Dual Culture Inoculation Enhanced Quality of Silage Produced from Leguminous Plants

2021 ◽  
Vol 41 (3) ◽  
pp. 176-182
Author(s):  
Karnan Muthusamy ◽  
Soundharrajan Ilavenil ◽  
Jeong Sung Jung ◽  
Bae Hun Lee ◽  
Hyung Soo Park ◽  
...  
Keyword(s):  
Dual Culture ◽  
Leguminous Plants

scholarly journals Researches on the separation of impurities from a seed flow based on the aerodynamic principle

2019 ◽  
Vol 112 ◽  
pp. 03011
Author(s):  
Gheorghe Stroescu ◽  
Anişoara Păun ◽  
Gheorghe Voicu ◽  
Carmen Brăcăcescu ◽  
Radu Popa ◽  
...  
Keyword(s):  
Foreign Bodies ◽  
Air Flow ◽  
Practical Importance ◽  
Seed Mass ◽  
Scientific Basis ◽  
Leguminous Plant ◽  
Leguminous Plants ◽  
Plant Seed ◽  
Air Passage

Ensuring for production the necessary quantities of biological material of valuable varieties and hybrids to maintain their initial characteristics requires the permanent application of measures to be carried out on a scientific basis, depending on the biological particularities of the variety or hybrid. The production of seeds for leguminous plants is of great importance because the vast majority of species propagate that way. Due to the fact the yield obtained depends on the quality of the seeds, it is necessary to use seeds of varieties and hybrids with superior qualities and from higher biological categories without foreign bodies (impurities). Knowing the particularities of leguminous plant seed is of special practical importance because the conditioning technologies and machinery are based on it and also the culture technologies that are applied both in the field and in protected areas. The paper presents some theoretical aspects regarding the separation of seed mixtures based on the aerodynamic principle (resistance of the seed mass layer to the air passage, seed floating capacity, air flow working rates, total air flow, physical characteristics of seeds, etc.) which were the basis for designing a conditioning module for vegetable, flower and cereal seeds.

Bioefficacy of rhizospheric fungal isolates against wilt and root-rot pathogens of Pea

2019 ◽  
Vol 5 (01) ◽  
pp. 133-137
Author(s):  
Rashmi S. Nigam ◽  
R. U. Khan ◽  
Reshu Singh ◽  
Joginder Singh
Keyword(s):  
Root Rot ◽  
Pythium Ultimum ◽  
Culture Technique ◽  
Dual Culture ◽  
Yield And Quality ◽  
Fungal Isolates ◽  
Antagonistic Potential ◽  
Production Areas

Pea (Pisum sativum) is an important leguminous crop in many countries including India. Wilt and root rot of pea is an important and widespread disease that often causes significant reduction in the yield and quality of harvested peas throughout the production areas. It is the most important and widespread disease of pea grown in relatively dry and warm area. In-vitro effectiveness of various antagonistic fungal isolates namely T. harzianum (Th1, Th2, Th3, Th4 and Th5) was evaluated against Fusarium oxysporum f. sp. pisi, Rhizoctonia solani and Pythium ultimum by dual culture technique on potato dextrose agar. According to the observation recorded after 5 days, all the rhizospheric fungal isolates evaluated for their antagonistic potential against wilt and root-rot pathogens, exhibited significant effect on radial growth inhibition of pathogens in comparison to control. Among the fungal isolates, Th3 and Th5 of T. harzianum proved to be most effective in reducing the growth of F. oxysporum f. sp. pisi, R. solani and P. ultimum. It was worthy to note that all rhizospheric fungal isolates visualized an increase in their antagonistic potential over the period of time in subsequent hours of inoculation.

Protein quality of leguminous plants determined by proton-induced radiation analysis

1977 ◽  
Vol 142 (1-2) ◽  
pp. 209-211 ◽  
Author(s):  
P. Rupnik ◽  
P. Kump ◽  
M. Budnar ◽  
I. Kreft
Keyword(s):  
Protein Quality ◽  
Leguminous Plants

scholarly journals Bio-Control Efficiency of Trichoderma viride against Stem Rot of Tuberose Caused by Sclerotium rolfsii

2021 ◽  
Vol 10 (11) ◽  
pp. 407-415
Author(s):  
N. H. Sankara Reddy T. Siva Kumar
Keyword(s):  
Growth Parameters ◽  
Trichoderma Viride ◽  
Sclerotium Rolfsii ◽  
Culture Technique ◽  
Raw Material ◽  
Stem Rot ◽  
Dual Culture ◽  
Solid Media ◽  
Poison Food Technique

Tuberose is an important flower crop for cut flower and decorations which is a chief raw material for the production of high-grade perfumes. Recent days stem rot of tuberos is an important disease that affects the quality of flowers. The objective of this paper is to evaluate the efficiency of T. viride isolates against S. rolfsii and improves growth parameters of tuberose. Among the different antagonists tested, Trichoderma is found be very effective against S. rolfsii. The isolates T. viride are named as Tv1 – Tv10. The efficient isolate have been identified as Tv5 by dual culture technique, which shows maximum mycelial inhibition (77.15), this was followed by the isolates Tv4 (75.94) and Tv1 (72.96) in the decreasing order, the least growth inhibition of pathogen was exhibited by the isolate Tv10 (55.72 %). The poison food technique @ 40 % concentration completely inhibits the mycelial growth on solid media.

Immunoferritin localization of intracellular antigens in positively stained frozen sections

1978 ◽  
Vol 36 (2) ◽  
pp. 168-169
Author(s):  
K. T. Tokuyasu
Keyword(s):  
Surface Tension ◽  
Water Surface ◽  
Thin Layers ◽  
The Other ◽  
Positive Staining ◽  
Frozen Sections ◽  
The Past ◽  
Ultrathin Frozen Sections ◽  
Definition Of

During the past investigations of immunoferritin localization of intracellular antigens in ultrathin frozen sections, we found that the degree of negative staining required to delineate u1trastructural details was often too dense for the recognition of ferritin particles. The quality of positive staining of ultrathin frozen sections, on the other hand, has generally been far inferior to that attainable in conventional plastic embedded sections, particularly in the definition of membranes. As we discussed before, a main cause of this difficulty seemed to be the vulnerability of frozen sections to the damaging effects of air-water surface tension at the time of drying of the sections.Indeed, we found that the quality of positive staining is greatly improved when positively stained frozen sections are protected against the effects of surface tension by embedding them in thin layers of mechanically stable materials at the time of drying (unpublished).

Lithography below 100 nm

1983 ◽  
Vol 41 ◽  
pp. 96-99
Author(s):  
L. D. Jackel
Keyword(s):  
Spatial Distribution ◽  
Electron Beam ◽  
Electron Scattering ◽  
Electron Beam Lithography ◽  
Substrate Material ◽  
Local Electron ◽  
Electron Dose ◽  
Positive Resist ◽  
Exposure Process

Most production electron beam lithography systems can pattern minimum features a few tenths of a micron across. Linewidth in these systems is usually limited by the quality of the exposing beam and by electron scattering in the resist and substrate. By using a smaller spot along with exposure techniques that minimize scattering and its effects, laboratory e-beam lithography systems can now make features hundredths of a micron wide on standard substrate material. This talk will outline sane of these high- resolution e-beam lithography techniques.We first consider parameters of the exposure process that limit resolution in organic resists. For concreteness suppose that we have a “positive” resist in which exposing electrons break bonds in the resist molecules thus increasing the exposed resist's solubility in a developer. Ihe attainable resolution is obviously limited by the overall width of the exposing beam, but the spatial distribution of the beam intensity, the beam “profile” , also contributes to the resolution. Depending on the local electron dose, more or less resist bonds are broken resulting in slower or faster dissolution in the developer.

Convergent Beam Electron Diffraction

1978 ◽  
Vol 36 (3) ◽  
pp. 304-315
Author(s):  
G. Lehmpfuhl
Keyword(s):  
Electron Diffraction ◽  
Diffraction Pattern ◽  
Crystal Surface ◽  
Diffraction Spot ◽  
Crystal Thickness ◽  
Large Area ◽  
Electron Microscopic ◽  
Additional Information ◽  
Microscopic Investigations

Introduction In electron microscopic investigations of crystalline specimens the direct observation of the electron diffraction pattern gives additional information about the specimen. The quality of this information depends on the quality of the crystals or the crystal area contributing to the diffraction pattern. By selected area diffraction in a conventional electron microscope, specimen areas as small as 1 µ in diameter can be investigated. It is well known that crystal areas of that size which must be thin enough (in the order of 1000 Å) for electron microscopic investigations are normally somewhat distorted by bending, or they are not homogeneous. Furthermore, the crystal surface is not well defined over such a large area. These are facts which cause reduction of information in the diffraction pattern. The intensity of a diffraction spot, for example, depends on the crystal thickness. If the thickness is not uniform over the investigated area, one observes an averaged intensity, so that the intensity distribution in the diffraction pattern cannot be used for an analysis unless additional information is available.

Observability of Very Thick Specimen by Using Transmission Scanning Electron Microscope

1971 ◽  
Vol 29 ◽  
pp. 26-27
Author(s):  
K. Shibatomi ◽  
T. Yamanoto ◽  
H. Koike
Keyword(s):  
Electron Microscope ◽  
Image Quality ◽  
Scanning Electron Microscope ◽  
Chromatic Aberration ◽  
Image Contrast ◽  
Objective Lens ◽  
Thick Specimen ◽  
Transmission Electron ◽  
Scanning Electron

In the observation of a thick specimen by means of a transmission electron microscope, the intensity of electrons passing through the objective lens aperture is greatly reduced. So that the image is almost invisible. In addition to this fact, it have been reported that a chromatic aberration causes the deterioration of the image contrast rather than that of the resolution. The scanning electron microscope is, however, capable of electrically amplifying the signal of the decreasing intensity, and also free from a chromatic aberration so that the deterioration of the image contrast due to the aberration can be prevented. The electrical improvement of the image quality can be carried out by using the fascionating features of the SEM, that is, the amplification of a weak in-put signal forming the image and the descriminating action of the heigh level signal of the background. This paper reports some of the experimental results about the thickness dependence of the observability and quality of the image in the case of the transmission SEM.

The Current Situation in Chemical Stabilization of Biological Materials

1972 ◽  
Vol 30 ◽  
pp. 132-133
Author(s):  
John H. Luft
Keyword(s):  
Electron Microscopy ◽  
Electron Microscope ◽  
Osmium Tetroxide ◽  
Molecular Level ◽  
Cross Linking ◽  
Chemical Stabilization ◽  
Specimen Preparation ◽  
Sufficient Condition ◽  
Radio Telescopes

With information processing devices such as radio telescopes, microscopes or hi-fi systems, the quality of the output often is limited by distortion or noise introduced at the input stage of the device. This analogy can be extended usefully to specimen preparation for the electron microscope; fixation, which initiates the processing sequence, is the single most important step and, unfortunately, is the least well understood. Although there is an abundance of fixation mixtures recommended in the light microscopy literature, osmium tetroxide and glutaraldehyde are favored for electron microscopy. These fixatives react vigorously with proteins at the molecular level. There is clear evidence for the cross-linking of proteins both by osmium tetroxide and glutaraldehyde and cross-linking may be a necessary if not sufficient condition to define fixatives as a class.

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