Fabrication of BSA Loaded Poly(Caprolactone) (PCL) Microsphere Incorporated Chitosan Scaffolds for Tissues Engineering Application

2014 ◽  
Vol 695 ◽  
pp. 199-202
Author(s):  
Mad Jin Rashid ◽  
Lari Ali Reza ◽  
Naznin Sultana
Keyword(s):  
Extracellular Matrix ◽  
Engineering Application ◽  
Chitosan Solution ◽  
Living Cells ◽  
Porous Scaffolds ◽  
High Porosity ◽  
Chitosan Scaffolds ◽  
Artificial Extracellular Matrix ◽  
Poly Caprolactone ◽  
Suitable Environment

Scaffolds-based tissues engineering involves the combination of an artificial extracellular matrix (ECM), living cells, with high porosity and well connected pores that will provide suitable environment for cells. In this study, firstly, poly (caprolactone) (PCL)-based microspheres were synthesized and characterized. Bovine serum albumin (BSA) (0.04% w/v) was added into the microspheres produced from 5% (w/v) PCL concentration. BSA loaded microspheres were then incorporated into chitosan solution to fabricate porous scaffolds. The scaffolds were then characterized using different techniques.

scholarly journals Polymeric Scaffolds in Tissue Engineering Application: A Review

2011 ◽  
Vol 2011 ◽  
pp. 1-19 ◽  
Author(s):  
Brahatheeswaran Dhandayuthapani ◽  
Yasuhiko Yoshida ◽  
Toru Maekawa ◽  
D. Sakthi Kumar
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Engineering Application ◽  
Biologically Active ◽  
Porous Scaffolds ◽  
Tissue Engineering Application ◽  
Tissue Engineering Scaffolds ◽  
Matrix Deposition ◽  
Bioactive Agents ◽  
Regenerate Tissue

Current strategies of regenerative medicine are focused on the restoration of pathologically altered tissue architectures by transplantation of cells in combination with supportive scaffolds and biomolecules. In recent years, considerable interest has been given to biologically active scaffolds which are based on similar analogs of the extracellular matrix that have induced synthesis of tissues and organs. To restore function or regenerate tissue, a scaffold is necessary that will act as a temporary matrix for cell proliferation and extracellular matrix deposition, with subsequent ingrowth until the tissues are totally restored or regenerated. Scaffolds have been used for tissue engineering such as bone, cartilage, ligament, skin, vascular tissues, neural tissues, and skeletal muscle and as vehicle for the controlled delivery of drugs, proteins, and DNA. Various technologies come together to construct porous scaffolds to regenerate the tissues/organs and also for controlled and targeted release of bioactive agents in tissue engineering applications. In this paper, an overview of the different types of scaffolds with their material properties is discussed. The fabrication technologies for tissue engineering scaffolds, including the basic and conventional techniques to the more recent ones, are tabulated.

scholarly journals Electrospun Nanofibers and their Application in Tissue Repair and Engineering

2020 ◽  
Author(s):  
Sareh Arjmand ◽  
Alireza Partovi Baghdadeh ◽  
Amin Hamidi ◽  
Seyed Omid Ranaei Siadat
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
High Surface ◽  
Engineering Application ◽  
Volume Ratio ◽  
Biologically Active ◽  
Dimensional Structure ◽  
Porous Scaffolds ◽  
Tissue Engineering Application ◽  
Effective Parameters

Introduction: Tissue engineering is the repair and replacement of damaged tissues and requires a combination of cells, growth factor and porous scaffolds. Scaffolds, as one of the main components in tissue engineering, are used as a template for tissue regeneration and induction and guidance of growth of the new and biologically active tissues. An ideal scaffold in tissue engineering, imitating an extracellular matrix, provides a suitable environment for adhesion, growth and cell proliferation. Scaffolds have also been used as the carriers for the controlled delivery of drugs and proteins. Variety of porous scaffolds, fabricated from biological and synthetic materials and using different manufacturing methods, have been introduced. Among them nanofibrous scaffolds have attracted great attention due to remarkable advantages including the highly porous three-dimensional structure with interconnected cavities which enable the transportation of food and waste materials, as well as high surface to volume ratio. So far, different methods and techniques have been introduced for production of scaffolds with structures similar to the extracellular matrix. Amongst them electrospinning, due to easiness and more control over effective parameters, are preferred. The present study make a review about the used materials and various methods of nanofibrous scaffold fabrication using electrospinning technology, with emphasis on the use of tissue engineering application. It also discussed about the progress and challenges ahead and the goals and perspective presented for this approach.

Recent Advances in Extracellular Matrix for Engineering Stem Cell Responses

2019 ◽  
Vol 26 (34) ◽  
pp. 6321-6338 ◽  
Author(s):  
Shuaimeng Guan ◽  
Kun Zhang ◽  
Jingan Li
Keyword(s):  
Stem Cells ◽  
Extracellular Matrix ◽  
Stem Cell ◽  
Cell Attachment ◽  
Biological Significance ◽  
Engineering Application ◽  
Advanced Medical Technology ◽  
Challenges And Opportunities ◽  
Physical Functions ◽  
Differential Ability

Stem cell transplantation is an advanced medical technology, which brings hope for the treatment of some difficult diseases in the clinic. Attributed to its self-renewal and differential ability, stem cell research has been pushed to the forefront of regenerative medicine and has become a hot topic in tissue engineering. The surrounding extracellular matrix has physical functions and important biological significance in regulating the life activities of cells, which may play crucial roles for in situ inducing specific differentiation of stem cells. In this review, we discuss the stem cells and their engineering application, and highlight the control of the fate of stem cells, we offer our perspectives on the various challenges and opportunities facing the use of the components of extracellular matrix for stem cell attachment, growth, proliferation, migration and differentiation.

scholarly journals Porous scaffolds with the structure of an interpenetrating polymer network made by gelatin methacrylated nanoparticle-stabilized high internal phase emulsion polymerization targeted for tissue engineering

RSC Advances ◽  
2021 ◽  
Vol 11 (37) ◽  
pp. 22544-22555
Author(s):  
Atefeh Safaei-Yaraziz ◽  
Shiva Akbari-Birgani ◽  
Nasser Nikfarjam
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Cell Growth ◽  
Polymer Network ◽  
Synthetic Polymers ◽  
Tissue Scaffolds ◽  
Interpenetrating Polymer Network ◽  
Porous Scaffolds ◽  
Promising Strategy ◽  
Internal Phase

The interlacing of biopolymers and synthetic polymers is a promising strategy to fabricate hydrogel-based tissue scaffolds to biomimic a natural extracellular matrix for cell growth.

scholarly journals Electrospun Nanofibers of Natural and Synthetic Polymers as Artificial Extracellular Matrix for Tissue Engineering

Nanomaterials ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 21
Author(s):  
Mina Keshvardoostchokami ◽  
Sara Seidelin Majidi ◽  
Peipei Huo ◽  
Rajan Ramachandran ◽  
Menglin Chen ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Polymer Composite ◽  
Electrospun Nanofibers ◽  
Synthetic Polymer ◽  
Inorganic Materials ◽  
Host Cells ◽  
Natural Polymer ◽  
Reinforced Polymers ◽  
Nanofibrous Scaffolds ◽  
Artificial Extracellular Matrix

Many types of polymer nanofibers have been introduced as artificial extracellular matrices. Their controllable properties, such as wettability, surface charge, transparency, elasticity, porosity and surface to volume proportion, have attracted much attention. Moreover, functionalizing polymers with other bioactive components could enable the engineering of microenvironments to host cells for regenerative medical applications. In the current brief review, we focus on the most recently cited electrospun nanofibrous polymeric scaffolds and divide them into five main categories: natural polymer-natural polymer composite, natural polymer-synthetic polymer composite, synthetic polymer-synthetic polymer composite, crosslinked polymers and reinforced polymers with inorganic materials. Then, we focus on their physiochemical, biological and mechanical features and discussed the capability and efficiency of the nanofibrous scaffolds to function as the extracellular matrix to support cellular function.

Promotion of Angiogenesis by an Artificial Extracellular Matrix Protein Containing the Laminin-1-Derived IKVAV Sequence

2009 ◽  
Vol 20 (9) ◽  
pp. 1759-1764 ◽  
Author(s):  
Makiko Nakamura ◽  
Kumiko Yamaguchi ◽  
Masayasu Mie ◽  
Makoto Nakamura ◽  
Keiichi Akita ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Matrix Protein ◽  
Extracellular Matrix Protein ◽  
Artificial Extracellular Matrix ◽  
Laminin 1

Transformable Nanomaterials as an Artificial Extracellular Matrix for Inhibiting Tumor Invasion and Metastasis

ACS Nano ◽  
2017 ◽  
Vol 11 (4) ◽  
pp. 4086-4096 ◽  
Author(s):  
Xiao-Xue Hu ◽  
Ping-Ping He ◽  
Guo-Bin Qi ◽  
Yu-Juan Gao ◽  
Yao-Xin Lin ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Tumor Invasion ◽  
Invasion And Metastasis ◽  
Artificial Extracellular Matrix

Construction of Hybrid Cell Spheroids Using Cell-Sized Cross-Linked Nanogel Microspheres as an Artificial Extracellular Matrix

2021 ◽  
Author(s):  
Shunya Hayashi ◽  
Yoshihiro Sasaki ◽  
Hirotaka Kubo ◽  
Shin-ichi Sawada ◽  
Naoya Kinoshita ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Hybrid Cell ◽  
Cell Spheroids ◽  
Artificial Extracellular Matrix

scholarly journals Fabrication and Shape Memory Characteristics of Highly Porous Ti-Nb-Mo Biomaterials

2017 ◽  
Vol 62 (2) ◽  
pp. 1367-1370 ◽  
Author(s):  
Y.-W. Kim ◽  
T.W. Mukarati
Keyword(s):  
Martensitic Transformation ◽  
Shape Memory ◽  
Shape Memory Effect ◽  
Memory Effect ◽  
Differential Scanning Calorimetric ◽  
Porous Scaffolds ◽  
High Porosity ◽  
Porous Ti ◽  
Human Body Temperature ◽  
Rapidly Solidified

AbstractNon-toxic Ti-Nb-Mo scaffolds were fabricated by sintering rapidly solidified alloy fibers for biomedical applications. Microstructure and martensitic transformation behaviors of the porous scaffolds were investigated by means of differential scanning calorimetric and X-ray diffraction. Theα″–βtransformation occurs in the as-solidified fiber and the sintered scaffolds. According to the compressive test of the sintered scaffolds with 75% porosity, they exhibit good superelasticity and strain recovery ascribed to the stress-induced martensitic transformation and the shape memory effect. Because of the high porosity of the scaffolds, an elastic modulus of 1.4 GPa, which matches well with that of cancellous bone, could be obtained. The austenite transformation finishing temperature of 77Ti-18Nb-5Mo alloy scaffolds is 5.1°C which is well below the human body temperature, and then all mechanical properties and shape memory effect of the porous 77Ti-18Nb-5Mo scaffolds are applicable for bon replacement implants.

scholarly journals Porcine Breast Extracellular Matrix Hydrogel for Spatial Tissue Culture

2018 ◽  
Vol 19 (10) ◽  
pp. 2912 ◽  
Author(s):  
Girdhari Rijal ◽  
Jing Wang ◽  
Ilhan Yu ◽  
David Gang ◽  
Roland Chen ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Human Mammary Epithelial Cell ◽  
Tumor Formation ◽  
Receptor Expression ◽  
Cell Surface Receptor ◽  
Breast Diseases ◽  
Porous Scaffolds ◽  
Ecm Proteins ◽  
Surface Receptor

Porcine mammary fatty tissues represent an abundant source of natural biomaterial for generation of breast-specific extracellular matrix (ECM). Here we report the extraction of total ECM proteins from pig breast fatty tissues, the fabrication of hydrogel and porous scaffolds from the extracted ECM proteins, the structural properties of the scaffolds (tissue matrix scaffold, TMS), and the applications of the hydrogel in human mammary epithelial cell spatial cultures for cell surface receptor expression, metabolomics characterization, acini formation, proliferation, migration between different scaffolding compartments, and in vivo tumor formation. This model system provides an additional option for studying human breast diseases such as breast cancer.

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