scholarly journals Qualitative and Quantitative Phytochemical Analysis of Centella asiatica

2018 ◽  
Vol 06 (04) ◽  
Author(s):  
Arpita Roy M ◽  
Laxmi Krishnan ◽  
Arpita Roy Roy
Keyword(s):  
Centella Asiatica ◽  
Phytochemical Analysis ◽  
Qualitative And Quantitative

QUALITATIVE AND QUANTITATIVE PHYTOCHEMICAL SCREENING AND IN VITRO ANTIOXIDANT EVALUATION OF PERGULARIA DAEMIA

2018 ◽  
Vol 24 (2) ◽  
Author(s):  
GITA MISHRA ◽  
HEMESHWER KUMAR CHANDRA ◽  
NISHA SAHU ◽  
SATENDRA KUMAR NIRALA ◽  
MONIKA BHADAURIA
Keyword(s):  
Reducing Sugars ◽  
Antioxidant Properties ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Qualitative And Quantitative ◽  
The Family ◽  
Ethanolic Extracts ◽  
Antioxidant Evaluation ◽  
In Vitro Antioxidant Activity

Pergularia daemia belongs to the family Asclepiadaceae, known to have anticancer, anti-inflammatory activity. Aim of the present study was to evaluate qualitative and quantitative phytochemical and antioxidant properties of ethanolic extracts of leaf, stem and root parts of P. daemia . Preliminary phytochemical analysis and in vitro antioxidant properties were evaluated by standard methods. The qualitative phytochemical analysis of P. daemia showed presence of flavonoids, tannins, alkaloid, phytosterol, carbohydrate, phenol, saponin, glycosides, terpenoids, steroids proteins and reducing sugars. Quantitative analysis showed polyphenol, flavonoid, flavonone, flavone and flavonol in P. daemia leaves, stem and root in considerable quantity. The in vitro antioxidant activity of P. daemia clearly demonstrated that leaf, stem and root parts have prominent antioxidant properties and was effective in scavenging free radicals.

Comparative Pharmacognostic, Physicochemical and Phytochemical Studies of Different Samples of Plumbago zeylanica L. Roots

2017 ◽  
Vol 9 (06) ◽  
Author(s):  
Neha Jain ◽  
Mohan Lal Kori
Keyword(s):  
Phytochemical Analysis ◽  
Local Market ◽  
Herbal Drugs ◽  
Disease Treatment ◽  
Organoleptic Evaluation ◽  
Plumbago Zeylanica ◽  
Qualitative And Quantitative ◽  
Crude Drugs ◽  
Phytochemical Studies

The objective of the present study is to evaluate the quality of the marketed and self collected samples of Plumbago zeylanica L. roots on the standardization parameters. This study is planned mainly to confirm changes with quality of drug. Now a day’s more demand of herbal drugs for disease treatment, lack of knowledge of proper methodology and availability are promoting the practices of adulteration and substitution. Thus, the standardization of the plant crude drugs is necessary to maintain their therapeutic efficacy. Comparative studies were carried out to evaluate the standards of P. zeylanica L. with emphasis on organoleptic evaluation, physicochemical and phytochemical analysis. Samples were procured from local market and self collected to determine the qualitative and quantitative variations. The result indicates that self collected sample showed significant results with comparison to marketed sample.

scholarly journals Pharmacognostic Evaluation of Curcuma longa L. Rhizome and Standardization of its Formulation by HPLC Using Curcumin as Marker

2018 ◽  
Vol 10 (01) ◽  
Author(s):  
Abraham A ◽  
Samuel S ◽  
Mathew L
Keyword(s):  
Cross Section ◽  
Curcuma Longa ◽  
Microscopic Analysis ◽  
Fluorescence Analysis ◽  
Phytochemical Analysis ◽  
Diagnostic Features ◽  
Starch Grains ◽  
Qualitative And Quantitative ◽  
Polyherbal Formulation ◽  
Laboratory Procedures

Objective: To evaluate Curcuma longa rhizome by pharmacognostic and phytochemical analysis and standardize one of its formulations by HPLC. The present work includes macroscopic study, microscopic analysis of cross section and powder of rhizome, fluorescence analysis, qualitative and quantitative phytochemical assay and chromatographic fingerprinting of its polyherbal formulation, Pathyashadangam kwath using curcumin as marker carried out as per standard laboratory procedures. The study helps to provide both diagnostic features for identification and preventing adulteration of Curcuma longa L and HPLC chromatogram for standardisation of its formulation. Results: Microscopic analysis revealed the presence of a broad parenchymatous cortex with abundant starch grains in the cross section, spiral vessels, starch grains having hilum towards the narrower end, fibres and cells with oleo resin in powder microscopy. HPLC analysis revealed the presence of three peaks corresponding to curcuminoids in the formulation. Conclusion: Pharmacognostic and phytochemical evaluation can be used for confirming the identity of Curcuma longa rhizome and HPLC using curcumin as marker can be used for standardisation of its formulations.

scholarly journals Phytochemical Analysis of Podospermum and Scorzonera n-Hexane Extracts and the HPLC Quantitation of Triterpenes

Molecules ◽  
2018 ◽  
Vol 23 (7) ◽  
pp. 1813 ◽  
Author(s):  
Özlem Bahadır-Acıkara ◽  
Serkan Özbilgin ◽  
Gülcin Saltan-İşcan ◽  
Stefano Dall’Acqua ◽  
Veronika Rjašková ◽  
...  
Keyword(s):  
Hplc Method ◽  
Phytochemical Analysis ◽  
Qualitative And Quantitative Analysis ◽  
Qualitative And Quantitative ◽  
Aerial Parts ◽  
Hexane Extracts ◽  
Lupeol Acetate ◽  
Anti Inflammatory

Previously tested n-hexane extracts of the Scorzonera latifolia showed promising bioactivity in vivo. Because triterpenes could account for this activity, n-hexane extracts were analyzed by HPLC to identify and quantify the triterpenes as the most abundant constituents. Other Scorzonera and Podospermum species, potentially containing triterpenic aglycones, were included in the study. An HPLC method for simultaneous determination of triterpene aglycones was therefore developed for analysis of Podospermum and Scorzonera species. n-Hexane extracts of root and aerial parts of S. latifolia, ten other Scorzonera species and two Podospermum species were studied to compare the content of triterpenes. HPLC was used for the qualitative and quantitative analysis of α-amyrin, lupeol, lupeol acetate, taraxasteryl acetate, 3-β-hydroxy-fern-7-en-6-one acetate, urs-12-en-11-one-3-acetyl, 3-β-hydroxy-fern-8-en-7-one acetate, and olean-12-en-11-one-3-acetyl. Limits of detection and quantification were determined for each compound. HPLC fingerprinting of n-hexane extracts of Podospermum and Scorzonera species revealed relatively large amounts of triterpenes in a majority of investigated taxa. Lupeol, lupeol acetate, and taraxasteryl acetate were found in a majority of the species, except S. acuminata. The presence of α-amyrin, 3β-hydroxy-fern-7-en-6-one-acetate, urs-12-en-11-one-3-acetyl, 3β-hydroxy-fern-8-en-7-one-acetate, and olean-12-en-11-one-3-acetyl was detected in varying amounts. The triterpene content could correlate with the analgesic and anti-inflammatory activity of Scorzonera, which was previously observed and Scorzonera species that have been determined to contain triterpenes in large amounts and have not yet been tested for their analgesic activity should be tested for their potential analgesic and anti-inflammatory potential. The presented HPLC method can be used for analysis of triterpene aglycones, for example dedicated to chemosystematic studies of the Scorzonerinae.

scholarly journals Qualitative and Quantitative Phytochemical Analysis of Different Extracts from Thymus algeriensis Aerial Parts

Molecules ◽  
2018 ◽  
Vol 23 (2) ◽  
pp. 463 ◽  
Author(s):  
Nassima Boutaoui ◽  
Lahcene Zaiter ◽  
Fadila Benayache ◽  
Samir Benayache ◽  
Simone Carradori ◽  
...  
Keyword(s):  
Phytochemical Analysis ◽  
Qualitative And Quantitative ◽  
Thymus Algeriensis ◽  
Aerial Parts

scholarly journals Comparative Phytochemical Analysis of Syzygium formosum (Wall.) Masam Leaf and Its Biological Activities

2021 ◽  
Vol 11 (22) ◽  
pp. 10552
Author(s):  
Khanh Hong Thi Hoang ◽  
Jaeyoon Lim ◽  
My Tuyen Thi Nguyen ◽  
Nan-Young Lee ◽  
Chang-Kyu Lee ◽  
...  
Keyword(s):  
Leaf Extract ◽  
Biological Activities ◽  
Centella Asiatica ◽  
Human Keratinocytes ◽  
Pharmaceutical Products ◽  
Biologically Active ◽  
Phytochemical Analysis ◽  
Asiatic Acid ◽  
High Concentration ◽  
Cutibacterium Acnes

Syzygium formosum (SF) leaves have long been used in traditional medicine for the treatment of skin-related diseases such as atopic dermatitis and psoriasis. To understand the underpinning pharmacological mechanism of skin relief functionality, the quantitative profile of phytochemicals in the leaf extract has been performed. Twenty phytochemical components including eight flavonoids, three phenolic acids, and nine triterpenoids were identified and quantitatively analyzed from the SF leaves. The dominant flavonoids of the SF leaves were found to be catechin and myricetin-3-O-rhamnoside. Gallic acid was the most abundant phenolic acid. Among triterpenoids, asiatic acid, corosolic acid, and betulinic acid exhibited a high concentration ranging 2.78–8.59, 3.19–3.91, and 3.58–6.14 mg/g dry leaf, respectively. The total triterpenoid contents were 14.18–19.00 mg/g dry leaf which were 3-fold higher than those in the Indonesian Centella asiatica leaves. In human keratinocytes assay, industrial scale extract of SF significantly reduced expressions of proinflammatory cytokines and cyclooxygenase-2. Furthermore, that SF extract exhibited very strong antibacterial activity on Staphylococcus aureus and Cutibacterium acnes that possibly induce serious skin problems. Our results proved that S. formosum leaf extract with high content of biologically active compounds could be a superior bioactive ingredient in cosmetic and pharmaceutical products.

scholarly journals Exploring the Ethnopharmaceutical Plants of Osing Tribe in Banyuwangi Regency: Potential Application for COVID-19 Therapy

2021 ◽  
pp. 113-120
Author(s):  
Endah Puspitasari ◽  
Alfina Eka Damayanti ◽  
Intan Nabila Sufi Zikrina ◽  
Dewi Dianasari
Keyword(s):  
Quantitative Research ◽  
Herbal Medicines ◽  
Centella Asiatica ◽  
Immunomodulatory Activity ◽  
Structured Interviews ◽  
Literature Study ◽  
Qualitative And Quantitative ◽  
Informant Consensus ◽  
Plant Exploration ◽  
Further Development

Ethnopharmacy is the study on herbs or plants that certain ethnic groups practice for treating particular illness. Scientific reporting of beneficial therapeutic plants through this study could promote further development of herbal medicines. We conducted an ethnopharmacy study at several villages of Osing tribe located in Banyuwangi, Indonesia, to identify plants that have the potential to be tested for certain bioactivity, in this case, for COVID-19 therapy. The snowball and purposive sampling methods using qualitative and quantitative research with semi-structured interviews and questionnaires were applied for this study. The parameters used were the Use Value (UV), Informant Consensus Factor (ICF), and Fidelity Level (FL). The plants used in this study were obtained and determined at Balai Konservasi Tumbuhan Kebun Raya Purwodadi, the Indonesian Institute of Science. The results were then followed by the literature study on the plants’ potential for COVID-19 therapy. Plant exploration was obtained by considering the results of UV calculation. Based on UV calculations in ethnopharmacy studies, there are several plants that are considered essential and have more efficacies. They are temulawak, turmeric, suruh, gigen-gigen, mating, anggrek merpati and pace. Three of the eight plants potentially possess immunomodulatory activity that can be used to prevent the infections of SARS-CoV-2. They are the temulawak (Curcuma xanthorrhiza), turmeric (Curcuma domestica) and gigen-gigen (Centella asiatica).

scholarly journals Qualitative and Quantitative Phytochemical Analysis of Ononis Hairy Root Cultures

2021 ◽  
Vol 11 ◽  
Author(s):  
Nóra Gampe ◽  
Zoltán Szakács ◽  
András Darcsi ◽  
Imre Boldizsár ◽  
Éva Szőke ◽  
...  
Keyword(s):  
Hairy Root ◽  
Biological Effects ◽  
Limited Range ◽  
Qualitative Description ◽  
Herbal Remedies ◽  
Phytochemical Analysis ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Qualitative And Quantitative ◽  
First Time

Hairy root cultures are genetically and biochemically stable, and they regularly possess the same or better biosynthetic capabilities for specialized (secondary) metabolite production compared to the intact plant. Ononis species are well-known herbal remedies in ethnopharmacology and rich sources of isoflavonoids. Besides isoflavones, less prevalent isoflavones and pterocarpans with valuable biological effects can be found in Ononis species as well. As these plants are only collected but not cultivated, biotechnological methods could play a role in the larger-scale extraction of Ononis isoflavonoids. Regarding this information, we aimed to establish Ononis spinosa and Ononis arvensis hairy root cultures (HRCs) and analyze the isoflavonoid profile of hairy root cultures qualitatively and quantitatively, in order to define their capacity to produce biologically valuable isoflavonoids. During the qualitative description, beside isoflavonoids, two new phenolic lactones, namely, bulatlactone 2″-O-β-D-glucoside and ononilactone, were isolated, and their structures were characterized for the first time. Altogether, 29 compounds were identified by the means of UPLC-Orbitrap-MS/MS. Based on UHPLC-UV-DAD measurements, the isoflavonoid spectrum of the Ononis HRCs differed markedly from wild-grown samples, as they produce a limited range of the scaffolds. The most abundant compounds in the HRCs were medicarpin glucoside and sativanone glucoside. The overall isoflavonoid production of the cultures was comparable to wild-grown O. arvensis and approximately twice as high as in wild-grown O. spinosa samples. As the overall content of wild-grown samples include more isoflavonoid derivatives, the HRCs contain structurally less divergent isoflavonoids but in higher quantity.

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