scholarly journals Natrum mur 200c promotes seed germination and increases total protein, chlorophyll, rubisco and sugar in early seedlings of cowpea under salt stress

2021 ◽  
Vol 11 (40) ◽  
pp. 128-128
Author(s):  
Sandhimita Mondal ◽  
Nirman Chandra Sukul ◽  
Soma Sukul (nee Chunari)
Keyword(s):  
Salt Stress ◽  
Seed Germination ◽  
Group Treatment ◽  
Total Protein ◽  
Growth And Yield ◽  
Total Protein Content ◽  
Distilled Water ◽  
Significance Level ◽  
Salt Consumption ◽  
Stressed Group

Background: High level of salinity deteriorates seed germination, growth and yield of crops in cultivated lands all over the world. There is no effective remedy to mitigate this global problem. Homeopathy offers a remedy like Natrum mur which at ultra high dilution ameliorates diseases of patients having strong desire for salt consumption. The purpose of the present study is to see whether potentized Natrum mur could alleviate salt stress in germinating seeds of cowpea Vigna unguiculata (L) Walp. Methods: Water-soaked seeds were kept over moist filter paper in covered petridishes which were divided into 5 groups: (1) unstressed and untreated control in sterile distilled water, (2) in 100mM sodium chloride solution, (3) seeds pretreated with Natrum mur 200c and then kept in sterile distilled water, (4) seeds pretreated with Natrum mur 200c and then transferred to 100mM NaCl solution and (5) seeds pretreated with 90% ethanol. Both Natrum mur 200 c and its diluent medium 90% ethanol were diluted with distilled water 1:100 before use for treatment. Results: Natrum mur 200c increased the rate of seed germination, seed water content and growth of seedlings. The drug also enhanced chlorophyll, soluble and insoluble sugar, rubisco and total protein content as compared to the untreated salt stressed group. Treatment with Natrum mur 200c increased salt tolerance in the seedlings as compared to the untreated salt stressed group. All the data were analyzed by ANOVA and the significance level was not less than 1%. Conclusion: Natrum mur 200c reversed the effects of salt stress in germinated seeds thereby providing evidence for Hahnemann’s similia principle in plants. Potentized Natrum mur could be safely used with profit on plants grown on brackish soil.

Alleviation of salt stress using gibberellic acid in Chinese cabbage

2012 ◽  
Vol 60 (4) ◽  
pp. 345-355 ◽  
Author(s):  
M. Jamil ◽  
M. Ashraf ◽  
E. Rha
Keyword(s):  
Salt Stress ◽  
Gibberellic Acid ◽  
Chinese Cabbage ◽  
Germination Rate ◽  
Harmful Effect ◽  
Seed Priming ◽  
Nacl Stress ◽  
Growth And Yield ◽  
Distilled Water ◽  
Membrane Injury

Salinity reduces plant growth and yield by affecting morphological and physiological processes. To alleviate the harmful effects of salt stress various approaches involving plant hormones are used. In this study several parameters involving the measurement of cell membrane injury were used to observe whether stress tolerance could be enhanced in Chinese cabbage (B. oleracea capitata L. Chinensis group) by soaking the seeds for 10 h in distilled water (control), or in 100, 150 or 200 mg l−1 gibberellic acid (GA3). The NaCl concentrations were 0 (control), 50, 100 and 150 mM. Seed treated with GA3 showed increased water uptake and decreased electrolyte leakage as compared to that of distilled water-primed seeds even 24 h after soaking under control conditions. Seed priming with GA3 increased the final germination and the germination rate (1/t50, where t50 is the time to 50% germination) under salt stress conditions. Seed priming also alleviated the harmful effect of salt stress on cabbage in terms of fresh and dry weights. Leaf area was higher in plants raised from seeds primed with the higher GA3 concentrations as compared with those raised from seeds treated with distilled water under control conditions (without NaCl) or at 50 mM NaCl stress. The chlorophyll content increased with the NaCl concentration, especially in plants grown from seeds primed with GA3. Plants grown from GA3-primed seeds also suffered lower cellular injury both under control conditions and under NaCl stress.

scholarly journals STANDARDIZATION OF GERMINATION TEST AND RESPONSE TO NACL SALT STRESS IN Toona cliliata SEEDS

FLORESTA ◽  
2015 ◽  
Vol 45 (4) ◽  
pp. 845 ◽  
Author(s):  
Letícia Ramon De Medeiros ◽  
Manoela Andrade Monteiro ◽  
Patrícia Migliorini ◽  
Marilia Lazarotto ◽  
Lilian De Tunes
Keyword(s):  
Salt Stress ◽  
Sodium Chloride ◽  
Seed Germination ◽  
Dry Matter ◽  
Distilled Water ◽  
Germination Test ◽  
Light Regimes ◽  
Seedling Length ◽  
Toona Ciliata ◽  
Germinated Seeds

AbstractThe objective of this study was to evaluate the germination of Australian cedar seeds (Toona ciliata M. Roem) in three different substrates and two photoperiods and evaluate the effect of salt stress with sodium chloride (NaCl) on the viability and vigor of seeds, conducting two experiments. The first experiment was conducted in a constant temperature room at 25 C and two light regimes 12h 24h white light; and three substrates: sand, between paper and on paper. Evaluations were performed on the seventhand twenty-first day after sowing. Results were expressed as percentage of Normal Seedlings, Abnormal Seedlings and Dead Seeds, Number of True Leaves, Fresh, Dry Matter, Seedling Length and Root. The second experiment used constant 25 C and photoperiod of 12 hours light. With treatments T1 (0mM: distilled water); 25mMNaCl T2; T3 50mMNaCl; T4 and T5 100mMNaCl 75mMNaCl. We evaluated PCG, G, IVG, CP, CR. The photoperiod of 24h light, does not influence the final seed germination and the use of paper on substrate provided the highest percentage of germinated seeds. As to the effect of salt stress, the concentration of 50mMsalt, caused damage to the development of seedlings.ResumoPadronização do teste de germinação e resposta ao estresse salino por NaCl em sementes de Toona ciliata. O objetivo do trabalho foi avaliar a germinação de sementes de cedro australiano (Toona ciliata M. Roem) em três substratos com dois fotoperíodos e avaliar o efeito do estresse salino com cloreto de sódio (NaCl) na viabilidade e vigor de sementes. O experimento I foi conduzido em ambiente com temperatura constante a 25 ºC e dois regimes de luminosidade, 12h de 24h de luz branca; três substratos: areia, entre papel e sobre papel. As avaliações foram realizadas no sétimo e vigésimo primeiro dia após a semeadura. Os resultados expressos em porcentagem de Plântulas Normais, Plântulas Anormais e Sementes Mortas, número de Folhas Verdadeiras, Massa Fresca, Massa Seca, Comprimento de Plântula e Raiz. O experimento II utilizou temperatura constante a 25 ºC e fotoperíodo de 12 horas luz. Com os tratamentos T1 (0 mM: água destilada); T2 25 mMNaCl; T3 50 mMNaCl; T4 75 mMNaCl e T5 100 mMNaCl. Avaliando-se PCG, G, IVG, CP e CR. O fotoperíodo de 24h de luz, não influencia na germinação e o uso do substrato sobre papel proporcionou a maior germinação. Quanto ao efeito do estresse salino, a concentração de 20 mM de sal, prejudicou o desenvolvimento das plântulas.Palavras-chave: Sementes florestais; análise de sementes, vigor, salinidade.

scholarly journals Effects of Sodium Chloride and Sodium Sulfate on Haloxylon ammodendron Seed Germination

2020 ◽  
Vol 12 (12) ◽  
pp. 4927
Author(s):  
Zhazira Zhumabekova ◽  
Xinwen Xu ◽  
Yongdong Wang ◽  
Chunwu Song ◽  
Alzhan Kurmangozhinov ◽  
...  
Keyword(s):  
Salt Stress ◽  
Seed Germination ◽  
High Salinity ◽  
Seed Viability ◽  
Gobi Desert ◽  
Distilled Water ◽  
Haloxylon Ammodendron ◽  
High Recovery ◽  
Desert Areas ◽  
Germinated Seeds

Haloxylon ammodendron is a perennial xerophyte that can survive in extremely harsh desert conditions of Central Asia. This study evaluated the effect of salinity, and their ability to recover on seed germination of H. ammodendron, which were collected at three different desert areas, Bakanas takyr plain (H1), Gurbantüngüt Desert (H2), and Gobi Desert (H3), respectively. Seeds were treated with different concentrations of NaCl and Na2SO4 (0.00 (control), 0.05, 0.10, 0.20, 0.40, 0.60, 0.80, 1.00, 1.20, and 1.40 mol/L) to detemine the germination and recover ability to salt stress. The results of the study were that H. ammodendron were more resistant to Na2SO4 than to NaCl. Regarding inhibition of seed germination H. ammodendron was in the following order: H3 > H2 > H1. Based on the tolerance and recovery, seeds can be demonstrated as follows: NaCl: H3 > H1 > H2; Na2SO4: H1 = H2 = H3. Non-germinated seeds in all salt treatments with low osmotic potential showed high recovery when transferred to distilled water, indicating that these treatments were not too toxic to affect seed viability. These results suggest that H. ammodendron can withstand high levels of salinity at three desert environments. Thus, H. ammodendron could be used to reconstruct vegetation and sustanbility development in the desert areas with high salinity.

scholarly journals Absorption Curve and Germination of Jucá Seeds under Different Salts

2016 ◽  
Vol 8 (7) ◽  
pp. 112
Author(s):  
Danielle Marie Macedo Sousa ◽  
Celsemy Eleutério Maia ◽  
Elis Regina Costa de Morais
Keyword(s):  
Electrical Conductivity ◽  
Salt Stress ◽  
Seed Germination ◽  
Water Absorption ◽  
Electric Conductivity ◽  
Growth And Development ◽  
Saline Solution ◽  
Distilled Water ◽  
Plant Tolerance ◽  
Water Control

<p>The plants are subjected to conditions of multiple stresses that limit their growth and development, which affects their chances of survival wherever they grow. To evaluate the plant tolerance to these conditions, one of the most used methods is the observation of seed germination under salt stress. The action of salts on the seeds varies widely among species and can exert toxic effects, causing damage before and/or after the onset of germination. Therefore, the objective of this work was to evaluate the influence of different salts in water absorption and germination in jucá seeds (<em>Libidibia ferrea</em>). For absorption and germination curve, were used non-scarified and scarified seeds, and subjected to the treatments in distilled water (control) and NaCl solutions, KCl, CaCl<sub>2</sub> and K<sub>2</sub>HPO<sub>4</sub>, in an electric conductivity of 5 dS m<sup>-1</sup>. The saline solution composed by K<sub>2</sub>HPO<sub>4</sub> influence on water absorption of <em>L. ferrea</em> in intact seeds. The scarified and non-scarified seeds of <em>L. ferrea</em> have different times for the physiological phases of water absorption. The action of salts in the germination and vigor in non-scarified and scarified seeds, does not significantly reduce these values due to electrical conductivity of 5 dS m<sup>-1</sup> not be enough to affect the seed germination of <em>L. ferrea</em>.</p>

scholarly journals Assessment of exogenous application of plant growth regulators on Cress seed germination and ?-Galactosidase activity

2020 ◽  
Vol 7 (2) ◽  
pp. 257-263
Author(s):  
Ola O. Alghonmeen ◽  
Khalid Y Alsharafa ◽  
Muhamad O. Al-limoun ◽  
Khaled M. Khleifat ◽  
Ezz Al-Dein Muhammed Al-Ramamneh
Keyword(s):  
Seed Germination ◽  
Plant Growth ◽  
Protein Content ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Total Protein ◽  
Total Protein Content ◽  
Galactosidase Activity ◽  
Negative Effect ◽  
Positive Effect

Plant growth regulators (PGRs) were involved in several types of abiotic stress responses by means of improving seed germination and modifying the growth and development of medicinally important Lepidium sativum via alleviating the negative effects of abiotic stresses. Therefore, the present research was carried out to investigate the effects of exogenous application of PGRson seed germination, protein content and ?-galactosidase activity of L. sativum. Germination of L. sativum seeds was monitored for a short interval after the start of incubation until growth became 100%. While cytokinin treatment showed a positive effect on seed germination more than Gibberellic acid (GA), salicylic acid (SA) produced a higher negative effect than auxins. Quantifying changes in total protein content during seed germination as influenced by PGRs revealed that all PGRs have to exert a positive effect arranged in the following order: SA ? auxin ? cytokinin ? GA. Parallel to changes in germination percentage and total protein content of seed, a negative effect was attainedon ?-galactosidase specific activity in response to PGRs with the following arrangement: SA ? auxin ? cytokinin ? GA.In conclusion, the present study proposed the potential importance of the type and magnitude of exogenously applied PGRs during the germination of easily or even more difficult-to-germinate seeds.

scholarly journals Intoxication of wheat seedlings with 50 mM NaCl and follow-up attempt to “cure” by extremely diluted NaCl (30cH)

2021 ◽  
Vol 14 (2) ◽  
pp. 04-06
Author(s):  
P.C. Endler ◽  
Pia Lamest ◽  
Elke Liebig
Keyword(s):  
Triticum Aestivum ◽  
Salt Stress ◽  
Seed Germination ◽  
Growth And Yield ◽  
Control Groups ◽  
Wheat Seedlings ◽  
The World ◽  
Wheat Germination ◽  
Effective Remedy

Background: “High level of salinity deteriorates seed germination, growth and yield of crops in cultivated lands all over the world. There is no effective remedy to mitigate this global problem”, Mondal, Sukul et al.1 summed up the background of their study that led to the conclusion that homeopathically prepared, extremely diluted Natrum muriaticum (NaCl) 200cH promotes seed germination in seedlings of cowpea under salt stress.1 Aim: To investigate if the wheat germination model standardized at the Interuniversity College2,3 can be useful in investigating the NaCl issue; i.e. the study was not designed as a replication of the study by Mondal, Sukul et al.1 Methods: Grains of winter wheat (Triticum aestivum) were observed under the influence of extremely diluted NaCl (10-60) prepared by stepwise dilution and agitation according to a protocol derived from homeopathy (“N30c”). Analogously prepared water was used for control (“W30c”). Grains (500 per group) were pretreated (intoxicated) with (non-agitated) NaCl 50mM (“N”) or with water (“W”) prior to treatment with N30c or W30c. Seedlings were allowed to develop under standardized conditions for 32 h; germination of stalks and of roots were monitored at intervals of 4 h. Results: With regard to intoxication, the groups treated with N exhibited less growth than the W control groups. With regard to the attempt to “cure”, the N+N30c group was not statistically different from the N+W30c group, and the W+N30c group was not statistically different from the W+W30c group. Conclusion: The hypothesis that treatment of wheat seedlings with the extreme dilution NaCl 30cH will “cure” the effect of previous intoxication with NaCl 50 mM on seed germination could not be accepted. If further experiments are to be performed on the wheat model, these should include different intoxication solutions (e.g. 100mM1), different treatment dilutions (e.g. 200cH1) or different durations of the experiment3. The use of cowpea1 instead of wheat should also be considered.

Seed-borne fungi of cowpea [Vigna unguiculata (L.) Walp] and their possible control in vitro using locally available fungicides in Botswana.

2016 ◽  
Vol 5 (11) ◽  
pp. 5016 ◽  
Author(s):  
K. B. Khare* ◽  
Loeto D. ◽  
Wale K. ◽  
Salani M.
Keyword(s):  
Seed Germination ◽  
Continuous Light ◽  
Sodium Hypochlorite Solution ◽  
Distilled Water ◽  
Percentage Germination ◽  
Department Of Agriculture ◽  
Hypochlorite Solution ◽  
Cowpea Cultivars ◽  
Petri Plate

Seeds of three cowpea cultivars namely Black eye, ER 7 and Tswana obtained from the Department of Agriculture Research, Gaborone were tested for the presence of seed-borne fungi, and their possible control in vitro using locally available fungicides. Four hundred fifty seeds of each cultivar of cowpea were disinfected with 2% sodium hypochlorite solution for 10 min and washed three times with sterile distilled water before placing them in PDA plates (5 seeds/9 cm Petri plate), incubated at 22±2o C for 12 hour each under continuous light and dark. A total of eight fungi were detected from seeds of cowpea. These were Aspergillus flavus, A. niger, Cylindrocarpon sp., Fusarium equisiti, F. oxyaporum, Penicillium chyrosogenum, Rhizopus oligosporus and R. stolonifer. Rhizopus spp. were dominant fungi recovered from seeds, followed by Penicillium, Aspergillus, Fusarium and Cylindrocarpon. The fungi detected resulted in decay and rotting of seeds, and thereby reducing percentage germination of seeds (22%, 37% and 63 % seed germination in Black eye, ER7 and Tswana varieties of cowpea respectively). Out of four fungicides tested, benlate, captan, dithane M 45 and chlorothalanil. Dithane M45 effectively controlled seed-borne fungi, and enhanced seed germination to an average of 86% (93% germination with no fungi detected in Tswana variety) as compared to chlorothalonile (79%), benlate and captan (77%) and un-treated seeds (45%). The fungal incidence was reduced to 2.3%, 4.3%, 5.3% and 5.3% when seeds were treated with dithane M-45, chlorothalonil, benlate and captan respectively as compared to 62% in non-treated seeds.

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