The Potential Use of Borassus flabellifer Linn. Color Extract as a Natural Dye

2021 ◽  
Vol 901 ◽  
pp. 9-15
Author(s):  
Arpa Petchsomrit ◽  
Nadechanok Jiangseubchatveera ◽  
Nattawut Leelakanok ◽  
Thanchanok Sirirak
Keyword(s):  
Antioxidant Activity ◽  
Light Exposure ◽  
Ethanolic Extract ◽  
Natural Dye ◽  
Carotenoid Content ◽  
Extractive Content ◽  
Color Intensity ◽  
Palm Fruit ◽  
Freeze Dried ◽  
Palm Fruits

Palmyra (Borassus flabellifer Linn.) palm fruits are bright yellow and have a unique sweet scent. The ripe palm fruits are a rich source of carotenoids which are used as a non-toxic natural dye and have been recognized for their antioxidant benefits. This study aimed to explore the potential of palm fruit extract as a natural coloring agent. The ripe mesocarp of B. flabellifer was extracted using various organic solvents. The carotenoid content, polysaccharide composition, and antioxidant activity of the palm extracts were investigated. The ethanolic palm extract was selected for stability study in various conditions (pH, time, light, and temperature). Then, the extracted nanoemulsion loaded with B. flabellifer extract was formulated and evaluated. The ethanolic extract from the freeze-dried sample yielded high extractive content (42.9%) and showed the greatest antioxidant activity (IC50 2.4 mg/mL). The color intensity of the extract did not fade after exposure to a pH of more than 4. After the storage for 14 days, the color intensity significantly decreased. The light exposure did not influence the color intensity at 25°C. B. flabellifer ethanolic extracted nanoemulsions were successfully prepared by ultrasonication method with the oil to surfactant weight ratio of 1:9. The nanoemulsions loaded with ethanolic extract were stable. The nanoemulsion of the pulp extract could potentially be used as a natural dye.

scholarly journals Selection of culture media for the production of carotenoids with antioxidant activity by Rhodotorula glutinis

2018 ◽  
pp. 31-38
Author(s):  
Gerelmaa Z ◽  
Zultsetseg Ch ◽  
Batjargal B ◽  
Rentsenkhand Ts
Keyword(s):  
Antioxidant Activity ◽  
Culture Media ◽  
Antioxidant Properties ◽  
Rhodotorula Glutinis ◽  
Stationary Growth Phase ◽  
Carotenoid Content ◽  
Stationary Growth ◽  
Natural Pigment ◽  
Freeze Dried ◽  
Selection Of

Rhodotorula glutinis is an aerobic yeast with particular metabolic characteristic that can produce large amount of carotenoids during the stationary growth phase. Carotenoid is an important natural pigment with antioxidant properties, which is used in food, pharmaceutical and cosmetics industry as additives. The aim of this work is to study the production of carotenoid with antioxidant activity by R.glutinis R12 in different media conditions. The selected strain was cultivated in three different culture media such as YM broth, Basal and MS3 medium at 28°C for 72–120 hours. After fermentation, cells were harvested by centrifugation and freeze-dried. Carotenoid from the biomass was extracted as a mixture of DMSO, acetone and petroleum ether with a ratio of 1:2:2 and cells were ruptured using ultrasonic wave. The carotenoid content in the supernatant was measured by spectrophotometric method. The highest content of carotenoids extracted from R.glutinis R12 was 283.71µg/g dry biomass. The maximum antioxidant activity of carotenoid by DPPH assays were achieved 52.09 ± 0.4% (IC50= 536.02) and at a concentration of 600 µg ml-1. This study revealed that the R.glutinis R12 strain has the ability to produce carotenoid and has shown antioxidant activity in Basal and MS3 medium. For further study, it is necessary to investigate the improvement of carotenoid yield from R.glutinis R12 strains.

Chemical characterization of ethanolic extract of Butterfly pea flower (Clitoria ternatea)

Food Research ◽  
2021 ◽  
Vol 5 (4) ◽  
pp. 127-134
Author(s):  
T.N.M. Tuan Putra ◽  
M.K. Zainol ◽  
N.S. MohdIsa ◽  
N. MohdMaidin
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Light Exposure ◽  
Ethanolic Extract ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Clitoria Ternatea ◽  
Dpph Method ◽  
Butterfly Pea

Clitoria ternatea, commonly known as Butterfly pea flower or Bunga telang, is a tropical flower and notable as one of the most vital sources of polyphenols with high antioxidant capacity. C. ternatea abundantly produces anthocyanin compounds benefiting natural blue colourants. To date, a blue colourant is the most difficult to obtain since the anthocyanins responsible for this colour is the least stable and easily degraded after extraction. Their stability commonly relies on several operational parameters, such as shifted pH value, high temperature, and light exposure. Apart from colour attributes, anthocyanins have also been identified as a source of functional molecules due to antioxidant activity and beneficial health effects such as anti-cancer, anti-obesity, antimicrobial and more. This study aimed to characterize the chemical constituents of the extractable polyphenols from ethanolic extract of C. ternatea quantitatively. The numerical data were evaluated using the Minitab version 18 statistical method. The chemical profiling, including total phenolic content (TPC), total flavonoid content (TFC), total monomeric anthocyanins (TMA) were determined using standard methods. The antioxidant activity was evaluated using DPPH and ABTS methods. The results showed that the ethanolic extract of C. ternatea had 35.7 mg QE/g of TFC, 102.4 mg GAE/g280nm and 28.8 mg GAE/g750nm of TPC and 2.7 CE/g and 2.9 ME/g of TMA. The total phenolic content of C. ternatea showed a good correlation with the antioxidant activity by the DPPH method when analysed using Pearson correlation and showed an increasing trend of antioxidant power with regards to TPC in both assays. In a nutshell, this study contributes to the knowledge of ethanolic extract of C. ternatea. Future research could consider exploring other extraction methods that could enhance the stability of polyphenols particularly the anthocyanins from degradation such as using surfactants.

scholarly journals Sea Buckthorn Leaf Powders: The Impact of Cultivar and Drying Mode on Antioxidant, Phytochemical, and Chromatic Profile of Valuable Resource

Molecules ◽  
2021 ◽  
Vol 26 (16) ◽  
pp. 4765
Author(s):  
Lina Raudone ◽  
Viktorija Puzerytė ◽  
Gabriele Vilkickyte ◽  
Aurelija Niekyte ◽  
Juozas Lanauskas ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Chlorophyll Content ◽  
Antioxidant Activities ◽  
Sea Buckthorn ◽  
Carotenoid Content ◽  
Array Detector ◽  
Quantitative Composition ◽  
Antioxidant Power ◽  
Freeze Dried ◽  
The Impact

Sea buckthorn (Hippophae rhamnoides L. (HR)) leaf powders are the underutilized, promising resource of valuable compounds. Genotype and processing methods are key factors in the preparation of homogenous, stable, and quantified ingredients. The aim of this study was to evaluate the phenolic, triterpenic, antioxidant profiles, carotenoid and chlorophyll content, and chromatic characteristics of convection-dried and freeze-dried HR leaf powders obtained from ten different female cultivars, namely ‘Avgustinka’, ‘Botaniceskaja Liubitelskaja’, ‘Botaniceskaja’, ‘Hibrid Percika’, ‘Julia’, ‘Nivelena’, ‘Otradnaja’, ‘Podarok Sadu’, ‘Trofimovskaja’, and ‘Vorobjovskaja’. The chromatic characteristics were determined using the CIELAB scale. The phytochemical profiles were determined using HPLC-PDA (high performance liquid chromatography with photodiode array detector) analysis; spectrophotometric assays and antioxidant activities were investigated using ABTS (2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) and FRAP (ferric ion reducing antioxidant power) assays. The sea buckthorn leaf powders had a yellowish-green appearance. The drying mode had a significant impact on the total antioxidant activity, chlorophyll content, and chromatic characteristics of the samples; the freeze-dried samples were superior in antioxidant activity, chlorophyll, carotenoid content, and chromatic profile, compared to convection-dried leaf powder samples. The determined triterpenic and phenolic profiles strongly depend on the cultivar, and the drying technique had no impact on qualitative and quantitative composition. Catechin, epigallocatechin, procyanidin B3, ursolic acid, α-amyrin, and β-sitosterol could be used as quantitative markers in the phenolic and triterpenic profiles. The cultivars ‘Avgustinka’, ‘Nivelena’, and ‘Botaniceskaja’ were superior to other tested cultivars, with the phytochemical composition and antioxidant activity.

Screening of Microalgae for Antioxidant Activities, Carotenoids and Phenolic Contents

2014 ◽  
Vol 625 ◽  
pp. 156-159 ◽  
Author(s):  
Hamdy Elsayed Ahmed Ali ◽  
Sanaa Mahmoud Metwally Shanab ◽  
Mervat Aly Mohamed Abo-State ◽  
Emad Ahmed Ahmed Shalaby ◽  
Usama Eldmerdash ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Scenedesmus Obliquus ◽  
Ethanolic Extract ◽  
Natural Antioxidants ◽  
Carotenoid Content ◽  
Total Phenolic ◽  
Microalgal Biomass ◽  
Phenolic Contents ◽  
Dpph Scavenging

Ten different microalgal species were screened for their antioxidant activity using 2,2 ́-diphenyl-1-picrylhydrazyl (DPPH) scavenging assay, both total phenolic and carotenoid contents were determined. The ethanolic extract ofOscillatoriasp. showed the highest antioxidant activity (69.1 %), while the greenChlorellasp. showed higher phenolic content (39.1 mg GAE g-1dry wt.) andScenedesmus obliquusrecorded higher carotenoid content (3.73 mg L-1), than cyanobacterial species Microalgal biomass plays a great role as a potential source of natural antioxidants, not only due to the carotenoids but also the phenolic compounds.

scholarly journals Nutritional and functional properties of dried Momordica cochinchinensis Spreng. fruit aril and its application in cheese spread production

Food Research ◽  
2020 ◽  
Vol 4 (6) ◽  
pp. 1947-1953
Author(s):  
A.F.A. Carbonera ◽  
R.R.P. Tayobong ◽  
L.I.C. Barrameda ◽  
A.M.V. Miranda
Keyword(s):  
Antioxidant Activity ◽  
Vitamin A ◽  
Vitamin A Deficiency ◽  
The Philippines ◽  
Carotenoid Content ◽  
Drying Methods ◽  
Nutritional Properties ◽  
Freeze Dried ◽  
Momordica Cochinchinensis ◽  
Β Carotene

Momordica cochinchinensis Spreng. is an underutilized fruit-bearing indigenous plant in the Philippines with great potential due to its reported nutritional properties. The aril portion of the fruit was used because of its high carotenoid content. The aril was dried using oven drying (60°C) and freeze drying methods to a final moisture content of 15%, and then compared in terms of its lycopene, β-carotene, and Vitamin A contents as well as antioxidant activity. To determine the potential of the dried aril as an additive to produce a fortified product, it was added at an amount of 4 g per 100 g of cheese spread then its nutritional properties were evaluated. Results showed that freeze dried aril has high βcarotene (2090.28 µg/g) and Vitamin A (3483.80 IU/g) but is not significantly different than the oven-dried aril while the oven-dried aril has significantly higher lycopene content (405.22 µg/g) than the freeze dried aril (325.84 µg/g). Furthermore, the freeze dried aril has significantly higher antioxidant activity (29.81%) than the oven-dried aril (16.27%). The addition of the dried aril to cheese spread resulted to a product with significantly higher lycopene (30.15 µg/g), β-carotene (24.93 µg/g), Vitamin A (41.55 IU/g), and Vitamin C (1.20 mg/100 g) compared to the cheese spread with no aril powder. Thus, a serving size of 1 tbsp (20 g) of the cheese spread with aril powder can provide 17% daily value for Vitamin A and has satisfied the definition of fortified food as well as the USDA standard reference for Vitamin A. This functional ingredient can therefore address concerns on Vitamin A deficiency.

UJI AKTIVITAS ANTIOKSIDAN FRAKSI N-HEKSAN DAUN AFRIKA (Vernonia amygdalina Del.) DENGAN METODE DPPH

2020 ◽  
Vol 5 (2) ◽  
pp. 250-257
Author(s):  
Nurul Fatimah ◽  
◽  
Reksi Sundu
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Free Radicals ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Hexane Fraction ◽  
Phytochemical Screening ◽  
Vernonia Amygdalina ◽  
Tropical Africa ◽  
Ic50 Value

Free radicals and reactive species are widely believed to contribute to the development of several diseases by causing oxidative stress and eventually oxidative. Vernonia amygdalina (Astereacea) is a small shrub or tree between 1 and 5m high growing throughout tropical Africa. Plants are generally known as bitter leaves is well cultivated and is a general market for merchandise in several countries. The purpose of this study was to determine the antioxidant activity of hexane fraction from ethanol extract od Frican leaves (Vernonia amygdalina Del.). The method used in this study was the DPPH (1,1-Diphenil-2-Picrylhydrazyl) method. The result of phytochemical screening showed that ethanolic extract of African leaves contained a composition of secondary metabolites of alkaloids, flavonoids, tannins, steroids/triterpenoids and saponins. The antioxidant activity of the extract of n-hexane fraction was classified as very weak with an IC50 value of 317.98 ppm.

Synergistic action of propolis with levodopa in the management of Parkinsonism in Drosophila melanogaster

2020 ◽  
Vol 17 (3) ◽  
Author(s):  
Emmanuel Tiyo Ayikobua ◽  
Josephine Kasolo ◽  
Keneth Iceland Kasozi ◽  
Ejike Daniel Eze ◽  
Abass Safiriyu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Drosophila Melanogaster ◽  
Side Effects ◽  
Motor Activity ◽  
Ethanolic Extract ◽  
Synergistic Action ◽  
Content Type ◽  
Treatment Groups ◽  
Ethanolic Extract Of Propolis ◽  
Different Doses

AbstractBackgroundThe Phosphatase and tensin-induced putative kinase 1 (PINK1B9) mutant for Drosophila melanogaster is a key tool that has been used in assessing the pathology of Parkinsonism and its possible remedy. This research was targeted toward determining the effects of ethanolic extract of propolis, with levodopa therapy in the management of Parkinsonism.MethodThe PINK1B9 flies were divided into groups and fed with the different treatment doses of ethanoic extract of propolis. The treatment groups were subjected to 21 days of administration of propolis and the levodopa at different doses after which percentage climbing index, antioxidant activity and lifespan studies were done.ResultsPropolis alone improved motor activity, antioxidant and lifespan in Drosophila melanogaster than in PINK1 flies. Propolis in combination with levodopa significantly (P<0.05) improved physiological parameters at higher than lower concentrations in Parkinsonism Drosophila melanogaster demonstrating its importance in managing side effects associated with levodopa.ConclusionPropolis is a novel candidate as an alternative and integrative medicinal option to use in the management of Parkinsonism in both animals and humans at higher concentrations.

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