scholarly journals EVALUATION OF BIOACTIVITY OF JAGGERY PREPARED USING PLANT MUCILAGE AS CLARIFICANT

2018 ◽  
Vol 11 (11) ◽  
pp. 294
Author(s):  
Harish Nayaka Ma ◽  
Lava Chikkappaiah ◽  
Venkatesh Ks ◽  
Gunashree Bs ◽  
Sudharshan S
Keyword(s):  
Antibacterial Activity ◽  
Aloe Vera ◽  
Reducing Power ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Total Phenol Content ◽  
Total Flavonoids ◽  
Death Time ◽  
Reducing Power Assay

Objective: The aim of the study was to prepare the jaggery using plant mucilage as clarificants and to evaluate the bioactivity of jaggery by determining total phenols, total flavonoids, reducing power, antibacterial activity, and antihelmintic activity.Methods: Jaggery was prepared from sugarcane variety Co-80632 using five plant mucilage as clarificants, namely Aloe vera, flax seeds, fenugreek, purslane, and malabar spinach at three different concentrations, i.e., 0.1%, 0.2%, and 0.4% of raw sugarcane juice. The characteristic bioactivities such as total phenol content by Folin–Ciocalteu method, flavonoids content by spectrophotometer method, reducing power assay by measuring the absorbance at 700 nm, antibacterial activity by well-diffusion method, and antihelmintic activity by in vitro were determined.Results: The highest total phenolic content was observed in jaggery prepared using plant mucilage clarificant of A. vera (JAV4 [3.68±0.02]) and Fenugreek seeds (JFG4 [3.61±0.02]) at 0.4% followed by them at 0.2% and 0.1% concentration, respectively. Similarly, a significant amount of total flavonoids content was elevated only in 0.4% plant mucilage clarificants of Aloe vera-treated experimental group jaggery (0.73±0.01) compared to control (0.61±0.02). Further, the antioxidant activity of jaggery was evaluated by reducing power. The increased absorbance at 700 nm indicated the presence of reducing power. At 10 mg/ml concentration, an absorbance unit of 1.93 and 1.80 was observed for jaggery prepared using 0.4% concentration of A. vera and fenugreek seed mucilage clarificants. Antihelmintic activity of jaggery showed dosage depended on the pattern where with the increase in the concentration of mucilage, the antihelmintic activity enhanced. A. vera mucilage showed maximum antihelmintic activity at 0.4% concentration with paralytic time (28.6 min) and death time (39.6 min).Conclusion: Jaggery prepared using plants mucilaginous clarificants is the richest source of polyphenols such as phenolic acids and flavonoids, thereby contributed to the enhancement of bioactivity such as antioxidant, antibacterial, and antihelmintic activity. Thus, jaggery prepared using plant mucilage as clarificants is a novel nutraceutical product which can supplement the nutrients along with various medicinal properties through antioxidant, antibacterial, and antihelmintic activity.

scholarly journals Phytochemical Screening, Polyphenols Content, Antioxidant Power, and Antibacterial Activity of Herniaria hirsuta from Morocco

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Kenza Ammor ◽  
Dalila Bousta ◽  
Sanae Jennan ◽  
Bahia Bennani ◽  
Abdellah Chaqroune ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Antioxidant Power ◽  
Negative Effect ◽  
Hydroethanolic Extract

The aim of this study is to investigate in vitro antioxidant and antibacterial activities of the aqueous and hydroethanolic extracts for aerial parts of Herniaria hirsuta. Extracts were screened for their possible antioxidant activities by three tests: DPPH free radical-scavenging, reducing power, and molybdenum systems. The screening of antibacterial activity of extracts was individually evaluated against sixteen bacteria species using a disc diffusion method. Flavonoids, total phenols, and tannins content were performed for both extracts. It shows higher content in the hydroethanolic extract. The hydroethanolic extract showed a significant antioxidant activity for the three methods studies to the aqueous extract, but nonsignificant results compared to the reference (BHT). However, both extracts have negative effect on the strains studies for the antibacterial activity.

scholarly journals CrudeAloe veraGel Shows Antioxidant Propensities and Inhibits Pancreatic Lipase and Glucose MovementIn Vitro

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Urmeela Taukoorah ◽  
M. Fawzi Mahomoodally
Keyword(s):  
Pancreatic Lipase ◽  
Antioxidant Properties ◽  
Aloe Vera ◽  
Reducing Power ◽  
Kinetic Studies ◽  
Total Phenolic ◽  
Anthocyanin Content ◽  
Maximal Velocity ◽  
Inhibitory Potential

Aloe veragel (AVG) is traditionally used in the management of diabetes, obesity, and infectious diseases. The present study aimed to investigate the inhibitory potential of AVG againstα-amylase,α-glucosidase, and pancreatic lipase activityin vitro. Enzyme kinetic studies using Michaelis-Menten (Km) and Lineweaver-Burk equations were used to establish the type of inhibition. The antioxidant capacity of AVG was evaluated for its ferric reducing power, 2-diphenyl-2-picrylhydrazyl hydrate scavenging ability, nitric oxide scavenging power, and xanthine oxidase inhibitory activity. The glucose entrapment ability, antimicrobial activity, and total phenolic, flavonoid, tannin, and anthocyanin content were also determined. AVG showed a significantly higher percentage inhibition (85.56±0.91) of pancreatic lipase compared to Orlistat. AVG was found to increase the Michaelis-Menten constant and decreased the maximal velocity (Vmax) of lipase, indicating mixed inhibition. AVG considerably inhibits glucose movement across dialysis tubes and was comparable to Arabic gum. AVG was ineffective against the tested microorganisms. Total phenolic and flavonoid contents were66.06±1.14 (GAE)/mg and60.95±0.97 (RE)/mg, respectively. AVG also showed interesting antioxidant properties. The biological activity observed in this study tends to validate some of the traditional claims of AVG as a functional food.

scholarly journals In-vitro Antioxidant Activity and Preliminary Phytochemical Analysis of Different Leaf Extracts of Hemionitis arifolia

2021 ◽  
pp. 281-291
Author(s):  
Rajendran Raja Priya ◽  
N. Bhadusha ◽  
Veramuthu Manivannan ◽  
Thanthoni Gunasekaran
Keyword(s):  
Nitric Oxide ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Hydroalcoholic Extract ◽  
Reducing Power Assay

Objective: To evaluate the preliminary phytochemical content and antioxidant potential of the hydroalcoholic leaf extracts of Hemionitis arifolia. Methods: Total phenolic, flavonoid and alkaloid contents were evaluated using spectrophotometric methods. The free radical scavenging activity of the leaf hydroalcoholic extract were evaluated against DPPH+, ABTS+, Reducing power assay and nitric oxide assay were determined. Results: The hydroalcoholic concentrate of H. arifolia uncovered the most elevated polyphenol content when contrasted and the other phytoconstituents. Absolute phenol content of the hydroalcoholic separate was observed to be 31.78%, flavonoid content is 1.02% and Alkaloid content is 30.40% individually. The Solvent concentrates showed huge cell reinforcement movement, with hydroalcoholic extract. ABTS Assay, DPPH assay, Reducing power assay and Nitric oxide assay where the Inhibition concentration were 667.75µg/ml, 734.25 µg/ml, 791.58 µg/ml and 899.67 µg/ml. Conclusion: This study suggests that hydroalcoholic leaf extracts of H. arifolia could be a potential source of natural antioxidant and justifies its use in ethno-medicine.

scholarly journals Phytochemical, Antioxidant and Antimicrobial studies of Lannea egregia Engl. & K. Krause (Anacardiaceae) extracts and chromatographic fractions

2020 ◽  
Vol 19 (1) ◽  
pp. 348-363
Author(s):  
Philip A. Idowu ◽  
Loveth C. Ekemezie ◽  
Charles O. Olaiya
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Antioxidant Capacity ◽  
Reducing Power ◽  
Stem Bark ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
Crude Extracts ◽  
Different Parts

Screening ‘new’ medicinal plants of traditional importance for bioactive components is a sure way of discovering novel therapeutic agents to treat diseases. This study, therefore investigated the presence of phytochemical, antioxidant and antibacterial components of the extracts of Lannea egregia. Phytochemical screening was done by standard methods. Antibacterial activity of the extracts of Lannea egregia was determined by agar well diffusion method while the minimum inhibitory concentration (MIC) was determined by agar dilution method. The antioxidant capacity of the crude extracts was determined through the evaluation of total flavonoid content, total phenolic content, ferric reducing power, total antioxidant capacity and 2,2-diphenyl-1-picryhydrazyl. The phytochemical screening of the different parts of this plant revealed the presence of tannins, saponins, alkaloids, flavonoids, steroids, terpenoids, emodins, phlobatannins, anthocyanins, coumarins and phenolics. Phlobatannins was observed to be absent in the stem bark. The crude extracts obtained from the leaves, stem bark and roots of this plant exhibited good antibacterial activity against typed strains of Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa and Escherichia coli. The diameter of the zone of inhibition ranged from 9.0 to 26.0 mm at 100 mg/mL for all the plant parts. The ethyl acetate leaf extract of this plant possessed the highest antibacterial activity with MIC and MBC values of the range of (3.125 to ˃50 mg/mL) and (12.5 to ˃50 mg/mL) respectively. The zone inhibition of the chromatographic fractions of both plants ranged 15-23 mm. Antioxidant study of the extracts of the leaf of L. egregia revealed that the ethyl acetate and methanol extracts have good antioxidant potentials comparable to that of ascorbic acid control. This study has revealed that the extracts from different parts of L. egregia possess good antibacterial and antioxidant activities which could be a function of the various phytochemicals detected in the plant. Keywords: Lannea egregia, Phytochemical, Antibacterial, Antioxidant, Column chromatography.

scholarly journals Eleucine indicaPossesses Antioxidant, Antibacterial and Cytotoxic Properties

2011 ◽  
Vol 2011 ◽  
pp. 1-6 ◽  
Author(s):  
Adel S. Al-Zubairi ◽  
Ahmad Bustamam Abdul ◽  
Siddig Ibrahim Abdelwahab ◽  
Chew Yuan Peng ◽  
Syam Mohan ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Human Cancer ◽  
Antioxidant Properties ◽  
Antibacterial Properties ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Significant Activity ◽  
Human Cancer Cell Lines

The use of evidence-based complementary and alternative medicine is increasing rapidly.Eleucine indica(EI) is traditionally used in ailments associated with liver and kidneys. The therapeutic benefit of the medicinal plants is often attributed to their antioxidant properties. Therefore, the aim of this study was to screen the hexane, dicholoromethane, ethyl acetate (EA) and methanol extracts (MeTH) of EI for their antioxidant, antibacterial and anti-cancer effects using total phenolic contents (TPCs) and DPPH, disc diffusion method and MTT cytotoxicity assays, respectively. The MeTH was showed to have the highest TPC and scavenging activity (77.7%) on DPPH assay, followed by EA (64.5%), hexane (47.19%) and DCM (40.83%) extracts, whereas the MeTH showed no inhibitory effect on all tested bacteria strains. However, the EA extract exhibited a broad spectrum antibacterial activity against all tested bacteria exceptBacillus subtilis, in which this bacterium was found to be resistant to all EI extracts. Meanwhile, hexane extract was demonstrated to have a remarkable antibacterial activity against methicillin resistantStaphylococcus aureus(MRSA) andPseudomonas aeruginosa, while the dicholoromethane extract did not exhibit significant activity againstP. aeruginosa. None of the extracts showed significant cytotoxic activity towards MCF-7, HT-29 and CEM-SS human cancer cell lines after 72 h incubation time (IC50> 30 μg/ml). These results demonstrate that the extract prepared from the EI possesses antioxidant activityin vitroin addition to antibacterial properties. Further investigations are needed to verify the antioxidant effectsin vitroandin vivo.

scholarly journals Ophiorrhiza mungos var. angustifolia – Estimation of camptothecin and pharmacological screening

2018 ◽  
Vol 5 (3) ◽  
pp. 113-120 ◽  
Author(s):  
G Krishna Kumar ◽  
A Muhammed Fayad ◽  
A Jayakumaran Nair
Keyword(s):  
High Performance ◽  
Pathogenic Bacteria ◽  
Methanol Extract ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Dry Weight ◽  
Total Phenolic ◽  
Therapeutic Principles ◽  
Reducing Power Assay

Ophiorrhiza mungose var. angustifolia (Thwaites) Hook. f (Family- Rubiaceae) is a recently identified plant from Ophiorrhiza species in Western Ghats of Kerala. The plant is a promising candidate for the production of camptothecin (CPT) - a high value anticancer compound. Preliminary screening of hexane and methanol extract revealed the presence of phenolics, flavonoids, caumarins, steroids, terpeanoids, saponins, carbohydrates and alkaloids. Camptothecin was estimated from methanol extract using high performance liquid chromatography and the level of CPT was 297.94 ± 2.27 µg/g dry weight. The in vitro antioxidant assay revealed both extract showed moderate level of total phenolic content, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, ferric chloride reducing power assay, phospho-molybdate assay of total anti-oxidant capacity and nitric oxide scavenging activity assay. Antimicrobial study reveals that only hexane extract inhibits pathogenic bacteria and fungus. Overall these findings will lead to isolation of active compounds other than camptothecin, elucidate them against wider range of bioactivity studies to find new therapeutic principles.

scholarly journals Antioxidant Activity and Antibacterial Effects on Clinical IsolatedStreptococcus suisandStaphylococcus intermediusof Extracts from Several Parts ofCladogynos orientalisand Their Phytochemical Screenings

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Pongtip Sithisarn ◽  
Piyanuch Rojsanga ◽  
Patchima Sithisarn ◽  
Sumet Kongkiatpaiboon
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
High Performance ◽  
Total Flavonoid ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Leaf Extracts ◽  
Spectrophotometric Methods ◽  
Staphylococcus Intermedius

Thein vitroantioxidant and antibacterial assays against clinically isolatedStreptococcus suisandStaphylococcus intermediusof the extracts prepared by decoction and ethanolic reflux of different parts of Chettaphangki (Cladogynos orientalisZipp. ex Span), including the leaves, roots, and stems, using 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging assay and disc diffusion method were conducted. Quantitative analysis of total phenolic and total flavonoid contents in the extracts using spectrophotometric methods was also performed. Finally, phytochemical screening by thin layer chromatography (TLC) and high performance liquid chromatography (HPLC) was conducted. Leaf ethanolic reflux extract (100 g) contained the highest total phenolic and total flavonoid contents of 7.21 ± 0.28 μg gallic acid equivalent (GAE) and 11.51 ± 2.02 μg rutin equivalent (RE), respectively. Chettaphangki extracts promoted low antioxidant activity with EC50values in the range of 0.27–0.48 mg/mL. Extracts and fractions from the roots and stems of this plant promoted low to intermediate antibacterial activity againstS. intermediuswith the inhibition zones between 7 and 14 mm. The chromatographic data suggested that the leaf extracts ofC. orientaliscontained rutin while the root and stem extracts contained scopoletin and chettaphanin I. Rutin promoted strong antioxidant activity while chettaphanin I showed low antibacterial activity againstStaphylococcus intermedius.

scholarly journals Effects of Development and Ripening Stage on Phytochemical Compositions, Antioxidant and Antibacterial Activities of Date Palm Fruits

2019 ◽  
Vol 32 (2) ◽  
pp. 415-419
Author(s):  
F.Z. Bentebba ◽  
Z. Ghiaba ◽  
M. Saidi ◽  
C. Bensasi
Keyword(s):  
Antioxidant Activity ◽  
Reducing Power ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Disk Diffusion Method ◽  
Maturation Stages ◽  
Palm Fruits ◽  
Edible Parts

Edible parts of two dates cultivars (Deguel ahmar and Tanslit) at four maturation stages khellal, besser, rutab and tamr were analyzed for their phytochemical composition (total phenolic, total flavonoid and condensed tannins contents) as well as antioxidant and antibacterial activities. The antioxidant activity evaluated in vitro using scavenging assays of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and 2,2′-azino-bis(3- ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical and ferric reducing power. Antibacterial activity of extracts were evaluated in vitro by the agar disk diffusion method against five pathogenic bacterias trains: Bacillus subtilis ATCC 6633, Enterococcus ATCC 3315, Staphylococcus aureus ATCC 43300, Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 7320. Results suggested that the content of these phytochemicals are very important at khellal stage and then decreased during maturation followed by a decrease in the antioxidant activity.

scholarly journals ANTIOXIDATIVE AND FREE RADICAL SCAVENGING POTENTIALS OF CYCLOSORUS INTERRUPTUS (WILLD.) H. ITÔ AND PRONEPHRIUM NUDATUM (ROXB. EX GRIFF.) HOLTTUM

2019 ◽  
pp. 87-94
Author(s):  
ABHIJIT MITRA ◽  
MANABENDRA DUTTA CHOUDHURY ◽  
PRAKASH ROY CHOUDHURY ◽  
DEEPA NATH ◽  
SUBRATA DAS ◽  
...  
Keyword(s):  
Methanol Extract ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Natural Antioxidants ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
In Vitro Systems ◽  
Reducing Power Assay ◽  
Phenolic And Flavonoid

Objectives: The work aims to screen the antioxidative potentials of different crude extracts of the fronds of two medicinally important pteridophytes of Southern Assam, India, namely, Cyclosorus interruptus (Willd.) H. Itô and Pronephrium nudatum (Roxb. ex Griff.) Holttum. Methods: Frond extracts of the pteridophytes were prepared by Soxhlet hot extraction method. Total phenolic content (TPC) and total flavonoid content (TFC) of the hexane, ethyl acetate, acetone, and methanol extracts of the fronds of the plants were done by following standard protocol. In vitro assessment of the antioxidative behavior of the extracts was performed using standard 2,2-diphenyl-1-picrylhydrazyl scavenging assay, reducing power assay, metal chelating assay, hydroxyl, superoxide, and 2,2’- azino-bis (3-ethylbenzothiazoline - 6 – sulfonic acid) radical scavenging methods in different in vitro systems. Results: Preliminary phytochemical analysis implicated the presence of phenolic and flavonoid compounds in all the frond extracts. The methanol extract of the fronds of both the plants showed maximum phenolic and flavonoid contents in comparison to the other extracts, however, that of C. interruptus was found to be higher than P. nudatum. Antioxidative potentials of the said extracts were also found to be impressive and noteworthy. The decreasing order of the antioxidative efficacies of the extracts was found to be same as that of TPC and TFC of the extracts. Conclusion: It is pertinent to comment that the methanol extract of the fronds of both the plants may be treated as a potential source of natural antioxidants.

scholarly journals Evaluation ofIn VitroAntioxidant Properties of Methanol and Aqueous Extracts ofParkinsonia aculeataL. Leaves

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Sonia Sharma ◽  
Adarsh Pal Vig
Keyword(s):  
Reducing Power ◽  
Thiobarbituric Acid ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Aqueous Extracts ◽  
Parkinsonia Aculeata ◽  
Phenolic Contents ◽  
Reducing Power Assay

In the present study, methanol and aqueous extracts ofParkinsonia aculeataL. leaves were prepared and analyzed for phytochemical analysis and antioxidant potential in differentin vitroassays. Antioxidant activity was studied using DPPH, CUPRAC, reducing power assay, deoxyribose degradation (site and nonsite specific), ferric reducing antioxidant potential (FRAP), ferric thiocyanate (FTC), thiobarbituric acid (TBA), and molybdate ion reduction, respectively. The total phenolic contents of the methanol and aqueous leaf extract were 39 mg GAE/g and 38 mg GAE/g, whereas flavonoid contents of these extracts were found to be 0.013 mg RE/g and 0.006 mg RE/g, respectively. From the two extracts, the methanol extract shows maximum inhibition (%) of 57.82%, 71.23%, 48.26%, 69.85%, and 52.78% in DPPH, nonsite- and site-specific, FTC, and TBA assays and absorbance of 0.669 and 0.241 in reducing power and CUPRAC assays at the highest concentration tested. UPLC analysis was done to determine the presence of various types of polyphenols present in plant extracts.

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