OXIC-HYGIENIC STUDY OF THE USE OF A SYNBIOTIC SUSPENSION AND A PROBIOTIC MODIFIED GINGIVAL GEL ON ANIMALS

2021 ◽  
pp. 44-50
Author(s):  
Наталия Владимировна Чиркова ◽  
Алена Алексеевна Плутахина ◽  
Ксения Евгеньевна Чиркова ◽  
Елена Анатольевна Андреева ◽  
Елена Юрьевна Каверина
Keyword(s):  
High Frequency ◽  
Antibacterial Agents ◽  
Periodontal Diseases ◽  
Age Groups ◽  
Blood Parameters ◽  
Internal Organs ◽  
Toxicological Study ◽  
Experimental Animals ◽  
White Rats ◽  
Weight Coefficients

Лечение заболеваний пародонта представляет собой одну из наиболее сложных и важных проблем современной стоматологии, актуальность которой обусловлена высокой частотой воспалительных заболеваний пародонта среди населения во всех возрастных группах - до 90% в популяции. Нерегулярность профилактики и эпизодические обращения к врачу - пародонтологу для лечения заболеваний пародонтита утяжеляют течение заболевания, которое при отсутствии лечения прогрессирует. Развитие пародонтита является результатом дисбаланса между микрофлорой полости рта и иммунной защитой организма. С целью ликвидации воспаления применяют различные иммунокорректоры и антибактериальные средства (антисептики, антибиотики, фитопрепараты). Альтернативой антибактериальной терапии при заболеваниях пародонта являются различные методы биотерапевтического воздействия, предполагающие местное и системное применение синбиотиков и пробиотиков, фаговых препаратов и других средств. Токсико - гигиенические исследования использования суспензии синбиотика «Бифистим», а также геля «Асепта с прополисом», модифицированного пробиотиком «Бифилиз» проводились на базе НИИ ВГМУ им. Н.Н. Бурденко. Была проведена оценка в остром и хроническом токсикологических экспериментах на 50 белых крысах (самцах) и на 5 морских свинках. Изучались кожно-резорбтивное действие, кумулятивные свойства, динамика массы и температуры тела, весовые коэффициенты внутренних органов, исследование показателей периферической крови. Оценка проведенных результатов токсикологического исследования опытных животных подтвердила отсутствие токсического действия исследуемой суспензии синбиотика «Бифистим», а также геля для десен «Асепта с прополисом», модифицированного пробиотиком «Бифилиз». Данные исследования позволили сделать вывод, что изучаемые препараты не токсичны и могут использоваться для лечения пациентов Treatment of periodontal diseases is one of the most difficult and important problems of modern dentistry, the relevance of which is due to the high frequency of inflammatory periodontal diseases among the population in all age groups - up to 90% in the population. Irregular prophylaxis and occasional visits to a periodontist for the treatment of periodontitis complicate the course of the disease, which progresses in the absence of treatment. The development of periodontitis is the result of an imbalance between the microflora of the oral cavity and the body's immune defenses. In order to eliminate inflammation, various immunocorrectors and antibacterial agents (antiseptics, antibiotics, phytopreparations) are used. An alternative to antibiotic therapy for periodontal diseases are various methods of biotherapeutic action, involving the local and systemic use of probiotics, phage preparations and other agents. Toxic and hygienic studies of the use of a suspension of sinbiotics "Bifistim" and as well as the gel "Asepta with propolis" modified with the probiotic "Bifiliz" were carried out on the basis of the Research Institute of VSMU them. N.N. Burdenko. Evaluation was carried out in acute and chronic toxicology experiments on 50 white rats (males) and 5 guinea pigs. Studied skin-resorptive effect, cumulative properties, dynamics of body weight and temperature, weight coefficients of internal organs, study of peripheral blood parameters. Evaluation of the results of the toxicological study of experimental animals confirmed the absence of the toxic effect of the investigated suspension of sinbiotics "Bifistim" as well as the gel for gums "Asepta with propolis", modified by the probiotic "Bifiliz". These studies led to the conclusion that the studied drugs are not toxic and can be used to treat patients

scholarly journals The occurrence of Entamoeba gingivalis among patients with periodontal disease

2016 ◽  
Vol 29 (2) ◽  
pp. 86-89 ◽  
Author(s):  
Joanna Luszczak ◽  
Michal Bartosik ◽  
Jolanta Rzymowska ◽  
Agnieszka Sochaczewska-Dolecka ◽  
Ewa Tomaszek ◽  
...  
Keyword(s):  
Oral Cavity ◽  
Periodontal Disease ◽  
Periodontal Diseases ◽  
Age Groups ◽  
Healthy People ◽  
Gingival Tissue ◽  
Pathological Changes ◽  
Oral Diseases

AbstractAccording to some studies, the Entamoeba gingivalis colonizing the gingival tissue is an important agent in bringing about periodontitis. Other studies, however, deem it an opportunist that is able to survive in the medium induced by periodontal disease. The aim of this study was to investigate the prevalence of Entamoeba gingivalis infection in patients from the Department of Periodontology, and compare this population with that of healthy people, so as to analyze the relationships between infection and patient sex and age. The result of this work is that in both groups, a correlation (p = 0,19) has been noted between the occurrence of amoebae and other diseases in the oral cavity. Indeed, 81,4% of all patients with some periodontal disease showed the presence of amoeba. Among those who are not afflicted with oral diseases, the presence of amoeba was indicated in 62,5% of the total. In addition, a correlation between the person's age and the presence of protozoa (p = 0,15) was strongly marked among women (p = 0,19). In the three age groups of women in this study (40-49, 60-69, and above 80 years), we observed a 100% presence of protozoa.Our study leads us to the conclusion that infections with Entamoeba gingivalis should be regarded as an factor that is associated with the pathological changes occurring in patients with periodontal diseases.

The Effect of Sodium Nitrite on Experimental Animals

1942 ◽  
Vol 6a (1) ◽  
pp. 63-73 ◽  
Author(s):  
H. L. A. Tarr ◽  
N. M. Carter
Keyword(s):  
Sodium Nitrite ◽  
Lethal Dose ◽  
Oral Route ◽  
Female Rats ◽  
Male Rats ◽  
Subcutaneous Route ◽  
Healthy Male ◽  
Experimental Animals ◽  
White Rats ◽  
Healthy Female

Incorporation of sodium nitrite in the diet of cats and white rats on the basis of an average sized man consuming 1 lb. (454 g.) of fish containing 0.2 per cent (908 mg.) of this salt daily for six days each week does not appear to affect their growth rate nor the development (weight) of their thyroid, heart, lungs, spleen, liver, kidneys or adrenals. The fecundity of white rats as judged by their ability to breed and raise normal litters is apparently not affected thereby. The lethal dose of sodium nitrite by oral route is about 1.1 to 2.0 g./kg. for healthy male rats, 0.46 to 1.2 g./kg. for healthy female rats and 0.073 g./kg. for cats (one animal). The lethal dose by subcutaneous route is about 0.19 to 0.20 g./kg. for healthy male rats and 0.057 to 0.13 g./kg. for healthy female rats.

EVALUATION OF TOXIOLOGICAL INDICATORS OF MONTHMORRILLONITE CLAY FOR THE PURPOSE OF USING THEM AS ALUMOSILICATE SOBENT AGAINST THE NEGATIVE EFFECT OF MYCOTOXINS ON THE BODY OF ANIMALS AND BIRDS

2021 ◽  
Vol 1 (1) ◽  
pp. 72-78
Keyword(s):  
Experimental Studies ◽  
The Body ◽  
Montmorillonite Clay ◽  
Test Substance ◽  
Negative Effects ◽  
Experimental Animals ◽  
Eyelid Edema ◽  
White Rats ◽  
Mucous Membranes ◽  
Negative Effect

The article presents the results of laboratory studies to assess the toxicological parameters of montmorillonite clay from the Pogadaevskoye deposit in the West Kazakhstan region in order to use them as an aluminosilicate sorbent in the composition of feeds that reduce the negative effects of mycotoxins on the body of animals and birds. The relevance of research is associated with the cultivation of healthy and highly productive animals and poultry in order to ensure the food security of the Republic of Kazakhstan.The studies carried out to assess the toxicological parameters of montmorillonite clay in order to use them as an aluminosilicate sorbent in experimental animals (rabbits and white rats) allowed the following results to be obtained: Visual study of the intensity of erythema when exposed to the test substance on the skin of rabbits showed their absence (0 points). The study of the intensity of edema (an increase in the thickness of the skin clutch of rabbits) when exposed to the test substance on the skin of rabbits showed no reaction (0 points). Studies evaluating the irritating effect of the test substances on the mucous membranes of the eyes of rabbits by symptoms of damage showed the absence of hyperemia (0 points). Weak eyelid edema (1 point), the minimum amount of discharge in the corner of the eye (1 point). The results of studies on the classification assessment of the test substance for the severity of the irritant effect on the eyes of rabbits showed that the average total score of the severity of the irritative effect corresponds to 1 point. A comprehensive analysis of the results obtained on the basis of scientific and experimental studies to assess the toxicological indicators of montmorillonite clay from the Pogadaevskoye deposit in relation to irritating effects on the skin and mucous membranes of experimental animals (rabbits) showed their harmlessness.

scholarly journals EFFECT OF UNILATERAL NEPHRECTOMY ON KIDNEY FUNCTION IN FERAL DOMESTIC CATS (Felis catus)

2019 ◽  
Vol 7 (2) ◽  
pp. 101-107
Author(s):  
Dionesio A. Estopa
Keyword(s):  
Kidney Function ◽  
Block Design ◽  
Age Groups ◽  
Unilateral Nephrectomy ◽  
Domestic Cats ◽  
Experimental Animals ◽  
Operative Care ◽  
Significant Difference ◽  
Randomized Complete Block Design ◽  
One Year

This experimental study was conducted to determine the effect of unilateral nephrectomy on kidney function through hematological and urological values after surgery using feral domestic cats.  Four (4) apparently healthy stray male cats were caught and used as experimental animals in this study. The animals were grouped into two (2) with two cats in each group. Grouping of the animals was based according to age, the 1st group – ages one year and above (>1 year) and the 2nd group – ages one year and below (<1 year).  A split plot randomized complete block design was used in the experiment. The result shows that rectal temperature and packed cell volume have been affected on both age group of cats and other urological values like the presence of urobilinogen, protein, leukocytes, erythrocytes and crystals were elevated twenty four hours after surgery. A significant increase on the level of creatinine and blood urea nitrogen has been noted from the 3rd and 15th post – operative day. However, no significant difference was noted between experimental animals and the two age groups, all of which have similar result. The findings of the study revealed that the remaining kidney could not completely compensate the function of the other kidney up to the 15th post – operative day, it may take longer than fifteen days. Hence, proper supportive treatment, post-operative care & management of animals subjected to unilateral nephrectomy is a must and should continue beyond fifteen days.

scholarly journals Distribution of Acid-fast Bacilli in the Skin, Extremities and Internal Organs of Various Experimental Animals

Repura ◽  
1966 ◽  
Vol 35 (1) ◽  
pp. 27-32
Author(s):  
TATSUO MORI ◽  
KENJI KOHSAKA ◽  
YOSHIHARU KISHI ◽  
MASAXO KAMEI ◽  
SHINJI NISHIMURA
Keyword(s):  
Internal Organs ◽  
Experimental Animals ◽  
Acid Fast Bacilli

scholarly journals Toxicity assessment of a technical product of the triazole class

2020 ◽  
Vol 99 (11) ◽  
pp. 1276-1279
Author(s):  
Valery N. Rakitskii ◽  
Tatiana M. Epishina ◽  
Elena G. Chkhvirkiya
Keyword(s):  
Body Weight ◽  
The Body ◽  
Male Rats ◽  
Acute Oral Toxicity ◽  
Oral Toxicity ◽  
High Biological Activity ◽  
Experimental Animals ◽  
Technical Product ◽  
White Rats ◽  
Toxicological Studies

Introduction. Historically, pesticides are evaluated more strictly from a medical point of view than other chemicals. Since their features, such as deliberate introduction into the environment, the possibility of contact with them by large masses of the population, and the high biological activity determine their potential danger to humans. Purpose of research - study of the biological effect of a technical product derived from triazoles when it is repeatedly ingested orally in mammals (rats), establishment of inactive and active doses, justification of the permissible daily dose (DSD) for humans. Material and methods. In acute experiments, white rats were used, including 6 animals in the group. Tested dose: 500-4000 mg/kg of body weight. A chronic (12 months) experiment was performed on 80 male rats with a bodyweight of 180-190 g at the beginning of the study. Tested doses: 5.0; 16.0 and 55.0 mg/kg of body weight (1 control and 3 experimental animals, 20 individuals each). In the dynamics of the experiment, we observed the condition and behavior of animals, water, and food consumption, recorded the timing of death, changes in body weight, physiological, biochemical, and hematological indices. Results. Indices of the acute oral toxicity on the studied product LD50 male rats were 2250 ± 483 mg/kg body weight. The dose of 5.0 mg / kg of body weight was not found to cause significant changes in all studied indices. The doses of 16.0 and 55.0 mg/kg of body weight had a polytropic effect on the body in experimental animals. Discussion. The studied product for the acute oral toxicity refers to low-hazard compounds, the doses of 16.0 and 55.0 mg/kg of body weight has a polytropic effect on the mammalian body, causing changes in carbohydrate, lipid, and lipoprotein metabolism in the body of rats - was accepted as acting. The dose of 5.0 mg / kg of body weight, when administered in rats, there are no changes in all the studied parameters throughout the experiment, is accepted as invalid. Based on the inactive dose-5.0 mg/kg of body weight and taking into account the reserve factor of 100, we have scientifically justified DSD for a person at the level of 0.05 mg/kg. Summary. The conducted sanitary and Toxicological studies indicate the need to assess the toxicity of new technical products to the mammalian body, to increase the reliability of the developed hygiene standards in environmental objects and food products.

scholarly journals Levels of Blood Glucose in Animals Try Wistar White Mix (Rattus Norvegicus) Dined by Green Beans of Sari Bean Packaging

2019 ◽  
Vol 4 (2) ◽  
pp. 17
Author(s):  
Maruni Wiwin Diarti ◽  
Siti Zaetun ◽  
Urip . ◽  
Yunan Jiwintarum ◽  
Baiq Anies Trisnasanti
Keyword(s):  
Blood Glucose ◽  
Functional Food ◽  
Green Bean ◽  
Green Beans ◽  
Experimental Animals ◽  
High Fiber ◽  
Glucose Levels ◽  
White Rats ◽  
Blood Glucose Levels ◽  
Reduce Blood Glucose

Functional food is food that is naturally or through a process that is beneficial to health. One functional food is a green bean juice packaging beverage that can reduce blood glucose levels. High fiber content, low glycemic index and flavonoids, and polyphenols in green beans (Phaseolus radiatus L) can reduce glucose levels in the blood. This study used experimental animals 7 tails. All experimental animals were given packaged mung bean juice 2 times a day for 9 days by means of a round. The mean results of an examination of blood glucose levels in white rat experimental animals before giving packaged green bean juice drinks was 85 mg dl, while the average results of an examination of blood glucose levels in experimental rats after administration of packaged green bean juice were 75 mg/dl. The results of the Paired T-test statistical test obtained p = 0,000 <α = 0.05. Packaged green bean juice can reduce blood glucose levels in animals testing white rats.

scholarly journals THE EFFECTIVENESS OF APHARM A COLOGICAL COMPOUND BASED ON SILATIVIT AND PERCUTANEO US ELECTRONE UROSTIMUL ATION AFTER LASER CURET TAGE IN PATIENTS WITH CHRONIC PERIODONTITIS

2013 ◽  
Vol 9 (1) ◽  
pp. 25-28
Author(s):  
Е. Светлакова ◽  
E. Svetlakova ◽  
Ю. Мандра ◽  
J. Mandra ◽  
Н. Жегалина ◽  
...  
Keyword(s):  
Chronic Periodontitis ◽  
High Frequency ◽  
Periodontal Diseases ◽  
Frequency Of Occurrence ◽  
Actual Problem ◽  
Dental Lasers ◽  
Percutaneous Electroneurostimulation

<p>Nowadays the treatment of periodontal diseases is an actual problem, that is caused by high frequency of occurrence of periodontopathies. diode dental lasers are nowadays often used for treatment periodontal pockets. Using hydrogel based on silativit and percutaneous electroneurostimulation healing of the wound surfaces.</p><p> </p><p> </p>

To the doctrine of the intravital coloration of the reticuloendothelial system in experimental animals

1930 ◽  
Vol 26 (4) ◽  
pp. 438-438
Author(s):  
L. I. Vilensky
Keyword(s):  
Connective Tissue ◽  
Reticuloendothelial System ◽  
The Body ◽  
Experimental Animals ◽  
White Rats ◽  
Medical Societies ◽  
Physiological Properties ◽  
Reticulo Endothelial System

Meetings of medical societies. Society of Physicians at Kazan University. General Sessions Session 4 March 1930 L.I. Vilensky. Towards the doctrine of the intravital coloration of the reticuloendothelial system in experimental animals. On the basis of a study of 267 animals of various species (rabbits, rats, pigs, white mice, white rats and dogs) the vivo coloring of trypanblau, pyrrholblau, carmine and collargol, the author comes to the following conclusions: 1) All active cellular elements of the indifferent connective tissue that has retained the embryonic properties of the mesenchyme. 2) Teaching-Aschoff's, in the light of intravital coloration, should be expanded, as the teaching of an active mesenchyme, scattered throughout the body, but united by common physiological properties. 3) The process of gradual absorption of colloidal paint in individual elements of the reticulo-endothelial system has a certain pattern, which is exactly the same in different animals.

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