scholarly journals Prevalence of Bovine Mastitis in the Western Region of Cameroon

2021 ◽  
pp. 1-5
Author(s):  
Müller FOTSAC DZOUSSE ◽  
◽  
Marc K. KOUAM ◽  
Keyword(s):  
Bovine Mastitis ◽  
Clinical Signs ◽  
Subclinical Mastitis ◽  
Clinical Mastitis ◽  
Feeding Method ◽  
Lactating Cows ◽  
Stage Of Lactation ◽  
California Mastitis Test ◽  
Farm Type ◽  
Age Range

Mastitis is the major disease in milking cows causing huge losses to the dairy industry worldwide. It is in view to contribute to the eradication of this pathology that the present study was carried out in cattle farms of the West Region of Cameroon from March 2020 to November 2020. A total of 473 lactating cows of local and improved breeds were screened for mastitis. Screening for clinical and sub-clinical mastitis was based on the observation of clinical signs and on the use of the California Mastitis Test (CMT) respectively. The overall prevalence was 34.88%, among which 9.72% and 25.16% represented clinical and subclinical mastitis respectively. In summary, the factors influencing the occurrence of mastitis were: the divisions surveyed (p<0.001), breed type (p=0.003), age range (p<0,001), farm type (p=0.05), soil type (p<0,001), cleaning frequency (p<0.001), feeding method (p<0.001), hygiene of udder (p=0.05) and stage of lactation (p=0.02). The results obtained allow us to recommend an improvement of the breeding conditions to prevent mastitis, because once mastitis appears in a farm, it becomes very difficult to eradicate it.

scholarly journals Epidemiology of Bovine Mastitis and Associated Risk Factors in East Shewa Zone of Oromia Regional State, Ethiopia

2017 ◽  
Vol 2 (2) ◽  
pp. 1-6
Author(s):  
Sadia Hajie
Keyword(s):  
Risk Factors ◽  
Clinical Examination ◽  
Bovine Mastitis ◽  
Clinical Mastitis ◽  
Cross Sectional Study ◽  
Cross Sectional ◽  
Lactating Cows ◽  
Stage Of Lactation ◽  
California Mastitis Test ◽  
Smallholder Dairy

A cross sectional study was conducted in selected districts of East Shewa Zone of Oromia Regional Stat from November 2013 to April 2014 on dairy cows to determine the prevalence of mastitis and determinant of its major risk factors. The study was done on 250 lactating cows, of which 109 were local and 14 1 cross breed by using clinical examination , California mastitis test (CMT) and culture. Of these 108 (43.2 %) were positive by clinical examination and CMT for clinical and sub clinical mastitis, with prevalence of 4 % and 38.8 %, respectively. All the p otential risk factors considered in this study namely, parity ( p < 0.05), breed (p< 0.05), age (p < 0.05), stage of lactation (p < 0.05),breed (p < 0.05) and husbandry (p < 0.05) showed a significant effect on prevalence of mastitis in present study. Thus , high prevalencence of mastitis was observed in older cows >7 years and cows with parity >7 but high prevalence was obtained on early and late stage of lactation. The study also revealed that cross breed lactating cows and those managed intensively were s usceptible to mastitis. The highly prevalent bacteria isolated were Staphylococcus aurous (46 %) followed by Streptococcus agalactiae which accounts (19.4 %), and others were Staphylococcus intermidius, E. coli and Corynebacterial species with respective pr oportion of 16.6, 10.9 and 7.1%. The present study also revealed that mastitis is the major problem in smallholder dairy farms in the study area specially the sub clinical form, so that creation of awareness about the importance and prevention of subclinic al mastitis among smallholder dairy farmers, milking infected animals and periodic monitoring of infection status of the udder is recommended.

Variations in the levels of acute-phase proteins and lactoferrin in serum and milk during bovine subclinical mastitis

2021 ◽  
pp. 1-5
Author(s):  
Aarsha Raj ◽  
Vinodkumar Kulangara ◽  
Tresamol P. Vareed ◽  
Deepa P. Melepat ◽  
Latha Chattothayil ◽  
...  
Keyword(s):  
Acute Phase ◽  
Acute Phase Proteins ◽  
Solid Phase ◽  
Bovine Mastitis ◽  
Subclinical Mastitis ◽  
Clinical Mastitis ◽  
Reactive Protein ◽  
Amyloid A ◽  
California Mastitis Test ◽  
Bacterial Organism

Abstract Variations in the levels of acute phase proteins and lactoferrin in serum and milk for diagnosis of subclinical mastitis in dairy cows are described in this research paper. Milking animals from two organized dairy farms in Kerala, India, were screened by California Mastitis Test (CMT), Electrical Conductivity test (EC) and Somatic Cell Count (SCC) test to identify animals affected with sub clinical mastitis (SCM). The concentrations of acute phase proteins (APP) Haptoglobin (Hp), C- reactive protein (CRP), Albumin, Lactoferrin (Lf) and α- 1 acid glycoprotein (AGP) in milk and Hp, Albumin, Serum Amyloid A (SAA) and CRP in the serum of 40 normal cows and 40 cows affected with sub clinical mastitis were assessed. Solid phase ELISA was employed for assessment of all parameters except the albumin levels, for which spectrophotometry was used. The values of Hp in milk; and SAA, AGP and Lf in serum, were significantly elevated in the group with sub clinical mastitis. Such variations were found to be independent of the specific bacterial organism causing the disease. These results show that significant variations exist in the levels of acute phase proteins Hp, AGP and Lf in milk, and SAA in serum of animals affected with subclinical bovine mastitis that are not affected by specific bacterial etiology.

scholarly journals Molecular Detection and Sensitivity to Antibiotics and Bacteriocins of Pathogens Isolated from Bovine Mastitis in Family Dairy Herds of Central Mexico

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Ma. Fabiola León-Galván ◽  
José E. Barboza-Corona ◽  
A. Arianna Lechuga-Arana ◽  
Mauricio Valencia-Posadas ◽  
Daniel D. Aguayo ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Bovine Mastitis ◽  
Dairy Herd ◽  
Subclinical Mastitis ◽  
Herd Size ◽  
Clinical Mastitis ◽  
Dairy Herds ◽  
Coagulase Negative Staphylococci ◽  
California Mastitis Test ◽  
Streptococcus Uberis

Thirty-two farms (n=535cows) located in the state of Guanajuato, Mexico, were sampled. Pathogens from bovine subclinical mastitis (SCM) and clinical mastitis (CLM) were identified by 16S rDNA and the sensitivity to both antibiotics and bacteriocins ofBacillus thuringiensiswas tested. Forty-six milk samples were selected for their positive California Mastitis Test (CMT) (≥3) and any abnormality in the udder or milk. The frequency of SCM and CLM was 39.1% and 9.3%, respectively. Averages for test day milk yield (MY), lactation number (LN), herd size (HS), and number of days in milk (DM) were 20.6 kg, 2.8 lactations, 16.7 animals, and 164.1 days, respectively. MY was dependent on dairy herd (DH), LN, HS, and DMP<0.01, and correlations between udder quarters from the CMT were around 0.49P<0.01. Coagulase-negative staphylococci were mainly identified, as well asStaphylococcus aureus,Streptococcus uberis,Brevibacterium stationis,B. conglomeratum, andStaphylococcus agnetis. Bacterial isolates were resistant to penicillin, clindamycin, ampicillin, and cefotaxime. Bacteriocins synthesized byBacillus thuringiensisinhibited the growth of multiantibiotic resistance bacteria such asS. agnetis,S. equorum,Streptococcus uberis,Brevibacterium stationis, andBrachybacterium conglomeratum, but they were not active againstS. sciuri, a microorganism that showed an 84% resistance to antibiotics tested in this study.

Quarter-wise prevalence of subclinical mastitis in crossbred cows

2017 ◽  
Author(s):  
Rachna Ullah ◽  
Manju Ashutosh ◽  
Sujata Pandita ◽  
Ashutosh . ◽  
Panjab Singh Yadav ◽  
...  
Keyword(s):  
Subclinical Mastitis ◽  
Clinical Mastitis ◽  
Research Needs ◽  
Crossbred Cows ◽  
Lactating Cows ◽  
California Mastitis Test ◽  
Karan Fries

The present study was designed to determine the quarter-wise and animal-wise prevalence of sub-clinical mastitis in crossbred Karan Fries (Holstein x Tharparker) lactating cows. For this purpose, the udders of 73 cows were screened by modified California Mastitis Test (mCMT) and EC measurements. The efficiency of detection of mastitis and non-mastitis by EC measurements was 86% and 93% respectively. Animal-wise prevalence was found 41%, while quarter-wise prevalence was 31.16%. Among mastitic quarters, prevalence of single quarter infection, two quarter infection, three quarter infection and all four quarter infection per animal, was 0.00, 26.66, 43.33 and 30.00%, respectively. Out of all quarters, maximum prevalence was found in right hind quarters (29.67%) followed by left hind (26.37%), left fore (23.07%) and right fore (20.87%).The same pattern was observed during EC measurements. Though EC showed similarity with CMT in the detection of subclinical mastitis, the research needs to be carried out on more number of animals.

scholarly journals PREVALENCE OF SUB-CLINICAL MASTITIS AT BANARIPARA UPAZILLA, BARISAL

2017 ◽  
Vol 15 (1) ◽  
pp. 21-26 ◽  
Author(s):  
D. Biswas ◽  
T. Sarker
Keyword(s):  
Risk Factors ◽  
Clinical Mastitis ◽  
Physiological Status ◽  
Educational Effort ◽  
Lactating Cows ◽  
California Mastitis Test ◽  
Farm Type ◽  
Substantial Problem ◽  
Test Kit ◽  
Baseline Information

A study was aimed to determine the prevalence of sub-clinical mastitis and also determine the other risk factors that intensify this condition. Prescribed questionnaire was used to take baseline information of the animals and farms and California mastitis test kit was used to determine the SCM in lactating cow at farm level. It appears from this study that an overall prevalence of SCM was 51.56% in milking cows at Banaripara Upazilla, Barisal. Crossbred cows were significantly affected with SCM than local breed lactating cows. The farm type affect significantly (p<0.05) on the occurrence of this diseases. The prevalence of sub-clinical mastitis in cow was significantly (p<0.05) higher in 3rd (80%) parity compared to 1st (38.09%) and 2rd (45.83%) parity as well as non pregnant cows (55.55%) are more prone to infection than pregnant cow (46.43%). The farm floor condition and aged cows don’t have any effect on SCM. Prevalence of sub-clinical mastitis was significantly (p<0.05) higher in high yielding (87.5%) cows than medium (70%) to low (33.33%) yielding cows. A well documented continued research and educational effort is required to increase producer awareness of cost due to mastitis to the dairy enterprise. From this study it was concluded that cow breed, farm type, parity of cows, physiological status of animal and milk yield per cow were found to be important risk factors associated with the occurrence of sub clinical mastitis in dairy cows. The occurrence of this disease remains a substantial problem for dairy producers.

scholarly journals Mastitogenic bacteria isolated from dairy cows in Kenya and their antimicrobial sensitivity

2014 ◽  
Vol 85 (1) ◽  
Author(s):  
George K. Gitau ◽  
Royford M. Bundi ◽  
John Vanleeuwen ◽  
Charles M. Mulei
Keyword(s):  
Dairy Cattle ◽  
Dairy Cows ◽  
Bovine Mastitis ◽  
Antibiotic Sensitivity ◽  
Subclinical Mastitis ◽  
Clinical Mastitis ◽  
California Mastitis Test ◽  
Antimicrobial Sensitivity ◽  
Significant Difference ◽  
Causative Agents

There is limited epidemiological knowledge on udder health in Kenyan dairy cattle that would aid in a pro-active approach towards mastitis prevention. The study objectives were: (1) to investigate the prevalence and distribution of clinical and subclinical mastitis in dairy cattle in Mukurwe-ini and Nakuru Districts, Kenya, and (2) to determine the antibacterial sensitivity of the organisms causing bovine mastitis in these districts. The study involved field-screening of milk samples from 241 dairy cows on 128 farms by use of the California Mastitis Test (CMT) and, if CMT-positive, followed by bacteriological culture of the major causative agents and their respective antibiotic sensitivity to eight commonly used antibiotics. All participating farms were visited twice during the study period. The results obtained during the first and second visits showed the prevalence of clinical mastitis to be very low: 0.9% and 0.5%, respectively; 56.0% and 65.0% of cows were CMT-positive on at least one quarter and 49.6% and 58.7% of cows were culture-positive, respectively. There was no significant difference in mastitis prevalence between Nakuru and Mukurwe-ini districts (p > 0.10). Staphylococcus aureus was isolated in 68.0% and 77.0% of samples during the first and second visits, respectively. Other frequently isolated agents included Streptococcus agalactiae, and other Streptococcus spp., S. aureus and S. agalactiae were most sensitive to gentamycin and norfloxacin, and least sensitive to cotrimazole and ampicillin. Knowing the prevalence of mastitogenic organisms and their antibiotic sensitivities could improve treatment efficacy and cow longevity.

scholarly journals Prevalence of sub-clinical mastitis in dairy farms

2014 ◽  
Vol 30 (2) ◽  
pp. 70-77
Author(s):  
MA AL Quaderi ◽  
M Husain ◽  
MGS Alam ◽  
M Khatun ◽  
MA Hossain
Keyword(s):  
Prevalence Rate ◽  
Dairy Farm ◽  
Dairy Farms ◽  
Subclinical Mastitis ◽  
Clinical Mastitis ◽  
Cattle Breeding ◽  
Lactating Cows ◽  
California Mastitis Test ◽  
Breeding Station ◽  
High Prevalence

A total of 560 lactating cows belonging to Bangladesh Agricultural University dairy farm (n = 59): Local Zebu (L), Local × Friesian (L × F), Local × Jersey (L × J), Local × Red Chittagong Cattle (L × RCC), Local × Sahiwal (L × SL), Red Chittagong Cattle (RCC); and Central Cattle Breeding Station and Dairy Farm (n = 501): Local Zebu, L × F, SL × F, Sahiwal and Australian Friesian Sahiwal (AFS) were selected to measure prevalence of subclinical mastitis (SM). The California Mastitis Test was done and the prevalence of SM was 68% on cow basis and 57% on quarter basis. The prevalence rate was significantly higher in L × F (87%, P < 0.05), SL × F (88%, P < 0.05), L × J (100%, P < 0.01), AFS (89%, P< 0.05) and SL (100%, P < 0.01). The local Zebu (31%) and RCC (28%) were least susceptible to SM. SM was significantly higher in multiparous (P < 0.05) and older cows (P < 0.05). The front quarters were more prone to SM than the rear. Factors such as breed, age, parity and management may have been responsible for high prevalence of SM in both farms. DOI: http://dx.doi.org/10.3329/bvet.v30i2.18257 Bangl. vet. 2013. Vol. 30, No. 2, 70-77

scholarly journals Microbiome Dynamics of Bovine Mastitis Progression and Genomic Determinants

2020 ◽  
Author(s):  
M. Nazmul Hoque ◽  
Arif Istiaq ◽  
M. Shaminur Rahman ◽  
M. Rafiul Islam ◽  
Azraf Anwar ◽  
...  
Keyword(s):  
Bacterial Colonization ◽  
Bovine Mastitis ◽  
Antibiotic Resistance Genes ◽  
Subclinical Mastitis ◽  
Clinical Mastitis ◽  
Bacterial Strains ◽  
Microbiome Composition ◽  
Viral Genomes ◽  
Lactating Cows ◽  
Metagenome Sequencing

AbstractThe milk of lactating cows presents a complex ecosystem of interconnected microbial communities which can impose a significant influence on the pathophysiology of mastitis. Previously, we reported the alteration of microbiome (bacteria, archaea, virus) composition between clinical mastitis (CM) and healthy (H) milk. We hypothesized possible dynamic shifts of microbiome compositions with the progress of different pathological states of mastitis (CM, Recurrent CM; RCM, Subclinical Mastitis; SCM) determined by its favoring genomic potentials. To evaluate this hypothesis, we employed whole metagenome sequencing (WMS) in 20 milk samples (CM = 5, RCM = 6, SCM = 4, H = 5) to unravel the microbiome dynamics, interrelation, and relevant metabolic functions. PathoScope (PS) and MG-RAST (MR) analyses mapped the WMS data to 442 bacterial, 58 archaeal and 48 viral genomes with distinct variation in microbiome composition and abundances across these metagenomes (CM>H>RCM>SCM). PS analysis identified 385, 65, 80 and 144 bacterial strains in CM, RCM, SCM, and H milk, respectively, with an inclusion of 67.19% previously unreported opportunistic strains in mastitis metagenomes. Moreover, MR detected 56, 13, 9 and 46 archaeal, and 40, 24, 11 and 37 viral genera in CM, RCM, SCM and H-milk metagenomes, respectively. The CM-microbiomes had closest association with RCM-microbiomes followed by SCM, and H-microbiomes. Furthermore, we identified 333, 304, 183 and 50 virulence factors-associated genes (VFGs), and 48, 31, 11 and 6 antibiotic resistance genes (AGRs) in CM, RCM, SCM, and H-microbiomes, respectively, showing a significant correlation between the relative abundances of VFGs (p = 0.001), ARGs (p = 0.0001), and associated bacterial taxa. We also detected correlated variations in the presence and abundance of several metabolic functional genes related to bacterial colonization, proliferation, chemotaxis, motility and invasion, oxidative stress, virulence and pathogenicity, phage integration and excision, biofilm-formation, and quorum-sensing to be associated with different episodes of mastitis. Therefore, profiling the dynamics of microbiome in different states of mastitis, concurrent VFGs, ARGs, and genomic functional correlations will contribute to developing microbiome-based diagnostics and therapeutics for bovine mastitis, and carries significant implications on curtailing the economic fallout from this disease.

scholarly journals HOST DETERMINANTS BASED COMPARATIVE PREVALENCE OF SUBCLINICAL MASTITIS IN LACTATING HOLSTEIN-FRIESIAN CROSS COWS AND RED CHITTAGONG COWS IN BANGLADESH

1970 ◽  
Vol 8 (1) ◽  
pp. 17-21 ◽  
Author(s):  
AFMG Rabbani ◽  
MA Samad
Keyword(s):  
Milk Production ◽  
Dairy Farm ◽  
Subclinical Mastitis ◽  
Clinical Mastitis ◽  
Severe Reaction ◽  
Lactating Cows ◽  
Host Determinants ◽  
California Mastitis Test ◽  
Lactation Stage ◽  
Holstein Friesian

A comparative prevalence of sub-clinical mastitis (SCM) based on host risk factors was studied by using California Mastitis Test (CMT) in 96 Holstein-Friesian cross cows (HFCC) of Abu Baker Dairy Farm at Pansha Upazilla in Rajbari district and 20 Red Chittagong cows (RCC) of Bangladesh Agricultural University Dairy Farm (BAUDF), Mymensingh during the period from January to June 2008. Out of the 116 lactating cows examined, 51 (43.97%) showed positive reactivity to CMT. Similar prevalence rate of SCM was recorded in both the HFCC (43.75%) and RCC (45.0%). The overall prevalence of SCM on the basis of lactation stages showed that all the three lactation stages had SCM but there was a tendency to increase its prevalence rates from early (30.43%), mid (32.26%) to late (69.23%) stages. The highest prevalence of SCM was recorded at late lactation stage in both the HFCC (68.75%) and RCC (71.43%) in comparison to their respective mid and early lactation stages. The parity-wise prevalence of SCM was recorded as 18.42%, 55.0%, 66.67%, 75.0% and 75.0% in HFCC whereas, it was 20.0%, 20.0%, 66.67%, 75.0% and 66.67% in RCC at their 1st, 2nd, 3rd, 4th and 5th parity, respectively. This study recorded a relationship between the increased prevalence of SCM with decreased of milk production in both the groups of lactating cows. The respective prevalence of SCM at high, medium and low level of milk production in HFC (14.29%, 33.33% and 70.59%) and RCC (14.29%, 50.00% and 71.42%) were found significantly (p < 0.01) correlated with milk production. An overall 19.18% quarters was affected with SCM, of which 4.31%, 7.33% and 7.38% showed mild, moderate and severe reaction to CMT. However, comparatively, higher percentage of quarter infection was recorded in HFCC (20.31%) than RCC (13.75%). It may be concluded from these observations that both the HFCC and RCC should be monitored by using CMT for the detection of SCM in order to provide rational treatment and control it to make the dairy farming profitable. DOI = 10.3329/bjvm.v8i1.7397 Bangl. J. Vet. Med. (2010). 8(1): 17-21

scholarly journals Etiology of Mastitis and Antimicrobial Resistance in Dairy Cattle Farms in the Western Part of Romania

Antibiotics ◽  
2022 ◽  
Vol 11 (1) ◽  
pp. 57
Author(s):  
Corina Pascu ◽  
Viorel Herman ◽  
Ionica Iancu ◽  
Luminita Costinar
Keyword(s):  
Antimicrobial Resistance ◽  
Bovine Mastitis ◽  
Rapid Test ◽  
Subclinical Mastitis ◽  
Multidrug Resistant ◽  
Clinical Mastitis ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
California Mastitis Test ◽  
Staphylococcus Spp

The present study aimed to determine the bacteria isolated from bovine mastitis and their antimicrobial resistance in the western part of Romania. Clinical mastitis was diagnosed based on local inflammation in the udder, changes in milk, and when present, generalized symptoms. Subclinical mastitis was assessed using a rapid test—the California Mastitis Test. The identification of bacterial strains was performed based on biochemical profiles using API system tests (API 20 E, API Staph, API 20 Strep, API Coryne, API 20 NE (bioMerieux, Marcy l’Etoile, France), and MALDI-TOF mass spectrometry (MS). The prevalent isolated bacteria were Staphylococcus spp. (50/116; 43.19%), followed by Streptococcus spp. (26/116; 22.41%), E. coli (16/116; 13.79%), Corynebacterium spp. (9/116; 7.75%), Enterococcus spp. (10/116; 8.62%), and Enterobacter spp. (5/116; 4.31%). Phenotype antimicrobial resistance profiling was performed used the disc diffusion method. Generally, Gram-positive bacteria showed low susceptibility to most of the antimicrobials tested, except cephalothin. Susceptibilities to penicillins and quinolones were fairly high in Gram-negative bacteria, whereas resistance was observed to macrolides, aminoglycosides, and tetracyclines. The highest number of isolates were multidrug resistant (MDR), the resistance pathotypes identified including the most frequently antimicrobials used in cow mastitis treatment in Romania.

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