scholarly journals Study of the Effect of a Biologically Active Compound Tris(2-hydroxyethyl)ammonium 4-Chlorophenylsulfanylacetate on the Growth of Listeria monocytogenes and Staphylococcus aureus

2020 ◽  
Vol 5 (1) ◽  
pp. 47-53
Author(s):  
S. V. Lukyanova ◽  
N. G. Gefan ◽  
S. N. Adamovich ◽  
E. N. Oborina ◽  
N. M. Khaptanova ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Growth Rate ◽  
Nutrient Medium ◽  
Biological Properties ◽  
Test Strain ◽  
Biologically Active ◽  
Nutrient Media ◽  
Growth Stimulator ◽  
Biologically Active Compound ◽  
Meat Peptone Agar

Background. Development of nutrient media ensuring the maximum growth rate of pathogens of dangerous infectious diseases while preserving their biological properties is extremely important. A promising direction in this area seems to be the use of synthetic microbial growth biostimulants.The aim of the work is to study the possibility of improving nutrient media for the cultivation of Listeria and Staphylococcus using a biologically active compound tris(2-hydroxyethyl)ammonium 4-chlorophenylsulfanylacetate.Materials and methods. The object of the study was experimental nutrient medium for the cultivation of Listeria used for the culturing of the test strain Listeria monocytogenes 766. As a comparison medium, commercial medium Fraser broth to which agar was added at a concentration of 1.5 %, was used. The test strain Staphylococcus aureus ATCC 6538-P (FDA 209-P) was cultivated on meat-peptone agar with 1% glucose. The compound tris(2-hydroxyethyl) ammonium (4-chlorophenyl)sulfanylacetate at a concentration of 10–4 wt. % was studied as a growth stimulator. A nutrient medium without a stimulant served as a control. The specific activity of nutrient media (germination rate, medium sensitivity, growth rate and stability of the main biological properties of microorganisms) was evaluated by the microbiological method.Results. Studies have shown that the addition of a growth stimulator to nutrient media contributes to the growth of colonies (by 10–50 %) and a decrease in the time of their development. When growth stimulator was added to the nutrient medium for the cultivation of Listeria, the initial growth of colonies of the L. monocytogenes 766 test strain after 12 hours of cultivation and growth of colonies of the test strain S. aureus ATCC 6538-P after 6 hours of cultivation on the meat-peptone agar with 1% glucose was observed.Conclusion. Thus, the addition of a growth biostimulator tris(2-hydroxyethyl)ammonium 4-chlorophenylsulfanyl acetate at a concentration of 10–4 wt. % in the nutrient medium accelerates the growth of Listeria and Staphylococcus, allows to reduce the time of issuance of the analysis result in half.

scholarly journals Karalicin, a New Biologically Active Compound from Pseudomonas fluorescens/putida. II. Biological Properties.

1996 ◽  
Vol 49 (3) ◽  
pp. 263-266 ◽  
Author(s):  
GIORGIO LAMPIS ◽  
DELIA DEIDDA ◽  
CARLO MAULLU ◽  
SABRINA PETRUZZELLI ◽  
RAFFAELLO POMPEI ◽  
...  
Keyword(s):  
Pseudomonas Fluorescens ◽  
Active Compound ◽  
Biological Properties ◽  
Biologically Active ◽  
Biologically Active Compound

scholarly journals Cultivation of the diatom algae Chaetoceros calcitrans f. pumilus (Paulsen) Takano, 1968 as food for giant oyster larvae Crassostrea gigas (Thunberg)

2019 ◽  
Vol 4 (2) ◽  
pp. 34-40 ◽  
Author(s):  
L. V. Ladygina ◽  
A. V. Pirkova
Keyword(s):  
Growth Rate ◽  
Crassostrea Gigas ◽  
Nutrient Medium ◽  
Growth Parameters ◽  
Inorganic Nitrogen ◽  
Diatom Algae ◽  
Nitrogen And Phosphorus ◽  
Nutrient Media ◽  
Chaetoceros Calcitrans ◽  
Giant Oyster

An impact of modified nutrient media F/2 and Conway on the growth and biomass accumulation of the diatom algae Chaetoceros calcitrans f. pumilus, which is a part of the food for cultivated larvae of the giant oyster Crassostrea gigas in the IMBR RAS nursery, was studied. Maximum values of cell and biomass concentrations were obtained on the modified F/2 nutrient medium (11.22 × 106 cells·ml-1 and 4.93 g·l-1, respectively), and they were much larger than those obtained on Conway medium. Growth parameters of C. calcitrans f. pumilus depended on the ratio of inorganic nitrogen and phosphorus, as well as on the silicon content in nutrient media. The ratio N : P = 12.5 and the silicon concentration of 24 mg·l-1 in the modified F/2 nutrient medium are shown to be approaching the optimal ones for increasing growth rate of diatom algae. It is found that the microalga in concentration 150 × 103 cells·ml-1, cultivated on different nutrient media and included in food composition, has impact on the growth rate of giant oyster larvae. An average daily amount of growth of larvae, whose diet included algae cultivated on modified F/2 nutrient medium, was higher than that of larvae cultivated on Conway medium.

scholarly journals Comparative evaluation of hydrolysates as a basis for the construction of a nutrient medium for the cultivation of Listeria monocytogenes

2021 ◽  
Author(s):  
N. M. Khaptanova ◽  
А. S. Ostyak ◽  
S. V. Lukyanova ◽  
V. I. Kuznetsov ◽  
N. M. Аndreevskaya ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Enzymatic Hydrolysis ◽  
Nutrient Medium ◽  
Comparative Evaluation ◽  
Raw Materials ◽  
Amino Nitrogen ◽  
Test Strain ◽  
Nutrient Media ◽  
Microbiological Methods ◽  
The Common

The objective is to perform a comparative evaluation of the pancreatic hydrolysates prepared from fish and squid to determine the optimal culture medium for Listeria monocytogenes.Materials and methods. The following raw materials were used in the study: Pacific Herring (Clupea pallasii), Alaska Pollock (Gadus chalcogrammus), Common Roach (Rutilus rutilus lacustris), European Squid (Loligo vulgaris). The raw materials were subjected to enzymatic hydrolysis using the pancreas (according to Hottinger). A study of the physicochemical properties of pancreatic hydrolysates (content of free amino nitrogen (FAN), acidity of fish hydrolysates, the amino acid composition) was carried out.. The specific activity of nutrient media during the cultivation of the test strain L. monocytogenes 766 was assessed by a complex of microbiological methods.Results and discussion. The highest content of FAN at the end of enzymatic hydrolysis was observed in the pancreatic hydrolysate of the common roach (6%), the acidity of the hydrolysate remained stable from 6th to 13th day of the hydrolysis process (pH 7.2). Pancreatic hydrolysate of the common roach contained a number of amino acids that are most essential for the growth of Listeria. An assessment of the biological properties of nutrient media prepared on the basis of the obtained hydrolysates demonstrated that the best results in terms of sensitivity and germination of L. monocytogenes 766 showed a nutrient medium based on the pancreatic hydrolysate of the common roach. During the cultivation of L. monocytogenes 766 the test strain retained its morphological and cultural properties and did not show signs of dissociation.Conclusion. The research results have shown that the pancreatic hydrolysate of the common roach is a promising protein basis for the construction of an experimental environment for listeria. 

In-laboratory quality control of nutrients for automatic bacteriology analyzer YUNON®Labstar 50

2021 ◽  
Vol 66 (2) ◽  
pp. 110-114
Author(s):  
Lyubov Grigorievna Boronina ◽  
E. V. Samatova ◽  
M. P. Kukushkina ◽  
S. A. Panova ◽  
S. S. Ustyugova
Keyword(s):  
Haemophilus Influenzae ◽  
Streptococcus Pyogenes ◽  
Nutrient Medium ◽  
Culture Media ◽  
Enterobacter Cloacae ◽  
Biological Properties ◽  
Nutrient Media ◽  
Biological Technology ◽  
Reference Strains

The quality of culture media for blood culture was checked: nutrient medium for children with an antibiotic neutralizer for the cultivation of aerobes, nutrient medium with an antibiotic neutralizer for the cultivation of anaerobes, a nutrient medium with an antibiotic neutralizer for the cultivation of aerobes, nutrient medium for the cultivation of aerobes UNONA® used in the automatic bacteriological analyzer JUNONA ®Labstar 50 (SCENKER Biological Technology Co., Ltd. China). Used tenfold dilutions from 18-24 hour cultures of reference strains: ATCC 13124 Clostridium perfringens; ATCC 25285 Bacteroides fragilis; NCTC 194I8 Haemophilus influenzae; ATCC 49619 Streptococcus pneumoniae; ATCC 16615 Streptococcus pyogenes; ATCC 27853 Pseudomonas aeruginosa; ATCC 25923 Staphylococcus aureus; ATCC 25922 Escherichia coli; BKPGU-401/-885-653 Candida albicans; ATCC13813 Streptococcus agalactiae; No. 186 Enterobacter cloacae; ATCC 29212 Enterococcus faecalis; clinical isolates: Acinetobacter lwofii, Enterobacter cloacae, Candida tropicalis. All investigated reference strains were isolated on nutrient media in accordance with their biological properties when inoculated with 50 CFU / ml less than 72 hours later, as stated by the manufacturer. The study has shown that growth factors must be used to test the quality of the culture media with Haemophilus influenzae bacteria and this must be reflected in the manufacturer’s instructions.

scholarly journals Characteristics growth of mycelium of mushroom shiitake Lentinula Edodes (Berk.) Pegler in vitro

2019 ◽  
pp. 20-25
Author(s):  
T. Ivanova ◽  
N. Voloschuk
Keyword(s):  
Growth Rate ◽  
Light Microscopy ◽  
Pure Culture ◽  
Nutrient Medium ◽  
Sodium Selenite ◽  
Lentinula Edodes ◽  
Ph Value ◽  
Nutrient Media ◽  
Microbiological Methods

Goal. The purpose of this work is to study the growth of the mycelium of the fungus Lentinula Edodes (Berk.) Pegler on nutrient media of different composition and also to study the features of the use of Avatar-1 micro fertilizer and sodium selenite. Methods. Biotechnological research methods. Sodium selenite (Na2S eO3) at a concentration of 1.0 mmol / l was also used. Pure culture of the mushrooms had a very dense structure and white color. In our work, we used biotechnological methods — obtain this by subculturing the L. edodes strain in vitro; microbiological methods — obtaining pure culture of the fungus, the study of the cultural properties of the colonies. We set the pH value (pH) of nutrient media at the beginning and at the end of incubation. We applied mycological methods to measure the speed, density of growth and dry mass of mycelium. we used the light microscopy method. We performed statistical data processing.Method of light microscopy. Results. The experiments showed about acceleration of mycelial growth, mass and the greatest yield of mycelium L. edodes were on a nutrient medium with microfertilizer Avatar-1. In the experiment, it was found that the maximum overgrowth of the medium by mycelium occurs at 7 days. We have been proved that in the «Avatar-1» nutrient medium there was an increase and consolidation of bifurcated hyphae and buckles of L. edodes. There was even germination of mycelium that did not have too thin or thick hyphae. The dependence of growth rate on the type of nutrient medium. Also, the of doses of the drug, which effectively influences the technology of obtaining primary mycelium L. edodes. Conclusions. The dependence of the growth rate on the type of nutrient medium, the dose administration rate, as well as the cultivation regimes, that effectively influence and cheapen the technology of obtaining the primary mycelium L. edodes is demonstrated.

The Molecular Diversity Scope of Urazole in the Synthesis of Organic Compounds

2019 ◽  
Vol 16 (7) ◽  
pp. 953-967 ◽  
Author(s):  
Ghodsi M. Ziarani ◽  
Fatemeh Mohajer ◽  
Razieh Moradi ◽  
Parisa Mofatehnia
Keyword(s):  
Biological Activities ◽  
Biological Properties ◽  
Biologically Active ◽  
Microtubule Assembly ◽  
Heterocyclic Molecules ◽  
Biological Perspective ◽  
Highly Active ◽  
Anti Inflammatory ◽  
High Yields ◽  
Short Time

Background: As a matter of fact, nitrogen as a hetero atom among other atoms has had an important role in active biological compounds. Since heterocyclic molecules with nitrogen are highly demanded due to biological properties, 4-phenylurazole as a compound containing nitrogen might be important in the multicomponent reaction used in agrochemicals, and pharmaceuticals. Considering the case of fused derivatives “pyrazolourazoles” which are highly applicable because of their application for analgesic, antibacterial, anti-inflammatory and antidiabetic activities as HSP-72 induction inhibitors (I and III) and novel microtubule assembly inhibitors. It should be mentioned that spiro-pyrazole also has biological activities like cytotoxic, antimicrobial, anticonvulsant, antifungal, anticancer, anti-inflammatory, and cardiotonic activities. Objective: Urazole has been used in many heterocyclic compounds which are valuable in organic syntheses. This review disclosed the advances in the use of urazole as the starting material in the synthesis of various biologically active molecules from 2006 to 2019. Conclusion: Compounds of urazole (1,2,4-triazolidine-3,5-dione) are the most important molecules which are highly active from the biological perspective in the pharmaceuticals as well as polymers. In summary, many protocols for preparations of the urazole derivatives from various substrates in multi-component reactions have been reported from different aromatic and aliphatic groups which have had carbonyl groups in their structures. It is noted that several catalysts have been synthesized to afford applicable molecules with urazole scaffolds. In some papers, being environmentally friendly, short time reactions and high yields are highlighted in the protocols. There is a room to synthesize new catalysts and perform new reactions by manipulating urazole to produce biologically active compounds, even producing chiral urazole component as many groups of chiral urazole compounds are important from biological perspective.

scholarly journals Isobornylchalcones as Scaffold for the Synthesis of Diarylpyrazolines with Antioxidant Activity

Molecules ◽  
2021 ◽  
Vol 26 (12) ◽  
pp. 3579
Author(s):  
Svetlana A. Popova ◽  
Evgenia V. Pavlova ◽  
Oksana G. Shevchenko ◽  
Irina Yu. Chukicheva ◽  
Aleksandr V. Kutchin
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Properties ◽  
Biological Properties ◽  
High Reactivity ◽  
Biologically Active ◽  
Brain Lipids ◽  
Wide Range ◽  
Privileged Structure ◽  
Vitro Model

The pyrazoline ring is defined as a “privileged structure” in medicinal chemistry. A variety of pharmacological properties of pyrazolines is associated with the nature and position of various substituents, which is especially evident in diarylpyrazolines. Compounds with a chalcone fragment show a wide range of biological properties as well as high reactivity which is primarily due to the presence of an α, β-unsaturated carbonyl system. At the same time, bicyclic monoterpenoids deserve special attention as a source of a key structural block or as one of the pharmacophore components of biologically active molecules. A series of new diarylpyrazoline derivatives based on isobornylchalcones with different substitutes (MeO, Hal, NO2, N(Me)2) was synthesized. Antioxidant properties of the obtained compounds were comparatively evaluated using in vitro model Fe2+/ascorbate-initiated lipid peroxidation in the substrate containing brain lipids of laboratory mice. It was demonstrated that the combination of the electron-donating group in the para-position of ring B and OH-group in the ring A in the structure of chalcone fragment provides significant antioxidant activity of synthesized diarylpyrazoline derivatives.

scholarly journals The ameliorative effect of selenium on Azolla caroliniana grown under UV-B stress

2015 ◽  
Vol 95 (1) ◽  
pp. 20-26 ◽  
Author(s):  
E.M. Mostafa ◽  
A.M.A. Hassan
Keyword(s):  
Growth Rate ◽  
Protein Content ◽  
Doubling Time ◽  
The Other ◽  
Total Glutathione ◽  
Nutrient Media ◽  
Relative Growth ◽  
Other Hand ◽  
Ameliorative Effect ◽  
Glutathione Redox

Exposure ofAzollaplants to UV-B radiation for 6 h resulted in a decrease in biomass and relative growth rate (RGR), which coincided with an increase in doubling time (DT) as compared with the control. Also, the protein content decreased. On the other hand, hydrogen peroxyde (H2O2) and malondialdehyde (MDA) accumulated significantly in UV-treatedAzollaplants. Conversely, the addition of selenium (Se) at 1 ppm resulted in a significant increase in biomass and protein content of untreated and UV-treatedAzollaplants, and a significant reduction in both H2O2and MDA. Moreover, the addition of Se to UV-treated and untreatedAzollaplants resulted in a significant increase in total ascorbate and total glutathione (GSH) contents compared with the control and UV-stressedAzollaplants. Also, glutathione redox potential (GSH/TG) increased significantly in UV-treatedAzollaplants in the presence of Se. There also was a significant increase (38%) in ascorbate peroxidase (APX) activity in UV-treated plants compared with the control. APX activity in the presence of Se did not change significantly compared with the control. Glutathione reductase (GR) activity increased significantly in UV-treatedAzolla, while glutathione peroxidase (GSH-PX) activity did not. On the other hand, both GSH-PX and GR activity in untreated and UV-treatedAzollaplants were significantly enhanced by the application of Se to the nutrient media at a concentration of 1 ppm. Therefore, we can conclude that Se protectsAzollaplants from UV-B stress.

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