Sub-chronic oral toxicity study of “Kien Nao Dan” tablets in experimental animal

2021 ◽  
Vol 148 (12) ◽  
pp. 16-23
Author(s):  
Trinh Thi Thuy Hong ◽  
Le Thanh Xuan ◽  
Tran Quang Minh ◽  
Vu Viet Hang ◽  
Pham Thi Van Anh ◽  
...  
Keyword(s):  
Cerebral Ischemia ◽  
Oral Administration ◽  
Wistar Rats ◽  
Chronic Toxicity ◽  
Physiological Changes ◽  
Biological Parameters ◽  
Oral Toxicity ◽  
Traditional Medicines ◽  
Human Dose ◽  
Histopathologic Analysis

“Kien nao dan” (KND) tablet is composed of 13 traditional medicines that may has preventive and effective treatment of cerebral ischemia. However, there are no scientific reports of its toxicological properties which guarantee of the safety its usage treatment. Therefore, the aim of this study was to investigate the sub-chronic toxicity of KND tablet on rats through oral administration. The sub-chronic toxicity was evaluated by the recommendation of WHO in Wistar rats at doses of 0.72 g/kg/day (equal to recommended human dose) and 2.16 g/kg/day (3 times as high as recommended human dose) for 8 consecutive weeks. In the evaluation of sub-chronic toxicity, there were no behavioral and physiological changes or signs of toxicity. The result of the hematological and biological parameters after administration of KND tablets showed no change. The histopathologic analysis of livers and kidneys indicated that no significant differences were observed between the exposed and unexposed rat groups. In conclusion, “Kien nao dan” tablets did not produce sub-chronic toxicity in Wistar rats.

Sub-chronic oral toxicity study of “Phong Thap Dan” tablets in experimental animal

2021 ◽  
Vol 148 (12) ◽  
pp. 24-31
Author(s):  
Le Thanh Xuan ◽  
Le Thi Nhat Ngoc ◽  
Tran Quang Minh ◽  
Vu Viet Hang ◽  
Pham Thi Van Anh ◽  
...  
Keyword(s):  
Oral Administration ◽  
Experimental Animal ◽  
Wistar Rats ◽  
Chronic Toxicity ◽  
Toxicity Study ◽  
Biological Parameters ◽  
Oral Toxicity ◽  
Human Dose ◽  
Significant Difference ◽  
Experimental Group

The present study aimed to investigate the sub-chronic toxicity of “Phong thap dan” (PTD) tablets through oral administration in experimental animal. The sub-chronic toxicity was evaluated by the WHO recommendation in Wistar rats at doses of 0.72 g/kg/day (equal to recommended human dose) and 2.16 g/kg/day (3 times as high as recommended human dose). In the sub-chronic experimental group, the PTD was administered orally daily for 8 consecutive weeks. In the evaluation of sub-chronic toxicity, there were no behavioral and physiological change or sign of toxicity. The result of the hematological and biological parameters after administration of PTD tablets showed no change. The histopathology analysis of livers and kidneys indicated that no significant difference was observed between the exposed and unexposed rat groups. In conclusion, “Phong thap dan” tablets did not produce sub-chronic toxicity in Wistar rats.

scholarly journals Evaluation of 90-day subchronic oral toxicity of aqueous extract of Gmelina arborea Roxb (Verbenaceae) leaves in Wistar rats

2020 ◽  
Vol 19 (3) ◽  
pp. 617-622
Author(s):  
Razack Osseni ◽  
Azonbakin Simon ◽  
Diallo Aboudoulatif ◽  
Habib Ganfon ◽  
Adjagba Marius ◽  
...  
Keyword(s):  
Food Intake ◽  
Aqueous Extract ◽  
Wistar Rats ◽  
The Body ◽  
Biological Parameters ◽  
Gmelina Arborea ◽  
Oral Toxicity ◽  
Subchronic Toxicity ◽  
Significant Difference ◽  
Body Weights

Purpose: To evaluate the 90 day sub-chronic toxicity of aqueous extract of Gmelina arborea leaves in Wistar rats. Methods: Rats were submitted to repeated daily oral administration of extract (250, 62.5 and 15.62 mg/kg) of Gmelina arborea leaves. The control groups were given distilled water and the rats were monitored for any toxicity symptoms as well as body and organs weights, water and food intake changes. The biochemical, haematological and histolopathological parameters were analysed. Results: The 90 days administration of the aqueous extract did not produce any toxicity signs or mortality. In addition, no significant alteration in water or food intake by the rats was observed. Although there were no changes in the body weights, significant decrease in the weight of the kidneys of the rats was observed at 250 mg/kg. Biological parameters as well as the histopathology of liver and kidneys were not significantly affected. Significant decreases were noted in glucose level at the three dose levels. In addition, significant difference in the levels of transaminases, glucose and platelets were observed. Conclusion: The 90-days subchronic toxicity test on Gmelina arborea did not produce any toxic effects. This confirms the safety of the plant leaves by traditional medicine practitioners. Keywords: Gmelina arborea, Subchronic toxicity, Wistars rats, Biological parameters

scholarly journals Ethyl acetate fraction of Psorospermum febrifugum Spach aqueous extract did not exhibit acute or sub-chronic toxicity. Experimental study on Wistar rats

2019 ◽  
Vol 11 (4) ◽  
pp. 123
Author(s):  
F Agbogba ◽  
M Sènou ◽  
AP Tchogou ◽  
JE Lokonon ◽  
TI Sacramento ◽  
...  
Keyword(s):  
Body Weight ◽  
Ethyl Acetate ◽  
Aqueous Extract ◽  
Toxicity Test ◽  
Wistar Rats ◽  
Chronic Toxicity ◽  
Ethyl Acetate Fraction ◽  
Root Bark ◽  
Oral Toxicity ◽  
Function Tests

Psorospermum febrifugum Spach (Clusiaceae) was a tropical plant whose root bark was used to treat anemia. This work aimed to evaluate the safety of the ethyl acetate fraction of the aqueous extract of this bark. Methods: The ethyl acetate fraction of the extract was administered to Wistar rats in a single dose of 2000 mg / Kg body weight for acute oral toxicity test or daily doses of 200 mg / Kg of body weight during 28 days for sub-chronic oral toxicity test, as recommended by the OECD. At day 0, then at day 14 for the acute phase and day 28 for the sub-chronic phase, the rats were weighed and their blood collected for tests. The activity of transaminases AST and ALT were measured in the liver function tests, blood urea and creatinine were measured for renal function tests and blood leukocytes were counted for the immune balance. These analyzes were supplemented by the histology of the liver, kidneys and spleen, an immune organ. Results: In acute and sub-chronic oral toxicity tests, rat’s weight, liver, kidney and immune balances as well as these organs histology were not affected, suggesting the safety of the extract fraction. Conclusion: The ethyl acetate fraction of the aqueous extract of the root bark of Psorospermum febrifugum did not reveal any acute or sub-chronic oral toxicity. This effect could be related to its richness in flavonoids which have cytoprotective effects. The study of biological tolerance deserves to be continued by the chronic toxicity test and appropriate clinical trials.

The evaluation of acute and subchronic toxicities of An Phu Khang capsules in experimental animals

2021 ◽  
Vol 148 (12) ◽  
pp. 86-95
Author(s):  
Ha Thi Yen ◽  
Tran Thanh Tung ◽  
Dang Thi Thu Hien
Keyword(s):  
Acute Toxicity ◽  
Oral Administration ◽  
Wistar Rats ◽  
Hematological Parameters ◽  
Control Group ◽  
Subchronic Toxicity ◽  
Experimental Animals ◽  
Swiss Mice ◽  
Human Dose ◽  
Liver And Kidney

The purpose of this research was to evaluate the acute and subchronic toxicities of An Phu Khang capsules through oral administration in experimental animals. The acute toxicity was determined by the method of Litchfield Wilcoxon in Swiss mice. The subchronic toxicity was evaluated by the recommendation of WHO in Wistar rats at these doses of 0.54 g/kg b.w/day (equal to recommended human dose) and 1.62 g/kg b.w/day (3 times as high as recommended human dose) in 4 consecutive weeks. As a result, An Phu Khang capsules at the highest dose used for mice (36.29 g/kg b.w) did not show acute toxicity in mice. In terms of the subchronic toxicity test, after oral administration of An Phu Khang capsules, hematological parameters, hepato-renal functions, and microscopic images of liver and kidney at both doses were unchanged compared with the control group. In conclusion, An Phu Khang with both doses 0.54 g/kg b.w/day and 1.62 g/kg b.w/day did not produce acute and subchronic toxicities in Swiss mice and Wistar rats.

scholarly journals A botanical from the antiproliferative Cameroonian spice, Imperata cylindrica is safe at lower doses, as demonstrated by oral acute and sub-chronic toxicity screenings

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Paul Nayim ◽  
Armelle T. Mbaveng ◽  
Arsene M. Ntyam ◽  
Victor Kuete
Keyword(s):  
Oral Administration ◽  
Chronic Toxicity ◽  
Human Cancer ◽  
Imperata Cylindrica ◽  
Female Rats ◽  
Mean Corpuscular Hemoglobin ◽  
Root Extract ◽  
Oral Toxicity ◽  
Human Cancer Cell Lines ◽  
Male And Female Rats

Abstract Background The cytotoxicity of the root’s methanol extract of Imperata cylindrica (ICR). was previously reported in a panel of human cancer cell lines, including multi-drug resistant phenotypes. The aim of this study was to assess the acute and sub-chronic oral toxicity of methanol root extract of Imperata cylindrica. Methods The acute toxicity was carried out according to the experimental protocol of OECD. The plant extract was administered orally to female rats at a single dose of 5000 mg/kg for 14 days and the animals were observed for any behavioral changes or mortality. For sub-chronic toxicity study, ICR was orally administered daily to male and female rats at different doses (250, 500 and 1000 mg/kg per b.w.) for 30 days. During these treatment days the animals were observed for any appearance of toxicity symptoms; following the treatment period, animals were sacrificed for hematological, biochemical and histopathology analysis. Results From the results of the acute oral toxicity assay, ICR was found to be non-toxic at the dose of 5000 mg/kg b.w. During the period of sub-chronic toxicity test, observation of signs, behavior and health status of the animals showed no abnormality in the groups of animals treated with ICR as compared to the controls. Significant variation of the relative body weights of heart and kidney were observed at dose a 1000 mg/kg b.w. Significant decrease of aspartate aminotransferase, creatinine level, low density lipoprotein concentration, triglyceride and total cholesterol were observed. In males, we noticed a significant decrease of the level of granulocytes with an increase of lymphocytes and mean corpuscular hemoglobin concentration levels. Histological examinations performed on kidney and liver showed a normal kidney architecture and liver also presented a normal hepatic architecture with slight degeneration at a dose 1000 mg/kg b.w. Conclusion ICR is safe for acute oral administration; however, for long-term oral administration, safety measures should be taken. Thus, oral sub-chronic exposure of ICR at lower doses are recommended while higher doses around 1000 mg/kg b.w. should be discouraged.

scholarly journals In Vivo Evaluation of the Chronic Oral Toxicity of the Marine Toxin Palytoxin

Toxins ◽  
2020 ◽  
Vol 12 (8) ◽  
pp. 489
Author(s):  
Andrea Boente-Juncal ◽  
Sandra Raposo-García ◽  
Carmen Vale ◽  
M. Carmen Louzao ◽  
Paz Otero ◽  
...  
Keyword(s):  
Oral Administration ◽  
Chronic Toxicity ◽  
Lethal Dose ◽  
Oral Toxicity ◽  
In Vivo Evaluation ◽  
Marine Toxin ◽  
Adverse Effect Level ◽  
Marine Products ◽  
Effect Level

Palytoxin (PLTX) is one of the most poisonous substances known to date and considered as an emergent toxin in Europe. Palytoxin binds to the Na+-K+ ATPase, converting the enzyme in a permeant cation channel. This toxin is known for causing human fatal intoxications associated with the consumption of contaminated fish and crustaceans such as crabs, groupers, mackerel, and parrotfish. Human intoxications by PLTX after consumption of contaminated fishery products are a serious health issue and can be fatal. Different reports have previously explored the acute oral toxicity of PLTX in mice. Although the presence of palytoxin in marine products is currently not regulated in Europe, the European Food Safety Authority expressed its opinion on PLTX and demanded assessment for chronic toxicity studies of this potent marine toxin. In this study, the chronic toxicity of palytoxin was evaluated after oral administration to mice by gavage during a 28-day period. After chronic exposure of mice to the toxin, a lethal dose 50 (LD50) of 0.44 µg/kg of PLTX and a No-Observed-Adverse-Effect Level (NOAEL) of 0.03 µg/kg for repeated daily oral administration of PLTX were determined. These results indicate a much higher chronic toxicity of PLTX and a lower NOAEL than that previously described in shorter treatment periods, pointing out the need to further reevaluate the levels of this compound in marine products.

scholarly journals Evaluation of acute and subchronic toxicities of “Phuong Dong Dai Trang” tablets in experimental animals

2021 ◽  
Vol 141 (5) ◽  
pp. 29-38
Author(s):  
Tran Thanh Tung ◽  
Dau Thuy Duong ◽  
Pham Thi Thuy Minh ◽  
Nguyen Thu Hien ◽  
Dinh Thi Thu Hang
Keyword(s):  
Acute Toxicity ◽  
Oral Administration ◽  
Wistar Rats ◽  
Hematological Parameters ◽  
Control Group ◽  
Subchronic Toxicity ◽  
Swiss Mice ◽  
Human Dose ◽  
Liver And Kidney ◽  
Oral Doses

The study aimed to evaluate the acute and subchronic toxicities of “Phuong Dong Dai Trang” tablets through oral administration using experimental animal models. Acute toxicity in Swiss mice was determined using the Litchfield Wilcoxon method. The subchronic toxicity in Wistar rats was evaluated according to WHO and OECD’s recommendation with oral doses of 4.68 g/kg/day (equivalent to recommended human dose) and 14.04 g/kg/day (3 times the recommended human dose) for 4 consecutive weeks. In terms of acute toxicity, “Phuong Dong Dai Trang” tablets did not express acute toxicity in mice at the highest dose used (232.14 g materials/kg). In terms of the subchronic toxicity, after oral administration of “Phuong Dong Dai Trang” tablets, hematological parameters, hepato - renal functions, and microscopic images of liver and kidney were unchanged in the treatment group compared to the control group. In conclusion, “Phuong Dong Dai Trang” tablets did not produce acute and subchronic toxicities in Swiss mice and Wistar rats.

scholarly journals Chronic Toxicity Study of Aqueous Roots Extract of Rauvolfia vomitoria AFZEL on Haematological Parameters in Wistar Rats

2021 ◽  
Vol 10 (9) ◽  
pp. 567-577
Author(s):  
Roland Patrick N Cho Mama Koné ◽  
Moussa Gbogbo N Guessan Jean Baptiste Oussou ◽  
Angoué Paul Yapo
Keyword(s):  
Oral Administration ◽  
Therapeutic Dose ◽  
Wistar Rats ◽  
Chronic Toxicity ◽  
Haematological Parameters ◽  
High Dose ◽  
Dependent Manner ◽  
Delayed Effects ◽  
Dose Dependent ◽  
Rauvolfia Vomitoria

Rauvolfia vomitoria Afzel. (Apocynaceae) is a plant used in traditional medicine in Côte d'Ivoire for the treatment of several conditions. This work aims to evaluate the effects of repeated oral administration of the aqueous roots extract Rauvolfia vomitoria AFZEL (AERv) on haematological parameters in Wistar rats. Thus, 100 healthy rats were divided into four groups (T, A, B, C) of 20 rats each. The sub groups Ts and Cs from the groups T and C included 10 rats (5 males and 5 females) were formed for a reversibility study. After 6 months (180 days) of the experiment, the sub groups rats Ts and Cs were no more treated with the extract till the 210th day (7 months), day of their sacrifice to verify the reversibility and/or delayed effects of AERv. The erythrocyte, platelet and leukocyte parameters were determined. Results showed that AERv caused a significant decrease in erythrocyte and thrombocyte parameters with the high dose (1000 mg/kg bw) while an increase in leukocyte parameters was noted in a dose-dependent manner. The aqueous extract of the barkless roots is not harmful at the therapeutic dose of 700 mg/kg bw on the haematological parameters. After stoping administration the effects were reversible.

scholarly journals The 90-day oral toxicity of D-psicose in male Wistar rats

2012 ◽  
Vol 50 (2) ◽  
pp. 158-161 ◽  
Author(s):  
Tatsuhiro Matsuo ◽  
Reika Ishii ◽  
Yoko Shirai
Keyword(s):  
Wistar Rats ◽  
Oral Toxicity ◽  
Male Wistar Rats

scholarly journals RESVERATROL INCLUSION COMPLEX WITH β-CYCLODEXTRIN (RCD): CHARACTERIZATION AND EVALUATION OF TOXICITY IN WISTAR RATS

2016 ◽  
Vol 9 (1) ◽  
pp. 27 ◽  
Author(s):  
V. S. K. Nishihira ◽  
N. J. Mezzomo ◽  
M. D. Baldissera ◽  
R. A. Vaucher ◽  
C. G. Pinto ◽  
...  
Keyword(s):  
Inclusion Complex ◽  
Oral Administration ◽  
Biochemical Parameters ◽  
Wistar Rats ◽  
Metabolic Diseases ◽  
Hematological Parameters ◽  
Density Lipoprotein ◽  
Blood Parameters ◽  
Control Group ◽  
Scanning Calorimetry

<p class="RSCB01ARTAbstract"><strong>Objective</strong>:<strong> </strong>The aim of this study was to characterise the resveratrol inclusion complex with β-cyclodextrin (RCD) and evaluate their toxicity in wistar rats.</p><p class="RSCB01ARTAbstract"><strong>Methods: </strong>The RCD were prepared in ultra-turrax. For characterization of the RCD were used: Fourier transform infra-red Spectroscopy, Nuclear Magnetic Resonance (NMR), Differential Scanning Calorimetry (DSC) and X-ray powder diffraction. The RCD and others 4 treatments were performed by the chronic oral administration in 35 rats during 60 ds. After the treatments they were euthanized and the serum blood were collected to analyzed some hemogram and biochemical parameters including aspartyl aminotransferase (AST); alanine aminotransferase (AST); phosphatase alkaline (ALP); total bilirubin (TB); direct bilirubin (DB); total protein (TP); total cholesterol (TC), triacylglycerol (TAG), very low-density lipoprotein (VLDL), high-density lipoprotein (HDL), calcium, iron and phosphate using fully automated biochemistry analyzer.</p><p class="RSCB01ARTAbstract"><strong>Results: </strong>The characterization results indicated a successful formation of the RCD. All hematological parameters analysed were within the normal values in all the groups. Furthermore, the hemogram and biochemical parameters were significantly (P&gt;0.05) similar to the control group.</p><p class="RSCB01ARTAbstract"><strong>Conclusion: </strong>The daily oral administration during 60 d of RCD are not harmful on blood parameters of Wistar rats. Thus, RCD can be used safely for treatment of some metabolic diseases.</p>

Sign in / Sign up

Export Citation Format

Share Document