scholarly journals Mari Mutare

2021 ◽  
pp. 92-105
Author(s):  
Vanessa Lorenzo Toquero
Keyword(s):  
Design Research ◽  
Human Subjects ◽  
Petri Dish ◽  
Physical Reality ◽  
Self Reflection ◽  
Early Christian ◽  
Gender Culture ◽  
Human Exceptionalism ◽  
Plant Hybrid ◽  
Speculative Design

Mari mutare is a transdisciplinary design research project about biocompatible prostheses inspired by the early Christian being called Green Man, a human-plant hybrid that represents the nature-culture continuum. These objects are intended to address human exceptionalism from a post-anthropocentric, feminist and queer perspective. The aim of Mari mutare is to explore the multiplicity of subjectivities in ourselves and, consequently, to influence the perception of others. Arising from the emerging field of synthetic biology, speculative design methodology supports this proposal and it materialises through transhackfeminist biopractices as tools for creating knowledge and projecting other possible futures. The experiments are conducted around the Petri dish as an epistemic object. The dish contains a symbiotic assembly of human and plant cells that interpenetrate, digest and partially assimilate while grazing the categories of kingdom, species, gender, culture and nature. While this process materialises, human subjects test their future limbs aided by an augmented reality (AR) filter, as a proxy for the physical reality, to hack into self-reflection and subjectivity, thus projecting themselves beyond the self. This project is currently a work-in-progress and is supported by Pro Helvetia, Hangar Barcelona (EU Biofriction programme), Utopiana Geneva and Hackuarium.

scholarly journals Envisioning Design Futures via Practice-led Speculative Design Research

2016 ◽  
Vol 9 (3) ◽  
Author(s):  
Brad Tober
Keyword(s):  
Design Research ◽  
Future Research ◽  
Maker Movement ◽  
Essential Component ◽  
Commercial Potential ◽  
True Value ◽  
Production Technologies ◽  
Speculative Design ◽  
Pure Research ◽  
The Maker Movement

Some criticisms of the contemporary maker movement (referring to the democratization of various production technologies) have conflated the validity of the making arising from this movement with the utility / commercial potential of said making. However, like pure research in traditional disciplines, the true value of exploratory making—of which practice-led research is a part—without immediate utility or application is in the development of a base for future research to build upon. This paper both establishes a context and proposes a framework for practice-led speculative design research, which positions the designer as an instigator whose work can inform that of both other designers and non-designers (such as those engaged in the maker movement). Such work holds the power to chart the course and ensure the future of the discipline as a whole, and thus should be viewed as an essential component of disciplinary activity. 

scholarly journals Edible speculations: designing everyday oracles for food futures

2021 ◽  
Author(s):  
Markéta Dolejšová
Keyword(s):  
Design Research ◽  
Research Project ◽  
Contested Space ◽  
Food Practices ◽  
Food Cultures ◽  
Food Tech ◽  
Speculative Design

Digital food technologies carry promise for better food futures but they are often problematic in their impact on food cultures. While proponents suggest that food-tech products such as smart kitchenware or diet personalisation services can support efficient food practices, critics highlight various risks. This paper presents our findings from Edible Speculations, a long-term design research project exploring the contested space of food-tech innovation through a series of speculative design (SD) events situated in everyday public contexts. We illustrate the opportunities and limits of eventful SD in supporting critical engagements with food-tech issues through an Edible Speculations case study called the Parlour of Food Futures. Our discussion of selected Parlour events can inform readers interested in food-tech themes as well as those keen on experimenting with eventful approaches to SD research.

Effective purpose in transnational humanitarian healthcare providers

2013 ◽  
Vol 8 (3) ◽  
pp. 157-168 ◽  
Author(s):  
Donna J. Perry, PhD, RN
Keyword(s):  
Sample Size ◽  
Medical Center ◽  
Human Subjects ◽  
Academic Medical Center ◽  
Healthcare Providers ◽  
Snowball Sampling ◽  
Educational Preparation ◽  
Self Reflection ◽  
Academic Medical ◽  
Making A Difference

Objective: To advance knowledge regarding the education and support needs of staff deployed to international settings from a US academic medical center (AMC).Design: A qualitative approach rooted in phenomenology called, Transcendental Method for Research with Human Subjects was used. A flexible interview guide was used to guide participants into self-reflection about the decision to participate in global healthcare, educational preparation, field experiences, and return.Setting: The study was conducted at a US AMC.Participants: Sample size was 15 and included nurses, physicians, and therapists who had participated in disaster and/or developmental humanitarian global health deployments. Purposive sampling with a maximum variation approach was used along with snowball sampling. Sample size was determined by reaching horizonal understanding of participants.Main outcome measures: The study sought to elicit and analyze responses from participants in an open-ended manner.Results: Analysis revealed the following seven themes: a) the yearning to relieve suffering, b) getting ready, c) making a difference, d) bad things happening to wonderful people, e) challenging and sustaining factors, f) dialectical alienation, and g) knowing what really matters. The concept of “effective purpose” emerged from interpretation of these themes.Conclusions: Most participants found their experiences to be beneficial and meaningful but faced challenges in the field. Knowledge and skills varied among providers. Education and support are critical for healthcare professionals who engage in transnational healthcare. Recommendations for staff preparation are provided.

The Phenomenology of Sigmund Freud

1993 ◽  
Vol 24 (2) ◽  
pp. 101-129 ◽  
Author(s):  
Frederick J. Wertz
Keyword(s):  
Direct Observation ◽  
Sigmund Freud ◽  
Subject Matter ◽  
Human Subjects ◽  
Phenomenological Theory ◽  
Relational Theory ◽  
Natural Attitude ◽  
Self Reflection ◽  
Theory Of Meaning ◽  
Scientific Truth

AbstractThe convergences in approach between Freud's psychoanalysis and Husserl's phenomenology are elaborated. These include philosophical roots in Brentano's teachings; the primacy of direct observation over construction and theory; a conviction about the irreducibility of mentality to nature; the project of a "pure" psychology; the bracketing of theories, preconceptions, and the natural attitude; the necessity of self-reflection and empathy; a relational theory of meaning; receptivity to human subjects as teachers; and the methodological value of fiction for scientific truth. It is argued that divergences between psychoanalytic and phenomenological theory have obscured profound agreement in the approach, subject matter, and methods of these two schools of psychology.

scholarly journals Identifying Linguistic Competence What Linguistic Competence Consists in

2016 ◽  
Vol 2 (1) ◽  
pp. 120
Author(s):  
Jesus Martinez del Castillo
Keyword(s):  
Verbal Behavior ◽  
Speech Acts ◽  
Human Subjects ◽  
Linguistic Competence ◽  
Self Reflection

<p>Linguistic competence, language, a language and speech acts constitute realities to be found in speaking. They all are nothing but aspects of the same reality, the activity of speaking created and executed by human subjects who are free and creative, absolute and contingent, transcendent and historical. Since speaking is something known by speakers even before the performance what linguistic competence is can only be guessed out through self-reflection and verification of it in the verbal behavior of speakers.</p>

Goodbye Old Friend: A Son's Farewell to Comiskey Park

1992 ◽  
Vol 25 (2) ◽  
pp. 87-93 ◽  
Author(s):  
Bob Krizek
Keyword(s):  
Social Scientist ◽  
Physical Reality ◽  
Ethnographic Research ◽  
Self Reflection ◽  
The Past ◽  
Research Activities ◽  
The Social

Comiskey Park, the home of the Chicago White Sox, closed its gates for the last time on September 30, 1990, after a glorious eighty-year reign as “the world's greatest baseball palace.” I attended that event as both a social scientist engaged in an ethnographic project and a son seeking to reconnect with the memories of and feelings for his father. In the recording and analysis of the language of those in attendance that day, the scientist within me began to recognize and understand a physical “reality” of memories and the symbolic importance of “bridges” to the past. In the writing, the ethnographer soon came to appreciate and more fully explore the reflexive properties and possibilities of ethnographic research. As a son, I confronted, in part through my research activities, a variety of emotions regarding my father, his death, and my previously unaddressed grief. The son and the social scientist each began to realize the role old Comiskey Park had played in my relationship with my father. This article, a brief but emotionally faithful piece of self-reflection, is written more by the son than the researcher. It is my farewell to my father.

The Fine Structure of Rat Granulosa Cell Cultures Correlated with Progestin Secretion

1973 ◽  
Vol 31 ◽  
pp. 552-553
Author(s):  
T. M. Crisp ◽  
F.R. Denys
Keyword(s):  
Fine Structure ◽  
Granulosa Cell ◽  
Cell Cultures ◽  
Single Injection ◽  
Surrounding Medium ◽  
Petri Dish ◽  
Female Rats ◽  
Vaginal Smear ◽  
Immature Female ◽  
Serum Gonadotropin

The purpose of this paper is to present observations on the fine structure of rat granulosa cell cultures grown in the presence of an adenohypophyseal explant and to correlate the morphology of these cells with progestin secretion. Twenty-six day old immature female rats were given a single injection of 5 IU pregnant mares serum gonadotropin (PMS) in order to obtain ovaries with large vesicular follicles. At 66 hrs. post-PMS administration (estrus indicated by vaginal smear cytology), the ovaries were removed and placed in a petri dish containing medium 199 and 100 U penicillin/streptomycin (P/S)/ml. Under a 20X magnification dissecting microscope, some 5-8 vesicular follicles/ovary were punctured and the granulosa cells were expressed into the surrounding medium. The cells were transferred to centrifuge tubes and spun down at 1000 rpm for 5 mins.

Rotary Beveling for In Situ Thin-Section Microscopy of Cell Cultures

1981 ◽  
Vol 39 ◽  
pp. 550-551
Author(s):  
Dean A. Handley ◽  
Jack T. Alexander ◽  
Shu Chien
Keyword(s):  
Cell Growth ◽  
Cell Cultures ◽  
Molecular Interactions ◽  
Convenient Method ◽  
Petri Dish ◽  
Simple Method ◽  
Thin Section Microscopy ◽  
Thin Sectioning ◽  
Organic Fluids

In situ preparation of cell cultures for ultrastructural investigations is a convenient method by which fixation, dehydration and embedment are carried out in the culture petri dish. The in situ method offers the advantage of preserving the native orientation of cell-cell interactions, junctional regions and overlapping configurations. In order to section after embedment, the petri dish is usually separated from the polymerized resin by either differential cryo-contraction or solvation in organic fluids. The remaining resin block must be re-embedded before sectioning. Although removal of the petri dish may not disrupt the native cellular geometry, it does sacrifice what is now recognized as an important characteristic of cell growth: cell-substratum molecular interactions. To preserve the topographic cell-substratum relationship, we developed a simple method of tapered rotary beveling to reduce the petri dish thickness to a dimension suitable for direct thin sectioning.

Unlabeled Antibody Immunocytochemistry Applied to Murine Mammary Tumor Cells

1975 ◽  
Vol 33 ◽  
pp. 390-391
Author(s):  
Wm. J. Arnold ◽  
J. Russo ◽  
H. D. Soule ◽  
M. A. Rich
Keyword(s):  
Horseradish Peroxidase ◽  
Mammary Tumor ◽  
Covalent Binding ◽  
Petri Dish ◽  
Gamma Chain ◽  
Mammary Tumor Virus ◽  
Mammary Tumor Cells ◽  
Murine Mammary Tumor ◽  
Tumor Virus ◽  
Unlabeled Antibody

Our studies of mammary tumor virus have included the application of the unlabeled antibody enzyme method of Sternberger to mammary tumor derived mouse cells in culture and observation with an electron microscope. The method avoids the extravagance of covalent binding of indicator molecules (horseradish peroxidase) with precious antibody locator molecules by relying instead upon specific antibody-antigen linkages. Our reagents included: Primary Antibody, rabbit anti-murine mammary tumor virus (MuMTV) which was antiserum 113 AV-2; Secondary Antibody, goat anti-rabbit IgG gamma chain (Cappel Laboratories); andthe Indicator, rabbit anti-horseradish peroxidase - horseradish peroxidase complex (PAP) (Cappel Labs.). Dilutions and washes were made in 0.05 M Tris 0.15 M saline buffered to pH 7.4. Cell monolayers, after light fixation in glutaraldehyde, were incubated in place by a protocol adapted from Sternberger and Graham and Karnovsky, then embedded by our usual method for monolayers. Reagents were confined to specific areas by neoprene 0-rings (Parker Seal Co.) reducing the amount of reagent needed to 50 microliters, 1/6th of that required to wet a 35 mm petri dish.

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