Effects of a commercial blend of essential oils and monensin in a high-grain diet containing wheat distillers’ grains on in vitro fermentation

Li, Y. L., Li, C., Beauchemin, K. A. and Yang, W. Z. 2013. Effects of a commercial blend of essential oils and monensin in a high-grain diet containing wheat distillers’ grains on in vitro fermentation. Can. J. Anim. Sci. 93: 387–398. Our objective was to evaluate in vitro effects of a commercial blend of essential oils (BEO) versus monensin (MON) on fermentation of a high-grain diet containing wheat distillers’ dried grains with solubles (DDGS). Two experiments were conducted. The first experiment was designed as a short-term batch culture to determine the optimum dose of BEO (0, 45, 90 or 180 mg kg−1DM) on fermentation variables. The second experiment was a completely randomized block design with a 2×2 factorial arrangement of treatments using the rumen simulation technique. A high-grain dietary substrate containing wheat DDGS was supplemented with 0 or 28 mg MON kg−1DM combined with 0 and 90 mg BEO kg−1DM. Monensin did not affect the total volatile fatty acid (VFA) concentration or the molar proportions of individual VFA, but reduced CH4production (mL L−1gas; P=0.01) and disappearance of DM (P=0.04). Supplementation of BEO did not affect VFA concentration, but decreased (P=0.01) the molar proportion of acetate and reduced (P=0.02) the acetate to propionate concentration ratio and CH4production (mL g−1digested organic matter). The BEO increased (P=0.01) disappearance of neutral detergent fiber without affecting disappearance of other nutrients. These results indicate that supplementation of high-grain dietary substrate containing wheat DDGS with BEO improved fermentation pattern by increasing propionate concentration, reducing CH4production, and increasing fiber digestibility. This commercial BEO could be a potential substitute for MON for beef cattle fed high-grain diets containing DDGS.

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Characteristics of Ini Vitro Fermentative Digestibility of Odot Grass (Pennisetum Purpureum Cv. Mott) At Different Planting Spacing and Defoliation Age

International Journal of Scientific Research in Science Engineering and Technology ◽

10.32628/ijsrset207225 ◽

2020 ◽

pp. 368-376

Author(s):

La Malesi ◽

Takdir Saili ◽

Ali Bain ◽

Tresjia C Rakian

Keyword(s):

Block Design ◽

Animal Husbandry ◽

Study Data ◽

Nutrient Digestibility ◽

Pennisetum Purpureum ◽

In Vitro Digestibility ◽

In Vitro Fermentation ◽

Randomized Block Design ◽

Ph Level

This study aimed to evaluate the characteristics of in vitro fermentative digestibility of odot grass planted at different spacing and defoliation ages-days after planting (DAP). The study was conducted in the Field Laboratory of the Faculty of Animal Science of the Halu Oleo University and the Laboratory of Nutrition for Dairy Sciences of the Faculty of Animal Husbandry, IPB University. The study was designed by using factorial randomized block design (3x3). The first factor was planting spacing (60 cm x 90 cm, 75 cm x 90 cm and 90 cm x 90 cm), and the second factor was defoliation age (60 DAP, 90 DAP, and 120 DAP), consisted of 4 groups and there were 36 treatment combinations. The grouping was based on the slope of the land. The study data were analyzed by using analysis of variance using SPSS 21 and if the treatment had a significant effect, a different test between treatments was tested by using the Duncan’s Multiple Range Test (DMRT) test. The experimental results showed that the interaction between planting spacing and defoliation age did not affect the characteristics of fermentation and nutrient digestibility in vitro. However, the in vitro fermentation characteristics were affected significantly (p<0.05) by defoliation age, but were not affected by planting spacing whereas the pH level was not affected by spacing and defoliation age. Further tests of the differences between treatments showed that the planting spacing treatment was not significantly different (p>0.05), whereas among the defoliation age treatments were significantly different (p<0.05). The conclusion of this study was the treatment of planting spacing and defoliation age did not affect digestibility in vitro fermentative of grass odot. The treatment of defoliation age independently influenced the in vitro digestibility of odot grass.

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Degradability, in vitro fermentation parameters and kinetic degradation of diets with increasing levels of forage and chitosan

Translational Animal Science ◽

10.1093/tas/txab086 ◽

2021 ◽

Author(s):

Amanna Gonzaga Jacaúna ◽

Rafael Henrique de Tonissi e Buschinelli de Goes ◽

Leonardo de Oliveira Seno ◽

Luis Carlos Vinhas Ítavo ◽

Jefferson Rodrigues Gandra ◽

...

Keyword(s):

Degradation Kinetics ◽

Block Design ◽

Ammonia Concentration ◽

Gas Production ◽

Ruminal Fermentation ◽

In Vitro Fermentation ◽

Randomized Block Design ◽

Fermentation Parameters ◽

Potential Use

Abstract Chitosan is the second most important natural biopolymer in the world, extracted from crustaceans, shrimps, and crabs; and can modulate rumen fermentation. Our hypothesis is that the addition of chitosan alters the fermentation patterns of different diets for ruminants. This study aimed to evaluate the effects of different levels of chitosan and forage on in vitro dry degradation kinetics and fermentation in a gas production system. The chitosan levels (0, 1625, 3500 or 7500 mg/kg of DM) were arranged in a completely randomized block design, and for in vitro ruminal fermentation assay we used a split splot arrangement. Into the incubator, all chitosan levels were distributed in the four jars, and the forage levels varying on 100, 65, 50, 35 and 20 on DM basis. Chitosan and roughage levels interaction effect (P≤0.05) on IVDMD; IVOMD. IVDCP and IVDNDF. Chitosan negatively affected IVDMD in all roughage levels evaluated. The pH and ammonia concentration present effect only for roughage levels and incubation hours. The chitosan didn’t change (P=0.3631) the total short-chain fatty acid concentration (overall mean = 21.19 mmol/L) and the C2:C3 ratio (overall mean = 5.85). The IVDCP showed the same decreasing quadratic behavior (P<0.0001). The increasing chitosan addition increases (P<0.0001) the gas production and decreases the (P<0.0001) the lag time (parameter C) of diets with greater concentrate participation, characterizing greater efficiency in the degradability of the diet, confirming its potential use in diets for ruminants. Chitosan changes in vitro dry degradation kinetics and fermentation at the minimum dose of 1722 mg/kg DM for all diets. The roughage level influenced the in vitro nutrients degradability and cumulative gas production.

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Chemical composition and fatty acid profile of BRS Capiaçu ensiled at different regrowth ages

Semina Ciências Agrárias ◽

10.5433/1679-0359.2021v42n3supl1p1981 ◽

2021 ◽

pp. 1981-2004

Author(s):

Fernando César Ferraz Lopes ◽

◽

Mirton José Frota Morenz ◽

Francisco José da Silva Lédo ◽

Jailton da Costa Carneiro ◽

...

Keyword(s):

Fatty Acid ◽

Chemical Composition ◽

Block Design ◽

Random Effect ◽

Neutral Detergent Fiber ◽

Elephant Grass ◽

Randomized Block Design ◽

Quadratic Effects ◽

Chopped Grass

This study aimed to evaluate the chemical composition and fatty acid (FA) profile of chopped forage and silage of BRS Capiaçu elephant grass at four regrowth ages: 50, 70, 90 and 110 days. A randomized block design with five replications was used. The ensiling was carried out manually in experimental silos without wilting using no additives or bacterial inoculants. The results were analyzed using mixed models (P < 0.05). The model included treatment (regrowth age) as a fixed effect and block as a random effect. Linear and quadratic effects of the treatments were analyzed using orthogonal contrasts. There were linear increases in the dry matter (DM, g kg-1) and lignin (g kg-1 DM) contents and linear reductions in the in vitro DM digestibility (g kg-1) of chopped grass and silage as a function of regrowth age (P < 0.001). Quadratic effects (P ≤ 0.01) were observed for the chopped grass contents (g kg-1 DM) of crude protein (CP), ether extract (EE) and neutral detergent fiber (NDF) as a function of regrowth age. There were linear decreases (P < 0.0001) in the CP content (g kg-1 DM) and pH and linear increases (P < 0.001) in the EE and NDF contents (g kg-1 DM) in the silage as a function of regrowth age. There were linear decreases (P < 0.01) in the chopped grass contents and linear increases (P < 0.05) in the silage contents of total FAs, linoleic and α-linolenic acids (g kg-1 DM) asa function of regrowth age. BRS Capiaçu elephant grass must be harvested at up to 70 days of regrowth to obtain forage with good nutritional value and the highest levels of linoleic and α-linolenic acids (g kg-1 DM). To produce silages with adequate pH values and the highest levels of linoleic and α-linolenic acids (g kg-1 DM), BRS Capiaçu must be harvested between 90 and 110 days of regrowth.

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Agronomic characteristics of sorghum genotypes and nutritional values of silage

Acta Scientiarum Animal Sciences ◽

10.4025/actascianimsci.v38i2.29567 ◽

2016 ◽

Vol 38 (2) ◽

pp. 127 ◽

Cited By ~ 3

Author(s):

Renê Ferreira Costa ◽

Daniel Ananias de Assis Pires ◽

Marielly Maria Almeida Moura ◽

Eleuza Clarete Junqueira de Sales ◽

José Avelino Santos Rodrigues ◽

...

Keyword(s):

Block Design ◽

Neutral Detergent Fiber ◽

Nutritional Values ◽

Agronomic Characteristics ◽

Dry Matter Digestibility ◽

Randomized Block Design ◽

Nutritional Characteristics ◽

Silage Production ◽

Sorghum Genotypes

The objective was to assess agronomic characteristics of sorghum genotypes and nutritional values of produced silage. A total of 15 sorghum genotypes were used. Planting was carried out through a randomized-block design with three replications per genotype, totaling forty-five (45) plots. Agronomic and nutritional characteristics, besides the quality of the silage, were assessed. There were differences between genotypes for all characteristics analyzed, with the exception of levels of acid detergent unavailable nitrogen (ADUN), acid detergent unavailable protein (ADUP) and water activity (aw). The assessment of the parameters, except for genotypes 1016013, 1016025, 1016037, 1016039, Volumax and BRS 610, which presented protein level below 7%, showed that the other genotypes can be used for silage production, since they have a good profile of fermentation and in vitro dry matter digestibility; however, genotype SF15 is the most favorable one for silage production due to its little participation in the neutral detergent fiber fraction.

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251 Evaluation of Condensed Algal Residue Solubles as an Ingredient in Cattle Finishing Diets

Journal of Animal Science ◽

10.1093/jas/skaa054.239 ◽

2020 ◽

Vol 98 (Supplement_3) ◽

pp. 137-138

Author(s):

Calvin Gibbons ◽

Andrea K Watson ◽

Galen E Erickson ◽

Bradley M Boyd ◽

Levi J McPhillips ◽

...

Keyword(s):

Great Plains ◽

Fixed Effects ◽

Block Design ◽

Random Effect ◽

Distillers Grains ◽

Experimental Unit ◽

Omega 3 ◽

Finishing Diets ◽

Randomized Block Design ◽

Algae Oil

Abstract Algae oil production for Omega-3 fatty acid supplementation yields a byproduct called Condensed Algal Residue Solubles (CARS; 25.4% DM, 19.3% CP, 8.3% Fat, 9.96% Na on DM basis), de-oiled algae cells with residual fermentation substrates. This study evaluated the use of CARS in feedlot finishing diets. Crossbreed steers, (n=480) were blocked and stratified by initial body weight (BW) into 4 blocks, and assigned randomly to treatments. Treatments were designed as a 2 x 3 factorial with 3 inclusions of CARS (0, 2.5, 5% of diet DM) and 2 different base diets representing Northern and Southern Great Plains diets. The Southern diets contained steam flaked corn and dry distillers grains while the Northern diets had dry rolled and high moisture corn with wet distillers grains. CARS replaced corn in both diets. All blocks were harvested after 148 days on feed. Performance data were analyzed as a randomized block design with CARS inclusion, base diet, and interactions as fixed effects, BW block as a random effect and pen (n=48) as the experimental unit. Orthogonal contrasts were used to test linear and quadratic effects of CARS inclusion. There were no significant interactions between CARS inclusion and diet type (P ≥ 0.49). Main effects of CARS indicated positive quadratic responses for carcass adjusted ADG, G:F, 12th rib back fat, yield grade (P < 0.01; increasing to 2.5% inclusion, decreasing at 5%) and hot carcass weight was both linear and quadratic (P ≤ 0.01 and P ≥ 0.06 respectively; 969, 977, 935 as CARS increased). Linear decrease in DMI, final adjusted BW and ribeye area (P ≤ 0.01) as CARS increased. Cattle fed the Southern diets had greater ADG and G:F compared to Northern diets (P < 0.01). Including 2.5% CARS in the diet improved feed efficiency in both Northern and Southern based feedlot diets.

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332 Effect of Chemical and Biological Preservatives on in vitro Fermentation and Methane Production of Ensiled and Aerobically Exposed Wet Brewer’s Grains

Journal of Animal Science ◽

10.1093/jas/skab235.330 ◽

2021 ◽

Vol 99 (Supplement_3) ◽

pp. 182-182

Author(s):

Marjorie A Killerby ◽

Diego Zamudio ◽

Kaycee Ames ◽

Darren D Henry ◽

Thomas Schwartz ◽

...

Keyword(s):

Methane Production ◽

Production System ◽

Methane Concentration ◽

Block Design ◽

Gas Production ◽

Sodium Lignosulfonate ◽

In Vitro Fermentation ◽

Randomized Complete Block Design ◽

System Data

Abstract This study evaluated the effects of preservatives on the in vitro fermentation measures of wet brewer’s grain (WBG) silage at different stages of storage. Treatments (TRT) were sodium lignosulfonate at 1% (NaL1) and 2% (NaL2; w/w of fresh WBG), propionic acid (PRP; 0.5% w/w of fresh WBG), a combination inoculant (INO; Lactococcus lactis and Lactobacillus buchneri each at 4.9 log cfu/fresh WBG g), and untreated WBG (CON). WBG (Fresh) were packed into 8.8 L mini-silos and stored for 60 d at 21°C (Ensiled), then they were opened and aerobically exposed for 10d (AES). Samples from each stage of storage (STG; Fresh, Ensiled and AES) were analyzed for in vitro ruminal digestibility (24 h).Gas kinetics were recorded using the Ankom RF Gas Production System. Data were analyzed as a randomized complete block design (5 blocks) with a 5 (TRT) × 3 (STG) factorial arrangement. Apparent in vitro DM digestibility (DMD) decreased across STG, (51.5, 47.2 and 40.9 for Fresh, Ensiled and AES, respectively) and increased for NaL1, NaL2 and PRP (~47.8) vs. CON (43.0 ± 2.12%). PRP increased apparent in vitro OM digestibility (OMD) when Ensiled (54.5) and NaL2 increased it for AES (47.1) vs CON (46.3 and 39.9 ± 1.73%, respectively). The asymptotic maximal (M) and rate (k) of gas production decreased across STG (214.6, 181.5, 155.1 and 14.6, 12.6, and 9.8, for Fresh, Ensiled and AES, respectively). PRP increased (200.0) and NaL1 decreased (169.3) M vs. CON (183.9± 7.81ml/incubated DM g), while NaL1 and NaL2 (~11.4) decreased k vs. CON (13.4 ± 0.85%/h). Methane concentration and yield were higher in Fresh vs. other STG (0.94 vs. ~0.84 ± 0.07mM and 0.27 vs. ~0.23 ± 0.03mmol/g fermented OM). Spoilage of WBG decreases fermentability and methane production while PRP and NaL improve digestibility with the former also increasing M and k.

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Nutritional value of sorghum silages

Acta Scientiarum Animal Sciences ◽

10.4025/actascianimsci.v39i2.32677 ◽

2017 ◽

Vol 39 (2) ◽

pp. 137

Author(s):

Marielly Maria Almeida Moura ◽

Daniel Ananias de Assis Pires ◽

Renê Ferreira Costa ◽

Daniella Cangussú Tolentino ◽

João Paulo Sampaio Rigueira ◽

...

Keyword(s):

Crude Protein ◽

Block Design ◽

Significance Level ◽

Dry Matter Digestibility ◽

Randomized Block Design ◽

Nutritional Characteristics ◽

High Participation ◽

Sorghum Genotypes

This study aimed to evaluate the nutritional characteristics and quality of silages of five sorghum genotypes, namely: Volumax, AG2005E, Qualimax, BRS610 and AG2501. The study was conducted at the Experimental Farm Unimontes, in the municipality of Janaúba, state of Minas Gerais. The treatments were distributed in a randomized block design with four replicates. Data were tested by analysis of variance andmeans were compared by Scott-Knott test at a significance level of 5%. The pH values ranged from 3.93 and 4.10. Genotypes differed in all studied nutritional characteristics (p < 0.05). For the acid detergent fiber, differences were detected between the genotypes, AG2501 (35.66%), Volumax (34.89%), AG2005E (34.53%), Qualimax (32.39%) and BRS610 (33.60%). The high participation of lignin was verifiedin all genotypes except for Volumax (4.14%). All silages were within the recommended range 50-65% for in vitro dry matter digestibility and classified as having good quality, however only the AG2005E genotype met nitrogen requirements for microbial fermentation, with 7.06% crude protein.

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Optimation of In vitro Lateral Shoots Multiplication of Papaya (Carica papaya L.) “Callina” with BAP and NAA

Journal of Tropical Crop Science ◽

10.29244/jtcs.4.3.102-107 ◽

2017 ◽

Vol 4 (3) ◽

pp. 102-107

Author(s):

Darda Efendi ◽

Mirza R Putra

Keyword(s):

Carica Papaya ◽

Clonal Propagation ◽

Block Design ◽

Shoot Multiplication ◽

Plant Materials ◽

Randomized Block Design ◽

Lateral Shoots ◽

Shoot Production ◽

Set Up

Papaya is a popular fruit and is grown commercially in many subtropical and tropical countries. Papayas are generally grown from seeds; therefore the offsprings are not true-to-type and could come in three sexes, female, male, and hermaphrodite. Clonal propagation is required to obtain to grow true-to-type hermaphrodite papayas. In this research, we developed an in vitro protocol for shoot multiplication from lateral shoots from in vitro germinated papaya seedlings. The in vitro propagated plant materials could potentially be used as a source of papaya micro cuttings, or as scion for papaya grafting. The experiment was set up as a factorial experiment with NAA at 0, 0.1 and 0.5 mg.L-1, and BAP at 0, 0.1, 0.5, and 1.0 mg.L-1 in a completely randomized block design. BAP interacted with NAA in affecting the shoot production per explant. The optimum BAP and NAA concentration to produce lateral shoots was 0.54 mg. L-1 and 0.1 mg.L-1, respectively. Media without NAA reduced the number of lateral shoots and number of leaf per explant at any BAP concentration.Keywords: hermaphrodite, seeds, true to type, clonal propagation, micro cuttings.

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Uji Efektivitas Rizobakteria Dalam Menghambat Perkembangan Penyakit Hawar Pelepah Daun (Rhizoctonia solani Kuhn.) Pada Padi Secara In Vitro

EnviroScienteae ◽

10.20527/es.v16i1.8999 ◽

2020 ◽

Vol 16 (1) ◽

pp. 44

Author(s):

Hanisa Desy Ariani ◽

Noor Aidawati ◽

Dewi Arika Adriani

Keyword(s):

Pseudomonas Aeruginosa ◽

Rhizoctonia Solani ◽

Plant Protection ◽

Block Design ◽

Sheath Blight ◽

In Vitro Test ◽

Sheath Blight Disease ◽

Randomized Block Design ◽

Blight Disease

One of the causes of the declining productivity of rice is sheath blight disease caused by the mold Rhizoctonia solani Kuhn. Control of sheath blight disease that is often done by the farmers is by using chemical pesticides (fungicides), which caused environmental problems. One way to reduce the use of pesticides is to biological control by using antagonist bacteria. This study aimed at in vitro test of rhizobacteria in preventing the development of sheath blight disease in rice plants. This research was conducted in the Phytopathology laboratory of Plant Protection Department of Faculty Agriculture, University of Lambung Mangkurat Banjarbaru from March to May 2018. The experiment used a randomized block design with three groups consisting of eight types of rhizobacteria isolates: (r1) Pseudomonas aeruginosa (Barito Kuala), (r2) Bacillus megaterium (Hulu Sungai Tengah), (r3) Azotobacter sp. (Barito Kuala), (r4) Pseudomonas sp. (Hulu Sungai Selatan), (r5) Flavobacterium sp. (Tanah Laut), (r6) Bacillus bodius (Barito Kuala), (r7) Pseudomonas aeruginosa (Hulu Sungai Selatan), (r8) Necercia sp. (Tanah Laut). The results showed that all rhizobacteria have the ability to inhibit the development of R. solani with different percentages of inhibitions. Pseudomonas aeruginosa (Barito Kuala) was the most effective rhizobacteria in inhibiting the development of R. solani.

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COCONUT MEAL INCLUSION IN HIGH CONCENTRATE SHEEP DIETS AFFECTS FEED DIGESTIBILITY AND INTAKE1

Revista Caatinga ◽

10.1590/1983-21252021v34n321rc ◽

2021 ◽

Vol 34 (3) ◽

pp. 692-701

Author(s):

PAULA FABRINY MAUÉS DA SILVA ◽

SARAH OLIVEIRA SOUSA PANTOJA ◽

FELIPE NOGUEIRA DOMINGUES ◽

ANIBAL COUTINHO DO RÊGO ◽

CRISTIAN FATURI

Keyword(s):

Soybean Meal ◽

Crude Protein ◽

Low Cost ◽

Block Design ◽

Control Diet ◽

Neutral Detergent Fiber ◽

Feed Digestibility ◽

Randomized Block Design ◽

Body Weights ◽

Coconut Meal

ABSTRACT Coconut meal can be an affordable low-cost alternative to noble ingredients such as corn and soybean meal in sheep diets. The objective of this study was to evaluate the effects of coconut meal inclusions, when used as a corn and soybean meal replacement, on the intake, digestibility, and nitrogen balance of sheep diets. Twenty-four male Santa Inês lambs with body weights (BW) of 26.6 ± 3.2 kg were used in the study. The animals were housed in metabolic cages and distributed in a completely randomized block design, with three treatments and eight replications. The animals received a total mixed ratio of 30% roughage (elephant grass) and 70% concentrate. The animals were fed on two experimental diets with 11% and 22% coconut meal inclusion and a control diet (without coconut meal). Dry matter (DM), crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF), and non-fibrous carbohydrate (NFC) intake as a percentage of BW were negatively influenced by the inclusion of coconut meal. The ether extract (EE) intake increased but did not differ between the 11% and 22% coconut meal diets. Furthermore, the digestibility of DM, CP, EE, and ADF did not differ between the two evaluated diets. The inclusion of coconut meal reduced NDF digestibility and increased that of NFC. The level of inclusion of coconut meal in the diet must be based on the EE content in the meal; keeping the EE intake below 0.16% of the BW helped to avoid limited intake of feed.

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