scholarly journals PHYTOCHEMICAL ANALYSIS AND ANTIOXIDANT ACTIVITY OF THE HYDROETHANOLIC EXTRACT OF Passiflora edulis f. flavicarpa RESIDUES

Author(s):  
Jussimara Barros De Oliveira ◽  
Jorge Vitório Gomes Das Neves ◽  
Marcondes Viana Da Silva
Foods ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 142
Author(s):  
Aikaterini Koutsaviti ◽  
Samer Toutoungy ◽  
Rouba Saliba ◽  
Sofia Loupassaki ◽  
Olga Tzakou ◽  
...  

The antioxidant activity of the essential oils, as well as of the organic and hydroethanolic extracts, of the fresh needles of 54 pine taxa was evaluated using the peroxy-oxalate and luminol chemiluminescence assays. Among all evaluated essential oils, P. canariensis and P. attenuata displayed the highest levels of activity. P. contorta var. murrayana, followed by P. nigra var. caramanica, exhibited the highest antioxidant capacity among the organic extracts, while the most active hydroethanolic extract was that of P. nigra subsp. nigra. Based on the overall levels of activity, the latter taxon was selected for phytochemical analysis targeting the isolation of the bioactive constituents. As such, the organic extract of P. nigra subsp. nigra was subjected to chromatographic separations to yield 11 secondary metabolites (1−11) that were evaluated for their antioxidant activity. Nonetheless, the isolated compounds were found to be less active than the crude extract, thus suggesting the potential role of synergism.


2019 ◽  
Vol 70 (1) ◽  
pp. 23-26
Author(s):  
Sebastian Mihai ◽  
Denisa Dumitrescu ◽  
Madalina Ana Raducanu ◽  
Iuliana Stoicescu ◽  
Victoria Badea

The scope of this work is a phytochemical analysis and antioxidant activity assay of a Sempervivum ruthenicum Koch hydroethanolic extract. The hydroethanolic extract was prepared from the dried leaves of the plant by maceration in a water and methanol mixture (50:50 v/v). The total phenolic content of the extract was calculated to be 3.0501�0.0272 mg/mL and the total flavonoid content was determined in a concentration of 3.113�0.0394 mg/mL. The HPLC-DAD analysis revealed phenolic acids and flavonoids, which were quantified. The most prevalent phenolic acids in the extracts were gallic and ellagic acids, with concentrations of 1.2443�0.0475 mg/mL, respectively 0.6339�0.0026 mg/mL. The heteroside astragalin was present in high concentration of 1.1934�0.0754 mg/mL. The DPPH free radical scavenging assay revealed the EC50 value of the extract to be 2.5788�0.003 mg/mL. These results suggest a good scavenging ability of the extract, which is due to the abundance of polyphenolic compounds. The antioxidant activity of the extract demonstrates a high scavenging ability at low doses. Sempervivum ruthenicum Koch shows a promising phytochemical profile that suggests it�s use in pathological conditions that involve high oxidative stress.


2018 ◽  
Author(s):  
Bárbara Cabral ◽  
Jovelina Samara Alves ◽  
Tays Amanda Gonçalves ◽  
Priscilla Maria Maciel ◽  
Isac Medeiros ◽  
...  

F1000Research ◽  
2018 ◽  
Vol 7 ◽  
pp. 1839
Author(s):  
Harlinda Kuspradini ◽  
Indah Wulandari ◽  
Agmi Sinta Putri ◽  
Sabeti Yulis Tiya ◽  
Irawan Wijaya Kusuma

Background: Litsea angulata is a plant species belonging to Lauraceae family that is distributed throughout Indonesia, Malaysia, and New Guinea. The seeds have been traditionally used by local people in Kalimantan, Indonesia for the treatment of boils; however, there is no information about the potency of its branch, bark and leaves yet. This study aimed to determine the antioxidant, antimicrobial activity as well as the phytochemical constituent of Litsea angulata branch, bark, and leaves. Methods: Extraction was performed by successive maceration method using n-hexane, ethyl acetate, and ethanol solvent. Antioxidant activity was evaluated by DPPH radical scavenging assay. The antimicrobial activity using the 96 well-plate microdilution broth method against Staphylococcus aureus and Streptococcus mutans. Results: Based on the phytochemical analysis, it showed that extract of L. angulata contains alkaloids, flavonoids, tannins, terpenoids, and coumarin. The results showed that all extracts of plant samples displayed the ability to inhibit DPPH free radical formation and all tested microorganisms. Conclusions: L. angulata contains secondary metabolites such as alkaloids, flavonoids, tannins, terpenoids, carotenoids, and coumarin. The antioxidant activity on different plant extracts was a range as very strong to weak capacity. All extracts in this study could inhibit the growth of S. aureus and S. mutans.


2018 ◽  
Vol 2018 ◽  
pp. 1-12 ◽  
Author(s):  
Andra-Diana Andreicut ◽  
Alina Elena Pârvu ◽  
Augustin Cătălin Mot ◽  
Marcel Pârvu ◽  
Eva Fischer Fodor ◽  
...  

Oxidative stress and inflammation are interlinked processes. The aim of the study was to perform a phytochemical analysis and to evaluate the antioxidant and anti-inflammatory activities of ethanolic Mahonia aquifolium flower (MF), green fruit (MGF), and ripe fruit (MRF) extracts. Plant extract chemical composition was evaluated by HLPC. A DPPH test was used for the in vitro antioxidant activity. The in vivo antioxidant effects and the anti-inflammatory potential were tested on a rat turpentine oil-induced inflammation, by measuring serum nitric oxide (NOx) and TNF-alpha, total oxidative status (TOS), total antioxidant reactivity (TAR), oxidative stress index (OSI), 3-nitrothyrosine (3NT), malondialdehyde (MDA), and total thiols (SH). Extracts were administrated orally in three dilutions (100%, 50%, and 25%) for seven days prior to inflammation. The effects were compared to diclofenac. The HPLC polyphenol and alkaloid analysis revealed chlorogenic acid as the most abundant compound. All extracts had a good in vitro antioxidant activity, decreased NOx, TOS, and 3NT, and increased SH. TNF-alpha was reduced, and TAR increased only by MF and MGF. MDA was not influenced. Our findings suggest that M. aquifolium has anti-inflammatory and antioxidant effects that support the use in primary prevention of the inflammatory processes.


2017 ◽  
Vol 9 (5) ◽  
pp. 116 ◽  
Author(s):  
Khoushika Raajshree R. ◽  
Brindha Durairaj

Objective: The present study was designed to evaluate the antioxidant and antityrosinase properties of zinc oxide nanoparticles (ZnO-NPs) synthesized from brown seaweed Turbinaria conoides.Methods: Zinc Oxide Nanoparticles were synthesized from the hydroethanolic extract of Turbinaria conoides. Ultraviolet-Visible Spectrophotometric analysis was performed to confirm the formation of ZnO-NPs. Size, morphology and elemental composition of ZnO-NPs were analysed using SEM-EDAX. The antioxidant activity of the synthesized zinc oxide nanoparticles was investigated by total antioxidant capacity (phosphomolybdenum method), reducing power assay and ferric reducing antioxidant power assay (FRAP). Anti tyrosinase activity was assessed to validate the skin whitening ability of the ZnO-NPs. Results: The antioxidant activity of ZnO-NPs synthesized from hydroethanolic extract of Turbinaria conoides was maximum when compared with that of the hydroethanolic algal extract. The antityrosinase activity of ZnO-NPs was found to be maximum with 75% tyrosinase inhibition when compared to hydroethanolic algal extract which had 56% inhibition at 250μg/ml concentration.Conclusion: Overall our study provides a firm evidence to support that antityrosinase and antioxidant activities are exhibited by ZnO-NPs synthesized from hydroethanolic extract of Turbinaria conoides and it might be used as an antioxidant and as a source of skin whitening agent in cosmetics.


2018 ◽  
Vol 8 (4) ◽  
Author(s):  
Nitish A Bharadwaj ◽  
K.K. Karthik Udupa ◽  
S Karthik ◽  
K.S. Vinayaka ◽  
T.R. Prashith Kekuda

Author(s):  
Mukesh S Sikarwar ◽  
Chew Khai Szeek ◽  
Neeraj Paliwal

Background: Herbal medicine mostly contains wide range of chemical compounds responsible for medicinal therapeutic use. Costus woodsonii is commonly called as Red Button Ginger and synonyms of the botanical name are Costus spiralis, Alpinia spiralis and Costus pisonis. In Malay, it is known as Setawar Halia Merah. In Chinese, it is known as Hong Bi Qiao Jiang. Objective: This research was conducted to study the pharmacognostical, phytochemical, antioxidant and antimicrobial activity of C. woodsonii leaf extracts. Method: Macroscopy, microscopy, phytochemical analysis, thin layer chromatography, antioxidant activity and antimicrobial activity of C. woodsonii leaf were carried out. Total flavonoids were estimated in the leaf extract. The total phenolic content of C. woodsonii leaf was determined using Folin-Ciocalteu reagent. The antioxidant activity of leaf extract of C. woodsonii was determined by performing DPPH radical scavenging. The microbial activity was determined by Well diffusion test, MIC (Minimum Inhibitory Concentration) test and MBC (Minimum Bactericidal Concentration) test. Result and Discussion: C. woodsonii belongs to costaceae with elliptical green leaves. Till now are no extensive studies on C. woodsonii. Preliminary phytochemical analysis revealed the presence of flavonoid, steroid, fat, phenol, tannin and mucilage in leaf extract. Physicochemical studies further revealed the ash value of leaf as 8.7%. Among the three extractions, alcohol extractive values showed the highest as 13%. Loss on drying at 105 degree Celsius in leaf was found to be 12.67%. The plant extract showed total phenolic content of 7.941 mg GAE/g at concentration of 5µg/ml. As for flavonoids content, plant extract showed 21.7 mg RE/g at concentration of 200µg/ml and 43.4 mg RE/g at concentration of 400µg/ml. For antioxidant activity, the plant extract showed weak antioxidant activity in DPPH scavenging activity assay. For antimicrobial test, the leaf extract of C. woodsonii showed weak antimicrobial activity. Conclusion: From this study, it can conclude that C. woodsonii leaf extract possess weak antioxidant activity and weak antimicrobial activity which need to be further validated by using more antioxidant assays and antimicrobial tests.


Sign in / Sign up

Export Citation Format

Share Document