Essential Oils Encapsulation into Nanoliposome and Novel Application of Nanoliposome as a Storage Pesticide

2020 ◽  
Vol 62 ◽  
pp. 96-107
Author(s):  
Zahra Faraji ◽  
Jahanshir Shakarami ◽  
Jaleh Varshosaz ◽  
Shahriar Jafari
Keyword(s):  
Thin Film ◽  
Essential Oil ◽  
Essential Oils ◽  
Storage Stability ◽  
Physicochemical Characteristics ◽  
Release Profile ◽  
Mentha Pulegium ◽  
Preparation Methods ◽  
Mean Size ◽  
Fumigation Toxicity

This study attempted to evaluate the physicochemical characteristics of essential oil nanoliposomes from three plants by two preparation methods including thin film sonication and heating. Also, the durability of fumigation toxicity of the essential oil nanoliposome and pure essential oil of Mentha pulegium against Tribolium castaneum Herbst adults were compared. The smallest mean size of the nanoliposome was prepared by the thin film sonication method (P<0.05). The encapsulation efficiency of the nanoliposome was higher than 96%. Nanoliposmes prepared by using two methods showed high stability during storage. The release profile of essential oils at the heating method showed that the essential oil diffusion from the nanoliposome was in the following order: Mentha pulegium > Ferula gummosa > Zattaria multiflora. These findings indicate that both preparation methods had the same effect on the formation of nanoliposomes, their characteristics, and their storage stability. The durability of fumigation toxicity of the nanoliposome M. pulegium essential oil had a significant effect on mortality of T. castaneum adults in comparison with pure essential oil of this plant. The result showed that nanoliposome encapsulation of M. pulegium essential oil significantly increased fumigation toxicity against T. castaneum. Therefore, essential oil encapsulation into a nanoliposome carrier can potentially be used in pest management.

scholarly journals FUMIGATION TOXICITY OF ESSENTIAL OILS AGAINST Rhyzopertha dominica (F.) IN STORED MAIZE GRAIN

2016 ◽  
Vol 29 (2) ◽  
pp. 435-440 ◽  
Author(s):  
VALDEANY NÚBIA DE SOUZA ◽  
CARLOS ROMERO FERREIRA DE OLIVEIRA ◽  
CLÁUDIA HELENA CYSNEIROS MATOS ◽  
DAIANY KEILA FLORENTINO DE ALMEIDA
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Rhyzopertha Dominica ◽  
Fumigant Toxicity ◽  
Citrus Aurantium ◽  
Mentha Spicata ◽  
Insect Mortality ◽  
Maize Grain ◽  
Potential Use ◽  
Fumigation Toxicity

ABSTRACT: The Rhyzopertha dominica F. (Coleoptera: Bostrichidae) is a primary pest of stored grains in many regions of the world. In this work we evaluated the fumigant activity of essential oils of Ocimum basilicum L., Citrus aurantium L., Mentha spicata L. and Croton pulegiodorus Baill on adult R. dominica in stored maize. Tests were conducted to determine lethals concentrations (CL50 and CL100) and mortality (fumigation).The fumigation test was done in containers made of glass containing 10 individuals of R. dominica, where essential oils were applied at different concentrations: O. basilicum and M. spicata (5, 10, 15, 20, 30 and 40µL/L of air), C. aurantium (10, 20, 30, 40, 50 and 60µL/L of air) and C. pulegiodorus (0, 20, 30, 50, 70 and 90µL/L of air). After 48 hours of exposure to the oils the percentage of insect mortality was evaluated. According to LC50 and LC100 the toxicity of essential oils decreased in the following order: O. basilicum> M. spicata> C. pulegiodorus> C. aurantium. The essential oil of O. basilicum exhibited strong fumigant toxicity against R. dominica adults, with a LC50 value of 17.67 µL/L air and LC100 value of 27.15 µL/L air. The C. aurantium essential oil required higher concentrations than O. basilicum, M. spicata and C. pulegiodorus to kill insects. However, all oils evaluated presented fumigating property to promote the control of R. dominica and demonstrated potential use in the management of this coleoptera.

scholarly journals Effects of cold-storage facility characteristics on the virulence and sporulation of Penicillium expansum and the efficacy of essential oils against blue mold rot of apples

2019 ◽  
Vol 31 (2) ◽  
pp. 301-317
Author(s):  
Bochra A. Bahri ◽  
Ghaya Mechichi ◽  
Wafa Rouissi ◽  
Imtinen Ben Haj Jilani ◽  
Zeineb Ghrabi-Gammar
Keyword(s):  
Population Structure ◽  
Essential Oil ◽  
Essential Oils ◽  
Mycelial Growth ◽  
Spore Production ◽  
Penicillium Expansum ◽  
Storage Facility ◽  
Blue Mold ◽  
Mentha Pulegium ◽  
Fruit Species

AbstractBlue mold rot, caused by Penicillium expansum, is one of the most economically important post-harvest diseases of apple worldwide. The goals of this study were threefold: to evaluate the diversity of P. expansum isolates for mycelial growth, spore production and lesion diameter on apples; to estimate the effects of cold-storage facility conditions on P. expansum population structure; and to investigate the efficacy of three essential oils against P. expansum. The results showed that storage facilities applying fungicides and storing diverse fruit species selected for P. expansum isolates with a larger lesion diameter on apples. In addition, application of fungicides and diversification in stored fruit species significantly select for P. expansum isolates with higher levels of mycelial growth and spore production, respectively. Moreover, the diversity of host species of stored fruit accounted for 38% of the variability observed between storage facilities for the measured fitness parameters in P. expansum isolates and had a stronger effect on P. expansum population structure than fungicide treatment. Essential oils from Mentha pulegium and Syzygium aromaticum significantly decreased mycelial growth and spore production of P. expansum isolates in vitro. Mentha pulegium essential oil also significantly decreased the size of lesions associated with the blue mold rot of apples. Reducing the diversity of stored host species and applying M. pulegium essential oil may be useful in counter-selecting for aggressive P. expansum isolates and reducing losses due to blue mold rot during fruit storage.

scholarly journals Activité Acaricide Des Huiles Essentielles Du Mentha Pulegium, Origanum Compactum Et Thymus Capitatus Sur L’acarien Phytophage Tetranychus Urticae Koch (Acari : Tetranychidae)

2018 ◽  
Vol 14 (3) ◽  
pp. 118 ◽  
Author(s):  
Asmae Bakkali Aissaoui ◽  
Amal El Amrani ◽  
Said Zantar ◽  
Larbi Toukour
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Tetranychus Urticae ◽  
Contact Toxicity ◽  
Mentha Pulegium ◽  
Plant Essential Oils ◽  
Thymus Capitatus ◽  
Origanum Compactum ◽  
Main Component ◽  
Tetranychus Urticae Koch

The use of botanical acaricides extracted from plants as an alternative to replace the chemical acaricides is an interesting and efficient option to control pests and ameliorate their toxic effects to humans and the environment. The aim of this work was to evaluate the contact toxicity of Pennyroyal mint (Mentha pulegium), oregano (Origanum compactum) and thyme (Thymus capitatus) essential oils against adults, larvae and eggs of two-spotted spider mite Tetranychus urticae Koch (Tetranychidae). The chemical composition of these three plant essential oils was also characterized. Laboratory tests were conducted to determine the acaricidal effect of plant essential oils at several doses in geometric progression. The consequences of treatments on mortality were measured in relationships with concentrations of essential oils. On the other hand, the major constituents of the three essential oils were identified by gas chromatography-mass spectrometer (GC-MS) techniques. GCMS analyses proved that the major compound present in the essential oils of thyme and oregano is carvacrol (55.59 and 55.28% respectively) while the main component of pennyroyal mint essential oil is pulegone (67,63%). Laboratory bioassay results indicated that essential oils of thyme and pennyroyal mint caused the most important acaricidal effects in comparison with the essential oil of oregano. At a concentration of 1%, thyme and pennyroyal eradicate all adults of the mite while oregano causes a mortality of 84.99%. On larvae, the mortality rate of thyme, pennyroyal and oregano is 97.7; 89.47 and 57.89% respectively. The results of the present study concluded that plant essential oils could be useful in promoting research aiming at the development of new agent for pest control from the plants with medicinal values.

scholarly journals CHEMICAL COMPOSITION, ANTIBACTERIAL ACTIVITY OF ESSENTIAL OILS AND LEAF EXTRACTS OF MENTHA PULEGIUM (L) AND GLYCYRRHIZA FOETIDA (DESF) AGAINST THE PHYTOPATHOGENIC BACTERIA

2019 ◽  
Vol 31 (1) ◽  
pp. 47-54
Author(s):  
Hanane Lougraimzi ◽  
Aghraz Abdellah ◽  
Bouaichi Abdelaaziz ◽  
El J. Meryem ◽  
Achbani E. Hassan
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Inhibition Zone ◽  
Antimicrobial Activities ◽  
Minimal Bactericidal Concentration ◽  
Mentha Pulegium ◽  
Leaf Extracts ◽  
Phytopathogenic Bacteria

This research was conducted to evaluate the components of the Mentha pulegium (L) and Glycyrrhiza foetida (Desf) that were extracted by hydro distillation and analyzed using GC-MS methods. They are spontaneous plants widespread in Gharb of Morocco. The aim of the study is to investigate the antibacterial activity of the essential oils and leaf methanolic extracts of these two medicinal plants against five phytopathogenic bacteria. The evaluation of the antimicrobial activity of the two species by estimating the diameter of the inhibition zone has shown that the essential oil of M. pulegium exhibited a higher antimicrobial activity than G. foetida which varied according to the sensitivity of the phytopathogenic strains. The results obtained revealed different degrees of sensitivity toward methnolic extract. However, the methanolic extract and the essential oil of M. pulegium are more active based on the Minimal Inhibitory Concentration (MIC) and Minimal Bactericidal Concentration (MBC). The antimicrobial activities of leaf extracts from the two plants highlighted in this study could justify their therapeutic use. These results should be strongly recommended as an alternative to a chemical compound that still represents the problem of toxic residues.

Hydroxycamptothecin Stealth Liposomes: Containing TPGS as a Novel PEGylated Long-Circulating Coating Material

2014 ◽  
Vol 886 ◽  
pp. 333-336
Author(s):  
Hai Long Wang ◽  
Shan Kui Liu ◽  
Yao Yao Qin ◽  
Yun Gang Chen
Keyword(s):  
Thin Film ◽  
Physicochemical Characteristics ◽  
Release Profile ◽  
Coating Material ◽  
Dispersion Method ◽  
Stealth Liposomes

The goal of this study was to develop long-circulating liposomes of hydroxylcamptothecin (HCPT) with TPGS as a new coating material. Liposomes were prepared by a thin-film dispersion method with the lipids comprised phosphatidylcholine, cholesterol and TPGS. By contrast, other common PEGylated derivatives were also involved. HCPT-loaded liposomes with TPGS as coating material showed similar physicochemical characteristics andin vitrorelease profile to liposomes with DSPE-PEG or DPPE-PEG. It is suggested that TPGS can be applied as a novel coating material for long-circulating liposomes.

scholarly journals Antimicrobial activity of four essential oils extracted from plants commonly used in traditional medicine against some clinical strains

2019 ◽  
Vol 65 (2) ◽  
pp. 22-29
Author(s):  
Said Oulkheir ◽  
Hadia Boumariem ◽  
Hanane Dand ◽  
Mohamed Aghrouch ◽  
Khadija Ounine ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Candida Albicans ◽  
Essential Oil ◽  
Essential Oils ◽  
Pathogenic Bacteria ◽  
Inhibitory Effect ◽  
Diffusion Method ◽  
Mentha Pulegium ◽  
Plant Oil ◽  
Mueller Hinton

Summary Introduction: Recently, efforts regarding the discovery of the effectual components of plants possessing antimicrobial properties are advanced. Herbal essential oils are widely used for treatment of various diseases, and they play an important role in healthcare considerations. Objective: This study aims to evaluate the effectiveness of Cinnamomum verum, Eucalyptus globulus, Lavandula angustifolia and Mentha pulegium essential oils against Candida albicans and some pathogenic bacteria. Methods: The antibacterial activity of four essential oils (EOs) against different microbial strains was evaluated using the disk diffusion method as well as determination of the minimal inhibitory concentration (MIC), and bactericidal concentration (MBC). For Candida albicans, the MFC of the plant oils was determined using a macro broth dilution assay. A range of concentrations (50 to 0.2 mg/ml) were prepared in Mueller Hinton Broth medium in flasks. Tween 80 (0.01% v/v) was included to enhance oil solubility. Each flask was inoculated with 108 CFU/ml of C. albicans. The flasks were incubated at 35°C for 48 hours. From each flask 13 μl of culture was inoculated onto Mueller-Hinton Agar plates and incubated at 35°C for 48 h. The plates were observed and the MFC was determined as the lowest concentration of plant oil completely inhibiting the growth of C. albicans. Results: The obtained results showed that all bacteria and yeasts tested were sensitive to cinnamon essential oil with an inhibition zone ranging from 22 to 39.33 mm and a MIC ranging from 0.20 mg/ml to 1.56 mg/ml. At low concentrations ranging from 0.2 to 3.13 mg/ml, this essential oil has shown the most important bactericidal effect. Eucalyptus essential oil showed the highest inhibitory effect on Staphylococcus aureus with a diameter of 21.33±1.15 mm. The antibacterial effect of mint indicates that the most sensitive bacterium is A. boumannii. However, S. enteritidis, C. albicans, K. pneumoni and P. aeruginosa are resistant germs whose inhibition diameter varies from 7.33±1.15 mm to 11.33±1.15 mm. Lavender EO has an inhibitory effect against S. aureus (20.67±1.15 mm) and an intermediate effect against Streptococcus pyogenes, Serratia marcescens and Enterococcus faecalis. Conclusions: The antibacterial activity of essential oils, especially those of cinnamon against the strains studied, supports their potential use as a remedy against infectious microbial diseases.

scholarly journals Anticancer Effect of Solid-Lipid Nanoparticles Containing Mentha longifolia and Mentha pulegium Essential Oils: In Vitro Study on Human Melanoma and Breast Cancer Cell Lines

2021 ◽  
Vol 12 (2) ◽  
pp. 2128-2137
Keyword(s):  
Breast Cancer ◽  
Essential Oil ◽  
Essential Oils ◽  
Cell Lines ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Solid Lipid Nanoparticles ◽  
Lipid Nanoparticles ◽  
Breast Cancer Cell Lines ◽  
Mentha Pulegium

Breast cancer and melanoma are common cancers with several treatments such as surgery, radiotherapy, chemotherapy, and their side effects. Therefore, new drugs using plant-derived substances (especially essential oils) have received more attention in recent years; however, they are generally less effective than synthetic drugs. Therefore, the preparation of essential oil-based nanoformulations is considered a promising approach to improving their efficiency. In this study, ingredients of Mentha longifolia and Mentha pulegium essential oils were first identified by GC-MS analysis. Their anticancer effects were then evaluated against one melanoma cell line (A-375) and two breast cancer cell lines (MDA-MB-468 and MCF-7). The cytotoxic effect of the essential oil on all cell lines at even the highest concentration, 1200 µg/mL, was not proper (viability > 55%). After that, solid lipid nanoparticles containing each essential oil with particle sizes of 107 ± 9 (PDI 0.274) and 191 ± 8 (PDI 0.174) nm and zeta potential -7.10 and -4.81 mV were prepared. Interestingly, both prepared nanoformulations reduced the viability of all three cell lines to around 10% at half the mentioned concentration, 600 µg/mL. Thus, the prepared nanoformulations could be introduced as proper candidates for investigation in-vivo research and supplementary medicine.

scholarly journals Nanoencapsulation of Mandarin Essential Oil: Fabrication, Characterization, and Storage Stability

Foods ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 54
Author(s):  
Amer Ali Mahdi ◽  
Qais Ali Al-Maqtari ◽  
Jalaleldeen Khaleel Mohammed ◽  
Waleed Al-Ansi ◽  
Sahibzada Muhammad Aqeel ◽  
...  
Keyword(s):  
Essential Oil ◽  
Encapsulation Efficiency ◽  
Storage Stability ◽  
Gum Arabic ◽  
Physicochemical Characteristics ◽  
Protein Isolate ◽  
High Thermal Stability ◽  
Wall Material ◽  
Citrus Reticulata ◽  
And Storage

This study evaluates the combined efficiency of whey protein isolate (WPI) with maltodextrin (MD) and gum arabic (GA), as a delivery system for encapsulating Citrus reticulata essential oil (CEO). The wall materials blended at different rates were produced to obtain seven formulations of nanocapsules (NCEO), namely NCEO-GA, NCEO-MD, NCEO-WPI, NCEO-GA/MD, NCEO-GA/WPI, NCEO-MD/WPI, and NCEO-GA/MD/WPI. The interaction between CEO and WPI was simulated by molecular docking. Findings showed that the physicochemical characteristics and storage stability of formulations containing WPI were considerably improved. The NCEO-GA/MD/WPI formulation demonstrated the optimum values of encapsulation efficiency (92.08%), highest glass transition temperature (79.11 °C), high crystallinity (45.58%), high thermal stability (mass loss at 100 °C < 5%), and also had the highest antioxidant activity and lowest peroxide value after storage. This study demonstrated that combining WPI with MD and GA, as wall material encapsulation, can produce nanocapsules with superior properties to those created using polysaccharides individually.

Chemical Composition and Antibacterial Activity of Essential Oils of Three Medicinal Plants from Algerian Semi-Arid Climatic Zone

2018 ◽  
Vol 16 (S1) ◽  
pp. S155-S163 ◽  
Author(s):  
S. Mehalaine ◽  
O. Belfadel ◽  
T. Menasria ◽  
A. Messaili
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Rosmarinus Officinalis ◽  
Salvia Officinalis ◽  
Thymus Algeriensis

The present study was carried out to determine, for the first time, the chemical composition and antibacterial activity of essential oils derived from the aerial parts of three aromatic plants Thymus algeriensis Boiss & Reut, Rosmarinus officinalis L., and Salvia officinalis L. growing under semiarid conditions. The essential oils were chemically analyzed and identified by gas chromatography (GC) and GC/ mass spectrometry (GC/MS) and their antimicrobial activity was individually evaluated against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa using both agar disk diffusion and agar dilution methods. The major constituents of Thymus algeriensis essential oil were identified as camphor (13.62%), 1,8-cineol (6.00%), borneol (5.74%), viridiflorol (4.00%), and linalool (3.93%). For Rosmarinus officinalis essential oil, 48 compounds were characterized, of which the main constituents were camphor (17.09%), Z-β-ocimene (10.88%), isoborneol (9.68%), α-bisabolol (7.89%), and borneol (5.11%). While, Salvia officinalis essential oil was characterized by β-thujone (16.44%), followed by viridiflorol (10.93%), camphor (8.99%), 1,8-cineol (8.11%), trans-caryophyllene (5.85%), and α-humulene (4.69%) as the major components. Notably, results from antibacterial screening indicated that Thymus algeriensis and Salvia officinalis essential oils exhibited a strong inhibitory effect against both Escherichia coli and Staphylococcus aureus compared to Rosmarinus officinalis essential oil. Further, less activity was recorded against Pseudomonas aeruginosa for the three tested essential oils.

Contribution to the Optimization of a Gas Chromatographic Method by QbD Approach used for Analysis of Essential Oils from Salvia officinalis

2019 ◽  
Vol 70 (6) ◽  
pp. 2015-2020
Author(s):  
Silvia Robu ◽  
Aurelia Romila ◽  
Olimpia Dumitriu Buzia ◽  
Adrian Florin Spac ◽  
Camelia Diaconu ◽  
...  
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Plant Material ◽  
Mobile Phase ◽  
Chromatographic Method ◽  
Salvia Officinalis ◽  
Salvia Officinalis L ◽  
Gas Chromatographic Method

Numerous articles on Salvia officinalis L. have been published regarding the composition of their essential oil. The considerable variation found may be due to the quality of the plant material as well as to the methods used for analysis. A simple GC-MS method was developed and optimized in the QbD approach, for the determination of sage essential oils. The optimization of GC-MS analysis was performed using different mobile phase flows, injection volumes, split ratios and temperature programs. The optimized method proved to be simple and can be successfully applied for the determination of sage essential oils.

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