scholarly journals First Molecular Characterization of Colletotrichum sp. and Fusarium sp. Isolated from Mangrove in Mexico and the Antagonist Effect of Trichoderma harzianum as an Effective Biocontrol Agent

2021 ◽  
Vol 37 (5) ◽  
pp. 465-475
Author(s):  
Mayra I. Grano-Maldonado ◽  
Rosalio Ramos-Payan ◽  
Fernando Rivera-Chaparro ◽  
Maribel Aguilar-Medina ◽  
José Geovanni Romero-Quintana ◽  
...  
Keyword(s):  
Trichoderma Harzianum ◽  
Pathogenic Fungus ◽  
Mangifera Indica ◽  
Fungal Species ◽  
Avicennia Germinans ◽  
Molecular Characteristics ◽  
Mangrove Species ◽  
Southern Mexico ◽  
Red Mangrove ◽  
Fusarium Sp

The aim of this study was to characterize potential fungal species affecting mangrove species in Mexico. The phytopathogens were identified based on morphological and molecular characteristics using internal transcribed spacer (ITS1/ITS4) primers then sequenced and compared with the other related sequences in GenBank (NCBI). Three fungal species were identified as <i>Colletotrichum queenslandicum</i> (Weir and Johnst, 2012) from black mangrove (<i>Avicennia germinans</i>); <i>Colletotrichum ti</i> (Weir and Johnst, 2012) from white mangrove (<i>Laguncularia racemosa</i>) and buttonwood mangrove (<i>Conocarpus erectus</i>); <i>Fusarium equiseti</i> (Corda) from red mangrove (<i>Rhizophora mangle</i>). In addition, <i>C. ti</i> and <i>F. equiseti</i> were identified from mango <i>Mangifera indica</i> L. sampled close by the mangrove area. This study provides first evidence of anthracnose on four mangrove species caused by <i>Colletotrichum</i> and <i>Fusarium</i> species in the “Términos” coastal lagoon in Campeche State southern Mexico. This is the first time that <i>C. queenslandicum</i> and <i>C. ti</i> are reported in Mexico. <i>F. equiseti</i> has not been reported affecting <i>M. indica</i> and <i>R. mangle</i> until the present work. Little is known regarding fungal diseases affecting mangroves in Mexico. These ecosystems are protected by Mexican laws and may be threatened by these pathogenic fungus. This is the first report of the effect of <i>Trichoderma harzianum</i> TRICHO-SIN as an effective biological control against of <i>Colletotrichum</i> and <i>Fusarium</i> species.

scholarly journals A preliminary investigation of endophytic fungal diversity at Hope, East Coast Demerara, Guyana

2021 ◽  
Vol 17 (2) ◽  
pp. 044-048
Author(s):  
Kimberly Craig ◽  
Abdullah Ansari
Keyword(s):  
Fungal Diversity ◽  
East Coast ◽  
Preliminary Investigation ◽  
Fungal Endophytes ◽  
Avicennia Germinans ◽  
Laguncularia Racemosa ◽  
Mangrove Species ◽  
Red Mangrove ◽  
Fungal Isolates ◽  
White Mangrove

Foliar endophytic fungi spend a part of their life cycle on the leaves of plants. They may demonstrate no apparent symptoms but may also cause disease at a later time in the plant’s life. Studies investigating foliar fungal endophytes of mangroves are limited. Therefore, the purpose of this study was to investigate the foliar fungal endophytes present on the leaves of three mangrove species: namely Red mangrove (Rhizophora mangle), Black mangrove (Avicennia germinans) and White mangrove (Laguncularia racemosa). The study site was an area located at Hope, East Coast Demerara, and South America, Guyana. Out of sixty (60) leaf samples that were prepared, fourteen (14) fungal isolates were identified. Most of the fungi isolated in the study were found to be Hyphomycetes (Aspergillus, Fusarium, Penicillium, Alternaria, Cladosporium and Curvularia) while the others were Zygomorphic (Mucor and Rhizopus). The ANOVA calculations for the isolates from the three mangrove species were found to not be statistically significant. R mangle was the preferred host out of the three (3) species. The findings of this study confirm that mangroves have rich endophytic diversity and demonstrate rich research and biochemical potential.

scholarly journals IDENTIFIKASI PENYAKIT LAYU PADA BIBIT GMELINA (Gmelina arborea Roxb.) DI PERSEMAIAN DAN UJI ANTAGONISME Trichoderma sp. SECARA IN-VITRO

2018 ◽  
Vol 8 (2) ◽  
pp. 50
Author(s):  
Yeni Nuraeni
Keyword(s):  
Trichoderma Harzianum ◽  
Pathogenic Fungus ◽  
Wilt Disease ◽  
Gmelina Arborea ◽  
Trichoderma Sp ◽  
Fusarium Sp

          Gmelina seedlings which were 1.5 months age in the nursery Center for Reseacrh and Development Forests Bogor were attacked by wilt disease over 4.27%. The purpose of study were to determine the patoghens that cause the wilt disease in the nursery and to study the antagonist Trichoderma sp. against the pathogen causing wilt by in vitro as control efforts. The identification result of disease causing wilt on the gmelina seedlings was pathogenic fungus namely Fusariumi sp. The percentage of antagonist test on Trichoderma harzianum (THA) and Trichoderma Cipanas (TC) inhibiting against Fusarium sp. were 40.31% and 33.55%, respectively.Keywords: Antagonistic, gmelina seedlings, Fusarium sp., wilt diseaseABSTRAK          Bibit gmelina umur 1,5 bulan di persemaian Pusat Penelitian dan Pengembangan Hutan Bogor mengalami serangan penyakit layu dengan persentase serangan sebesar 4,27%. Penelitian dilakukan untuk mengetahui patogen yang menyebabkan penyakit layu tersebut di persemaian dan melakukan uji antagonis Trichoderma sp.dengan patogen penyebab layu secara in-vitro sebagai upaya pengendaliannya. Hasil identifikasi penyebab penyakit layu pada bibit gmelina adalah cendawan patogen yaitu Fusarium sp. Sedangkan hasil uji antagonisme Trichoderma sp. berturut-turut Trichoderma harzianum (THA) dan Trichoderma Cipanas (TC) memiliki daya hambat terhadap Fusarium sp. sebesar 40,31% dan 33,55%.Kata kunci: Antagonis, bibit gmelina,Fusarium sp., penyakit layu

Brazilian peppertree and mangrove species response to foliar-applied novel auxin-type herbicides

2020 ◽  
Vol 13 (2) ◽  
pp. 102-107
Author(s):  
Stephen F. Enloe ◽  
James K. Leary ◽  
Candice M. Prince ◽  
Benjamin P. Sperry ◽  
Dwight K. Lauer
Keyword(s):  
Complex Structure ◽  
Avicennia Germinans ◽  
Freshwater Wetlands ◽  
Laguncularia Racemosa ◽  
Mangrove Species ◽  
Invasive Shrub ◽  
Species Response ◽  
Red Mangrove ◽  
Highly Sensitive ◽  
Geographic Remoteness

AbstractBrazilian peppertree (Schinus terebinthifolia Raddi) is an invasive shrub that is problematic in both freshwater wetlands and brackish mangrove communities. The complex structure, geographic remoteness, and general herbicide sensitivity of mangrove systems have resulted in great technical challenges for managers attempting selective S. terebinthifolia control. Recent advances in auxin herbicide technologies warrant herbicide screening to address this growing problem. Therefore, greenhouse experiments were conducted in 2018 and 2019 to evaluate four non-target mangrove species and S. terebinthifolia response to the three herbicides: aminocyclopyrachlor, aminopyralid, and florpyrauxifen-benyzl. Aminocyclopyrachlor controlled S. terebinthifolia, but was highly injurious to black mangrove [Avicennia germinans (L.) L.], red mangrove (Rhizophora mangle L.), white mangrove [Laguncularia racemosa (L.) C.F. Gaertn.], and buttonwood mangrove (Conocarpus erectus L.). Aminopyralid also controlled S. terebinthifolia but its impact varied across mangrove species. Laguncularia racemosa and C. erectus were highly sensitive to aminopyralid, R. mangle exhibited dose-dependent tolerance, and A. germinans was highly tolerant. Florpyrauxifen-benzyl failed to control S. terebinthifolia and resulted in severe injury to all four mangrove species. These results indicate differential responses to newer auxins in both the target response and non-target plant community of interest. The efficacy of aminopyralid on S. terebinthifolia, coupled with its selectivity on A. germinans warrants further testing.

scholarly journals Forest Structure and Projections of Avicennia germinans (L.) L. at Three Levels of Perturbation in a Southwestern Gulf of Mexico Mangrove

Forests ◽  
2021 ◽  
Vol 12 (8) ◽  
pp. 989
Author(s):  
Basáñez-Muñoz Agustín de Jesús ◽  
Jordán-Garza Adán Guillermo ◽  
Serrano Arturo
Keyword(s):  
Size Structure ◽  
Transition Probabilities ◽  
High Sensitivity ◽  
Avicennia Germinans ◽  
Mangrove Forests ◽  
Mangrove Species ◽  
Adult Trees ◽  
Mangrove Dynamics ◽  
Projection Matrices ◽  
Partial Tree

Mangrove forests have declined worldwide and understanding the key drivers of regeneration at different perturbation levels can help manage and preserve these critical ecosystems. For example, the Ramsar site # 1602, located at the Tampamachoco lagoon, Veracruz, México, consists of a dense forest of medium-sized trees composed of three mangrove species. Due to several human activities, including the construction of a power plant around the 1990s, an area of approximately 2.3 km2 has suffered differential levels of perturbation: complete mortality, partial tree loss (divided into two sections: main and isolated patch), and apparently undisturbed sites. The number and size of trees, from seedlings to adults, were measured using transects and quadrats. With a matrix of the abundance of trees by size categories and species, an ordination (nMDS) showed three distinct groups corresponding to the degree of perturbation. Projection matrices based on the size structure of Avicennia germinans showed transition probabilities that varied according to perturbation levels. Lambda showed growing populations except on the zone that showed partial tree loss; a relatively high abundance of seedlings is not enough to ensure stable mangrove dynamics or start regeneration; and the survival of young trees and adult trees showed high sensitivity.

scholarly journals Morpho-molecular identification and first report of Fusarium equiseti in causing chilli wilt from Kashmir (Northern Himalayas)

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ammarah Hami ◽  
Rovidha S. Rasool ◽  
Nisar A. Khan ◽  
Sheikh Mansoor ◽  
Mudasir A. Mir ◽  
...  
Keyword(s):  
Dna Barcoding ◽  
Pcr Amplification ◽  
Its Region ◽  
Fungal Species ◽  
Pathogenicity Test ◽  
Kashmir Valley ◽  
Fusarium Equiseti ◽  
Infected Root ◽  
Fusarium Sp ◽  
High Degree

AbstractChilli (Capsicum annuum L.) is one of the most significant vegetable and spice crop. Wilt caused by Fusarium Sp. has emerged as a serious problem in chilli production. Internal transcribed spacer (ITS) region is widely used as a DNA barcoding marker to characterize the diversity and composition of Fusarium communities. ITS regions are heavily used in both molecular methods and ecological studies of fungi, because of its high degree of interspecific variability, conserved primer sites and multiple copy nature in the genome. In the present study we focused on morphological and molecular characterization of pathogen causing chilli wilt. Chilli plants were collected from four districts of Kashmir valley of Himalayan region. Pathogens were isolated from infected root and stem of the plants. Isolated pathogens were subjected to DNA extraction and PCR amplification. The amplified product was sequenced and three different wilt causing fungal isolates were obtained which are reported in the current investigation. In addition to Fusarium oxysporum and Fusarium solani, a new fungal species was found in association with the chilli wilt in Kashmir valley viz., Fusarium equiseti that has never been reported before from this region. The studies were confirmed by pathogenicity test and re-confirmation by DNA barcoding.

Biocontrol of seed-borne Alternaria raphani and A. brassicicola

1987 ◽  
Vol 33 (10) ◽  
pp. 850-856 ◽  
Author(s):  
G. Vannacci ◽  
G. E. Harman
Keyword(s):  
Biological Control ◽  
Host Range ◽  
Trichoderma Harzianum ◽  
Pythium Species ◽  
Biological Control Agents ◽  
Fusarium Sp ◽  
Chamber Studies ◽  
Growth Chamber Studies ◽  
Radish Seedlings

Forty-two microorganisms were tested as biological control agents against Alternaria raphani and A. brassicicola. Tests were conducted for in vitro antagonistic ability, for ability to control the pathogens on naturally infected seeds germinated on moistened blotters, and in planting mix in growth chamber studies, and for their ability to reduce pod infection. The organisms tested were obtained from cruciferous seeds or were strains already identified as being effective against soil-borne Pythium species. The blotter test indicated that six organisms increased both the number of healthy seedlings and the number of seedlings produced from A. raphani infected radish seeds. An additional seven strains improved either germination or increased the number of healthy seedlings. Twenty-nine organisms increased the number of healthy cabbage seedlings from A. brassicicola infected seeds, but total germination was not modified by any treatment. Experiments in planting mix showed that five antagonists (Chaetomium globosum, two strains of Trichoderma harzianum, T. koningii, and Fusarium sp.) increased the number of healthy plants in both radish samples tested, while four additional antagonists provided a significant increase in only one of the samples tested. The five antagonists that consistently increased numbers of healthy radish seedlings also decreased pod infection by A. raphani. None were as effective as iprodrone, however. Several effective antagonists were found to be mycoparasitic against Alternaria spp. Some strains of Trichoderma previously found to be effective against Pythium spp. were also effective against Alternaria spp., indicating that these strains have a wide host range.

scholarly journals Epidemiology of Mango Malformation in Guerrero, México, with Traditional and Integrated Management

Plant Disease ◽  
1999 ◽  
Vol 83 (3) ◽  
pp. 223-228 ◽  
Author(s):  
D. H. Noriega-Cantú ◽  
D. Téliz ◽  
G. Mora-Aguilera ◽  
J. Rodríguez-Alcazar ◽  
E. Zavaleta-Mejía ◽  
...  
Keyword(s):  
Wind Speed ◽  
Integrated Management ◽  
Disease Incidence ◽  
Mangifera Indica ◽  
Spore Density ◽  
Cost Rate ◽  
Average Maximum ◽  
Fusarium Sp ◽  
Epidemic Development ◽  
Benefit Cost

The temporal progress of malformation (MM) of mango (Mangifera indica) was studied from 1993 to 1995 with three management technologies applied to commercial plantations in North Guerrero, Mexico. Management influenced shoot production and thus determined the dynamics of epidemics. Environmental factors also affected disease incidence, particularly through an apparent effect on inoculum dispersal. In general, integrated management (IM), consisting of pruning, acaricide, and fungicide sprays, resulted in slower rates of epidemic development, lower levels of initial and final disease, and lesser areas under the disease progress curves. In the first cycle, IM increased yield per tree by 51% in relation to high technology (HT) and 74% in relation to lower traditional technology (LT), representing a benefit-cost rate of 2.8 and 3.3, respectively. Change of malformation incidence was correlated positively with the number of macroconidia of Fusarium sp. trapped in the canopy (r = 0.90, P = 0.0001) and wind speed (r = 0.83, P = 0.0001); both variables lagged over a 4-month period. The greatest change in malformation occurred during the main vegetative flush, which occurred 3 to 6 months after picking the fruit (May). The accumulated proportion of diseased shoots was correlated with the following variables measured over a 1-week period: average maximum daily temperature (r = -0.68, P = 0. 01), average temperature per hour (r = -0.59, P = 0.04), average number of hours with relative humidity ≥60% (r = -0.82, P = 0.001), and wind speed (r = 0.94, P = 0.0001). In general, the greatest spore density was found during the rainy season, with a morning periodicity showing the highest correlation with wind speed (r = 0.812, P = 0.0001). F. subglutinans was isolated consistently from diseased (86%) and asymptomatic (5%) vegetative and flowering shoots.

scholarly journals Isolation and identification of phylloplane and endophytic fungi from Sapindus mukorossi

2020 ◽  
Vol 26 (2) ◽  
Author(s):  
B.D. Varpe
Keyword(s):  
Endophytic Fungi ◽  
Terrestrial Ecosystem ◽  
Fungal Species ◽  
Penicillium Expansum ◽  
Isolation And Identification ◽  
Epicoccum Nigrum ◽  
Candida Sp ◽  
Fusarium Sp ◽  
The Rich ◽  
High Level

Phylloplane biodiversity and endophytic fungi is considered one of the rich origins of novel biological activity compounds and high-level structural variation on the leaf surface. Plant leaves surface is a diverse terrestrial ecosystem, including filamentous fungi. This study aims to study the isolation and enumeration of Sapindus mukorossi phylloplane and endophytic fungal diversity. The Sapindus mukorossi isolated 14 fungal species from 9 genera of phylloplane and endophytic fungi. Cladosporium herbarum, Penicillium expansum, Fusarium oxysporum, Fusarium sp., Alternaria alternate, Collectotrichum orbiculare, Torulla herbarium, Epicoccum nigrum and Candida sp. as a phylloplane fungi. Aspergillus niger, A. flavus, Epicoccum nigrum. Penicillum digitatum, Penicillum sp. were identified as endophytic fungi.

scholarly journals Air Quality Assessment of Port Harcourt Urban Slums and Health Implications

2021 ◽  
pp. 50-57
Author(s):  
H. O. Stanley ◽  
M. E. Amesi
Keyword(s):  
Air Quality ◽  
Fungal Species ◽  
Urban Slums ◽  
Candida Sp ◽  
Suspended Particulate ◽  
Port Harcourt ◽  
Microbiological Parameters ◽  
Fusarium Sp ◽  
Aspergillus Sp ◽  
Rhizopus Sp

This study was conducted to assess the outdoor air quality of some urban slums in Port Harcourt. Six sampling sites were selected, from the Port Harcourt urban slums; two sites from each slum represented with a suffix 1 or 2.  The slums are designated Marine base (#1 and #2), RSU BG, Obudu 2, Bundu (#1 and #2). The air quality was analyzed using portable handheld air quality analyzer and the microbiological parameters were determined by standard cultural method. The study revealed that the sampled sites were laden with bacterial and fungal species. namely; Klebsiella sp., Micrococcus sp., Escherichia sp., Pseudomonas sp., Baccilus sp., Aeromonas sp., Streptococus sp., Serratia sp., Aerococcus sp., Proteus sp. Penicillium sp., Fusarium sp., Candida sp., Aspergillus sp., Mucor sp., Rhizopus sp. and Tricorderma sp. Highest obtained noise level was at Marine base 1 which was  66 db, highest relative humidity of 54.8% at RSU BG, CO2  (ppm) values of 4.8, 80, 796, 850, 638, 698 for Marine base 2, Marine base 1, Obudu 2, RSU BG, Bundu 1 and Bundu 2 respectively. The values for NO2 (ppm) was (0.05, 0.053, 0.071, 0.022, 0.035, 0.023), suspended particulate matter (ppm) was (7.1, 8.7, 9.5, 9.5, 6.2, 6.2), SO2 (ppm) was (0.42, 0.15, 0.50, 0.34, 1.26, 0.41) CO (ppm) was (4.8, 1.7, 2.2, 3.0, 3.9, 3.6) and volatile organic compound (ppm) was (1.0, 1.1, 0.9, 75 and 1.2). This study has shown that Port Harcourt urban slums are experiencing some degree of contamination not acceptable for healthy living that requires attention to curb. These areas require all-round improvement in sanitation.   M Give one sentence on methodology.

scholarly journals IDENTIFIKASI DAN UJI PATOGENISITAS CENDAWAN ENTOMOPATOGEN LOKAL TERHADAP Leptocorisa oratorius

EUGENIA ◽  
2011 ◽  
Vol 17 (3) ◽  
Author(s):  
Emmy Senewe ◽  
Guntur Manengkey
Keyword(s):  
Fungal Pathogen ◽  
Fungal Pathogens ◽  
Pathogenic Fungus ◽  
Pathogenic Fungi ◽  
North Sulawesi ◽  
Pathogenicity Tests ◽  
Major Pest ◽  
White Mycelium ◽  
Fusarium Sp ◽  
Leptocorisa Oratorius

ABSTRACT Leptocorisa oratorius is one major pest of rice in North Sulawesi. Hence, it is necessary to control the pest. The research objective was to identify and to test pathogenicity of local  entomopathogen fungi which infected  Leptocorisa oratorius. The pathogens were collected through sampling of L. oratorius which had been infected by the fungi in the field. The pathogenic fungi was isolated using PDA medium, identified followed by inoculation for pathogenecity test.  During several sampling pest, it was found that  L. oratorius was attacked by fungal pathogens in the field. The identification revelead that the fungal pathogens were Beauveria sp and Fusarium sp. Both the fungal pathogen produced white mycelium and could only be distinguished using microscope in the laboratory. Result of pathogenicity tests showed that the two fungal pathogens caused different mortality of the L. oratorius. Mortality of  L. oratorius caused by pathogenic fungus Beauveria sp was  30.3% . Whereas, mortality of  L. oratorius caused by Fusarium sp was only 3.33%. Keywords : pathogenic fungi, entomopathogen, pathogenicity tests, L. oratorius

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