scholarly journals Skin-gut axis: The relationship between intestinal bacteria and skin health

2017 ◽  
Vol 6 (4) ◽  
pp. 52-58 ◽  
Author(s):  
Alexandra R Vaughn ◽  
Manisha Notay ◽  
Ashley K Clark ◽  
Raja K Sivamani
Keyword(s):  
Intestinal Bacteria ◽  
The Relationship

PCR-DGGE Analysis of Bacterial Diversity of Intestinal System in Hyperlipidemia Rats

2013 ◽  
Vol 726-731 ◽  
pp. 898-901
Author(s):  
Ri Na Wu ◽  
Xiao Meng Pang ◽  
Xi Yan Wang ◽  
Jun Rui Wu
Keyword(s):  
Bacterial Diversity ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Intestinal Flora ◽  
Intestinal Bacteria ◽  
Control Group ◽  
Rrna Gene ◽  
Dgge Analysis ◽  
Gradient Gel Electrophoresis ◽  
The Relationship ◽  
Insight Into

Denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA gene has been regarded as one of powerful tools for gaining insight into the bacterial diversity of intestinal system. In the present study, hyperlipidemia model was constructed in rat according to the tests of blood lipids. Fecal samples of rats were collected after 60d feeding, and DGGE was used to investigate the diversities of intestinal bacteria in the artificially-induced hyperlipidemia rats and normal rats. The results showed that two patterns had similarities, but there were also some different bacteria communities. Moreover, control group had much more bands than model group on gel, showing species in intestinal of model rats might be deduced by hyperlipidemia. It will be helpful to explore the relationship between hyperlipidemia and intestinal flora.

scholarly journals Effect of intestinal flora on the formation of endometritis of sow

2020 ◽  
Author(s):  
Ling Zhang ◽  
Linkang Wang ◽  
Yimin Dai ◽  
Tianyu Tao ◽  
Jingqi Wang ◽  
...  
Keyword(s):  
Relative Abundance ◽  
Intestinal Flora ◽  
Intestinal Bacteria ◽  
Experimental Result ◽  
Intestinal Microbes ◽  
Birth Canal ◽  
Significant Difference ◽  
Vaginal Secretions ◽  
Fresh Feces ◽  
The Relationship

Abstract Background Aberration of birth canal microbiota is one of the most important factors in the etiology of sow endomentritis. Nevertheless, reports about the structure and composition of birth canal microbiota in endomentritis sow and their relationship with intestinal microbes is limited. Therefore, understanding the relationship between birth canal microbiota and intestinal microbiota of the host has become exceedingly crucial. Results In this study, 4 healthy and 4 endomentritis sows were selected basing on whether the sow had endometritis or not in a farm of China. The microflora of their birth canal secretions and fresh feces were analyzed via sequencing the V3 + V4 region of bacterial 16S rDNA gene. The results showed that the significant difference between endometritis and healthy sows birth canal flora in the composition and abundance. Sow endomentritis was associated with increasing in the relative abundance of Porphyromonas, Clostridium_sensu_stricto_1, Streptococcus, Ezakiella, Fusobacterium, Actinobacillus, Bacteroides, and Prevotella as well as Anaerococcus. On the contrary, the majority of beneficial bacteria that belonging to Firmicutes phylum (e.g., Lactobacillus and Enterococcus ) declined in endomentritis sow. The increased relative abundance of Porphyromonas in the vaginal secretions might correlate with the decrease of Lactobacillusin the feces of endometritis sows. Moreover, the experimental result also found that some intestinal bacteria (such as Escherichia-Shigella and Bacteroides) may be bound up with the onset of sow endometritis. Conclusion Sow endometritis is closely related to the microbiota of birth canal, and that some intestinal bacteria may promote the onset of endometritis. The above results can supply a theoretical basis to research the pathogenesis of endometritis and the microbiota of sow's birth canal and gut.

scholarly journals Association between intestinal bacterial carriage, biomarkers of environmental enteric dysfunction and stunting in rural Malawian children

2021 ◽  
Author(s):  
David Chaima ◽  
John D Hart ◽  
Harry Pickering ◽  
Sarah Burr ◽  
Kenneth Maleta ◽  
...  
Keyword(s):  
Regression Models ◽  
Bifidobacterium Longum ◽  
Intestinal Bacteria ◽  
Cross Sectional Survey ◽  
Cross Sectional ◽  
Environmental Enteric Dysfunction ◽  
Alpha 1 Antitrypsin ◽  
Fecal Biomarkers ◽  
The Relationship ◽  
Height For Age

BackgroundGut bacteria Bifidobacterium longum, Faecalibacterium prausnitzii, Dorea formicigenerans and Akkermansia muciniphila have been implicated in mediation of growth. We investigate the prevalence of these four species, levels of fecal biomarkers of environmental enteric dysfunction (EED) and association with stunting in rural Malawian children. Methods DNA and protein were extracted from fecal samples of 613 children (aged 1-59 months) at a baseline cross-sectional survey in the Mangochi district of Malawi conducted within the framework of the MORDOR (Macrolides Oraux pour Réduire les Décès avec un Oeil sur la Résistance) trial. Intestinal carriage of bacteria was measured by PCR. Neopterin, myeloperoxidase and alpha-1 antitrypsin, biomarkers of EED, were measured by ELISA. Height-for-age Z (HAZ) score <-2 defined stunting. Tests of proportions and regression models were used to explore the relationship between bacterial carriage, EED and stunting.Results B. longum carriage in younger children was associated with elevated EED biomarkers. Two thirds of children had elevated NEO, 33% elevated MPO and 16% elevated AAT. Stunting was found in 38% of the children. No significant associations were found between EED biomarkers or intestinal bacteria carriage and stunting.

scholarly journals Xenobiotics Formed during Food Processing: Their Relation with the Intestinal Microbiota and Colorectal Cancer

2019 ◽  
Vol 20 (8) ◽  
pp. 2051 ◽  
Author(s):  
Alicja M. Nogacka ◽  
María Gómez-Martín ◽  
Adolfo Suárez ◽  
Oscar González-Bernardo ◽  
Clara G. de los Reyes-Gavilán ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Gut Microbiota ◽  
Food Processing ◽  
Dietary Habits ◽  
Scientific Evidence ◽  
Intestinal Bacteria ◽  
Polycyclic Aromatic ◽  
Modifiable Factor ◽  
Microbial Groups ◽  
The Relationship

The colonic epithelium is exposed to a mixture of compounds through diet, among which some are procarcinogens, whereas others have a protective effect. Therefore, the net impact of these compounds on human health depends on the overall balance between all factors involved. Strong scientific evidence has demonstrated the relationship between nitrosamines (NA), heterocyclic amines (HCAs), and polycyclic aromatic hydrocarbons (PAHs), which are the major genotoxins derived from cooking and food processing, and cancer. The mechanisms of the relationship between dietary toxic xenobiotics and cancer risk are not yet well understood, but it has been suggested that differences in dietary habits affect the colonic environment by increasing or decreasing the exposure to mutagens directly and indirectly through changes in the composition and activity of the gut microbiota. Several changes in the proportions of specific microbial groups have been proposed as risk factors for the development of neoplastic lesions and the enrichment of enterotoxigenic microbial strains in stool. In addition, changes in the gut microbiota composition and activity promoted by diet may modify the faecal genotoxicity/cytotoxicity, which can be associated with a higher or lower risk of developing cancer. Therefore, the interaction between dietary components and intestinal bacteria may be a modifiable factor for the development of colorectal cancer in humans and deserves more attention in the near future.

scholarly journals P0761STUDY ON THE MECHANISM OF MICROINFLAMMATION WITH UREMIA CAUSED BY LACTOBACILLUS ACTIVATION OF INTESTINAL MACROPHAGES BY MINCLE

2020 ◽  
Vol 35 (Supplement_3) ◽  
Author(s):  
Lingshuang Sun
Keyword(s):  
Protein Expression ◽  
Sham Group ◽  
Intestinal Bacteria ◽  
Diabetic Patients ◽  
Sprague Dawley ◽  
Transmission Electron ◽  
Sprague Dawley Rats ◽  
Mrna And Protein Expression ◽  
End Stage ◽  
The Relationship

Abstract Background and Aims My previous studies have found that Intestinal macrophages in the uremic rats are polarized towards a proinflammatory phenotype and had dysfunction of phagocytosis leading to aggravate microinflammation and assist bacterial translocation in uremia resulting in microinflammation. However, it is still unclear what kind of mechanism of action of intestinal bacteria activates intestinal macrophages and thus participates in the occurrence and development of microinflammation in uremia. Method Male Sprague-Dawley rats were randomly divided into two groups: sham, uremia. The macrophage ultrastructure was examined by transmission electron microscopy. Immunochemistry was used to analyze the expression of macrophage-inducible C-type lectin (Mincle). RT-PCR and western blot were employed to assess the mRNA and protein expression of toll-like receptor 4 (TLR4). Results Our RCT study found that the number of Lactobacilli in the intestines of patients with end-stage diabetic nephropathy was significantly higher than that in non-diabetic patients(Figure 1). The plasma levels of endotoxin, CRP, IL-6, and TNF-a in the uremia group were greater than those in the sham group (p>0.05)(Table 1).Compared with the sham group, the uremic macrophages showed fewer cytoplasmic protrusions and pseudopodia(Figure 2) and the uremia group exhibited macrophages with higher staining intensities for Mincle and higher mRNA and protein expression of TLR4(Figure 3-5). Conclusion Studies have shown that Lactobacillus planta can directly activate Mincle. The relationship between Mincle and the activation of intestinal macrophages was verified. The solution to this scientific problem will not only clarify the molecular mechanism of intestinal bacteria in controlling the activation of intestinal macrophages, but also link the immune regulation of intestinal macrophages with the micro-inflammation of uremia so as to clarify the micro-inflammation state of uremia.

scholarly journals A One Health Review of Community-Acquired Antimicrobial-Resistant Escherichia coli in India

2021 ◽  
Vol 18 (22) ◽  
pp. 12089
Author(s):  
Keerthana Rajagopal ◽  
Sujith J. Chandy ◽  
Jay P. Graham
Keyword(s):  
Escherichia Coli ◽  
One Health ◽  
Intestinal Bacteria ◽  
One Dimension ◽  
E Coli ◽  
The One ◽  
Relationship Of ◽  
The Relationship ◽  
Third Generation Cephalosporins ◽  
Health Review

Antimicrobial resistance (AMR) threatens to undermine nearly a century of progress since the first use of antimicrobial compounds. There is an increasing recognition of the links between antimicrobial use and AMR in humans, animals, and the environment (i.e., One Health) and the spread of AMR between these domains and around the globe. This systematic review applies a One Health approach—including humans, animals, and the environment—to characterize AMR in Escherichia coli in India. E. coli is an ideal species because it is readily shared between humans and animals, its transmission can be tracked more easily than anaerobes, it can survive and grow outside of the host environment, and it can mobilize AMR genes more easily than other intestinal bacteria. This review synthesized evidence from 38 studies examining antimicrobial-resistant E. coli (AR-E) across India. Studies of AR-E came from 18 states, isolated from different sample sources: Humans (n = 7), animals (n = 7), the environment (n = 20), and combinations of these categories, defined as interdisciplinary (n = 4). Several studies measured the prevalence of AMR in relation to last-line antimicrobials, including carbapenems (n = 11), third-generation cephalosporins (n = 18), and colistin (n = 4). Most studies included only one dimension of the One Health framework, highlighting the need for more studies that aim to characterize the relationship of AMR across different reservoirs of E. coli.

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