Detection of Silver Nanoparticles Internalization into Petunia, Petunia hybrida, Isolated Protoplasts

2012 ◽  
Vol 622-623 ◽  
pp. 878-882
Author(s):  
Roohangiz Naderi ◽  
Sarah Jafari Rad ◽  
Aliakbar Saboor Yaraghi ◽  
Mehdi Farhoodi ◽  
Mina Nemati
Keyword(s):  
Electron Microscopy ◽  
Drug Delivery ◽  
Silver Nanoparticles ◽  
Cell Membrane ◽  
Clinical Diagnosis ◽  
Petunia Hybrida ◽  
Nano Particles ◽  
Isolated Protoplasts ◽  
Long Period ◽  
Petunia Protoplasts

The use of nanoparticles has spannded many divisions of biological fields including sensors, drug delivery and clinical diagnosis. These functions all based on the most important action in cells called Endocytosis. The study of endocytosis in higher order plants remained inactive for a long period of time. In this work we use Silver Nano Particles (S.N.Ps) as a endocytosic substances were incubated by isolated protoplast of petunia .Concurrently, with the study of internalization, their appear aggregation inter and intracellular were investigated by Transmissions Electron Microscopy (TEM). Results and observations showed that the S.N.Ps crossed the cell membrane and internalized in to the cells. It can be assumed that the particles with the organic functionality – PEG coated – were internalized by the petunia protoplasts.

scholarly journals Biogenic synthesis of silver nanoparticles using Paenibacillus sp. in-vitro and their antibacterial, anticancer activity assessment against human colon tumour cell line

2021 ◽  
Vol 42 (1) ◽  
pp. 118-127
Author(s):  
N. Sreenivasa ◽  
◽  
B.P. Meghashyama ◽  
S.S. Pallavi ◽  
C. Bidhayak ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Cell Membrane ◽  
Cancer Cells ◽  
Human Colon ◽  
Tumour Cells ◽  
X Ray ◽  
Colon Tumour ◽  
Paenibacillus Sp

Aim: To evaluate the antibacterial and anticancer activities of silver nanoparticles (AgNPs) synthesized from aqueous extract of Paenibacillus sp. strain NS-36. Methodology: The green synthesized AgNPs were characterized by UV-Vis. spectroscopy, Fourier Transform Infrared Spectroscopy, Atomic Force Microscopy, X-Ray Diffraction, Scanning Electron Microscopy, Energy Dispersive X-ray Spectroscopy, and Transmission Electron Microscopy. Antibacterial activity was assessed against pathogenic bacteria by using agar well diffusion method and the anticancer activity was evaluated against Human Colon Tumour-116 cell line using 96 well plate cell proliferation assay. Results: The synthesized AgNPs showed UV-Vis absorbance peak at 416 nm. The characterization analyses revealed the shape as spherical and size ranging from 17.49 to 52.85 nm and the presence of different functional groups and elements that involved in the stabilization and capping. The antibacterial activity revealed that AgNPs have good inhibitory action on pathogens, whereas the results of anticancerous assessment indicated that AgNPs have a dose-dependent activity on the Human Colon Tumour-116 cancer cells and the IC50 value was found to be 81.45 μg ml-1. Interpretation: The AgNPs exhibited considerable antibacterial activity against bacterial pathogens by rupturing and damaging the cell membrane. The AgNPs release silver ions into the cell once they attached to the cell membrane and disrupt the bacterial DNA replication. The AgNPs are toxic to tumour cells and induce intracellular reactive oxygen species which damage cells. Along with ROS, the rounding and shrinkage of tumour cells caused by AgNPs reduced the % viability of cancer cells. Key words: Antimicrobial activity, Cytotoxicity, Paenibacillus sp, Silver nanoparticles, Tumour cells

scholarly journals Biogenic Synthesis of Silver and Gold Nano Particles using Fungal Species

2018 ◽  
Vol 1 (01) ◽  
pp. 25-30
Author(s):  
S M Shaikh ◽  
T J Shaikh
Keyword(s):  
Drug Delivery ◽  
Silver Nanoparticles ◽  
Aspergillus Niger ◽  
Fungal Species ◽  
Fungal Strain ◽  
Nano Particles ◽  
Biogenic Synthesis ◽  
Gold And Silver Nanoparticles ◽  
Low Toxicity ◽  
Gold Nano Particles

Gold and Silver nanoparticles synthesized by various technique have received special attention because they have found potential application in many fields such as catalysis, sensors, drug delivery system. Additionally, silver nanaparticles possess an excellent biocompatibility and low toxicity. The present investigation have done to check out the synthetic compatibility of  Nano particles of some fungal strain i.e. Alternaria alternata, Aspergillus niger, Fusarium oxysporum, Pennicelium digitatum and Pennicelium spp.

scholarly journals Preparation method of Silver Nano particles

2020 ◽  
Vol 3 (2) ◽  
pp. 1-8
Author(s):  
Muhanned A E Al-Saedy
Keyword(s):  
Drug Delivery ◽  
Silver Nanoparticles ◽  
Pharmaceutical Industry ◽  
Water Purification ◽  
Preparation Method ◽  
Human Life ◽  
Biological Properties ◽  
Nano Particles ◽  
Ag Nps ◽  
Utility Evaluation

The last few years have perceived the most important development in the field of metals nanoparticle. As they have emerged a highly demanding chemical, physical and biological properties, thereby they employed in the various chemical and pharmaceutical industry. Silver as a metal with exceptional properties, its nanoparticles have devoted significant efforts for their synthesis and utility evaluation. In this review, the most recent and important approaches for the synthesis of silver nanoparticles will be covered along with the use of Ag NPs in a various disciplines especially those that are related to human life such as medicine, drug delivery and water purification. Besides reviewing several different opinions about the risks resulting from the synthesis and use of this type of materials

scholarly journals Decoration of Silver Nanoparticles on Multiwalled Carbon Nanotubes: Antibacterial Mechanism and Ultrastructural Analysis

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Ngo Xuan Dinh ◽  
Nguyen Van Quy ◽  
Tran Quang Huy ◽  
Anh-Tuan Le
Keyword(s):  
Electron Microscopy ◽  
Carbon Nanotubes ◽  
Silver Nanoparticles ◽  
Cell Membrane ◽  
Multiwalled Carbon Nanotubes ◽  
Ultrastructural Changes ◽  
Physical Interaction ◽  
Ag Nps ◽  
Multiwalled Carbon ◽  
Membrane Function

Recently, development of carbon nanocomposites composed of carbon nanostructures and metal nanoparticles has attracted much interests because of their large potential for technological applications such as catalyst, sensor, biomedicine, and disinfection. In this work, we established a simple chemistry method to synthesize multiwalled carbon nanotubes (MWCNTs) decorated with silver nanoparticles (Ag-NPs) using a modified photochemical reaction (Tollens process). The formation and interaction of Ag-NPs with functionalized groups on the surface of MWCNTs were analyzed by X-ray diffraction, transmission electron microscopy, Raman spectroscopy, and Fourier-transform infrared spectroscopy. The average size of Ag-NPs on the MWCNTs was approximately ~7 nm with nearly uniform size distribution. Antibacterial effect of Ag-MWCNTs nanocomposites was evaluated against two pathogenic bacteria including Gram-negativeEscherichia Coliand Gram-positiveStaphylococcus aureusbacteria. Interaction and bactericidal mechanism of Ag-MWCNTs with tested bacteria was studied by adapting the electron microscopy. Analysis on ultrastructural changes of bacterial cells indicates that antibacterial action mechanism of Ag-MWCNTs is physical interaction with cell membrane, the large formation of cell-Ag-MWCNTs aggregates, and faster destructibility of cell membrane and disruption of membrane function, hence resulting in cells death.

scholarly journals Salvadora persica mediated synthesis of silver nanoparticles and their antimicrobial efficacy

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Hammad Arshad ◽  
Muhammad A. Sami ◽  
Saima Sadaf ◽  
Umer Hassan
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Antimicrobial Properties ◽  
Nano Particles ◽  
Antimicrobial Efficacy ◽  
Root Extract ◽  
Bacterial Cells ◽  
Salvadora Persica ◽  
Nitrogenous Compounds ◽  
Vis Spectroscopy

AbstractSilver nanoparticles (AgNPs) exhibit strong antimicrobial properties against many pathogens. Traditionally employed chemical methods for AgNPs synthesis are toxic for the environment. Here, we report a quicker, simpler, and environmentally benign process to synthesize AgNPs by using an aqueous ‘root extract’ of Salvadora persica (Sp) plant as a reducing agent. The synthesized Salvadora persica nano particles (SpNPs) showed significantly higher antimicrobial efficacy compared to earlier reported studies. We characterized SpNPs using UV–Vis spectroscopy, Fourier Transform Infrared Spectroscopy (FTIR), Transmission Electron Microscopy (TEM), Field Emission Scanning Electron Microscopy (FE-SEM), Dynamic Light Scattering (DLS) and X-ray powder diffraction (P-XRD). UV–Vis spectrum showed the highest absorbance at 420 nm. FTIR analysis depicts presence of bond stretching including OH– (3300 cm−1), C=N– (2100 cm−1) and NH– (1630 cm−1) which are attributed in the involvement of phenolics, proteins or nitrogenous compounds in reduction and stabilization of AgNPs. TEM, FE-SEM and DLS analysis revealed the spherical and rod nature of SpNPs and an average size of particles as 37.5 nm. XRD analysis showed the presence of the cubic structure of Ag which confirmed the synthesis of silver nanoparticles. To demonstrate antimicrobial efficacy, we evaluated SpNPs antimicrobial activity against two bacterial pathogens (Escherichia coli (ATCC 11229) and Staphylococcus epidermidis (ATCC 12228)). SpNPs showed a significantly high inhibition for both pathogens and minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were found to be 0.39 µg/mL and 0.78 µg/mL for E. coli while 0.19 µg/mL and 0.39 µg/mL for S. epidermidis respectively. Further, Syto 16 staining of bacterial cells provided a supplemental confirmation of the antimicrobial efficacy as the bacterial cells treated with SpNPs stop to fluoresce compared to the untreated bacterial cells. Our highly potent SpNPs will likely have a great potential for many antimicrobial applications including wound healing, water purification, air filtering and other biomedical applications.

High-voltage electron microscopy of patch-clamped membranes

1987 ◽  
Vol 45 ◽  
pp. 582-583
Author(s):  
F. Sachs ◽  
M. J. Song
Keyword(s):  
Electron Microscopy ◽  
Cell Membrane ◽  
Patch Clamp ◽  
High Voltage Electron Microscopy ◽  
Small Patch ◽  
Cellular Electrophysiology ◽  
Voltage Electron ◽  
Electron Microscopes ◽  
Patch Technique ◽  
Membrane Patch

Cellular electrophysiology has been revolutionized by the introduction of patch clamp techniques. The patch clamp records current from a small patch of the cell membrane which has been sucked into a glass pipette. The membrane patch, a few micons in diameter, is attached to the glass by a seal which is electrically, diffusionally and mechanically tight. Because of the tight electrical seal, the noise level is low enough to record the activity of single ion channels over a time scale extending from 10μs to days. However, although the patch technique is over ten years old, the patch structure is unknown. The patch is inside a glass pipette where it has been impossible to see with standard electron microscopes. We show here that at 1 Mev the glass pipette is transparent and the membrane within can be seen with a resolution of about 30 A.

Study on erythrocytes by SEM photogrammetry (SEMP) technique

1990 ◽  
Vol 48 (3) ◽  
pp. 724-725
Author(s):  
Tong Wensheng ◽  
Lu Lianhuang ◽  
Zhang Zhijun
Keyword(s):  
Quantitative Analysis ◽  
Cell Membrane ◽  
Clinical Diagnosis ◽  
Human Body ◽  
Blood Cells ◽  
Three Dimensional ◽  
Normal Person ◽  
Blood Disease ◽  
Computation Technique ◽  
Important Basis

This is a combined study of two diffirent branches, photogrammetry and morphology of blood cells. The three dimensional quantitative analysis of erythrocytes using SEMP technique, electron computation technique and photogrammetry theory has made it possible to push the study of mophology of blood cells from LM, TEM, SEM to a higher stage, that of SEM P. A new path has been broken for deeply study of morphology of blood cells.In medical view, the abnormality of the quality and quantity of erythrocytes is one of the important changes of blood disease. It shows the abnormal blood—making function of the human body. Therefore, the study of the change of shape on erythrocytes is the indispensable and important basis of reference in the clinical diagnosis and research of blood disease.The erythrocytes of one normal person, three PNH Patients and one AA patient were used in this experiment. This research determines the following items: Height;Length of two axes (long and short), ratio; Crevice in depth and width of cell membrane; Circumference of erythrocytes; Isoline map of erythrocytes; Section map of erythrocytes.

Long-period modulated superstructures in Pd-12.5 at.%Ce and Pd-15 at.%Ce alloys

1990 ◽  
Vol 48 (4) ◽  
pp. 164-165
Author(s):  
Noriyuki Kuwano ◽  
Masaru Itakura ◽  
Kensuke Oki
Keyword(s):  
Electron Microscopy ◽  
Mean Value ◽  
Heavy Elements ◽  
Arc Furnace ◽  
X Ray ◽  
Long Period ◽  
Ultra High Purity ◽  
Heat Treated ◽  
Atomic Scattering ◽  
The Mean

Pd-Ce alloys exhibit various anomalies in physical properties due to mixed valences of Ce, and the anomalies are thought to be strongly related with the crystal structures. Since Pd and Ce are both heavy elements, relative magnitudes of (fcc-fpd) are so small compared with <f> that superlattice reflections, even if any, sometimes cannot be detected in conventional x-ray powder patterns, where fee and fpd are atomic scattering factors of Ce and Pd, and <f> the mean value in the crystal. However, superlattices in Pd-Ce alloys can be analyzed by electron microscopy, thanks to the high detectability of electron diffraction. In this work, we investigated modulated superstructures in alloys with 12.5 and 15.0 at.%Ce.Ingots of Pd-Ce alloys were prepared in an arc furnace under atmosphere of ultra high purity argon. The disc specimens cut out from the ingots were heat-treated in vacuum and electrothinned to electron transparency by a jet method.

Crystal Structures of Fragments D and Double-D from Fibrinogen and Fibrin

1999 ◽  
Vol 82 (08) ◽  
pp. 271-276 ◽  
Author(s):  
Glen Spraggon ◽  
Stephen Everse ◽  
Russell Doolittle
Keyword(s):  
Electron Microscopy ◽  
High Resolution ◽  
Crystal Structures ◽  
Structure And Function ◽  
X Ray ◽  
Long Period ◽  
And Function

IntroductionAfter a long period of anticipation,1 the last two years have witnessed the first high-resolution x-ray structures of fragments from fibrinogen and fibrin.2-7 The results confirmed many aspects of fibrinogen structure and function that had previously been inferred from electron microscopy and biochemistry and revealed some unexpected features. Several matters have remained stubbornly unsettled, however, and much more work remains to be done. Here, we review several of the most significant findings that have accompanied the new x-ray structures and discuss some of the problems of the fibrinogen-fibrin conversion that remain unresolved. * Abbreviations: GPR—Gly-Pro-Arg-derivatives; GPRPam—Gly-Pro-Arg-Pro-amide; GHRPam—Gly-His-Arg-Pro-amide

scholarly journals Effect of calcination temperature on the properties and applications of bio extract mediated titania nano particles

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
N. Saikumari ◽  
S. Monish Dev ◽  
S. Avinaash Dev
Keyword(s):  
Electron Microscopy ◽  
Calcination Temperature ◽  
Surface Properties ◽  
Economic Feasibility ◽  
Nano Particles ◽  
Textile Dye ◽  
Gravimetric Analysis ◽  
Anatase Titania ◽  
Nano Catalyst ◽  
Bet Analysis

AbstractIn order to deal with the arising environmental issues across the globe at present nano particles with unique properties laid a benchmark in the name of nano catalysis. In this work the significance of calcination temperature on the thermal, electronic, structural and surface properties of a nano catalyst produced by sol–gel method using ultrasonic radiation against the disposal of toxic textile pollutants is studied in detail. The extract of tea leaves has been used as a bio-template during the synthesis to revise the crystallite size, surface area, optical absorption potential, and rate of agglomeration of nano sized grains by regulating their physico-chemical and surface properties. The influence of calcination in the transformation of single phased anatase titania to mixed phase anatase–rutile titania and the corresponding outcome in its photocatalytic activity employed in water treatment applications have been verified. The nano catalyst obtained is characterized by X-ray diffraction (XRD), Scanning electron microscopy (SEM), Transition electron microscopy (TEM), Fourier transform infrared spectroscopy (FT-IR), Thermo gravimetric analysis (TGA), Brunaueur Emmett Teller (BET) analysis, UV–Vis diffused reflectance spectroscopy (DRS-UV–Vis) etc. The mesoporosity of the particle was examined using Barrett Joyner Halenda (BJH) model. The enhanced photo catalytic efficiency (about 97.7%) of templated nano titania due to calcination is verified against Congo red, a textile dye under optimized conditions. The nano catalyst produced can be easily separated, recycled to support its economic feasibility.

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