Pharmacognostical and Phytochemical Evaluation of Aconitum heterophyllum Root

2021 ◽  
pp. 3860-3862
Author(s):  
Anna Mathew ◽  
Chandrashekar. K. S ◽  
Anoop Kishore ◽  
Vasudev Pai ◽  
Aswatha Ram H. N
Keyword(s):  
Moisture Content ◽  
Chemical Constituents ◽  
Phytochemical Screening ◽  
Secondary Phloem ◽  
Starch Grains ◽  
Anatomical Studies ◽  
Tuberous Roots ◽  
Aconitum Heterophyllum ◽  
Powder Analysis ◽  
Wide Zone

The present work aims to study the pharmacognostical and phytochemical characteristics of the roots of Aconitum heterophyllum. The study deals with the macroscopy, microscopy, powder analysis, physicochemical standardization, and preliminary phytochemical screening of Aconitum heterophyllum roots. Anatomical studies of the tuberous roots of Aconitum heterophyllum showed distinct endodermis, a wide zone of secondary phloem, and a ring of 4 to 9 vascular strands. The powder analysis revealed the presence of suberized cells of metaderm, reticulate xylem vessels, and starch grains. Physicochemical standardization such as moisture content, ash values, and extractive values of Aconitum heterophylluym was determined. For the identification of various chemical constituents, preliminary phytochemical screening was also studied.

scholarly journals KARAKTERISTIK DAN SKRINING FITOKIMIA EKSTRAK ETANOL 70% BATANG KEPUH (Sterculia foetida L.)

Jurnal Kimia ◽  
2019 ◽  
Vol 13 (1) ◽  
pp. 22
Author(s):  
N. P. S. E. Cahyani ◽  
J. Susiarni ◽  
K. C.S. Dewi ◽  
N. L.P. Melyandari ◽  
K. W.A. Putra ◽  
...  
Keyword(s):  
Moisture Content ◽  
Water Content ◽  
Ethanol Extract ◽  
Phytochemical Screening ◽  
Residual Solvent ◽  
Solvent Content ◽  
Residual Content ◽  
Ethanol Level ◽  
Anti Inflammatory

Kepuh (Sterculia foetida L) is a type of kapok plant that has been scientifically proven to have activity as an anti-inflammatory and analgesic. 70% ethanol extract of stem stem is obtained by maceration using 70% ethanol. Examination of the characteristics of 70% ethanol extract of kepuh stem included organoleptic, of moisture content and determination of residual solvent content. Phytochemical screening of 70% ethanol extract of kepuh bark includes: alkaloid, flavonoid, saponin, tannin , polyphenol, and examination of glycosides. The results of the examination of the characteristics of 70% ethanol extract of kepuh stem obtained water content of 8.66 ± 0.748%, the residual content of the solvent had a 0 (zero) ethanol level. The results of phytochemical screening showed 70% ethanol extract of stem stem containing steroid compounds, triterpenoids, flavonoids, saponins, tannins and polyphenols. The identification using UV-Vis spectrophotometry produced ? 212, the absorbance was 1.8601 and ? 284, the absorbance was 0.42186.

PHYTOCHEMICAL SCREENING AND GC-MS ANALYSIS OF THE ESSENTIAL OIL EXTRACT OF THE AROMATIC PLANT CYMBOPOGON CITRATUS

2021 ◽  
pp. 28-29
Author(s):  
Umadevi. D ◽  
K.U.M.A Rafeeq
Keyword(s):  
Mass Spectrometry ◽  
Essential Oil ◽  
Chemical Constituents ◽  
Folk Medicine ◽  
Gas Chromatography Mass Spectrometry ◽  
Aqueous Extracts ◽  
Phytochemical Screening ◽  
Aromatic Plant ◽  
Cymbopogon Citratus ◽  
Oil Extract

Cymbopogon citratus, widely known as lemongrass is used as folk medicine for several disease conditions. Preliminary phytochemical screening of the essential oil extract of C. citratus revealed the presence of various bioactive compounds like alkaloids, avonoids, saponins, tannins and phenolic compounds in the aqueous extracts. Essential oil was extracted from C. citratus leaves and its chemical constituents were identied by Gas Chromatography-Mass Spectrometry (GC-MS).

Skrining Fitokimia Enam Genotipe Teh

2014 ◽  
Vol 1 (2) ◽  
pp. 63
Author(s):  
Budi Martono ◽  
Rudi T. Setiyono
Keyword(s):  
Camellia Sinensis ◽  
Chemical Constituents ◽  
The Other ◽  
Preliminary Evaluation ◽  
Phytochemical Screening ◽  
Tea Leaves ◽  
Randomized Design ◽  
Medicinal Crops ◽  
Completely Randomized Design

<p>Skrining fitokimia dimaksudkan untuk melakukan evaluasi pendahuluan tentang kandungan kimia pada teh (Camellia sinensis). Selain itu, teh mengandung katekin yang dapat digunakan sebagai petunjuk kualitas dari daun teh. Penelitian bertujuan mengetahui kandungan senyawa aktif dan kadar katekin pada teh. Penelitian dilaksanakan mulai bulan April sampai dengan Juni 2012 di laboratorium Pengujian Balai Penelitian Tanaman Rempah dan Obat, Bogor. Skrining fitokimia pucuk peko dengan dua daun (p+2) dilakukan berdasarkan prosedur dari Materia Medika Indonesia (MMI), sedangkan analisis katekin dengan menggunakan metode SNI gambir. Penelitian disusun berdasarkan rancangan acak lengkap (RAL), enam perlakuan dengan empat ulangan. Perlakuan yang digunakan adalah enam genotipe teh (Tbs 1, Tbs 2, Hibrid, Cin 143, Rb 3, dan Kiara 8). Hasil penelitian menunjukkan keenam genotipe yang diuji mengandung senyawa alkaloid, saponin, tanin, fenolik, flavanoid, steroid, dan glikosida. Genotipe Tbs 1, Hibrid, dan Kiara 8 positif mengandung senyawa triterpenoid, sedangkan Tbs 2, Rb 3, dan Cin 143 negatif. Genotipe Tbs 1 dan Tbs 2 memiliki kandungan katekin paling tinggi (kecuali bagian ruas+tangkai daun) dibandingkan dengan empat genotipe lainnya. Pucuk peko, daun pertama, dan daun kedua pada genotipe Tbs 1 memiliki kadar katekin masing-masing 17,92%, 11,73%, dan 14,67%, sedangkan pada genotipe Tbs 2 masing-masing 18,22%, 13,48%, dan 15,81%. Kadar katekin terendah dihasilkan oleh bagian ruas+tangkai daun pada genotipe Rb 3 (1,78%). Pucuk peko menghasilkan kandungan katekin bervariasi antara 8,36%-18,22%, lebih tinggi dibandingkan dengan daun pertama, daun kedua, dan bagian ruas + tangkai daun.</p><p>Kata kunci: Camellia sinensis, fitokimia, genotipe, katekin, pucuk peko</p><p>Phytochemical screening was intended for a preliminary evaluation of the chemical constituents of the tea (Camellia sinensis). In addition, tea also contains catechin that can be used as an indication of the quality of tea leaves. The objectives of this study were to determine the content of the active compounds and catechin in tea. The research was conducted from April to June 2012 in the Laboratory of the Research Institute for Spices and Medicinal Crops, Bogor. The phytochemical screening was performed based on the procedure of Materia Medika Indonesia (MMI), while the catechin analysis used the method of SNI gambir. The study was carried out in completely randomized design with six treatments and four replications. The treatments used are six tea genotypes namely Tbs 1, Tbs 2, Hibrid, Cin 143, Rb 3, and Kiara 8. The results showed that the six tea genotypes tested contained the compounds of alkaloid, saponin, tannin, phenolic, flavanoid, steroid, and glycoside. Positively triterpenoid compounds present in the genotype of Tbs1, Hybrids, and Kiara 8, and negative in Tbs 2, Rb 3, and Cin 143. The genotypes of Tbs 1 and Tbs 2 produced the highest catechin content compared to the other genotypes. Catechin content was lowest in the part of internodes+leaf stalk of Rb 3 (1.78%). Pecco shoots produce catechin content of about 8.36%-18.22%, higher than the first leaf, second leaf, and the parts of internodes+leaf stalk.</p>

Phytochemical and Nutritional Values of Local Plants in the Phraek Nam Daeng Community Samut Songkhram Province Thailand

2018 ◽  
Vol 879 ◽  
pp. 101-107
Author(s):  
Udomdeja Polyium ◽  
Na Thaisong Phinthida
Keyword(s):  
Moisture Content ◽  
Protein Content ◽  
Cardiac Glycoside ◽  
Carbohydrate Content ◽  
Fiber Content ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Sesuvium Portulacastrum ◽  
Acanthus Ilicifolius ◽  
Local Plants

The biodiversity of local plants on Phraek Nam Daeng community Amphawa, Samut Songkhram province, Thailand has been the result of the terrain as a source of freshwater and salty. This study was carried out to evaluate the phytochemical screening and nutritional value of 6 local plants in the Phraek Nam Daeng community including: Azima sarmentosa Benth, Pluchea indica (L) Less,, Boehravia diffusa L, Acanthus ilicifolius L, Sesuvium portulacastrum L and Derris trifoliata Lour. The phytochemical analysis was performed for alkaloid, tannin, terpenoid, flavonoid, anthraquinone and cardiac glycoside. Nutritional analysis of proximal composition of local plants using the method of AOAC (2005),Which consist of moisture content, protein content, lipid content, fiber content, total ash content and carbohydrate content. The results showed that phytochemical screening of 6 local plants, the methanolic extracts have the presence of bioactive substances such as alkaloid, tannin, terpenoid, flavonoid, anthraquinone and cardiac glycoside. The most nutritional composition of local plants showed that the moisture content was Sesuvium portulacastrum L, protein content was Acanthus ilicifolius L, lipid and carbohydrate content was Azima sarmentosa Benth. & Hook, fiber content was Pluchea indica (L) Less., these represent the potential of food crops for food security and sustainable use of biodiversity.

Phytochemical Analysis and GC-MS Profiling in the Flower of Plumeria alba

2020 ◽  
Vol 981 ◽  
pp. 280-284
Author(s):  
Siti Aisyah Mohamad ◽  
Noor Suhana Adzahar ◽  
Muhammad Nadeem Akhtar ◽  
Seema Zareen ◽  
Thong Chuan Lee
Keyword(s):  
Chemical Constituents ◽  
Volatile Oil ◽  
Gas Chromatography Mass Spectrometry ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Therapeutic Properties ◽  
Phytochemical Constituents ◽  
Screening Assays ◽  
Major Chemical ◽  
Ethylhexyl Phthalate

Therapeutic properties of the medicinal plant are due to the presence of phytochemical constituents. The phytochemical constituents of Plumeria alba flower were investigated by phytochemical screening assays and gas chromatography-mass spectrometry (GC-MS). The phytochemical screening of hexane, dichloromethane, ethyl acetate, butanol, and aqueous extracts of P. alba flower showed it contains a various concentration of saponins, flavonoids, tannins, steroids, volatile oil and phenolic compounds. Several major chemical constituents that were identified is squalene, bis(2-ethylhexyl) phthalate, methyl (methyl 4-O-methyl-α-d-mannopyranoside) uronate and tricyclo [7.2.0.0(2,6)] undecan-5-ol, 2,6,10,10-tetramethyl- (isomer 2) by using GC-MS technique.

Preservation of the Moringa oleifera Constituents by Freeze-drying

2019 ◽  
pp. 1-6
Author(s):  
Semirames Do N. Silva ◽  
Francisco A. C. Almeida ◽  
Josivanda P. Gomes ◽  
Newton C. Santos ◽  
Damião J. Gomes ◽  
...  
Keyword(s):  
Moisture Content ◽  
Freeze Drying ◽  
Moringa Oleifera ◽  
Chemical Constituents ◽  
Physical Parameters ◽  
Edible Plant ◽  
Agricultural Engineering ◽  
Physico Chemical ◽  
Before And After ◽  
Processing And Storage

Aims: Moringa oleifera is an edible plant. A wide variety of nutritional and medicinal virtues have been attributed to its roots, barks, leaves, flowers, fruits and seeds. The objective of this research was to evaluate the preservation of the constituents of the powder obtained from the moringa seeds by freeze-drying comparing it with the in natura (natural extract). Place and Duration of Study: The work was conducted at the Laboratory of Processing and Storage of Agricultural Products, Department of Agricultural Engineering, Federal University of Campina Grande, Brazil, in the period from August to November 2018. Methodology: The seeds were peeled and macerated manually. Freeze-drying was done in a Liotop® L101 benchtop freeze drier. After dehydration the samples were disintegrated and the physical and physico-chemical constituents were evaluated before and after freeze-drying in terms of apparent density, real density, porosity, compacted density, compressibility index, Hausner factor, solubility, moisture content and activity, ash, titratable total acidity, pH, protein, lipids and carbohydrates. Results: The in natura powder presented better results for the physical analyzes of the densities: apparent, real and compacted, however, it was observed that for the other physical parameters and physicochemical constituents the freeze-drying promoted the preservation of these in front of the in natura. Freeze-drying caused a significant reduction in moisture content, pH and lipid activity, making the powders more stable and contributing to the maintenance of their physico-chemical qualities. The inverse was observed for the ash, protein and carbohydrate contents, where freeze-drying promoted increases in their contents. Conclusion: Freeze-drying presents as an appropriate method in the preservation of moringa constituents, with emphasis on physicochemical.

scholarly journals EVALUASI DAN IDENTIFIKASI GOLONGAN SENYAWA POTENSIAL ANTIBAKTERI PADA DAUN DAN KULIT BATANG MIMBA (Azhadirachta indica A. Juss) TERHADAP Escherichia coli

2019 ◽  
Vol 11 (2) ◽  
pp. 40-48
Author(s):  
Arif Setiawansyah ◽  
Aliefman Hakim ◽  
Dyke Gita Wirasisya
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Carboxylic Acid ◽  
Azadirachta Indica ◽  
Crude Extract ◽  
Chemical Constituents ◽  
Hexane Fraction ◽  
Diffusion Method ◽  
Phytochemical Screening ◽  
Neem Leaves

ABSTRACT Neem (Azadirachta indica A.Juss) is a plant that potentially developed for antibacterial agent for both the leaves and barks. The aims of this study were to compare the effectiveness of the antibacterial activity of neem leav es and stem barks extract and to identify the antibacterial compounds of the most active fractions. The extraction method was done using sonication method. Antibacterial activity was evaluated using wells solid diffusion method and TLC-Bioautography. Extract fractionation was conducted using liquid-liquid partitioning method. The chemical compounds of extracts and fractions were analyzed using TLC and GCMS. The result of sonication extraction obtained neem leaves oil (12,02%), leaves crude extract (4,3%) and stem barks crude extract (16,85%). The major chemical constituents of GCMS analysis are 2,3-Dihydro-3,5-dihydroxy-6-methyl-4H-pyran-4-one (6,06%), L-proline,1-Acetyl-(CAS) Acetylproline (5,85%), 4-hydroxy-2-methyl-pyrrolidine-2-carboxylic acid (21,42%), 2,3-Dyhidrobenzofuran (2,69%), alpha-D-methylglucopyranoside (4,54%), palmitic acid (2,92%), Arabino-hex-1-enitol, 1,5-Anhydro-2-deoxy-(CAS) glucal (31,69%). Phytochemical screening of neem leaves oil, leaves and barks crude extract revealed the presence of alkaloids, flavonoids, phenols, saponins, triterpenoids, steroids and sterols. Antibacterial test results showed neem leaves oil was more effective than leaves and stem barks crude extract against Escherichia coli. The n-hexane fraction showed higher antibacterial activity than ethyl acetate fraction and ethanol fraction. Phytochemical screening of n-hexane fraction showed the presence of triterpenoids, steroids, sterols and phenols.   Mimba (Azadirachta indica A.Juss) merupakan tanaman yang berpotensi dikembangkan sebagai antibakteri baik bagian daun maupun kulit batang. Penelitian ini bertujuan untuk membandingkan efektivitas antibakteri ekstrak daun dan kulit batang mimba terhadap Escherichia coli dan untuk mengidentifikasi golongan senyawa potensial antibakteri pada fraksi teraktif. Pengujian aktivitas antibakteri dilakukan dengan metode difusi padat menggunakan sumuran dan KLT-Bioautografi. Fraksinasi ekstrak dilakukan dengan metode partisi. Komponen kimia ekstrak dan fraksi dianalisis menggunakan KLT dan GCMS. Hasil ekstraksi sonikasi diperoleh minyak daun (12,02%), ekstrak kasar daun (4,3%) dan ekstrak kasar kulit batang (16,85%). Skrining fitokimia menunjukkan minyak daun, ekstrak kasar daun dan ekstrak kulit batang mimba mengandung senyawa alkaloid, flavonoid, fenolik, saponin, triterpenoid, steroid dan sterol. Komponen kimia mayor hasil analisis GCMS minyak daun mimba adalah 2,3-dihydro-3,5-dihydroxy-6-methyl-4H-pyran-4-one (6,06%), L-proline,1-Acetyl-(CAS)Acetylproline (5,85%), 4-hydroxy-2-methyl-pyrrolidine-2-carboxylic acid (21,42%), 2,3-dyhidrobenzofuran (2,69%), Alpha-d-methylglucopyranoside (4,54%), Asam Palmitat (2,92%), Arabino-hex-1-enitol, 1,5-anhydro-2-deoxy-(CAS)glucal (31,69%). Hasil uji antibakteri menunjukkan minyak daun lebih efektif menghambat pertumbuhan Escherichia coli dibandingkan dengan ekstrak kasar daun dan kulit batang. Fraksi n-heksan menunjukkan aktivitas antibakteri paling besar dibandingkan dengan fraksi etil asetat dan etanol. Hasil skrining fitokimia fraksi n-heksan menunjukkan adanya senyawa triterpenoid, steroid, sterol dan fenolik.

scholarly journals LC-ESI/MS and GC-MS Methanol Extract Analysis, Phytochemical and Antimicrobial Activity Studies of Centella asiatica

2020 ◽  
pp. 32-51
Author(s):  
Daniel A. Ondeko ◽  
Bernard F. Juma ◽  
Lilechi D. Baraza ◽  
Peter K. Nyongesa
Keyword(s):  
Chemical Constituents ◽  
Centella Asiatica ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Phytochemical Screening ◽  
Disc Diffusion Method ◽  
Leaf Extracts ◽  
Methanolic Extracts ◽  
Antibacterial And Antifungal

Aims: To determine chemical constituents of the Leaf extracts of Centella asiatica using the LC-MS and GC-MS and their antimicrobial activities. Study Design: Structural determination of compounds from the leaf extracts was done using GC-MS and LC-MS analysis. The antimicrobial properties of the extracts were done using disc diffusion method. Place and Duration of Study:  Pure and Applied Chemistry Department, Masinde Muliro University of Science and Technology, Kenya: Between 2016-2019. Methodology: Plant materials of C. asiatica were sequentially extracted separately based on the polarity viz., hexane, ethyl acetate and methanol. Determination of chemical constituents was done using LC-MS and GC-MS analysis and phytochemical screening. The extracts were assayed against Staphylococcus aureus, Klebsiella pneumonia, Escherichia coli and Candida albicans. Standard antimicrobials viz. ampicillin and Nystatin were used as the control. Disc diffusion method was used and zones of inhibition, after respective incubation periods, were used to quantify antibacterial and antifungal activity. Results: Phytochemical screening of the hexane, ethyl acetate and methanolic extracts showed that terpenoids, flavonoids, saponins, alkaloids, steroids, amino acids and proteins, reducing sugars and carbohydrates were present. LC-MS and GC-MS analyses of the methanolic extracts identified 22 and 33 compounds, respectively, by use of the national institute of standards and technology (NIST) library. The extracts showed appreciable activity against common microbes tested. Conclusion: This study forms the basis for the biological characterization and significance of the compounds identified in the leaf and stem extracts of C. asiatica. These compounds are known to possess antibacterial and antifungal activities that could be established as potential candidates for future drug development. However, these extracts, need to be subjected to further chromatographic procedures to isolate the identified compounds and their bioactivities determined.

scholarly journals Phytochemical Screening of Flowers from Moringa oleifera Lam.

2018 ◽  
Vol 1 (01) ◽  
pp. 31-35
Author(s):  
V S Suryawanshi ◽  
S R Umate
Keyword(s):  
Moringa Oleifera ◽  
Unsaturated Fatty Acids ◽  
Chemical Constituents ◽  
Ethanol Extract ◽  
Pharmacological Properties ◽  
Phytochemical Screening ◽  
Flower Extract ◽  
Plant Sources ◽  
Potassium Magnesium ◽  
Moringa Oleifera Lam

Plants serve as a vast source for varied phytoconstituents exhibiting varied pharmacological properties. It is one of the richest plant sources of Vitamins A, B (Ramachandran, 1980; Odee, 1998; Jahn et al, 1986; Palada et al, 1995; Gilani et al, 1994) Vit. C, D, E and K. The vital minerals present in Moringa include Calcium, Copper, Iron, Potassium, Magnesium, Manganese and Zinc. Leaves, flowers, and pods are common vegetables in the Asian diet. The chemical constituents of the Hexane and Ethanol extract of Moringa oleifera Lam. Flowers were investigated. Different chemical constituents were identified in the extract; unsaturated fatty acids, especially oleic acid, alkaloids, steroids, flavonoids, tannins, saponins, carbohydrates, and minerals are present in the extract. This work aims to identify the main constituents from Moringa oleifera Lam. Flower extract.

scholarly journals Phytochemical Screening And Tyrosinase Inhibition Activity Of Leaves Cassia siamea L.

2020 ◽  
Vol 12 (2) ◽  
pp. 198
Author(s):  
Munawarohthus Sholikha ◽  
Ainun Wulandari
Keyword(s):  
Methanol Extract ◽  
Chemical Constituents ◽  
Tropical Region ◽  
Chloroform Fraction ◽  
Phytochemical Screening ◽  
Tyrosinase Inhibition ◽  
Cassia Siamea ◽  
Percent Inhibition ◽  
Butanol Fraction ◽  
Chemical Content

Cassia siamea L. has been used traditionally as medicine and in previous studies had an IC­50 antioxidant activity of 144.12 µg / mL. Cassia siamea L. potential as a medicinal substance is thought to have chemical constituents such as saponins, anthraquinones, alkaloids, flavonoids, tannins, terpenoids and steroids. Indonesia, which is located in a tropical region with high temperatures and ultraviolet (UV) radiation, can cause skin disorders such as hyperpigmentation due to excess melanin synthesis. Tyrosinase enzymes can prevent or inhibit melanin formation. The purpose of this study was to determine the chemical content of Cassia siamea L. leaves extract with various solvents and the inhibitory activity of tyrosinase enzymes. In this study, the leaves of Cassia siamea L. were macerated with methanol as a solvent. The dry methanol extract was fractionated using the liquid-liquid method using aquadest, butanol and chloroform as solvents. The four dried extracts were then phytochemical screening to determine their chemical content. After that, the tyrosinase inhibition test was carried out in vitro with L-Dopa as a substrate using ELISA plate well reader at concentrations of 100, 1000 and 100000 ppm with three repetitions (triplo). The methanol extract had percent inhibition of 19.993 ± 1.125%, 28.984 ± 0.624%, 57.164 ± 0.623%, respectively; butanol fraction 25.914 ± 0.541%, 32.566 ± 0.767%, 52.120 ± 1.616%; chloroform fraction 19.920 ± 0.730%, 26.425 ± 0.937%, 62.865 ± 0.167% and aquadest fraction 23.830 ± 0.879%, 30.885 ± 0.778%, 67.471 ± 0.352%. Kojic acid had tyrosinase activity and acts more active than the other extract and fraction with 91.155±0.228% at 500 ppm. The butanol fraction had the highest percent inhibition at 100 and 1000 ppm which was thought to be due to the presence of alkaloids, tannins, flavonoids, steroids and terpenoids from the results of the phytochemical screening test.

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