scholarly journals Assessment of changes in blood plasma biochemical indices at hypercholesterol diet with a high fat load

2021 ◽  
Vol 100 (6) ◽  
pp. 617-622
Author(s):  
Natalya F. Kushnerova ◽  
Yury A. Rakhmanin ◽  
Tatiana V. Momot ◽  
Rufina I. Mikhailova ◽  
Irina N. Ryzhova ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Biochemical Parameters ◽  
Fatty Acid Esters ◽  
Male Rats ◽  
High Density Lipoproteins ◽  
Cholesterol Diet ◽  
High Fat ◽  
Lipid Complex ◽  
The Impact ◽  
In Blood Plasma

Introduction. It was studied the lipid composition of the blood plasma of rats under the impact of a hyper cholesterol diet with a high fat load. It was carried out the prevention of disturbances in blood plasma biochemical parameters with a lipid complex from the tunic of the marine hydrobiont Halocynthia aurantium. Materials and methods. The experiment was carried outwith outbred male rats weighing 200 ± 3 g.The experimental model of a hyper cholesterol diet with a high fat load with the development of dyslipidemia was set up by feeding the animals with ahigh fat diet consisting of 2% cholesterol and 20% beef fat from the total diet. The animals were divided into the following groups of 10 rats each: group 1 - control (standard diet), group 2 - dyslipidemia (hypercholesterol diet with high fat load), group 3 - dyslipidemia + lipid complex from ascidia. Results. It was shown that the influence of the diet was accompanied by an increase in the amount of total lipids in the blood plasma of rats, cholesterol, low-density lipoproteins (LDL), as well as a decrease in total phospholipids and high-density lipoproteins (HDL), which is considered as an indicator of the formation of dyslipidemia. The contents of phospholipid lysofractions increased due to the activation of phospholipases. The amount of fatty acid esters and cholesterol esters decreased, which indicates the inhibition of esterification processes. The imbalance in the phospholipid spectrum of blood plasma occurred: the amount of metabolically active fractions required for the functioning of membrane-bound enzymes decreased. The addition of a lipid complex from the tunic of ascidian purple into the diet was accompanied by a pronounced prophylactic effect, which manifested itself in the normalization of the studied biochemical parameters. The lipid complex containing a wide range of “sea” phospholipids and polynonsaturated fatty acids of the n-3 type is an important basis for application as prophylactic in the conditions of a hypercholesterol diet with a high-fat load. Conclusion. Application of the lipidic complexes containing the “sea” lipids allocated from a tunic of the ascidian purple can be useful and perspective at a dislipidemiya and a hypercholesterolemia that will allow to carry out effective prevention of violations of metabolic reactions at influence of hyper high-calorie food.

scholarly journals INFLUENCE OF ALIMENTARY INSUFFICIENCY OF CALCIUM, MAGNESIUM AND IODINE ON RAT VITAMIN STATUS

2021 ◽  
Vol 22 (1) ◽  
pp. 52-59
Author(s):  
O.A. Vrzhesinskaya ◽  
◽  
V.M. Kodentsova ◽  
N.A. Beketova ◽  
O.V. Kosheleva ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Vitamin B1 ◽  
Male Rats ◽  
Molar Ratio ◽  
Vitamin Status ◽  
Iron And Zinc ◽  
Calcium Magnesium ◽  
Increased Activity ◽  
Experimental Group ◽  
In Blood Plasma

The diet of the majority of the population of Russia is characterized by an insufficient content of a number of micronutrients, including macro- and micronutrients. The aim of the study is to characterize in a rat model experiment the effect of combined nutritional deficiency of calcium, magnesium and iodine on the availability of other mineral substances, vitamin metabolism and biochemical parameters blood plasma. The lack of minerals in growing Wistar male rats with an initial body weight (51.4 ± 0.5) g was created for 23 days by halving of a calcium, magnesium and iodine in the salt mixture. Alimentary deficiency of calcium, magnesium and iodine in growing rats significantly and multidirectionally affected the metabolism of other minerals and vitamins, as well as a number of diagnostically significant indicators of blood and urine. In rats of the experimental group, the liver concentration of α-tocopherol, vitamin B1 and retinol palmitate decreased, the concentration of iron and zinc increased, the copper content in the whole brain decreased significantly. The molar ratio of γ- and α-tocopherols in blood plasma was increased, the concentration of the circulating form of vitamin D decreased. The ratio of AlAT / AsAT (de Ritis coefficient) markedly decreased due to increased activity of AlAT. Phosphate reabsorption decreased slightly. Increased urinary excretion of riboflavin was accompanied by a decrease in its concentration in blood plasma. The conclusion that insufficient intake of calcium, magnesium and iodine may disturb vitamin-mineral status of organism has been made.

scholarly journals Role of fibronectin under conditions of doxorubicin action

2015 ◽  
Vol 6 (1) ◽  
pp. 17-22
Author(s):  
A. I. Shevtsova ◽  
G. A. Ushakova
Keyword(s):  
Blood Plasma ◽  
Total Protein ◽  
Protein Concentration ◽  
Male Rats ◽  
Antitumor Action ◽  
Total Protein Concentration ◽  
Monospecific Antibodies ◽  
Anthracycline Chemotherapy ◽  
In Blood Plasma

There is no standard as to treatment of anthracycline chemotherapy complications. The reduction of cytotoxic drugs toxicity without weakening of their antitumor action remains relevant. The extracellular matrix which key component is fibronectin is present in all tissues and it continuously undergoes controlled remodeling. So, the purpose of our work was to study the level of fibronectin in the experimental model of doxorubicin-induced cardiomyopathy and effects of this cytostatic and its co-administration with antioxidants of different nature.The level of fibronectin was measured by ELISA using monospecific antibodies against fibronectin (Sigma, USA), secondary anti-IgG labeled with horseradish peroxidase (Sigma, USA) and fibronectin standard (Sigma, USA). The study was conducted on Wistar male rats with weight of 210 ± 50 g which were divided into 4 groups by 8 animals in each group: 1 – control, rats receiving saline i/p; 2 – doxorubicin 1 mg/kg i/p once a week during 4 weeks; 3 – doxorubicin by the same scheme plus 1% 2-oxoglutarate in drinking water during 4 weeks;4 – doxorubicin by the same scheme and korvitin injection 30 min before doxorubicin application once a week during 4 weeks. Obtained data indicate the effect of doxorubicin to decrease in index mass heart in 38% of animals compared to control animals; decrease in total protein concentration by 8% (Р < 0,05) and increase of the level of fibronectin by 67% (P < 0,001) in blood plasma of rats and decrease in the level of fibronectin in the heart extract by 19% (Р < 0,05) under development of doxorubicin-induced cardiotoxicity. Increased fibronectin concentration in blood plasma had strong correlation with decreased total protein concentration in blood (r=0,80) and heart extract (r=0,59) in rats with doxorubicin-induced cardiomiophaty indicating the sensitive reaction of fibronectin to development of metabolic disorders under doxorubicin influence. 

scholarly journals High-fat diet-induced hypertension is associated with a proinflammatory T cell profile in male and female Dahl salt-sensitive rats

2018 ◽  
Vol 315 (6) ◽  
pp. H1713-H1723 ◽  
Author(s):  
Lia E. Taylor ◽  
Ellen E. Gillis ◽  
Jacqueline B. Musall ◽  
Babak Baban ◽  
Jennifer C. Sullivan
Keyword(s):  
T Cells ◽  
T Cell ◽  
Regulatory T Cells ◽  
High Fat Diet ◽  
Female Rats ◽  
Male Rats ◽  
High Fat ◽  
Male And Female ◽  
The Impact ◽  
Cell Profile

Evidence supports a sex difference in the impact of a high-fat diet (HFD) on cardiovascular outcomes, with male experimental animals exhibiting greater increases in blood pressure (BP) than female experimental animals. The immune system has been implicated in HFD-induced increases in BP, and there is a sex difference in T-cell activation in hypertension. The goal of this study was to determine the impact of HFD on BP and aortic and renal T cell profiles in male and female Dahl salt-sensitive (DSS) rats. We hypothesized that male DSS rats would have greater increases in BP and T cell infiltration in response to a HFD compared with female DSS rats. BP was measured by tail-cuff plethysmography, and aortic and renal T cells were assessed by flow cytometric analysis in male and female DSS rats on a normal-fat diet (NFD) or HFD from 12 to 16 wk of age. Four weeks of HFD increased BP in male and female DSS rats to a similar degree. Increases in BP were accompanied by increased percentages of CD4+ T cells and T helper (Th)17 cells in both sexes, although male rats had more proinflammatory T cells. Percentages of renal CD3+ and CD4+ T cells as well as Th17 cells were increased in both sexes by the HFD, although the increase in CD3+ T cells was greater in male rats. HFD also decreased the percentage of aortic and renal regulatory T cells in both sexes, although female rats maintained more regulatory T cells than male rats regardless of diet. In conclusion, both male and female DSS rats exhibit BP sensitivity to a HFD; however, the mechanisms mediating HFD-induced increases in BP may be distinct as male rats exhibit greater increases in the percentage of proinflammatory T cells than female rats. NEW & NOTEWORTHY Our study demonstrates that male and female Dahl salt-sensitive rats exhibit similar increases in blood pressure to a high-fat diet and an increase in aortic and renal T cells. These results are in contrast to studies showing that female rats remain normotensive and/or upregulate regulatory T cells in response to hypertensive stimuli compared with male rats. Our data suggest that a 4-wk high-fat diet has sex-specific effects on the T cell profile in Dahl salt-sensitive rats.

scholarly journals FREE AMINO ACIDS OF BLOOD PLASMA AS AN INTEGRAL PARAMETER OF METABOLIC DISORDERS IN A LONG-TERM INTAKE OF SMALL DOSES OF LEAD ACETATE INTO THE ORGANISM

2017 ◽  
pp. 67-71
Author(s):  
V. M. Sheybak ◽  
A. Y. Pavliukovets ◽  
V. Yu. Smirnov
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Blood Plasma ◽  
Free Amino Acids ◽  
Free Amino ◽  
Lead Acetate ◽  
Male Rats ◽  
Integral Parameter ◽  
In Blood Plasma

Objective: to analyze the pool of free amino acids and their nitrogen-containing metabolites of blood plasma of animals being administered lead acetate for 21 or 28 days. Material and methods. The experiments were carried out on white male rats with the initial weight of 140-160 g. During the experiment the animals received a total amount of 0.67 g of lead acetate (21 days), the second group - 1.12 g (28 days). The determination of free amino acids in blood plasma was performed by the method of reversed-phase HPLC. Results. As a result of the research it has been found out that the most significant contribution to the formation of the amino acid imbalance in blood plasma as a result of the long-term intake of lead acetate according to the value of the Fisher test is caused by changes in the concentrations of α-aminobutyric acid, isoleucine, aspartate, arginine, glycine, serine, histidine, and methionine. Conclusion. The long-term intake of lead acetate with drinking water leads to a statistically significant increase of general content of free amino acids and their nitrogen-containing metabolites, as well as to a decrease of the concentration the essential amino acid threonine in blood plasma.

Impact of Long-term, High-fat, and High-cholesterol Diet on Murine Vertebrae Bones

2020 ◽  
Author(s):  
Frank Alexander Schildberg ◽  
Koroush Kabir ◽  
Jessica Bojko ◽  
Mona Khoury ◽  
Werner Masson ◽  
...  
Keyword(s):  
Bone Density ◽  
Genetic Factors ◽  
Control Group ◽  
High Cholesterol Diet ◽  
Micro Ct ◽  
Cholesterol Diet ◽  
High Cholesterol ◽  
High Fat ◽  
The Impact

AbstractAs the percentage of overweight individuals in the population rises, diseases associated with excess weight resulting from poor nutrition are becoming more and more widespread. So far, the influence of weight or nutrition on bone health has shown conflicting results. In the literature, the existing studies disagree about the effect of diet on bones. Therefore, this study investigated the impact of a long-term, high-fat, and high-cholesterol diet on the spine in a mouse model. Wild-type mice were randomly separated into two groups; one group received a high-fat and high-cholesterol diet, and a control group was fed with a regular diet since birth for a duration of 8 months. The first to fifth thoracic vertebrae were extracted and investigated using histology and micro-CT. Samples were analyzed regarding different parameters: percentage of bone structure compared to the whole vertebra and the amount and thickness of the trabeculae. Both methods of the analysis showed similar results. Diet did not have a significant impact on the bone density of the vertebrae. The micro-CT examination showed that the average bone percentage of the examined vertebra was 6% (p = 0.2330) higher in the control group compared to the diet group. The same tendency was demonstrated in histology even though with a smaller difference of only 5%. The results of both methods were comparable and showed trends for the influence of different diets but not significant impacts. In summary, this study showed that a high-fat and high-cholesterol diet has a slightly negative impact on bone density. In order to further analyze the effects of different diets on bone composition, structure, and density, additional long-term studies should be carried out, and more parameters such as movement and genetic factors should be analyzed. Furthermore, other parameters such as exercise and genetic factors that could have a secondary influence on obesity should be investigated.

scholarly journals The effect of cholesterol-enriched diet on the activity of some lysosomal enzymes in the liver and blood plasma of rabbits

2002 ◽  
Vol 45 (2) ◽  
pp. 199-203
Author(s):  
A. M. Konecka ◽  
T. Jezierski ◽  
A. Śliwa-Jóźwik ◽  
A. Jóźwik ◽  
A. Kołątaj
Keyword(s):  
New Zealand ◽  
Blood Plasma ◽  
Lysosomal Enzymes ◽  
Control Level ◽  
Control Group ◽  
High Cholesterol Diet ◽  
Cholesterol Diet ◽  
High Cholesterol ◽  
In Blood Plasma

Abstract. White New Zealand male rabbits (n = 12) were fed by high-cholesterol diet for 7 weeks. The activity of some lysosomal enzymes in blood plasma and in the liver was determined. The cholesterol-enriched diet resulted a significant of AP, LL, BGAL and BGLU activity up increase in the blood plasma to 131 %, 186 %, 308 % and 184 % as compared with those in control group of animals (n = 10). In the liver a significant increase of EL activity to 189 %, NAG to 172% and BGAL to 196 % was observed. The activity of LAP and LL decreased significantly to 76 % and 60 % of the control level, respectively.

scholarly journals Blood lipid profile of female and male rats in case of toxic cardiomyopathy, due to the introduction of sodium nitrite

2017 ◽  
Author(s):  
I. Ya. Krynytska ◽  
M. I. Marushchak ◽  
I. O. Stahurska ◽  
I. R. Bekus ◽  
M. V. Kyryliv ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Lipid Profile ◽  
Sodium Nitrite ◽  
Plasma Lipid ◽  
Density Lipoprotein ◽  
Blood Lipid ◽  
Male Rats ◽  
Cholesterol Concentration ◽  
High Density Lipoproteins ◽  
Blood Lipid Profile

The indices of the blood plasma lipid profile of female and male rats in case of toxic cardiomyopathy, due to the introduction of sodium nitrite were sudied. It was determined the increasing of cholesterol concentration in the blood plasma and its redistribution between lipoproteins due to reduction in high density lipoproteins and an increase in low-density lipoprotein, which have proatherogenic properties in animals of both sexes. In gender comparison of changes in blood lipid profile in case of sodium nitrite intoxication their significant prevalence in male rats was found. 

scholarly journals Hematological parameters in rats poisoned with cadmium nitrate and in biocorrection with the infusion of Beta vulgaris seeds

2020 ◽  
Vol 100 (4) ◽  
pp. 94-99
Author(s):  
Aiman Konkabayeva ◽  
◽  
Aidana Erubay ◽  
Еvgenii Gaibel ◽  
◽  
...  
Keyword(s):  
Beta Vulgaris ◽  
Biochemical Parameters ◽  
Molecular Mechanisms ◽  
Hematological Parameters ◽  
Acute Poisoning ◽  
Male Rats ◽  
Cadmium Nitrate ◽  
The Difference ◽  
Student’S T ◽  
The Impact

Nowadays, using plant extracts and infusions as protectors against the impact of heavy metals, the molecular mechanisms, protective and adaptive reactions of plants are studied. The purpose of this article was to study the protective properties of beetroot seeds infusion in acute and sub-acute poisoning of rats with cadmium nitrate. For this experiment, we used 50 male rats. The animals were exposed to Cd nitrate, with an initial weight of 180 ± 30 g. The first groups of rats were injected with cadmium nitrate at a dose of 0.1 g/L intraperitoneally, the second groups received a 0.01 % solution of cadmium nitrate, 1 mL, 5 days a week, orally for 10 and 24 days. General blood tests and biochemical parameters in rats were investigated. By biochemical parameters, we determined liver enzymes alanine aminotransferase (ALT), aspartate aminotransferase (AST), bilirubin, glucose, urea, creatinine, and total protein. The mean ± SEM values were calculated for each group to determine the significance of the intergroup difference. Each parameter was analyzed separately using the one-way analysis of variance (ANOVA) test. To determine the difference between groups, Student’s «t»-test was used. Studies showed the presence of variations in the parameters of leukocytes, erythrocytes and platelets depending on the period of days of poisoning and biocorrection. There were small changes in the content of urea, creatinine, and protein, which were not reliably confirmed. As a result, our research allowed asserting that the use of Beta vulgaris infusion in acute and subacute experiments with poisoning of animals with cadmium nitrate had a positive hepatoprotective effect.

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