Effect of Various Immersion Time and Water Temperature on Seed Germination of Clitoria ternatea and Momordica charantia

The seeds of Clitoria ternatea and Momordica charantia were subjected to seven pre-sowing treatments, i.e., control (T0), peeled coat and soaked in 5 oC for 24 hours (T1), peeled coat and soaked in 37 oC for 24 hours (T2), peeled coat and soaked in 5 oC for 48 hours (T3), peeled coat and soaked in 37 oC for 48 hours (T4), peeled coat and soaked in 5 oC for 48 hours (T5), peeled coat and soaked in 37 oC for 72 hours (T6). The study revealed that peeling the coat and soaking seeds in water for various temperatures and periods improved seed germination. The highest germination and germination energy percentage of C. ternatea were observed in T2, namely 94.95% and 23.69%, respectively, while the lowest germination (0%) and germination energy (0%) was found in T6. The highest germination and germination energy percentage of M. charantia, namely 64.38%, and 16.10%, respectively, were found in T3, while the lowest germination (10.67%) and germination energy (2.17%) were observed in T0. The germination may vary for both seeds used in the study as C. ternatea and M. charantia are different in type. The pre-sowing treatments of seeds would prove its potential in the practical fields.

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METHODS OF RAISING SEED GERMINATION RATE AND GERMINATION ENERGY IN CROPS FROM THE FAMILY Brassicaceae Burnett: (GUIDELINES)

10.30901/978-5-907145-43-6 ◽

2019 ◽

Author(s):

D.L. Kornyukhin ◽

◽

A.M. Artemeva ◽

S.S. Abremskaya ◽

◽

...

Keyword(s):

Seed Germination ◽

Germination Rate ◽

Germination Energy ◽

The Family

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Scarification with sulphuric acid of Schizolobium amazonicum Huber ex Ducke seeds - FABACEAE

Scientia Agricola ◽

10.1590/s0103-90162007000300017 ◽

2007 ◽

Vol 64 (3) ◽

pp. 308-313 ◽

Cited By ~ 7

Author(s):

Eniel David Cruz ◽

José Edmar Urano de Carvalho ◽

Rafaela Josemara Barbosa Queiroz

Keyword(s):

Statistical Analysis ◽

Experimental Design ◽

Seed Germination ◽

Sulphuric Acid ◽

Seed Coat ◽

Immersion Time ◽

Germination Index ◽

Efficient Treatment ◽

Tukey Test

Seed coat impermeability to water occurs in many species, including Schizolobium amazonicum Huber ex Ducke. To promote germination in seeds with coat impermeability the use of sulphuric acid (H2SO4) is recommended. The objective of this study was to identify a better time for the scarification with sulphuric acid for S. amazonicum seeds. The effect of scarification with sulphuric acid for 20, 40 and 60 min on germination and speed germination was studied for seeds that were either sowed immediately after scarification or after a 24-hour period of immersion in water. Seeds were sown on a mix of sand and sawdust (1:1). The experimental design was completely randomized with four replications of 50 seeds. The statistical analysis of germination was carried out at six, nine, 12, 15, 18, 21 and 24 days after sowing, in a factorial scheme. For speed germination the means were compared by the Tukey test. There was an interaction between treatments to overcome dormancy and immersion time after scarification in most evaluations. Immersion in water accelerated the beginning of germination. All treatments to overcome dormancy promoted seed germination. However, scarification for 60 min, showed better germination, 92% when immediately sown and 86.5% when sown after 24 hours. Speed germination index was highest for scarified seeds for 60 min followed by immersion in water. Scarification for 60 min was the most efficient treatment to promote germination in S. amazonicum seeds.

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QUALIDADE SANITÁRIA DE SEMENTES DE Caesalpinia ferrea: INCIDÊNCIA DE FUNGOS, CONTROLE E EFEITOS NA QUALIDADE FISIOLÓGICA COM O USO DE EXTRATOS VEGETAIS

FLORESTA ◽

10.5380/rf.v45i1.34074 ◽

2014 ◽

Vol 45 (1) ◽

pp. 163 ◽

Cited By ~ 2

Author(s):

José George Ferreira Medeiros ◽

Aderson Costa Araujo Neto ◽

Edcarlos Camilo Silva ◽

Min Fu Nascimento Huang ◽

Luciana Cordeiro Nascimento

Keyword(s):

Seed Germination ◽

Aspergillus Flavus ◽

Plant Extracts ◽

Germination Rate ◽

Antimicrobial Properties ◽

Momordica Charantia ◽

Alternative Control ◽

Penicillium Sp ◽

Physiological Quality

As sementes são eficientes meios de disseminação e transmissão de patógenos e, frequentemente, introduzem novos focos de infecção em áreas isentas. A utilização de extratos de plantas com propriedades antimicrobianas são alternativas ecológicas e promissoras para substituir a proteção promovida pela aplicação de fungicidas. Assim, objetivou-se avaliar o efeito dos extratos de alamanda (Allamanda blanchetti L.) e melão-de-são-caetano (Momordica charantia L.) sobre a micoflora e a germinação de sementes de Caesalpinia ferrea. Para tanto, utilizaram-se lotes de sementes coletados em diferentes municípios paraibanos (Areia, Bananeiras, Conde e Remígio), os quais foram submetidos aos testes de sanidade e de germinação. Os tratamentos fitossanitários consistiram de Testemunha; Fungicida dicarboximida (240 g.100 kg-1) e extratos A. blanchetti e M. charantia nas concentrações de 10, 100, 500 e 1000 ppm. Constataram-se os fungos Aspergillus flavus, A. niger, Cladosporium sp., R. stolonifer, Penicillium sp. e Nigrospora sp. nas sementes de C. ferrea. Os extratos de A. blanchetti e M. charantia nas concentrações de 500 e 1000 ppm reduziram a incidência de fungos. As maiores concentrações (500 e 1000 ppm) de ambos os extratos promoveram o aumento no percentual e na velocidade de germinação (IVG), bem como no comprimento das plântulas de C. ferrea.Palavras-chave: Patologia de sementes; micoflora; controle alternativo; germinação. AbstractSanitary quality of Caesalpinia ferrea seeds: incidence of fungi, control and effects on physiological quality with the use of plant extracts. Seeds are efficient to dissemination and transmission of pathogens and often introduce new foci of infection in exempted areas. The use of plant extracts with antimicrobial properties are promising and environmentally friendly alternatives to replace the protection promoted by the application of fungicides. We aimed to evaluate the effect of extracts of Allamanda blanchetti L. and Momordica charantia L. on the mycoflora and seed germination of Caesalpinia ferrea. We used lots of seeds collected in different cities of Paraiba (Areia, Bananeiras, Conde e Remígio), which were tested for germination and sanity. The phytosanitary treatments consisted of control, dicarboximide fungicide (240 g.100 Kg-1) and extracts of A. blanchetti and M. charantia at concentrations of 10, 100, 500 and 1000 ppm. We identified in the seeds of C. ferrea fungi Aspergillus flavus, A. niger, Cladosporium sp., R. stolonifer, Penicillium sp. and Nigrospora sp. The extracts of A. blanchetti and M. charantia in concentrations of 500 and 1000 ppm reduced the incidence of fungi. The highest concentrations (500 and 1000 ppm) of both extracts increased percentage and germination rate (IVG) and length of seedlings of C. ferrea.Keywords: Pathology seed; mycoflora; alternative control; germination.

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BIOLOGY OF SEEDS OF REPRESENTATIVES OF GENUS HYDRANGEA L. IN THE SOUTH URAL BOTANICAL GARDEN (UFA)

Вестник Пермского университета. Серия «Биология»=Bulletin of Perm University. Biology ◽

10.17072/1994-9952-2020-1-13-18 ◽

2020 ◽

pp. 13-18

Author(s):

F. K. Murzabulatova ◽

◽

N. V. Polyakova ◽

Keyword(s):

Seed Germination ◽

Seed Quality ◽

Botanical Garden ◽

Soil Conditions ◽

Maximum Weight ◽

The South ◽

Laboratory Conditions ◽

Germination Energy

The morphology and sowing qualities of 7 types of hydrangea from the collection of the South Ural Bo-tanical Garden-Institute of the UFIC RAS were studied. The studies were carried out according to stand-ard methods. Is determined the mass of 1000 pieces seeds of each species, seed quality (germination en-ergy and germination in laboratory and soil conditions), the nature of seed germination, as well as their sowing qualities after different storage periods. It was found that the representatives of the Heteromallae subsection are characterized by heavier and larger seeds (the maximum weight of 1000 pieces of seeds is 0.06-0.09 g) compared with the representatives of the Americanae subsection (weight of 1000 pieces is 0.02 g). In addition, representatives of the Heteromallae subsection, with the exception of H. paniculata, showed consistently high rates of germination (72.3-85.8%) and germination energy (63.3-78.1%) under laboratory conditions; with soil sowing, these indicators are also significantly higher than that of repre-sentatives of the Americanae subsection.

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Kajian Pengusangan Cepat dan Penyimpanan Biji terhadap Perkecambahan Anaphalis longifolia (Blume) Blume ex.DC.

Jurnal Ilmu Kehutanan ◽

10.22146/jik.46144 ◽

2019 ◽

Vol 13 (1) ◽

pp. 48

Author(s):

Muhammad Imam Surya ◽

Suluh Normasiwi

Keyword(s):

Seed Germination ◽

Immersion Time ◽

Accelerated Aging ◽

Seed Storage ◽

Botanical Garden ◽

Conservation Value ◽

Limited Information ◽

Viability Test ◽

Seed Storability

Anaphalis longifolia merupakan kelompok bunga edelweiss yang memiliki nilai konservasi tinggi, namun upaya konservasi melalui kegiatan penyimpanan biji dan pengembangan usaha pembudidayaannya relatif masih terbatas. Penelitian ini melaporkan hasil dua percobaan yang dilaksanakan di laboratorium Kebun Raya Cibodas. Dalam percobaan pertama, pengusangan biji dilakukan menggunakan etanol 96% dengan 11 lama waktu perendaman (0, 10, 20, 30, 40, 50, 60, 70, 80, 90 dan 100 menit), sedangkan dalam percobaan kedua dilakukan pengujian kualitas daya simpan biji terhadap beberapa tempat penyimpanan seperti desikator, lemari, kulkas 4 oC dan freezer -20 oC selama kurun waktu 12 bulan. Hasil percobaan pertama menunjukkan bahwa perendaman biji pada etanol 96% selama 10-30 menit mampu memacu perkecambahan biji A. longifolia. Penyimpanan biji A. longifolia untuk waktu yang lama direkomendasikan menggunakan freezer dengan suhu -20 oC.A Study of Accelerated Aging and Seed Storage on the Germination of Anaphalis longifolia (Blume) Blume ex.DC. AbsractAnaphalis longifolia is a group of edelweiss flowers which has a highly conservation value. However, there are only limited information on conservation activities of A. longifolia regarded to the seed storage and cultivation. The study was conducted in the laboratory of Cibodas Botanical Garden. In this study, two experiments were carried out. In the first experiment, A. longifolia seeds were treated by ethanol 96% with 11 different immersion time (0, 10, 20, 30, 40, 50, 60, 70, 80, 90 and 100 minutes). In the second experiment, seed storability and viability test were conducted after A. longifolia seeds were saved in desiccator, cabinet, refrigerator 4 oC and freezer -20 oC during 12 months. Results of the first experiment showed that soaking seeds on 96% ethanol for 10-30 minutes was able to stimulate seed germination of A. longifolia. Freezer with temperature -20 oC is recommended to storage A. longifolia for long periods.

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Peningkatan Gelombang Elektromagnetik Menurunkan Laju Perkecambahan Padi Beras Merah Cenana Jatiluwih (Oryza Sativa Var Barac Cenana)

Jurnal BETA (Biosistem dan Teknik Pertanian) ◽

10.24843/jbeta.2018.v06.i02.p07 ◽

2018 ◽

Vol 6 (2) ◽

pp. 106

Author(s):

Viqtor Arya Nugraha ◽

I Made Anom S. Wijaya ◽

I Wayan Widia

Keyword(s):

Electromagnetic Field ◽

Oryza Sativa ◽

Seed Germination ◽

Electromagnetic Fields ◽

Immersion Time ◽

Red Rice ◽

Rice Varieties ◽

Local Varieties ◽

Two Factors ◽

Proper Immersion

Penelitian ini bertujuan untuk mengetahui pengaruh pemaparan medan elektromagnetik terhadap padi varietas lokal serta mengetahui medan elektromagnetik dan waktu perendaman yang tepat sehingga menghasilkan perkecambahan padi varietas lokal yang terbaik. Rancangan percobaan yang digunakan adalah Rancangan faktorial yang terdiri dari dua faktor. Faktor yang pertama yaitu kuat medan elektromagnetik dari 0 mT, 2 mT, 3 mT dan 4 mT, sedangkan faktor kedua yaitu lama perendaman terdiri dari 48, 64,dan 96 jam. Data yang diperoleh dianalisis dengan sidik ragam, kemudian dilanjutkan dengan uji duncan apabila perlakuan berpengaruh nyata. Hasil penelitian menunjukkan bahwa semakin kuat paparan elektromagnetik dengan tegangan tertentu mengakibatkan adanya penurunan terhadap persentase perkecambahan benih padi, sedangkan untuk perlakuan perendaman, semakin lama perendaman benih menghasilkan peningkatan persentase perkecambahan padi. Perkecambahan padi terbaik dihasilkan pada pemaparan medan elektromagnetik tegangan 2 mT dengan lama perendaman 96 jam. This study aims to find out the modeling of electromagnetic fields against local rice varieties and to determine the electromagnetic field and the proper immersion time to produce the best rice germination. The experimental experiment used is a factorial circuit consisting of two factors. The first factor is the strength of the electromagnetic field of 0 mT, 2 mT, 3 mT and 4 mT, while the second factor is the duration of immersion consisting of 48, 64, and 96 hours. The data obtained were analyzed by verbal examination, then continued with duncan test if treatment had real effect. The results showed that the stronger electromagnetic exposure with certain voltage resulted in a decrease in the percentage of seed germination of local varieties of rice while for the immersion treatment, the longer the seed immersion resulted in increased percentage of germination of local varieties of rice. The best germination was produced at exposure of 2 mT voltage electromagnetic field with 96 hours of immersion time for red rice varieties.

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Phytotoxic assessment of soils contaminated by railway transport facilities

Biological Systems: Theory and Innovation ◽

10.31548/biologiya2021.03.004 ◽

2021 ◽

Vol 12 (3) ◽

Author(s):

M Savchuk ◽

◽

O Sorochinska ◽

A Tsiko ◽

O Pilipchuk ◽

...

Keyword(s):

Seed Germination ◽

Anthropogenic Pollution ◽

Soil Samples ◽

Railway Transport ◽

Railway Line ◽

Railway Tracks ◽

Germination Energy ◽

Germination And Growth ◽

The City

The paper presents results of the study phytotoxicity level of soil that are contaminated due to the operation of the railway. The urgency of the work is determined by the growth of man-made load on the environment by rail. The aim of the presented work was to assess the phytotoxicity of soils near railway tracks using a bioassay for watercress to determine the level of their anthropogenic pollution. The object of the study were soil samples taken in the area adjacent to the tracks on the section of the railway Bakhmach-Hrebinka near the station of the city Pyriatyn. Soil sampling was carried out in accordance with DSTU ISO 10381-1: 2004. Assessment of soil contamination was assessed by bioindication using watercress test. According to the results of the research, it was found that the germination energy, seed germination and growth rates of watercress plants decreased on soil samples taken closer to the railway tracks. Weak levels of contamination were observed on soils selected at a distance of 5 meters from the railway, germination energy and seed germination were - 58 and 77%, respectively, the remaining samples did not show a significant effect on the sowing quality of watercress seeds. It was found that in plants grown on sample 4 (100 meters from the railway tracks) the length of the root system was 46 mm, while in samples 1, 2, 3 the indicator decreased respectively by 50, 32 and 9%, . The aerial part of watercress plants grown on 4 samples was 42 mm, while in samples 1,2,3, the figure decreased respectively by 43, 9 and 2%, . Therefore, it can be argued that the soil samples taken in the area adjacent to the tracks on the railway line Bakhmach-Grebinka, at a distance of 5 meters from the tracks, are low-contaminated, while the rest of the samples according to research are unpolluted and meet sanitary requirements on planning and development of settlements.

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