scholarly journals Alpha-Lipoic Acid: Antioxidant Activity Against Non-Enzymatic Peroxidation of Rat Kidney and Liver Mitochondria

2021 ◽  
pp. 1-4
Author(s):  
Mariana Gavazza ◽  
Mónica Marmunti ◽  
Patricio Leaden ◽  
Pedro Zeinsteger ◽  
Alejandro Palacios
Keyword(s):  
Lipoic Acid ◽  
Light Emission ◽  
Cell Damage ◽  
Rat Kidney ◽  
Control Group ◽  
Alpha Lipoic Acid ◽  
Prosthetic Group ◽  
Antioxidant Power ◽  
Kidney Mitochondria ◽  
Liver And Kidney

Reactive Oxygen Species (ROS) participate in the induction and progression of damage in many human pathologies, such as: heart attack, cerebral ischemia, diabetic neuropathy and Alzheimer's disease, among others. Alpha Lipoic Acid (ALA, also called thioctic acid) is a sulfur compound that acts as a growth factor in some microorganisms and as a coenzyme or prosthetic group in mammalian tissues. The beneficial action of ALA is due to its high antioxidant power that allows it to capture numerous free radicals such as Hydroxyl Radicals (OH•), Hypochlorous (HClO-) and oxygen (O•). ALA easily crosses cell membranes acting in both lipophilic and hydrophilic media, so it can act against oxidative stress and prevent cell damage at many levels. In the study reported here the effect of ALA on chemiluminescence of mitochondria isolated from liver and kidney rat was analyzed. After incubation of both mitochondria in an ascorbate (0.4mM)-Fe++ (2.15μM) system (120min at 37°C), non-enzymatic peroxidation, it was observed that the total cpm/mg protein originated from light emission: chemiluminescence was lower in liver and kidney mitochondria obtained from ALA group than in the control group (without ALA). Moreover, it was observed that the ALA was reduced, concentration dependent (0.05 mg, 0.15 mg and 0.25 mg of solution), of chemiluminescence, measured as total cpm. The analyses of chemiluminescence indicate that ALA may act as antioxidant protecting rat liver and kidney mitochondria from peroxidative damage.

scholarly journals Effect of In-Vitro Alpha Lipoic Acid Addition on Spermatozoa Motility in Sperm Preparation Process

2021 ◽  
Vol 55 (4) ◽  
pp. 246
Author(s):  
Gede Wira Buanayuda ◽  
Hamdani Lunardhi ◽  
Indra Gusti Mansur
Keyword(s):  
Lipoic Acid ◽  
Intrauterine Insemination ◽  
Control Group ◽  
Infertile Couple ◽  
Preparation Process ◽  
Alpha Lipoic Acid ◽  
Sperm Preparation ◽  
Progressive Motility ◽  
Child Hospital

Infertility is a problem for husband and wife, in the last 20 years the number of infertile couples has tended to increase by around 6.5 million pairs. The infertile couple can use the intrauterine insemination method to obtain offspring if a conventional method approach cannot be performed. Insemination requires a sperm preparation stage in which there are centrifugation and resuspension procedures that tend to produce excess reactive oxygen species (ROS). Excessive ROS will damage the motility of the spermatozoa. This study aims to prove the addition of alpha lipoic acid (ALA) as an antioxidant in the process of sperm preparation to improve and maintain better sperm motility. This research is a laboratory study with an experimental research design. The sample consisted of 10 infertile men who visited the Andrology section of the Sayyidah Jakarta Mother and Child Hospital (RSIA), where each ejaculate from the patient would be divided into 3 groups namely (k1) fresh semen as a control group, (k2) sperm preparation group without ALA, (k3) group of sperm preparation with the addition of ALA. The motility of spermatozoa was observed with the WHO 1999 method for 4 hours in units of percent. Progressive motility in k3 (47.95 ± 3.617) was higher than in k2 (38.05 ± 3.278) statistically significantly different after 3 hours of observation (p<0.0001). Progressive motility in k3 (78.8 ± 5.841) was higher than k1 (56.55 ± 7.511) from the initial observation (p <0.0001). The progressive motility of k2 (76.05 ± 6.768) was higher than k1 (56.55 ± 7.511) from the start of the observation (0.0001). It can be concluded that the addition of ALA in the sperm preparation process increases and maintains progressive motility that is better than sperm preparation without ALA addition after 3 hours of observation.

scholarly journals Alpha-Lipoic Acid Protects Rat Kidney Against Oxidative Stress-Mediated DNA Damage and Apoptosis Induced by Lead

2015 ◽  
Vol 6 (1) ◽  
pp. 1-14 ◽  
Author(s):  
Samy Ali Hussein ◽  
Mohamed Ragaa R. Hassanein ◽  
Aziza Amin ◽  
Asmaa H. Mohammad Hussein
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
Lipoic Acid ◽  
Rat Kidney ◽  
Alpha Lipoic Acid

scholarly journals Effect of alpha-lipoic acid on antioxidant gene expression and kidney injury in alloxan-induced diabetic rats

2018 ◽  
Vol 8 (1) ◽  
pp. 6-6 ◽  
Author(s):  
Parisa Jamor ◽  
Hassan Ahmadvand ◽  
Hesam Ashoory ◽  
Esmaeel Babaeenezhad
Keyword(s):  
Gene Expression ◽  
Lipoic Acid ◽  
Kidney Injury ◽  
Diabetic Rats ◽  
Pcr Analysis ◽  
Control Group ◽  
Diabetic Patients ◽  
Alpha Lipoic Acid ◽  
The Impact ◽  
Gpx Activity

Background: Myeloperoxidase (MPO) is involved in the initiation, progression, and complications of atherosclerosis in diabetic patients. Objectives: In the current study, the impact of alpha-lipoic acid (LA), a natural antioxidant and a cofactor in the enzyme complexes on MPO, catalase (CAT) and glutathione peroxidase (GPx) activity, glutathione (GSH) and malondialdehyde (MDA) level, histopathology of kidney and expression of antioxidant enzymes, superoxide dismutase (SOD), GPx and CAT which are involved in the detoxification of reactive oxygen species (ROS), was evaluated in alloxan-induced diabetic rats. Materials and Methods: In this study, 30 male Rattus norvegicus rats randomly divided into three groups; control (C), non-treated diabetic (NTD), and LA-treated diabetics (LATD) was induced by alloxan monohydrate (100mg/kg; subcutaneous [SC]). Then treatment was performed with alphaLA (100 mg/kg intraperitoneal (i.p) daily to 6 weeks). Blood sample of animals collected to measure levels of MPO, CAT and GPx activity GSH and MDA. Kidney paraffin sections were prepared to estimate histological studies and to measure quantitative gene expression SOD, GPX and CAT in kidney. Results: Induction of diabetes led to a significant increase in MPO and MDA, reduced GSH level and GPx and CAT activities (P < 0.05). However, treatment with alpha-LA led to a significant elevation in GPx, CAT and GSH levels with a reduction in MPO activities and MDA levels (P < 0.05). Furthermore, the real-time reverse transcriptase-polymerase chain reaction (RT-PCR) analysis results showed increased expressions of GPx, CAT and SOD enzyme in the treatment group compared with the diabetic control group. Histopathological lesions such as increased glomerular volume and lymphocyte infiltration were attenuated in the alpha-LA treated group. Conclusions: Our findings indicated that alpha-LA supplementation is effective in preventing complications induced by oxidative stress and atherosclerosis in diabetic rats.

Effect of alpha-lipoic acid on boar spermatozoa quality during freezing–thawing

Zygote ◽  
2015 ◽  
Vol 24 (2) ◽  
pp. 259-265 ◽  
Author(s):  
Tao Shen ◽  
Zhong-Liang Jiang ◽  
Cong-Jun Li ◽  
Xiao-Chen Hu ◽  
Qing-Wang Li
Keyword(s):  
Lipoic Acid ◽  
Artificial Insemination ◽  
Antioxidant Effect ◽  
Control Group ◽  
Antioxidant Enzyme Activities ◽  
Alpha Lipoic Acid ◽  
Glutamic Oxaloacetic Transaminase ◽  
Boar Semen ◽  
Endogenous Antioxidant ◽  
Boar Spermatozoa

SummaryAlpha-lipoic acid (ALA) is known to be a natural antioxidant. The aim of the present study was to evaluate the cryoprotective effect of ALA on the motility of boar spermatozoa and its antioxidant effect on boar spermatozoa during freezing–thawing. Different concentrations (2.0, 4.0, 6.0, 8.0 or 10.0 mg/ml) of ALA were added to the extender used to freeze boar semen, and the effects on the quality and endogenous antioxidant enzyme activities of frozen–thawed spermatozoa were assessed. The results indicated that the addition of ALA to the extender resulted in a higher percentage of motile spermatozoa post-thaw (P < 0.05). The activities of superoxide dismutase, lactate dehydrogenase, glutamic-oxaloacetic transaminase and catalase improved after adding ALA to the extender (P < 0.05). Artificial insemination results showed that pregnancy rate and litter size were significantly higher at 6.0 mg/ml in the ALA group than in the control group (P < 0.05). In conclusion, ALA conferred a cryoprotective capacity to the extender used for boar semen during the process of freezing–thawing, and the optimal concentration of ALA for the frozen extender was 6.0 mg/ml.

scholarly journals Oxidative stress and liver damage in birds exposed to diclofenac and lead

2014 ◽  
Vol 83 (4) ◽  
pp. 299-304 ◽  
Author(s):  
Jitka Osičková ◽  
Hana Banďouchová ◽  
Veronika Kováčová ◽  
Jiří Král ◽  
Ladislav Novotný ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Japanese Quail ◽  
Liver Damage ◽  
Multiple Stressors ◽  
Thiobarbituric Acid ◽  
Control Group ◽  
Toxic Substances ◽  
Exposure Group ◽  
Antioxidant Power ◽  
Liver And Kidney

Responses of wildlife to multiple stressors fit in the ecological concept of trade-off. While toxicity of non-steroidal anti-inflammatory drugs and heavy metals for free-ranging birds has been shown in single exposures, the present study aims to evaluate oxidative stress, and liver and kidney damage caused by single and combined effects of diclofenac and lead in the Japanese quail. Forty Japanese quail (Coturnix coturnix japonica) were divided into equal groups of controls, diclofenac, Pb, and Pb+diclofenac exposures. The birds were exposed to the respective chemicals through insertion of lead shots (1.5 g) into the crop on day 0 of the experiment and/or administration of 5 mg/kg of diclofenac intramuscularly in two treatments on days 0 and 5. Groups in liver and kidney tissues of birds were then compared after 10 days using histopathology and biochemistry markers such as glutathione reductase (GR), ferric reducing antioxidant power (FRAP), and lipid peroxidation measured as total thiobarbituric acid reactive species (TBARS). The liver damage score gradient was Pb+diclofenac exposure group > Pb exposure group > diclofenac exposure group and hepatic TBARS values were significantly increased in the group of birds exposed to a combination of diclofenac and lead compared to the healthy control group. The study has shown that, apart from the reported nephrotoxicity of diclofenac, hepatic toxicity should also be considered. Avian clinicians should be cautious when selecting drugs for therapy of wild birds with unknown history of exposure to toxic substances.

scholarly journals Clinical evaluation of the effectiveness of treatment of the hand-foot syndrome – skin toxicity of antitumor target therapy with multi-kinase inhibitors

2018 ◽  
Vol 12 (3) ◽  
pp. 140-149 ◽  
Author(s):  
Evgeniya A. Shatokhina ◽  
Larisa S. Kruglova ◽  
Oleg A. Shukhov
Keyword(s):  
Lipoic Acid ◽  
Kinase Inhibitors ◽  
Clinical Manifestations ◽  
Main Group ◽  
Betamethasone Dipropionate ◽  
Control Group ◽  
Positive Trend ◽  
Alpha Lipoic Acid ◽  
Significant Difference ◽  
Hand Foot Syndrome

Background. The effective treatment of the hand-foot syndrome is a necessary component of the complex treatment of patients receiving antitumor therapy with multikinase inhibitors. Aims. The assessment of a new effective method for the treatment of palm-plantar syndrome in patients receiving multi-kinase inhibitors. Materials and methods. There are 27 patients received antitumor treatment with multi-kinase inhibitors and had clinical manifestations of the hand-foot syndrome (HFS) for observation. The main group (14 patients) used a combination of an alpha-lipoic acid at a dose of 600 mg per day per os and an ointment containing 0.005% calcipotriol and 0.05% betamethasone dipropionate 2 times a day. The control group, included 13 patients, used a combination with a cream containing 10% urea and ointment containing 0.05% betamethasone dipropionate. Results. After a week of treatment, the regression of the skin process was observed in both groups, but it was more evident in the main group: significant differences in severity of erythema, paresthesia, pain and burning sensation, the Dermatological Life Quality Index (DLQI) were observed at this stage of therapy (p < 0.05). After 2 weeks of therapy: a more positive trend in the main group in terms of erythema, paresthesia and burning, but the most significant difference is DLQI – 17.4 in the main group and 22.8 in the control group (p < 0.0001). At the end of the 3rd week of therapy: differences with the main characteristics of erythema, desquamation, paresthesia (p < 0.0008), pain (p < 0.0001), DLQI (p < 0.0001). At the end point of the study (after 4 weeks of therapy), significant differences were found in reducing the symptoms and the main parameters for evaluating the effectiveness of DLQI in patients of the main group with a reliability of p < 0.0001. The high correlation of DLQI was found with erythema, pain, burning, paresthesia and desquamation. Conclusions. The treatment of the hand-foot plantar syndrome combined using alpha-lipoic acid inside and topical therapy in the form of an ointment containing calcipotriol and betamethasone dipropionate, can be recommended for patients receiving targeted therapy with multi-kinaseinhibitors.

scholarly journals ALPHA–LIPOIC ACID ANTIOXIDANT EFFICIENCY IN TREATMENT OF PERITONEAL DIALYSIS PATIENTS

2015 ◽  
pp. 42-48
Author(s):  
L. Korol ◽  
N. Stepanova ◽  
O. Ablogina ◽  
L. Migal
Keyword(s):  
Peritoneal Dialysis ◽  
Lipoic Acid ◽  
Negative Influence ◽  
Control Group ◽  
Alpha Lipoic Acid ◽  
Antioxidant Efficiency ◽  
Sh Groups ◽  
Before And After ◽  
Total Peroxidase Activity ◽  
Oxidative Processes

The aim of the study was to evaluate the effectiveness of the antioxidant alpha–lipoic acid in peritoneal dialysis (PD) patients taking into account the state of lipid peroxidation (LPO) and antioxidant protection (AOP) before and after treatment. Patients and methods. The research revealed the intensity of oxidative processes in 20 patients treated with continuous ambulatory peritoneal dialysis (CAPD) on the basis of а–lipoic acid applied parenterally in a dose of 600 mg for 2–weeks followed by a transfer to the 6 weeks of oral reception. The intensity of oxidative processes was evaluated before and after treatment through determining the content of MDA in serum (MDAs) and erythrocytes (MDAe). AOP activity was evaluated by the content of the antioxidant enzyme ceruloplasmin (CP), transferrin (Tr), SH–groups and total peroxidase activity (TPA) of red blood cells. The control group consisted of30 individuals comparable on for age and sex. Results. The effects on the study of PD patients (positive decrease of MDAs, increase in CP higher than control group, a significant increase in the content of SH–groups (p=0,04), TPA (p=0,009), Tr (p=0,002) and catalase serum) demonstrated a considerable positive dynamics of LPO /AOP influenced by the use of а–lipoic acid. Conclusions. The research opens up the prospect for further study ofLPO /AOP blood in PD patients and proves the application expediency of alpha–lipoic acid for this group. Use of the latter lowers the activity of oxidative processes significantly and restores AOP indicators substantially, which in turn minimizes the negative influence of oxidative stress on a patient’s body.

scholarly journals The effect of alpha-lipoic acid supplementation and Electrical isotonic contraction on anthropometric measurements, body composition, angiogenesis factors, sirtunin-1, and PGC1-α in obese people undergoing a weight loss diet

2020 ◽  
Author(s):  
Majid Mohammadshahi ◽  
Elahe Zakizadeh ◽  
Kambiz Ahmadi Angali ◽  
Majid Ravanbakhsh ◽  
Bijan Helli
Keyword(s):  
Weight Loss ◽  
Body Composition ◽  
Lipoic Acid ◽  
Anthropometric Measurements ◽  
Fat Free Mass ◽  
Control Group ◽  
Alpha Lipoic Acid ◽  
Anthropometric Parameters ◽  
Obese People ◽  
Isotonic Contraction

Abstract Background: The anti-obesity effects of Alpha-lipoic acid (α-LA) and isotonic contraction has been reported. However, the underlying mechanism is not fully understood. This study aimed to investigate the effect of α-LA supplementation and Faradic (an electrical stimulating system) on anthropometric parameters, body composition, VEGF, Sirtunin-1, nitric oxide (NO), and PGC1-α in obese people undergoing a weight loss regime.Methods: This randomized clinical trial was carried out on 100 obese adults. The subjects were randomly assigned to four groups of 25 subjects including Faradic, α-LA, -α-LA+Faradic, and control. A Bio Impedance Analyzer (BIA) was used to estimate anthropometric measurements including weight, body mass index (BMI), fat mass, and fat free mass. The serum levels of Sirtunin-1, PGC1-α, VEGF, and NO levels were measured. All measurements were done at baseline and after 8 weeks of intervention.Results: A significant weight reduction was observed in all four groups compared to baseline (p=<0.01). The placebo group had significantly higher weight, BMI, weight circumstance (WC), and body fat (BF) compared with the other groups. The α-LA+Faradic group had significantly lower weight, BMI, BF, WC than control, faradic, and α-LA groups and higher, Sitruin,, and PGC than the control group (all P <0.05).Conclusions: The findings indicated that the α-LA and Faradic interventions may have a synergistic effect on weight, BMI, BF, WC, and SLM, possibly through changes in serum level of VEGF, NO, and PGC. Further researches are warranted to clarify the mutual effects of –α-LA and Faradic on obesity and molecular biomarkers.Name of the registry: Iranian Registry of Clinical TrialsTrial registration number: IRCT20131117015424N2Date of registration: https://www.irct.ir/search/result?query=IRCT20131117015424N2URL of trial registry record: 04/04/2018

scholarly journals Two forms of aspartate aminotransferase in rat liver and kidney mitochondria

1968 ◽  
Vol 108 (4) ◽  
pp. 619-624 ◽  
Author(s):  
M. M. Bhargava ◽  
A. Sreenivasan
Keyword(s):  
Rat Liver ◽  
Aspartate Aminotransferase ◽  
High Speed ◽  
Rat Kidney ◽  
Liver Mitochondria ◽  
Supernatant Fraction ◽  
Rat Liver Mitochondria ◽  
Aminotransferase Activity ◽  
Kidney Mitochondria ◽  
Liver And Kidney

1. Butan-1-ol solubilizes that portion of rat liver mitochondrial aspartate aminotransferase (EC 2.6.1.1) that cannot be solubilized by ultrasonics and other treatments. 2. A difference in electrophoretic mobilities, chromatographic behaviour and solubility characteristics between the enzymes solubilized by ultrasonic treatment and by butan-1-ol was observed, suggesting the occurrence of two forms of this enzyme in rat liver mitochondria. 3. Half the aspartate aminotransferase activity of rat kidney homogenate was present in a high-speed supernatant fraction, the remainder being in the mitochondria. 4. A considerable increase in aspartate aminotransferase activity was observed when kidney mitochondrial suspensions were treated with ultrasonics or detergents. 5. All the activity after maximum activation was recoverable in the supernatant after centrifugation at 105000g for 1hr. 6. The electrophoretic mobility of the kidney mitochondrial enzyme was cathodic and that of the supernatant enzyme anodic. 7. Cortisone administration increased the activities of both mitochondrial and supernatant aspartate aminotransferases of liver, but only that of the supernatant enzyme of kidney.

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