Isolation and Evaluation of Anti-inflammatory activity of Epigallocatechin from Senegalia rugata along with PUFAs

2021 ◽  
pp. 5739-5744
Author(s):  
Mounica Ponugoti ◽  
Siva Prasad Panda ◽  
Umasankar Kulandaivelu ◽  
GSN Koteswara Rao ◽  
Rajasekhar Reddy Alavala ◽  
...  
Keyword(s):  
Methyl Esters ◽  
Petroleum Ether Extract ◽  
Ethanol Extract ◽  
Spectral Studies ◽  
Dependent Manner ◽  
Stabilization Method ◽  
Membrane Stabilization ◽  
Anti Inflammatory ◽  
Dose Dependent

Senegalia rugata (Lam.) Britton & Rose, Synonym: Acacia concinna (Wild.) DC., Family: Fabaceae is one of the ayurvedic medicinal plant and commonly known as shikakai. The pods of S. rugata are normally used for cleansing of hair naturally due to the presence of higher content of saponins. In this study, we have isolated six compounds consisting of epigallocatechin (monomeric proanthocyanidin) from ethanol extract of S. rugata and a mixture of methyl esters of five polyunsaturated fatty acids (PUFA): methyl oleate, glyceryl trilinoleate, methyl linoleate methyl eicosenoate and methyl vernolate from petroleum ether extract of S rugata. The structures of the six compounds were elucidated using 1HNMR, 13CNMR and IR spectral studies. Epigallocatechin has shown significant in vitro anti-inflammatory property in a dose-dependent manner using the HRBC membrane stabilization method.

Identifikasi Antiinflamasi Partisi Metanol, n-Heksan dan Ekstrak Etanol Daun Kemangi (Ocimum Americanum L.) Secara In Vitro

2021 ◽  
Vol 1 ◽  
pp. 820-828
Author(s):  
Annas Pamening ◽  
W Wirasti ◽  
S Slamet ◽  
Urmatul Waznah
Keyword(s):  
Diclofenac Sodium ◽  
Ethanol Extract ◽  
Positive Control ◽  
Hypotonic Solution ◽  
Stabilization Method ◽  
Membrane Stabilization ◽  
Anti Inflammatory ◽  
Red Blood Cell Membranes ◽  
Ocimum Americanum

AbstractBasil plant (Ocimum americanum) is efficacious as an anti-inflammatory and analgesic activity. According to research by Sarma and Babu, 2011, Verma and Kothiyal, 2012 showed basil activity as an antioxidant, antimicrobial, anti-diabetic, anthelmintic, antifungal, insecticide, anti-inflammatory, analgesic, and lowering total cholesterol and LDL-C levels. The purpose of this study was to determine the stabilization activity of red blood cell membranes on methanol partitioning, n-hexane partitioning and ethanol extract of basil leaves in vitro. This study used the erythrocyte membrane stabilization method from the induction of a hypotonic solution with samples of methanol partitioning, n-hexane partitioning and ethanol extract to be compared with a positive control, namely Na diclofenac. By analyzing the data using UV-Vis spectrophotometry test. These results were supported by the ANOVA statistical test which stated that there was a difference in each treatment and continued with the Tukey test which stated that there was no difference between 100 ppm diclofenac sodium and 400 ppm ethanol extract.Keywords: Extract, Basil (Ocimum americanum) Leaf, In Vitro. AbstrakTumbuhan Kemangi (Ocimum americanum) berkhasiat sebagai aktivitas sebagai anti-inflamasi dan analgesik. Menurut penelitian Sarma dan Babu, 2011.,Verma dan Kothiyal, 2012 menunjukkan aktivitas kemangi sebagai antioksidan, antimikroba, anti diabetes, antihelmintik, antifungi, insektisida, antiinflamasi, analgesic, dan menurunkan kadar total kolesterol dan LDL-C. Tujuan dari penelitian ini yaitu untuk mengetahui aktivitas stabilisasi membran sel darah merah pada partisi metanol, partisi n-heksan dan ekstrak etanol daun kemangi secara in vitro. Penelitian ini menggunakan metode stabilisasi membran eritrosit dari induksi larutan hipotonik dengan sampel partisi metanol, partisi n-heksan dan ekstrak etanol yang akan dibandingkan dengan kontrol positif yaitu Na diklofenak. Dengan analisis data menggunakan uji spektrofotometri UV-Vis. Hasil ini didukung dengan uji statistik ANOVA yang menyatakan terdapat perbedaan pada setiap perlakuan dan dilanjutkan uji tukey yang menyatakan tidak ada perbedaan pada natrium diklofenak 100 ppm dengan ekstrak etanol konsentrasi 400 ppm.Kata Kunci : Ekstrak, Daun Kemangi (Ocimum americanum), In Vitro.

IN VITRO ANTI-INFLAMMATORY AND ANTI-ARTHRITIC ACTIVITY OF ETHANOLIC EXTRACT OF LEAVES OF PYRENACANTHA VOLUBILIS (EEPV)

2020 ◽  
pp. 156-158
Author(s):  
ANJALI P ◽  
VIMALAVATHINI R ◽  
KAVIMANI S
Keyword(s):  
Protein Denaturation ◽  
Diclofenac Sodium ◽  
Ethanolic Extract ◽  
Dependent Manner ◽  
Membrane Stabilization ◽  
Standard Drug ◽  
Human Red Blood Cells ◽  
Anti Inflammatory ◽  
Dose Dependent

Objectives: The study was undertaken to evaluate the in vitro anti-inflammatory and anti-arthritic activity of the ethanolic extract of leaves of Pyrenacantha volubilis (EEPV) using human red blood cells (HRBCs) membrane stabilization and protein denaturation methods. Methods: In the present study, the in vitro anti-inflammatory and anti-arthritic activity of EEPV was carried out using HRBC membrane stabilization by hypotonicity-induced hemolysis and protein denaturation using egg albumin methods at various concentrations (100, 200, 400, 800, and 1000) of EEPV. Diclofenac sodium was used as reference standard. Results: P. volubilis was effective in inhibiting HRBC membrane stabilization and protein denaturation in a dose-dependent manner and was comparable to the standard drug diclofenac sodium. Conclusion: The study suggests that P. volubilis has potential anti-inflammatory and anti-arthritic activity.

scholarly journals Modulation of the Neuroprotective and Anti-inflammatory Activities of the Flavonol Fisetin by the Transition Metals Iron and Copper

Antioxidants ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 1113
Author(s):  
Pamela Maher
Keyword(s):  
Transition Metals ◽  
Neurodegenerative Diseases ◽  
Dependent Manner ◽  
Copper Binding ◽  
Drug Candidates ◽  
Beneficial Effects ◽  
Anti Inflammatory ◽  
Dose Dependent

Alterations occur in the homeostasis of the transition metals iron (Fe2+) and copper (Cu2+) during aging and these are further amplified in neurodegenerative diseases, including Alzheimer’s disease (AD). These observations suggest that the most effective drug candidates for AD might be those that can reduce these alterations. The flavonoid fisetin has both neuroprotective and anti-inflammatory activity both in vitro and in vivo and can bind both iron and copper suggesting that its chelating activity might play a role in its beneficial effects. To test this idea, the effects of iron and copper on both the neuroprotective and anti-inflammatory activities of fisetin were examined. It is shown that while fisetin can reduce the potentiation of cell death by iron and copper in response to treatments that lower glutathione levels, it is much less effective when the metals are combined with other inducers of oxidative stress. In addition, iron but not copper reduces the anti-inflammatory effects of fisetin in a dose-dependent manner. These effects correlate with the ability of iron but not copper to block the induction of the antioxidant transcription factor, Nrf2, by fisetin. In contrast, although the flavanone sterubin also binds iron, the metal has no effect on sterubin’s ability to induce Nrf2 or protect cells from toxic or pro-inflammatory insults. Together, these results suggest that while iron and copper binding could contribute to the beneficial effects of neuroprotective compounds in the context of neurodegenerative diseases, the consequences of this binding need to be fully examined for each compound.

scholarly journals Antioxidant, anti-inflammatory and antimicrobial activities of aqueous and methanolic extract of Rosmarinus eriocalyx Jord. & Fourr.

2020 ◽  
Vol 14 (1) ◽  
pp. 254-262
Author(s):  
Nouioua Wafa ◽  
Gaamoune Sofiane
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Methanolic Extract ◽  
Reducing Power ◽  
Antimicrobial Activities ◽  
Stabilization Method ◽  
Membrane Stabilization ◽  
Anti Inflammatory

Rosmarinus eriocalyx Jord. & Fourr is a well-known aromatic and medicinal plant whose consumption serves to remedy a number of disorders, evergreen bush endemic to Algeria. The present study aimed it investigating the in-vitro anti-inflammatory and antimicrobial for two extracts of Rosmarinus eriocalyx. The antioxidant activity was evaluated by DPPH and the reducing power essay, anti-inflammatory activity with the Human Red Blood Cell (HRBC) membrane stabilization method. However, the antimicrobial activity was tested with three bacterial strain Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC25923 and Bacillus subtilis ATCC6633. The results show an excellent antioxidant and an interesting inflammatory activities but a weak power against the used strains therefore. © 2020 International Formulae Group. All rights reserved. Keywords: Rosmarinus eriocalyx Jord. & Fourr, DPPH, reducing power, HRBC, antimicrobial

scholarly journals INVESTIGATION ON PHARMACOGNOSY AS WELL AS THE ANTIOXIDANT, ANTI-INFLAMMATORY POTENTIAL OF THE KATHA POWDER

2021 ◽  
pp. 125-132
Author(s):  
PANKAJ SHARMA ◽  
RAJU L
Keyword(s):  
Diclofenac Sodium ◽  
In Vitro Study ◽  
Alcoholic Solution ◽  
Hot Water ◽  
Dependent Manner ◽  
Standard Drug ◽  
Anti Inflammatory ◽  
Acacia Catechu ◽  
Dose Dependent

Objective: The objective of the study was to investigate the pharmacognosy as well as the antioxidant, anti-inflammatory potential of the Katha powder. Methods: The Coarsely dried chips of Acacia catechu heartwood were treated with 10 % hydro-alcoholic solution to obtain Katha as the final product. The powdered Katha was standardized through pharmacognostic parameters. This Katha power is showing the good solubility in the hot water having astringent in the taste. The powder microscopy of the Katha powder is to be demonstrated fragments of acicular crystals, fibers, and bordered pitted vessels. Katha powder antioxidant potential is to be accessed by using the 2, 2-diphenyl-1-picryl hydrazyl assay and NO Scavenging assay using ascorbic acid as a standard drug. Further, the Katha powder is to be subjected for the assessment of its anti-inflammatory potential by the use of heat-induced hemolysis as well as hypotonicity-induced hemolysis approach by the use of the aspirin or diclofenac sodium as a standard drug. Results: Microscopical investigations were showed that Katha showing the presence of fragments of acicular crystals, fibers, and bordered pitted vessels. In vitro study shows that the Katha powder has excellent antioxidant as well as anti-inflammatory potential in a dose-dependent manner in comparison of the result of heartwood of A. catechu. Conclusion: So from this investigation, it is to be suggested that the Katha powder is rich in the phenolic compound and the experimentation study shows that the drug is to possess a good antioxidant as well as anti-inflammatory property.

scholarly journals Topical Anti-Inflammatory Activity of Essential Oils of Alpinia calcarata Rosc., Its Main Constituents, and Possible Mechanism of Action

2020 ◽  
Vol 2020 ◽  
pp. 1-19
Author(s):  
Madhuvanthi Chandrakanthan ◽  
Shiroma M. Handunnetti ◽  
Galbada Sirimal Arachchige Premakumara ◽  
Selvaluxmy Kathirgamanathar
Keyword(s):  
Skin Inflammation ◽  
Inflammatory Activity ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Proinflammatory Mediators ◽  
Mouse Ear ◽  
Anti Inflammatory ◽  
Dose Dependent ◽  
Topical Analgesic

This study aimed at investigating the anti-inflammatory potential of essential oil from rhizome and leaf of Alpinia calcarata Rosc. (ACEO) with the focus of its topical anti-inflammatory activity along with its dominant compounds 1,8-cineole and α-terpineol using mouse ear edema model. ACEOs were analyzed by GC-MS. The anti-inflammatory activity was determined by studying the inhibition of overproduction of proinflammatory mediators—nitric oxide, reactive oxygen species, prostaglandins, cyclooxygenases, and cytokines induced by lipopolysaccharides in murine macrophages. Topical anti-inflammatory and antinociceptive activity was studied by 12-O-tetradecanoylphorbol-13-acetate (TPA) induced skin inflammation and formalin-induced pain model in mice, respectively. Rhizome oil has 1,8-cineole (31.08%), α-terpineol (10.31%), and fenchyl acetate (10.73%) as major compounds whereas the ACEO from leaves has 1,8-cineole (38.45%), a-terpineol (11.62%), and camphor (10%). ACEOs reduced the production of inflammatory mediators in vitro in a concentration-dependent manner. Further, ACEO and its major compounds reduced ear thickness, weight, myeloperoxidase, and cytokines significantly (p<0.01) in mouse ear. Dose-dependent reduction in flinching and licking in both the phases of pain sensation concludes the topical analgesic effect. Our findings suggest the potency of topical use of ACEOs for inflammatory disease conditions.

The N-Terminal Domain of Thrombomodulin Sequesters HMGB1: A Novel Anti-Inflammatory Mechanism.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 3435-3435
Author(s):  
Kazuhiro Abeyama ◽  
Yasushi Yoshimoto ◽  
Ikuro Maruyama
Keyword(s):  
Protein C ◽  
Statistical Significance ◽  
Dependent Manner ◽  
Protective Effects ◽  
Binding Studies ◽  
Terminal Domain ◽  
Anti Inflammatory ◽  
Dose Dependent

Abstract Thrombomodulin (TM) is an endothelial anticoagulant cofactor that promotes thrombin-mediated formation of activated protein C (APC), the latter an enzyme with potent anti-coagulant and anti-inflammatory properties. We have found that the N-terminal, lectin-like domain (D1) of thrombomodulin has unique anti-inflammatory properties. Thrombomodulin, via D1, binds high mobility group-B1 DNA binding protein (HMGB1), a factor closely associated with necrotic cell damage following its release from the nucleus, thereby preventing leukocyte activation in vitro, and ultraviolet radiation-induced cutaneous inflammation and lipopolysaccharide-induced lethality in vivo. Our data also demonstrate anti-inflammatory properties of a peptide spanning the D1 domain of TM and suggest its therapeutic potential. These findings highlight a novel mechanism through which an endothelial cofactor, TM, suppresses inflammation; i.e., sequestration of mediators thereby preventing their interaction with cell surface receptors on effector cells in the vasculature. Results: TM binds HMGB1 and prevents expression of pro-inflammatory activity. Our co-culture studies of leukocytes and HUVEC, and results in the cutaneous irritation model suggested that early release of a mediator, such as HMGB1, might contribute importantly to cellular activation in inflammation at later time points. In this context, TM might have the ability to decrease HMGB1-mediated inflammatory events. Binding studies using surface plasmon resonance (SPR), performed to directly assess the interaction of TM and immobilized HMGB1, demonstrated dose-dependent binding in the nanomolar range (Kd ~232 nM). Furthermore, addition of rhs-TM decreased, in a dose-dependent manner, the binding of HMGB1 to RAGE through the its N-terminal domain, but not anti-coagulant domain. TM and the N-terminal-derived TM peptide have anti-inflammatory effects in settings where HMGB1 is a likely key mediator. In HMGB1-mediated skin inflammation model, systemic administration of rhs-TM, its lectin-like domain and sRAGE resulted in a significant blunting of the inflammatory response. In contrast, the effect of anti-coagulant domain, although showing a trend toward decreased ear swelling, did not achieve statistical significance (anticoagulant domain has anti-inflammatory effects in vivo that probably reflect its ability to support thrombin-mediated activation of protein C; the latter does not occur in vitro after inactivation of the protein C zymogen by heat treatment). In view of recent data suggesting a link between HMGB1 released from injured tissue and endotoxin-induced lethality in mice, we also tested whether rhs-TM and its lectin-like domain might also have protective effects in this model. We employed a dose of intraperitoneal (IP) LPS (10 mg/kg) resulting in 100% lethality by 96 hrs. Systemic (IP) treatment of animals with anti-HMGB1 IgY had a protective effect with respect to lethality at 4 days, whereas the same regimen of nonimmune IgY was without effect. Similarly, IP administration of rhs-TM and its N-teminal lectin domain, but not anti-coagulant domain had complete protective effects compared with anti-HMGB1 IgY. Conclusion: Our findings have elucidated an unexpected anti-inflammatory property of TM residing in the D1 domain, namely binding of HMGB1.

scholarly journals STUDY OF PARMELIA PERLATA FOR ITS POTENTIAL AS ANTI-INFLAMMATORY AND ANTIARTHRITIC AGENT USING IN VITRO MODEL

2019 ◽  
Vol 12 (1) ◽  
pp. 95 ◽  
Author(s):  
Yogesh Diwakar ◽  
Chitra V ◽  
Evelyn Sharon S
Keyword(s):  
Protein Denaturation ◽  
Inflammatory Activity ◽  
Maximum Effect ◽  
Dependent Manner ◽  
Membrane Stabilization ◽  
Human Red Blood Cells ◽  
Phytochemical Compounds ◽  
Anti Inflammatory ◽  
Vitro Model

Objective: The objective of this study was to evaluate the anti-inflammatory and antiarthritic potential of Parmelia perlata. Methods: The relative study is based on in vitro anti-inflammatory and antiarthritic activity using hydroalcoholic extract of P. perlata (HAEPP). The preliminary phytochemical tests showed the presence of various phytochemical compounds such as alkaloids, flavonoids, and glycosides since the lichen species of P. perlata has the folklore claim of anti-inflammatory activity, thus it was studied by human red blood cells membrane stabilization method, and arthritic activity was carried using protein denaturation method using diclofenac as a standard.Results: The results showed eminent anti-inflammatory and antiarthritic activity in a dose-dependent manner. The membrane stabilization showed the maximum effect at 78.54% at the concentration of 1000 μg/, and the protein denaturation was also found maximum at 1000 μg/ml concentration at 79.43%. Thus, our research states the potent anti-inflammatory activity and antiarthritic effect in P. perlata. Conclusion: The HEAPP has a potent anti-inflammatory activity and antiarthritic activity. A further study has to be conducted to establish the pharmacological evidence behind the compound and the mechanism of action of the HAEPP on the inhibition of the inflammation process.

scholarly journals Antioxidant, anti-inflammatory and anticoagulant activities of three Thymus species grown in southeastern Morocco

2019 ◽  
Vol 5 (1) ◽  
Author(s):  
Abdelbassat Hmidani ◽  
Eimad Dine Tariq Bouhlali ◽  
Tarik Khouya ◽  
Mhamed Ramchoun ◽  
Younes Filali-Zegzouti ◽  
...  
Keyword(s):  
Anticoagulant Activity ◽  
Activated Partial Thromboplastin Time ◽  
Dependent Manner ◽  
Thrombin Time ◽  
Thymus Zygis ◽  
Wide Range ◽  
Thymus Satureioides ◽  
Anti Inflammatory ◽  
Dose Dependent

Abstract Background Thyme has been used for centuries in southeastern Morocco to treat a wide range of diseases such as inflammation disorders. The aim of the current study is to examine and to compare in vitro the anti-inflammatory, antioxidant, and anticoagulant activities of three thyme species grown in southeastern Morocco. Results Data showed that all studied species possess an important antioxidant activity: Thymus atlanticus (IC50 = 16.59 μg/mL), Thymus zygis (IC50 = 15.43 μg/mL), and Thymus satureioides (IC50 = 14.65 μg/mL). Concerning the anti-inflammatory activity, the highest effect was depicted in Thymus atlanticus followed by Thymus zygis and Thymus satureioides. With regard to the anticoagulant activity, the aqueous extract of these species prolongs activated partial thromboplastin time, prothrombin time, and thrombin time significantly (p < 0.05) in a dose-dependent manner. Conclusion Our results provide evidence that thymus extract exhibits marked antioxidant, anticoagulant, and anti-inflammatory effects, thus justifying the popular uses of these plants to treat some inflammatory and cardiovascular illnesses.

scholarly journals Anti-Inflammatory Compounds from Vietnamese Piper bavinum

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Viet Dung Hoang ◽  
Phi Hung Nguyen ◽  
Minh Thu Doan ◽  
Manh Hung Tran ◽  
Nhu Tuan Huynh ◽  
...  
Keyword(s):  
Raw 264.7 Cells ◽  
No Production ◽  
Dependent Manner ◽  
Protein Levels ◽  
Chemical Structures ◽  
Anti Inflammatory ◽  
First Time ◽  
Dose Dependent ◽  
Potent Inhibitory Activity

This study reports the anti-inflammatory activity-guided fractionation of the aerial part of Piper bavinum C. CD. (Piperaceae) that led to the isolation of eight secondary metabolites (1–8). The chemical structures of 1–8 were established mainly by NMR and mass spectra. Compound 5 was isolated from P. bavinum for the first time. All the isolated compounds were evaluated against LPS-induced NO production in macrophage RAW 264.7 cells in vitro. Among them, compound 4 showed the most potent inhibitory activity against the LPS-induced NO production with an IC50 value of 5.2 μM followed by compound 5 that inhibited NO production with an IC50 value of 13.5 μM. In the protein levels, compound 4 suppressed LPS-induced COX-2 and iNOS expressions in a dose-dependent manner. The results suggested that P. bavinum and its constituents might exert anti-inflammatory effects.

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