scholarly journals Mortality of Clarias gariepinus caused by Aeromonas caviae and nitrite toxicity in a fish farm

2021 ◽  
Vol 19 (2) ◽  
pp. 138-144
Author(s):  
O.O. Oladele ◽  
N.O. Ameji ◽  
G.Y. Gurumyen ◽  
W.A. Adanu ◽  
T.T. Kolade ◽  
...  
Keyword(s):  
Water Samples ◽  
Pathogenic Bacteria ◽  
Clarias Gariepinus ◽  
Fish Farming ◽  
Fish Pond ◽  
African Catfish ◽  
Fish Farms ◽  
Commercial Fish ◽  
Aeromonas Caviae ◽  
Nitrite Toxicity

In Nigeria, farming of freshwater fish, mainly that of Clarias gariepinus has gained prominence as means of improved protein supply and livelihood. Many farmers suffer untold losses in their bid to make a living from commercial fish production. Among the major causes of infectious disease outbreaks in fish farms are pathogenic bacteria of the genus Aeromonas. This is a case report of outbreak of Aeromonas caviae infection complicated by nitrite toxicity in Clarias gariepinus. Carcasses of 17 African catfish juveniles and two water samples were presented to the poultry and fish clinic of the Veterinary Teaching Hospital, University of Jos for investigation. The fishes had been on medication (Fish cure antibiotic +®) for over 5 days with cumulative mortality of 230 out of 2,500 fishes (9.2 %). Necropsy was conducted and samples were taken for microbial analysis and histopathology while water samples were subjected to chemical analysis. Gross lesions were broken barbels, cutaneous depigmentation, branchial pallor, ascites, renal and splenic congestion. Histologically, there was dissociation of hepatic cords, necrosis of hepatocytes with vacuolation and mononuclear cellular infiltrations. Also, there was renal tubular epithelial necrosis with mononuclear and heterophilic infiltration while micro abscesses were observed in the brain. Marked Zenker’s necrosis and edema were seen in the skeletal muscles. Organism isolated from livers and kidneys was identified as Aeromonas caviae and was susceptible to enrofloxacin, furaltadone and florfenicol. Nitrite in fish pond water was 1mg/L. Bath medication with enrofloxacin at 33mg/litre of water for 6 hours treatment per day was done to reduce the mortality. The farmer was advised to change the source of water for the fish pond immediately. It is recommended that to avoid losses in fish farming, farmers should check the quality of water intended for use on fish farms and ensure early health check on fingerlings purchased for rearing.

Antibiotic Resistance Profiling of Salmonella sp. isolated from African Catfish (Clarias gariepinus)

2021 ◽  
Vol 16 (3) ◽  
pp. 44-47
Author(s):  
P.T. Fowoyo
Keyword(s):  
Antibiotic Resistance ◽  
Pathogenic Bacteria ◽  
Clarias Gariepinus ◽  
Fish Farming ◽  
Selective Medium ◽  
Diffusion Method ◽  
African Catfish ◽  
Fresh Water Fish ◽  
Peptone Water ◽  
Amoxicillin Clavulanic Acid

The African catfish (Clarias gariepinus) is an important fresh water fish consumed by a large percentage of the populace globally and it may be contaminated by pathogenic bacteria such as Salmonella sp. In this study, a total of fifteen (15) samples of African catfish were collected from different markets in Lokoja, Nigeria. The Salmonella sp. were isolated from the catfish samples by pre-enrichment in peptone water and subsequent inoculation on selective medium namely brilliant-green agar (BGA), bismuth sulphite agar (BSA) and Salmonella-Shigella agar (SSA). The Salmonella isolates were tested for susceptibility to 10 different commercially available antibiotics using the disc diffusion method. A total of thirty-four Salmonella species was isolated. The percentage occurrence of Salmonella sp. in the catfishes examined was very high (80%). The incidence of Salmonella sp. in the intestine (86.7%) of the catfish was higher than for the gills (66.7%) and the skin (73.3%). Majority of the isolates were resistant to Amoxicillin, Sulfomethoxazole-trimethoprim, Amoxicillin-clavulanic acid and Streptomycin. This study therefore demonstrated the occurrence of Salmonella species in African catfish with some exhibiting antibiotic resistance. Thus, there is a potential risk of transmission of drug resistant Salmonella species to man when contaminated catfish is consumed. The use of antibiotics in fish farming should be regulated so as to decrease antibiotic residues in fish.

scholarly journals Bacteriological Quality and Antibiotics' Susceptibility Profile of Small-medium Scale Commercial Fish farms in Nigeria

2020 ◽  
Vol 14 (1) ◽  
pp. 198-208
Author(s):  
Cecilia Nireti Fakorede ◽  
Evelyn Nwadinkpa Fatokun ◽  
Blessing Philip-Kantiok ◽  
Chinwe Juliana Iwu ◽  
Ishmael Festus Jaja
Keyword(s):  
Water Samples ◽  
Heterotrophic Bacteria ◽  
Resistance Index ◽  
Fish Pond ◽  
Coliform Bacteria ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Fish Farms ◽  
Commercial Fish ◽  
Bacteriological Quality

Background: Fish currently provide 6.7% of all proteins consumed by humans globally; nevertheless, the aquaculture system has been linked to fish, environmental contamination and disease outbreak. The aim of this study was to determine the bacteriological quality and the antibiotic resistance profile of bacteria from water samples of pond stocked with Tilapia and Catfish in Ile-Ife, Osun State, Nigeria. Objective: To isolate, identify and characterise heterotrophic bacteria and test for the antibiogram of detected Coliforms. Methods: Water samples were collected from ponds stocked with Tilapia and Catfish, and tested for total heterotrophic and coliform bacteria as well as the antibiogram. The susceptibility of the isolates was tested using the Kirby-Bauer disc diffusion method on Mueller Hinton agar. Results: A total of 40 isolates were recovered from the water samples, of which 5 species were Gram positive bacteria representing two genera, and 35 species were Gram negative bacteria representing four genera. The temperature for all ponds ranged from 25°C to 28°C. The mean bacterial count varied from 1.9×104 to 5.4×104 CFU/ml per fish pond. All isolates were 100% resistant to ceftazidime, cefuroxime and augmentin. More resistance to cefixime (80%) and gentamicin (73.3%) and nitrofurantoin (66.7%) was also recorded. However, only 16.6% and 8.3% of the isolates were resistant to ciprofloxacin and ofloxacin, respectively. The multiple antimicrobial resistance index (MARI) ranged from 0.5 to 0.9. The water physicochemical parameters (temperature and pH) and the type of bacteria detected in all pond types did not differ significantly. Conclusion: Fish pond is a reservoir of multi-drug resistant bacteria that could serve as environmental source of drug resistance gene transfer. This calls for effective monitoring and assessment as well as management devices for the protection of community and environmental health.

PREVENTIVE MEASURES IN BREEDING FISHING FARMS: ECOLOGICAL AND VETERINARY ADDITIONS

2018 ◽  
Vol 1 (1) ◽  
pp. 84-89
Author(s):  
A. M. Naumova ◽  
◽  
L. A. Rozumnaya ◽  
A. Yu. Naumova ◽  
L. S. Loginov ◽  
...  
Keyword(s):  
Preventive Measures ◽  
Fish Farming ◽  
Fish Health ◽  
Fish Farms ◽  
Scientific Publications ◽  
Commercial Fish ◽  
Fish Products ◽  
Contagious Diseases ◽  
Veterinary Research ◽  
Significant Damage

The influence of ecological, epizootological and technological factors on fish health was studied. It is shown that disregard of environmental, technological and veterinary-sanitary requirements in the reproduction and cultivation of fish leads to the emergence of contagious and non-contagious diseases and causes significant damage to fish farming. The analysis of previously approved veterinary and sanitary rules, instructions, recommendations for breeding and commercial fish farms, as well as patent documentation and scientific publications in the field of ecological and veterinary research in fish farming is carried out. The possibility of using modern ecological and veterinary-sanitary achievements for the protection of fish health is shown. The complex system of preventive measures (organizational, environmental-technological and veterinary-sanitary), supplemented by modern ecological and veterinary achievements, in its implementation will allow to reduce losses of fish products and increase efficiency of production.

scholarly journals Enzymes as Biomarkers of Environmental Stress in African Catfish (Clarias gariepinus) in Osun State, Nigeria

2017 ◽  
Vol 7 (14) ◽  
pp. 71-83
Author(s):  
Omolara Titilayo Aladesanmi ◽  
Femi Kayode Agboola ◽  
Rapheal Emuebe Okonji
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Anthropogenic Activities ◽  
Clarias Gariepinus ◽  
Fish Farming ◽  
Fish Tissue ◽  
Enzymatic Activities ◽  
African Catfish ◽  
Fish Ponds ◽  
Bioindicator Species

Background. Many natural aquatic bodies have been contaminated with heavy metals released from domestic, industrial and other anthropogenic activities. Fish are an important bioindicator species and play an important role in the monitoring of water pollution. Objectives. This study shows the effect of heavy metals on the distribution of glutathione S-transferases (GST), catalase, rhodanese and 3-mercaptopyruvate sulphur transferase (3-MST) isolated from the liver, gills, fins and muscle of Clarias gariepinus. Methods . Glutathione S-transferase, catalase, rhodanese and 3-mercaptopyruvate S-transferase enzymes were isolated from the liver and gills of fish by homogenization of each tissue (with specific buffers for each enzyme) and centrifugation. Serial dilutions of the crude enzymes were then assayed for residual enzymatic activities using standard enzyme assay protocol. Results. The results showed heavy metals in the liver and muscle of the investigated fish. This study indicated significant accumulation of heavy metals in the tissues/organ of the fish from Ilesha, Osogbo and Yakoyo fish ponds. These are three main towns in Osun State where the major occupation is fish farming. The relationship between enzymatic activities and heavy metal content in C gariepinus tissue showed positive and significant (p<0.05) correlations between lead (Pb) and GST as well as chromium (Cr) and GST. This implies that higher concentrations of Pb and Cr induced the expression of greater GST activity in the fish tissue. Conclusions. The study concluded that the pattern of response of GST, catalase, rhodanese and 3-MST activities in the various organs/tissues of C gariepinus to the heavy metals suggests that the excitation or inhibitions of their activities are organ specific. Further biochemical studies of fish tissues/organs are needed to characterize the enzymatic changes associated with heavy metal pollution. Competing Interests: The authors declare no competing financial interests

scholarly journals Effect Of Commercial Biofertilizer Product Of Seaweed (Euchema Cottonii) Towards The Growth Of Pakcoy Mustard (Brassica Rapa L) and Dumbo Catfish (Clarias Gariepinus) in Aquaponic System

2020 ◽  
Vol 9 (2) ◽  
pp. 86
Author(s):  
Oktaviana Elsa Dewi ◽  
Sapto Andriyono ◽  
Mochammad Amin Alamsjah
Keyword(s):  
Survival Rate ◽  
Clarias Gariepinus ◽  
Fish Farming ◽  
African Catfish ◽  
Optimal Dosage ◽  
Vegetable Production ◽  
Randomized Design ◽  
Growing Medium ◽  
Completely Randomized Design ◽  
Biomass Plant

Aquaponics is a technology that combines the cultivation of vegetable production and fish farming. The aquaponics technology also to fertilization to give nutrients to the plant to support of survival. In facts, fertilization done to plants is located on land. Fertilizing the plants that live in the waters are still very rare, because the waters as a growing medium that is considered to always provide sufficient nutrients for plant growth. In this research, using biofertilizer Euchema cottonii of seaweed. The advantages of biofertilizer seaweed is to have the content of macro and micro nutrients are complete. It also contains substances hyper Grow (PGR) plant (auxin, cytokinin, gibberellin, abisat acid and ethylene).In this research to determine the effect of biofertilizer products of seaweed (E. cottonii) commercial on the growth of dumbo catfish (C. gariepinus) and pakcoy mustard (B. rapa L) on aquaponics system. The design of experiments in this research using a completely randomized design (CRD) with 5 treatments and 4 replications. The treatment used is fertilizer "AB mix®" 1% (A), without fertilizer (B) and the provision of products biofertilizer seaweed (E. cottonii) commercial 0,16% (C), 0,18% (D) and 0,20% (E). The main parameters measured were heavy growth of biomass, plant height pakcoy mustard (B. rapa L), growth SGR, length and survival rate of fish of dumbo catfish (C. gariepinus).The results showed that the dose of different commercial product biofertilizer seaweed (E. cottonii) gives a significantly different effect on the height growth of pakcoy mustard (B. rapa L) and the survival rate of dumbo catfish (C. gariepinus). The optimal dosage of commercial seaweed (E. cottonii) biofertilizer product in increasing the growth of pakcoy mustard (B. rapa L.) and African catfish (C. gariepinus) in the aquaponic system, namely treatment E at a dose of 0.20%.

scholarly journals IDENTIFIKASI ZIGOSITAS IKAN LELE (Clarias gariepinus) TRANSGENIK F-2 YANG MEMBAWA GEN HORMON (PhGH) DENGAN MENGGUNAKAN METODE REALTIME-qPCR

2016 ◽  
Vol 11 (1) ◽  
pp. 39
Author(s):  
Huria Marnis ◽  
Bambang Iswanto ◽  
Romy Suprapto ◽  
Imron Imron ◽  
Raden Roro Sri Pudji Sinarni Dewi
Keyword(s):  
Real Time ◽  
Clarias Gariepinus ◽  
Fish Farming ◽  
Progeny Test ◽  
African Catfish ◽  
Mendelian Segregation ◽  
Qpcr Method ◽  
Pcr Method ◽  
Real Time Qpcr ◽  
Transgene Transmission

Produktivitas ikan budidaya dapat ditingkatkan melalui teknologi transgenesis. Populasi ikan lele transgenik cepat tumbuh telah dihasilkan dan karakter biologisnya telah diketahui. Namun informasi zigositas ikan lele transgenik perlu ditelaah lebih lanjut. Penelitian ini bertujuan untuk mengidentifikasi zigositas ikan lele transgenik F-2. Zigositas ikan lele transgenik diidentifikasi dengan menggunakan metode real-time qPCR (RT-qPCR) dan uji progeni. Identifikasi zigositas melalui uji progeni, dilakukan dengan mendeteksi transgen (PhGH) pada individu-individu F-3 hasil persilangan transgenik F-2 dengan non-transgenik. Hasil penelitian menunjukkan bahwa zigositas pada ikan lele transgenik F-2 dapat diidentifikasi dengan menggunakan metode RT-qPCR. Semua ikan transgenik F-2 adalah heterozigot, dengan nilai 2-Ct yang hampir sama tiap individu F-2, yaitu berkisar 0,80-0,99. Identifikasi zigositas dengan metode RT-qPCR menunjukkan hasil yang sama dengan uji progeni, semua transgenik F-2 tidak menghasilkan 100% anakan F-3 positif transgen. Pada uji progeni, transmisi transgen pada penelitian ini tidak mengikuti hukum segregasi Mendel, dengan kisaran sebesar 5%-40%.Fish farming productivity can be increased by transgenesis technology. On the previous study, transgenic African catfish population fast growing has been produced and its biological characters has been known. However information of transgenic zygosity of catfish should be examined. The aim of this study was to identify the zygosity of F-2 transgenic African catfish. The zygosity of F-2 transgenic was identified by real time-qPCR (RT-qPCR) method and progeny test. Further, identification of zygosity F-2 transgenic African catfish was confirmed by progeny test, while F-2 transgenic African catfish was mated with non-transgenic. Identification of zygosity F-2 transgenic was conducted by detection PhGH gene (transgene) in F-3 transgenic African catfish population. Transgene transmission was evaluated by PCR method. The result showed that the zygosity F-2 transgenic African catfish could be identified by RT-qPCR method. All F-2 transgenic African catfish were heterozygous, where as the 2-Ct value was almost same for all individual, which ranges from 0.80 to 0.99. The result of zygosity identification using RT-qPCR method was as same as that of progeny test. In the progeny test, transgene transmission in this study was non-Mendelian segregation, with ranges of 5%-40%.

scholarly journals FISHING IN DRAINING PONDS OF MODERN FISH FARMS

2020 ◽  
Vol 2020 (4) ◽  
pp. 131-137
Author(s):  
Dmitriy Alekseevich Kostrykin ◽  
Yulia Nikolaevna Grozesku
Keyword(s):  
Mechanical Damage ◽  
Fish Farming ◽  
Fish Ponds ◽  
Fish Farms ◽  
Farmed Fish ◽  
Severe Injuries ◽  
Commercial Fish ◽  
Technological Stage ◽  
Time Frames ◽  
Excessive Accumulation

The article describeы the fishing process as a final technological stage that completes the cycle of fish breeding in the fish ponds. Fishing in the ponds, regardless of their category, is a difficult and energy-intensive process. The safety of farmed fish and the overall results of fish farming depend on the process of fishing. The well-known fishing methods are based on filtering fish when discharging water through fish catchers or other artificial structures. When water is drained from the pond, the fish moves to a fish catcher with the water flow, which contradicts its biological tendency to breast the current and prevents from its getting into the fish catcher or another artificial structure. In addition, the separation of fish from water by a grated water separator leads to the mechanical damage of the fish scales and reduces the number of alive fish. The technology of fish catching in draining ponds used at the small innovative enterprise SRK Sharapovsky (Kamyzyaksky district, Astrakhan region) has been analyzed. The absence of a fish catcher is a specific feature of the technology. Fishing in the ponds is carried out by using a dragnet during the pond drainage, starting the catch when the pond’s area is lowered to 1/3 or 1/4. The disadvantages of this technology include excessive accumulation of fish in a small volume of water, which can cause fish death or severe injuries to the underyearlings reared with commercial fish species. Choosing the optimal fishing gear or a device will allow to clearly plan and coordinate the work of the enterprise, because the fishing period is short and depends on the time frames and the market demands.

scholarly journals Antibacterial Activity of Isolates from Digestive Tract of Catfish (Clarias gariepinus) As Probiotic Candidates At PT. Biotech Cipta Kreasi

2021 ◽  
Vol 3 (1) ◽  
pp. 138-149
Author(s):  
Toto Apriliyanto ◽  
Nosa Septiana Anindita ◽  
Wisnu Adhi Susila
Keyword(s):  
Digestive Tract ◽  
Aeromonas Hydrophila ◽  
Low Cost ◽  
Clarias Gariepinus ◽  
Fish Farming ◽  
African Catfish ◽  
Bacillus Sp ◽  
High Level ◽  
Control Management ◽  
Probiotic Activity

African catfish (Clarias gariepinus) is a popular fish in the community because it provides a high level of protein at a low cost. The motile aeromonas septicemia (MAS) disease produced by Aeromonas hydrophila (A. hydrophila) infection is one of the things that create difficulties in the cultivation of this fish. Synthetic antibiotics are commonly used in MAS disease control management efforts in fish farming, which have have an influence not only on fish but also on consumers. The application of probiotic Bacillus sp isolated from the digestive tract of catfish as a means of preventing the sickness is one option. Enzymatic tests (lipolytic, amylolytic, cellulolytic, proteolytic activity) and antagonist testing were used to assess the probiotic activity of the Bacillus sp isolates.

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