPC is an important phospholipid for biophysical experiments. Additionally chloroform is used as the solvent for the liposome preparation. The drug chosen for liposome loading is vitexin (vxn), which is an effective therapeutic agent against inflammation and cancer. The vesicular size, shape, drug entrapment efficacy, stability, electrochemical property and drug releasing property of the formulated liposomes were characterized. The results showed that the formulated liposomes are considered as the better drug carrier system and good choice for biotransformation within the cell to reach the target site such as cancer cells. Even though available treatments like chemotherapy and radiation therapy, causes damage to the surrounding cells, the alternative drug transferring system such as liposomal mediated drug transfer within the cell is considered as good choice of treatment to avoid such complications. The aim of liposome mediated  drug carrier system is to develop a method to reach the drug to the target site. After drug delivery at the target site, the liposomes are fused within the surface of the body. This is because of the pH of liposomes, which is at 7.4 and temperature is maintained at 37 oC. So, the vxn loaded liposomes are considered as the novel drug carriers for the successful targetted drug delivery.

Preparation and characterization of alginate/HACC/oyster shell powder biocomposite scaffolds for potential bone tissue engineering applications

RSC Advances ◽  
2016 ◽  
Vol 6 (42) ◽  
pp. 35577-35588 ◽  
Author(s):  
Tai-ying Chen ◽  
Hao-chao Huang ◽  
Jia-lin Cao ◽  
Yan-jiao Xin ◽  
Wen-feng Luo ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Repair ◽  
Oyster Shell ◽  
Natural Polymers ◽  
Tissue Engineering Scaffolds ◽  
Repair Materials ◽  
Oyster Shell Powder ◽  
Bone Repair Materials ◽  
Shell Powder

Tissue engineering scaffolds combining biominerals and natural polymers are prospective candidates for bone repair materials.

scholarly journals Novel synthesis strategies for natural polymer and composite biomaterials as potential scaffolds for tissue engineering

2010 ◽  
Vol 368 (1917) ◽  
pp. 1981-1997 ◽  
Author(s):  
Hsu-Feng Ko ◽  
Charles Sfeir ◽  
Prashant N. Kumta
Keyword(s):  
Tissue Engineering ◽  
Low Cost ◽  
Three Dimensional ◽  
Natural Polymer ◽  
Natural Polymers ◽  
Porous Network ◽  
Tissue Engineering Scaffolds ◽  
Composite Scaffolds ◽  
Polymeric Scaffold ◽  
Recent Developments

Recent developments in tissue engineering approaches frequently revolve around the use of three-dimensional scaffolds to function as the template for cellular activities to repair, rebuild and regenerate damaged or lost tissues. While there are several biomaterials to select as three-dimensional scaffolds, it is generally agreed that a biomaterial to be used in tissue engineering needs to possess certain material characteristics such as biocompatibility, suitable surface chemistry, interconnected porosity, desired mechanical properties and biodegradability. The use of naturally derived polymers as three-dimensional scaffolds has been gaining widespread attention owing to their favourable attributes of biocompatibility, low cost and ease of processing. This paper discusses the synthesis of various polysaccharide-based, naturally derived polymers, and the potential of using these biomaterials to serve as tissue engineering three-dimensional scaffolds is also evaluated. In this study, naturally derived polymers, specifically cellulose, chitosan, alginate and agarose, and their composites, are examined. Single-component scaffolds of plain cellulose, plain chitosan and plain alginate as well as composite scaffolds of cellulose–alginate, cellulose–agarose, cellulose–chitosan, chitosan–alginate and chitosan–agarose are synthesized, and their suitability as tissue engineering scaffolds is assessed. It is shown that naturally derived polymers in the form of hydrogels can be synthesized, and the lyophilization technique is used to synthesize various composites comprising these natural polymers. The composite scaffolds appear to be sponge-like after lyophilization. Scanning electron microscopy is used to demonstrate the formation of an interconnected porous network within the polymeric scaffold following lyophilization. It is also established that HeLa cells attach and proliferate well on scaffolds of cellulose, chitosan or alginate. The synthesis protocols reported in this study can therefore be used to manufacture naturally derived polymer-based scaffolds as potential biomaterials for various tissue engineering applications.

scholarly journals Smart Hydrogels in Tissue Engineering and Regenerative Medicine

Materials ◽  
2019 ◽  
Vol 12 (20) ◽  
pp. 3323 ◽  
Author(s):  
Somasundar Mantha ◽  
Sangeeth Pillai ◽  
Parisa Khayambashi ◽  
Akshaya Upadhyay ◽  
Yuli Zhang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Regenerative Medicine ◽  
3D Structure ◽  
Cell Immobilization ◽  
Decisive Role ◽  
High Water ◽  
Bioactive Molecules ◽  
High Water Content ◽  
Growth Factor Delivery ◽  
Tissue Engineering Scaffolds

The field of regenerative medicine has tremendous potential for improved treatment outcomes and has been stimulated by advances made in bioengineering over the last few decades. The strategies of engineering tissues and assembling functional constructs that are capable of restoring, retaining, and revitalizing lost tissues and organs have impacted the whole spectrum of medicine and health care. Techniques to combine biomimetic materials, cells, and bioactive molecules play a decisive role in promoting the regeneration of damaged tissues or as therapeutic systems. Hydrogels have been used as one of the most common tissue engineering scaffolds over the past two decades due to their ability to maintain a distinct 3D structure, to provide mechanical support for the cells in the engineered tissues, and to simulate the native extracellular matrix. The high water content of hydrogels can provide an ideal environment for cell survival, and structure which mimics the native tissues. Hydrogel systems have been serving as a supportive matrix for cell immobilization and growth factor delivery. This review outlines a brief description of the properties, structure, synthesis and fabrication methods, applications, and future perspectives of smart hydrogels in tissue engineering.

scholarly journals Chitosan and Its Potential Use as a Scaffold for Tissue Engineering in Regenerative Medicine

2015 ◽  
Vol 2015 ◽  
pp. 1-15 ◽  
Author(s):  
Martin Rodríguez-Vázquez ◽  
Brenda Vega-Ruiz ◽  
Rodrigo Ramos-Zúñiga ◽  
Daniel Alexander Saldaña-Koppel ◽  
Luis Fernando Quiñones-Olvera
Keyword(s):  
Tissue Engineering ◽  
Regenerative Medicine ◽  
Biomedical Applications ◽  
Therapeutic Strategy ◽  
Biological Properties ◽  
Natural Polymers ◽  
Tissue Engineering Scaffolds ◽  
Promising Alternative ◽  
Functional Biomaterials ◽  
Potential Use

Tissue engineering is an important therapeutic strategy to be used in regenerative medicine in the present and in the future. Functional biomaterials research is focused on the development and improvement of scaffolding, which can be used to repair or regenerate an organ or tissue. Scaffolds are one of the crucial factors for tissue engineering. Scaffolds consisting of natural polymers have recently been developed more quickly and have gained more popularity. These include chitosan, a copolymer derived from the alkaline deacetylation of chitin. Expectations for use of these scaffolds are increasing as the knowledge regarding their chemical and biological properties expands, and new biomedical applications are investigated. Due to their different biological properties such as being biocompatible, biodegradable, and bioactive, they have given the pattern for use in tissue engineering for repair and/or regeneration of different tissues including skin, bone, cartilage, nerves, liver, and muscle. In this review, we focus on the intrinsic properties offered by chitosan and its use in tissue engineering, considering it as a promising alternative for regenerative medicine as a bioactive polymer.

Natural Polymers: Tissue Engineering Scaffolds

2016 ◽  
pp. 5648-5657
Author(s):  
Douglas Ramos Marques ◽  
Tiago Moreno Volkmer ◽  
Luis Alberto dos Santos
Keyword(s):  
Tissue Engineering ◽  
Natural Polymers ◽  
Tissue Engineering Scaffolds

scholarly journals Recent Progress of Fabrication of Cell Scaffold by Electrospinning Technique for Articular Cartilage Tissue Engineering

2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Yingge Zhou ◽  
Joanna Chyu ◽  
Mimi Zumwalt
Keyword(s):  
Tissue Engineering ◽  
3D Structure ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Natural Polymers ◽  
Tissue Engineering Scaffolds ◽  
Electrospinning Technique ◽  
3D Scaffolds ◽  
Cell Growth And Differentiation ◽  
Material Transportation

As a versatile nanofiber manufacturing technique, electrospinning has been widely employed for the fabrication of tissue engineering scaffolds. Since the structure of natural extracellular matrices varies substantially in different tissues, there has been growing awareness of the fact that the hierarchical 3D structure of scaffolds may affect intercellular interactions, material transportation, fluid flow, environmental stimulation, and so forth. Physical blending of the synthetic and natural polymers to form composite materials better mimics the composition and mechanical properties of natural tissues. Scaffolds with element gradient, such as growth factor gradient, have demonstrated good potentials to promote heterogeneous cell growth and differentiation. Compared to 2D scaffolds with limited thicknesses, 3D scaffolds have superior cell differentiation and development rate. The objective of this review paper is to review and discuss the recent trends of electrospinning strategies for cartilage tissue engineering, particularly the biomimetic, gradient, and 3D scaffolds, along with future prospects of potential clinical applications.

Novel tissue engineering scaffolds as wound dressings loaded with Alkannins/Shikonins as active ingredients

2019 ◽  
Author(s):  
AS Arampatzis ◽  
K Theodoridis ◽  
E Aggelidou ◽  
KN Kontogiannopoulos ◽  
I Tsivintzelis ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Wound Dressings ◽  
Active Ingredients ◽  
Tissue Engineering Scaffolds
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