In-laboratory quality control of nutrients for automatic bacteriology analyzer YUNON®Labstar 50

2021 ◽  
Vol 66 (2) ◽  
pp. 110-114
Author(s):  
Lyubov Grigorievna Boronina ◽  
E. V. Samatova ◽  
M. P. Kukushkina ◽  
S. A. Panova ◽  
S. S. Ustyugova
Keyword(s):  
Haemophilus Influenzae ◽  
Streptococcus Pyogenes ◽  
Nutrient Medium ◽  
Culture Media ◽  
Enterobacter Cloacae ◽  
Biological Properties ◽  
Nutrient Media ◽  
Biological Technology ◽  
Reference Strains

The quality of culture media for blood culture was checked: nutrient medium for children with an antibiotic neutralizer for the cultivation of aerobes, nutrient medium with an antibiotic neutralizer for the cultivation of anaerobes, a nutrient medium with an antibiotic neutralizer for the cultivation of aerobes, nutrient medium for the cultivation of aerobes UNONA® used in the automatic bacteriological analyzer JUNONA ®Labstar 50 (SCENKER Biological Technology Co., Ltd. China). Used tenfold dilutions from 18-24 hour cultures of reference strains: ATCC 13124 Clostridium perfringens; ATCC 25285 Bacteroides fragilis; NCTC 194I8 Haemophilus influenzae; ATCC 49619 Streptococcus pneumoniae; ATCC 16615 Streptococcus pyogenes; ATCC 27853 Pseudomonas aeruginosa; ATCC 25923 Staphylococcus aureus; ATCC 25922 Escherichia coli; BKPGU-401/-885-653 Candida albicans; ATCC13813 Streptococcus agalactiae; No. 186 Enterobacter cloacae; ATCC 29212 Enterococcus faecalis; clinical isolates: Acinetobacter lwofii, Enterobacter cloacae, Candida tropicalis. All investigated reference strains were isolated on nutrient media in accordance with their biological properties when inoculated with 50 CFU / ml less than 72 hours later, as stated by the manufacturer. The study has shown that growth factors must be used to test the quality of the culture media with Haemophilus influenzae bacteria and this must be reflected in the manufacturer’s instructions.

Comparative evaluation of nutrient media of Escherichia coli isolating for using in veterinary laboratories

Bacteriology ◽  
2020 ◽  
Vol 5 (2) ◽  
pp. 24-32
Author(s):  
Yu.A. Skomorina ◽  
◽  
A.A. Kremleva ◽  
L.Sh. Akhmetova ◽  
T.V. Podolskaya ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Key Words ◽  
Comparative Studies ◽  
Comparative Evaluation ◽  
Culture Media ◽  
Biological Properties ◽  
Nutrient Media ◽  
High Productivity ◽  
New Generation

The article is focus on comparative studies of modern differential diagnostic nutrient media for effective cultivation and identification of Escherichia coli. The assessment of the quality of media by biological properties was carried out in accordance with their purpose. Special attention is paid to the new generation of differential diagnostic nutrient media. It was found that the tested media have high productivity and inhibitory properties. New culture media of inland producers are not inferior in terms of the characteristics declared by the manufacturers. In case of methods validation the veterinarian laboratory will be able to use new nutrient media. Key words: nutrient media, Escherichia, productivity, selectivity and specificity

Comparative analysis of nutrient media for determination of the biochemical properties of Enterobacteria

Bacteriology ◽  
2020 ◽  
Vol 5 (1) ◽  
pp. 41-47
Author(s):  
O.V. Polosenko ◽  
◽  
A.P. Shepelin ◽  
Keyword(s):  
Culture Media ◽  
Biological Properties ◽  
Biochemical Properties ◽  
Comparative Assessment ◽  
Nutrient Media ◽  
Important Condition ◽  
Phenotypic Properties ◽  
Primary Isolation

The stage of identification of microorganisms is one of the most important and time-consuming stages of conducting bacteriological studies. On nutrient media with primary isolation by a combination of biochemical characteristics, several genera of enterobacteria can be detected simultaneously. Therefore, an important condition for the effectiveness of bacteriological studies to isolate representatives of the Enterobacteriaceae family is an adequate selection and quality of culture media. This allows you to determine the phenotypic properties of the grown cultures of microorganisms and correctly identify them. A comparative assessment of the biological properties of a number of modern nutrient media was carried out to determine the cultural-morphological and biochemical properties of enterobacteria. Key words: nutrient media, enterobacteria, phenotypic features, biochemical properties, differentiating properties, selectivity

scholarly journals The importance of alkalinity of culture media in mycology

2021 ◽  
Vol 25 (11) ◽  
pp. 1232-1232
Author(s):  
A. Dmitriev
Keyword(s):  
Nutrient Medium ◽  
Culture Media ◽  
Nutrient Media ◽  
The Difference

Kadisch (Dermat. Ztsch. Bd. 55, H 5/6, 1929), studying the importance of alkalinity of nutrient media in mycology, cultivated 30 varieties of various fungi, most of them pathogenic for humans, on media of different alkalinity and different composition. A. comes to the conclusion that fungi are cultivated best of all on alkalinity media Ph = 7, 2-7.6, the diameter of the colonies with it is the largest, the type of culture can be determined on the basis of the difference in the alkalinity of the nutrient medium.

Clinical trials of new culture media for staphylococcus isolation

2021 ◽  
Vol 66 (2) ◽  
pp. 115-121
Author(s):  
O. V. Polosenko ◽  
A. P. Shepelin ◽  
N. I. Azhermacheva ◽  
I. V. Abaev
Keyword(s):  
Growth Rate ◽  
Clinical Trials ◽  
Culture Media ◽  
Clinical Samples ◽  
Biological Parameters ◽  
Nutrient Media ◽  
Testing Laboratory ◽  
Statistical Reliability ◽  
And Control

A comparative analysis of the quality of the developed nutrient media, Baird-Parker dry agar base and Vogel-Johnson dry agar Base and foreign analogues, was done based on results of clinical trials. The tested media were qualified by the main biological parameters, such as sensitivity, growth rate, and differentiating and inhibiting properties. The evaluation of statistical reliability of the results of trials of clinical samples was evaluated taking into account the number of parallel studies and the number of matches of the results of studies conducted by different performers. 116 clinical samples of received by a laboratory of the testing laboratory center for research from hospital no.164 over the period of clinical trials were analyzed. 46 cultures of potential pathogens were isolated when culturing on test and control media: S. aureus -35; S. epidermidis-6; S. saprophyticus - 5. Lecithinase activity on the medium “Baird-Parker dry agar Base” and mannitol fermentation on the medium “Vogel-Johnson dry agar Base” in the preliminary phenotypic test allow the isolation and differentiation of clinical isolates of S. aureus from S. epidermidis, and S. saprophyticus.

Results of determination of the coli-titer in the semen of dogs when seeding on various nutrient media

2021 ◽  
Vol 1 (10) ◽  
pp. 42-46
Author(s):  
Seidfatima M. Borunova ◽  
◽  
Olga V. Karabanova ◽  
Ochir E. Badmaev ◽  
Anastasia V. Pomazkova ◽  
...  
Keyword(s):  
Nutrient Medium ◽  
Comparative Test ◽  
Coliform Bacteria ◽  
Pathogenic Microorganisms ◽  
Microbiological Method ◽  
Nutrient Media ◽  
Clinical Diagnostic ◽  
Diagnostic Center

The research is focused on the standardization of nutrient media while using the microbiological method of veterinary and sanitary control of the quality of dog sperm for the presence of pathogenic microorganisms, namely, coliform bacteria. 25 samples of frozen sperm of sire males were examined for a microbiological indicator (determination of bacteria coliform group). The samples were provided by the clinical diagnostic center FGBU «VGNKI». During the research a comparative test of Bulir's nutrient medium was carried out. Also were carried out tests for other nutrient media recommended for isolating coliform bacteria.

scholarly journals Adjustment of culture media to optimize in vitro rhizogenesis of domestic plums of various origins

2021 ◽  
Vol 34 ◽  
pp. 03001
Author(s):  
Natalia Kovalenko ◽  
Nadezhda Polivara
Keyword(s):  
Nutrient Medium ◽  
Culture Medium ◽  
Root Formation ◽  
Culture Media ◽  
Medium Composition ◽  
Ms Medium ◽  
Growth Substances ◽  
Nutrient Media ◽  
Plum Varieties

This paper presents the results of the adjustment of 18 modifications of culture medium based on MS medium composition – Murashige and Skoog (1962) for in vitro rhizogenesis of 10 varieties of domestic plum. There were used 4 nutrient media with a full amount of macro- and microelements – MS1 with a different combination of phytohormones, 1 medium without hormones – MSc, 13 media – MS2 with half the amount of macronutrients, differing in hormonal composition. It was found that the maximum number of rooted microshoots from 21.2 to 52.2 % was on MS2–8 medium, from 11.2 to 43.1 % on MS2–5. The analysis showed that into the medium increases the percentage of microplants by 12-17 % comparing with the medium MS2–5 and MS2–7 without FA. The distinctiveness of nutrient media by the type of auxin (IAA, NAA, IBA) made it possible to clarify that IBA is the most optimal of the auxins, and the concentration of 1.7 mg/l is borderline for the growth reactions of plum varieties. It was revealed that in vitro root formation depends not only on the compositions of growth substances in the nutrient medium, but also on the genotype of the variety.

scholarly journals Study of the Effect of a Biologically Active Compound Tris(2-hydroxyethyl)ammonium 4-Chlorophenylsulfanylacetate on the Growth of Listeria monocytogenes and Staphylococcus aureus

2020 ◽  
Vol 5 (1) ◽  
pp. 47-53
Author(s):  
S. V. Lukyanova ◽  
N. G. Gefan ◽  
S. N. Adamovich ◽  
E. N. Oborina ◽  
N. M. Khaptanova ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Growth Rate ◽  
Nutrient Medium ◽  
Biological Properties ◽  
Test Strain ◽  
Biologically Active ◽  
Nutrient Media ◽  
Growth Stimulator ◽  
Biologically Active Compound ◽  
Meat Peptone Agar

Background. Development of nutrient media ensuring the maximum growth rate of pathogens of dangerous infectious diseases while preserving their biological properties is extremely important. A promising direction in this area seems to be the use of synthetic microbial growth biostimulants.The aim of the work is to study the possibility of improving nutrient media for the cultivation of Listeria and Staphylococcus using a biologically active compound tris(2-hydroxyethyl)ammonium 4-chlorophenylsulfanylacetate.Materials and methods. The object of the study was experimental nutrient medium for the cultivation of Listeria used for the culturing of the test strain Listeria monocytogenes 766. As a comparison medium, commercial medium Fraser broth to which agar was added at a concentration of 1.5 %, was used. The test strain Staphylococcus aureus ATCC 6538-P (FDA 209-P) was cultivated on meat-peptone agar with 1% glucose. The compound tris(2-hydroxyethyl) ammonium (4-chlorophenyl)sulfanylacetate at a concentration of 10–4 wt. % was studied as a growth stimulator. A nutrient medium without a stimulant served as a control. The specific activity of nutrient media (germination rate, medium sensitivity, growth rate and stability of the main biological properties of microorganisms) was evaluated by the microbiological method.Results. Studies have shown that the addition of a growth stimulator to nutrient media contributes to the growth of colonies (by 10–50 %) and a decrease in the time of their development. When growth stimulator was added to the nutrient medium for the cultivation of Listeria, the initial growth of colonies of the L. monocytogenes 766 test strain after 12 hours of cultivation and growth of colonies of the test strain S. aureus ATCC 6538-P after 6 hours of cultivation on the meat-peptone agar with 1% glucose was observed.Conclusion. Thus, the addition of a growth biostimulator tris(2-hydroxyethyl)ammonium 4-chlorophenylsulfanyl acetate at a concentration of 10–4 wt. % in the nutrient medium accelerates the growth of Listeria and Staphylococcus, allows to reduce the time of issuance of the analysis result in half.

1,3-Thiazolidin-4-ones: Biological Potential, History, Synthetic Development and Green Methodologies

2018 ◽  
Vol 15 (8) ◽  
pp. 1109-1123
Author(s):  
Jonas da Silva Santos ◽  
Joel Jones Junior ◽  
Flavia M. da Silva
Keyword(s):  
Quality Of Life ◽  
Green Chemistry ◽  
Functional Group ◽  
Biological Properties ◽  
Structural Theory ◽  
Molecular Structures ◽  
Biological Potential ◽  
Synthetic Methodologies ◽  
Over Time

Background: We present here the synthesis of 1,3-thiazolidin-4-one (1) and its functionalised analogues, such as the classical isosteres, glitazone (1,3-thiazolidine-2,4-dione) (2), rhodanine (2-thioxo-1,3- thiazolidin-4-one) (3) and pseudothiohydantoin (2-imino-1,3-thiazolidin-4-one) (4) started in the midnineteenth century to the present day (1865-2018). Objective: The review focuses on the differences in the representation of the molecular structures discussed here over time since the first discussions about the structural theory by Kekulé, Couper and Butlerov. Moreover, advanced synthesis methodologies have been developed for obtaining these functional group, including green chemistry. We discuss about its structure and stability and we show the great biological potential. Conclusion: The 1,3-thiazolidin-4-one nucleus and functionalised analogues such as glitazones (1,3- thiazolidine-2,4-diones), rhodanines (2-thioxo-1,3-thiazolidin-4-ones) and pseudothiohydantoins (2-imino-1,3- thiazolidine-2-4-ones) have great pharmacological importance, and they are already found in commercial pharmaceuticals. Studies indicate a promising future in the area of medicinal chemistry with potential activities against different diseases. The synthesis of these nuclei started in the mid-nineteenth century (1865), with the first discussions about the structural theory by Kekulé, Couper and Butlerov. The present study has demonstrated the differences in the representations of the molecular structures discussed here over time. Since then, various synthetic methodologies have been developed for obtaining these nuclei, and several studies on their structural and biological properties have been performed. Different studies with regards to the green synthesis of these compounds were also presented here. This is the result of the process of environmental awareness. Additionally, the planet Earth is already showing clear signs of depletion, which is currently decreasing the quality of life.

Chitosan in Biomedical Engineering: A Critical Review

2019 ◽  
Vol 14 (2) ◽  
pp. 93-116 ◽  
Author(s):  
Shabnam Mohebbi ◽  
Mojtaba Nasiri Nezhad ◽  
Payam Zarrintaj ◽  
Seyed Hassan Jafari ◽  
Saman Seyed Gholizadeh ◽  
...  
Keyword(s):  
Quality Of Life ◽  
Biomedical Engineering ◽  
Biological Properties ◽  
Advanced Materials ◽  
Comprehensive Overview ◽  
Chemical Structures ◽  
Bio Imaging ◽  
Significant Attention ◽  
Unique Chemical

Biomedical engineering seeks to enhance the quality of life by developing advanced materials and technologies. Chitosan-based biomaterials have attracted significant attention because of having unique chemical structures with desired biocompatibility and biodegradability, which play different roles in membranes, sponges and scaffolds, along with promising biological properties such as biocompatibility, biodegradability and non-toxicity. Therefore, chitosan derivatives have been widely used in a vast variety of uses, chiefly pharmaceuticals and biomedical engineering. It is attempted here to draw a comprehensive overview of chitosan emerging applications in medicine, tissue engineering, drug delivery, gene therapy, cancer therapy, ophthalmology, dentistry, bio-imaging, bio-sensing and diagnosis. The use of Stem Cells (SCs) has given an interesting feature to the use of chitosan so that regenerative medicine and therapeutic methods have benefited from chitosan-based platforms. Plenty of the most recent discussions with stimulating ideas in this field are covered that could hopefully serve as hints for more developed works in biomedical engineering.

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