scholarly journals Green synthesis of silver nanoparticles by aqueous extract of Dovyalis abyssinica fruit for antibacterial applications

2021 ◽  
Vol 14 (3) ◽  
pp. 229-237
Author(s):  
Mekuriaw Assefa Kebede ◽  
Baye Sitotaw ◽  
Kidanemariam Teklay Hilawea
Keyword(s):  
Silver Nanoparticles ◽  
Plant Extract ◽  
Metallic Nanoparticles ◽  
Color Change ◽  
Citrus Fruit ◽  
Metallic Ions ◽  
E Coli ◽  
Colored Product ◽  
Absorbance Peak ◽  
Better Than

Green biosynthesis technique was employed to synthesize silver nanoparticles (AgNPs). Fresh citrus fruit of Dovyalis abyssinica (vernacular name Koshim) tree was extracted by distilled water to obtain phenolic natural compounds that have reducing capacity of metallic ions to the corresponding metallic nanoparticles, in this case, silver ion to silver nanoparticles. The appearance of the UV-Vis absorbance peak at 430 nm and the color change from pale yellow reaction mixture to reddish brown colored product solution have confirmed the formation of AgNPs. FTIR data has also shown the presence of organic components from the plant with the particles that may be used as capping agents to stabilize the formed particles and to control the size. The prepared nanoparticles and the plant extract have shown antibacterial property against E. coli and S. aureus, though the effect of the AgNPs was better than the plant extract. This study contributes for the development of environmentally friendly procedures in the preparation of nanoparticles for medicine, energy or environment.

Efficacy of Silver Nanoparticles Synthesized from Hymenocallis species (Spider Lilly) Leaf Extract as Antimicrobial Agents

2019 ◽  
Vol 12 (5) ◽  
pp. 4644-4647
Author(s):  
K.K. Gupta ◽  
Neha Kumari ◽  
Neha Sinha ◽  
Akruti Gupta
Keyword(s):  
Silver Nanoparticles ◽  
Leaf Extract ◽  
Antimicrobial Agents ◽  
Color Change ◽  
Bacterial Species ◽  
Antimicrobial Property ◽  
Biogenic Synthesis ◽  
E Coli ◽  
Light Yellow ◽  
Antibiotic Property

Biogenic synthesis of silver nanoparticles synthesized from Hymenocallis species (Spider Lilly) leaf extract was subjected for investigation of its antimicrobial property against four bacterial species (E. coli, Salmonella sp., Streptococcus sp. & Staphylococcus sp.). The results revealed that synthesized nanoparticles solution very much justify the color change property from initial light yellow to final reddish brown during the synthesis producing a characteristics absorption peak in the range of 434-466 nm. As antimicrobial agents, their efficacy was evaluated by analysis of variance in between the species and among the different concentration of AgNPs solution, which clearly showed that there was significant variation in the antibiotic property between the four different concentrations of AgNPs solution and also among four different species of bacteria taken under studies. However, silver nanoparticles solution of 1: 9 and 1:4 were proved comparatively more efficient as antimicrobial agents against four species of bacteria.

scholarly journals SIZE DEPENDENT APPLICATION OF BIOLOGICALLY SYNTHESIZED SILVER NANOPARTICLES AGAINST BACTERIAL SKIN PATHOGENS

2017 ◽  
Vol 10 (10) ◽  
pp. 192 ◽  
Author(s):  
Patil Sunita ◽  
Muthusamy Palaniswamy
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Plant Extract ◽  
Skin Infections ◽  
Bacterial Strains ◽  
Extract Concentration ◽  
Childhood Morbidity ◽  
Common Skin ◽  
Staphylococcus Infections ◽  
Absorbance Peak

  Objective: Bacterial skin infection is one of the most common causes of childhood morbidity in India. Mostly, it is caused by Streptococcus and Staphylococcus infections. However, because of antibiotic resistance in bacterial strains, treatment of skin infections is becoming increasingly difficult. The objective of this research is to study the effect of plant extract concentration on synthesis and morphology of biological silver nanoparticles and investigation of their activity against bacterial skin pathogens.Methods: Biological silver nanoparticles were synthesized using two concentrations (5 and 10 ml) of Aegle marmelos fruit pulp extract. Ultraviolet (UV)-visible spectroscopy, field emission scanning microscopy (FESEM), and high resolution transmission electron microscopy (HRTEM) were used to analyze morphological features of nanoparticles. Antibacterial activity of synthesized silver nanoparticles was studied against the most common skin pathogens Staphylococcus aureus and Streptococcus pyogen, using a well diffusion method.Results: The silver nanoparticles synthesized from 5 ml extract showed UV-absorbance peak at 430 nm with 14-18 nm size, while silver nanoparticles synthesized from 10 ml extract was showed the absorbance at 427 nm with 4-8 nm size. FESEM and HRTEM analysis revealed that both the silver nanoparticles were spherical in shape. Both nanoparticles have shown antibacterial activity among them silver nanoparticles synthesized from 10 ml extract showed better antibacterial activity.Conclusion: This research confirms that plant extract concentration modulate the rate of synthesis, morphology, surface plasmon resonance, and activity of biological silver nanoparticles. Silver nanoparticles synthesized from 10 ml extract can be used efficiently in the treatment of bacterial skin infections.

Biosynthesis of Silver Nanoparticles Using Plant Extract and its Anti-Plasmodial Property

2015 ◽  
Vol 1086 ◽  
pp. 11-30 ◽  
Author(s):  
Chellasamy Panneerselvam ◽  
Kadarkarai Murugan ◽  
Duraisamy Amerasan
Keyword(s):  
Silver Nanoparticles ◽  
Aqueous Extract ◽  
Plant Extract ◽  
Metallic Nanoparticles ◽  
Antimicrobial Properties ◽  
Nanoparticle Synthesis ◽  
Silver Ions ◽  
Antiplasmodial Activity ◽  
X Ray ◽  
Scanning Electron

Metallic nanoparticles have received great attention from chemists, physicists, biologists and engineers who wish to use them for the development of a new generation of nanodevices. In the present Communication, a completely “green” chemistry method for producing silver nanoparticles is introduced. The process is simple, environmentally benign, and quite efficient. Green nanoparticle synthesis has been achieved using environmentally acceptable plant extract and ecofriendly reducing and capping agents. In particular, silver nanoparticles are proved to have potential antibacterial, antifungal and antiplasmodial and antimicrobial properties. The present study was aimed to identify the antiplasmodial activity of green synthesised silver nanoparticles (AgNPs) using aqueous extract of plantEuphorbia hirtaagainstP.falciparum. Nanoparticles are being used in many commercial applications. It was found that aqueous silver ions can be reduced by aqueous extract of plant to generate extremely stable silver nanoparticles in water. The bio-reduced silver nanoparticles were appropriately characterized by UV–vis spectrum, Scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX) and X-ray diffraction (XRD). The formation of the AgNPs synthesized from the XRD spectrum compared with the standard confirmed spectrum of silver particles formed in the present experiments were in the form of nanocrystals, as evidenced by the peaks at 2θ values of =28.01°, 32.41°, 46.44°, 55.05° and 57.75°. The scanning electron micrograph (SEM) showed structures of spherical, cubic shape, and the size range was found to be 30–60 nm. The EDX spectra showed the purity of the material and the complete chemical composition of the synthesized AgNPs. The parasitic inhibition was dose-dependent. The synthesized AgNPs showed considerable antiplasmodial activity than the crude methanol and aqueous leaf extract ofE.hirta. The maximum efficacy was

scholarly journals Biogenic selenium nanoparticles (SeNPs) from citrus fruit have anti-bacterial activities

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ghalia Batool Alvi ◽  
Muhammad Shahid Iqbal ◽  
Mazen Mohammed Saeed Ghaith ◽  
Abdul Haseeb ◽  
Bilal Ahmed ◽  
...  
Keyword(s):  
Metallic Nanoparticles ◽  
Metal Salt ◽  
Citrus Fruit ◽  
Antimicrobial Activities ◽  
Selenium Nanoparticles ◽  
Biological Synthesis ◽  
Synthesis Of Nanoparticles ◽  
E Coli ◽  
Fruit Extracts ◽  
Physical And Chemical

AbstractNanotechnology deals with the synthesis of materials and particles at nanoscale with dimensions of 1–100 nm. Biological synthesis of nanoparticles, using microbes and plants, is the most proficient method in terms of ease of handling and reliability. Core objectives of this study were to synthesize metallic nanoparticles using selenium metal salt from citrus fruit extracts, their characterization and evaluation for antimicrobial activities against pathogenic microbes. In methodology, simple green method was implicated using sodium selenite salt solution and citrus fruit extracts of Grapefruit and Lemon as precursors for synthesizing nanoparticles. Brick red color of the solution indicated towards the synthesis of selenium nanoparticles (SeNPs). Nanoparticle’s initial characterization was done by UV–Vis Spectrophotometry and later FTIR analysis and DLS graphs via Zetasizer were obtained for the confirmation of different physical and chemical parameters of the nanoparticles. Different concentrations of SeNPs were used for antimicrobial testing against E. coli, M. luteus, B. subtilis and K. pneumoniae comparative with the standard antibiotic Ciprofloxacin. SeNPs possessed significant antimicrobial activities against all the bacterial pathogens used. Conclusively, SeNPs made from citrus fruits can act as potent antibacterial candidates.

Azadirachta Indica and Brassica Oleracea Mediated Green Synthesis vs. Chemical Synthesis of Silver Nanoparticles and their Antibacterial Properties

2019 ◽  
Vol 9 (3) ◽  
pp. 393-397
Author(s):  
R. Sharma ◽  
J. Singh ◽  
A.K. Bhatia
Keyword(s):  
Silver Nanoparticles ◽  
Brassica Oleracea ◽  
Plant Extract ◽  
Azadirachta Indica ◽  
Antibacterial Properties ◽  
Antibacterial Activities ◽  
Neem Extract ◽  
Alternative Source ◽  
Laser Scattering ◽  
E Coli

Introduction: An alternative source of synythesis of nanoparticles is plant extract rather than chemical methods. This is because of presence of secondary metabolites or reducing agents in plant extract which are responsible for nanoparticles synthesis. In bioaccumulation, this synthesis depends upon the availability of particular enzymes or protein in plant extract. Materials & Methods: Considering the therapeutic potentials of nanoparticles, this work has been designed to find out antibacterial activity of silver nanoparticles. Objectives of this work are - preparation of silver nanoparticles chemically and biologically, characterisation of nanoparticles and evaluation of their antibacterial activities against E. coli. Comparision of antibacterial properties were made among NaBH4- AgNPs, Azadirachta indica (Neem) extract AgNPs and Brassica oleracea (Cauliflower) extract AgNPs. UV- absorption spectra of chemically and biologically synthesized AgNPs at different time intervals were measured using UV-Visible spectrophotometer. Particle size of AgNPs was measured by dynamic laser scattering technique (DLS) using Malvern Aimil Zetasizer. Results: The obtained silver nanoparticles were of sizes between 10 nm and 100 nm. Conclusion: It was clear from antibacterial activities that biologically synthesized AgNPs were more effective against E. coli than chemically synthesized AgNPs.

scholarly journals EVALUATION OF PHYTOCHEMICAL CONSTITUENTS AND ANTIMICROBIAL ACTIVITY OF SILVER NANOPARTICLE SYNTHESIZED IPOMOEA NIL AGAINST SELECTED PATHOGENS

2017 ◽  
Vol 10 (3) ◽  
pp. 183
Author(s):  
Asha Sivaji ◽  
Asha A ◽  
Rajeshkumar S
Keyword(s):  
Silver Nanoparticles ◽  
Aqueous Extract ◽  
Pathogenic Bacteria ◽  
Color Change ◽  
Silver Ions ◽  
Inhibition Zone ◽  
E Coli ◽  
Ipomoea Nil ◽  
Inhibition Zones ◽  
Phytochemical Constituents

ABSTRACTObjective: The objective of this study is to investigate a simple and feasible approach for the production of silver nanoparticles (AgNPs) by using leafextract of Ipmoea nil and to evaluate its antibacterial activity.Methods: The AgNPs synthesized was characterized by a change in color and the absorption peaks by ultraviolet-visible spectroscopy. The phytocompounds responsible for the reduction and capping of silver ions was known from Fourier transform infrared spectra and phytochemical analysis.The antibacterial effects of prepared aqueous AgNPs were detected against five types of pathogenic bacteria, including Gram-negative and Grampositivebacteria (Escherichia coli, Salmonella, Bacillius, Proteus, and Klebsilla pneumonia) using agar well diffusion method.Results: A peak absorption value between 400 and 450 nm and the color change of the extract from yellowish to red wine were corresponds tothe plasmon absorbance of AgNPs. On the other hand, aqueous extract of I. nil mediated AgNPs found to be effective against tested microorganisms(Salmonella, Bacillus, and Proteus) with inhibition zone in the range of 10-13 mm (20 μl and 10 μl) except E. coli and K. pneumonia. Furthermore,aqueous extract of I. nil leaves had no ability to suppress the growth of the tested microorganisms in the concentration of 10 μl. The control alsoproduced similar inhibition zones like AgNPs.Conclusions: Our findings indicated that green synthesized AgNPs mediated by I. nil leaf extract had an efficient anti-bactericidal activity against thebacterial species tested. Hence, further studies are needed to highlight its mechanism and application as an antibacterial agent.Keywords: Ipomoea nil, Silver nanoparticles, Antibacterial activity, Bacteria.

scholarly journals Green Synthesis, Characterization and Antibacterial Activity of Silver Nanoparticles from Capparis Spinosa Leaf Extract

2021 ◽  
Vol 72 (1) ◽  
pp. 145-152
Author(s):  
Saleh H. Salmen ◽  
Eman Damra ◽  
Tahani Awad Alahmadi ◽  
Sulaiman Ali Alharbi
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Green Synthesis ◽  
Plant Extract ◽  
Leaf Extract ◽  
Average Particle ◽  
Surface Plasmon Resonance Peak ◽  
Plasmon Resonance Peak ◽  
E Coli ◽  
Capparis Spinosa

The current study reports the green synthesis of silver nanoparticles (AgNPs) using Capparis spinosa leaf extract acting as a capping and reducing agent. The characterization of AgNPs was confirmed using ultraviolet-visible spectrophotometry (UV-Visible), fourier transform infrared spectroscopy (FTIR), energy-dispersive X-ray spectroscopy (EDX), and transmission electron microscopy (TEM). The plant extract used reduces Ag+ into AgNPs within a few minutes as indicated by the changed color, from yellow to reddish-brown. The UV-vis spectrum of AgNPs appeared a characteristic surface plasmon resonance peak at 400-450 nm. FTIR spectroscopy confirmed the role of plant extract as a reducing and capping agent of silver ions. The spectra of FTIR revealed a broad transmission peaks from 3412 to 617 cm-1. An EDX analysis signal at 3 keV and weight 65.38% showed the peak to be in the silver region, a fact which was confirmed by the presence of elemental silver. Under TEM, the nanoparticles were seen to be spherical, with an average particle size of 13 nm. AgNPs showed antibacterial activity against S.epidermidis, S. aureus, MRSA and E. coli. The inhibition zones for S.epidermidis and S. aureus were 8 to 10 mm, while MRSA is 7 to 10 mm. The inhibition zone of E. coli was higher at 10 to 13 mm.

Silver Nanoparticles from Tabernaemontana Divaricate leaf extract: Mechanism of action and Bio-application for photo degradation of 4-Aminopyridine.

2021 ◽  
Author(s):  
Pratibha Attri ◽  
Sangeeta Garg ◽  
Jatinder Kumar Ratan ◽  
Ardhendu Sekhar Giri
Keyword(s):  
Silver Nanoparticles ◽  
Plant Extract ◽  
Leaf Extract ◽  
Enol Form ◽  
Optimum Conditions ◽  
Ag Nps ◽  
Photo Degradation ◽  
Xps Spectra ◽  
E Coli ◽  
Average Size

Abstract Silver nanoparticles (Ag NPs) were synthesised by the reduction of Ag+ to Ag0 in the presence of enol form of flavonoids present in plant extract of Tabernaemontana Divaricate (T. Divaricate). Prepared Ag NPs were characterized using UV-Vis, XRD, HR-TEM with EDX and XPS techniques. XPS spectra exhibited peaks at 366 eV and 373 eV, which specified spin orbits for Ag 3d3/2, and Ag 3d5/2 that confirmed the formation of Ag NPs. Ag NPs were spherical in shape with an average size of 30 nm as revealed by HR-TEM and FE-SEM techniques. EDX studies verified the high purity of Ag NPs with silver 46.96 %, carbon 16.35 %, oxygen 16.22 %, nitrogen 20.25 % and sulphur 0.21%. LC-MS analysis of plant extract confirmed the qualitative presence of alkaloids, tannins, flavonoids, phenols, and carbohydrates. Prepared Ag NPs showed good photocatalytic activity towards degradation of 4-Amniopyridine with 61% degradation efficiency at optimum conditions in 2 hrs of reaction time under visible light. The number of intermediates were found were ten within the mass number of 0–450. Ag NPs synthesized using bio-extract have also showed good inactivation against Escherichia Coli (E. Coli) and Bacillus Subtilis (B. Subtilis) bacteria due to availability of free radicals.

scholarly journals Dose-Dependent Antimicrobial Activity of Silver Nanoparticles on Polycaprolactone Fibers against Gram-Positive and Gram-Negative Bacteria

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Erick Pazos-Ortiz ◽  
Jose Hafid Roque-Ruiz ◽  
Efrén Amador Hinojos-Márquez ◽  
Juan López-Esparza ◽  
Alejandro Donohué-Cornejo ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Metallic Nanoparticles ◽  
Antibacterial Properties ◽  
Electrospinning Process ◽  
Drug Resistant ◽  
Gram Positive ◽  
Gram Negative ◽  
E Coli ◽  
Dose Dependent

The adhesion ability and adaptability of bacteria, coupled with constant use of the same bactericides, have made the increase in the diversity of treatments against infections necessary. Nanotechnology has played an important role in the search for new ways to prevent and treat infections, including the use of metallic nanoparticles with antibacterial properties. In this study, we worked on the design of a composite of silver nanoparticles (AgNPS) embedded in poly-epsilon-caprolactone nanofibers and evaluated its antimicrobial properties against various Gram-positive and Gram-negative microorganisms associated with drug-resistant infections. Polycaprolactone-silver composites (PCL-AgNPs) were prepared in two steps. The first step consisted in the reduction in situ of Ag+ions using N,N-dimethylformamide (DMF) in tetrahydrofuran (THF) solution, and the second step involved the simple addition of polycaprolactone before electrospinning process. Antibacterial activity of PCL-AgNPs nanofibers againstE. coli,S. mutans,K. pneumoniae,S. aureus,P. aeruginosa, andB. subtiliswas evaluated. Results showed sensibility ofE. coli,K. pneumoniae,S. aureus, andP. aeruginosa, but not forB. subtilisandS. mutans. This antimicrobial activity of PCL-AgNPs showed significant positive correlations associated with the dose-dependent effect. The antibacterial property of the PCL/Ag nanofibers might have high potential medical applications in drug-resistant infections.

scholarly journals Comparison of the antibacterial activity against Escherichia coli of silver nanoparticle produced by chemical synthesis with biosynthesis

2018 ◽  
Vol 2 (2) ◽  
Author(s):  
Nguyen Phuc Quan 1,2 ◽  
Tran Quoc Vinh 1 ◽  
Kieu Thi My Yen 1 ◽  
Le Vu Khanh Trang 2 ◽  
Nguyen Minh Ly 2 ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Color Change ◽  
Chemical Reduction ◽  
Spherical Shape ◽  
Average Diameter ◽  
Ag Nps ◽  
E Coli ◽  
Vis Spectroscopy

The synthesis of silver nanoparticles (Ag NPs) has been carried out using different methods, mainly by biological and chemical methods; however, comparing antibacterial activity of Ag NPs synthesized by these methods has not been conducted before. In this study, silver nanoparticles (Ag NPs) were synthesized by methods using reducing agent NaBH4/carboxymethyl cellulose (CMC) and fungal strain Trichoderma asperellum (T.asperellum). The formation of silver nanoparticles was observed visually by color change and identified by Ultraviolet-visible (UV – vis) spectroscopy. The transmission electron microscopy (TEM) image illustrated almost nanoparticles with spherical shape and average diameter of 4.1 ± 0.2 nm and 2.1 ± 0.2 nm of samples produced from chemical reduction and biosynthesis respectively. Both samples after 180 days storing have been separated lightly, but the agglomeration and absorbance peak shifting were not observed which proved the high stability of synthesized Ag NPs. Antimicrobial activity against human bacterial pathogen Escherichia coli (E. coli) showed that the inhibition zone produced by “biosynthesis” and “chemical reduction” Ag NPs were 3.17 cm and 2.42 cm respectively. With nanoparticles size smaller than 2 mm, antibacterial activity of “biosynthesis” Ag NPs against E. coli was 31 % higher than “chemical reduction” Ag NPs, although the concentration of Ag NPs produced by biosynthesis was about 10-fold less.

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