scholarly journals Blood profile of prevalent sheep breeds in Nigeria: A case study of Ikorodu Local Government Area of Lagos State, Nigeria

2021 ◽  
Vol 48 (5) ◽  
pp. 293-299
Author(s):  
F. P. Agbaye ◽  
A. O. Sokunbi ◽  
M. A. Onigemo ◽  
O. Alaba ◽  
O. A. J. Anjola ◽  
...  

The role of the environment in the survivability of an animal is paramount and blood profiles are part of the indices for measuring the health status. Therefore, a study was carried out to determine the blood profile of prominent indigenous breed of sheep (Ram) viz: West African Dwarf, Yankasa, Balami and Ouda breeds reared in Ikorodu Local Government. A total of 20 rams were used comprising of five rams per breed, with average age of 2½ years weighing 31.13±4.8 kg. Blood samples were carefully collected from the rams into labeled sterile bottles containing EDTA for haematological examination and another set of bottles containing Lithium Heparin was used to collect blood for the serum biochemical analysis. Haematological parameter assessed were Packed Cell Volume (PCV), White Blood Cell (WBC), Red Blood Cell (RBC), while the biochemical parameters investigated were Total protein (Tp), Albumin, Glucose, Creatine Cholesterol, and Alkaline phosphatase (ALP). PCV was significantly (p<0.05) highest (32.25%) in Yankassa and lowest (26.25%) in Ouda. Yankasa had statistically (p<0.05) the higher 11.66g/dL Hb, followed by WAD (10.00), Ouda (9.60) and lower (9.20) in Balami. RBC (x10/ml) was significantly (p<0.05) higher in WAD (3.72) than other breeds and lowest in Ouda (3.11). MCV (fl) value was significantly lower in Yankasa (75.83) than other breeds and higher in Ouda (83.60). MCH (pg) was statistically (p<0.05) highest (30.89) in Yankasa and lower (26.93) in Balami. Yankasa had significantly(p<0.05) the higher (8673.00) Total WBC (mm3), followed by WAD (8453.25), Balami (6814.25) and least in Ouda (6718.75). Total protein was significantly (p<0.05) the higher (53.76) in WAD ram and lower (43.76) in Ouda. Yankasa ram recorded significantly (p<0.05) the higher Albumin content (5.45), glucose level (48.87), ALP (129.71) and significantly the least creatine (0.63) while significantly (p<0.05) the least values for Glucose were in Ouda (44.83), Albumin content and Alkaline phosphatase in Balami (4.33, 104.87 respectively). Cholesterol value was significantly the higher (93.22) in WAD and lower (79.70) in Balami. Results revealed significant differences in haematological and serological indices tested though all values obtained were within the normal range for sheep.    Le rôle de l'environnement dans la survie d'un animal est primordial et les profils sanguins font partie des indices de mesure de l'état de santé. Par conséquent, une étude a été menée pour déterminer le profil sanguin de la race indigène de moutons (bélier), à savoir: les moutons nains d'Afrique de l'Ouest (WAD), Yankasa, Balami et Ouda élevés dans la collectivité locale d'Ikorodu. Au total, 20 béliers ont été utilisés comprenant cinq béliers par race, avec un âge moyen de 2 ans et demi pesant 31,13 ± 4,8 kg. Des échantillons de sang ont été soigneusement prélevés sur les béliers dans des flacons stériles étiquetés contenant de l'EDTA pour un examen hématologique et un autre ensemble de flacons contenant de l'héparine de lithium a été utilisé pour collecter du sang pour l'analyse biochimique du sérum. Les paramètres hématologiques évalués étaient le volume de cellules emballées (PCV), les globules blancs (WBC), les globules rouges (RBC), tandis que les paramètres biochimiques étudiés étaient la protéine totale (Tp), l'albumine, le glucose, la créatine cholestérol et la phosphatase alcaline (ALP). Le PCV était significativement (p <0,05) le plus élevé (32,25%) à Yankassa et le plus bas (26,25%) à Ouda. Yankasa avait statistiquement (p <0,05) le taux d'Hb le plus élevé de 11,66 g / dL, suivi de WAD (10,00), Ouda (9,60) et moins (9,20) à Balami. Les globules rouges (x10 / ml) étaient significativement (p <0,05) plus élevés chez WAD (3,72) que chez les autres races et plus faibles chez Ouda (3,11). La valeur MCV (fl) était significativement plus faible à Yankasa (75,83) que dans les autres races et plus élevée à Ouda (83,60). MCH (pg) était statistiquement (p <0,05) la plus élevée (30,89) à Yankasa et inférieure (26,93) à Balami. Yankasa avait significativement (p <0,05) le plus élevé (8673,00) WBC total (mm3), suivi des moutons nains d'Afrique de l'Ouest, (8453,25), Balami (6814,25) et inférieur à Ouda (6718,75). La protéine totale était significativement (p <0,05) la plus élevée (53,76) chez les moutons nains d'Afrique de l'Ouest, et la plus faible (43,76) chez Ouda. Le bélier Yankasa a enregistré de manière significative (p <0,05) la plus haute teneur en albumine (5,45), le niveau de glucose (48,87), l'ALP (129,71) et significativement le moins de créatine (0,63) tandis que de manière significative (p <0,05) les valeurs les plus faibles pour le glucose étaient à Ouda (44,83), teneur en albumine et phosphatase alcaline chez Balami (4,33, 104,87 respectivement). La valeur du cholestérol était significativement la plus élevée (93,22) chez les moutons nains d'Afrique de l'Ouest et la plus basse (79,70) chez Balami. Les résultats ont révélé des différences significatives dans les indices hématologiques et sérologiques testés bien que toutes les valeurs obtenues se situent dans la plage normale pour les moutons.

2021 ◽  
Vol 282 ◽  
pp. 03007
Author(s):  
V.I. Eremenko ◽  
Yu.I. Gatilova ◽  
A.V. Blednova ◽  
S.Yu. Steblovskaya ◽  
G.I. Shvets

Scientific and production experiments were carried out on lactating cows of the Simmental breed. 4 groups of cows derived from different lines of bulls Romulus, Redad, Huxle, and Honig were formed for the experiment. Each group contained 10 heads. The level of dairy productivity of experimental cows was the same and amounted to about 11 thousand kg of milk per lactation. The keeping conditions for the animals were the same. Feeding levels were consistent with their dairy productivity. Blood for the study was taken once a month prior to morning feeding. The following were determined in blood samples: total protein, alanine aminotransferase (ALT), aspartate aminotransferase (AAT), alkaline phosphatase (AP) and lactic dehydrogenase (LDH). During lactation, the highest level of total protein, the activity of ALT, AAT, AP, LDH was observed at the peak of lactation, followed by their decrease by the end of lactation regardless of cows’ genetic affiliation. Relatively higher levels of total protein and ALT, AAT and LDH activity have been observed in lactating cows of the bull line Romulus relative to cows of the bull lines Redad, Huxle and Honig. There were no differences within the cows’ genetic lines in the activity of alkaline phosphatase.


2005 ◽  
Vol 39 (3) ◽  
pp. 321-330 ◽  
Author(s):  
M Kitagaki ◽  
M Yamaguchi ◽  
M Nakamura ◽  
K Sakurada ◽  
T Suwa ◽  
...  

Age-related changes in haematology and serum chemistry values were examined in male and female Weiser–Maples guineapigs ( Cavia porcellus). Haematological changes that significantly ( P < 0.01) correlated with ageing were increased white blood cell and neutrophil counts in both sexes, decreased lymphocyte counts in both sexes, decreased reticulocyte and platelet counts in males, and decreased basophil counts in females. For serum chemistry, increases in total protein, triglycerides, blood urea nitrogen and creatinine were seen in both sexes, along with increases in total cholesterol in males and sodium in females. Decreased alkaline phosphatase in both sexes and decreased chloride in males were significantly ( P < 0.01) associated with age. These age-related changes are compared with the published literature.


Author(s):  
A. Jaiswal ◽  
S. S. Pandey ◽  
A. S. Parihar ◽  
N. Rajput ◽  
R. Jain

The study was conducted on 12 healthy male buffalo calves weighing between 50 to 60 Kg to evaluate haemato-biochemical alterations after epidural injection of dexmedetomidine alone and in combination with bupivacaine. Haematological examination showed nonsignificant alterations. Biochemical examination showed significant increase in blood glucose, blood urea nitrogen and serum creatinine while there was nonsignificant alterations in total protein and alkaline phosphatase. All haemato-biochemical changes were transient and compensatory.


2018 ◽  
Vol 46 (1) ◽  
Author(s):  
Nermin Isik ◽  
Ozlem Derinbay Ekici ◽  
Ceylan Ilhan ◽  
Devran Coskun

 Background: Theileriosis is a tick-borne disease caused by Theileria strains of the protozoan species. Buparvaquone is the mostly preferred drug in the treatment theileriosis, while it is safety in sheep, has not been detailed investigated. It has been hypothesized that buparvaquone may show side effects and these effects may be defined some parameters measured from blood in sheep when it is used at the recommended dose and duration. The aim of this research was to determine the effect of buparvaquone on the blood oxidative status, cardiac, hepatic and renal damage and bone marrow function markers.Materials, Methods & Results: In this study, ten adult (> 2 years) Akkaraman rams were used. Healthy rams were placed in paddocks, provided water ad libitum, and fed with appropriate rations during the experiment. Buparvaquone was ad­ministered at the dose of 2.5 mg/kg (IM) intramuscularly twice at 3-day intervals. Blood samples were obtained before (0. h, Control) and after drug administration at 0.25, 0.5, 1, 2, 3, 4 and 5 days. The blood samples were transferred to gel tubes, and the sera were removed (2000 g, 15 min). During the study, the heart rate, respiratory rate, and body temperature were measured at each sampling time. In addition, the animals were clinically observed. Plasma oxidative status mark­ers (Malondialdehyde, total antioxidant status, catalase, glutathione peroxidase, superoxide dismutase), serum cardiac (Troponin I, creatine kinase-MBmass, lactate dehydrogenase), hepatic (Alkaline phosphatase, alanine aminotransferase, aspartate aminotransferase, gamma glutamyltransferase, total protein, albumin, globulin) and renal (Creatinine, blood urea nitrogen) damage markers and hemogram values (white blood cell, red blood cell, platelet, hemogram, hematocrit) were measured. Buparvaquone caused statistically significantly (P < 0.05) increases in the troponin I and blood urea nitrogen levels and fluctuations in alkaline phosphatase activity, but there was no any statistically significance difference determined in the other parameters.Discussion: In this study, buparvaquone was administered two times at a dose of 2.5 mg/kg (IM) at 3-day intervals. Al­though the result was not statistically significant (P > 0.05), it was determined that buparvaquone gradually increased the levels of the main oxidative stress marker, MDA, by approximately 2.8 fold. CAT and GPX levels were also found to have decreased by 2.2 fold. Buparvaquone may cause lipid peroxidation by producing free radicals. Some other antiprotozoal drugs may affect the oxidative status and may increase MDA level and decrease SOD level. In this study, MDA, which is an indicator of lipid peroxidation in vivo, was used to partially detect developing lipid peroxidation. Changes in the levels of reduced GPX and CAT enzymes could be attributed to their use in mediating the hydrogen peroxide detoxification mechanisms. The absence of significant changes in the TAS levels in this study suggests that buparvaquone may partially induce oxidative stress by producing hydrogen peroxide, but no significant changes occurred in the oxidative stress level because of the high antioxidant capacity of sheep. In this study, buparvaquone caused a statistically significant increase (P < 0.05) in the level of Tn-I, which is a marker of specific cardiac damage (P < 0.05), whereas there was no statistically (P > 0.05) significant increase in CK-MBmass. Tn-I and CK-MB levels, which are used to define heart damage in humans, have been successfully used to determine heart damage in sheep. In this research study, the statistically significant increases in Tn-I but not CK-MBmass levels could be considered indicative of mild cardiac damage.Keywords: ram, buparvaquone, safety.


Biology ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 414
Author(s):  
Gal Avishai ◽  
Idan Rabinovich ◽  
Hanna Gilat ◽  
Gavriel Chaushu ◽  
Liat Chaushu

Sialolithiasis is a chronic disease in which a sialolith (salivary stone) causes recurrent inflammation of the affected salivary gland. Anemia of inflammation is a well-described pathology in which a chronic inflammatory disease leads to a reduction in the red blood cell count, hemoglobin and hematocrit values. In this retrospective cohort study, we aim to find whether removal of the sialolith and alleviation of the inflammation affect the complete blood count results. We examined data regarding forty-nine patients who underwent surgery for the removal of a submandibular gland sialolith using the duct-stretching technique. Complete blood counts two years before and after the surgical procedure were collected. The average pre-procedure and post-procedure values were calculated for each patient to establish the average blood profile. The pre- and post-procedure values were compared to evaluate the effect of the surgical treatment on the blood profile. We found that the average blood count values for patients with sialolithiasis were towards the lower end of the normal range. Post-surgery, a significant increase in hematocrit, hemoglobin and red blood cell count was observed, which was more pronounced in the older age group and in patients with co-morbidities. We conclude that sialolith removal surgery is associated with significant improvement in the complete blood count values, especially in the elderly and in patients and with co-morbidities. The speculated pathogenesis is relative anemia of inflammation.


2010 ◽  
Vol 30 (1) ◽  
pp. 25-33 ◽  
Author(s):  
SE Atawodi ◽  
AC Ene ◽  
DA Ameh

The possible hepatotoxic effects of chloroform extract of Artemisia maciverae was evaluated biochemically and histologically using male Swiss albino rats, randomly assigned into four groups of 24 animals each. The groups (control, 50, 100 and 200 mg/kg body weight) were treated for 60 days and then monitored for another 30 days before sacrifice. Alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, bilirubin (total and direct), total protein and albumin were assessed colorimetrically, while tissue specimens were subjected to histological examination following standard hematoxyline-eosin staining techniques. After 1 week of treatment, the extract caused statistically significant elevation in levels of serum alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase and bilirubin (total and direct), while there was significant (p < 0.05) decrease in the levels of serum total protein and albumin at the onset of treatment when compared with the control. These abnormalities in the levels of serum biochemical parameters were spontaneously corrected within 2 weeks of treatment. Similarly, histological assessment showed severe hepatic tissue injuries after 1 week, but these organs recovered spontaneously by the second week of treatment. The results indicate that long-term exposure to therapeutic doses of chloroform extract of A maciverae is relatively safe, but high dose exposure may result in hepatocellular injury.


2015 ◽  
Vol 44 (4) ◽  
pp. 195-199 ◽  
Author(s):  
Priscilla Barbosa Ferreira Soares ◽  
Camilla Christian Gomes Moura ◽  
Huberth Alexandre da Rocha Júnior ◽  
Paula Dechichi ◽  
Darceny Zanetta-Barbosa

<title>Abstract</title><sec><title>Objective</title><p>Evaluate the biological performance of titanium alloys grade IV under different surface treatments: sandblasting and double etching (Experimental surface 1; Exp1, NEODENT); surface with wettability increase (Experimental surface 2; Exp2, NEODENT) on response of preliminary differentiation and cell maturation.</p></sec><sec><title>Material and method</title><p>Immortalized osteoblast cells were plated on Exp1 and Exp2 titanium discs. The polystyrene plate surface without disc was used as control group (C). Cell viability was assessed by measuring mitochondrial activity (MTT) at 4 and 24 h (n = 5), cell attachment was performed using trypan blue exclusion within 4 hours (n = 5), serum total protein and alkaline phosphatase normalization was performed at 4, 7 and 14 days (n = 5). Data were analyzed using one-way ANOVA and Tukey test.</p></sec><sec><title>Result</title><p>The values of cell viability were: 4h: C– 0.32±0.01<sup>A</sup>; Exp1– 0.34±0.08<sup>A</sup>; Exp2– 0.29±0.03<sup>A</sup>. 24h: C– 0.43±0.02<sup>A</sup>; Exp1– 0.39±0.01<sup>A</sup>; Exp2– 0.37±0.03<sup>A</sup>. The cell adhesion counting was: C– 85±10<sup>A</sup>; Exp1- 35±5<sup>B</sup>; Exp2– 20±2<sup>B</sup>. The amounts of serum total protein were 4d: C– 40±2<sup>B</sup>; Exp1– 120±10<sup>A</sup>; Exp2– 130±20<sup>A</sup>. 7d: C– 38±2<sup>B</sup>; Exp1– 75±4<sup>A</sup>; Exp2– 70±6<sup>A</sup>. 14 d: C– 100±3<sup>A</sup>; Exp1– 130±5<sup>A</sup>; Exp2– 137±9<sup>A</sup>. The values of alkaline phosphatase normalization were: 4d: C– 2.0±0.1<sup>C</sup>; Exp1– 5.1±0.8<sup>B</sup>; Exp2– 9.8±2.0<sup>A</sup>. 7d: C– 1.0±0.01<sup>C</sup>; Exp1– 5.3±0.5<sup>A</sup>; Exp2– 3.0±0.3<sup>B</sup>. 14 d: C– 4.1±0.3<sup>A</sup>; Exp1– 4.4±0.8<sup>A</sup>; Exp2– 2.2±0.2<sup>B</sup>. Different letters related to statistical differences.</p></sec><sec><title>Conclusion</title><p>The surfaces tested exhibit different behavior at dosage of alkaline phosphatase normalization showing that the Exp2 is more associated with induction of cell differentiation process and that Exp1 is more related to the mineralization process.</p></sec>


Author(s):  
D. O. Okunlola ◽  
M. D. Shittu ◽  
O. O. Olatunji ◽  
A. J. Amuda ◽  
B. O. Olateju ◽  
...  

The blood profile and histology of selected tissues (kidney, lungs, intestine, and epididymis) of Balami Sheep fed Shea Cake meal supplement was investigated. A total of thirty-two (32) sheep of the average weight range of 11.75-14.75kg comprising an equal number (16) of males and females were randomly allotted to four dietary treatments in a completely randomized experimental design with eight sheep per treatment for twelve weeks duration. Treatment 1 (T1) was a control diet and had zero inclusion level of Shea cake meal (SCM), while treatments 2,3, and 4 had 10%,15%, and 20%  inclusion of SCM, respectively. At the twelfth week of the experiment, two blood samples of 5ml each were taken from each animal from the jugular vein. The first set was preserved in Ethylene Diamine Tetra Acetic Acid (EDTA) bottle for haematological parameters, while the second samples were preserved without EDTA for serum examination. Representative samples of tissues were randomly selected and slaughtered for gross microscopic examination. There was a significant difference (P<0.05) at various levels of inclusion for parameters investigated. The white blood cell (WBC), Red blood cell (RBC), and Packed cell volume (PCV) recorded the highest values at T3 (15% inclusion level) (13.42 x109/L), 2.92x1012/L, 3.75%, respectively). The serum parameters were significantly(p <0.05)different among the treatments. Total protein ranges from 54.78(mg/dl) (T1) - 60.51(mg/dl) (T2), Cholesterol70.15(mg/dl) (T1) - 78.00(mg/dl) (T4). Iron ranges from 58.15(ug/dl) (T1) - 62.88(ug/dl) (T3).The histopathology of tissues (kidney, lungs, intestine, and epididymis) showed no alteration across the treatments (P= .05) in the gross microscopic examinations. No lesion was observed in the lungs, kidney, epididymis, and intestine. The results showed that shea cake meal posed no health risk to Balami sheep and as such recommended as a good supplement in the diets of the sheep.


1982 ◽  
Vol 16 (2) ◽  
pp. 133-137 ◽  
Author(s):  
Elsbeth J. Lee ◽  
W. E. Moore ◽  
H. C. Fryer ◽  
H. C. Minocha

Female, male and castrated male ferrets were studied. Weight gain plateaued at 28-weeks of age with males about 500 g heavier than females. No statistically significant differences in haematology were observed with age, but alkaline phosphatase and alanine aminotransferase levels fell while glucose increased. Haemolysis led to various changes including marked increases in total protein, albumin, inorganic phosphate and sorbitol dehydrogenase.


2021 ◽  
Author(s):  
OLuwatosin Christie Raimi ◽  
Abiodun Adefunmilayo Adeloye

Abstract This study investigated the effects of feeding ensiled graded combinations of cocoa pod, cassava pulp and Acacia leaf on haematological and serum biochemical of West African Dwarf goats. In the bid to control environmental pollution, the wastes of cocoa and cassava were applied as alternative feed ingredients for the goat in spite of its low nutrients’ quality and digestibility. A total of Twenty-eight (28) WAD goats (bucks), aged 4 to 5 months with average body weight of 7.00±0.2kg was used in a completely randomized design for a 45 days study to evaluate the blood profiles of goats fed diet combinations (0:60:40) to (60:0:40). The results revealed significant differences in some of the blood parameters measured among the treatments. The red blood cell, packed cell volume and haemoglobin ranged from 4.90-7.87x106/ul, 19.25-26.83% and 7.33-11.15g/L respectively. The concentrations of mean corpuscular volume, mean corpuscular haemoglobin and mean corpuscular haemoglobin concentration varied significantly (p < 0.05), white blood cell (5.57-9.75x109L) were comparable and lymphocytes (%) ranged from 43.85-55.67. For serum biochemistry, values obtained for total protein and albumin with sodium followed the same trend while cholesterol, urea, creatinine and glucose followed the same trend. Alanine amino transferase, Aspartate aminotransferase and Alkaline phosphatase varied significantly (p<0.05) among the treatment groups. Since the parameters measured were within the normal range for goats’ blood profile, combinations of cocoa pod, cassava pulp with acacia leaves in the ratio of 0:60:40 to 20:40:40 as browse plants do not have any deleterious effect on the health of the animals.


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