scholarly journals <i>Low Dose</i> Irbesartan Has a Renoprotective Effect as High Dose Ramipril in Diabetic Rats Treated with Insulin

2015 ◽  
Vol 05 (11) ◽  
pp. 149-161
Author(s):  
Abdulmonim A. Alqasim ◽  
Sami H. Hammadi ◽  
Ghazi A. Bamagous ◽  
Essam Eldin M. Noureldin ◽  
Hussam H. Madi
Keyword(s):  
Low Dose ◽  
Diabetic Rats ◽  
High Dose ◽  
Renoprotective Effect

Effect of atorvastatin on the angiogenic responsiveness of coronary endothelial cells in normal and streptozotocin (STZ) induced diabetic rats

2014 ◽  
Vol 92 (4) ◽  
pp. 338-349 ◽  
Author(s):  
Kiranj K. Chaudagar ◽  
Anita A. Mehta
Keyword(s):  
Endothelial Cells ◽  
Low Dose ◽  
Ischemia Reperfusion ◽  
Late Phase ◽  
Diabetic Rats ◽  
Lipid Lowering ◽  
Diabetic Rat ◽  
High Dose ◽  
Atorvastatin Treatment ◽  
Coronary Endothelial Cells

Atorvastatin, a lipid lowering agent, possesses various pleiotropic vasculoprotective effects, but its role in coronary angiogenesis is still controversial. Our objective was to study the effects of atorvastatin on the angiogenic responsiveness of coronary endothelial cells (cEC) from normal and diabetic rats. Male Wistar rats were distributed among 9 groups; (i) normal rats, (ii) 30 day diabetic rats, (iii) 60 day diabetic rats, (iv) normal rats administered a low dose of atorvastatin (1 mg/kg body mass, per oral (p.o.), for 15 days); (v) 30 day diabetic rats administered a low dose of atorvastatin; (vi) 60 day diabetic rats administered a low dose of atorvastatin; (vii) normal rats administered a high dose of atorvastatin (5 mg/kg, p.o., for 15 days); (viii) 30 day diabetic rats administered a high dose of atorvastatin; (ix) 60 day diabetic rats administered a high dose of atorvastatin. Each group was further divided into 2 subgroups, (i) sham ischemia–reperfusion and (ii) rats hearts that underwent ischemia–reperfusion. Angiogenic responsiveness the and nitric oxide (NO) releasing properties of the subgroups of cECs were studied using a chorioallantoic membrane assay and the Griess method, respectively. Atorvastatin treatment significantly increased VEGF-induced angiogenic responsiveness and the NO-releasing properties of cECs from all of the subgroups, compared with their respective non-treated subgroups except for the late-phase diabetic rat hearts that underwent ischemia–reperfusion, and the high dose of atorvastatin treatment groups. These effects of atorvastatin were significantly inhibited by pretreatment of cECs with l-NAME, wortmannin, and chelerythrine. Thus, treatment with a low dose of atorvastatin improves the angiogenic responsiveness of the cECs from normal and diabetic rats, in the presence of VEGF, via activation of eNOS–NO release.

Effects of Scutellaria barbata extracts on serum glycolipid hormones and pancreatic-tissue structure in type 2 diabetic rats

2019 ◽  
Author(s):  
Ruile Song ◽  
Chunyang Tian ◽  
Miao Xian ◽  
Tang LI ◽  
Zhengli Chen ◽  
...  
Keyword(s):  
Low Dose ◽  
Serum Levels ◽  
Pancreatic Tissue ◽  
Diabetic Rats ◽  
Tissue Structure ◽  
High Dose ◽  
Model Group ◽  
Citrate Buffer ◽  
Scutellaria Barbata

The study describes the effect of Chinese herbal medicine extracts from Scutellaria barbata on serum glycolipid hormones and pancreatic-tissue structure in type 2 diabetes mellitus (T2DM) rats. Healthy Wistar rats (n = 150) were divided randomly into two groups: healthy (30) and model (120). The healthy group was fed normally for 30d and injected citrate buffer on day 31. The model group was fed high-fat and high-sugar feed for 30d and injected STZ on day 31. On day 38 the model group was divided randomly into four groups: model group, low-, medium-, and high-dose S. barbata (n = 30 in each). 5, 10, 20, and 30dafter treatment, serum levels of FGB, INS, CORT, TG, TC, HDL-C, and LDL-C in all groups were measured and pancreatic-tissue sections were stained with hematoxylin–eosin and aldehyde fuchsine to observe the tissue structure. The results showed significantly decreased levels of FGB, INS, CORT, and LDL-C (P less than 0.01) and increased level of HDL-C (P greator than 0.05).In addition, the damaged structure of diabetic pancreatic tissue has been partially restored in the low-dose S. barbata group. These results show that low-dose S. barbata extract treatment is effective in treating T2DM.

scholarly journals Melatonin and Magnesium Restores Neurohistopathological Changes in the Hippocampus of Streptozotocin-Induced Diabetic Rats

2020 ◽  
Vol 11 (2) ◽  
pp. 71-79
Author(s):  
Elvis Godam ◽  
◽  
Wilson Hamman ◽  
Sunday Oladele ◽  
Modupeola Samaila ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Low Dose ◽  
Organ Damage ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
High Dose ◽  
Global Epidemic ◽  
Management Measures ◽  
Patients With Diabetes ◽  
Ca1 Area

Diabetic encephalopathy and its associated end organ damage have become a major global epidemic in many patients with diabetes mellitus. These diseased conditions are complex and poorly understood, therefore the need to seek for alternative management measures to attenuate the complications associated with it. The aim of this study was to evaluate the effects of co-administration of melatonin and magnesium on the cytoarchitecture of the hippocampus of streptozotocin (STZ) induced diabetic rats. STZ was used to induce type 1 diabetes mellitus. Fifty four rats: Forty eight diabetic and six normoglycaemic rats distributed in nine groups as follow; normal control, diabetic control (DC), melatonin low dose (MLD, 10 mg/kg), magnesium low dose (MgLD, 240 mg/kg), melatonin and magnesium combined dose (MMgLD, 10 mg/kg+240 mg/kg, respectively), melatonin high dose (MHD, 20 mg/kg), magnesium high dose (MgHD, 480 mg/kg), melatonin and magnesium high dose (MMgHD, 20 mg/kg+480 mg/kg, respectively) and insulin (IN, 500 mg/kg). Melatonin and insulin were administered through intraperitoneal injections while magnesium was orally. The control groups were given placebo and all treatments were for twenty-one days. Results showed distortion of hippocampal CA1 area in the diabetic control, MgLD, MgHD, MMgHD and IN groups. MLD, MHD and MMgLD groups showed organized structures of hippocampus CA1 area with no cellular distortions, while there were less positive GFAP in the MLD, MHD and MMgLD groups. The DC, MgLD. MgHD, MMgHD and IN groups showed strong GFAP reactivity. In conclusion, MLD, MHD and MMgLD increased neuroprotection of hippocampal neurocytes

Intraperitoneal insulin is more potent than subcutaneous insulin at restoring hepatic insulin-like growth factor-I mRNA levels in the diabetic rat: a functional role for the portal vascular link

1992 ◽  
Vol 9 (3) ◽  
pp. 257-263 ◽  
Author(s):  
D. L. Russell-Jones ◽  
M. Rattray ◽  
V. J. Wilson ◽  
R. H. Jones ◽  
P. H. Sönksen ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Low Dose ◽  
Mrna Level ◽  
Diabetic Rats ◽  
Hormonal Control ◽  
Diabetic Rat ◽  
High Dose ◽  
Mrna Levels ◽  
Igf I ◽  
Intraperitoneal Insulin

ABSTRACT There is evidence that the hormonal control of hepatic IGF-I production is mediated by GH and insulin. To elucidate the role of these hormones further we administered s.c. or i.p. insulin (at 2·5 and 5·0 IU/day) and/or GH (0·8 IU/day) to rats made diabetic with streptozotocin 16 days previously. Hepatic IGF-I production was then assessed by quantifying hepatic IGF-I mRNA levels by autoradiography of Northern blots. Diabetes resulted in a fivefold reduction in hepatic IGF-I mRNA levels (optical density (OD) of the 0·7–1·1 kb band: controls, 1·3±0·09; diabetics, 0·28±0·08; P<0·01), which was not significantly changed by treatment with s.c. insulin (OD: low dose, 0·55±0·05; high dose, 0·58±0·05) or low dose i.p. insulin (OD: 0·40±0·03). High dose i.p. insulin enhanced hepatic IGF-I mRNA levels (OD: 0·93±0·23) compared with diabetic rats (P<0·01) and those given high dose s.c. insulin (P<0·04), despite the blood glucose values being similar in the treated groups (i.p., 4·72±0·29 mmol/l; s.c., 3·32±0·03 mmol/l). Administration of GH alone partially restored the hepatic IGF-I mRNA level (OD: GH-treated, 1·00±0·05; diabetic, 0·28±0·08; P<0·01), whilst having no effect on blood glucose values (diabetic, 36·35±0·45 mmol/l; GH-treated, 38·65±2·39 mmol/l). Additional administration of s.c. insulin completely restored IGF-I mRNA levels to those of controls (OD: low dose, 1·35±0·14; high dose, 1·27 ± 0·18). These observations indicate that insulin and GH are required for full expression of hepatic IGF-I mRNA and that insulin given i.p. is more potent than that given s.c. at stimulating hepatic synthesis of IGF-I.

scholarly journals Antidiabetic Activity ofVinca roseaExtracts in Alloxan-Induced Diabetic Rats

2010 ◽  
Vol 2010 ◽  
pp. 1-6 ◽  
Author(s):  
Mohammed Fazil Ahmed ◽  
Syed Mohammed Kazim ◽  
Syed Safiullah Ghori ◽  
Syeda Sughra Mehjabeen ◽  
Shaik Rasheed Ahmed ◽  
...  
Keyword(s):  
Body Weight ◽  
Plant Extract ◽  
Low Dose ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
High Dose ◽  
Vinca Rosea ◽  
Methanolic Extracts ◽  
Whole Plant ◽  
Histopathological Studies

The present study was carried out to evaluate the antidiabetic activity ofVinca roseamethanolic whole plant extracts in alloxan induced diabetic rats for 14 days. The methanolic whole plant extract at high dose (500 mg/kg) exhibited significant anti-hyperglycemic activity than whole plant extract at low dose (300 mg/kg) in diabetic rats. The methanolic extracts also showed improvement in parameters like body weight and lipid profile as well as regeneration ofβ-cells of pancreas in diabetic rats. Histopathological studies reinforce the healing of pancreas, by methanolicVinca roseaextracts, as a possible mechanism of their antidiabetic activity.

scholarly journals Hypoglycemic and Hepatorenal Effect of Ocimium gratissimium in Alloxan Induced Diabetic Rats

2021 ◽  
pp. 38-48
Author(s):  
Igwe Gloria ◽  
Nsirim Nduka ◽  
G. Tamunoemine Davies ◽  
Brown Holy
Keyword(s):  
Blood Glucose ◽  
Low Dose ◽  
Total Bilirubin ◽  
Diabetes Management ◽  
Fasting Blood Glucose ◽  
Diabetic Rats ◽  
Health Concern ◽  
High Dose ◽  
Dependent Manner ◽  
Glucose Reduction

Diabetes Mellitus is a disease of public health concern which is caused by pancreatic defect in insulin secretion or failure of the receptor cells to effectively utilize secreted insulin. Diabetes account for 2-3% death in the poorest countries hence the need for alternative control measure. This stud evaluated the hypoglycemic and hepatorenal effect of Ocimium gratissimium and glibenclamide in alloxan induced diabetic rats. Twenty- four rats were randomly divided into 6 groups of 4 animals in each group (1,2,3,4,5 & 6), groups 2,3,4,5 & 6 were induced diabetes intraperitoneally with 150 mg\kg alloxan (Sigma Ltd), diabetes was confirmed by fasting blood glucose of >10.0mmol/L. Groups 3,4,5 & 6 were subsequently treated with 400 mg/kg of extract, 5mg glibenclamide, 800 mg/kg of extract, 400 mg/kg extract combined with 5mg glibenclamide respectively. Blood glucose, hepatic function variables (Aspartate aminotransferase (AST), Alanine aminotransferase(ALT), Total bilirubin (TB) and renal parameters Sodium (Na+), Potassium (K+), Urea were analyzed. The result shows an increase in glucose, hepatic and renal parameters in diabetic induced groups which was significantly reduced in a dose dependent manner in the diabetic treated groups, the high dose of the extract (800mg/kg) was more effective in blood glucose reduction than the standard antidiabetic drug, (5mg glibenclamide). However, 5mg glibenclamide was found to be more effective in blood glucose reduction than the low dose (400mg/kg) extract, the combination of 5mg glibenclamide and 400mg/kg was found to be more effective in blood glucose reduction than the low dose extract. A significant increase was observed in the Total bilirubin and urea parameters of the high dose (800mg/kg) of the extract treated groups and in the combined group (400 mg/kg+5 mg glibenclamide). When compared to the low dose extract group(400mg/kg). Low dose ocimium gratissimium potentiates 5mg glibenclamide in blood glucose reduction. Ocimium gratissimium and glibenclamide decreased blood glucose and ameliorates alloxan induced hepatic and renal damage. The use of the high dose of the extract and the use of the combination of the drug (5mg glibenclamide) and the low dose of the extract in diabetes management may be detrimental to the liver and kidney according to this study.

scholarly journals Subacute Toxicity and Hepatoprotective Effects of Sarcocephalus latifolius in Alloxan Induced Diabetic Rats

2021 ◽  
pp. 24-37
Author(s):  
Olubunmi Simeon Oyekunle ◽  
Adewale Adetutu ◽  
Adijat Funke Ogundola
Keyword(s):  
Liver Disease ◽  
Low Dose ◽  
Diabetes Management ◽  
Inflammatory Cells ◽  
Diabetic Rats ◽  
The Body ◽  
Albino Rats ◽  
Diabetic Patients ◽  
High Dose ◽  
Leaf Powder

Various studies suggest that mortality due to liver disease in diabetic patients is very high; however, the recognition of DM as the primary cause of chronic liver disease is neglected in medical practice, we therefore evaluated the activities of Sarcocephalus latifolius leaf powder on the liver function of alloxan – induced diabetic rats. Forty-five healthy female albino rats were randomly assigned into 9 different groups; diabetes was induced intraperitonealy with 160 mg/kg of alloxan. Normal and diabetic rats were administered orally with 300, 600, 750 mg/kg/ b.w of S. latifolius. After 28 days, the animals were sacrificed for biochemical and histological studies. The body weight of the normal and diabetic rats increased significantly with S. latifolius treatment, the increase observed in the blood glucose was brought down upon treatment with S. latifolius leaf powder. The activity of ALT increased significantly with 750 mg/kg of S. latifolius leaf powder, while low dose of the plant decreased it significantly in diabetic rats. GGT activity only decreased in the diabetic rats treated with 300 mg/kg of S. latifolius whereas albumin increased significantly (p<0.05) in all the groups administered S. latifolius powder relative to the untreated diabetic group. Bilirubin concentration only increased significantly (p<0.05) in the group administered 750 mg/kg of S. latifolius leaf powder. Histological changes including infiltration of the sinusoids and focal area by inflammatory cells and mild portal congestion were observed in all the groups except the normal and diabetic rats treated with 300 mg/kg of S. latifolius leaf powder. The result of the study showed that S. latifolius could only be encouraged for diabetes management only at low dose and might be hepatotoxic at high dose.

scholarly journals MDA levels in the pancreas, testes, liver, and plasma of diabetic rats: The effect of snakehead (Channa striata) extract

2020 ◽  
Vol 12 (1) ◽  
Author(s):  
NURLITA ABDULGANI ◽  
DEWI HIDAYATI ◽  
RESSY ADINOVITASARY ◽  
VARAH OLIVIATIE ◽  
AYU DIAH SEKARTAJI
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Low Dose ◽  
Positive Control ◽  
Negative Control ◽  
Diabetic Rats ◽  
High Dose ◽  
Channa Striata ◽  
Negative Controls ◽  
Biomarkers Of Oxidative Stress

Abstract. Abdulgani N, Hidayati D, Adinovitasary R, Oliviatie V, Sekartaji AD. 2020. MDA levels in the pancreas, testes, liver, and plasma of diabetic rats: The effect of snakehead (Channa striata) extract. Nusantara Bioscience 12: 50-54. There are several biomarkers of oxidative stress in diabetes mellitus; one of those biomarkers is Malondialdehyde (MDA). Increasing oxidative stress will cause increased tissue damage. This study was conducted to determine the effect of snakehead extract (SHE) on MDA level of pancreas, testes, liver, and plasma of alloxan-induced diabetic rats. There were 5 groups of treatments: non-diabetic rats/negative control (C-), diabetic rats/positive control (C+), and 3 SHE level of administration: 1 mL/day (low dose/LD), 1.6 mL/day (middle dose/MD) and 2.1 mL/day (high dose/HD). Alloxan-induced diabetic rats were administered with SHE extract orally every day for two weeks. The results showed that MDA levels in the testes, liver, pancreas, and plasma of diabetic rats administered with SHE and non-diabetic rats/negative controls (C-) were significantly lower (p <0.05) compared to MDA levels in the testes, liver, pancreas, and plasma of diabetic rats without SHE administration (positive control/ C +). The highest dose of SHE treatment (2.1 mL/day) results in decreasing MDA levels were not significantly different (p> 0.05) with the group of non-diabetic rats / negative controls (C-). The conclusion of this study was increasing SHE administration up to 2.1 mL/day result in reducing more of MDA levels in plasma, pancreas, liver, and testes of diabetic rats.

Acetaminophen: Neonatal hepatotoxicity due to late pregnancy exposure

1987 ◽  
Vol 45 ◽  
pp. 718-719
Author(s):  
G.A. Miranda ◽  
M.A. Arroyo ◽  
C.A. Lucio ◽  
M. Mongeotti ◽  
S.S. Poolsawat
Keyword(s):  
Low Dose ◽  
Fetal Liver ◽  
Late Pregnancy ◽  
Toxic Chemicals ◽  
Control Group ◽  
High Dose ◽  
Over The Counter ◽  
Liver And Kidney ◽  
Neonatal Liver ◽  
Childbearing Women

Exposure to drugs and toxic chemicals, during late pregnancy, is a common occurrence in childbearing women. Some studies have reported that more than 90% of pregnant women use at least 1 prescription; of this, 60% used more than one. Another study indicated that 80% of the consumed drugs were not prescribed, and of this figure, 95% were “over-the-counter” drugs. Acetaminophen, the safest of all over-the-counter drugs, has been reported to induce fetal liver necrosis in man and animals and to have abortifacient and embryocidal action in mice. This study examines the degree to which acetaminophen affects the neonatal liver and kidney, when a fatty diet is simultaneously fed to the mother during late pregnancy.Timed Swiss Webster female mice were gavaged during late pregnancy (days 16-19) with fat suspended acetaminophen at a high dose, HD = 84.50 mg/kg, and a low dose, LD = 42.25 mg/kg; a control group received fat alone.

Evaluation of hippocampus in rhesus monkeys after chronic (1-year) inhalation exposure to marijuana: I. Electron microscopy

1990 ◽  
Vol 48 (3) ◽  
pp. 398-399
Author(s):  
A.M. Andrews ◽  
S.W. Wilson ◽  
A.C. Scallet ◽  
S.F. Ali ◽  
J. Bailey ◽  
...  
Keyword(s):  
Synaptic Vesicles ◽  
Rhesus Monkeys ◽  
Low Dose ◽  
Inhalation Exposure ◽  
Synaptic Cleft ◽  
High Dose ◽  
Withdrawal Time ◽  
Treatment Groups ◽  
Ultrastructural Evidence ◽  
Male Rhesus

Exposure of rhesus monkeys (Macaca mulatta) to marijuana via inhalation or to intravenous delta-9-tetrahydrocannabinol (THC), reportedly caused ultrastructural evidence of increased synaptic width. Chronic marijuana smoke in a single rhesus monkey examined after a six month withdrawal time caused ultrastructure changes in the septal, hippocampal and amygdala regions; the synaptic cleft was widened, electron opaque material was found in the cleft and in the pre- and postsynaptic regions, with some clumping of the synaptic vesicles. The objective of our study was to assess neuropathological alterations produced by chronic inhalation of marijuana smoke.Nineteen male rhesus monkeys, 3-5 years of age and weighing 3-8 kg, were divided into four treatment groups: a) sham control, b) placebo smoke (7 days/ week) c) low dose marijuana (2 times/week with 5 days/week sham) and d) high dose marijuana (7 times/week). A smoke exposure consisted of smoke from one cigarette (2.6% THC) burned down to 10 mm butt length. Smoke was administered via smoke generator (ADL II, Arthur D. Little, Inc. Cambridge, MA) and nose-mouth only masks (local production) equipped with one-way valves.

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