scholarly journals Comparison of serum levels of hepcidin and pro-hepcidin in hemodialysis patients and healthy subjects

2015 ◽  
Vol 26 (1) ◽  
pp. 34 ◽  
Author(s):  
HR Joshaghani ◽  
N Taheri ◽  
Gh Roshandel ◽  
M Mojerloo ◽  
M Hadad ◽  
...  
Keyword(s):  
Healthy Subjects ◽  
Serum Levels ◽  
Hemodialysis Patients

scholarly journals The Effect of Bilateral Nephrectomy on Renalase and Catecholamines in Hemodialysis Patients

2021 ◽  
Vol 18 (12) ◽  
pp. 6282
Author(s):  
Magda Wiśniewska ◽  
Natalia Serwin ◽  
Violetta Dziedziejko ◽  
Małgorzata Marchelek-Myśliwiec ◽  
Barbara Dołęgowska ◽  
...  
Keyword(s):  
Healthy Subjects ◽  
Plasma Concentrations ◽  
Serum Levels ◽  
Hemodialysis Patients ◽  
Pressure Regulation ◽  
Serum Concentrations ◽  
Monoamine Oxidase Activity ◽  
Normal Kidney ◽  
Bilateral Nephrectomy ◽  
Arterial Blood

Background/Aims: Renalase is an enzyme with monoamine oxidase activity that metabolizes catecholamines; therefore, it has a significant influence on arterial blood pressure regulation and the development of cardiovascular diseases. Renalase is mainly produced in the kidneys. Nephrectomy and hemodialysis (HD) may alter the production and metabolism of renalase. The aim of this study was to examine the effect of bilateral nephrectomy on renalase levels in the serum and erythrocytes of hemodialysis patients. Methods: This study included 27 hemodialysis patients post-bilateral nephrectomy, 46 hemodialysis patients without nephrectomy but with chronic kidney disease and anuria and 30 healthy subjects with normal kidney function. Renalase levels in the serum and erythrocytes were measured using an ELISA kit. Results: Serum concentrations of renalase were significantly higher in post-bilateral nephrectomy patients when compared with those of control subjects (101.1 ± 65.5 vs. 19.6 ± 5.0; p < 0.01). Additionally, renalase concentrations, calculated per gram of hemoglobin, were significantly higher in patients after bilateral nephrectomy in comparison with those of healthy subjects (994.9 ± 345.5 vs. 697.6 ± 273.4, p = 0.015). There were no statistically significant differences in plasma concentrations of noradrenaline or adrenaline. In contrast, the concentration of dopamine was significantly lower in post-nephrectomy patients when compared with those of healthy subjects (116.8 ± 147.7 vs. 440.9 ± 343.2, p < 0.01). Conclusions: Increased serum levels of renalase in post-bilateral nephrectomy hemodialysis patients are likely related to production in extra-renal organs as a result of changes in the cardiovascular system and hypertension.

Detection and Quantification of 7-Hydroxydehydroepiandrosterone Epimers in Three Body Fluids

2002 ◽  
Vol 67 (1) ◽  
pp. 10-18 ◽  
Author(s):  
Richard Hampl ◽  
Martin Hill ◽  
Luboslav Stárka
Keyword(s):  
Healthy Subjects ◽  
Serum Levels ◽  
Body Fluids ◽  
Gas Chromatography Mass Spectrometry ◽  
Specific Radioimmunoassay ◽  
Order Of Magnitude ◽  
Three Body ◽  
First Time ◽  
Nanomolar Range ◽  
Detection And Quantification

3β,7α-Dihydroxyandrost-5-en-17-one (1) (7α-OH-DHEA) and its 7β-hydroxy epimer 2 (7β-OH-DHEA) - 7α- and 7β-hydroxydehydroepiandrosterone - were detected and quantified in three human body fluids: in blood serum, saliva and ejaculate. Specific radioimmunoassay and gas chromatography-mass spectrometry have been used. For the first time the data on changes of these dehydroepiandrosterone metabolites are reported for a representative group of healthy subjects of both sexes (172 females and 217 males) during the life span. The serum levels of both 7-hydroxydehydroepiandrosterone epimers in serum and also in semen were in the low nanomolar range, while concentrations by one order of magnitude lower were found in saliva, but still within the detection limit. The results will serve as a basis for comparative studies of 7-hydroxydehydroepiandrosterone levels under various pathophysiological conditions, with a particular respect to autoimmune disorders.

scholarly journals Lipocalin 2 serum levels correlate with age and bone turnover biomarkers in healthy subjects but not in postmenopausal osteoporotic women

Bone Reports ◽  
2021 ◽  
pp. 101059
Author(s):  
Antonio Maurizi ◽  
Marco Ponzetti ◽  
Kaare M. Gautvik ◽  
Sjur Reppe ◽  
Anna Teti ◽  
...  
Keyword(s):  
Bone Turnover ◽  
Healthy Subjects ◽  
Serum Levels ◽  
Lipocalin 2 ◽  
Bone Turnover Biomarkers

scholarly journals Application of regularized regression to identify novel predictors of mortality in a cohort of hemodialysis patients

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Stanislas Werfel ◽  
Georg Lorenz ◽  
Bernhard Haller ◽  
Roman Günthner ◽  
Julia Matschkal ◽  
...  
Keyword(s):  
Statistical Learning ◽  
Cox Regression ◽  
Serum Levels ◽  
Hemodialysis Patients ◽  
Derivation Cohort ◽  
Predictors Of Mortality ◽  
Stage Renal Disease ◽  
End Stage ◽  
Study Participants ◽  
Related Mortality

AbstractCohort studies often provide a large array of data on study participants. The techniques of statistical learning can allow an efficient way to analyze large datasets in order to uncover previously unknown, clinically relevant predictors of morbidity or mortality. We applied a combination of elastic net penalized Cox regression and stability selection with the aim of identifying novel predictors of mortality in a cohort of prevalent hemodialysis patients. In our analysis we included 475 patients from the “rISk strAtification in end-stage Renal disease” (ISAR) study, who we split into derivation and confirmation cohorts. A wide array of examinations was available for study participants, resulting in over a hundred potential predictors. In the selection approach many of the well established predictors were retrieved in the derivation cohort. Additionally, the serum levels of IL-12p70 and AST were selected as mortality predictors and confirmed in the withheld subgroup. High IL-12p70 levels were specifically prognostic of infection-related mortality. In summary, we demonstrate an approach how statistical learning can be applied to a cohort study to derive novel hypotheses in a data-driven way. Our results suggest a novel role of IL-12p70 in infection-related mortality, while AST is a promising additional biomarker in patients undergoing hemodialysis.

scholarly journals The holding temperature of blood during a delay to processing can affect serum and plasma protein measurements

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Milton Ashworth ◽  
Benjamin Small ◽  
Lucy Oldfield ◽  
Anthony Evans ◽  
William Greenhalf ◽  
...  
Keyword(s):  
Healthy Subjects ◽  
Room Temperature ◽  
Serum Levels ◽  
Data Interpretation ◽  
Blood Samples ◽  
Specific Manner ◽  
Associated Proteins ◽  
Holding Temperature ◽  
Pai 1 ◽  
Meaningful Data

AbstractAccurate blood-borne biomarkers are sought for diagnosis, prognosis and treatment stratification. Consistent handling of blood is essential for meaningful data interpretation, however, delays during processing are occasionally unavoidable. We investigated the effects of immediately placing blood samples on ice versus room temperature for 1 h (reference protocol), and holding samples on ice versus room temperature during a 3 h delay to processing. Using Luminex multi-plex assays to assess cytokines (n = 29) and diabetes-associated proteins (n = 15) in healthy subjects, we observed that placing blood samples immediately on ice decreased the serum levels of several cytokines, including PAI-1, MIP1-β, IL-9, RANTES and IL-8. During a delay to processing, some analytes, e.g. leptin and insulin, showed little change in serum or plasma values. However, for approximately half of the analytes studied, a delay, regardless of the holding temperature, altered the measured levels compared to the reference protocol. Effects differed between serum and plasma and for some analytes the direction of change in level varied across individuals. The optimal holding temperature for samples during a delay was analyte-specific. In conclusion, deviations from protocol can lead to significant changes in blood analyte levels. Where possible, protocols for blood handling should be pre-determined in an analyte-specific manner.

scholarly journals Trace elements in patients with aortic valve sclerosis

2021 ◽  
Vol 15 ◽  
pp. 175394472098598
Author(s):  
Hataw Al-Taesh ◽  
Abuzer Çelekli ◽  
Murat Sucu ◽  
Seyithan Taysi
Keyword(s):  
Trace Elements ◽  
Aortic Valve ◽  
Inductively Coupled Plasma ◽  
Healthy Subjects ◽  
Serum Zinc ◽  
Serum Levels ◽  
Control Group ◽  
Aortic Valve Sclerosis ◽  
Inductively Coupled ◽  
Significant Difference

Background: Aortic valve sclerosis (AVSc) is defined as the thickening and calcification of aortic valve cusps, in the absence of obstruction of ventricular outflow. AVSc is linked with a clear imbalance in some trace elements. Aims: The objective of this study was to investigate the relationship between AVSc and serum levels of iron (Fe), zinc (Zn), selenium (Se), and copper (Cu). Additionally, this research aimed to explore the clinical significance of human serum zinc, selenium, copper, and iron concentrations as a potential new biomarker for AVSc patients and to clarify the pathophysiological role in individuals at risk of developing AVSc. Patients and methods: The study included 40 subjects with AVSc (25% male and 75% female) who were compared with a healthy control group with the same gender ratio. AVSc was based on comprehensive echocardiographic assessments. Blood samples were taken and Zn and Cu concentrations were determined through the use of atomic absorption spectroscopy. Se was measured using an inductively coupled plasma mass spectrometry device and Fe was measured using a Beckman Coulter instrument. Results: There was a significant difference in the prevalence of diabetes, blood pressure levels, and body mass index between the patients and the healthy subjects ( p < 0.05). The differences between the serum Fe, Se, and Cu levels of the AVSc patients and the healthy subjects ( p > 0.05) were recorded. The serum Zn of AVSc patients when compared was significantly lower compared with that of the control group ( p < 0.01). Conclusion: Patients with AVSc had an imbalance in some of the trace elements in their blood. The patient group’s valves had higher serum Cu levels and lower serum Se, Zn, and Fe concentrations compared with the healthy group’s valves. In the valve patients as compared, AVSc had a high prevalence of obesity, hypertension, and diabetes.

scholarly journals Could KL-6 levels in COVID-19 help to predict lung disease?

2020 ◽  
Vol 21 (1) ◽  
Author(s):  
AN Frix ◽  
L. Schoneveld ◽  
A. Ladang ◽  
M. Henket ◽  
B. Duysinx ◽  
...  
Keyword(s):  
Lung Disease ◽  
Healthy Subjects ◽  
Lung Diseases ◽  
Therapeutic Response ◽  
Healing Process ◽  
Response Prediction ◽  
Serum Levels ◽  
Interstitial Lung Diseases ◽  
Type Ii Pneumocytes ◽  
Epithelial Lesions

Abstract Background Coronavirus disease COVID-19 has become a public health emergency of international concern. Together with the quest for an effective treatment, the question of the post-infectious evolution of affected patients in healing process remains uncertain. Krebs von den Lungen 6 (KL-6) is a high molecular weight mucin-like glycoprotein produced by type II pneumocytes and bronchial epithelial cells. Its production is raised during epithelial lesions and cellular regeneration. In COVID-19 infection, KL-6 serum levels could therefore be of interest for diagnosis, prognosis and therapeutic response evaluation. Materials and methods Our study retrospectively compared KL-6 levels between a cohort of 83 COVID-19 infected patients and two other groups: healthy subjects (n = 70) on one hand, and a heterogenous group of patients suffering from interstitial lung diseases (n = 31; composed of 16 IPF, 4 sarcoidosis, 11 others) on the other hand. Demographical, clinical and laboratory indexes were collected. Our study aims to compare KL-6 levels between a COVID-19 population and healthy subjects or patients suffering from interstitial lung diseases (ILDs). Ultimately, we ought to determine whether KL-6 could be a marker of disease severity and bad prognosis. Results Our results showed that serum KL-6 levels in COVID-19 patients were increased compared to healthy subjects, but to a lesser extent than in patients suffering from ILD. Increased levels of KL-6 in COVID-19 patients were associated with a more severe lung disease. Discussion and conclusion Our results suggest that KL-6 could be a good biomarker to assess ILD severity in COVID-19 infection. Concerning the therapeutic response prediction, more studies are necessary.

Association of food intake with serum levels of phosphorus and potassium in hemodialysis patients

2014 ◽  
Vol 47 (1) ◽  
pp. 33 ◽  
Author(s):  
Hye Jin Woo ◽  
Yeon Joo Lee ◽  
Il Hwan Oh ◽  
Chang Hwa Lee ◽  
Sang Sun Lee
Keyword(s):  
Food Intake ◽  
Serum Levels ◽  
Hemodialysis Patients ◽  
Phosphorus And Potassium

scholarly journals Effect of short-term erythropoietin therapy on insulin resistance and serum levels of leptin and neuropeptide Y in hemodialysis patients

2017 ◽  
Vol 21 (5) ◽  
pp. 724 ◽  
Author(s):  
OsamaA Khamis ◽  
HamedM Osman ◽  
MohamedS Elfeky ◽  
AmaniM El Amin Ali ◽  
MostafaY Abdelwahed
Keyword(s):  
Insulin Resistance ◽  
Neuropeptide Y ◽  
Serum Levels ◽  
Hemodialysis Patients ◽  
Short Term

scholarly journals Angiotensin-Converting Enzyme (ACE) D Allele as a Risk Factor for Increase Serum Interleukin-6 and Interleukin-8 in Psoriasis Patients

2018 ◽  
Vol 6 (5) ◽  
pp. 772-776 ◽  
Author(s):  
Ahmed I. Abd Elneam ◽  
Mohammed Saleh Al-Dhubaibi ◽  
Ali Ismaiel Ali Abd Alrheam
Keyword(s):  
Risk Factor ◽  
Healthy Subjects ◽  
Serum Levels ◽  
Converting Enzyme ◽  
Inflammatory Skin Disease ◽  
Ace Polymorphism ◽  
Ace Gene ◽  
Ace Gene Polymorphism ◽  
The Relationship

BACKGROUND: Psoriasis is a chronic, recurrent inflammatory skin disease. It is characterised by autoimmune, environmental factors and complex genetic disorder.AIM: To explore the role of IL-6, IL-8, and ACE I/D polymorphism in the pathogenesis of Psoriasis and investigation of the relationship between ACE polymorphism and occurrence of psoriasis.PATIENTS AND METHODS: In this study, we took 73 psoriasis patients and 47 healthy patients as a control. These two groups subjected to analysis for ACE gene I/D polymorphism by PCR and biochemical methods.RESULTS: The serum levels of ACE, IL-8 and IL-6 were statistically significantly higher in psoriasis patients compared to healthy subjects (P < 0.001). ID and DD polymorphism were more common in psoriasis patients than healthy subjects. Also, D allele was significantly over-represented in patients compared to controls (52.7% Vs 35.1%).CONCLUSION: ACE gene polymorphism might grant susceptibility to develop psoriasis.

Sign in / Sign up

Export Citation Format

Share Document