scholarly journals Uji Aktivitas Antibakteri Ekstrak Kasar Bawang Putih (Allium sativum ) terhadap Bakteri Shigella dysenteriae yang Resisten terhadap Ampisilin

MEDULA ◽  
2019 ◽  
Vol 6 (2) ◽  
Author(s):  
Arimaswati Arimaswati ◽  
Yessy Toding Mendaun ◽  
Yenti Purnamasari
Keyword(s):  
Antibacterial Activity ◽  
Allium Sativum ◽  
Research Result ◽  
Organosulfur Compound ◽  
Negative Control ◽  
Shigella Dysenteriae ◽  
Control Group ◽  
Clear Zone ◽  
Gastrointestinal Infections ◽  
Control Group Design

ABSTRACT Background : Gastrointestinal infections, especially caused by Shigella dysenteriae bacteria is still a health issue in Indonesia. The number of ampicillin resistance to S. dysenteriae has been widely reported. Garlic (Allium sativum) is a traditional medicine that efficacy as an antibacterial. Garlic contains an organosulfur compound  Allicin. Research Purpose: This study aimed to determine the antibacterial activity of garlic rule extract on the growth of S. dysenteriae bacteria that are resistant to ampicillin. Research Methods: This study used the experimental with post test only control group design. The antibacterial activity test was determined based on the diameter of clear zone formed. Test  were performed  on garlic filtrate, ciprofloxacin as a positive control and aquades as a negative control. Each treatment was performed three times. Research Result: This research showed that rule extract of garlic (A. sativum)  had a clear zone against S. dysenteriae that was resistant to ampicillin. Conclusion: The conclusion of this research was that rule extract of garlic (A. sativum) had an antibacterial activity against S. dysenteriae which resistant to ampicillin.  Keywords : Antibacterial activity, Garlic (A. sativum), S. dysenteriae

scholarly journals Antibacterial Activity of Curcuma longa (turmeric), Curcuma zedoaria (zedoary), and Allium sativum (garlic) Nanoparticle Extract on Chicken with Chronic Respiratory Disease Complex: In Vivo Study

2020 ◽  
Vol 151 ◽  
pp. 01054
Author(s):  
Ekowati Handharyani ◽  
Lina N. Sutardi ◽  
Aulia A. Mustika ◽  
Andriani Andriani ◽  
Sri Yuliani
Keyword(s):  
Antibacterial Activity ◽  
Respiratory Disease ◽  
Allium Sativum ◽  
Curcuma Longa ◽  
Clinical Signs ◽  
Chronic Respiratory Disease ◽  
Negative Control ◽  
Control Group

Previous in vitro studies showed that nanoparticle extract of turmeric, zedoary, and garlic exhibit antibacterial activity against Mycoplasma gallisepticum (M. gallisepticum) which causes chronic respiratory disease (CRD) in chicken. This research aimed to determine the antibacterial activity of nanoparticles of Curcuma longa, Curcuma zedoaria, and Allium sativum extract to CRD infected chicken. In vivo test of antibacterial activity of turmeric, zedoary, and garlic nanoparticle in combination was conducted on chicken infected by M. gallisepticum and Escherichia coli (E.coli). Antibiotic control used was enrofloxacin. As many as 75 chickens were divided into 5 groups containing 15 chickens each. Group one consisted of healthy chickens (positive control); group two consisted of chickens that have been inoculated by bacteria (negative control); group three (treatment) were chickens inoculated by bacterium and given extract nanoparticle combination on day 7 of infection for 7 days; group four (prevention) were chickens inoculated by bacterium and given combination of extract nanoparticles on day 5 before infection for 14 days; group five were chickens inoculated with bacterium and given enrofloxacin antibiotics for 7 days. In vivo research results showed increased body weight and performance indicated by improvements in clinical signs, and gross pathology changes. The combination of three extract nanoparticles showed the best activity in controlling CRD in chicken, both as preventive and curative means.

scholarly journals Uji in vitro Aktivitas Antibakteri Minyak Atsiri Batang Sereh (Cymbopogon citratus) terhadap Shigella dysenteriae

2018 ◽  
Vol 6 (2) ◽  
pp. 262
Author(s):  
Yunita Wulansari ◽  
Enny Suswati ◽  
Septa Surya Wahyudi
Keyword(s):  
Antibacterial Activity ◽  
Alternative Therapy ◽  
Tween 80 ◽  
Inhibition Zone ◽  
Negative Control ◽  
Shigella Dysenteriae ◽  
Diffusion Method ◽  
Control Group ◽  
High Morbidity

  Shigella dysenteriae is bacteria that caused diarrhea with high morbidity and mortality. Resistance and toxicity has reported in some antibiotics therapy towards S. dysentriae. Therefore, alternative therapy based on herbal plants is needed. Chymbopogon citratus oil can be used as an antibacterial to Escherichia coli, Klebsiella pneumoniae, Streptococcus mutans, all strains Staphlylococcus sp, Bacillus cereus and Bacillus subtilis. This study aimed to investigate antibacterial activity of C. citratus oils on the growth of S. dysentriae and its minimum inhibitory concentration (MIC). This type of research was a quasi experimental. Antibacterial activity test performed by diffusion method. Subjects in this study were S. dysentriae. C. citratus oil concentration used were 15 μl/ml, 20 μl/ml, 25 μl/ml, 30 μl/ml, 35 μl/ml and 40 μl/ml. Positive control group was given ciprofloxacin 5 μl/ml and a negative control group was given tween-80. Inhibition zone was observed at a concentration of 25 µl/ml (P3). The MIC of C. citratus oils againts S. dysentriae was at a concentration of 25 µl/ml. Kruskal Wallis analysis showed that p=0.000 and α=0.05, it was revealed that there were differences between antibacterial activity amoung tested group with different concentration. Keywords: Shigella dysenteriae, Chymbopogon citratus oil, antibacterial  

The Effectiveness Of Anchovy Concentration (Stolephorus insularis) as Antimicrobial to Streptococcus mutans (In Vitro)

DENTA ◽  
2016 ◽  
Vol 10 (1) ◽  
pp. 71
Author(s):  
Almira Fa’Izah ◽  
Istien Wardani ◽  
Diana Soesilo
Keyword(s):  
Dental Caries ◽  
Streptococcus Mutans ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Control Group ◽  
Oral Diseases ◽  
Clear Zone ◽  
Control Group Design

<p><strong><em>Background:</em></strong><em> Dental and oral diseases which </em><em>a</em><em>re often found in children is dental caries. Streptococcus mutans </em><em>i</em><em>s the main cause of caries. Caries c</em><em>an</em><em> be prevented by using a topical application of fluoride. The Anchovy (Stolephorus insularis) contain</em><em>s</em><em> protein, vitamins (A, B<sub>1</sub>, C), and minerals (Fe, Ca, K, F). Calcium fluoride (CaF<sub>2</sub>) within the anchovy c</em><em>an</em><em> inhibit the occurrence of dental caries. <strong>Purpose</strong></em><strong><em>: </em></strong><em>The aim of this study was </em><em>to </em><em>determine the </em><em>antimicrobial </em><em> ability of anchovy extract (Stolephorus insularis) to Streptococcus mutans. <strong>Materials and</strong> <strong>Methods: </strong>This study was a laboratory experimental research </em><em>with</em><em>  post test only control group design. Diffusion method were applied with 2 controls: negative control used DMSO 1%, positive control used NaF solution, and 3 concentrations of anchovy extract (Stolephorus insularis) 3%, 6%, and 12%, each group were composed of 6 samples. </em><em>Antimicrobial</em><em> was assessed by measuring the diameter of the clear zone around the discs contained the anchovy extract (Stolephorus insularis). Data were analyzed by Kruskal-Wallis test followed by Mann-Whitney test.<strong> Result:</strong> The results from this study showed clear zone around the discs of the anchovy extract (Stolephorus insularis). The more concentration of the extract showed the more </em><em>antimirobial</em><em> zone diameter. The average zone of </em><em>antimicrobial</em><em> at  </em><em>the </em><em>concentration of  3% </em><em>were </em><em>7,11 mm, 6%</em><em> </em><em>9,5 mm, 12%</em><em> </em><em>10,78 mm, for the negative control DMSO 1% 6 mm and the positive control NaF solution 8,16 mm. The largest diameter of the clear zone was at concentration of 12% (P &lt; 0,05).</em><em> </em><strong><em>Conclusion: </em></strong><em>The<strong> </strong>anchovy extract (Stolephorus insularis) had an antimicrobial effect to the growth of Streptococcus mutans.</em><em></em></p>

scholarly journals The Difference in Antibacterial Potency of the Infusion of Akar Kuning (Fibraurea tinctoria Lour.) against Shigella dysenteria and Salmonella typhi In Vitro

2021 ◽  
Vol 17 (2) ◽  
pp. 103
Author(s):  
Mohammad Bakhriansyah ◽  
Divae Sandrainy ◽  
Agung Biworo
Keyword(s):  
Antibacterial Properties ◽  
Inhibition Zone ◽  
Salmonella Typhi ◽  
Negative Control ◽  
Test Method ◽  
Shigella Dysenteriae ◽  
Control Group ◽  
Control Group Design ◽  
The Difference

Abstract: Akar kuning (Fibraurea tinctoria Lour.), an original plant from Kalimantan, is often used by the community as traditional medicine. Previous studies showed that akar kuning contains active compounds such as alkaloids, flavonoids, saponins, and terpenoids that have antibacterial properties. This study aims to analyze the difference in antibacterial potency of the infusion of akar kuning against the growth of Shigella dysenteriae (S. dysenteriae) and Salmonella typhi (S. typhi) in vitro. This was a true experimental study with a post-test method only with control group design using the infusion of akar kuning with concentrations of 16%, 32%, and 64%, 5 µg ciprofloxacin as the positive control and aquadest as the negative control. The data were analyzed using the One-Way ANOVA test, LSD’s Post-hoc test, and independent T test with a 95% of confidence level. This study showed that the higher the concentration of the infusion of akar kuning, the larger the inhibition zone of the infusion at the concentration of 64% had a larger inhibition zone for S. typhi (16,32 mm) than S. dysenteriae (15,59 mm). However, there was no statistical difference in antibacterial potency of  the infusion of akar kuning against S. dysenteriae and S. typhi.  Keywords: antibacterial potential, infusion, akar kuning, Fibraurea tinctoria Lour., Shigella dysenteriae, Salmonella typhi

scholarly journals PENGARUH PEMBERIAN EKSTRAK BAWANG PUTIH (Allium sativum Linn) TERHADAP ZONA HAMBAT PERTUMBUHAN BAKTERI Staphylococcus epidermidis secara in vitro

2019 ◽  
Vol 20 (1) ◽  
pp. 28-31
Author(s):  
AKBAR DIOSASQIA PUTRA
Keyword(s):  
Staphylococcus Epidermidis ◽  
Allium Sativum ◽  
Antibacterial Effect ◽  
Negative Control ◽  
Garlic Extract ◽  
Diffusion Method ◽  
Control Group ◽  
High Morbidity ◽  
Control Group Design

Background:Infectious diseases are the main cause of high morbidity and mortality rates, especially in developing countries such as Indonesia.Indonesia is abundant with plants that can be used as herbal medicine. Garlic is known as having antibacterial elements, one of them is for Staphylococcus epidermidis  which is one cause of dermal disease. This disease is often ignored in Indonesia because the price of the drug is not affordable by the community. So that garlic should be used as an alternative medicineObjective: This study aims to determine the antibacterial effect of garlic extract (Allium sativum L.) retarding the growth of Staphylococcus epidermidis.Methods: This study is experimental laboratory using plate diffusion method of "Postest Ony Control Group Design" design. There are 7 groups, 5 treatment groups and 2 control groups. The concentration of treatment group was 100%, 50%, 25%, 12,5%, 6,25% while for positive control using chloramphenicol and aquadest as negative control. The bacteria used were Staphylococcus epidermidis.Results: The results showed that garlic extract (Allium sativum L.) had an antibacterial effect in inhibiting the growth of Staphylococcus epidermidis. The inhibitory zone concentration in the statistical results shows there are significant differences with values p = 0.000 or p <α.Conclusion: The conclusion of the study showed that Garlic Extract (Allium sativum L.) had an antibacterial effect to retarding the growth of Staphylococcus epidermidis..Keywords: Staphylococcus epidermidis, garlic, antibacteria

Pengaruh Model PjBL Terhadap Hasil Belajar Murid pada Mata Pelajaran Akidah Akhlak di Madrasah Ibtidaiyah

2020 ◽  
Vol 8 (01) ◽  
pp. 110-126
Author(s):  
Nashran Azizan ◽  
Rahmadani Tanjung
Keyword(s):  
Research Result ◽  
Control Group ◽  
Research Model ◽  
Control Group Design ◽  
Islamic School ◽  
Group Design ◽  
Quasi Experimental ◽  
Moral Lesson ◽  
Learning Test ◽  
Academic Year

This aimed to know the influence of PjBL model toward learning results of moral lessons in knowing the prophet and apostle in IV grade of Islamic School Padangsidimpuan Angkola Julu in academic year 2019/2020 by using PjBL and Direct Instruction model. The research model used Quasi-Experimental Design with None Quivalent Control-Group Design. The used instrument is the learning result test. Data analysis used t-Test. Research result showed (1) there is the influence of PjBL model toward student's learning test on moral lesson of knowing prophet and apostle material.

TOKSISITAS AKUT INFUSA KULIT ARI KACANG TANAH (Arachis hypogea L.) PADA MENCIT BALB/ C

2018 ◽  
Vol 15 (2) ◽  
pp. 62
Author(s):  
Risha Fillah Fithria ◽  
Ririn Lispita Wulandari ◽  
Devi Nisa Hidayati ◽  
Lilis Rejeki
Keyword(s):  
Single Dose ◽  
Color Change ◽  
Negative Control ◽  
Control Group ◽  
Hair Color ◽  
Peanut Shell ◽  
Control Group Design ◽  
Safety Standards ◽  
Arachis Hypogea ◽  
Passive Behavior

ABSTRACTPeanut shell (PS) infusion has been shown to be antithrombocytopenia, but there has been no research on safety standards. This study aims to identify the symptoms of toxic effects, the potency of toxicity and histopathology of liver male Balb/C mice after a single dose of PS infusion. This research uses randomized matched posttest only control group design. Twenty five mice were divided into 5 orally dosage groups, ie, PS infusion with a dose of 0,026; 0.052; 0.104; 0.208 g/20gBW; and negative control of CMC Na 0.5%. The observation period is for 14 days. The results showed that single dose of PS infusion had a pseudo LD50 value ie > 0.208g/20gBW which was practically non toxic. Symptoms to watch out for the BW infusion were passive behavior, bradycnea, hair color change, hair loss, and weight loss at doses of 3 and 4. It is unclear whether liver damage ie inflammation, necrosis, and albuminous degeneration caused by PS infusion or other causes.keywords: acute toxicity, infusion, peanut shell

scholarly journals Sequestration of erythrocytes infected with Plasmodium berghei ANKA in BALB/c mice treated with goat bile

2020 ◽  
Vol 4 (2) ◽  
pp. 179
Author(s):  
Kartika Arum Wardani ◽  
Kholida Nur Aini ◽  
Heny Arwati ◽  
Willy Sandhika
Keyword(s):  
Plasmodium Berghei ◽  
Antimalarial Activity ◽  
Negative Control ◽  
Control Group ◽  
Dependent Manner ◽  
Plasmodium Berghei Anka ◽  
Concentration Dependent Manner ◽  
Control Group Design ◽  
Bile Concentration

Abstract Sequestration of Plasmodium berghei ANKA-infected erythrocytes occurs in BALB/c mice as characteristic of  Plasmodium falciparum infection in humans. Animals’ bile has been widely used for centuries in Traditional Chinese Medicine. Goat bile has been used in healing infectious and non-infectious diseases; however, no report on the use of goat bile against malaria infection and sequestration. The purpose of this study was to analyze the correlation between parasitemia and sequestration in the liver of P.berghei ANKA-infected BALB/c mice treated with goat bile. This research was an in vivo experimental study using the post-test control group design. The male BALB/c mice aged ± 6 weeks, body weight 20-25 g were used. The mice were divided into five groups where Group 1-3 were mice treated with goat bile 25%, 50%, and 100%, respectively. Group 4-5 were negative (sterile water) and positive controls (DHP). Parasitemia was observed daily from each mouse and the number of sequestered infected erythrocytes on the endothelium of sinusoids. The data were analyzed using t independent test. Antimalarial activity of goat bile was shown by the lower parasitemia in goat bile-treated mice compared with the negative control. The average number of sequestration was goat bile concentration-dependent manner. The higher the concentration, the lower the number of sequestration. Sequestration was correlated with parasitemia (p=0,0001). Sequestration of P.berghei ANKA-infected erythrocytes correlated with parasitemia, and was goat bile concentration-dependent manner. Keywords: Malaria, parasitemia, sequestration, goat bileCorrespondence: [email protected]

scholarly journals Pengaruh Pemberian Ekstrak Teripang Pasir terhadap Hambatan Pertumbuhan Shigella Dysentriae Secara In Vitro

2020 ◽  
Vol 18 (1) ◽  
pp. 47
Author(s):  
FERIZAL NEGERI SAMUDRA ◽  
RETNO BUDIARTI ◽  
IRMAWATI IRMAWATI
Keyword(s):  
Sea Cucumber ◽  
Antibacterial Effect ◽  
Positive Control ◽  
Negative Control ◽  
Shigella Dysenteriae ◽  
Control Group ◽  
Control Groups ◽  
Shigella Spp ◽  
Diarrhea Disease

<p><strong>ABSTRACT</strong></p><p><strong>Background</strong>; In Indonesia, most diarrhea disease in 1995 to 2001 are caused by Shigella spp. Shigella spp infection can cause various symptom dan complication. Generally, the treatment by using antibiotic can cause antibiotic resistance. Sea cucumber (Holoturia scabra) is an herb that known, available, and easy to consume by society and has an antibacterial effect. Therefore, further research to study the effect of Holoturia Scabra on <em>Shigella Dysentriae</em> growth in vitro is needed.</p><p><strong>Objectives</strong>: The goal of this research is demonstrate the effect of sea cucumber (Holoturia scabra) to the growth of the <em>Shigella dysentriae</em> bacteria in vitro.</p><p><strong>Method</strong>: The method in this research is Posttest Only Control Group. There are 6 groups, 4 types of and 2 control groups. The concentration of the treatment group is 100%,50%, 25%, and, 12.5% while for positive control tests using chloramphenicol and aquadest as a negative control.</p><p><strong>Result</strong>: The result showed there is an influence on the intake of sand cucumber to the growth of the Shigella dysenteriae.</p><p><strong>Conclusion</strong>: Sea cucumber (<em>Holoturia scabra</em>) inhibit the growth of <em>Shigella dysenteriae</em>.</p><p><strong>Key words</strong>: <em>Shigella dysenteriae</em>, sea cucumber (<em>Holoturia scabra</em>), antibacterial</p>

scholarly journals PENGARUH EKSTRAK DAGING LIDAH BUAYA (Aloe Vera) TERHADAP PENYEMBUHAN ULSERASI MUKOSA MULUT PADA MALE WISTAR RATS

2015 ◽  
Vol 1 (1) ◽  
pp. 25
Author(s):  
Laila Fitrotuz Zahroh ◽  
Rahmawati Sri Praptiningsih ◽  
Moh. Baehaqi
Keyword(s):  
Oral Mucosa ◽  
Wistar Rats ◽  
Healing Process ◽  
Research Result ◽  
Aloe Vera ◽  
Control Group ◽  
Control Group Design ◽  
Significant Difference ◽  
Male Wistar Rats

Background: Oral mucosa ulceration which often occurs usually in the form of white-yellowish spot with concave surface, reddish edge and pain. Based on previous research, Aloe vera process anti-inflammation substance that could help quickening ulceration healing process. This research aims to know the effect of Aloe vera flesh extract on Male wistar rats oral mucosa ulceration in-vivo. Method: this research was quasi experimental research with the post-test only control group design using Male wistar rats as the testing animal. In the research, there were three treatment groups: The first groups which was given aquadest treatment, second groups with Aloe vera flesh extract, and third groups which was given chlorhexidine gluconate 0,2% treatment. The data collecting was based on histopathology observation concerning the increase of fibroblast quantity. Result: The research result based on comparison test among the three groups with One Way Anova showed that on Day 3th, the average quantity of fibroblast didn't have significant difference between the treatment group and control group positive that was p>0,05, meanwhile on Day 7th every group showed significant difference p<0,05. Conclusion: It concluded that Aloe vera flesh extract has influence on the healing of Male wistar rats oral mucosa ulceration as shown by fibroblast increasing quantity.

Sign in / Sign up

Export Citation Format

Share Document