scholarly journals Bactericidal activity of a sanitary and hygienic product based on Bacillus subtilis bacteria strains

2020 ◽  
Vol 99 (3) ◽  
pp. 265-269
Author(s):  
Надежда Петровна Тарабукина ◽  
М.П. P. Неустроев ◽  
А.М. M. Степанова ◽  
С.И. I. Парникова ◽  
С.В. V. Дулова ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Bactericidal Activity ◽  
Bacterial Contamination ◽  
Animal Husbandry ◽  
Subclinical Mastitis ◽  
The Body ◽  
Bacterial Strains ◽  
Anaerobic Microorganisms ◽  
Dairy Cattle Breeding ◽  
Permafrost Soils

Introduction. A possible alternative to traditional methods of the treatment with chemical disinfectants are effective and safe preparations based on strains of bacteria Bacillus subtilis. The development of effective hygiene products based on Bacillus subtilis bacteria strains for air sanitation, household items, and rooms that do not adversely affect the body in the presence of humans, animals, and birds is an urgent task of sanitation and hygiene. Material and methods. Suspensions of an equal combination of the bacterial strains Bacillus subtilis TNP-3 and Bacillus subtilis TNP-5 were added to a solution of bicarbonate and sodium chloride. The strains were isolated from the permafrost soils of Yakutia, certified and deposited in the All-Russian collection of microorganisms used in animal husbandry and veterinary medicine (VGNKI, Moscow). The bactericidal activity of the «Probiodes 3 + 5» solutions was studied by the method of “coarse calico tests”. As test cultures there was used 2 billion suspension of diurnal cultures of Str. equi H-34 and Sal. abortus equi BN-12. The effectiveness of sanitization was taken into account according to the level of bacterial contamination - the number of mesophilic aerobic and facultative anaerobic microorganisms (KMAFAnM) on the skin of the nipples of the udder. Results. Reliable disinfection of coarse calico tests contaminated with Str. equi H-34, was established with the use of «Probiodez 3 + 5» (5x10 8 CFU / ml) for 6 hours exposure. By increasing the dose of B. subtilis in the solution «Probiodes 3 + 5» to (1.0 × 109 CFU / ml), there was reliable disinfection of coarse calico tests contaminated with Sal. abortus equi BN-12, exposure 6 hours. The drug «Probiodes 3 + 5» helps to reduce the total bacterial contamination and subclinical mastitis, effective for sanitary-hygienic treatment in dairy cattle breeding. Conclusion. The results of the studies allow making a conclusion that the bacterial strains B. subtilis TNP-3 and B. subtilis TNP-5 are promising for the development of effective, safe hygienic and sanitizing agents for widespread use.)

scholarly journals BACTERICIDAL ACTIVITY OF A SANITARY AND HYGIENIC PRODUCT BASED ON BACILLUS SUBTILIS BACTERIA STRAINS

2020 ◽  
Vol 99 (3) ◽  
pp. 265-269
Author(s):  
Nadezda P. Tarabukina ◽  
M. P. Neustroev ◽  
A. M. Stepanova ◽  
S. I. Parnikova ◽  
S. V. Dulova ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Bactericidal Activity ◽  
Bacterial Contamination ◽  
Animal Husbandry ◽  
Subclinical Mastitis ◽  
The Body ◽  
Bacterial Strains ◽  
Anaerobic Microorganisms ◽  
Dairy Cattle Breeding ◽  
Permafrost Soils

Introduction. A possible alternative to traditional methods of the treatment with chemical disinfectants are effective and safe preparations based on strains of bacteria Bacillus subtilis. The development of effective hygiene products based on Bacillus subtilis bacteria strains for air sanitation, household items, and rooms that do not adversely affect the body in the presence of humans, animals, and birds is an urgent task of sanitation and hygiene. Material and methods. Suspensions of an equal combination of the bacterial strains Bacillus subtilis TNP-3 and Bacillus subtilis TNP-5 were added to a solution of bicarbonate and sodium chloride. The strains were isolated from the permafrost soils of Yakutia, certified and deposited in the All-Russian collection of microorganisms used in animal husbandry and veterinary medicine (VGNKI, Moscow). The bactericidal activity of the «Probiodes 3 + 5» solutions was studied by the method of “coarse calico tests”. As test cultures there was used 2 billion suspension of diurnal cultures of Str. equi H-34 and Sal. abortus equi BN-12. The effectiveness of sanitization was taken into account according to the level of bacterial contamination - the number of mesophilic aerobic and facultative anaerobic microorganisms (KMAFAnM) on the skin of the nipples of the udder. Results. Reliable disinfection of coarse calico tests contaminated with Str. equi H-34, was established with the use of «Probiodez 3 + 5» (5x10 8 CFU / ml) for 6 hours exposure. By increasing the dose of B. subtilis in the solution «Probiodes 3 + 5» to (1.0 × 109 CFU / ml), there was reliable disinfection of coarse calico tests contaminated with Sal. abortus equi BN-12, exposure 6 hours. The drug «Probiodes 3 + 5» helps to reduce the total bacterial contamination and subclinical mastitis, effective for sanitary-hygienic treatment in dairy cattle breeding. Conclusion. The results of the studies allow making a conclusion that the bacterial strains B. subtilis TNP-3 and B. subtilis TNP-5 are promising for the development of effective, safe hygienic and sanitizing agents for widespread use.)

THE EFFECT OF PROBIOTIC FROM BACILLUS SUBTILIS STRAINS ON THE MINERAL AND VITAMIN COMPOSITION OF POULTRY PRODUCTSTHE EFFECT OF PROBIOTIC FROM BACILLUS SUBTILIS STRAINS ON THE MINERAL AND VITAMIN COMPOSITION OF POULTRY PRODUCTSTHE EFFECT OF PROBIOTIC FROM BACILLUS SUBTILIS STRAINS ON THE MINERAL AND VITAMIN COMPOSITION OF POULTRY PRODUCTSTHE EFFECT OF PROBIOTIC FROM BACILLUS SUBTILIS STRAINS ON THE MINERAL AND VITAMIN COMPOSITION OF POULTRY PRODUCTSTHE EFFECT OF PROBIOTIC FROM BACILLUS SUBTILIS STRAINS ON THE MINERAL AND VITAMIN COMPOSITION OF POULTRY PRODUCTSTHE EFFECT OF PROBIOTIC FROM BACILLUS SUBTILIS STRAINS ON THE MINERAL AND VITAMIN COMPOSITION OF POULTRY PRODUCTSTHE EFFECT OF PROBIOTIC FROM BACILLUS SUBTILIS STRAINS ON THE MINERAL AND VITAMIN COMPOSITION OF POULTRY PRODUCTSTHE EFFECT OF PROBIOTIC FROM BACILLUS SUBTILIS STRAINS ON THE MINERAL AND VITAMIN COMPOSITION OF POULTRY PRODUCTS

2020 ◽  
Vol 15 (8) ◽  
pp. 1128-1137
Author(s):  
A.M. Stepanova ◽  
Keyword(s):  
Bacillus Subtilis ◽  
Laying Hens ◽  
Poultry Meat ◽  
Control Group ◽  
Bacterial Strains ◽  
Poultry Products ◽  
Permafrost Soils ◽  
Calcium Magnesium ◽  
Hatching Eggs ◽  
Poultry Farming

The production of high-quality and environmentally friendly products is one of the urgent problems of industrial poultry farming. We have developed a probiotic preparation "Nord-Bact" based on bacterial strains Bacillus subtilis TNP-3 and Bacillus subtilis TNP-5 isolated from the permafrost soils of Yakutia for use in industrial poultry farming, starting with the processing of hatching eggs, including its use on young animals, chickens hens for the preservation, prevention and treatment of dysbacteriosis caused by pathogenic and opportunistic microflora. The aim of the work is to study the effect of the probiotic “Nord-Bact” on the mineral and vitamin composition of poultry products. Production tests were carried out at the Yakutsk Poultry Factory on 30 thousand laying hens of 45-46 weeks of age. Experienced groups of chickens took “Nord-Bact” probiotic at 5x107 CFU/bird daily, only with a difference in the timing of application: I - every 10 days for 2 months in a row, II - for 2 months. The control group did not take the probiotic. Also, young birds from 1 day old to 30 weeks of age in the experimental group received probiotic with water at the rate of 5x107 CFU/bird daily for 10 days every month in a row until the end of the experiment. All experiments were carried out on birds of the egg farming cross Rodonit-3. For biochemical studies, at the end of the experiments, samples of meat from pullets of 30 weeks of age and egg products from laying hens were taken. According to the results of the studies, it was found that after the application of the “Nord-Bact” probiotic in poultry meat, the content of the main macro and microelements (calcium, magnesium, phosphorus, iron, iodine, manganese, cobalt, copper, selenium, fluorine and zinc) and vitamins B12, A, E reliably rises compared with control. The use of the drug for laying hens markedly increases the amount of macro- and microelements, vitamins in the contents (white, yolk) and eggshells, compared with the control group. The use of the “Nord-Bact” probiotic in laying hens at a dose of 5x107 CFU/ bird for 10 days 2 months in a row increases the content of calcium, magnesium in the shell by 31% and phosphorus by 23%, and a significant increase of iron and vitamin A - by 12%, sodium - by 11%, magnesium - by 10%, potassium - by 6% and calcium - by 1.5% in the yolk compared to the control was also noted.

scholarly journals IMPROVING THE REPRODUCTIVE FUNCTION OF CATTLE BY PREVENTING COPPER DEFICIENCY IN THE BODY

2020 ◽  
pp. 170-177
Author(s):  
N.I. Erokhina ◽  
L.A. Zernaeva
Keyword(s):  
Dairy Cattle ◽  
Russian Federation ◽  
Biological Effect ◽  
Copper Deficiency ◽  
Reproductive Function ◽  
Animal Husbandry ◽  
The Body ◽  
Cattle Breeding ◽  
Dairy Cattle Breeding ◽  
The Russian Federation

In the dairy cattle breeding of the Russian Federation, there are unresolved problems associated with the reproduction of the herd, which significantly affect the economic component of this subsector of animal husbandry. One of the factors causing impaired reproductive function in animals is the lack of copper in the body. The presented materials indicate the importance of the prevention of copper deficiency in animals and further research in the field of the biological effect of this microelement on reproductive function.

scholarly journals Study of the resistance of cows with mastitis depending on the age of lactation

2022 ◽  
Vol 354 (11-12) ◽  
pp. 27-31
Author(s):  
M. V. Dovydenkova
Keyword(s):  
Blood Serum ◽  
Immune Status ◽  
Animal Husbandry ◽  
Subclinical Mastitis ◽  
Clinical Mastitis ◽  
The Body ◽  
Nonspecific Resistance ◽  
Animal Blood ◽  
Black And White ◽  
Holstein Breed

Relevance. The breeding stock and young cattle in the conditions of industrial technology of animal husbandry experience an increased antigenic load, which leads to disturbances in the immune status and the development of inflammatory processes manifested by endometritis and mastitis, diarrheal and respiratory syndromes. It was relevant to conduct a comprehensive assessment of the immunological reactivity of the animal’s body according to the state of resistance in cattle with clinical and subclinical mastitis, depending on the age of lactation. As a result of complex studies, the specific and nonspecific reactivity of the body of cattle in different periods of lactation was studied.Methods. The object of the study were cows of a black-and-white Holstein breed (n = 450). Groups of animals were formed according to the age of lactation:1st lactation, 2–3rd lactation, 3–4th lactation and for health reasons: clinically healthy animals, with subclinical mastitis and clinical mastitis. Milk samples were taken from cows to count the number of somatic cells, and blood to determine the quantitative content of IgG immunoglobulin and indicators of nonspecific resistance.Results. It was found that with mastitis in cows of different lactation ages, almost identical changes in the immune status were observed. Quantitative determination of IgG levels in animal blood serum is the basis for assessing the immunity of cattle. The immune status in clinically healthy animals was characterized by a higher level of the total amount of IgG immunoglobulin in the blood serum (amounted to 22.25 ± 0.70 mg/ml), whereas in cows with a subclinical form of mastitis, the level of IgG immunoglobulin decreased by 12.3%, with a clinical form of mastitis — by 17.2%. The results also showed that in cows of different lactations with subclinical and clinical mastitis, the bactericidal activity (by 10.8%) and the lysozyme activity of the blood increased significantly (by 8.22%). In cows with clinical mastitis — by 8.6% and 9.7% respectively, compared with the indicators in groups of healthy animals. Thus, mastitis, occurring in acute and subacute form, leads to a decrease in the resistance of the cows’ organism.

scholarly journals Intracellular serine proteinase of Bacillus subtilis strain Marburg 168. Comparison with the homologous enzyme from Bacillus subtilis strain A-50

1979 ◽  
Vol 179 (2) ◽  
pp. 333-339 ◽  
Author(s):  
A Y Strongin ◽  
D I Gorodetsky ◽  
I A Kuznetsova ◽  
V V Yanonis ◽  
Z T Abramov ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Serine Proteinase ◽  
Subtilis Strain ◽  
Bacterial Strains ◽  
Terminal Sequence ◽  
Gramicidin S ◽  
Intracellular Enzymes ◽  
Sepharose 4B ◽  
Homologous Enzyme ◽  
Strict Selection

Intracellular serine proteinase was isolated from sporulating cells of Bacillus subtilis Marburg 168 by gramicidin S-Sepharose 4B affinity chromatography. The enzymological characteristics, the amino acid composition and the 19 residues of the N-terminal sequence of the enzyme are reported. The isolated proteinase was closely related to, but not completely identical with, the intracellular serine proteinase of B. subtilis A-50. The divergence between these two intracellular enzymes was less than that between the corresponding extracellular serine proteinases (subtilisins) of types Carlsberg and BPN′!, produced by these bacterial strains. This may be connected with the more strict selection constraints imposed in intracellular enzymes during evolution.

Bactericidal substance produced by Haemophilus influenzae b

1975 ◽  
Vol 21 (10) ◽  
pp. 1587-1594 ◽  
Author(s):  
R. A. Venezia ◽  
R. G. Robertson
Keyword(s):  
Haemophilus Influenzae ◽  
Bactericidal Activity ◽  
High Speed ◽  
Indicator Strain ◽  
Bacterial Strains ◽  
Small Molecular Weight ◽  
Plaque Test ◽  
Haemophilus Species ◽  
Phage Propagation ◽  
Thermostable Protein

During bacteriophage studies on Haemophilus influenzae, it was observed that encapsulated type b and unencapsulated Rb strains released a bactericidal substance active against types a, c, d, e, and f H. influenzae, non-typable H. influenzae strains, other Haemophilus species, and certain members of the Enterobacteriaceae. The bactericidal activity was assayed by a plaque test utilizing an Rd strain as an indicator lawn and was also demonstrated in mixed broth cultures of a producer strain and an indicator strain. Immediate lysis of sensitive bacteria by the factor was not evident. The factor is sensitive to trypsin but resistant to deoxyribonuclease, treatment with 2-mercaptoethanol, lipase, α-amylase, and heating in a 100 °C water bath for 20 min. The activity is not dependent upon increased Ca2+ or Mg2+ concentration as is necessary for HP1C1 and S2 phage propagation. The bactericidal factor is not pelleted by high-speed centrifugation at 150 000 × g for 6 h. Treatment with ultraviolet light or mitomycin C does not result in observable phage, phage-like particles, or increased bactericidal activity. The bactericidal factor is not a typical small molecular weight "colicin-like" bacteriocin in that it is not inducible, has a wider range of activity, and does not kill by "single-hit" kinetics. On preliminary characterization, it is a thermostable protein toxic to certain bacterial strains.

A Review on Meropenem

2021 ◽  
pp. 5034-5038
Author(s):  
Mohsina Abed ◽  
Sara Yousuf
Keyword(s):  
Intravenous Administration ◽  
Wide Spectrum ◽  
Gastrointestinal Haemorrhage ◽  
The Body ◽  
Antibacterial Drug ◽  
Bacterial Strains ◽  
Enhanced Absorption ◽  
Bacterial Cell Death ◽  
Wide Range ◽  
Synthetic Derivative

Meropenem is a new Carbapenem antibacterial agent with wide spectrum of activity for intravenous administration. It is synthetic derivative of Thienamycin. Three analogues of Meropenem are evaluated and active against 18 bacterial strains. Meropenem causes rapid bacterial cell death by covalently binding to penicillin binding proteins (PBS). Structural modification at C-2 position, produced double promoiety prodrug of Meropenem and increases bioavailability of oral administration. Other forms of drug such as liposome and nanoparticles are also available with enhanced absorption. 14C labelled Meropenem prepared from 14C Dimethylamine hydrochloride is used for the analysis of M. tuberculosis transpeptidase. ICI213,689 is the only metabolite of Meropenem and it is inactive. Meropenem penetrates well into the body fluids and tissues including cerebrospinal fluid. Its bioavailability is 100% on intravenous administration. Hence it is used in the treatment of meningitis, febrile neutropenia, anthrax and various other skin and skin structure infections. Dosage reduction is required in patient with reduced renal function but not in hepatic impairment. Seizures, gastrointestinal haemorrhage are observed in patients. Vabmoere is the combination of Meropenem and Vaborbactam which is active against the Carbapenem resistant Enterobacteriacea. Meropenem is an effective broad-spectrum antibacterial drug for the treatment of wide range of infection including polymicrobial infection in both children and adult.

scholarly journals Characterization of the Antibacterial Activity of an SiO2 Nanoparticular Coating to Prevent Bacterial Contamination in Blood Products

Antibiotics ◽  
2022 ◽  
Vol 11 (1) ◽  
pp. 107
Author(s):  
Sahra Fonseca ◽  
Marie-Pierre Cayer ◽  
K. M. Tanvir Ahmmed ◽  
Nima Khadem-Mohtaram ◽  
Steve J. Charette ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Bacterial Contamination ◽  
Polymeric Materials ◽  
L929 Cell ◽  
Bacterial Strains ◽  
Transmitted Infection ◽  
Log Reduction ◽  
Plasticized Pvc ◽  
Transfusion Transmitted Infection ◽  
Field Electron Microscopy

Technological innovations and quality control processes within blood supply organizations have significantly improved blood safety for both donors and recipients. Nevertheless, the risk of transfusion-transmitted infection remains non-negligible. Applying a nanoparticular, antibacterial coating at the surface of medical devices is a promising strategy to prevent the spread of infections. In this study, we characterized the antibacterial activity of an SiO2 nanoparticular coating (i.e., the “Medical Antibacterial and Antiadhesive Coating” [MAAC]) applied on relevant polymeric materials (PM) used in the biomedical field. Electron microscopy revealed a smoother surface for the MAAC-treated PM compared to the reference, suggesting antiadhesive properties. The antibacterial activity was tested against selected Gram-positive and Gram-negative bacteria in accordance with ISO 22196. Bacterial growth was significantly reduced for the MAAC-treated PVC, plasticized PVC, polyurethane and silicone (90–99.999%) in which antibacterial activity of ≥1 log reduction was reached for all bacterial strains tested. Cytotoxicity was evaluated following ISO 10993-5 guidelines and L929 cell viability was calculated at ≥90% in the presence of MAAC. This study demonstrates that the MAAC could prevent bacterial contamination as demonstrated by the ISO 22196 tests, while further work needs to be done to improve the coating processability and effectiveness of more complex matrices.

scholarly journals Imaging Sensitive and Drug-Resistant Bacterial Infection with [11C]-TMP: In Vitro and First-in-Human Evaluation

2021 ◽  
Author(s):  
Iris K Lee ◽  
Daniel A Jacome ◽  
Joshua K Cho ◽  
Vincent Tu ◽  
Anthony Young ◽  
...  
Keyword(s):  
Bacterial Infection ◽  
Mammalian Cells ◽  
Bacterial Infections ◽  
The Body ◽  
Response Monitoring ◽  
Drug Resistant ◽  
Bacterial Strains ◽  
Critical Question ◽  
In Vitro Uptake

Recently, several molecular imaging strategies have developed to image bacterial infections in humans. Nuclear approaches, specifically positron emission tomography (PET), affords sensitive detection and the ability to non-invasively locate infections deep within the body. Two key radiotracer classes have arisen: metabolic approaches targeting bacterial specific biochemical transformations, and antibiotic-based approaches that have inherent selectivity for bacteria over mammalian cells. A critical question for clinical application of antibiotic radiotracers is whether resistance to the template antibiotic abrogates specific uptake, thus diminishing the predictive value of the diagnostic test. We recently developed small-molecule PET radiotracers based on the antibiotic trimethoprim (TMP), including [11C]-TMP, and have shown their selectivity for imaging bacteria in preclinical models. Here, we measure the in vitro uptake of [11C]-TMP in pathogenic susceptible and drug-resistant bacterial strains. Both resistant and susceptible bacteria showed similar in vitro uptake, which led us to perform whole genome sequencing of these isolates to identify the mechanisms of TMP resistance that permit retained radiotracer binding. By interrogating these isolate genomes and a broad panel of previously sequenced strains, we reveal mechanisms where uptake or binding of TMP radiotracers can potentially be maintained despite the annotation of genes conferring antimicrobial resistance. Finally, we present several examples of patients with both TMP-sensitive and drug-resistant infections in our first-in-human experience with [11C]-TMP. This work underscores the ability of an antibiotic radiotracer to image bacterial infection in patients, which may allow insights into human bacterial pathogenesis, infection diagnosis, and antimicrobial response monitoring.

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