Seed discontinuous hydration does not benefit germination, but improves drought tolerance of Triplaris gardneriana seedlings

The discontinuous seed hydration enables the acquisition of tolerance to environmental stresses, causing a stress imprint. It may modify metabolic patterns and lead to improved stress responses. This study aims to evaluate the effects of discontinuous hydration on germination and on seedling growth of Triplaris gardneriana Wedd. under intermittent drought. The seeds have gone through cycles (0, 1, 2 and 3) of hydration and dehydration (HD). The seedlings produced were subjected to water deficit (daily watering and intervals of seven and fourteen days between watering). Seed germinability parameters and, relative growth rate (RGR) of seedling, leaf area, dry matter yield and leaf relative water content (RWC) were evaluated. The HD cycles did not benefit germination, but two HD cycles induced a better biomass accumulation and increased leaf area in seedlings under moderate water deficit, while three HD cycles promoted an increase in RGR and influenced the RWC values. Severe stress affects seedling growth, but subjection to HD cycles minimizes the deleterious effects of drought, suggesting discontinuous hydration acts leading stress imprint in plants.

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Relationships between physiological traits and productivity of sugarcane in response to water deficit

The Journal of Agricultural Science ◽

10.1017/s0021859612000834 ◽

2012 ◽

Vol 152 (1) ◽

pp. 104-118 ◽

Cited By ~ 16

Author(s):

M. DE A. SILVA ◽

J. L. JIFON ◽

J. A. G. DA SILVA ◽

C. M. DOS SANTOS ◽

V. SHARMA

Keyword(s):

Water Deficit ◽

Field Studies ◽

Water Deficit Stress ◽

Chlorophyll Contents ◽

Physiological Variables ◽

Drought Tolerant ◽

Relative Water ◽

Leaf Relative Water Content ◽

Sugarcane Productivity ◽

Response To Water

SUMMARYThe relationships between physiological variables and sugarcane productivity under water deficit conditions were investigated in field studies during 2005 and 2006 in Weslaco, Texas, USA. A total of 78 genotypes and two commercial varieties were studied, one of which was drought-tolerant (TCP93-4245) and the other drought-sensitive (TCP87-3388). All genotypes were subjected to two irrigation regimes: a control well-watered treatment (wet) and a moderate water-deficit stress (dry) treatment for a period of 90 days. Maximum quantum efficiency of photosystem II (Fv/Fm), estimated chlorophyll content (SPAD index), leaf temperature (LT), leaf relative water content (RWC) and productivity were measured. The productivity of all genotypes was, on average, affected negatively; however, certain genotypes did not suffer significant reduction. Under water deficit, the productivity of the genotypes was positively and significantly correlated with Fv/Fm, SPAD index and RWC, while LT had a negative correlation. These findings suggest that genotypes exhibiting traits of high RWC values, high chlorophyll contents and high photosynthetic radiation use efficiency under low moisture availability should be targeted for selection and variety development in programmes aimed at improving sugarcane for drought prone environments.

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Phenology, leaf gas exchange, growth, and seed yield in contrasting Medicago truncatula and Medicago laciniata populations during prolonged water deficit and recovery

Botany ◽

10.1139/b11-093 ◽

2012 ◽

Vol 90 (2) ◽

pp. 79-91 ◽

Cited By ~ 2

Author(s):

Nasreddine Yousfi ◽

Ines Slama ◽

Chedly Abdelly

Keyword(s):

Water Deficit ◽

Medicago Truncatula ◽

Seed Yield ◽

Growth Parameters ◽

Leaf Gas Exchange ◽

Seed Mass ◽

Field Capacity ◽

Water Shortage ◽

Relative Water ◽

Leaf Relative Water Content

The aims of this study were to investigate the effect of prolonged water stress and recovery on phenology, growth, and seed yield in Tunisian contrasting populations of Medicago truncatula and Medicago laciniata . After ample irrigation for 24 days, the plants of each population were divided into two lots: the first lot was irrigated at 100% field capacity (FC), and the second at only 45% FC. After 24 days of treatment, one lot of dehydrated plants was rewatered at 100% FC, while the other was maintained at 45% FC. Interspecific and intraspecific differences were found in phenological responses to water deficit. All growth parameters were more reduced in M. truncatula populations than in M. laciniata populations. The water shortage tolerance of M. laciniata populations was associated with a lower metabolic impairment of photosynthesis and maintenance of relatively higher leaf relative water content. Seed yield was also more reduced in M. truncatula populations compared with M. laciniata populations. In M. laciniata, seed mass was a compensation mechanism to sustain seed yield under drought conditions. Seed yield variation between populations under water deficit was explained mainly by variation in seed number per plant.

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Physiological responses of two halophytic grass species under drought stress environment

Acta Botanica Croatica ◽

10.1515/botcro-2016-0018 ◽

2016 ◽

Vol 75 (1) ◽

pp. 31-38 ◽

Cited By ~ 9

Author(s):

Zamin Shaheed Siddiqui ◽

Huda Shahid ◽

Jung-Il Cho ◽

Sung-Han Park ◽

Tae-Hun Ryu ◽

...

Keyword(s):

Drought Stress ◽

Stomatal Conductance ◽

Water Deficit ◽

Physiological Responses ◽

Biomass Accumulation ◽

Photosynthetic Performance ◽

Grass Species ◽

Free Proline ◽

Relative Water ◽

Halophytic Grasses

AbstractThe physiological responses of two halophytic grass species, Halopyrum mucronatum (L.) Staph. and Cenchrus ciliaris (L.), under drought stress were evaluated. Biomass accumulation, relative water content, free proline, H2O2content, stomatal conductance, photosynthetic performance and quantum yield (Fv/Fmratio) were studied. Under drought conditions, these halophytic plants expressed differential responses to water deficit. Stomatal conductance and free proline content were higher in H. mucronatum than in C. ciliaris, while H2O2content in H. mucronatum was substantially lower than in C. ciliaris. Performance index showed considerable sensitivity to a water deficit condition, more so in C. ciliaris than in H. mucronatum. Results were discussed in relation to comparative physiological performance and antioxidant enzymes activity of both halophytic grasses under drought stress.

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Proline and Antioxidant Enzyme Activities in Some Strawberry Cultivars under Drought and Recovery

Notulae Botanicae Horti Agrobotanici Cluj-Napoca ◽

10.15835/nbha46211077 ◽

2018 ◽

Vol 46 (2) ◽

pp. 570-578 ◽

Cited By ~ 3

Author(s):

Hatice GULEN ◽

Muge KESICI ◽

Cem CETINKAYA ◽

Sergul ERGIN

Keyword(s):

Polyacrylamide Gel Electrophoresis ◽

Recovery Period ◽

Antioxidant Enzyme Activities ◽

Membrane Injury ◽

Relative Water ◽

Native Polyacrylamide Gel Electrophoresis ◽

Leaf Tissues ◽

Leaf Relative Water Content ◽

Heat Tolerant ◽

Effects Of Drought

This study examines the molecular effects of drought and recovery applications on four strawberry (Fragaria × ananassa Duch) cultivars known as relatively heat-tolerant [‘Redlands Hope’ (‘R. Hope’) and ‘Camarosa’] and heat-sensitive [‘Festival’ and ‘Cal. Giant-3’ (‘CG-3’)]. After frigo seedlings were grown in a greenhouse for eight weeks (until they had five-six leaves) in a pot, 10% Poliethyleneglycol 6000 (PEG) was applied in order to compose drought condition for 15 days. Following PEG application, plants were watered without PEG for 15 days to observe the recovery. Leaf relative water content (RWC), loss of turgidity, cell membrane injury, proline content and the activities of ascorbate peroxidase (APX, EC 1.1.11.1), glutathione reductase (GR, EC 1.6.4.2) and peroxidase (PRX, EC 1.11.1.7) were determined in leaf tissues taken from plants at the end of the drought and recovery applications. Although membrane injury was increased by the PEG application, it reduced almost to the control levels at the end of the recovery period. Proline contents were found higher in cvs. ‘R. Hope’ and ‘Camarosa’ than cvs. ‘Festival’ and ‘CG-3’ under drought conditions. APX and GR enzymes showed great activities in drought applied plants of heat-tolerant cultivars. Native polyacrylamide gel electrophoresis (PAGE) of basic PRX isozymes yielded three protein bands with Rf = 0.45, Rf = 0.55 and Rf = 0.60 that were sharply increased their activities by PEG and recovery applications in cvs. ‘R. Hope’ and ‘Camarosa’. In addition, leaves of all cultivars exposed to drought rolled up and had brown colour in various level as an indication of tolerance. Indeed ‘Camarosa’ and ‘R. Hope’ showed better recovery performance after rewatering than ‘Festival’ and ‘CG-3’. All data indicated that heat-tolerant cultivars showed relatively drought tolerance in this study.

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CORRELATION AND HERITABILITY ESTIMATION OF VARIOUS SEEDLING TRAITS IN BRASSICA NAPUS L. UNDER WATER DEFICIT CONDITIONS

Journal of Plant Stress Physiology ◽

10.25081/jpsp.2017.v3.3385 ◽

2017 ◽

pp. 26-30

Author(s):

Asif Ali, Muhammad Zafar Iqbal, Muhammad Hayder Bin Khalid, Nazir Ahmad ◽

Ameer Bibi

Keyword(s):

Leaf Area ◽

Water Deficit ◽

Shoot Length ◽

Water Deficit Stress ◽

Brassica Napus L ◽

Marginal Lands ◽

Chlorophyll Contents ◽

Seedling Traits ◽

Relative Water ◽

Shoot Weight

Brassica napus L. contains edible oil ensuring safe limits for human health. But farmers prefer to use their fertile land for main crops and only marginal lands for oilseed. Those marginal lands usually face water scarcity and other a-biotic stresses that affect the normal growth and development of plant. Here we tested three levels of water deficit stress (control, medium and high) under controlled conditions. Seedlings were analyzed for various seedling traits to measure the relative effect of different levels water deficit stress. Graphical trends depicts that increasing level of water deficit stress causes declines in leaf area, root length, shoot length, fresh and dry weight of roots and shoots. Correlation coefficients exposed positive significant correlation of fresh shoot weight with chlorophyll contents, relative water contents, leaf area and shoot length at both genotypic and phenotypic level. Path coefficient analysis displayed high direct effects on fresh shoot weight were due to relative water content, root to shoot ratio. Chlorophyll contents, relative water content, leaf area, root length and shoot length showed high broad sense heritability (h2BS) coupled with high genetic advance (GA). These traits could be focused while breeding for water deficit conditions.

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Consequences of the water deficit on water relations and symbiosis in Vigna unguiculata cultivars

Plant Soil and Environment ◽

10.17221/1615-pse ◽

2009 ◽

Vol 55 (No. 4) ◽

pp. 139-145 ◽

Cited By ~ 1

Author(s):

A.K.S. Lobato ◽

R.C.L. Costa ◽

C.F. Oliveira Neto ◽

B.G. Santos Filho ◽

M.C. Gonçalves-Vidigal ◽

...

Keyword(s):

Stomatal Conductance ◽

Nitrate Reductase ◽

Water Deficit ◽

Water Relations ◽

Vigna Unguiculata ◽

Dry Matter ◽

Reductase Activity ◽

Water Deficiency ◽

Relative Water ◽

Leaf Relative Water Content

The study aimed at evaluating and comparing changes provoked by the water deficit on water relations and nitrogen fixation in two Vigna unguiculata cultivars, as well as at indicating which cultivar is more tolerant under water deficiency. The experimental design used was entirely randomized in factorial scheme, with 2 cultivars (Pitiuba and Pérola) and 2 water regimes (control and stress). The parameters evaluated were the leaf relative water content, stomatal conductance, transpiration rate, nodule number, nodule dry matter, nitrate reductase enzyme activity, ureide concentration and leghemoglobin in nodule. The stomatal conductance of the Pitiuba and Pérola cultivars under water deficit were 0.20 and 0.01 mmol H2O/m2/s, respectively. The nitrate reductase activity of the plants under stress was significantly reduced in both cultivars. The leghemoglobin in the Pitiuba and Pérola cultivars under water stress had the concentrations of 58 and 41 g/kg dry matter, respectively. The parameters investigated in this study suggest that the Pitiuba cultivar under water deficit suffers from smaller changes, when compared with Pérola cultivar.

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Photosynthetic Performance of Potato (Solanum tuberosum) Subject to Water Deficit Regulated with Mulched Drip Irrigation

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.405-408.2130 ◽

2013 ◽

Vol 405-408 ◽

pp. 2130-2133 ◽

Cited By ~ 2

Author(s):

Heng Jia Zhang ◽

Jing Li

Keyword(s):

Leaf Area ◽

Water Deficit ◽

Drip Irrigation ◽

Dry Matter ◽

Growth Stages ◽

Soil Water Deficit ◽

Area Index ◽

Relative Growth ◽

Leaf Area Duration ◽

Mulched Drip Irrigation

An experiment was carried out to determine the effect of mulched drip irrigation under water deficit on leaf area index (LAI), leaf area duration (LAD), dry matter (DM) and relative growth rate (RGR) of potato in an arid environment. Five water deficit treatments and a full irrigation control were established to subject potato to various levels of soil water deficit at different crop growth stages. The result indicated that potato LAI and LAD at all the determined growth stages were not reduced under water deficit regulation. Additionally, the DM and RGR at starch accumulation were not significantly decreased under water deficit either. Therefore, medium soil water deficit regulated at 55%~65% of field capacity with mulched drip irrigation at potato tuber initiation could be used to effectively improve leaf area index, leaf area duration, dry matter and relative growth rate of plants.

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Growth improvement of Lolium multiflorum Lam. induced by seed inoculation with fungus suspension of Xerocomus badius and Serendipita indica

AMB Express ◽

10.1186/s13568-019-0865-7 ◽

2019 ◽

Vol 9 (1) ◽

Cited By ~ 2

Author(s):

Binghua Liu ◽

Xinghong Liu ◽

Fangchun Liu ◽

Hailin Ma ◽

Bingyao Ma ◽

...

Keyword(s):

Seed Germination ◽

Growth Promotion ◽

Growth Parameters ◽

Lolium Multiflorum ◽

Biomass Accumulation ◽

Basal Diameter ◽

Beneficial Effects ◽

Mutualistic Symbiosis ◽

Relative Water ◽

Leaf Relative Water Content

Abstract In this study, a pot experiment was carried out in greenhouse to investigate the potentials of Xerocomus badius and Serendipita indica to penetrate and colonize roots of ryegrass (Lolium multiflorum Lam.) and to induce beneficial effects on seed germination and seedling growth. The results showed that X. badius and S. indica successfully colonized in the root system of L. multiflorum seedlings and the root colonization rate was 72.65% and 88.42%, respectively. By microscopy, the hyphae, chlamydospores and spores produced by S. indica were observed in roots cortex of L. multiflorum seedlings. In comparison with the non-inoculated seedlings, seedlings inoculated with X. badius and S. indica showed significant increase in growth parameters with plant height, basal diameter, biomass accumulation, relative growth rate, leaf relative water content and chlorophyll content. Also, we found that seedlings inoculated with S. indica exhibited a greater growth-promotion as compared with X. badius-inoculated seedlings. No significant influence of the two fungus application has been observed with respect to seed germination. It suggested that well establishments of mutualistic symbiosis between L. multiflorum and X. badius or S. indica were not so essential to seed germination but contributed highly to the survival and growth of the seedlings.

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Correlations of Leaf Relative Water Content, Canopy Temperature, and Spectral Reflectance in Perennial Ryegrass Under Water Deficit Conditions

HortScience ◽

10.21273/hortsci.44.2.459 ◽

2009 ◽

Vol 44 (2) ◽

pp. 459-462 ◽

Cited By ~ 19

Author(s):

Yiwei Jiang ◽

Huifen Liu ◽

Van Cline

Keyword(s):

Soil Moisture ◽

Water Content ◽

Water Deficit ◽

Perennial Ryegrass ◽

Relative Water Content ◽

Water Status ◽

Canopy Temperature ◽

Leaf Water ◽

Relative Water ◽

Leaf Relative Water Content

Accurate, rapid, and nondestructive estimates of turfgrass leaf water status are important for site-specific irrigation and drought stress management. The objective of this study was to identify changes and correlations among the canopy reflectance, canopy temperature, and leaf relative water content (RWC) of perennial ryegrass (Lolium perenne L.) under water deficit conditions. Six cultivars of perennial ryegrass were subjected to dry-downs in the field from May to Aug. 2007 and from June to Aug. 2008. Turf quality was positively correlated with soil moisture (SM), RWC, and normalized difference vegetation index (NDVI), but negatively correlated with canopy and ambient temperature differentials (ΔT). ΔT was well correlated with RWC (r = –0.77 to –0.78) and SM (r = –0.66 to –0.74), whereas SM was correlated with RWC (r = 0.64 to 0.74) across seasons in both years. When a wide range of stress symptoms occurred in July and Aug., RWCs became highly correlated with ΔT (r = –0.80 to –0.89) and NDVI (r = 0.77 to 0.81), whereas ΔT was correlated with NDVI (r = –0.70 to –0.80) in both years. SM was well correlated with RWC (r = 0.71 to 0.80), NDVI (r = 0.70 to 0.73), and ΔT (r = –0.76 to –0.78) in July and August in both years. These results suggest that changes in ΔT can be used to predict well the leaf water and soil moisture content of perennial ryegrass under water deficit conditions. Combined with NDVI, the correlations can be used for direct mapping of the variability in grass water status, thus improving irrigation management.

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Short communication: Physiological response to drought in North Sulawesi (Indonesia) local rice (Oryza sativa) cultivars at the tissue level in hydroponic culture

Biodiversitas Journal of Biological Diversity ◽

10.13057/biodiv/d220108 ◽

2020 ◽

Vol 22 (1) ◽

Author(s):

Song Ai Nio ◽

RISA JUNITA MEREH ◽

DANIEL PETER MANTILEN LUDONG

Keyword(s):

Water Content ◽

Water Deficit ◽

Relative Water Content ◽

Physiological Response ◽

Tissue Level ◽

Hydroponic Culture ◽

North Sulawesi ◽

Relative Water ◽

Leaf Relative Water Content ◽

Peg 8000

Abstract. Nio SA, Mereh RJ, Ludong DPM. 2021. Short communication: Physiological response to drought in North Sulawesi (Indonesia) local rice (Oryza sativa) cultivars at the tissue level in hydroponic culture. Biodiversitas 22: 58-64. Water availability influenced the metabolism processes in the plants and this condition could change water balance in the cells as well as restricting the growth and production of crops. This study aimed to evaluate the effect of in vitro polyethylene glycol (PEG) 8000-induced water deficit on the relative water content and chlorophylls (total, a, and b) concentration as physiological response in leaf segments of North Sulawesi (Indonesia) local rice cultivars (cvs. Superwin, Ombong, Burungan, and Temo). The rice leaves were cut into 1 cm x 1 cm segments and were treated under both control (0 MPa) and water deficit (-0.25 and -0.5 MPa) using PEG 8000 as osmoticum. The experiment was factorial completely randomized block design with 4 sampling times (0, 4, 8, and 12 hours) and three replicates. Interaction of water deficit duration and PEG 8000 treatment resulted in a significant difference in leaf relative water content. The leaf relative water content at PEG -0.5 MPa after 8 and 12 hours of treatments was lower than at control (PEG 0 MPa) when the treatment commenced (0 hour). The concentration of chlorophylls (total, a, and b) were significant differences among cultivars and water deficit durations. This study showed that leaf relative water content was a potential physiological indicator of PEG 8000-induced water deficit in North Sulawesi local rice at the tissue level in hydroponic culture.

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