scholarly journals Antioxidant Potential of Pine Needles: A Systematic Study on the Essential Oils and Extracts of 46 Species of the Genus Pinus

Foods ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 142
Author(s):  
Aikaterini Koutsaviti ◽  
Samer Toutoungy ◽  
Rouba Saliba ◽  
Sofia Loupassaki ◽  
Olga Tzakou ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Essential Oils ◽  
Phytochemical Analysis ◽  
Bioactive Constituents ◽  
Chromatographic Separations ◽  
Organic Extract ◽  
Luminol Chemiluminescence ◽  
Organic Extracts ◽  
Hydroethanolic Extract

The antioxidant activity of the essential oils, as well as of the organic and hydroethanolic extracts, of the fresh needles of 54 pine taxa was evaluated using the peroxy-oxalate and luminol chemiluminescence assays. Among all evaluated essential oils, P. canariensis and P. attenuata displayed the highest levels of activity. P. contorta var. murrayana, followed by P. nigra var. caramanica, exhibited the highest antioxidant capacity among the organic extracts, while the most active hydroethanolic extract was that of P. nigra subsp. nigra. Based on the overall levels of activity, the latter taxon was selected for phytochemical analysis targeting the isolation of the bioactive constituents. As such, the organic extract of P. nigra subsp. nigra was subjected to chromatographic separations to yield 11 secondary metabolites (1−11) that were evaluated for their antioxidant activity. Nonetheless, the isolated compounds were found to be less active than the crude extract, thus suggesting the potential role of synergism.

scholarly journals Habitat Affects the Chemical Profile, Allelopathy, and Antioxidant Properties of Essential Oils and Phenolic Enriched Extracts of the Invasive Plant Heliotropium Curassavicum

Plants ◽  
2019 ◽  
Vol 8 (11) ◽  
pp. 482 ◽  
Author(s):  
Ahmed M. Abd-ElGawad ◽  
Abdelsamed I. Elshamy ◽  
Saud L. Al-Rowaily ◽  
Yasser A. El-Amier
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Essential Oils ◽  
Invasive Plant ◽  
Biological Activities ◽  
Antioxidant Properties ◽  
Bioactive Constituents ◽  
Chenopodium Murale ◽  
Two Samples ◽  
Ic50 Values

The variation in habitat has a direct effect on the plants and as a consequence, changes their content of the bioactive constituents and biological activities. The present study aimed to explore the variation in the essential oils (EOs) and phenolics of Heliotropium curassavicum collected from the coastal and inland habitats. Additionally, we determined their antioxidant and allelopathic activity against the weed, Chenopodium murale. Fifty-six compounds were identified as overall from EOs, from which 25 components were identified from the coastal sample, and 52 from the inland one. Sesquiterpenes were the main class in both samples (81.67% and 79.28%), while mono (3.99% and 7.21%) and diterpenes (2.9% and 1.77%) represented minors, respectively. Hexahydrofarnesyl acetone, (-)-caryophyllene oxide, farnesyl acetone, humulene oxide, farnesyl acetone C, and nerolidol epoxy acetate were identified as major compounds. The HPLC analysis of MeOH extracts of the two samples showed that chlorogenic acid, rutin, and propyl gallate are major compounds in the coastal sample, while vanilin, quercetin, and 4′,7-dihydroxyisoflavone are majors in the inland one. The EOs showed considerable phytotoxicity against C. murale with IC50 value of 2.66, 0.59, and 0.70 mg mL−1 for germination, root, and shoot growth, respectively from the inland sample. While the coastal sample attained the IC50 values of 1.58, 0.45, and 0.66 mg mL−1. MeOH extracts revealed stronger antioxidant activity compared to the EOs. Based on IC50 values, the ascorbic acid revealed 3-fold of the antioxidant compared to the EO of the coastal sample and 4-fold regarding the inland sample. However, the ascorbic acid showed 3-fold of the antioxidant activity of the MeOH extracts of coastal and inland samples. Although H. curassavicum is considered as a noxious, invasive plant, the present study revealed that EO and MeOH extracts of the H. curassavicum could be considered as promising, eco-friendly, natural resources for antioxidants as well as weed control, particularly against the weed, C. murale.

Phytochemical Analysis and Assessment of Biological Properties of Essential Oils Obtained from Thyme and Rosmarinus Species

2020 ◽  
Vol 21 (5) ◽  
pp. 414-424
Author(s):  
Amira Zaïri ◽  
Sahar Nouir ◽  
Mohamed A. Khalifa ◽  
Bouraoui Ouni ◽  
Houda Haddad ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Essential Oils ◽  
Traditional Medicine ◽  
Plant Species ◽  
Antioxidant Activities ◽  
Biological Properties ◽  
Gas Chromatography Mass Spectrometry ◽  
Phytochemical Analysis ◽  
Cytotoxic Potential ◽  
Thymus Algeriensis

Background: The plant species Thymus algeriensis (TA); Thymus capitatus (TC) and Rosmarinus officinalis (RO), are widely used in traditional medicine in Tunisia. The bioactivities of their essential oils have been also reported previously. The main objective of this work was to assess the phytochemical composition, the antioxidant activity, cytotoxic potential and the antibacterial, antifungal, of the essential oil (EO) of these plants. Methods: Gas chromatography–mass spectrometry (GC-MS) was used to identify and quantify the constituents of the tested EO, chemical tests and spectrophotometric methods were used for antioxidant activities and for the screening, the quantification of phytochemicals. The cytotoxic potential of the EO was checked using HCT 116 cultures. Extracts were evaluated for their antibacterial potential by the microdilution method. Antifungal activities were tested using the Poisoned food method against: Aspergillus niger and Aspergillus flavus. Results: The EO of tested plants presented several components, mainly monoterpenes and sesquiterpenes. Results revealed that T. capitatus EO is not toxic comparing to the other tested samples. Phenolic compounds were detected and this EO showed excellent antioxidant activity presenting dose dependent relationship. Regarding antimicrobial activity, T. capitatus EO, also had the highest inhibition against all tested bacteria and fungi. Conclusions: This study showed the importance of the bioactivities (antioxidant, antimicrobial, and safety potential) of EOs of the plant species TC, RO, and TA used in traditional medicine.

scholarly journals Competing Role of Bioactive Constituents inMoringa oleiferaExtract and Conventional Nutrition Feed on the Performance of Cobb 500 Broilers

2015 ◽  
Vol 2015 ◽  
pp. 1-13 ◽  
Author(s):  
Govindarajan Karthivashan ◽  
Palanisamy Arulselvan ◽  
Abd. Razak Alimon ◽  
Intan Safinar Ismail ◽  
Sharida Fakurazi
Keyword(s):  
Antioxidant Activity ◽  
Meat Quality ◽  
Antioxidant Status ◽  
Cost Effective ◽  
Bioactive Constituents ◽  
Broiler Meat ◽  
Antioxidant Parameters ◽  
Antioxidant Effects

The influence ofMoringa oleifera(MO) leaf extract as a dietary supplement on the growth performance and antioxidant parameters was evaluated on broiler meat and the compounds responsible for the corresponding antioxidant activity were identified. 0.5%, 1.0%, and 1.5% w/v of MO leaf aqueous extracts (MOLE) were prepared, and nutritional feed supplemented with 0%, 0.5%, 1.0%, and 1.5% w/w of MO leaf meal (MOLM) extracts were also prepared and analysed for theirin vitroantioxidant potential. Furthermore, the treated broiler groups (control (T1) and treatment (T2, T3, and T4)) were evaluated for performance, meat quality, and antioxidant status. The results of this study revealed that, among the broilers fed MOLM, the broilers fed 0.5% w/w MOLM (T2) exhibited enhanced meat quality and antioxidant status (P<0.05). However, the antioxidant activity of the MOLE is greater than that of the MOLM. The LC-MS/MS analysis of MOLM showed high expression of isoflavones and fatty acids from soy and corn source, which antagonistically inhibit the expression of the flavonoids/phenols in the MO leaves thereby masking its antioxidant effects. Thus, altering the soy and corn gradients in conventional nutrition feed with 0.5% w/w MO leaves supplement would provide an efficient and cost-effective feed supplement.

scholarly journals Phytochemical Analysis and Study of Antioxidant, Anticandidal, and Antibacterial Activities of Teucrium polium subsp. polium and Micromeria graeca (Lamiaceae) Essential Oils from Northern Morocco

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Taoufiq Benali ◽  
Khaoula Habbadi ◽  
Abdelhakim Bouyahya ◽  
Abdelmajid Khabbach ◽  
Ilias Marmouzi ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Essential Oils ◽  
Pathogenic Bacteria ◽  
Reducing Power ◽  
Antimicrobial Effect ◽  
Antibacterial Activities ◽  
Food Preservation ◽  
Phytochemical Analysis ◽  
Teucrium Polium

The protection of agricultural crops and the preservation of the organoleptic and health qualities of food products represent a major challenge for the agricultural and agro-food industries. Essential oils have received greater attention as alternatives to replace the control strategies based on pesticides against phytopathogenic bacteria and synthetic compounds in food preservation. The aims of this work were to study the chemical composition of Teucrium polium subsp. polium and Micromeria graeca essential oils and to examine their antioxidant and antimicrobial effects. To carry out this work, the chemical composition of the essential oil was determined using gas chromatography (GC) with the detection feature of mass spectrometry (MS). Subsequently, the antioxidant activity was investigated by DPPH and FRAPS assays. The antimicrobial effect was studied against phytopathogenic and foodborne pathogenic bacteria using the disc and the microdilution methods. Our results showed that GC-MS analysis of EOs allowed the identification of 30 compounds in T. polium EO (TPpEO), while 5 compounds were identified in M. graeca EO (MGEO). TPpEO had as major compounds β-pinene (19.82%) and germacrene D (18.33%), while geranial (36.93%) and z-citral (18.25%) were the main components of MGEO. The most potent activity was obtained from MGEO (IC50 = 189.7 ± 2.62 µg/mL) compared to TPpEO (IC50 = 208.33 ± 3.51 µg/mL. For the FRAP test, the highest reducing power was obtained from 1.32 ± 0.1 mg AAE/g of TPpEO compared to MGEO 0.51 ± 0.13 mg AAE/g of EO. Both EOs exhibited varying degrees of antibacterial activities against all the tested strains with inhibition zones in the range of 9.33 ± 0.57 mm to >65 mm and MIC values from 0.19 to 12.5 mg/mL. However, MGEO exhibits an interesting anticandidal effect with inhibition zone 44.33 ± 0.57 mm. The findings of this research establish the riches of EOs on volatile compounds, their important antioxidant activity, and their antimicrobial effect against the bacteria tested.

scholarly journals Phytochemical Profile and Total Antioxidant Capacity of Sempervivum ruthenicum Koch Hydroethanolic Extract

2019 ◽  
Vol 70 (1) ◽  
pp. 23-26
Author(s):  
Sebastian Mihai ◽  
Denisa Dumitrescu ◽  
Madalina Ana Raducanu ◽  
Iuliana Stoicescu ◽  
Victoria Badea
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Acids ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
High Concentration ◽  
Total Antioxidant ◽  
Phytochemical Profile ◽  
Free Radical Scavenging Assay ◽  
Hydroethanolic Extract

The scope of this work is a phytochemical analysis and antioxidant activity assay of a Sempervivum ruthenicum Koch hydroethanolic extract. The hydroethanolic extract was prepared from the dried leaves of the plant by maceration in a water and methanol mixture (50:50 v/v). The total phenolic content of the extract was calculated to be 3.0501�0.0272 mg/mL and the total flavonoid content was determined in a concentration of 3.113�0.0394 mg/mL. The HPLC-DAD analysis revealed phenolic acids and flavonoids, which were quantified. The most prevalent phenolic acids in the extracts were gallic and ellagic acids, with concentrations of 1.2443�0.0475 mg/mL, respectively 0.6339�0.0026 mg/mL. The heteroside astragalin was present in high concentration of 1.1934�0.0754 mg/mL. The DPPH free radical scavenging assay revealed the EC50 value of the extract to be 2.5788�0.003 mg/mL. These results suggest a good scavenging ability of the extract, which is due to the abundance of polyphenolic compounds. The antioxidant activity of the extract demonstrates a high scavenging ability at low doses. Sempervivum ruthenicum Koch shows a promising phytochemical profile that suggests it�s use in pathological conditions that involve high oxidative stress.

The chemical composition and antioxidant activity of essential oils and extracts of Dalbergia odorifera leaves

Holzforschung ◽  
2020 ◽  
Vol 74 (8) ◽  
pp. 755-763
Author(s):  
Ruoke Ma ◽  
Heng Liu ◽  
Fujun Shi ◽  
Yunlin Fu ◽  
Penglian Wei ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Antioxidant Activity ◽  
Chemical Composition ◽  
Essential Oils ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Biochanin A ◽  
Gas Chromatography Mass Spectrometry ◽  
Phytochemical Analysis ◽  
Dalbergia Odorifera

AbstractTo analyze the chemical composition and evaluate the potential commercial value of Dalbergia odorifera leaves, the chemical component and content of the essential oils (EOs), fatty acids (FAs), polyphenols and flavonoids in the leaves of D. odorifera were studied. The composition of the EOs obtained by simultaneous distillation extraction (SDE) (at the yield of 0.092%) and hydro-distillation (HD) (at the yield of 0.050%) from D. odorifera leaves was analyzed by gas chromatography-mass spectrometry (GC-MS). Seventy-six compounds were identified and the major compounds were phytol (22.5–24.3%), 4-vinylguaiacol (8.7–12.7%), dihydroedulan II (7.4–11.2%), γ-elemene (5.1–6.2%), cedrol (2.8–6.1%), coumaran (0.9–3.1%) and hexahydrofarnesyl acetone (2.5–2.7%). The FA yield extracted by ultrasound was 1.145% (w/w), and the most abundant FA components were palmitic acid (C16:0), oleic acid (C18:1) and linoleic acid (C18:2) in leaves. The results of the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis (3-ethylbenzothiazoli-ne-6-sulfonic acid (ABTS+) radical scavenging experiments demonstrated that the EOs and FAs possess strong antioxidant activity. The ethanol extract of the leaves had a high polyphenol content [16.00 mg gallic acid equivalent (GAE) g−1 dry weight (DW)] and flavonoid content [8.92 mg rutin equivalent (RE) g−1 DW]. This research has determined qualitatively and quantitatively the isoflavonoids (biochanin A, isoliquiritigenin, tectorigenin and genistein) by ultra-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UPLC-ESI-MS/MS). The phytochemical analysis (EOs, FAs and flavonoids) of the extract showed that the D. odorifera leaf is a rich source of high bioactive compounds and might have the potential to be recommended for further cosmetics, food and pharmaceutical applications.

scholarly journals Phytochemical compounds of Guibourtia ehie and their antioxidant, urease and α-glucosidase inhibitory activities

2022 ◽  
Author(s):  
Laurent Voufack Lefack Bongmo ◽  
Gervais Mouthé Happi ◽  
George Bellier Tabekoueng ◽  
Mehreen Lateef ◽  
Alain François Kamdem Waffo ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Inhibitory Activity ◽  
Stem Bark ◽  
Phytochemical Analysis ◽  
Bioactive Constituents ◽  
Phytochemical Compounds ◽  
Inhibitory Activities

Thirteen compounds (<b>1</b>‒<b>13</b>) were isolated and identified during phytochemical analysis of the leaves and stem bark of <i>Guibourtia ehie</i> (A. Chev) J. Leonard. Spectroscopic and spectrometric methods and the comparison of their results with those given in the literature were used to ascertain their structures. Furthermore, the acetylation of 3,3′-di-<i>O</i>-methylellagic acid 4′-<i>O</i>-β-D-xylopyranoside (<b>2</b>) afforded a new derivative 3,3′-di-<i>O</i>-methylellagic acid 4′-<i>O</i>-β-D-(4,2′′,4′′-triacetyl)-xylopyranoside (<b>2a</b>). Extracts, fractions, and isolated compounds were assessed for their antioxidant, urease, and α-glucosidase inhibitory activities. Compound <b>1</b> demonstrated potent antioxidant activity in the DPPH with an IC<sub>50</sub> value of 36.4 ± 0.2 µM, while rhaponticin (<b>3</b>), 2,6-dimethoxybenzoquinone (<b>4</b>), and taraxerol (<b>6</b>) exhibited a strong α-glucosidase inhibitory activity with the IC<sub>50</sub> values of 35.5 ± 0.1, 25.5 ± 0.2 and 43.4 ± 0.3 µM, respectively. The present study enriches the chemistry of <i>Guiboutia ehie</i> and provides further evidence on its bioactive constituents, which might help in the development of hypoglycaemic drugs.

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