The Effect of Different Extracting Conditions on the Antibacterial Activity of Moringa oleifera Lam. Leaves Extract for the Development of Antibacterial Meat Tray

Moringa oleifera Lam. leaves is the popular plant which provides an impressive range of medicinal uses because of major phytoconstituents in leaves. Such phytoconstituents contents are highly affected by extraction conditions. Therefore, the effects of different solvent extraction, temperature and time of extraction on antibacterial activity and total phenolic contents (TPC) of M.oleifera Lam. leaves extract were determined. The antibacterial result of all extract against E.coli showed no inhibition zone, while ethanolic extracts significantly affected to S.aureus inhibition that increasing concentration of ethanol provided an increasing in inhibition zone and decreasing MIC value from 5 to 1.25 mg/ml. Low-temperature extraction (40°C) was indicated as the optimum temperature which led to high antibacterial activity. The effect of extraction time was directly related to the inhibition zone. The highest inhibition zone and MIC value were obtained from extract which was extracted by using 70%MeoH, 40OC and 3 h. TPC result revealed a significant relationship with antibacterial activity, meaning that higher TPC led to higher antibacterial inhibition.

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Endophytic Actinobacteria from Rhododendron spp. as an Antibacterial Agent

Biosaintifika Journal of Biology & Biology Education ◽

10.15294/biosaintifika.v9i3.10323 ◽

2017 ◽

Vol 9 (3) ◽

pp. 600

Author(s):

Risa Fitriandini ◽

Sri Budiarti ◽

Yulin Lestari

Keyword(s):

Antibacterial Activity ◽

Antibacterial Agent ◽

Bacillus Pumilus ◽

Inhibitory Concentration ◽

Bacterial Pathogen ◽

Inhibition Zone ◽

Cell Number ◽

High Antibacterial Activity ◽

Mic Value ◽

Endophytic Actinobacteria

Rhododendron has long been known to treat various diseases including diarrhea, but diversity and potency of its endophytic actinobacteria has not been studied. The objectives of this research were to explore the existence of endophytic actinobacteria from Rododendron spp. and assesed their antibacterial activity, as an effort to control the growth of bacterial pathogen resistant to some antibiotics. The endophytes were isolated from Rhododendron spp. using HV medium, and purified in ISP2 medium. The antibacterial activity was assayed against Enteropathogenic Escerichia coli (EPEC) K1.1 resistant to ampicillin and Bacillus pumilus. The Minimum Inhibitory Concentration (MIC) value, macroscopic and microscopic were examined. Twenty three of endophytic actinobacteria were successfully isolated from 7 Rhododendron species. Two of them, i.e. RJkb1 and RJkb3 isolates, had high antibacterial activity, with 17.2 mm and 14.5 mm inhibition zone against EPEC K1-1, respectively; and 12.4 mm and 16.1 mm inhibition zone against B. pumilus, respectively. The highest antibacterial activity for both RJkb1 and RJkb3 isolates was achieved at day 15, at 28 oC. At 250 µg/mL to 1750 µg/mL either RJkb1 or RJkb3 supernatant showed no activity against EPEC K1-1. The MIC value against B. pumilus was at 1250 µg/mL for both tested isolates. Under an electron microscope observation, cell morphology of the treated B. pumilus showed elongated cells and viewer in cell number, compared with the untreated one. From this work, the existence of endophytic actinobacteria from Rhododendron spp. and their antibacterial activity contribute to the understanding of their diversity and potency as antibacterial agent.

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Bioactive Compounds, Antioxidant, and Antibacterial Properties of Lonicera caerulea Berries: Evaluation of 11 Cultivars

Plants ◽

10.3390/plants10040624 ◽

2021 ◽

Vol 10 (4) ◽

pp. 624

Author(s):

Laima Česonienė ◽

Juozas Labokas ◽

Ina Jasutienė ◽

Antanas Šarkinas ◽

Vilma Kaškonienė ◽

...

Keyword(s):

Antibacterial Activity ◽

Vitamin C ◽

Organic Acids ◽

Bioactive Compounds ◽

Total Phenolic ◽

Aqueous Extracts ◽

Lonicera Caerulea ◽

Phenolic Contents ◽

Total Phenolic Contents ◽

Ethanolic Extracts

The aim of the study was to evaluate 11 cultivars of blue honeysuckle (Lonicera caerulea L.) for bioactive compounds, antioxidant capacity, and the antibacterial activity of berries. Total phenolic contents (TPCs) and total anthocyanin contents (TACs) were established by using ethanolic extracts. For contents of organic acids and saccharides, aqueous extracts were used, and vitamin C was determined by using oxalic acid solution. DPPH• radical scavenging capacity was evaluated by using ethanolic extracts; antibacterial activity was assessed by using both ethanolic and aqueous extracts. The TPC varied from 364.02 ± 0.41 mg/100 g in ‘Vostorg’ to 784.5 ± 0.3 mg/100 g in ‘Obilnaja’, and TAC ranged from 277.8 ± 1.1 mg/100 g in ‘Čelnočnaja’ to 394.1 ± 8.4 mg/100 g in ‘Nimfa’. Anthocyanins comprised 53.8% of total phenolic contents on average. Among organic acids, citric acid was predominant, averaging 769.41 ± 5.34 mg/100 g, with malic and quinic acids amounting to 289.90 ± 2.64 and 45.00 ± 0.37 mg/100 g on average, respectively. Contents of vitamin C were 34.26 ± 0.25 mg/100 g on average. Organic acids were most effective in the inhibition of both Gram-positive and Gram-negative bacteria tested. In conclusion, berries of L. caerulea are beneficial not only for fresh consumption, but also as a raw material or ingredients of foods with high health-promoting value.

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Antimicrobial activity of Terminalia catappa brown leaf extracts against Staphylococcus aureus ATCC 25923 and Pseudomonas aeruginosa ATCC 27853

F1000Research ◽

10.12688/f1000research.15998.1 ◽

2018 ◽

Vol 7 ◽

pp. 1406 ◽

Cited By ~ 5

Author(s):

Ovin Qonita Allyn ◽

Eko Kusumawati ◽

Rudy Agung Nugroho

Keyword(s):

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Antibacterial Activity ◽

Inhibition Zone ◽

Total Phenolic ◽

Inhibition Zone Diameter ◽

Terminalia Catappa ◽

Ethanolic Extracts ◽

High Concentrations ◽

Dried Extract

The aim of this study was to determine the effects of various concentration ofTerminalia catappabrown leaves extract which can inhibit the growth ofStaphylococcus aureusATCC 25923 andPseudomonas aeruginosaATCC 27853. The crushed-brown leaves ofTerminalia catappawas extracted using 95% ethanol, filtered, and evaporated. The driedT. catappaextract was used to identify phytochemical content qualitatively. Total phenolic and flavonoid contents were also measured quantitatively from dried extract. The dried extracts were also dissolved in sterile aquadest and serial dilutions were prepared to final concentration of 30, 60 and 90%. A disc diffusion method was used to evaluate the antibacterial activity of various concentrations of ethanol extract of brown leaves ofT. catappa. Inhibition zone diameter was measured to determine antibacterial activity. Gentamycin sulfate and distilled water were used as positive and negative controls, respectively. Dried ethanolic extract of brownT. catappaleaves contained flavonoid, quinon, phenolic, triterpenoid, and tannin. A total of 208.722 mg gallic acid equivalent/g extract of total phenolic and 35.7671 mg quercetin equivalent/g extract of total flavonoid were also found in the dried extract. The inhibition zone diameters of ethanolic extracts ranged from 1.73 to 9.06 mm (S. aureus) and from 1.83 to 6.5 mm (P. aeruginosa). The higher concentration of extract, the wider the inhibition zone diameters for both bacteria.P. aeruginosawas more resistant to high concentrations of extract (90%) thanS. aureus. Ethanolic extracts of the brown leaves ofT. catappahad different antibacterial effects againstS. aureusandP. aeruginosa. The higher the concentration of extract, the wider the inhibition zone diameter for both bacteria.P. aeruginosawas more resistant to high concentrations of ethanolic extracts of the brown leaves ofT. catappa.

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Quercetin as a Precursor for the Synthesis of Novel Nanoscale Cu (II) Complex as a Catalyst for Alcohol Oxidation with High Antibacterial Activity

Bioinorganic Chemistry and Applications ◽

10.1155/2021/8818452 ◽

2021 ◽

Vol 2021 ◽

pp. 1-9

Author(s):

Zahra Moodi ◽

Ghodsieh Bagherzade ◽

Janny Peters

Keyword(s):

Antibacterial Activity ◽

Schiff Base ◽

Biological Activities ◽

Schiff Base Complex ◽

13C Nmr Spectroscopy ◽

Inhibition Zone ◽

Gram Negative Bacteria ◽

Gram Positive ◽

Gram Negative ◽

High Antibacterial Activity

Quercetin (3,3′,4′,5,7-pentahydroxyflavone) is one of the dietary flavonoids, distributed in medicinal plants, vegetables, and fruits. Quercetin has the ability to bind with several metal ions to increase its biological activities. In the last two decades, quercetin has attracted considerable attention due to the biological and pharmaceutical activities such as antioxidant, antibacterial, and anticancer. In the present study, quercetin and ethanolamine were used for the synthesis Schiff base complex, which was characterized by IR, 1H NMR, and 13C NMR spectroscopy. The Schiff base has been employed as a ligand for the synthesis of novel nanoscale Cu (II) complex. The product was characterized by FT-IR spectroscopy, FESEM, and XRD. Significantly, the product showed remarkable catalytic activity towards the oxidation of primary and secondary alcohols. The antibacterial activity of the final product was assessed against Staphylococcus aureus (Gram‐positive) and Escherichia coli (Gram‐negative) bacteria using an inhibition zone test. The synthesized nanoscale Cu (II) complex exhibited a strong antibacterial activity against both Gram-positive and Gram-negative bacteria.

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Effect of Steam Blanching, Dehydration Temperature & Time, on the Sensory and Nutritional Properties of a Herbal Tea Developed from Moringa oleifera Leaves

International Journal of Food Science ◽

10.1155/2020/5376280 ◽

2020 ◽

Vol 2020 ◽

pp. 1-11

Author(s):

Yasara W. H. Wickramasinghe ◽

Indira Wickramasinghe ◽

Isuru Wijesekara

Keyword(s):

Moringa Oleifera ◽

Total Phenolic ◽

Sri Lankan ◽

Herbal Tea ◽

Nutritional Properties ◽

Phenolic Contents ◽

Trained Panel ◽

Two Samples ◽

Moringa Oleifera Leaves ◽

Dpph Scavenging

The core purpose of the current study is to explore the use of Moringa oleifera leaves, to produce a herbal tea with acceptable sensory properties and nutritional properties by utilizing the steam blanching technique, different dehydration temperatures and time, which can be accepted in the Sri Lankan market. Six sets of samples were prepared where temperature and time combinations were 55°C—6 h, 60°C—4.30 h, 65°C—3 h for the unblanched samples & 55°C—6 h, 60°C—5.30 h & 65°C—4 h for the steam blanched samples. These samples were evaluated, employing a trained panel of 5 tea tasters and a semi trained panel of 35 members. The sample code 706 (steam blanched, 65°C—4 h) was selected as the sample with best sensory attributes. The blanched and unblanched samples dried at 65°C were tested for their proximate, mineral, vitamin, antioxidant and phytochemical contents. The effects of steam blanching on these two samples were evaluated & compared. This study highlights that steam blanching significantly increased the carbohydrates, fat, Mn, Fe, vitamin A, vitamin E and the DPPH scavenging activity whereas steam blanching significantly reduced the protein, fiber, Na, K, Ca, Total phenolic contents and flavonoids content but vitamin C, Zn, Cu and Mg contents were unaffected by steam blanching.

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Moringa oleifera Supplementation Improves Antioxidant Status and Biochemical Indices by Attenuating Early Pregnancy Stress in Beetal Goats

Frontiers in Nutrition ◽

10.3389/fnut.2021.700957 ◽

2021 ◽

Vol 8 ◽

Author(s):

Ali Afzal ◽

Tarique Hussain ◽

Amjad Hameed

Keyword(s):

Early Pregnancy ◽

Antioxidant Status ◽

Moringa Oleifera ◽

Basal Diet ◽

Total Phenolic ◽

Control Group ◽

Phenolic Contents ◽

Beneficial Effects ◽

Blood Biochemical ◽

Biochemical Indices

This study investigated the effects of supplementing different levels of Moringa oleifera leaf powder (MOLP) on antioxidant status and blood biochemical indices during early gestation in Beetal goats. A total of 30 goats were randomly divided into three groups (n = 10) comprising control (basal diet without MOLP), the 1.6% MOLP supplemented group (basal diet + 1.6% MOLP), and the 3.2% MOLP supplemented group (basal diet + 3.2% MOLP). The experiment started 5 days before estrus synchronization and lasted till day 60 of gestation. The MOLP significantly increased plasma flavonoids in 1.6% as well as 3.2% supplemented group on days 40 and 60 of pregnancy, while total phenolic contents were observed to be higher in the 3.2% MOLP supplemented group throughout the experiment in comparison with the control group. The supplementation improved plasma total antioxidant capacity (TAC) by decreasing malondialdehyde (MDA) and total oxidant status (TOS) in contrast to the control group. The activities of superoxide dismutase (SOD) and peroxidase (POD) were enhanced in both supplemented groups, whereas catalase (CAT) activity was noticed to be significantly high in the 3.2% MOLP supplemented group. The protein contents were significantly elevated with 1.6 and 3.2% supplementation levels from day 40 to day 60 of the experiment. Plasma sugar level, carotenoids, progesterone profile, and hydrolytic (protease and amylase) enzymes activities were improved only when supplemented with 3.2% MOLP. The findings suggest that supplementing with 3.2% MOLP provides beneficial effects on early pregnancy stress in Beetal goats.

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Aktivitas Antibakteri Ekstrak Metanol dari Kulit Batang Kelor (Moringa oleifera Lam.) Terhadap Bakteri Staphylococcus aureus dan Escherichia coli

KOVALEN ◽

10.22487/kovalen.2020.v6.i1.12854 ◽

2020 ◽

Vol 6 (1) ◽

pp. 34-38

Author(s):

Nurul Cholifah ◽

Ahmad Ridhay ◽

Pasjan Satrimafitrah ◽

Ruslan ◽

Hardi Ys

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Moringa Oleifera ◽

Methanol Extract ◽

Inhibition Zone ◽

Stem Bark ◽

Diffusion Method ◽

Bark Extract ◽

Moringa Oleifera Lam

Antibacterial activity of Moringa oleifera Lam. stem bark has been tested against Staphylococcus aureus and Escherichia coli. The extraction of Moringa oleifera Lam. stem bark was used maceration method with methanol solvent and has obtained extract yield of 6.1%. The antibacterial activity test of Moringa oleifera stem bark extracts used a well diffusion method. The concentration of Moringa oleifera stem bark extract was varied to four concentrations of 1% 2% 3% 4% (w/v). The inhibition zone of methanol extract of Moringa oleifera stem bark against Staphylococcus aureus at extract concentrations of 1%, 2%, 3%, and 4% was 10.08 mm, 11.8 mm, 15.00 mm, and 17.02 mm, respectively. The methanol extract of Moringa oleifera stem bark at concentrations of 1%, 2%, 3%, and 4% could also inhibition of Escherichia coli growth with inhibition zone of 14.01 mm, 16.50 mm, 17.09 mm, and 17.10 mm, respectively. Keywords: Moringa Oleifera Lam., Antibacterial, Staphylococcus aureus, Escherichia coli

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Efek Antibakteri Ekstrak Etanol Daun Kelor (Moringa oleifera Lam.) dan Sediaan Masker Gel ¬Peel-Off Ekstrak Etanol Daun Kelor (Moringa oleifera Lam.)

Jurnal MIPA ◽

10.35799/jmuo.8.3.2019.25778 ◽

2019 ◽

Vol 8 (3) ◽

pp. 112

Author(s):

Theresia H. Tunas ◽

Hosea Jaya Edy ◽

Jainer Pasca Siampa

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Moringa Oleifera ◽

Ethanol Extract ◽

Inhibition Zone ◽

Inhibition Zone Diameter ◽

Zone Diameter ◽

Moringa Oleifera Leaves ◽

Inhibitory Power ◽

Moringa Oleifera Lam

Daun Kelor (Moringa oleifera Lam.) bermanfaat untuk menghambat aktivitas pertumbuhan bakteri karena adanya kandungan senyawa flavonoid , alkaloid, dan fenol. Penelitian ini bertujuan untuk menguji aktivitas antibakteri dari ekstrak etanol daun Kelor dan sediaan masker gel peel-off ekstrak etanol daun Kelor terhadap bakteri Staphylococcus aureus. Penelitian ini menggunakan metode eksperimental laboratorium, ekstrak etanol daun kelor dan sediaan masker gel peel-off ekstrak etanol daun Kelor dibuat dengan konsentrasi 1, 3, 5, 7, dan 9%. Ekstrak daun Kelor diperoleh dengan cara maserasi dengan menggunakan pelarut etanol 96%. Pada pengujian antibakteri menggunakan metode sumuran. Diameter zona hambat paling besar yang ditimbulkan oleh ekstrak etanol daun Kelor yaitu pada konsentrasi 7% dengan diameter zona hambat sebesar 5,75 mm, sedangkan pada sediaan masker gel peel-off tidak menunjukkan adanya daya hambat. Dapat disimpulkan bahwa ekstrak etanol daun Kelor memiliki aktivitas antibakteri yang termasuk dalam kategori sedang.Moringa oleifera Lam. (Moringa oleifera) leaves are useful for inhibiting bacterial growth activity due to the presence of flavonoid, alkaloid, and phenol compounds. This study aims to test the antibacterial activity of the Moringa leaf ethanol extract and the gel peel-off mask preparation of Moringa leaf ethanol extract against Staphylococcus aureus bacteria. This study uses laboratory experimental methods, Moringa leaf ethanol extract and gel mask peel-off preparation for Moringa leaf ethanol extract made with concentrations of 1, 3, 5, 7, and 9%. Moringa leaf extract was obtained by maceration using 96% ethanol solvent. In antibacterial testing using the well method. The biggest inhibition zone diameter caused by ethanol extract of Moringa leaves is at a concentration of 7% with a inhibition zone diameter of 5.75 mm, whereas the peel-off gel mask preparation did not show any inhibitory power. It can be concluded that the ethanol extract of Moringa leaves has antibacterial activity which is included in the medium category.

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Antimicrobial, Antioxidant Activities and Total Phenolic Contents of the Traditional Turkish Beverages Produced by Using Grapes

Turkish Journal of Agriculture - Food Science and Technology ◽

10.24925/turjaf.v7isp1.119-125.2774 ◽

2019 ◽

Vol 7 (sp1) ◽

pp. 119

Author(s):

Gülten Tiryaki Gündüz ◽

Ayça Korkmaz ◽

Ezgi Solak ◽

Hande Didar Sözbir

Keyword(s):

Antioxidant Activities ◽

Grape Juice ◽

Total Phenolic ◽

Inhibitory Effects ◽

Antimicrobial Effects ◽

E Coli ◽

Phenolic Contents ◽

Total Phenolic Contents ◽

Antioxidant Agents ◽

Mic Value

In the present study, antimicrobial effects of traditional beverages produced from grapes such as traditional grape pickles, grape juice, home-made hardaliye, and commercially produced hardaliye were investigated using microdilution method against Staphylococcus aureus ATCC 6538P, Escherichia coli ATCC 25922, Salmonella Typhimurium NRRL B4420, Bacillus cereus ATCC 10876, Saccharomyces cerevisiae NRRL Y-12632, Lactobacillus acidophilus La-5 and L. rhamnosus LGG. In addition, total phenolic content was measured by the Folin-Ciocalteau method, and antioxidant activities of grape products were evaluated using DPPH assay. Results showed that grape containing beverages have antimicrobial effects on S. aureus, E. coli, S. typhimurium and B. cereus at various minimum inhibitory concentration (MIC) values in the range of 4.53-150 mg/mL. The lowest MIC value of 4.53 mg/mL was obtained against E. coli for home-made hardaliye. MIC values of the traditional grape pickles were determined as 4.69 mg/mL and 9.38 mg/mL against S.aureus and E.coli, respectively. On the other hand, traditional grape pickles showed weak inhibitory effects against B.cereus with MIC value of 150 mg/mL. The bactericidal effect of these grape products was not detected for any of the test microorganisms however traditional foods produced by using grapes were showed inhibitory effects at different concentrations against tested microorganisms except for probiotics and the yeast. The total phenolic contents of the grape products were within the range of 865.27-2193.08 mg gallic acid equivalent (GAE)/L. Free radical scavenging activities of grape samples ranged from 46% to 90% and the grape juice was found to have the highest antioxidant activity. In conclusion, grape beverages have the potential to act as a antimicrobial and antioxidant agents for use as a natural antimicrobial and antioxidant product in the food industry.

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Gümüşhane İlinde Tıbbi Amaçla Kullanılan Atkuyruğu (Equisetum arvense) Bitkisinin Bazı Biyolojik Aktivitelerinin İncelenmesi

Turkish Journal of Agriculture - Food Science and Technology ◽

10.24925/turjaf.v5i13.1810-1814.1732 ◽

2018 ◽

Vol 5 (13) ◽

pp. 1810

Author(s):

Tuba Acet ◽

Kadriye Özcan

Keyword(s):

Antimicrobial Activity ◽

Antioxidant Activities ◽

Ethanol Extract ◽

Total Phenolic ◽

Equisetum Arvense ◽

Phenolic Contents ◽

Trolox Equivalent ◽

Ethanol Extracts ◽

Gallic Acid Equivalent ◽

Mic Value

In this study, total phenolic content, antimicrobial and antioxidant activities of the extracts obtained with different polarity solvents of horsetail (Equisetum arvense) plant, which is being used by people in Gümüşhane province for different medical purposes, were investigated. Total phenolic contents were measured by spectrophotometric method as gallic acid equivalent. Antimicrobial activity was determined by disc diffusion and microdilution (MIC value) methods and antioxidant activity was determined by ABTS [2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt] and DPPH (2,2-diphenyl-1-picrylhydrazyl) methods and calculated as trolox equivalent. The highest content of phenolic was detected in the ethyl acetate extract (108.9 mg GAE/g extract), while the highest antioxidant capacity was found 15.76 μg/ml as trolox equivalent in the ethanol extract. In addition, the highest antimicrobial activity was detected in the ethanol extracts against to MRSA with 4 μg/ml MIC value.

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