scholarly journals Protective Effect of Dealcoholized Persimmonwine on H2O2 - Induced Oxidative Injury in H9c2 Cardiomyocytes

2013 ◽  
Vol 2 (4) ◽  
pp. 61
Author(s):  
Jin Taek Hwang ◽  
Chan Kyu Han ◽  
Sang Yoon Choi ◽  
Sung Soo Kim
Keyword(s):  
Oxidative Stress ◽  
Gallic Acid ◽  
High Performance ◽  
Cell Injury ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Dependent Manner ◽  
Protective Effects ◽  
H9c2 Cardiomyocytes ◽  
Dose Dependent

<p>In this study, we investigated the antioxidant capacity of persimmon wine (PW) and dealcoholized persimmon wine (DPW). Both PW and DPW showed radical scavenging activity in the DPPH (1-diphenyl-2-picrylhydrazyl) assay. We next analyzed the phenolic content and major compounds present in PW using high-performance liquid chromatography (HPLC). Phenolic compounds, including gallic acid, catechin, and epicatechin, were found in PW. Gallic acid was the most abundant phenolic compound (157.5 µg/ml) in PW. In addition, the protective effects of DPW and gallic acid against H<sub>2</sub>O<sub>2</sub>-induced cell injury in H9c2 cardiomyocytes were investigated. Pretreatment with DPW or gallic acid strongly inhibited H<sub>2</sub>O<sub>2</sub>-induced cell death in a dose-dependent manner. These results suggested that PW and its major phenolic component, gallic acid, were effective inhibitors of oxidative stress and oxidative stress-induced cardiomyocyte injury.</p>

scholarly journals A Comparative Study of the Antioxidative Effects of Helichrysum italicum and Helichrysum arenarium Infusions

Antioxidants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 380
Author(s):  
Katja Kramberger ◽  
Zala Jenko Pražnikar ◽  
Alenka Baruca Arbeiter ◽  
Ana Petelin ◽  
Dunja Bandelj ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
High Performance ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Morphological Type ◽  
Mass Spectrometry Analysis ◽  
In Vitro Assays ◽  
Spectrometry Analysis ◽  
Stress Related Genes

Helichrysum arenarium (L.) Moench (abbrev. as HA) has a long tradition in European ethnomedicine and its inflorescences are approved as a herbal medicinal product. In the Mediterranean part of Europe, Helichrysum italicum (Roth) G. Don (abbrev. as HI) is more common. Since infusions from both plants are traditionally used, we aimed to compare their antioxidative potential using in vitro assays. Two morphologically distinct HI plants, HIa and HIb, were compared to a commercially available HA product. Genetic analysis using microsatellites confirmed a clear differentiation between HI and HA and suggested that HIb was a hybrid resulting from spontaneous hybridization from unknown HI subspecies. High-performance liquid chromatography–mass spectrometry analysis showed the highest amounts of hydroxycinnamic acids and total arzanol derivatives in HIa, whereas HIb was richest in monohydroxybenzoic acids, caffeic acids, and coumarins, and HA contained the highest amounts of flavonoids, especially flavanones. HIa exhibited the highest radical scavenging activity; it was more efficient in protecting different cell lines from induced oxidative stress and in inducing oxidative stress-related genes superoxide dismutase 1, catalase, and glutathione reductase 1. The antioxidative potential of HI was not only dependent on the morphological type of the plant but also on the harvest date, revealing important information for obtaining the best possible product. Considering the superior properties of HI compared to HA, the evaluation of HI as a medicinal plant could be recommended.

scholarly journals Hydroxyl-radical scavenging activity of hydrogen does not significantly contribute to its biological function

2021 ◽  
Author(s):  
Qinjian Li ◽  
Fei Xie ◽  
Yang Yi ◽  
Pengxiang Zhao ◽  
Xin Zhang ◽  
...  
Keyword(s):  
Hydroxyl Radicals ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Published Data ◽  
Oh Radicals ◽  
Dependent Manner ◽  
Protective Effects ◽  
Beneficial Effects ◽  
Wide Range ◽  
Depth Study

AbstractSince Ohsawa et al. reported a biological antioxidant function of hydrogen in 2007, researchers have now shown it to exert protective effects in a wide range of human and animal disease models. Clinical observations and scientific arguments suggest that a selective scavenging property of H2 cannot adequately explain the beneficial effects of hydrogen. However, there is no experiment challenging the original published data, which suggested that molecular hydrogen dissolved in solution reacts with hydroxyl radicals in cell-free systems. Here we report that a hydrogen-saturated solution (0.6 mM) did not significantly reduce hydroxyl radicals in the Fenton system using 1 mM H2O2. We replicated the same condition as Ohsawa’s study (i.e. 5 μM H2O2), and observed a decrease in •OH radicals in both the H2-rich and N2-rich solutions, which may be caused by a decreased dissolved oxygen concentration. Finally, we determined the effect of hydrogen on a high-valence iron enzyme, horseradish peroxidase (HRP), and found that hydrogen could directly increase HRP activity in a dose-dependent manner. Overall, these results indicate that although H2 and •OH can react, the reaction rate is too low to have physiological function. The target of hydrogen is more complex, and its interaction with enzymes or other macro-molecules deserve more attention and in-depth study.

scholarly journals The Metabolite Urolithin-A Ameliorates Oxidative Stress in Neuro-2a Cells, Becoming a Potential Neuroprotective Agent

Antioxidants ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 177 ◽  
Author(s):  
Guillermo Cásedas ◽  
Francisco Les ◽  
Carmen Choya-Foces ◽  
Martín Hugo ◽  
Víctor López
Keyword(s):  
Oxidative Stress ◽  
Enzyme Inhibitor ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Monoamine Oxidase A ◽  
Mitochondrial Activity ◽  
Radical Scavenger ◽  
Dependent Manner ◽  
Urolithin A ◽  
Ic50 Values

Urolithin A is a metabolite generated from ellagic acid and ellagitannins by the intestinal microbiota after consumption of fruits such as pomegranates or strawberries. The objective of this study was to determine the cytoprotective capacity of this polyphenol in Neuro-2a cells subjected to oxidative stress, as well as its direct radical scavenging activity and properties as an inhibitor of oxidases. Cells treated with this compound and H2O2 showed a greater response to oxidative stress than cells only treated with H2O2, as mitochondrial activity (MTT assay), redox state (ROS formation, lipid peroxidation), and the activity of antioxidant enzymes (CAT: catalase, SOD: superoxide dismutase, GR: glutathione reductase, GPx: glutathione peroxidase) were significantly ameliorated; additionally, urolithin A enhanced the expression of cytoprotective peroxiredoxins 1 and 3. Urolithin A also acted as a direct radical scavenger, showing values of 13.2 μM Trolox Equivalents for Oxygen Radical Absorbance Capacity (ORAC) and 5.01 µM and 152.66 µM IC50 values for superoxide and 2,2-diphenyss1-picrylhydrazyl (DPPH) radicals, respectively. Finally, inhibition of oxidizing enzymes, such as monoamine oxidase A and tyrosinase, was also detected in a dose-dependent manner. The cytoprotective effects of urolithin A could be attributed to the improvement of the cellular antioxidant battery, but also to its role as a direct radical scavenger and enzyme inhibitor of oxidases.

scholarly journals Antioxidant And Antibacterial Activity Of Methanolic Extract Of Machilus Odoratissima

1970 ◽  
Vol 8 (1) ◽  
pp. 73-80 ◽  
Author(s):  
Amit Subedi ◽  
Mohan Prasad Amatya ◽  
Tirtha Maiya Shrestha ◽  
Shyam Kumar Mishra ◽  
Bharat Mani Pokhrel
Keyword(s):  
Antibacterial Activity ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antibacterial Properties ◽  
Dependent Manner ◽  
Scavenging Activity ◽  
Deoxy Sugar ◽  
Radical Method ◽  
Dose Dependent

The methanol extract of the bark of Machilus odoratissima was subjected to investigate its antioxidant and antibacterial properties. The phytochemical screening demonstrated the presence of different types of compound like terpenoids, tannins, deoxy sugar, saponins and phenolic compounds. The methanol extract of the plant was tested for antioxidant activity using scavenging activity of DPPH(1,1-diphenyl-2-picrylhydrazil) radical method and antibacterial activity against Staphylococcus aureus, Enterococcus faecalis, Escherichia coli & Pseudomonas aeruginosa bacteria using cup plate method. The extract exhibited high free radical scavenging activity. IC50 was found to be 3.37μg/ml. Antibacterial activity was observed against S. aureus in dose dependent manner DOI: http://dx.doi.org/10.3126/kuset.v8i1.6045 KUSET 2012; 8(1): 73-80

scholarly journals Assessment of Morus alba (mulberry) leaves extract for anti-psychotic effect in rats

2019 ◽  
Vol 8 (9) ◽  
pp. 2130
Author(s):  
Sriharsha Rayam ◽  
Babasaheb Laxmansing Kudagi ◽  
Madhavulu Buchineni ◽  
Rama Mohan Pathapati ◽  
Mallikarjuna Rao Immidisetty
Keyword(s):  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Morus Alba ◽  
Time Interval ◽  
Protective Agent ◽  
Dependent Manner ◽  
Anti Psychotic ◽  
Eye Infections ◽  
White Mulberry ◽  
Dose Dependent

Background: Morus alba commonly known as white mulberry has been widely cultivated to feed silkworms. This widely grown plant has been in use by tribals of this country for ailments such as asthma, cough, bronchitis, edema, insomnia, wound healing, diabetes, influenza, eye infections and nose bleeds. Various parts of morus alba linn are used as an cardioprotective, hepatoprotective anti-inflammatory, hypoglycemic, free radical scavenging activity and neuro-protective agent. In this study, anti-psychotic property of M. alba leaves extract (MAE) was evaluated by Haloperidol induced catalepsy model in rats.Methods: In this study Haloperidol induced catalepsy model was used to evaluate antipsychotic effects in rats. Haloperidol (1 mg/kg) was injected intraperitoneally to rats (n=6) pretreated with vehicle (0.5 mg/kg, i.p.) or MAE (100, 200 and 400 mg/kg, i.p).Results: In control treated animals, haloperidol produced the maximum catalepsy at 90 min 212.66 ±10.23. In animals treated with MAE at dose of 100 mg/kg, 200 mg/kg and 400 mg/kg significantly potentiated haloperidol induced catalepsy at each time interval, in a dose dependent manner. At dose 100, 200 and 400 mg/kg, animals treated with MAE showed maximum cataleptic score of 228.33±12.29, 265.66±7.33 and 274.16±8.86 respectively at 120 min (p<0.001).Conclusions: Results indicate that the MAE have anti-psychotic effects in haloperidol induced catalepsy model in rats.

Cyperus Iria Aqueous-Ethanol Extract Ameliorated Hyperglycemia, Oxidative Stress and Regulated Inflammatory Cytokines in Streptozotocin Induced Diabetic Rats

2021 ◽  
Author(s):  
Myeda Saeed ◽  
Ali Sharif ◽  
Saeed UlHassan ◽  
Bushra Akhtar ◽  
Faqir Muhammad ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
High Performance ◽  
Aqueous Ethanol ◽  
Ethanol Extract ◽  
Diabetic Rats ◽  
Dependent Manner ◽  
Good Tolerance ◽  
Stress Markers ◽  
Tnf Α ◽  
Dose Dependent

Abstract Present study is involved in identification of biophenolic and flavonoids from the aqueous-ethanol extract of Cyperus iria and appraisal of inflammatory and stress markers involved in endocrine dysfunction based upon its folktale use. Significantly higher quantities of phenolic (82.79 ± 0.003 mg/g GAE) and flavonoid (13.61 ± 0.002 mg/g QE) contents were present. Inhibitory concentration (IC50) exhibited an excellent potential for both antioxidant (IC50 = 3.22 µg/mL) and alpha amylase (IC50 = 36.29 µg/mL) inhibitory assays. High performance liquid chromatography (HPLC), confirmed the existence of myercetin, quercetin, kaempferol and ferullic acid. Cyperus iria aqueous-ethanol extract exhibits good tolerance against glucose. Streptozotocin induced hyperglycemia declined along with improvement in inflammatory (TNF-α = 15.6 ± 0.2 g/l, COX-2 = 357 ± 0.396 U/l, IL-6 = 572 ± 0.99 pg/l) and oxidative stress markers (SOD = 163 ± 0.616 and GSH-ST = 95.8 ± 0.44 U/mL) along with biochemical parameters in a dose-dependent manner. Present study suggests that Cyperus iria aqueous-ethanol extract possess hypoglycemic potential which might be attributed to the presence of phenolics and flavonoids.

Protective effect of chloroform extract of Stereospermum chelonoides bark against amyloid beta42 induced cell death in SH-SY5Y cells and against inflammation in Swiss albino mice

2018 ◽  
Vol 29 (6) ◽  
pp. 621-630
Author(s):  
Md. Imamul Islam ◽  
Meena Afroze Shanta ◽  
Milon Mondal ◽  
Nazia Hoque ◽  
Senjuti Majumder ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cell Death ◽  
Protective Effect ◽  
Antioxidant Capacity ◽  
Caspase 3 ◽  
Radical Scavenging ◽  
Chloroform Extract ◽  
Dependent Manner ◽  
Dose Dependent

Abstract Background This study was designed to evaluate the free radical scavenging property of chloroform extract of the bark of Stereospermum chelonoides (SCBC) and to investigate its potential in Alzheimer’s disease and inflammation, two oxidative stress related disorders. Methods Preliminary phytochemical analysis and in vitro antioxidant potential of SCBC were evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay, ferric reducing antioxidant power (FRAP) assay, cupric reducing antioxidant capacity (CUPRAC) and total antioxidant capacity determination assay. Total phenol and total flavonoid contents were also determined. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) based cytotoxicity and cyto-protective assays were performed on human neuroblastoma SH-SY5Y cells. Thioflavin-T assay and caspase activation measurement assay were carried out to elucidate the mechanism of cytoprotection of SCBC observed here. In vivo anti-inflammatory potential was measured using croton oil and xylene induced ear edema tests. Results Phytochemical screening of SCBC revealed the presence of various phytoconstituents. Dose-dependent in vitro antioxidant activity was observed. The extract was enriched in flavonoids and polyphenolic compounds too. SCBC was found to inhibit amyloid-β peptide 1-42 (Aβ42) induced cell death in a dose-dependent manner. Encouraged by the cyto-protective effect, its effects on Aβ42 fibrillogenesis and caspase-3 activated apoptosis were observed. SCBC significantly slowed down the Aβ42 fibrillogenesis and caspase-3 activation in a concentration-dependent manner indicating its probable mechanism of rendering cyto-protection. SCBC has been able to reduce inflammation significantly in croton oil induced ear edema in both doses. Conclusions Thus, this study could form the basis for further study for the potential use of SCBC in oxidative stress associated cell death and inflammation.

scholarly journals Canthium parviflorum Leaves: Antioxidant Activity in Food and Biological Systems, pH, and Temperature Stability

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Vanitha Reddy Palvai ◽  
Sowmya Mahalingu ◽  
Asna Urooj
Keyword(s):  
Antioxidant Activity ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Temperature Stability ◽  
Dependent Manner ◽  
Oil Emulsion ◽  
Dose Dependent

Canthium parviflorum leaves were analyzed for their proximate and phytochemical composition. The leaves were extracted with methanol (ME) and analyzed for antioxidant activity by radical scavenging method, reducing power, ferric reducing capacity, and in vitro inhibition of Fenton’s reagent induced oxidation in oil emulsion and microsomes. In addition, the effect of high temperature (100°C, 15 and 30 min) and pH (4.5, 7, and 9) on the antioxidant activity of ME was investigated. The leaves were rich in polyphenols, flavonoids β-carotene, glutathione, α-tocopherol, and ascorbic acid. The ME exhibited varying degree of antioxidant activity in a dose dependent manner. The RSA was 68%–500 μg. Reducing potency was 0.34 and FRAP was 1.377. Canthium exhibited greater inhibition of oxidation in microsomes (73%) than in the oil emulsion (21%). Heat treatment resulted in reduction of radical scavenging activity of extract from 68% to 40%. At pH 4.5 and 7 methanol extract exhibited some percent of antioxidant activity which ranged between 18 and 32%. Data indicates Canthium as a good source of antioxidants and methanol extract exhibited good antioxidant activity.

scholarly journals Cobalt Chloride Alters Mitochondrial Function in a Dose Dependent Manner

2021 ◽  
Vol 37 (3) ◽  
Author(s):  
Pham Thi Bich ◽  
Vu Thi Thu
Keyword(s):  
Oxidative Stress ◽  
Mitochondrial Function ◽  
Mitochondrial Membrane ◽  
Cobalt Chloride ◽  
Dose Dependence ◽  
Cell Counting ◽  
Dependent Manner ◽  
Mitochondrial Oxidative Stress ◽  
H9c2 Cardiomyocytes ◽  
Dose Dependent

Aim: This study was carried to evaluates of Cobalt chloride (CoCl2) on cardiac mitochondrial function. Methods: H9C2 cardiomyocytes were cultured in medium containing different concentrations of CoCl2. Cell viability, cardiolipin content, mitochondrial function, and mitochondrial oxidative stress were assessed by using Cell Counting Kit-8 and suitable fluorescence kits. Results: The obtained data showed that CoCl2 (200÷400 µM) induced cell death and decreased mitochondrial function of H9C2 cardiomyocytes in dose dependence. Especially, CoCl2 at dose of 300 µM significantly altered values of mitochondrial membrane potential, H2O2 and O2- to 63.79±2.15%, 145.81±5.83% and 143.10±3.07% (of 100% control), respectively. Conclusion: CoCl2 strongly induced cardiomyocyte death via altering mitochondrial function in a dose-dependent manner.

Anti-Oxiflammatory Profile of Date Extracts (Phoenix sylvestris)

2015 ◽  
Vol 2 (06) ◽  
pp. 297 ◽  
Author(s):  
Rintu Das ◽  
Pramathadhip Paul ◽  
Kaustab Mukherjee ◽  
Shinjini Mitra ◽  
Umesh Prasad Singh ◽  
...  
Keyword(s):  
Date Palm ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Dependent Manner ◽  
Human Embryonic Kidney Cell ◽  
Phenolic Contents ◽  
Different Types ◽  
Anti Oxidant ◽  
Dose Dependent

Fruit of date palm (Phoenix sylvestrisL.) is edible and used as an anti-geriatric, anti-oxidant ethnomedicine. In this study, three different types ofdateextracts, methanolic, acidic ethanolic and basic ethanolicwere evaluated for their putative in vitro scavenging effects on reactive oxygen species (ROS), where scavenging of hydroxyl radicals (basic ethanolic>acidic ethanolic>methanolic), superoxide radicals (acidic ethanolic>basic ethanolic>methanolic), DPPH radicals (acidic ethanolic>methanolic>basic ethanolic), nitric oxide (NO) (methanolic>acidic ethanolic>basic ethanolic) and inhibition of lipid peroxidation (basic ethanolic>acidic ethanolic> methanolic) were found to occur in a dose dependent manner. Their flavonoid and phenolic contents proved to be the source of this potent free radical scavenging activity and indicated a direct correlation with their total anti-oxidant capacity. On human embryonic kidney cell line (HEK) and murine RAW macrophages, bacterial lipopolysaccharide (LPS) induced inflammation, the date extracts applied therapeutically, inhibit intracellular oxidative stress significantly. This reinstatement of cellular homeostasis presumably occurs via mitochondrial pathways.

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