scholarly journals STUDY OF CLINICAL AND BIOCHEMICAL INDICATORS IN MUSCLE PATHOLOGY AMONG PATIENTS WITH HEMOPHILIA

2021 ◽  
Vol 14 (3) ◽  
Author(s):  
O Madasheva ◽  
A Makhmudova ◽  
I Berger
Keyword(s):  
Muscle Tissue ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Cholesterol Content ◽  
Muscle Pathology ◽  
Control Group ◽  
Diagnosis And Prognosis ◽  
Rate Of Progression ◽  
The Republic ◽  
Apparently Healthy

Aim. Determine the state of the biochemical composition of blood in the midst and resolution of muscle hematomas in patients with hemophilia.Materials and methods. 118 patients with hemophilia were examined. These patients were inpatient treatment at the hemophilia center at the Republican Specialized Scientific and Practical Hematology Center of the Ministry of Health of the Republic of Uzbekistan. The control group consisted of 40 apparently healthy men of the same age. Results. Signs of massive hemorrhages in the tissue are an increase in the blood level of the activity of the cytoplasmic enzyme aspartate aminotransferase (54.1 ± 3.9 and 18 ± 4.1, respectively) and alanine aminotransferase (55.3 ± 5.2 and 27.3 ± 4.4, respectively). Depending on the severity of the breakdown of muscle fibers and the rate of progression of the complication, the increase in CPK activity in myopathies can be either sharp - 680.7 ± 14.2 IU / L (hemophilic tumor, tissue necrosis), or, conversely, insignificant 120.3 ± 11.1 IU / L (muscle tissue contracture or atrophy). Deficiency of serum iron (6.4 ± 1.1 μmol / L) was revealed in all groups of examined patients with hemophilia. There was a tendency to lower cholesterol content to 3.3 ± 0.2 mmol / L. The content of urea, as the end product of protein metabolism, was higher in hemophilia patients with a syndrome of necrotic changes in hematomas – in 4 patients (9.8 ± 2.2 mmol / l), which, in our opinion, is associated with an increase in the breakdown of hemoglobin and other proteins with massive hemolysis of erythrocytes in tissues. The study of trace elements in the blood serum revealed a deficiency of Mg²+ 1.6 times, Ca²+ 2.1 times.Conclision. In patients with hemophilia with hemorrhages in muscle tissue, there was a tendency to lower cholesterol and iron levels, and an increase in the amount of bilirubin. The “key” enzymes for the diagnosis and prognosis of complications can be a dynamic study of the activity of the levels of CPK (creatine phosphokinase) and ALT (alanine aminotransferase), AST (aspartate aminotransferase).

scholarly journals Predictive value of the aspartate aminotransferase to platelet ratio index and aspartate aminotransferase to alanine aminotransferase ratio in early diagnosis of intrahepatic cholestasis in pregnancy

2021 ◽  
Vol 8 (11) ◽  
pp. 650-654
Author(s):  
İbrahim Kale
Keyword(s):  
Platelet Count ◽  
Intrahepatic Cholestasis ◽  
Predictive Value ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
First Trimester ◽  
Control Group ◽  
Third Trimester ◽  
Apri Score ◽  
In Pregnancy

Objective: We aimed to investigate the predictive value of the first-trimester aspartate aminotransferase/platelet count ratio index (APRI) and aspartate aminotransferase/alanine aminotransferase ratio for intrahepatic cholestasis in pregnancy (ICP). Material and Methods: The clinical data of patients who admitted to the Obstetrics Department of Umraniye Training and Research Hospital, between 2015-2020 were analyzed retrospectively. The study group consisted of 44 patients with ICP and the control group consisted of randomly selected 92 healthy pregnant women. Results: The two groups were similar in terms of age, BMI, first and third-trimester platelet count and third-trimester hemoglobin level. Patients with ICP had a significantly higher first-trimester APRI and a lower first trimester AST/ALT ratio than the healthy controls (p <0.001, p = 0.001, respectively). According to the ROC analysis, the optimal cut-off value of the APRI to predict ICP was 0.191, with the sensitivity of 0.66 and specificity of 0.66 (AUC: 0,727), and the optimal cut-off value for AST/ALT ratio was 1.07, with the sensitivity of 0.64, and specificity of 0.62 (AUC: 0,681). Conclusion: The first-trimester APRI score and AST/ALT ratio is an easy, inexpensive, and non-invasive tool that may be useful in predicting ICP early.

The effects of ethanol (0.75 g/kg body weight) on the activities of selected enzymes in sera of healthy young adults: 1. Intermediate-term effects.

1977 ◽  
Vol 23 (5) ◽  
pp. 830-834 ◽  
Author(s):  
D E Freer ◽  
B E Statland
Keyword(s):  
Young Adults ◽  
Body Weight ◽  
Enzyme Activity ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Ethanol Ingestion ◽  
Similar Time ◽  
Gamma Glutamyltransferase ◽  
Time Courses ◽  
Apparently Healthy

Abstract We report the intermediate-term effects of three consecutive evenings of moderate ethanol ingestion (0.75 g/kg body weight each evening) on activity values for alkaline phosphatase, gamma-glutamyltransferase, creatine kinase, aspartate aminotransferase, alanine aminotransferase, and lactate dehydrogenase in sera of nine apparently healthy young adults. We define "intermediate-term" effects as those occurring between 10 h and 100 h after completion of the ethanol consumption schedule. The most pronounced changes in enzyme activity for the group of volunteers were: gamma-glutamyltransferase, +25% at 60 h after ethanol ingestion; alanine aminotransferase, +12% at 60 h after ethanol; and aspartate aminotransferase,--12% at 60 h after ethanol. All three enzymes exhibited similar time courses, i.e., mean peak activity changes were observed at 60 h, and all three mean enzyme activity values returned to near baseline by 100 h. The possible explanations for the observed changes and the clinical significance are discussed.

scholarly journals Overexpression of miRNA-21 Promotes the Proliferation and Invasion in Hepatocellular Carcinoma Cells via Suppressing SMAD7

2019 ◽  
Vol 18 ◽  
pp. 153303381987868 ◽  
Author(s):  
Yan Wang ◽  
Ping Zhang ◽  
Mei Yuan ◽  
Xiaojie Li
Keyword(s):  
Hepatocellular Carcinoma ◽  
Family Member ◽  
Reporter Gene ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Hepatoma Cell ◽  
Luciferase Reporter ◽  
Control Group ◽  
Luciferase Reporter Gene ◽  
Proliferation And Invasion

Purpose: This study aimed to explore the molecular mechanism of microRNA-21 and smad family member 7 in hepatocellular carcinoma. Method: A total of 57 participants were divided into control group (healthy participants, n = 10) and hepatocellular carcinoma group (hepatocellular carcinoma patients, n = 37). The expression of microRNA-21 levels were first detected in these two groups. Cell transfection was performed on hepatoma cell lines, followed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and Transwell assay to reveal proliferation and invasion ability. Furthermore, the relation between microRNA-21 and smad family member 7 was revealed by luciferase reporter gene and RNA immunoprecipitation assay. Finally, a transplantation tumor model of breast cancer in mice was constructed. Results: The serum indicators including α-alanine aminotransferase, aspartate aminotransferase, and albumin were differentially expressed between hepatocellular carcinoma group and control group. Compared to the control group, there was a high expression of microRNA-21 in hepatocellular carcinoma group. Low expression of microRNA-21 inhibited the proliferation and invasion of HepG2.2.15 and Huh7-1.3 cells. Luciferase reporter gene and RNA innumoprecipitation assay showed that smad family member 7 was the target gene of microRNA-21. Moreover, mice model analysis showed that microRNA-21 might regulate the growth of the transplanted tumors in mice by targeting smad family member 7. Conclusion: The upregulated microRNA-21 might participate in the proliferation and migration in cells of hepatocellular carcinoma via suppression of smad family member 7. Furthermore, serum indicators such as alanine aminotransferase, aspartate aminotransferase, and albumin might be used as serum diagnostic markers for hepatocellular carcinoma.

scholarly journals Influence of Eclipta alba on serum alanine aminotransferase and aspartate aminotransferase activity in rabbits

2018 ◽  
Vol 7 (7) ◽  
pp. 1243
Author(s):  
B. P. Kale ◽  
Mujawar Jahir Rauf
Keyword(s):  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Treated Group ◽  
Biologically Active ◽  
Control Group ◽  
Serum Alanine Aminotransferase ◽  
Eclipta Alba ◽  
Group A ◽  
High Doses ◽  
Group B

Background: Paracetamol is a recognized antipyretic, analgesic drug which produces hepatic necrosis in high doses. Eclipta alba elaborates a vast array of biologically active compounds that are chemically diverse and structurally complex.Methods: Randomized open controlled experimental study Estimated levels of Serum aspartate aminotransferase (AST), Serum alanine aminotransferase (ALT) and Hepatoprotective action of in High doses of Paracetamol on serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activity.Results: ALT in all the groups including Control group (A) was (51.8±4.56IU/L). Paracetamol treated group (B) the ALT level increased at 48 hours and continued to be high up to 60 days (136.4±20.73IU/L) then decreased to (113.7±11.35IU/L) at 90 days. AST in all the groups including Control group (A) was (22.5±1.23IU/L). Appropriate antioxidant in appropriate doses as a matter of routine whenever hepatotoxic or potentially hepatotoxic drugs are prescribed. In Paracetamol treated group (B) the AST level increased at 48 hours and continued to be high up to 60 days (99.4±9.73IU/L) then decreased to (85.4±7.39IU/L) at 90 days.Conclusions: Appropriate antioxidant in appropriate doses as a matter of routine whenever hepatotoxic or potentially hepatotoxic drugs are prescribed.

scholarly journals Protein-synthesizing function and functional state of the liver of dogs at experimental toxocariasis

2020 ◽  
Vol 22 (98) ◽  
pp. 132-137
Author(s):  
W. S. Said ◽  
V. V. Stybel ◽  
B. V. Gutyj ◽  
O. B. Pryima ◽  
I. Y. Mazur
Keyword(s):  
Body Weight ◽  
Functional State ◽  
Total Protein ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Experimental Studies ◽  
Control Group ◽  
Activity Of Enzymes ◽  
Experimental Group

The aim of the study was to investigate the effect of toxocariasis invasion on protein-synthesizing function and functional state of the liver of dogs. For experimental studies, 12 dogs aged from two to four months were used and two groups of six animals were formed in each: control and experimental. Puppies of the experimental group were experimentally infected with the pathogen toxocariasis at a dose of 5,000 invasive eggs of T. canis per kg of body weight. The control puppies were clinically healthy. Protein-synthesizing function of the liver of dogs in experimental toxocariasis was studied by the level of total protein and its fractions. It was found that on the 25th day of the experiment the level of total protein in the blood of the experimental group of dogs was 56.9 ± 1.47 g/l, while in the control group – 63.8 ± 2.92 g/l. The lowest level of the studied indicator was on the 30th day of the experiment in the blood of the experimental group of dogs, where it decreased by 9.6 %. The results of a study of protein fractions in the blood of dogs infested with toxocara showed that the percentage of albumin is likely to decrease. However, in the blood of infected dogs in this period of research there was a probable increase in globulin levels. The study of the effect of toxocariasis invasion on the activity of enzymes in blood serum is a test for the morphological and functional state of tissues and organs. Based on the studies, it was found that with the development of toxocariasis invasion in dogs there is an increase in alanine aminotransferase in their serum. On the 25th and 30th day of the experiment, the activity of the enzyme in the serum of infected dogs was the highest, where compared with the control group of dogs, the activity of ALT increased by 51.2 and 59.1 %, respectively. Similar changes in activity were observed in the determination of aspartate aminotransferase, where, respectively, on the 15th, 20th and 25th day of the experiment, the activity of AST in the serum of infected dogs increased by 19.3, 27.5 and 39.1 %.

scholarly journals Effect of Ivermectin on semen parameters and levels of Alanine aminotransferase (ALT) and Aspartate aminotransferase (AST) enzymes in seminal plasma of Iraqi Awassi rams

2014 ◽  
Vol 13 (1) ◽  
pp. 115
Author(s):  
Ali. J. Ali
Keyword(s):  
Seminal Plasma ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Semen Quality ◽  
Harmful Effect ◽  
Sperm Concentration ◽  
Semen Parameters ◽  
Control Group ◽  
Volume Percentage ◽  
Semen Collection

The objective of this study was to evaluate the effect of Ivermectin on semen quality and estimation of alanine aminotransferase (ALT), and aspartate aminotransferase (AST) in seminal plasma. Six mature Awassi rams aged 2-3 years and weighing 38-42 Kg were used in this study. Semen collection was done weekly for five weeks from the same ram. The first two collection considered as training for the animal and discarded, the second two collection were considers for the control group. After administration of the drug (Ivermectin), semen samples were collected from the Awassi ram 1, 7,14 days. The parameter studies were included semen volume, percentage motility of sperm, sperm concentration, live sperm, and morphology percentage. Samples of seminal plasma were analyzed for the estimation of alanine aminotransferase (ALT), aspartate aminotransferase (AST). Results of this study indicate that Ivermectin leads to significant (P<0.05) decreased in semen parameters after the first semen collection. Reaction time significantly higher (P<0.05) in the treated group after 1 day of injection. Alanine aminotransferase ALT, Aspartate aminotransferase AST show no significant differences in activities in seminal plasma during the study. In conclusion: it is preferable to use the animals for the purpose of reproduction only after at least 7 days after Ivermectin injection due to it harmful effect on semen quality.

scholarly journals Occupational effect of spray painting fumes on some biochemical parameters in automobile spray painters in Yenagoa Bayelsa State, Nigeria

2021 ◽  
Vol 9 (2) ◽  
pp. 038-044
Author(s):  
Emmanuel Tonbra Egoro ◽  
Emmanuel Sunday Oni ◽  
Jonathan Ebipade Lawrence
Keyword(s):  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Biochemical Parameters ◽  
Control Group ◽  
Chemical Toxicity ◽  
C Reactive Protein ◽  
Spray Painting ◽  
Plasma Urea ◽  
Reactive Protein ◽  
Experimental Group

Automobile spray painters are often prone to chemical toxicity due to their routine work. This study was aimed at the occupational effect of spray painting fumes on some biochemical parameters in automobile spray painters. Five milliliters of blood specimen were collected into lithium heparin anti-coagulated bottles from twenty five automobile spray painters with ≤ 10 years working experience (experimental group one), ≥ 11 years working experience (experimental group two) and non-automobile spray painters which were monitored as control group respectively. After this, plasma alanine aminotransferase, aspartate aminotransferase, C-reactive protein, urea and creatinine were measured quantitatively using a spectrophotometer. The mean values of all the measured biochemical parameters showed no statistically significant differences (p>0.05) in the automobile spray painters with ≤ 10 years working experience as compared with that of the control group with the exception of plasma C-reactive protein, while those with ≥ 11 years working experience showed statistically significant differences (p<0.05) in plasma alanine aminotransferase, aspartate aminotransferase and C-reactive protein however, the plasma urea and creatinine showed no statistically significant differences (p>0.05). In conclusion, the concentration of plasma alanine aminotransferase, aspartate aminotransferase and C-reactive protein may be altered in automobile spray painters with ≥ 11 years working experience, thus these biochemical parameters should be monitored regularly in spray painters within this category of working experience

scholarly journals The dynamics of the liver protein-producting function and cytolysis processes during the period of acute reaction to a combined cranio-skeletal trauma

2013 ◽  
Vol 17 (2 (66)) ◽  
pp. 29-33
Author(s):  
A. I. Gozhenko ◽  
R. M. Borys ◽  
A. A. Hudyma
Keyword(s):  
Liver Function ◽  
Characteristic Feature ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Control Group ◽  
Liver Protein ◽  
Skeletal Trauma ◽  
Acute Reaction ◽  
Post Traumatic ◽  
Experimental Group

Experimental cranioskeletal trauma stipulates an impairment of the protein-synthesizing liver function, which is especially noticeable in 12 hours of the post-traumatic period in animals with additional bleeding. The activity of the blood serum aspartate- and alaninaminotransferase essentially exceeds the control levels during all the terms of observation. A characteristic feature of aspartate aminotransferase dynamics in animals with a cranioskeletal trauma without bleeding is reaching by it a statistically significantly higher level pertaining to the control group already in 2 hours, which remains consistently high until the end of the experiment. It elevates in the group with bleeding in the dynamics of the posttraumatic period, and in 24 hours and it essentially exceeds the level present in the experimental group of animals without bleeding. This pattern is also observed in relation to the alanine aminotransferase value.

scholarly journals Assessment of selected biochemical parameters among food hawkers along the streets of Yenagoa Bayelsa State, Nigeria

2021 ◽  
Vol 16 (3) ◽  
pp. 103-108
Author(s):  
Emmanuel Tonbra Egoro ◽  
Emmanuel Sunday Oni ◽  
Otaraku Jonathan Oye ◽  
Annabel Awele Idama
Keyword(s):  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Biochemical Parameters ◽  
Control Group ◽  
C Reactive Protein ◽  
Mean Values ◽  
Reactive Protein ◽  
Significant Difference ◽  
The Mean ◽  
Experimental Group

This study was aimed on assessment of selected biochemical parameters among food hawkers along the streets of Yenagoa, Bayelsa State, Nigeria. Five milliliter of blood specimen was collected from fifteen food hawkers with ≤ 5 years working experience (experimental group one), ≥ 5 years working experience (experimental group two) and nonfood hawkers (control group) respectively into lithium heparin anti-coagulated bottles. Thereafter alanine aminotransferase, aspartate aminotransferase, urea, creatinine and C-reactive protein were measured quantitatively. The mean values of volunteers in experimental group one showed no statistically significant difference (p>0.05) in alanine aminotransferase (7.46 ± 1.02), aspartate aminotransferase (7.02 ± 0.86), urea (7.70 ± 1.29) and creatinine (70.44 ± 3.54) as compared with the control group (7.42 ± 0.98), (6.98 ± 0.82), (7.65 ± 1.28) and (70.40 ± 3.52) respectively, but there was a statistically significant difference (p<0.05) in C-reactive protein (17.40 ± 1.98) when compared with the control group (2.70 ± 0.70). However, the mean values of food hawkers in experimental group two showed statistically significant difference (p< 0.05) in all the measured biochemical parameters 27.70±2.98, 21.40±2.06, 15.50±2.02, 110.70±3.74, 29.42±3.20 as compared with that of the control group 7.42±0.98, 6.98±0.82, 7.65±1.28, 70.40±3.52, 2.74±0.70. The volunteers in experimental groups one and two showed 13% -27% and 53% -80% abnormal values of the measured biochemical parameters respectively as compared with that of the control group. In conclusion, alanine aminotransferase, aspartate aminotransferase, urea, creatinine and C-reactive protein may be altered in food hawkers with ≥ 5 years working experience. It is therefore recommended that food hawkers in this category should go for hepato-renal and inflammatory biochemical parameters checkup occasionally in any registered and licensed Medical Laboratory Facility.

scholarly journals Toxicological Effects of Aqueous Extract From African Walnut (Tetracarpidium conophorum) Leaves in Rats

2017 ◽  
Vol 22 (4) ◽  
pp. 919-925 ◽  
Author(s):  
Seun F. Akomolafe ◽  
Ganiyu Oboh ◽  
Sunday I. Oyeleye ◽  
Tosin A. Olasehinde
Keyword(s):  
Uric Acid ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Biochemical Parameters ◽  
Inflammatory Cell ◽  
Morphological Changes ◽  
Control Group ◽  
Toxicological Effects ◽  
Significant Difference ◽  
Liver And Kidney

Tetracarpidium conophorum leaves are used in traditional medicine for the treatment of male infertility, without considering its toxicity and side effects. In this study, we investigated the effects of T conophorum leaves on some biochemical parameters such as alanine aminotransferase, aspartate aminotransferase, bilirubin, albumin, creatinine, and uric acid. Histology of the liver and kidney were also assessed. The result revealed that the alanine aminotransferase and aspartate aminotransferase levels of the control group were not significantly different from the experimental groups. There was no significant difference in the albumin and bilirubin levels of the control and experimental groups. Similarly, the uric acid and creatinine levels of the experimental rats were not significantly different from the control. The examination of liver and kidney sections did not show any morphological changes and inflammatory cell infiltrations. These findings suggest that the leaves did not induce any pathological changes at the doses tested.

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