scholarly journals Antibiotic Sensitivity Pattern and Plasmid Profile of Bacteria Isolated from Diabetic Ulcers in Mbano Metropolis, Imo State, Southeastern Nigeria

2021 ◽  
Vol 6 (1) ◽  
pp. 38-46
Author(s):  
E.O Nwankwo ◽  
◽  
E. E. Nwagbara ◽  
K.N. Onusiriuka
Keyword(s):  
Escherichia Coli ◽  
Plasmid Profile ◽  
Klebsiella Pneumonia ◽  
Diabetic Patients ◽  
Southeastern Nigeria ◽  
Alkaline Lysis ◽  
Diabetic Ulcers ◽  
E Coli ◽  
Microbiological Methods ◽  
Sensitivity Pattern

The study was undertaken to evaluate the bacteriology and antibiogram of isolates from diabetic patients with chronic foot ulcers in Nigeria. A total of 150 pus samples were collected and processed according to standard aerobic and anaerobic microbiological methods. Antibiogram was done using Kirby-Bauer method. Biofilm tests, ESBL & AmpC production was conducted using Congo red agar, Double disc synergy test and Cefoxitin disc test respectively. Total number of isolates obtained was 210. The Plasmid profiles of some of the Multi-Drug Resistance (MDR) isolates were carried out using the alkaline lysis method for plasmid extraction and electrophoresis on agarose gel with standard markers. The most frequently isolated aerobic organism in the study was Escherichia coli (32.1%) while the least occurring was Enterobacter spp (1.57%). For the anaerobes, Peptostreptococcus spp (40%) was the highest isolated bacterium. Percentage of Extended Spectrum -lactamase ( ESBL) producers among E. coli isolates was 44%. Percentages of biofilm formation potential among the isolates were: E. coli (36.8%), S. aureus (23.1%) and Proteus vulgaris (4.2%). Escherichia coli and S. aureus showed considerable levels of resistance to some common antibiotics. No methicilin resistant S. aureus was encountered. AmpC producers encountered were Klebsiella pneumonia (10%) and E. coli (8.1%). Post-curring antibiogram tests revealed that nine isolates carried plasmids, suggesting that the mode of resistance may be plasmid mediated. Keywords: Diabetic ulcers, Bacteria, Antibiogram, Plasmid profile

Drug Sensitivity Pattern of E. Coli Causing Urinary Tract Infection in Diabetic and Non-Diabetic Patients

1996 ◽  
Vol 24 (3) ◽  
pp. 296-301 ◽  
Author(s):  
F Jinnah ◽  
M S Islam ◽  
M A K Rumi ◽  
M G Morshed ◽  
F Huq
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Drug Sensitivity ◽  
Diabetic Patients ◽  
Remarkable Difference ◽  
E Coli ◽  
Sensitivity Pattern ◽  
Tract Infections

Escherichia coli was isolated from 65 urine samples out of 300 specimens collected from diabetic and non-diabetic patients with urinary tract infections. All isolates were screened for susceptibility to commonly used antibiotics. No remarkable difference in sensitivity pattern was observed between the E. coli isolates from diabetic and non-diabetic out-patients. However, the isolates from hospitalized diabetic patients showed a clear difference in sensitivity; E. coli from this group was more resistant to commonly used antibiotics compared with those from non-hospitalized diabetic and non-diabetic patients.

scholarly journals Antimicrobial Susceptibility Pattern of Klebsiella pneumonia and Escherichia coli Isolated from Stool Samples of Patients in Two Tertiary Hospitals in Rivers State, Nigeria

2020 ◽  
pp. 44-49
Author(s):  
S. I. Douglas ◽  
N. P. Akani ◽  
N. C. Kamani
Keyword(s):  
Escherichia Coli ◽  
Public Health Problem ◽  
Multi Drug Resistance ◽  
Klebsiella Pneumonia ◽  
Susceptibility Pattern ◽  
E Coli ◽  
Microbiological Methods ◽  
Tertiary Hospitals ◽  
Stool Samples ◽  
Rivers State

Reduced susceptibility of antibiotics against Enterobacterial strains have emerged as an important public health problem worldwide. Infections caused by Escherichia coli and Klebsiella pneumonia can affect severely ill patients, and their colonization of human gut, endangers population at large in communities, and in hospitals. This research is aimed at determining the susceptibility pattern of Escherichia coli and Klebsiella pneumonia from stool of patients in two tertiary hospitals in Rivers State, Nigeria. A total of 114 stool samples were collected from patients. Stool samples were collected in sterile biological specimen bottles and were sent to the laboratory immediately after collection. Stool samples were inoculated by streaking on Eosin methylene blue and MacConkey agar plates. Isolates were characterized using standard microbiological methods and were stored and used for further tests. The result showed that nineteen isolates of E. coli were 100% resistant to Cefuroxime and Augmentin, while 78.9%, 68.4% and 42.1% were resistant to cefixime, Ceftazidime and Nitrofurantoin, respectively. The result for the susceptibility pattern of the Klebsiella isolates showed 100% resistance to cefuroxime and Augmentin. Resistance to ceftazidime, cefixime and nitrofurantoin were observed to be 70%, 60% and 45%, respectively. Isolates of E. coli and Klebsiella were highly susceptible to Meropenem and ofloxacin. The isolates of E. coli and Klebsiella showed multi-drug resistance to the different antibiotics. Although the meropenem, ofloxacin and ciprofloxacin antibiotics showed high level of sensitivity to these isolates, there were still some level of resistance recorded.

scholarly journals Antibiotic Susceptibility and Genotype Patterns of Escherichia coli, Klebsiella pneumonia and Pseudomonas aeruginosa Isolated from Urinary Tract Infected Patients

2010 ◽  
Vol 59 (3) ◽  
pp. 207-212 ◽  
Author(s):  
M.I. ABOU-DOBARA ◽  
M.A. DEYAB ◽  
E.M. ELSAWY ◽  
H.H. MOHAMED
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Urinary Tract ◽  
Antibiotic Susceptibility ◽  
Susceptibility Testing ◽  
Random Amplified Polymorphic Dna ◽  
Test Methods ◽  
Klebsiella Pneumonia ◽  
E Coli ◽  
Rapd Pcr

Thirty nine isolates of Escherichia coli, twenty two isolates of Klebsiella pneumoniae and sixteen isolates of Pseudomonas aeruginosa isolated from urinary tract infected patients were analyzed by antimicrobial susceptibility typing and random amplified polymorphic DNA (RAPD)-PCR. Antibiotic susceptibility testing was carried out by microdilution and E Test methods. From the antibiotic susceptibility, ten patterns were recorded (four for E. coli, three for K. pneumoniae and three for P. aeruginosa respectively). Furthermore, genotyping showed seventeen RAPD patterns (seven for E. coli, five for K. pneumoniae and five for P. aeruginosa respectively). In this study, differentiation of strains of E. coli, K. pneumoniae and P. aeruginosa from nosocomial infection was possible with the use of RAPD.

scholarly journals A novel report of colistin-resistant Escherichia coli carrying mcr-1 gene from animal and human feacal samples in Nigeria

2018 ◽  
Vol 1 (1) ◽  
pp. 7-10 ◽  
Author(s):  
Olugbenga A. Olowe ◽  
Rita A. Olowe ◽  
Adeolu S. Oluremi ◽  
Olusolabomi J. Adefioye
Keyword(s):  
Escherichia Coli ◽  
Animal Husbandry ◽  
Multiple Drug ◽  
Colistin Resistance ◽  
Pcr Assay ◽  
Southwestern Nigeria ◽  
E Coli ◽  
Multiple Drug Resistant ◽  
Microbiological Methods ◽  
Faecal Samples

Background: The mobilized colistin resistance (m cr)-1 gene confers transferable colistin resistance. Reports of mcr-1-positive Escherichia coli (MCRPE) have attracted substantial attention. However, in Nigeria, there is no report of mcr-1 gene resistance. Since colistin is a last resort for multiple drug-resistant isolates, this study therefore report the prevalence of mcr-1 gene among E. coli isolated from human and animal sources. Methods: Out of a total of 280 samples collected from animal and hum an faecal samples from selected farms in Oyo and Osun States, Southwestern Nigeria between July 2015 and June 2016, 60 E. coli were identified using standard microbiological methods. The mcr-1 gene was detected in the isolates by conventional PCR assay. Results: The m cr-1 gene was low and not statistically significant (p≥0.05). It was detected in 5 (8.3%) of 60 E. coli isolates (4= animals; 1= human) Conclusion: This study is the first report of mcr -1 gene from E. coli from human and animal sources in Nigeria. This calls for urgent caution in the use of colistin in animal husbandry.

scholarly journals In Vitro Antimicrobial Sensitivity of Escherichia coli Isolated from Clinical Sources

1970 ◽  
Vol 34 (1) ◽  
pp. 99-101
Author(s):  
Mohammad Shahriar ◽  
Mahboob Hossain ◽  
Shaila Kabir
Keyword(s):  
Escherichia Coli ◽  
Nalidixic Acid ◽  
E Coli ◽  
Antimicrobial Sensitivity ◽  
Sensitivity Pattern ◽  
Low Sensitivity ◽  
Academy Of Sciences ◽  
Higher Sensitivity

A study of antimicrobial sensitivity of Escherichia coli (E. coli) isolated from clinical sourcesof different diagnostic centers of Dhaka, Bangladesh was carried out to facilitate the choice of drugin the management of E. coli induced symptoms. Very low sensitivity of E. coli towards ampicillin(4%), aztreonam (4%), cloxacillin (5%), nalidixic acid (5%), ciprofloxacin (7.5%), ceftriaxone(12.5%), doxycycline (12.5%), ceftazidime (16.25%), co-trimoxazole (20%), chloramphenicol(22.51%), tetracycline (25%), and netilmicin (35%) was observed. Higher sensitivity pattern wasobserved for gentamicin (56%) and only imipenem (95%) showed sensitivity pattern possiblysusceptible enough to consider for the management of E. coli induced cases in the area under study.The low sensitivity to different antimicrobial could be attributed to their prevailing usage and abusein the area under study.Key words: E. coli; Antimicrobial sensitivity; Clinical isolatesDOI: 10.3329/jbas.v34i1.5497Journal of Bangladesh Academy of Sciences, Vol.34, No.1, 99-101, 2010

scholarly journals Whole-Genome Draft Sequences of Nine Asymptomatic Escherichia coli Bacteriuria Isolates from Diabetic Patients

2018 ◽  
Vol 6 (2) ◽  
Author(s):  
Christoph Stork ◽  
Beáta Kovács ◽  
Eva Trost ◽  
Tamás Kovács ◽  
György Schneider ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Asymptomatic Bacteriuria ◽  
Diabetic Patients ◽  
Whole Genome ◽  
Genome Sequences ◽  
Disease Response ◽  
Symptomatic Urinary Tract Infection ◽  
E Coli ◽  
Genome Draft

ABSTRACT Escherichia coli can colonize the urinary bladder without causing a disease response in the host. This asymptomatic bacteriuria (ABU) can protect against recurrent symptomatic urinary tract infection by virulent bacteria. Here, we report the whole-genome sequences of nine E. coli ABU isolates from diabetic patients.

scholarly journals Characterization and Antibiogram of Escherichia coli Associated with Mortality in Broilers and Ducklings in Bangladesh

1970 ◽  
Vol 2 (1) ◽  
pp. 9-14 ◽  
Author(s):  
MT Islam ◽  
MA Islam ◽  
MA Samad ◽  
SML Kabir
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Sensitivity ◽  
High Rate ◽  
E Coli ◽  
Sensitivity Pattern ◽  
Highly Sensitive ◽  
Biochemical Studies ◽  
Increasing Trend ◽  
Escherichia Coli Infection ◽  
Hemorrhagic Enteritis

Mortality in broilers (6.56%) and growing ducks (11.0%) caused by Escherichia coli was recorded in the experimental flocks study during the period from May to August 2003. E. coli organisms isolated from broiler birds affected with characteristic lesions of omphalitis and yolk sac infection, fibrinous pericarditis and peri-hepatitis , hemorrhagic enteritis, and accumulation of excessive pericardial and peritoneal fluid, whereas from ducks with lesions of hemorrhagic enteritis and extensive epicardial hemorrhages. Each of the 21 isolates collected from broilers and 11 isolates from ducks was characterized by cultural and biochemical studies, of which 8 isolates from broilers and 5 isolates from ducks were tested for antibiotic sensitivity with 9 different antibiotics. The antibiotic sensitivity pattern showed that the isolates were highly sensitive to ciprofloxacin but an increasing trend of resistance was recorded in broilers (7 / 9) than duck (4 / 9) isolates. It may be concluded from the results of this study that the high rate of E. coli infection in broilers and ducks along with the high resistance of isolates to antibiotics constitute a threat to the poultry industry in Bangladesh. Key words: Escherichia coli infection; mortality; broilers; ducks; characterization; antibiogram doi: 10.3329/bjvm.v2i1.1927 Bangl. J. Vet. Med. (2004). 2 (1) : 09-14

scholarly journals Bacterial Etiology and Antibiotic Sensitivity Pattern of Community Acquired Pneumonia in Diabetic Patients: Experience in a Tertiary Care Hospital in Bangladesh

2017 ◽  
Vol 7 (2) ◽  
pp. 101-105
Author(s):  
Jamal Uddin Ahmed ◽  
Mohammad Delwar Hossain ◽  
Muhammad Abdur Rahim ◽  
Farhana Afroz ◽  
AKM Musa
Keyword(s):  
Staphylococcus Aureus ◽  
Klebsiella Pneumoniae ◽  
Antibiotic Sensitivity ◽  
Community Acquired Pneumonia ◽  
Sputum Culture ◽  
Diabetic Patients ◽  
Care Hospital ◽  
E Coli ◽  
Sensitivity Pattern ◽  
Pseudomonas Species

Background: Diabetes mellitus (DM) is an immunosuppressive condition and uncontrolled diabetes is associated with increased susceptibility to various infections like pneumonia. Community acquired pneumonia (CAP) in diabetic patients is often caused by more virulent or atypical organisms and associated with increased resistance to conventional antibiotics. The aims of this study were to identify the bacterial etiology of CAP in patients with DM and to see their antibiotic sensitivity pattern.Methods: This was a cross-sectional, observational study conducted in the Department of Internal Medicine & Pulmonology of BIRDEM General Hospital, Dhaka, Bangladesh, from January 2013 to December 2015. A total of 120 hospitalized diabetic patients diagnosed with CAP and with a positive sputum culture growth of any bacteria were included in the study.Results: Majority (67%) of the patients were male. Mean age of the patients was - 55.69 ±10.5 years. Mean duration of diabetes was - 7.35 ±1.3 years. Mean HbA1c was - 8.6 ±1.89%. Sputum for culture showed that out of 120 (100%) patients, Klebsiella pneumoniae was detected in 53 (44.2%) patients, Staphylococcus aureus in 18 (15.0%), Pseudomonas species in 16 (13.3%) patients, Acinetobacter in 10 (8.3%), Escherichia coli in 9 (7.5%) patients and 14 (11.7%) patients had growth of other organisms. Sensitivity pattern of different bacterial growth in sputum to commonly used antibiotics like ceftriaxone, ciprofloxacin, amikacin and imipenem were as follows – Klebsiella (19%, 47%, 74%, 96% respectively), Staph aureus (11%, 33%, 78%, 67% respectively), Pseudomonas (19%, 75%, 81%, 88% respectively), Acinetobacter (0%, 0%, 20%, 50% respectively), E. coli (22%, 22%, 100%, 100% respectively). All (100%) of the Pseudomonas and Acinetobacter were sensitive to colistin. Most of the growth of all the bacteria (Klebsiella 94%, Staphylococcus 78%, Pseudomonas 81%, Acinetobacter 100%, E. coli 100%) occurred in patients with poor glycemic control (HbA1c ? 7.0%).Conclusion: This study results suggest that CAP in diabetic patients are more frequently due to Klebsiella pneumoniae, Staphylococcus aureus and Pseudomonas species and mostly they are less sensitive to commonly used antibiotics like ceftriaxone and ciprofloxacin. So, whenever possible, treatment of CAP should be guided by sputum culture and sensitivity test and for empirical treatment of CAP in diabetic patients, alternative antibiotics like imipenem and amikacin should be considered.Birdem Med J 2017; 7(2): 101-105

scholarly journals In-Vitro Biofilm Formation and Antimicrobial Resistance of Escherichia coli in Diabetic and Nondiabetic Patients

2019 ◽  
Vol 2019 ◽  
pp. 1-8
Author(s):  
Sunayana Raya ◽  
Ankit Belbase ◽  
Laxmi Dhakal ◽  
Krishna Govinda Prajapati ◽  
Reena Baidya ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Diabetic Patients ◽  
Microtitre Plate ◽  
Plate Method ◽  
E Coli ◽  
Biofilm Production

Background. Diabetic patients are more susceptible to urinary tract infection compared to nondiabetic patients, Escherichia coli being the most common uropathogen causing UTI. Unreasonable and incorrect antibiotic prescription for UTI in these patients may induce the development of antibiotic-resistant urinary pathogens resulting in delayed recovery and longer hospitalization. In addition to these, biofilm forming capacity of the pathogen may worsen the problem. The main aim of this cross-sectional study (conducted from March to September 2015) is to detect the biofilm forming capacity of UTI causing micro-organisms and compare the antibiotic resistance pattern of Escherichia coli, the most common cause of UTI, which will help the physician in choosing the best antibiotic. Method. Total of 1,099 clean-catch mid stream urine (CCMSU) was processed by standard microbiological technique; 182 were from the diabetic group and 917 nondiabetic. Following identification, all isolates were subjected to antibiotic susceptibility testing using modified Kirby-Bauer disc diffusion method. In-vitro biofilm forming capacity of the isolates were detected by Microtitre plate method. The data were analyzed using SPSS software 16. Result. Urinary tract infection was found to be significantly higher in diabetic patients (42.9%) compared to nondiabetic patients (17.4%) with Escherichia coli as the most common uropathogen in both diabetic and nondiabetic groups. Similarly, UTI was more common in elderly population (29.5%). Imipenem, nitrofurantoin and amikacin were found to be the most effective drug for uropathogenic E. coli in both diabetic and nondiabetic patients, whereas amoxicillin, ciprofloxacin, and cotrimoxazole were least effective. Of the total bacterial isolates, 43.3% showed positive results for in-vitro biofilm production by the Microtitre plate method. A significantly higher resistance rate was observed among biofilm producing E. coli for quinolones, cotrimoxazole, and third generation cephalosporin ceftriaxone. Most of the biofilm producers (79.5%) were found to be MDR (p-value 0.015). Conclusion. Elderly populations with diabetes are at a higher risk of UTI. Higher biofilm production and resistance to in-use antimicrobial agents in this study render its inefficacy for empirical treatment and point out the importance of biofilm screening to ensure the effective management of infection.

scholarly journals Molecular typing methods to investigate transmission of Escherichia coli O157[ratio ]H7 from cattle to humans

1999 ◽  
Vol 123 (1) ◽  
pp. 17-24 ◽  
Author(s):  
M. LOUIE ◽  
S. READ ◽  
L. LOUIE ◽  
K. ZIEBELL ◽  
K. RAHN ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Profile Analysis ◽  
Diversity Indices ◽  
Plasmid Profile ◽  
Phage Typing ◽  
Direct Transmission ◽  
E Coli ◽  
Pfge Typing ◽  
Plasmid Analysis ◽  
Typing Methods

The utility of phage typing, pulsed-field gel electrophoresis (PFGE), and plasmid profile analysis was compared, to differentiate between Canadian Escherichia coli O157[ratio ]H7 strains of human (n = 27) and cattle (n = 24) origin. The diversity indices for phage typing, plasmid analysis and PFGE were 0·85, 0·69 and 0·93, respectively. PFGE and phage typing were also applied to study the role of direct transmission of E. coli O157[ratio ]H7 from cattle to humans on isolates collected from two separate farm outbreaks. PFGE showed that more than one E. coli O157[ratio ]H7 strain with varying PFGE DNA subtype profiles, may be responsible for an outbreak, and that more than one E. coli O157[ratio ]H7 subtype may be circulating on a particular farm at any one time. To our knowledge, this is one of the first reports where PFGE typing was used to verify the direct transmission of E. coli O157[ratio ]H7 from cattle to humans.

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